251 results about "Asexual reproduction" patented technology

The invention discloses a method for breeding tetraplont locust own-rooted plantlets by means of hardwood cutting; in the invention, a root nursery is established by planting the own-rooted plantlets,well-bred roots are collected as breeding materials, and a plant growth regulator is added in a matching manner to realize asexual reproduction of the tetraplont locust in large scale. The method obtains the tetraplont locust own-rooted plantlets by tissue culture or hardwood cutting; the own-rooted plantlets are planted to form the root nursery; and the fertilizer and water management of the root nursery is strengthened to accelerate the growth of the plantlets. After two years, multitudinous root segments can be provided every year; the robust root systems of which root diameters reach 0.3cm are screened in the root system collection in spring, then the root systems are pruned into root segments with a length of 5-6cm and planted in a seeding nursery; and most plantlets grew in currentyear can be as high as 1.0m and the ground diameter can reach 0.8cm, which can be used for forestation. The method for breeding tetraplont locust plantlets has the advantages of simple operation, reasonable design and remarkable effect and greatly improves the breeding efficiency of well-bred tetraplont locust plantlets.

The invention relates to the technical field of traditional Chinese herbal medicine cultivation, in particular to a herbahouttuyniae cultivation method, which comprises the following steps of: 1) determining a planting time: planting seedlings at a period from the middle ten days and last ten days of September to the first ten days of November every year; 2) determining a planting mode: selecting high-stalk crops for interplanting; 3) preparing the seedlings: conducting asexual reproduction by using subterraneous stems of herbahouttuyniae as reproduction materials; 4) planting the seedlings: directly placing the seedlings on compartment surfaces and covering the compartment surfaces with rice straw or maize stalks after the seedlings are planted; 5) conducting field management: removing weed at the growth period of the herbahouttuyniae, removing ill herbahouttuyniae plants, weak herbahouttuyniae plants and non-herbahouttuyniae crops during weeding, and making sure that the herbahouttuyniae is not damaged; and during top dressing, using farm manure as main fertilizers and chemical fertilizers as auxiliary fertilizers, paying attention to reasonable drainage and irrigation and keeping soil wet at the entire growth period of the herbahouttuyniae; and 6) harvesting at a proper time and the like. By adopting the herbahouttuyniae cultivation method, the yield of the herbahouttuyniae is high, the damage by diseases and insects is reduced, the herbahouttuyniae diseases such as southern blight and zonate spot can be effectively prevented and controlled, and the yield of the herbahouttuyniae and the mass content of medicinal components in the herbahouttuyniae are improved to the utmost extent.

The invention relates to a forest tree seedling breeding technology and in particular relates to a method for suppressing browning of a carya cathayensis sarg explant. The method is characterized by selecting a carya cathayensis sarg stem with axillary buds as an explant in spring, disinfecting the explant, then inoculating the explant into a culture medium to which catechins and anti-browning agents are added, and firstly culturing a tissue culture vessel in the low temperature dark environment and then transferring the tissue culture vessel to the routine culture environment at the initial stage of inoculation. The method can conduce to obvious reduction of the browning rate of the carya cathayensis sarg explant and improvement of inductivity of the carya cathayensis sarg explant, is simple and convenient to operate, has a better browning suppression effect, and provides a technical support for tissue culture and asexual reproduction of carya cathayensis sarg.

The present invention is directed to the seed-to-seed perpetuation of hybrid vigor and other traits through apomixis (asexual seed formation) in flowering plants (angiosperms). More particularly, to predictable methods for producing, from sexual or facultatively-apomictic plants, progeny plants that express an increased percentage of apomictic seed set or one or more elements of apomixis. This invention uses: plant cyto-embryology procedures to identify and select a plant or group of plants that possess appropriate genetic variability for initiation times and durations of megasporogenesis (female meiosis), embryo sac formation, egg and central cell formation and maturation, fertilization, embryony and endosperm formation; plant breeding procedures to produce numerous and divergent genetically-recombined early to late generation progeny such that embryo sac formation preempts megasporogenesis and embryony preempts fertilization; and plant cyto-embryology or progeny test procedures to select segregant plants that express an increased frequency of one or more elements of apomixis.

The invention relates to a cultivation method of the triploid salvia, the steps of the invention are that: not completely ripe good salvia seeds are collected to put into the 1/2MS culture medium with colchicine for mutagenesis cultivation; then the seeds are transferred into a normal 1/2MS culture medium for cultivation, the root tip of the seedling is fetched to perform the ploidy identification of the chromosome after the preceding process of the chromosome, and the seedling with a chromosome quantity of 2n=4x=32 is the tetraploid salvia; the tetraploid salvia is taken as a female parent and the diploid salvia is taken as a male parent to perform the crossbreed and the obtaining seed is a triploid salvia plant. The nutrient organs such as the root, stem and leaf and so on of the triploid salvia plant obviously become bigger and present remarkable crossbreed advantages, and the root medicinal material output of the triploid salvia is increased by over 30 percent. Moreover, the triploid salvia has flowers, but no seeds, thus decreasing the nutrition consumption and the crossbreed advantages of the triploid salvia can be kept over 10 years through the asexual reproduction, which enhances the disease resistance and stress resistance with strong applicability, thus being applicable to planting in every part of the country. Besides, the crossbreed advantages produced by the novel variety triploid production are able to improve the output of the salvia medicinal material planting in a large scale.

The invention discloses a high-yield Magnolia officinalis propagation method, which relates to the technical field of plant propagation. The propagation process includes seed selection, seed treatment, selection and retention of female parent saplings, establishment of collection gardens, tree planting screening, and asexual propagation; it has a propagation process The invention has the characteristics of being simple, capable of breeding magnolia saplings with good resistance, increased weight of effective parts, and high active ingredient increment, and can be used for breeding high-yield magnolia saplings and other plants.

The invention discloses technology for breeding heartleaf houttuynia herb seedlings, and relates to the fields of biotechnology and crop cultivation. The technology comprises seed collection, seed storage, seed sterilization, seed low-temperature treatment, seed germination, seedling cultivation and other processes, wherein the mature and dry heartleaf houttuynia herb seeds are sterilized by 70 percent ethanol and 0.1 percent mercuric chloride, are washed by sterile water for 4 to 5 times, and are treated at low temperature of between 1 and 4 DEG C for 7 days; and the seeds are sowed on an MS minimal medium and are transplanted to a seed bed for culturing seedling 14 days after the seeds are germinated in an illumination incubator. The technology treats the heartleaf houttuynia herb seeds at low temperature, changes the physiological status of the seeds, and promotes the seeds to germinate and grow to form seedlings. The seed breeding method can also avoid the problem of plant diseases and insect pests due to the fact that insect sources of a stock plant are easily brought to the seedlings by rhizome asexual reproduction, and can reduce planting cost simultaneously.

The invention discloses a method for regenerating a plant by use of the callus of camellia japonica, belonging to the field of biotechnology. The method is characterized by comprising the following steps of: collecting the fruits of camellia japonica in early September, and disinfecting and cleaning; removing the inner and outer seed coats of seeds, and inoculating to a callus induction medium; transferring the material to a callus transition medium in the 25th -28th day; after the callus turns from light yellow into yellow with a little red, transferring the material into a callus differentiation adventitious bud medium; after the material is formed and bud sticks of 3-4cm grow, soaking the base part in IBA, and transferring to a paper bridge of an MV liquid medium to induce rooting; starting in the 20-22 days to grow transparent radicles with a little red; and then culturing for a certain period of time so that the radicels extend and are gradually lignified. The method for regenerating a plant by use of callus of camellia japonica, disclosed by the invention, provides a process with a relatively short period and a relatively high reproduction coefficient to large-scale asexual reproduction of camellia japonica, and lays a foundation for the molecular breeding of camellia japonica.

The invention discloses a culture medium for promoting an adventive root of a woody plant to root and grow and an application of the culture medium. The culture medium comprises the following components: inorganic salt A, inorganic salt B, an organic compound, 50-150mg/L water-soluble carbon nano tubes, 0.5-1.5mg/L indolebutyric acid, 100mg/L 2-(N-morpholine) ethanesulfonic acid, 30000mg/L cane sugar and 5000mg/L agar. The culture medium provided by the invention is more capable of promoting the growth of the adventive root. The largest difficulty of asexual reproduction of the woody plant lies in the rooting and the growth of the adventive root, the culture medium has the same promotion effect for the rooting of the adventive root of brassaia actinophylla and the rooting and the growth of plants such as honeysuckle, blueberry, small kiriko, swida wilsoniana, zanthoxylum dissitum and the like.

The invention discloses a method for breeding an improved variety of scrophularia ningpoensis Hemsl., and solves problems that due to long time asexual reproduction of scrophularia ningpoensis Hemsl. in the prior art, virus accumulation is serious, and the yield and the quality are reduced. The method comprises the following steps: 1), screening plants, wherein the pest-free plants are selected; 2) pre-treatment and disinfection, wherein scale-peeled buds are soaked with the 20-time sodium hypochlorite solution for 8-10 min; 3) stem tip peeling, wherein each stem tip with 1-2 leaf primordium are cut; 4) cultivation, wherein the stem tips are placed in a culture medium for cultivation; 5) exercised seedling transplantation, wherein the seedlings are cultivated in the sun, then the seedlings are transplanted to a matrix to be cultivated for 15 days, and finally the seedlings are transplanted to a field. Compared with the conventional asexual reproduction method for scrophularia ningpoensis Hemsl., the yield and the quality of the scrophularia ningpoensis Hemsl. seedlings cultivated by the method are greatly improved.

The invention relates to a grafting method capable of promoting asexual reproduction of populus euphratica. The method comprises the following steps: (1) irrigation and pest control before grafting; (2) selection of a rootstock: selecting annual Populus bolleana as the rootstock, wherein a tree trunk of the Populus bolleana is straight from top to bottom, and the Populus bolleana is vigorous in growth and full in scion; (3) selection of populus euphratica scion: selecting a bud as the populus euphratica scion, wherein the middle portion of a seeding branch is full, the seeding branch has 4-6 buds and lengths of the buds is 8-12 millimeters; (4) cutting a cross-shaped incision on a rootstock Populus bolleana trunk at a height of 1-1.2 meters with a grafting knife, and levering the incision; (5) clipping the lower end of a populus euphratica scion lower bud into a rhombus and obtaining a clipped populus euphratica scion; (6) inserting the clipped populus euphratica scion into the incision position around the Tomb-sweeping Day and binding tightly, and then the grafting is finished; (7) irrigating and fertilizing timely after the grafting is finished; and wiping off all the buds ready to sprout when the populus euphratica starts to sprout, and spraying pesticides when 3-4 true leaves grow on the populus euphratica seedling. The grafting method capable of promoting the asexual reproduction of the populus euphratica can effectively stop a populus euphratica population from being weak in regeneration and aging.

The invention discloses a non-tube cutting seedling method of tissue subcultured buds of zenia. The two steps of non-tube rooting of tissue subcultured buds and cutting seedling are combined into one. The tissue subcultured buds of zenia are bred by a conventional method, and then the tissue subcultured buds of zenia are selected as cuttings and are treated by a root promoter and cut into a red subsoil matrix, and seedlings are cultured to obtain forestation wood seedlings for planting. According to the method, the tissue subcultured buds of zenia are directly used as the cuttings for non-tube cutting, the breeding speed is fast, and seasonal and geographical restrictions do not exist; and meanwhile, the two steps of non-tube rooting and cutting seedling are combined into one, the method is simple, the operation is easy, rooting starts after cutting culture for 15 to 20 days, the seedling survival rate is high, and the rooting survival rate is 85% to 95%. The method overcomes the defects of the traditional zenia asexual reproduction method, the seedling cycle is shortened, the seedling survival rate is improved, the seedling cost is reduced, and the method is favorable for accelerating the development of asexual seedling of zenia.

The invention relates to a method for obtaining hydranths of mastigias papua, and belongs to the field of artificial feeding and propagating of ornamental marine animals. The method comprises the steps of: culturing parents in a manner of completely being away from light, wherein the culturing water temperature is 25-28 DEG C, enabling the cultured parents to be fertilized, and when fertilized eggs grow into planula larvas floating and swimming on a water surface, taking out the parents; shifting the planula larvas into fresh seawater with the same temperature, at the same time, putting attaching pieces in the fresh seawater, wherein the putting density is that 400-600 planula larvas and two attaching pieces are put in each one liter of a container, and culturing the planula larvas at a constant temperature; when the planula larvas grow into the hydranths with 4 feelers in an attaching manner, beginning to feed the hydranths with artemia nauplii every day, after feeding the hydranths with the artemia nauplii for 2.5-3.5 hours, completely changing water, when the hydranths grow to have 16 feelers, controlling the culturing temperature to be 20-27 DEG C, and extending the number of the hydranths through asexual reproduction. According to the method disclosed by the invention, the hydranths of the mastigias papua can be cultured in batches, and the requirements of showing, enjoying and researching the hydranths of the mastigias papua can be met. The breeding blank of the mastigias papua is filled.

The invention relates to the technical field of asexual reproduction of seaweed artificial seedlings, and particularly relate to a seedling cultivation method for Sargassum vachellianum greville by using tissue culture. The method comprises acquiring fully grown wild Sargassum vachellianum greville with immature receptacles, holding culture, accelerating maturation, obtaining oosperms for static cultivation, and obtaining seedlings; segmenting the blades of seedlings of low age in days and the holdfasts of seedlings of low age in days for tissue culture, and obtaining regenerated seedlings; separating the regenerated seedlings from tissue blocks, culturing in vitro, obtaining complete seedlings, and holding culture; segmenting the blades of complete seedlings in holding culture again for tissue culture, and obtaining regenerated seedlings again; and finally separating the regenerated seedlings from tissue blocks, culturing in vitro, inducing the bases of the regenerated seedlings to regenerate holdfasts to form complete seedlings, and obtaining complete seedlings for another tissue culture. The blades and the holdfasts of the regenerated seedlings obtained by using the method of the invention can be used as regeneration sources for continuous use for many times, and are large in amount and easy for storage; the reproduction time is short, the acquisition of seedlings are not limited by objective conditions such as seasons, ecological conditions and the release amount of generative cells.

The invention provides a hibernating conservation asexual reproduction method for jellyfish hydranth. The method is characterized in that when normal cultivation of young jellyfish is finished in early summer every year, an enough quantity of jellyfish intestinal-shape young fingerlings are purchased and subjected to conservation, illumination, feeding, trace-amount inflation, water changing, asexual reproduction and development under the condition of low temperature. The method improves the efficiency and benefit of cultivating jellyfish fingerlings by reducing or avoiding a sexual reproduction process of fingerling cultivation, has a simple structure, reduces production cost, and is suitable for extensive popularization.

The use of ortho-phenylphenol and/or derivatives thereof to inhibit the asexual reproduction of fungi, and to filter media, construction materials, construction adjuvants, textiles, furs, paper, hide, or leather. Also, washing agents, cleaning agents, rinsing agents, hand washing agents, hand dishwashing agents, automatic dishwashing agents, and agents for finishing construction materials, construction adjuvants, textiles, furs, paper, hides, or leather, that contain ortho-phenylphenol and/or derivatives thereof,

The invention discloses an agrobacterium-mediated spirodela polyrhiza stable transformation system establishment method, which comprises the following steps: A, inducing calli of spirodela polyrhiza; B, connecting a target gene with a T vector; C, connecting the target gene with a plant expression vector; D, transforming the plant expression vector in agrobacterium tumefaciens LBA4404; E, preparing an infective bacteria solution; F, co-culturing the calli and the agrobacterium tumefaciens; G, performing regeneration and selective culturing: determining that the target gene is successfully integrated into a genome of the spirodela polyrhiza if a GUS (glucuronidase) staining result of thalli is blue, and determining that a part which is stained to be blue by thallus tissues is a part expressed by the target gene. The target gene is inserted into the plant expression vector and then transferred into the agrobacterium tumefaciens LBA4404 for callus infection, and the concentration of antibiotics is finally controlled to screen a positive plant to obtain transgenetic spirodela polyrhiza, and an established transformation system can still ensure continuous and stable inheritance after asexual reproduction of the spirodela polyrhiza, and has bio-safety and development and application value.

The invention provides an imitation wild gastrodia elata cultivation method, and belongs to the field of Chinese herbal medicine planting. The method comprises the following steps that 1, a bacteria bed is arranged; 2, a fungus material is laid flat, and amillaria mellea cultispecies are inoculated; 3, endophytic fungus cultispecies and gastrodia elata capsules are mixed, and seed dressing endophytic fungi are obtained; 4, a layer of seed dressing endophytic fungi are laid flat on the fungus material, a layer of leaves are scattered, and earthing is conducted; 5, the bacteria bed is dug out, white hemp is fixedly planted at the two ends of the fungus material, a bacteria introduction material is placed, and earthing is conducted; 6, harvesting is conducted. Accordingly, when gastrodia elata is planted, sexual propagation and asexual reproduction can be conducted synchronously, the land utilization rate is increased, the cost is reduced, the yield per mu of dried arrow hemp products is187.2 kg each year, the output value is 42,000-66,000 yuan, compared with a traditional planting method, the yield per mu is 21,000-30,000 yuan higher, and the economic value is considerable.

The invention relates to the technical field of seedling growing, in particular to a seedling growing method of a woody plant. The seedling growing method comprises the steps of grafting a middle stock onto a base stock; when a seedling body on the middle stock begins sprouting, placing the plant into a ventilated and dark environment; when a new shoot grow to 250-260 mm, moving the plant out of the ventilated and dark environment, conducting injury treatment on the base part of the middle stock, and using hormone at the wound to accelerate rooting; grafting a scion onto the middle stock, and placing the plant in a greenhouse after grafting; when the grown height of the new shoot of the scion is over 50 mm, separating the plant and the base stock, wherein the separation point is located at the position above the grafting point of the base stock and the middle stock and below the wound of the injury treatment, so that the grafting seedling is obtained. According to the seedling growing method of the woody plant, a double-grafting technology and a separation cultivation method are combined, a large number of nursery stocks can be quickly obtained, and time used for raising the seedlings is reduced. The seedling growing method of the woody plant belongs to plant asexual reproduction, the stability of the quality and the purity of the seed seedling are guaranteed, and the industrialized seedling production and commercialized construction of nursery stocks are accelerated.