12 results about "Asexual reproduction" patented technology

This invention discloses the agronomic traits, InDel-labeled fingerprint, and construction method of the *Dictyophora indicum* strain YZS021, belonging to the field of *Dictyophora indicum* detection technology. The fingerprint is obtained by PCR amplification and electrophoresis detection using 10 pairs of InDel-labeled primers developed based on the whole genome of *Dictyophora indicum*. The InDel marker fingerprint spectrum detection of the *Dictyophora indicum* strain YZS021 of this invention is fast, accurate, and reproducible, and can specifically identify the strain, enabling precise identification of the fungal species and protection of intellectual property rights. In addition, this invention also discloses the agronomic traits of the *Dictyophora indicum* strain YZS021. The *Dictyophora indicum* strain 'YZS021' has a long and thick stipe, a high edible ratio, and good commercial characteristics. It can stably produce fruiting under both forest and facility cultivation modes, adapting to different cultivation scenarios and significantly improving planting efficiency. The above agronomic traits have consistency and stability, and can stably cultivate and obtain asexual reproduction lines with the above traits in both forest and facility fruiting rooms.

This invention relates to a rooting agent and seedling cultivation method for promoting rooting of *Cyclocarya paliurus* cuttings, belonging to the field of plant asexual reproduction technology. It solves one of the problems in existing *Cyclocarya paliurus* seedling cultivation: low rooting rate, long rooting time, few and poor-quality adventitious roots, and low transplant survival rate. This invention discloses a rooting agent for promoting rooting of *Cyclocarya paliurus* cuttings, which consists of two components, A and B. Component A consists of potassium indolebutyrate, soluble starch, chlorogenic acid, salicylic acid, and talc; component B consists of indolebutyric acid, sucrose, chlorogenic acid, vitamin C, and talc. Using the above-mentioned rooting agent and the corresponding seedling cultivation method, rapid rooting of *Cyclocarya paliurus* cuttings is achieved, with a rooting rate as high as 93.06%, and a large number and good quality of adventitious roots, resulting in a transplant survival rate of 90.37%.

This invention discloses a species of Armillaria mellea ZM51, whose classification name is [not specified]. French Armillaria This strain was deposited on May 8, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.42001, located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. *Armillaria galbana* ZM51 exhibits unique characteristics such as rapid mycelial germination, strong resistance to contamination, and early fruiting. Through multiple generations of asexual reproduction, its traits remain stable and consistent. Compared to the existing *Armillaria olfleurone* 'Hazelnut-4', its mycelial germination time is shortened by 3-6 days, and fruiting occurs approximately 20-25 days earlier. In Liaoning province, through semi-wild cultivation, it achieves autumn fruiting after spring sowing, with clustered fruiting bodies, significantly larger mushrooms than 'Hazelnut-4', thicker stipes, and larger caps, demonstrating significantly enhanced resistance to contamination.

The present application relates to the technical field of plant asexual reproduction, and particularly discloses a cutting propagation method for Lindleya benzoe. The method comprises the following steps: pretreating the base of cuttings with an activating solution containing oligosaccharide signal molecules and active components of root exudates of Lindleya benzoe, then cutting the cuttings in a special induction matrix containing slow-release plant growth regulators, and performing programmed induction of dark culture and light culture; during the light culture stage, dilute solution of the activating solution is applied to the base of the cuttings as an auxiliary inducer. The present application integrates endogenous signal activation, slow-release continuous supply and phased synergistic induction, systematically simulates and strengthens the chemical biological microenvironment of natural rooting, effectively activates the endogenous root potential of the cuttings, and solves the technical problems of low rooting rate, poor root quality and weak transplanting adaptability in conventional methods, thereby realizing efficient and high-quality propagation of Lindleya benzoe cutting seedlings.

The application discloses a jellyfish polyploid induction method and application thereof, and belongs to the technical field of marine ornamental animal breeding. The jellyfish polyploid induction method takes the polyp stage of jellyfish as an induction object, and polyploid jellyfish is obtained by induction treatment through combination of colchicine and dimethyl sulfoxide. The method can obtain multiple polyploid new germplasms through one treatment under the use of a small amount of inducers. The obtained polyploid polyps can not only rapidly proliferate new individuals with polyploid characteristics through asexual reproduction, but also obtain jellyfish bodies with polyploid characteristics, such as fast growth speed, long life cycle, beautiful shape and large individual. Meanwhile, the method has the characteristics of simple operation and low cost. Therefore, the induction method has great application value and broad application prospect.

This invention belongs to the field of crop genetics and breeding technology, and particularly relates to a method for breeding large-fruited thornless prickly pears using thornless prickly pear germplasm resources. The method includes the following steps: screening key parents from the seedling progeny population of thornless prickly pear germplasm, from which individuals exhibiting the thornless trait can be isolated; germinating the seeds of the key parents' progeny until they show white sprouts, and then chemically mutagenesis using colchicine solution to obtain a first-generation mutant population; selecting mutant plants from the first-generation mutant population with completely thornless fruit and a single fruit weight of not less than 20 grams; and performing asexual reproduction on the mutant plants, verifying through multiple generations of cultivation to obtain varieties with stable thornless and large-fruit traits. This invention effectively solves the technical problem of small fruit size and difficulty in genetically fixing the thornless trait in breeding from thornless prickly pear germplasm resources, creating a new prickly pear germplasm with large fruit size, thornlessness, and stable traits.

The present application relates to the field of asexual reproduction technology, and more particularly to a method for large-scale cultivation of seedlings by using pineapple waste stem in field. The method comprises the following steps: after harvesting of pineapple fruits, collecting healthy above-ground stems of pineapple, removing leaves and residual fruit stalks, and soaking the base of the stems in a composite pretreatment solution for 25-35 minutes; immersing the pretreated stems in an indole butyric acid solution for 8-15 seconds; ridging, ditching on the ridge surface, and inserting the stems into the ditch at an angle of 35-45 degrees with the bud eye facing upwards, with 2 / 3 of the length of the stems being buried in the soil and the remaining part being exposed to the ground; pouring water thoroughly after insertion, controlling the light intensity, maintaining the soil temperature at 20-30 DEG C, and culturing for 45-60 days; when the newly grown seedlings grow to 15-25 cm, gradually increasing the light intensity for 7-10 days of seedling hardening, and taking out the stems together with the base when the seedlings are transplanted, and obtaining the seedlings after separation. The present application converts waste stems into seedlings, and realizes in-situ resource utilization of waste.

This invention discloses a convenient and rapid method for propagating kudzu seedlings from leaves. By employing specially designed cutting plastic devices one and two, combined with the method of this invention, the growth status of the seedlings can be monitored in a timely manner, and nutrients can be provided. This significantly shortens the seedling time (approximately 10 days faster than traditional cutting propagation) and effectively improves the survival rate of kudzu seedlings (far exceeding the traditional cutting survival rate, reaching 90.0%) and the efficiency of seedling cultivation, while reducing the cost of kudzu seedling cultivation. Furthermore, this invention utilizes asexual reproduction, producing kudzu plants of consistent quality with the parent plant.

This invention discloses a specific DNA fragment, primers, identification method, and application for sex identification in yellowfin tuna. The specific DNA fragment has the following gene sequence: male as shown in SEQ ID NO.1 and female as shown in SEQ ID NO.2. Primers were designed based on the specific DNA fragment sequence for PCR amplification and Sanger sequencing analysis. The genotype at position 219 of the male yellowfin tuna DNA fragment is T / G heterozygous, and at position 292 it is A / G heterozygous. The genotype at position 219 of the female yellowfin tuna DNA fragment is T / T homozygous, and at position 292 it is A / A homozygous. This invention successfully achieves non-lethal sex identification during the fry or juvenile stage, which is beneficial for the implementation of asexual reproduction and seedling selection in yellowfin tuna, and has significant practical value and application potential.

This invention provides a method for establishing a rapid propagation system of *Iris tectorum* through tissue culture using autumn dormant buds. The method uses autumn dormant buds of *Iris tectorum* as explants. Aseptic buds are obtained by removing the base and excess above-ground parts, fixing, peeling, and clamping. The buds are then longitudinally cut to increase the propagation coefficient, inducing clustered lateral buds, and then inducing rooting to form aseptic mature plants. The aseptic rate after 7 days is 57.12%, and the fungal rate is 0.15%. The aseptic rate after 20 days of the first subculture is 84.66%, and the rooting rate after 50 days is 63.33%. This method eliminates the need for callus induction and differentiation stages, significantly shortening and simplifying the time and procedure for obtaining aseptic *Iris tectorum* seedlings. The method is easy to control, low in cost, and highly efficient. It provides a new approach for further subculture or callus redifferentiation to establish a highly efficient rapid propagation system for monocotyledonous plants, and provides a solid foundation for the breeding, asexual reproduction, polyploid breeding, and even genetic engineering breeding research of *Iris tectorum* original species and varieties.

The application belongs to the technical field of plant genetic engineering, and discloses a gene for promoting adventitious root development and application thereof. EuWOX11 The application specifically discloses the application of the gene, a recombinant expression vector containing the gene or an engineered bacterium containing the gene in at least one of promoting adventitious root generation, adventitious root elongation, increasing the number of adventitious roots, increasing the biomass of adventitious roots, and promoting the growth of an aboveground part; the application in at least one of improving the rooting characteristics of woody plants, shortening the rooting cycle of cuttage, increasing the rooting rate of cuttage, increasing the survival rate of cuttage, and increasing the efficiency of asexual reproduction; and a method for obtaining a plant with improved adventitious root development. The transgenic plant obtained by overexpressing the gene has a more developed root system, a more vigorous aboveground part, and stronger adaptability after planting, and can significantly improve the seedling raising efficiency and seedling quality and reduce the production cost, and has important application value and economic value in the molecular breeding and industrial breeding of eucommia ulmoides and populus tomentosa.

The present application relates to a Tibetan white bark pine of the same variety grafting method, and belongs to the technical field of forest tree asexual reproduction. In view of the technical problems of low survival rate of white bark pine grafting, easy microbial infection of grafting interface and slow formation of callus in high altitude environment, the method realizes efficient grafting through the synergistic optimization of pre-treatment of scion and cut processing of stock: 1-year-old branches with a diameter of 4-6mm are selected and treated by temperature and humidity regulation and composite antibacterial liquid immersion, and the cut of the stock is cut at an angle and coated with composite antibacterial liquid four times to form a sterile healing interface; 1-year-old branches are cut, and there are at least two or more bud points on the branches, two full bud points are retained, and the branches are cut between the two full bud points to obtain the scion. The method significantly improves the survival rate of white bark pine grafting and the interface stress resistance in alpine regions, is suitable for the rapid breeding and genetic property reservation of white bark pine excellent germplasm in the ecological restoration engineering of Tibet plateau, and can make more use of and protect the white bark pine shoot resources in Tibet.