59 results about "Asexual reproduction" patented technology

The invention discloses an integrated removal and treatment method for spartina alterniflora in a mudflat wetland. The integrated removal and treatment method sequentially comprises the steps that a construction operation area is divided; mowing the spartina alterniflora for more than three times in the construction operation area, and airing the crushed straws; performing anaerobic landfill on the straws into the first landfill ditch; carrying out more than two times of root system ploughing on the construction operation area which is already cut; a second landfill ditch with the depth not smaller than two meters is dug, and the ploughed spartina alterniflora root system is placed into the second landfill ditch; and covering soil and compacting the second landfill ditch. According to the method, the formation of mature seeds of the spartina alterniflora can be effectively prevented, the sexual propagation path of the spartina alterniflora can be effectively cut off, the surface of the first landfill ditch after landfill is no longer suitable for growth of the spartina alterniflora, mowing and ploughing are carried out in different areas at the same time, the spartina alterniflora is prevented from sprouting again, the treatment efficiency of the wetland spartina alterniflora is further improved, and the economic benefit is increased. Vegetative propagation of spartina alterniflora is avoided, repeated treatment of spartina alterniflora is avoided, and the treatment cost of spartina alterniflora is reduced.

The invention discloses a method for monitoring vegetative propagation growth of roots and stems of bluegrass based on clustering processing. The method comprises the steps of multi-source monitoring data acquisition, multi-source data preprocessing, growth state monitoring clustering model construction, clustering structure optimization and layered clustering growth state monitoring. The invention relates to the technical field of plant propagation data processing, in particular to a method for monitoring vegetative propagation growth of roots and stems of bluegrass based on clustering processing, which innovatively proposes a bi-clustering mechanism based on a main-sub layered structure, and significantly improves the accuracy, stability and agricultural availability of growth state monitoring. A misclassification point recognition mechanism and a misclassification triggering mechanism are designed, and a cluster adjustment factor is introduced, so that the adaptability and the expression ability of a complex plant growth monitoring behavior mode are enhanced; an optimization algorithm and an optimal parameter combination of a growth state monitoring clustering model are improved by adopting a mirror surface reverse mechanism and a sine and cosine function method, and a clustering structure is optimized, so that the accuracy of a growth state monitoring result is improved.

The invention discloses bacillus and application thereof, and belongs to the technical field of microorganisms, the bacillus is preserved in China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20242290, and the preservation date is October 22, 2024. The composition can effectively inhibit vegetative growth and vegetative propagation of pathogenic bacteria such as colletotrichum gloeosporioides and paecilomyces japonicus, and has a good and broad-spectrum control effect on various pathogenic fungi. Bacteriostatic components contained in fermentation filtrate of the bacillus can remarkably inhibit germination of colletotrichum spruce spores and formation of appressorium. And the bacillus has a good prevention and treatment effect on the citrus huanglongbing.

The present invention discloses a plant regeneration method of an excellent clone of Eucalyptus grandis DH32-28: S1: branches of Eucalyptus grandis DH32-28 are collected and disinfected to obtain sterile seedlings; S2: the sterile seedlings are cultured through proliferation to obtain proliferated seedlings, and the proliferated seedlings are inoculated into a GA3 bud elongation medium to obtain elongated seedlings; S3: internode stem segments of the elongated seedlings are taken as explants to induce callus tissue; S4: the callus tissue is inoculated into a regeneration bud induction medium to induce regeneration buds; S5: the induced regeneration buds of a certain length are inoculated into a rooting medium to induce rooting, and the rooted regeneration buds are regenerated plants. The present invention can quickly and effectively regenerate Eucalyptus grandis DH32-28 plants, is easy to operate, and is convenient for mass production of seedlings through asexual reproduction.

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

The application provides a hard branch cutting breeding method of Actinidia kolomikta and Zizyphus jujuba, relates to the technical field of asexual reproduction and seedling raising, and comprises the following steps: (1) collecting 1-2 year old branches and pruning branch tips; (2) cutting into 10cm cuttings with more than two bud points, with the upper end of the cutting being inclined and the lower end being horizontal, and soaking the cutting in carbendazim for sterilization treatment; (3) selecting grass charcoal mixed with perlite as a cutting substrate, disinfecting the substrate after being loaded into a plug tray; (4) preparing a rooting agent, treating the cutting of step (2), inserting the cutting into the substrate of the plug tray, fixing the cutting, pouring carbendazim through the substrate, and covering the cutting with a transparent cover; (5) placing the cutting under the condition of 70% shading, increasing 20% illumination after new leaves are unfolded, controlling the temperature to be 10-30 DEG C, timely watering the cutting after cutting, combining with carbendazim sterilization, gradually opening the transparent cover after 40d of cutting, watering according to the situation, and spraying foliar fertilizer every 10d; and (6) transplanting and planting the cutting after 60d of cutting. The application has the beneficial effects of simplifying the hard branch cutting steps of Actinidia kolomikta and Zizyphus jujuba and shortening the cutting breeding period.

The invention relates to the technical field of traditional Chinese medicinal material planting. The invention provides a container confinement cultivation method for relieving asexual propagation continuous cropping obstacles of gastrodia elata, which comprises the following steps: (1) filling and paving planting soil in a container, placing wood sections and armillaria mellea, then earthing, carrying out heat preservation treatment, and culturing to obtain a fungus bed; and (2) exposing the fungus material, placing gastrodia elata seeds around the fungus material, covering soil, carrying out heat preservation treatment, and carrying out conventional planting management. By means of the cultivation method, centralized and standardized cultivation of the gastrodia elata can be promoted, the problem that suitable cultivation sites are gradually reduced in the gastrodia elata cultivation process is solved, the problem of continuous cropping obstacles of the gastrodia elata is effectively solved, and sustainable development of the gastrodia elata is promoted.

The invention provides a red and blue lycoris radiata tissue culture method, and relates to the technical field of plant propagation, the method sequentially comprises the steps of explant collection and pretreatment, explant disinfection, induction culture, multiplication culture, strong seedling culture and rooting culture, aiming at different requirements of each culture stage, a low-nitrogen / high-nitrogen adaptive culture medium system is customized, and the culture medium system is used for culturing the red and blue lycoris radiata. According to the method, the hormone proportion is accurately regulated and controlled, functional components such as acidolysis casein, coconut juice and activated carbon are added, meanwhile, culture environment parameters are optimized, an asexual propagation mode is adopted, efficient propagation of the lycoris radiata buds can be rapidly achieved, the problem of activity decline after long-term propagation is solved, the risks of pollution, browning and vitrification are effectively reduced, and the survival rate of the lycoris radiata buds is increased. The robustness and transplanting survival rate of tissue culture seedlings are improved, and meanwhile, stable inheritance of excellent characters of varieties is guaranteed.

This invention discloses the agronomic traits, InDel-labeled fingerprint, and construction method of the *Dictyophora indicum* strain YZS021, belonging to the field of *Dictyophora indicum* detection technology. The fingerprint is obtained by PCR amplification and electrophoresis detection using 10 pairs of InDel-labeled primers developed based on the whole genome of *Dictyophora indicum*. The InDel marker fingerprint spectrum detection of the *Dictyophora indicum* strain YZS021 of this invention is fast, accurate, and reproducible, and can specifically identify the strain, enabling precise identification of the fungal species and protection of intellectual property rights. In addition, this invention also discloses the agronomic traits of the *Dictyophora indicum* strain YZS021. The *Dictyophora indicum* strain 'YZS021' has a long and thick stipe, a high edible ratio, and good commercial characteristics. It can stably produce fruiting under both forest and facility cultivation modes, adapting to different cultivation scenarios and significantly improving planting efficiency. The above agronomic traits have consistency and stability, and can stably cultivate and obtain asexual reproduction lines with the above traits in both forest and facility fruiting rooms.

This invention relates to a rooting agent and seedling cultivation method for promoting rooting of *Cyclocarya paliurus* cuttings, belonging to the field of plant asexual reproduction technology. It solves one of the problems in existing *Cyclocarya paliurus* seedling cultivation: low rooting rate, long rooting time, few and poor-quality adventitious roots, and low transplant survival rate. This invention discloses a rooting agent for promoting rooting of *Cyclocarya paliurus* cuttings, which consists of two components, A and B. Component A consists of potassium indolebutyrate, soluble starch, chlorogenic acid, salicylic acid, and talc; component B consists of indolebutyric acid, sucrose, chlorogenic acid, vitamin C, and talc. Using the above-mentioned rooting agent and the corresponding seedling cultivation method, rapid rooting of *Cyclocarya paliurus* cuttings is achieved, with a rooting rate as high as 93.06%, and a large number and good quality of adventitious roots, resulting in a transplant survival rate of 90.37%.

The invention provides an idesia grafting seedling raising method, relates to the technical field of grafting seedling raising, and adopts the technical scheme that the method comprises the steps of scion type determination, grafting method determination, grafting time determination and the like. According to the statistical data of the survival rate, the determination of appearance growth indexes of the grafted seedlings, and the fruit yield and quality, the optimal grafting mode is determined to be grafting in an axillary bud cut-grafting mode at the beginning of spring. The grafting combination most suitable for idesia polycarpa branch grafting is researched from different grafting periods and different grafting methods, a set of grafting method suitable for idesia polycarpa branches is established, and a set of feasible grafting method capable of achieving industrialized seedling raising is provided for asexual propagation of idesia polycarpa. And the method has important practical significance on the popularization of improved varieties of idesia polycarpa, the preservation of germplasm resources and the improvement of industrial quality and efficiency.

The invention discloses a cutting propagation method for sinojackia xylocarpa, and belongs to the technical field of plant asexual propagation. The cutting propagation method of the sinojackia xylocarpa comprises the following steps: immersing cutting slips of the sinojackia xylocarpa into an ethylicin solution, and then inserting the cutting slips into a cutting medium for cultivation. By means of the cutting propagation method, the cutting rooting rate of the twigs of the sinojackia xylocarpa can be remarkably increased by controlling the cutting time, the cutting environment, the cutting treatment, the cutting medium and other factors, the highest cutting rooting rate after two months can reach 90%, and the highest cutting rooting rate after three months can reach 80% or above. The cutting propagation method provided by the invention is simple and rapid, low in cost and easy to implement on a large scale.

The invention belongs to the technical field of fruit cultivation, and particularly relates to an avocado rootstock asexual propagation method which comprises the following specific steps: material preparation, grafting and yellowing treatment, cup sleeving of a micro container and rooting promotion, rooting inspection, scion grafting, cutting and independent seedling hardening, and outplanting and transplanting. Compared with vegetative propagation, seedling propagation and tissue culture, the method has the advantages of high efficiency, multi-bud synchronization, high sapling consistency, root system and crown balance, full play of genetic potential, low transportation cost, high quarantine safety, good root system quality, high field planting survival rate and no rejuvenation period, and is suitable for large-scale, commercial, long-distance transportation and facility cultivation.

This invention discloses a species of Armillaria mellea ZM51, whose classification name is [not specified]. French Armillaria This strain was deposited on May 8, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.42001, located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. *Armillaria galbana* ZM51 exhibits unique characteristics such as rapid mycelial germination, strong resistance to contamination, and early fruiting. Through multiple generations of asexual reproduction, its traits remain stable and consistent. Compared to the existing *Armillaria olfleurone* 'Hazelnut-4', its mycelial germination time is shortened by 3-6 days, and fruiting occurs approximately 20-25 days earlier. In Liaoning province, through semi-wild cultivation, it achieves autumn fruiting after spring sowing, with clustered fruiting bodies, significantly larger mushrooms than 'Hazelnut-4', thicker stipes, and larger caps, demonstrating significantly enhanced resistance to contamination.

The invention relates to the technical field of short-petal rose cultivation, discloses a short-petal rose cultivation method and aims to stably cultivate short-petal roses. The method comprises the following steps: selecting a land parcel in which rosaceae and Chinese rose plants are not planted in recent three years, deeply ploughing, applying a base fertilizer, disinfecting by solar energy, and ventilating for later use; a greenhouse is built in a standby land parcel, parallel ridge bodies with the ridge spacing of 0.6-0.8 m and the ridge height of 25-35 cm are arranged in the greenhouse, a drip irrigation system is arranged, and the ridge surfaces and furrows are covered with grass-proof cloth; the method comprises the following steps: taking more than 6 generations of asexually propagated 1-year-old short-petal rose stock plant stems, disinfecting and promoting roots to obtain cutting seedlings, planting the cutting seedlings at 15-25 DEG C according to the row spacing of 0.4-0.6 m, and recovering the seedlings; performing timed drip irrigation, periodic fertilization, branch pruning and pest control on the short-petal rose young plants after seedling recovery to obtain short-petal rose adult plants. The method is standard in operation, capable of stably cultivating the short-petal roses and suitable for large-scale cultivation.

The invention relates to the technical field of plant breeding, and discloses an oriental cherry rhododendron cutting seedling raising method and application thereof.The method comprises the steps of S1, facility and substrate preparation, specifically, seedling raising is conducted in a greenhouse, and Kanuma soil is selected as a cutting substrate; s2, cutting preparation: from mid-to-late May to early-to-mid September, selecting annual branches with semi-lignified upper parts and no plant diseases and insect pests of a rhododendron simsii plant as cuttings, cutting the cuttings into cuttings with the length of 10-15cm, reserving leaves on the cuttings, performing cutting seedling raising of the rhododendron simsii in a greenhouse, preparing an advanced monitoring system and a control system, and performing cutting seedling raising of the rhododendron simsii in the greenhouse. Air temperature and humidity, soil temperature and humidity and illumination intensity can be accurately monitored in real time, the cuttage method provides an effective way for vegetative propagation, excellent characters of stock plants can be reserved, meanwhile, direct digging of wild oriental cherry rhododendron in the cold highland area can be reduced through manual cuttage propagation, local fragile ecological balance can be maintained, and the survival rate of the wild oriental cherry rhododendron in the cold highland area is increased. And a virtuous circle of protection and utilization is realized.

The invention discloses an application of rice OsTIP2; the invention discloses application of the gene 1, 2 in improving the resistance of rice to acetochlor and a rice breeding material thereof. The method comprises the following steps: knocking out OsTIP2; according to the OsTIP2 gene, the resistance of rice to acetochlor can be improved; the amino acid sequence of the gene 1, 2 is as shown in SEQ ID NO: 2; and by matching with acetochlor for use, simplified planting of the rice can be assisted, and important economic value is achieved. The herbicide resistance characteristic of rice edited by the gene can be stably inherited through sexual or asexual reproduction, and the gene can be used for constructing resistant rice germplasm resources.

The invention discloses a high-quality tomato inbred line breeding method based on wolfberry fruit eccDNAs transfer, and belongs to the field of plant biological breeding and traditional breeding, the method comprises the following steps: grafting by using tomato as a scion and wolfberry fruit as a rootstock, carrying out soil burying overwintering cultivation to induce the scion above a graft union to form a regenerated adventitious bud, and carrying out high-quality tomato inbred line breeding. Taking regenerated adventitious buds for asexual propagation, then performing continuous selfing, screening selfing lines with superior characters based on fruit morphology, physiological characteristics and quality indexes, and performing wolfberry eccDNAs verification on second-generation selfing plants. The high-quality flavor tomato inbred line with genetic stability is obtained by optimizing the breeding method, a foundation is created for research and development of new varieties, and the method has good popularization and application prospects.

The invention relates to the technical field of vegetative propagation of plants, and particularly discloses a method for promoting growth of a peony high-altitude layering propagation root system by using a humic acid-rich fertilizer solution, and the method adopts a humic acid-containing water-soluble fertilizer to promote rooting. In order to solve the problems of low propagation coefficient, complicated operation, high labor cost and the like of a traditional peony propagation method, especially a high-altitude layering propagation method, and the risk of hormone rooting agent residue, a humic acid-containing water-soluble fertilizer is innovatively adopted as a root promoting agent to smear girdling cuts below mixed buds to promote rooting. The method solves the technical bottlenecks of few induced root systems and slow growth in high-altitude layering propagation of the peony, is suitable for commercial seedling culture of the peony, protection of endangered wild species, propagation of hybrid superior plants and the like, and provides technical support for efficient propagation of the peony.

The invention belongs to the technical field of plant asexual propagation, and particularly relates to a bougainvillea speetabilis cutting propagation method. According to the method, current-year-old semi-lignified branches on the periphery of a bougainvillea speetabilis crown are cut as cutting slips, after the bases of the cutting slips are soaked with an indoleacetic acid solution with the mass concentration of 200 mg / L, the cutting slips are inserted into a yellow subsoil matrix in a cuttage mode, and the cutting slips are compacted, watered thoroughly and maintained every day. Cuttage seedlings obtained through rooting culture for 45 days can achieve core indexes that the number of roots is larger than or equal to 14, the longest root length is larger than or equal to 6.58 cm, the root water content is larger than or equal to 71.61%, the number of sprouts is larger than or equal to 3, and carotenoid is larger than or equal to 2.66 mg / g. According to the method, the problem of shortage of lignified cutting slips in bougainvillea speetabilis cutting propagation is solved, the problem of character variation is solved, the seedling raising cost is remarkably reduced through a standardized process, and efficient and reliable technical support is provided for large-scale production of bougainvillea speetabilis.

The present invention discloses a method for monitoring the asexual reproduction and growth of rhizomes of bluegrass based on clustering processing, and the method includes multi-source monitoring data acquisition, multi-source data preprocessing, construction of a growth status monitoring clustering model, clustering structure optimization and hierarchical clustering growth status monitoring. The present invention relates to the technical field of plant reproduction data processing, and specifically refers to a method for monitoring the asexual reproduction and growth of rhizomes of bluegrass based on clustering processing. This solution innovatively proposes a dual clustering mechanism based on a master-sub hierarchical structure, which significantly improves the accuracy, stability and agricultural usability of growth status monitoring; designs a misclassification point recognition mechanism, a misclassification trigger mechanism and introduces a cluster adjustment factor to enhance the adaptability and expression ability of complex plant growth monitoring behavior patterns; adopts a mirror inversion mechanism and a sine-cosine function method to improve the optimization algorithm, the optimal parameter combination of the growth status monitoring clustering model, optimizes the clustering structure, and improves the accuracy of the growth status monitoring results.

The application provides a breeding method and application of a young plant of a cow ear. The application adopts a leaf of the cow ear as an explant, optimizes a breeding technical system through induction of germination of double-face clustered buds of the leaf, and obtains a large amount of high-quality young plants. The method can overcome physiological time limitation of plant material taking, and will not affect species quantity and reproduction. Through induction of the double-face clustered buds of the leaf, a new way of asexual reproduction of the plant leaf is explored, utilization efficiency of the explant of the plant tissue culture is increased, and a simple, rapid and effective breeding system of the cow ear is established.

The invention relates to a group of SNP (Single Nucleotide Polymorphism) molecular markers associated with surface area characters of poplar roots and application, and belongs to the technical field of genetic breeding of poplar. The method comprises the following steps: identifying key sites influencing the root surface area through whole genome association analysis, and respectively positioning the key sites at the 10391007bp (SAsite1) and the 10391759bp (SAsite2) on the No.6 chromosome of a populus sibirica genome; the two sites are completely linked, and the root surface area of the heterozygous haplotype (GA / CG) is obviously larger than that of the homozygous haplotype (GG / CC). The molecular marker provided by the invention can be used for early molecular auxiliary selection of the surface area of the root of the poplar, is beneficial to accurately and efficiently screening a poplar clone with a relatively large root surface area, and provides reliable information for improving the survival rate of vegetative propagation and breeding an excellent strain.

To provide a procedure for inducing apomixis (also called agamospermy) in crops, and a method for producing an apomictic plant and an apomictic seed, and also provide a gene and a protein involved in the process of apomixis, particularly in parthenogenesis.SOLUTION: The invention provides nucleotide sequences and amino acid sequences of the parthenogenesis gene of Taraxacum as well as (functional) homologues, fragments and variants thereof, which provide parthenogenesis as a part of apomixis. Also parthenogenetic plants and methods for making these are provided, as are molecular markers and methods of using these.SELECTED DRAWING: None

The invention belongs to the field of blue fescue development, and provides a method for promoting blue fescue fetal inflorescence development through a plant hormone formula, and the method comprises the following steps: selecting a blue fescue parent plant before flower bud differentiation, preparing a plant hormone solution which contains abscisic acid and gibberellins, the concentration ratio of the abscisic acid to the gibberellins is 3: 1, and the plant hormone formula is used for promoting the blue fescue fetal inflorescence development. The maternal plants of the blue fescue are subjected to hormone irrigation treatment, specifically, the maternal plants of the blue fescue are irrigated once every three days, an abscisic acid solution is irrigated firstly, then a gibberellin solution is irrigated, and the irrigation period lasts for 2 months; according to the method disclosed by the invention, the maternal plant of the fescue is treated by the plant hormones with a specific ratio, so that the generation quantity of fetal buds on the vegetative body fetal inflorescence is remarkably increased, the asexual propagation coefficient of the fescue is rapidly expanded in a relatively short time, and meanwhile, the excellent horticultural characters of the female parent can be kept by propagated offspring; therefore, the market supply capability and the economic benefit of the festuca arundinacea seedlings are effectively improved.

The application belongs to the technical field of plant tissue culture and particularly relates to a tissue culture propagation method of Cupressus gigantea. In order to solve the technical problems of difficult asexual reproduction, low seed germination and rooting rates of Cupressus gigantea, the application provides the tissue culture propagation method of Cupressus gigantea, which comprises the following steps: taking Cupressus gigantea seeds or branches as explants, sterilizing the explants, and then sequentially performing initiation culture and rooting culture; when the Cupressus gigantea seeds are taken as the explants, the initiation culture further comprises proliferation culture. The application obtains the optimal tissue culture condition of Cupressus gigantea by screening the sterilization mode, the initiation culture medium, the proliferation culture medium and the rooting culture medium, and the survival rate of transplanting is more than 90% after seedling transplanting and local transplanting. The application provides technical support for the mass propagation of Cupressus gigantea and has important significance for the field return and population recovery of Cupressus gigantea.

The present application relates to the technical field of plant asexual reproduction, and particularly discloses a cutting propagation method for Lindleya benzoe. The method comprises the following steps: pretreating the base of cuttings with an activating solution containing oligosaccharide signal molecules and active components of root exudates of Lindleya benzoe, then cutting the cuttings in a special induction matrix containing slow-release plant growth regulators, and performing programmed induction of dark culture and light culture; during the light culture stage, dilute solution of the activating solution is applied to the base of the cuttings as an auxiliary inducer. The present application integrates endogenous signal activation, slow-release continuous supply and phased synergistic induction, systematically simulates and strengthens the chemical biological microenvironment of natural rooting, effectively activates the endogenous root potential of the cuttings, and solves the technical problems of low rooting rate, poor root quality and weak transplanting adaptability in conventional methods, thereby realizing efficient and high-quality propagation of Lindleya benzoe cutting seedlings.

The invention belongs to the technical field of plant asexual propagation, and relates to a method for rapid propagation by utilizing triarrhena lutarioriparia stem tip tissue culture. The method comprises the following steps: (S1) cultivating triarrhena lutarioriparia stem segments with axillary buds into seedlings through cuttage, taking stem tips, and disinfecting the stem tips with a botanical disinfectant to obtain triarrhena lutarioriparia stem tip explants; (S2) inoculating the triarrhena lutarioriparia stem tip explant into a callus induction culture medium containing 10-20mg / L of tea polyphenol, and inducing to form a callus; (S3) cutting the callus, inoculating the cut callus to a differential medium containing 0.5-0.8 g / L of proline and 3-5g / L of trehalose, and differentiating to obtain triarrhena lutarioriparia differentiated seedlings; (S4) transferring the triarrhena lutarioriparia differentiated seedlings into a rooting culture medium to induce rooting, so as to obtain triarrhena lutarioriparia stem tip tissue culture seedlings. According to the method, the stem tips of the cutting seedlings serve as explants, a specific disinfection process and an optimized culture medium are comprehensively adopted, a stable and efficient triarrhena lutarioriparia rapid propagation system is constructed, the problems that traditional explants are limited by seasons, the seedling rate is low and the like are solved, and technical support is provided for industrial propagation of triarrhena lutarioriparia.

The application discloses a jellyfish polyploid induction method and application thereof, and belongs to the technical field of marine ornamental animal breeding. The jellyfish polyploid induction method takes the polyp stage of jellyfish as an induction object, and polyploid jellyfish is obtained by induction treatment through combination of colchicine and dimethyl sulfoxide. The method can obtain multiple polyploid new germplasms through one treatment under the use of a small amount of inducers. The obtained polyploid polyps can not only rapidly proliferate new individuals with polyploid characteristics through asexual reproduction, but also obtain jellyfish bodies with polyploid characteristics, such as fast growth speed, long life cycle, beautiful shape and large individual. Meanwhile, the method has the characteristics of simple operation and low cost. Therefore, the induction method has great application value and broad application prospect.

This invention belongs to the field of crop genetics and breeding technology, and particularly relates to a method for breeding large-fruited thornless prickly pears using thornless prickly pear germplasm resources. The method includes the following steps: screening key parents from the seedling progeny population of thornless prickly pear germplasm, from which individuals exhibiting the thornless trait can be isolated; germinating the seeds of the key parents' progeny until they show white sprouts, and then chemically mutagenesis using colchicine solution to obtain a first-generation mutant population; selecting mutant plants from the first-generation mutant population with completely thornless fruit and a single fruit weight of not less than 20 grams; and performing asexual reproduction on the mutant plants, verifying through multiple generations of cultivation to obtain varieties with stable thornless and large-fruit traits. This invention effectively solves the technical problem of small fruit size and difficulty in genetically fixing the thornless trait in breeding from thornless prickly pear germplasm resources, creating a new prickly pear germplasm with large fruit size, thornlessness, and stable traits.