Rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua

Abstract

A rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua relates to the tissue culture technology of plants. The formulas of culture mediums are as follows: an induction culture medium includes MS, 1.0 mg / L of 6-BA, and 0.5 mg / L of NAA, a proliferation culture medium for root tubers with buds include MS, 10 mg / L of 6-BA, 0.5 mg / L of NAA, and 60 g / L of sugar, and a proliferation culture medium for the bud body parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of 2,4-D, and 60 g / L of sugar, a proliferation and seedling strengthening culture medium for the root tuber parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of NAA, 0 to 50 mg / L of paclobutrazol, and 60 g / L of sugar, and a rooting culture medium for the root tuber parts include MS, 0.5 mg / L of 6-BA, 0.5 mg / L of NAA, and 0.5% of activated charcoal. The method comprises the steps as follows: the surface layers of the root tuber parts of the disinfected root tubers with buds are removed, the root tubers with buds are cut into root tubers and buds after being cultivated in the induction culture medium and the proliferation culture medium, the root tubers are transferred to enrichment culture and cultivated circularly for 3 to 6 generations, the buds are transferred to proliferation culture of the bud parts and are proliferated in a compound and circulating manner for 3 to 6 generations, and proliferation strong seedling culture and rooting culture are performed to final end products which are the root tubers with buds. The method is applicable to rapid propagation and scale production.

Description

Technical field:

[0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapid propagation of high-quality Polygonatum multiflora test-tube seedlings. Background technique:

[0002] Polygonatum cyrtonema Hua (Polygonatum cyrtonema Hua) is a medicinal plant of the genus Polygonatum cyrtonema. Its rhizome is used as a traditional Chinese medicine Polygonatum cyrtonema Hua. It contains polysaccharides, amino acids, anthraquinones and other components. It has antibacterial, antihypertensive, anti-aging and treatment of rheumatism. . It is cultivated in some areas in southern my country, and it mainly relies on the asexual reproduction of rhizomes and seeds for reproduction. However, due to the low reproduction coefficient and the slow speed of the seed reproduction cycle, a large number of seedlings cannot be provided, so large-scale planting is limited. In addition, long-term asexual reproduction can easily lead to the degrada...

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