A rapid propagation method for tube seedlings of high-grade
polygonatum cyrtonema hua relates to the
tissue culture technology of plants. The formulas of
culture mediums are as follows: an induction culture medium includes MS, 1.0 mg / L of 6-BA, and 0.5 mg / L of NAA, a proliferation culture medium for root tubers with buds include MS, 10 mg / L of 6-BA, 0.5 mg / L of NAA, and 60 g / L of
sugar, and a proliferation culture medium for the
bud body parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of 2,4-D, and 60 g / L of
sugar, a proliferation and
seedling strengthening culture medium for the root tuber parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of NAA, 0 to 50 mg / L of
paclobutrazol, and 60 g / L of
sugar, and a rooting culture medium for the root tuber parts include MS, 0.5 mg / L of 6-BA, 0.5 mg / L of NAA, and 0.5% of
activated charcoal. The method comprises the steps as follows: the surface
layers of the root tuber parts of the disinfected root tubers with buds are removed, the root tubers with buds are
cut into root tubers and buds after being cultivated in the induction culture medium and the proliferation culture medium, the root tubers are transferred to
enrichment culture and cultivated circularly for 3 to 6 generations, the buds are transferred to proliferation culture of the
bud parts and are proliferated in a compound and circulating manner for 3 to 6 generations, and proliferation strong
seedling culture and rooting culture are performed to final end products which are the root tubers with buds. The method is applicable to rapid propagation and scale production.