1289 results about "Enrichment culture" patented technology

A method for inexpensive and efficient PHA biosynthesis includes operating a sequencing bioreactor in alternating phases of nutrient deprivation and carbon feedstock deprivation to select for robust PHA-producing microbes. Preferably, the bioreactor is operated in a non-sterile manner with mixed cultures of methanotrophs. The method also preferably uses periodic biomass-wasting (PHA harvesting) at the end of the carbon feed phase, gradually lengthening the time period of carbon deprivation phase to create a penalty for rapid PHA degradation and incentive for PHA accumulation. Also, bacterial enrichment cultures may be introduced periodically. The PHA-accumulating bacteria are preferably grown on common anaerobic degradation products, specifically volatile fatty acids, such as acetate and propionate, and methane gas. The PHA has useful applications in bioplastics and other products.

The invention discloses a large-scaled culture method of high-concentration nitrosobacteria and application thereof. The method comprises the following steps of: (1) adding an enrichment culture solution with initial ammonia nitrogen mass concentration of 30-500mg / L in a reaction device with functions of stirring, heating and aeration; preparing activated sludge of a waste water treatment plant in activated sludge of 0.5-20% for primary inoculation to conduct the enrichment culture of the nitrosobacteria by 2-6d as a culture cycle, wherein extra ammonium salts and a growth promoter are added during the enrichment culture of the nitrosobacteria; (2) ending the culture when the concentration of NO2-N in the solution is accumulated to reach 70-95% of the concentration of total nitrogen; adding a flocculating agent for flocculation and sedimentation; and allowing to stand still to remove supernate, and then adding the enrichment culture solution to start the culture of the second cycle; and (3) continuously conducting 3-8 cycles of the enrichment culture to obtain the high-concentration nitrosobacteria. By adopting the nitrosobacteria to strengthen the bi-nitrification treatment of ammonia nitrogen contained waste water, the method has the advantages of high stress resistance, low cost and high efficiency and is suitable for the complicated treatment of ammonia nitrogen contained industrial waste water.

The invention belongs to the technical field of microalga biology, and particularly relates to a microalga strain with high CO2 tolerance and high fixation rate and a breeding method thereof. The microalga is named Chlorella sp.Y-1, and is collected into Common Microorganism Center of China General Microbiological Culture Collection Committee; and collection number is CGMCC No.5429. The invention has the following characteristics: the initial strain is separated from natural environment; after high-CO2-concentration enrichment culture, gene cloning, induced mutation breeding and other operations, the carbon content of the target strain is increased to 56.981%, the optimal fixation concentration is up to 20% (v / v), the fixation rate is up to 5.762g / (L.d), and the maximum tolerance concentration is 100% (v / v); and the target strain has high adaptability to a series of physical and chemical culture conditions, has high culture transfer stability, and high better overall properties than the like research findings at present. The invention aims to solve the bottleneck problem in high concentration CO2 biological fixation technology, cultures an efficient organism capable of efficiently fixing high concentration CO2, implements effective fixation of CO2 in high concentration flue gas and other complex environments, and meanwhile, generates beneficial biological substances.

The invention discloses a enrichment method of nitrifying organism. The method adapts discontinuous activated sludge process by gradually improving ammonia nitrogen ph indicator in nutrient fluid to enrich. The main component of the enrichment nutrient fluid is inorganic salt comprising microelement Fe, Mg, Na, K and amortization liquor, in which inceptive concentration of NH(4)(+-N) is 100mg / L to 200mg / L, final concentration is 500mg / L to 1200mg / L and COD is less than or equal to 200mg / L. The method can restrain evidence the growth of sundry bacterium such as carbonizing bacterium, is propitious for nitrifying organism to become ascendency bacterium and resist more and more high ammonia nitrogen concentration, and finally disposing concentration of the ammonia nitrogen wasted water is up to 1200mg / L and high concentration ammonia nitrogen in wasted water can be reduce to less 10mg / L.

The invention discloses a preparation method and an application of immobilized bacteria for improving the denitrification efficiency of a constructed wetland. 1. The preparation steps are as follows: 1) screening and culturing aerobic denitrification strains which are screened out of the constructed wetland in a thermotank; 2) selecting aerobic denitrification bacteria in slope conservation, inoculating into a sterile enrichment culture medium and aerating to get bacterial suspension; 3) using sodium alginate-polyvinyl alcohol to embed so as to get the immobilized aerobic denitrification bacteria; and 4) taking pellets of the aerobic denitrification bacteria out of a refrigerator, washing with double distilled water or physiological saline, soaking in the physiological saline, and aerating to activate the immobilized pellets. 2. An application of the immobilized bacteria in the constructed wetland is as follows: filling the immobilized pellets into a simulation column of the constructed wetland for performing waste water denitrification treatment. The preparation method of the immobilized bacteria is simple, the production cost is low, the application in the industrial waste water treatment is easy, the denitrification efficiency is high, the accumulation amount of nitrite nitrogen is low, and the service life is long.

The invention relates to a method for preparing an enzyme and bacterium composite agent for treating sewage and sludge, which comprises the following steps: (1) preparing bacillus subtilis powder; (2) preparing bacterial solution of photosynthetic bacteria; (3) obtaining indigenous bacteria by collection, culture and enrichment culture; (4) performing domestication culture of working bacteria, namely adding indigenous bacteria and a composite culture medium according to different sludge loads, adding bacillus subtilis enzyme powder in an amount which is 0.1 to 0.4 percent based on total weight and bacterial solution of photosynthetic bacteria in an amount which is 1 to 3 percent based on the total weight, performing tests under different loads and different organic matter concentration, determining an optimal parameter, and repeating for 3 times; and (5) performing domestication and amplification culture of the working bacterium composite agent, and obtaining the domesticated working bacterium composite agent in which the viable count per milliliter is cfu 10<7> to 10<9>. The enzyme and bacterium composite agent prepared by the method can treat sewage and sludge efficiency, simplyand conveniently, has very high adaptability to any sewage environment, and has a guiding and coordinating effect on beneficial decomposing bacteria in local environments.

The invention discloses a method for preparing and using a microbial immobilized material for repairing contaminated soil. The method comprises the following steps: (1) after being dried by air, the waste biomass raw material is pulverized or ground into 1-20mm of particles; (2) the biomass particles are carbonized and sterilized at the temperature of 150-450 DEG C and under limited oxygen condition for 0.5-6 hours to prepare a carrier material; (3) the carrier material is put into bacterium suspension for enrichment culture according to the solid-to-liquid ratio of 0.5-2mL / g, then the microbial immobilized material is produced; (4) the produced microbial immobilized material is added to organic contaminated soil according to the proportion of 1-500 tons per hectare soil, 0-30cm of topsoil is ploughed to cause the microbial immobilized material to be mixed evenly in the soil, simultaneously, the contaminated soil is biologically repaired and safe agricultural products are produced. The method has the advantages that the carrier material contains abundant lignin structures, carbon and nutrient elements, which provides more suitable matrix for microbial growth; and the immobilized material has strong affinity, high immobilization efficiency, strong adsorption capacity and good resistance against the pollutants in the environment; and the immobilized material is a soil fertilizer, and is suitable for large-scale in-situ biological repair of the contaminated soil.

The invention relates to a loach fry artificial breeding method which is characterized by including the steps of selecting healthy parental loaches above two winters old and conducting enrichment culture for 25-35 days; then conducting hybrid injection of HCG (human chorionic gonadotropin) and DOM (domperidone) for artificially hastening parturition; after 10-14 hours after parturition hastening, collecting fertilized eggs; and then conducting artificial incubation and larva breeding, thus obtaining the loach fry. In the invention, through the enrichment culture of the parental loaches, synchronous maturing is promoted and oogonium percentage is enhanced; with the parental loach parturition hastening technology, synchronous egg laying can be realized; with the hybrid injection method, the ovarian development and the egg laying effect of the loaches can be promoted, so that the synchronous egg laying rate of the parental loaches achieves 40%; and an inflation incubation way is adopted on the fertilized eggs, so that the incubation rate of the fertilized eggs can be greatly improved. Through enrichment culture, the survival rate of the loach fry can be enhanced remarkably.

The invention relates to the technical field of plant stem cell culture, particularly an apple stem cell culture method and apple stem cells cultured by the method. The method comprises the following steps: by using an apple new branch as a raw material, inducing to form callus, and carrying out enrichment culture and amplification culture to obtain the apple stem cells. The method provided by the invention has the advantages of shorter period and high yield. The method can obtain abundant apple stem cells within about 48 days. The induction culture medium used in the apple stem cell culture process and the variety of the phytohormones contained in the enrichment culture medium are proper and suitable in proportion, and can ensure fast dedifferentiation and abundant reproduction of apple branch cells. The apple stem cells can be freeze-dried or extracted and used for preparing food, drugs, health products or cosmetics. The apple stem cell extraction method provided by the invention is simple and easy to implement and has higher extraction efficiency. The prepared extract contains abundant flavones and polyphenols.

The invention discloses an enrichment culture method of salt-tolerant nitrifying bacterium communities, which comprises the steps that: activated sludge is inoculated, and the culture mode is the sequencing batch type enrichment culture; the enrichment culture is carried out in simulated wastewater, the main ingredient of nutritive salt of the enrichment culture is inorganic salt, the substrate is externally added ammonium salt, and a carbon source is inorganic carbonate; the pH value in an aeration tank in the enrichment culture process is regulated and controlled through sodium carbonate and sodium bicarbonate, and the dissolved oxygen value is regulated through the aeration quantity; a method for gradually increasing the salinity is adopted for domesticating the nitrifying bacterium communities; and when no filamentous bacterium is observed in the sludge and the number and the variety of protozoans are few, the enrichment culture of the nitrifying bacterium communities is finished. The enrichment culture method has the advantages that the available matrix range is wide, the culture is easy, the concentration of the cultured salt-tolerant nitrifying bacterium communities is high, the activity is good, the biochemical treatment removal rate of high-salt ammonia-nitrogen wastewater can be obviously improved, the enrichment culture method is suitable for the treatment of various ammonia-nitrogen industrial wastewater and is suitable for the scale production, the wastewater treatment cost is reduced, and good economic benefits and environmental benefits are realized.

A rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua relates to the tissue culture technology of plants. The formulas of culture mediums are as follows: an induction culture medium includes MS, 1.0 mg / L of 6-BA, and 0.5 mg / L of NAA, a proliferation culture medium for root tubers with buds include MS, 10 mg / L of 6-BA, 0.5 mg / L of NAA, and 60 g / L of sugar, and a proliferation culture medium for the bud body parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of 2,4-D, and 60 g / L of sugar, a proliferation and seedling strengthening culture medium for the root tuber parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of NAA, 0 to 50 mg / L of paclobutrazol, and 60 g / L of sugar, and a rooting culture medium for the root tuber parts include MS, 0.5 mg / L of 6-BA, 0.5 mg / L of NAA, and 0.5% of activated charcoal. The method comprises the steps as follows: the surface layers of the root tuber parts of the disinfected root tubers with buds are removed, the root tubers with buds are cut into root tubers and buds after being cultivated in the induction culture medium and the proliferation culture medium, the root tubers are transferred to enrichment culture and cultivated circularly for 3 to 6 generations, the buds are transferred to proliferation culture of the bud parts and are proliferated in a compound and circulating manner for 3 to 6 generations, and proliferation strong seedling culture and rooting culture are performed to final end products which are the root tubers with buds. The method is applicable to rapid propagation and scale production.

The invention relates to a tissue culture and rapid propagation method of acer palmatum. The method comprises the following steps of: after a series of sterilization processes, carrying out isolated culture on acer palmatum stems by adopting NN69 as a basic culture medium; and establishing a rapid propagation system of the acer palmatum by utilizing hormone regulation and control to successfully culture an integrated rooting regeneration plant with a transplant survival rate of about 90 percent, wherein a starting culture medium for inoculating a primary generation comprises the NN69, 0.1mg / L of NAA (Naphthylacetic Acid), 0.3mg / L of TDZ (Thidiazuron) and 30g / L of sucrose with a pH value of 5.8; an enrichment culture medium comprises the NN69, 30g / L of sucrose, 0.1mg / L of NAA and 0.8mg / L of KT-30; and a rooting culture medium comprises 1 / 2 NN69, 0.2mg / L of NAA, 20g / L of sucrose and 0.2g / L of active carbon. The method overcomes the defects of long conventional seedling culturing period of the acer palmatum, low cuttage propagation coefficient, and the like and realizes the industrial production of acer palmatum nursery stocks.

Products and methods for controlling animal wastes are disclosed. The methods include making an inoculum containing a mixture of Bacillus species; mixing the inoculum with animal wastes and then incubating the mixture to result in an enriched culture; and then applying the enriched culture to the animal wastes. The inoculum for controlling wastes includes a mixture of Bacillus species in a liquid medium in which the mixture of Bacillus species were incubated.

The invention relates to a method for remedying acidified arsenic contaminated soil by biochar-loaded nano-scale zero-valent iron cooperated with bacteria. The method includes selecting Pseudomonas putida strain MnB1 (ATCC23483); carrying out enrichment culture on the Pseudomonas putida strain in enrichment culture media; inoculating strains in culture media with divalent manganese and carrying out culture on the strains to obtain active metabolites; adding the active metabolites and the green synthetic biochar-loaded nano-scale zero-valent iron into the acidified arsenic contaminated soil anduniformly stirring the active metabolites, the biochar-loaded nano-scale zero-valent iron and the acidified arsenic contaminated soil; carrying out a series of physical-chemical reaction on active manganese oxide, zero-valent iron, biochar and trivalent arsenic or pentavalent arsenic in the soil; converting the arsenic in exchangeable forms into arsenic in residual forms. The method has the advantages that the arsenic in the soil can be effectively immobilized, the pH (potential of hydrogen) of the soil can be increased, and the double purposes of remedying soil acidification and arsenic contamination can be simultaneously achieved; the method is short in remediation time, high in efficiency, wide in treatment range and free of secondary pollution, and stable effects can be realized.

The invention relates to an anaerobic ammonia oxidized coupled heterotrophic denitrifying denitrification method and belongs to the technical field of biological sewage treatment. The method includes adopting anaerobic ammonia oxidized granule sludge as the inoculated sludge; manually distributing water to add ammonium chloride, sodium nitrite, and phenol which is taken as organic matter, realizing enrichment culture of anaerobic ammonium oxidized bacteria and heterotrophic denitrifying bacteria, determining competitive and cooperative relations of the anaerobic ammonium oxidized bacteria and heterotrophic denitrifying bacteria, and realizing the enrichment culture of mixed flora of the anaerobic ammonium oxidized bacteria and heterotrophic denitrifying bacteria after a period of time.

The invention provides a preparation method of a kimchi fermenting agent and a fermenting bacterium powder. The preparation method comprises the steps: activating strains of lactobacillus plantarum, lactobacillus brevis, lactobacillus pentosus and lactobacillus paracasei; preparing an enrichment medium; carrying out enrichment culture on the activated strains; centrifugally concentrating the kimchi fermenting agent; preparing a protective agent; and carrying out spray drying on the kimchi fermenting agent and the protective agent. The kimchi fermenting agent and the fermenting bacterium powder prepared by using the method can be used for effectively reducing nitrite in kimchi; a vegetable generally used as kimchi is selected as the enrichment medium, so that not only can lactic acid bacteria be effectively proliferated, but also the enrichment medium has the characteristics of natural green and no addition of other chemical reagents; a spray drying method is used as a drying way for preparing the fermenting bacterium powder, so that the production cost can be effectively reduced.

The invention relates to immobilized microspheres for remediation of petroleum contaminated soil, a preparation method thereof and applications thereof. The immobilized microspheres are spheroidal, have internal apertures and have diameters of from 2.5 to 3.0 millimeters. The immobilized microspheres comprise sodium alginate as an embedding material and attapulgite as an adsorbent for adsorptions of petroleum hydrocarbon degrading bacteria and nutrient elements. The preparation method of the immobilized microspheres comprises embedding petroleum hydrocarbon degrading bacteria and nutrient elements through sodium alginate as an embedding material and attapulgite as an adsorbent, and carrying out a series of preparation steps of high density bacteria liquid suspension, immobilization and balling, and enrichment culture to obtain the immobilized microspheres. The immobilized microspheres prepared through the preparation method provided by the invention have favorable aperture conditions and adsorption performances, thereby having strong load capabilities. The immobilized microspheres provide good internal survival environments for petroleum hydrocarbon degrading bacteria, thereby having strong environmental impact resistance capabilities. The immobilized microspheres have the advantages of environmental protection, safety, good biocompatibility and wide foreground of application in bioremediation for petroleum contaminated soil.

The invention discloses a biochemical treatment method for wastewater with high ammonia nitrogen concentration. The method is as follows: nitrobacterium-enriched activated sludge is cultured in a nitrified sludge enrichment tank at first and then is led into a nitration treatment tank containing wastewater with high ammonia nitrogen content and low COD, and sewage after nitration treatment enters into a denitrification tank for nitrification and denitrogenation treatment, wherein, the process for culturing the nitrobacterium-enriched activated sludge adopts the intermittent activated sludge process and enrichment is performed by gradual improvement of the concentration of ammonia nitrogen in culture solution; the used enrichment culture solution comprises microelements including ferrum, magnesium, sodium and potassium and buffer solution, and particularly also comprises Ca<2+> with a concentration between 0.01 and 0.05 gram per liter; the final concentration of the ammonia nitrogen during the culture process is between 300 and 1200 micrograms per liter; and the COD is less than or equal to 200 micrograms per liter. The method can effectively process the wastewater with high ammonia nitrogen content and low COD value, and the processing method is simple and low in cost.

The invention discloses a strain which can produce amber acid and its sieve method and its application. The said strain is named by classification as Actinobacillus succinogenes NJ113 and has been preserved in China microbial bacterial preservation management committee common microorganism center with CGMCC No.1716 as its preserved number. The invention prepares the ray bacillus which can produce amber acid with a high yield by enrichment culture of strain in animal cud in culture medium containing disodium fumarate and the following classification. The amber acid with higher concentration can be produced by performing fermentation by using the said strain, which is suitable for the commercial manufacture of amber acid.

The invention discloses a method for culturing granular sludge by shortcut denitrification. The method comprises the following steps of putting inoculated sludge into an aerobic reactor, and performing enrichment culture on denitrifying sludge by using high ammonia-nitrogen low COD wastewater; then, performing secondary enrichment screening by wastewater simultaneously containing nitrate nitrogen, nitrite and COD in a facultative anaeration reactor, and performing third enrichment screening by wastewater only containing nitrite and COD; and finally acclimating by wastewater simultaneously containing ammonia-nitrogen, nitrite and COD to obtain the denitrified granular sludge with high removal rate on the nitrite when various forms of nitrogen coexist commonly. By the method, the denitrified granular sludge which can survive in a complicated environment and is capable of effectively treating wastewater nitrous nitrogen under proper condition can be obtained, and the shortcut nitrification-denitrification process can be truly applied to actual engineering conveniently.

The invention discloses a method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation. The method comprises the following steps: adopting fiber and sponge combined ball-type packings which are independently developed as carriers to perform screening, placing high effective strain which is obtained through screening in culture solution 'HS enrichment medium' to perform enrichment culture, then inoculating the strain which is through the enrichment culture in 'HSK culture medium' to perform expending cultivation and prepare biological microbial inoculum, placing packings in the microbial inoculum to perform biofilm formation and obtain biological immobilized packings; connecting the prepared biological immobilized packings with a string to fix the packings around the laboratory patented device water-lifting aerator of the invention and adopting water-lifting aeration-situ bio-contact oxidation to perform bioremediation to the micro-scale polluted reservoir water. The method of the invention can be used to effectively reduce the concentrations of COD, N, P and other pollutants in reservoir water and especially effectively lower the total nitrogen content of the micro-scale polluted reservoir water so as to fundamentally resolve the pollution problem of reservoir water, inhibit the growth of algae and control water eutrophication.

The invention discloses a composite bacterial agent for efficiently degrading kitchen garbage, and a preparation method and application thereof. The efficiently degrading kitchen garbage, and preparation method and application thereof consists of a starch-degrading strain, an oil-degrading strain, a protein-degrading strain, a cellulose-degrading strain and a vector; and the preparation method comprises the following steps of: performing enrichment culture on each single strain, screening, and preparing the vector; and mixing a high-quality composite bacterial agent with the vector. The synergism of various microorganisms in the natural world is fully utilized, a microorganism-based control method is established, and the composite bacterial agent is utilized for coupled fermentation of the kitchen garbage to accelerate the biogas fermentation progress, improve the traditional fermentation process and improve the biogas yield.

The invention provides a method and a device for quickly enriching ammonia oxidizing bacteria. The ammonia oxidizing bacteria are enriched by a sequencing batch reactor, a method of gradually increasing ammonia nitrogen concentration in a culture solution and a pH process controlling method; and the adopted culture solution for enriching comprises the inorganic salt used as a main component, and a trace element culture solution, a buffer solution and ammonia carbonate; and the concentration of dissolved oxygen in the culture process is maintained more than 5mg / L by a process controlling device, pH is maintained 7.0 to 8.5, and temperature is maintained 20 to 25 DEG C. By the scheme, the growth of heterotrophic bacteria in activated sludge can be inhibited obviously, and the enriched ammonia oxidizing bacteria are 60 to 65 percent of the total number of microorganism bacteria in the activated sludge, and are tolerant to high ammonia nitrogen concentration; and finally high-ammonia-nitrogen wastewater of which the concentration can reach 500mg / L can be treated, and high-concentration ammonia nitrogen in the wastewater can be reduced to less than 1.0mg / L.

The invention discloses a strain producing succinic acid, a screening method and application thereof. The strain is classified as Actinobacillus succinogenes NJ113, and has been preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No.1716. In the invention, the bacterial strains in the animal rumen are enriched and cultivated in the medium containing disodium fumarate and then classified to obtain actinomycetes with high production of succinic acid. Fermentation by using the bacterial strain can produce succinic acid with higher concentration, which is suitable for industrial production of succinic acid.

The invention discloses a porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine and a preparation method thereof. The preparation method comprises the following steps of: a, respectively carrying out enrichment culture on a porcine streptococcus strain, a haemophilus parasuis strain and a haemophilus parasuis strain to obtain a porcine streptococcus strain bacterial solution, a haemophilus parasuis strain bacterial solution and a haemophilus parasuis strain bacterial solution; b, respectively adding a formaldehyde solution into the porcine streptococcus strain bacterial solution, the haemophilus parasuis strain bacterial solution and the haemophilus parasuis strain bacterial solution, and inactivating; c, mixing the collected porcine streptococcus strain bacterial solution, the haemophilus parasuis strain bacterial solution and the haemophilus parasuis strain bacterial solution, adding Tween-80 for preparing a water phase, preparing white oil, Span-80 and aluminium stearate into an oil phase, mixing the water phase with the oil phase to prepare a uniform emulsion, i.e. an oil emulsion inactivating vaccine; and 4, sub-packaging the oil emulsion inactivating vaccine. The porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine can effectively prevent the porcine streptococcus disease and haemophilus parasuis disease, does not have hidden danger of scattering viruses and is safe and reliable; and the immunization is realized by one vaccine, thus the cost is reduced.

The invention provides a culture method of salt-tolerant anaerobic ammonium oxidation bacteria, which comprises the following steps of: A, enrichment culture: performing enrichment culture on the strain to obtain anaerobic ammonium oxidation bacteria; and B, bacteria acclimation culture: gradually increasing the concentration of salt in the culture solution to perform salt tolerance acclimation culture of the anaerobic ammonium oxidation bacteria subjected to the enrichment culture in the step A, wherein the acclimation period of the bacterial at each salt concentration is 15-30 days; and the step A and step B both adopt sequencing batch culture, and the dissolved oxygen value in the culture solution is 0-0.5mg / L. The bacteria cultured by the method provided by the invention are applied to high-salt ammonia nitrogen wastewater, and the total nitrogen removal rate is high. By adopting the method provided by the invention to culture the salt-tolerant anaerobic ammonium oxidation bacteria, the range of available substrates is wide, and the culture is easy to implement; and additional organic carbon source is not required in the bacteria culture and application process, investment is greatly saved, and the wastewater treatment cost is reduced. The bacteria culture method provided by the invention can be applied to large-scale production, and has good economic and environmental benefits.

The invention discloses an immobilized microbial preparation which is prepared through the following steps: S1, fermentation culturing strain: respectively inoculating bacillus subtilis, bacillus megatherium, nitrobacter and nitrosomonas in an enrichment medium to be cultured; S2, mixing thallus to obtain mixed thallus liquor; S3, immobilizing PVA-sodium alginate, namely uniformly mixing PVA gel with the mixed thallus liquor, dripping the mixture into a boric acid solution containing CaCl2 while continuously stirring at a low speed, immobilizing and crosslinking the mixture, taking out the mixture, washing the mixture through deionized water, and freeze drying the mixture to obtain the immobilized microbial preparation. The invention further discloses a method of treating meat product processing waste water by utilizing the immobilized microbial preparation. The immobilized microbial preparation disclosed by the invention has the beneficial effects that the microorganism categories are various, various pollutants in the waste water can be degraded, the degrading speed is fast and the capability is strong; the immobilized microbial preparation is suitable for changes of water quality and pH; the sludge yield is small, the products are liable to separate, the stability is good, the technique operation is simple, and the production cost is low.

The invention discloses a method for constructing strain Escherichia coli JM127 in high product malic acid gene project and acid producing method thereof. The constructing method mainly includes deactivating or knocking out enzyme with ability to make malic acid dehydrate and pyruvate degrade, and over-expressing enzyme regenerated by NAD. A two-stage fermentation experiment using a constructed Escherichia coli is carried out, which includes steps of: (1) inoculating activated Escherichia coli into enrichment medium, aerobically culturing to enhance biomass; (2) after treating the bacteria liquid membrane obtained by aerobic culture, introducing it into LB culture medium containing 20-100 g / L glucose and a definite content of carbonate, anaerobically fermenting to produce malic acid.