Method for preparing enzyme and bacterium composite agent for treating sewage and sludge

A technology of sewage sludge and compounding agent, which is applied in the field of preparation of enzyme-bacteria compounding agent, can solve the problems of treatment failure, ineffective effect, poor adaptability and synergistic effect of ordinary probiotics, etc.

Active Publication Date: 2011-11-23
康源绿洲微生物技术(北京)有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods generally have harsh conditions for microbial domestication and cultivation, the effect is not obvious when the sewage load is too large in the rainy season, and the application is not wide enough, as well as the shortcomings of large floor area and high equipment cost, so they cannot be widely used in the treatment of large areas of sewage sludge. project
[0011] The study believes that the main reason for the poor effect is that after the probiotics with decomposing ability are added to the sewage sludge to be treated, the high concentration of nitrogen, p

Method used

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  • Method for preparing enzyme and bacterium composite agent for treating sewage and sludge
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  • Method for preparing enzyme and bacterium composite agent for treating sewage and sludge

Examples

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preparation example Construction

[0053] The preparation method of the enzyme-bacteria composite agent for treating sewage sludge of the present invention can be divided into the following steps:

[0054] (1) Bacillus subtilis

[0055] 1. Seed culture: 6L of fermentation medium in 10L automatic seed fermentation tank, take the Bacillus subtilis cell suspension (about 10 9 cfu / ml) was inserted into the seed tank at 1% inoculum size, cultivated for 8 hours at 30° C., ventilation rate 1:1.2 volume ratio, 350 rpm.

[0056] Seed Medium: Nutrient Broth Agar Medium

[0057] Peptone 10.0g Beef Extract 3.0g NaCl 5.0g Agar 15.0g

[0058] Distilled water 1.0L pH 7.0

[0059] Natural pH value, sterilized at 121°C for 20 minutes.

[0060] 2. Fermentation culture: 60L of fermentation medium in 100L fully automatic stirring fermentation tank, inoculated by feed feeding from seed tank, continuous feeding for 5min, inoculum size 10%, 30°C, 300rpm, ventilation rate 1:0.4 volume ratio, culture for 8 hours Increase the venti...

experiment example 1

[0096] Experimental example 1. The water body of Xinzhou River in Shenzhen City is black and smelly, and the compound preparation of enzyme and bacteria is used to directly purify the emergency measures. The concentration of river water pollutants at the sampling point in front of Wuzhou Hotel was significantly reduced. Compared with the control, the average removal rate of COD was 59.3%, BOD5 was 51.1%, SS was 72.9%, TP was 52.3%, and NH3-N was 47.8%.

[0097] Experimental example 2. The black and odorous sewage in Gankeng and Shiyan areas of Shenzhen was treated, which eliminated the odor of the entire wetland system. The effluent water quality of the wetlands reached Class II water quality standards. It also improved the growth of crops on the constructed wetlands and extended the artificial The operating cycle of wetlands.

[0098] Analysis table of pretreatment results of digestion and acidification tank (unit: mg / L)

[0099]

experiment example 3

[0100] Experimental Example 3: The water out of the septic tank of Shenzhen Binhe Middle School is neither black nor smelly, the amount of sludge is reduced by 68.8%, COD is reduced by more than 75% on average, and the water quality is clear and transparent.

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Abstract

The invention relates to a method for preparing an enzyme and bacterium composite agent for treating sewage and sludge, which comprises the following steps: (1) preparing bacillus subtilis powder; (2) preparing bacterial solution of photosynthetic bacteria; (3) obtaining indigenous bacteria by collection, culture and enrichment culture; (4) performing domestication culture of working bacteria, namely adding indigenous bacteria and a composite culture medium according to different sludge loads, adding bacillus subtilis enzyme powder in an amount which is 0.1 to 0.4 percent based on total weight and bacterial solution of photosynthetic bacteria in an amount which is 1 to 3 percent based on the total weight, performing tests under different loads and different organic matter concentration, determining an optimal parameter, and repeating for 3 times; and (5) performing domestication and amplification culture of the working bacterium composite agent, and obtaining the domesticated working bacterium composite agent in which the viable count per milliliter is cfu 10<7> to 10<9>. The enzyme and bacterium composite agent prepared by the method can treat sewage and sludge efficiency, simplyand conveniently, has very high adaptability to any sewage environment, and has a guiding and coordinating effect on beneficial decomposing bacteria in local environments.

Description

technical field [0001] The invention relates to a preparation method of an enzyme-bacteria compound agent for treating sewage sludge (silt). The enzyme-bacteria compound agent can be used to treat urban domestic sewage, livestock and poultry breeding wastewater, biogas slurry, river bends, lakes, ponds, swamps, Landscape and other sewage sludge in closed or semi-closed waters where the water flow is stagnant or the water flow is very slow, and it belongs to the technical field of environmental protection. Background technique [0002] The water environment is an important part of the earth's ecological environment. With the development of the economy, water pollution is also a problem that cannot be ignored. Due to the concentration of industry and commerce and dense population in my country's cities, the production of domestic sewage is large, and there are many types of pollutants and high loads. 80% of urban sewage is domestic sewage, mainly feces, rot and other sewage, ...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N9/00C12R1/01C12R1/125
Inventor 赵凤清王冬柴宝荣
Owner 康源绿洲微生物技术(北京)有限公司
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