42 results about "Asexual reproduction" patented technology

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

The application provides a hard branch cutting breeding method of Actinidia kolomikta and Zizyphus jujuba, relates to the technical field of asexual reproduction and seedling raising, and comprises the following steps: (1) collecting 1-2 year old branches and pruning branch tips; (2) cutting into 10cm cuttings with more than two bud points, with the upper end of the cutting being inclined and the lower end being horizontal, and soaking the cutting in carbendazim for sterilization treatment; (3) selecting grass charcoal mixed with perlite as a cutting substrate, disinfecting the substrate after being loaded into a plug tray; (4) preparing a rooting agent, treating the cutting of step (2), inserting the cutting into the substrate of the plug tray, fixing the cutting, pouring carbendazim through the substrate, and covering the cutting with a transparent cover; (5) placing the cutting under the condition of 70% shading, increasing 20% illumination after new leaves are unfolded, controlling the temperature to be 10-30 DEG C, timely watering the cutting after cutting, combining with carbendazim sterilization, gradually opening the transparent cover after 40d of cutting, watering according to the situation, and spraying foliar fertilizer every 10d; and (6) transplanting and planting the cutting after 60d of cutting. The application has the beneficial effects of simplifying the hard branch cutting steps of Actinidia kolomikta and Zizyphus jujuba and shortening the cutting breeding period.

The invention relates to the technical field of traditional Chinese medicinal material planting. The invention provides a container confinement cultivation method for relieving asexual propagation continuous cropping obstacles of gastrodia elata, which comprises the following steps: (1) filling and paving planting soil in a container, placing wood sections and armillaria mellea, then earthing, carrying out heat preservation treatment, and culturing to obtain a fungus bed; and (2) exposing the fungus material, placing gastrodia elata seeds around the fungus material, covering soil, carrying out heat preservation treatment, and carrying out conventional planting management. By means of the cultivation method, centralized and standardized cultivation of the gastrodia elata can be promoted, the problem that suitable cultivation sites are gradually reduced in the gastrodia elata cultivation process is solved, the problem of continuous cropping obstacles of the gastrodia elata is effectively solved, and sustainable development of the gastrodia elata is promoted.

The invention provides a red and blue lycoris radiata tissue culture method, and relates to the technical field of plant propagation, the method sequentially comprises the steps of explant collection and pretreatment, explant disinfection, induction culture, multiplication culture, strong seedling culture and rooting culture, aiming at different requirements of each culture stage, a low-nitrogen / high-nitrogen adaptive culture medium system is customized, and the culture medium system is used for culturing the red and blue lycoris radiata. According to the method, the hormone proportion is accurately regulated and controlled, functional components such as acidolysis casein, coconut juice and activated carbon are added, meanwhile, culture environment parameters are optimized, an asexual propagation mode is adopted, efficient propagation of the lycoris radiata buds can be rapidly achieved, the problem of activity decline after long-term propagation is solved, the risks of pollution, browning and vitrification are effectively reduced, and the survival rate of the lycoris radiata buds is increased. The robustness and transplanting survival rate of tissue culture seedlings are improved, and meanwhile, stable inheritance of excellent characters of varieties is guaranteed.

This invention discloses the agronomic traits, InDel-labeled fingerprint, and construction method of the *Dictyophora indicum* strain YZS021, belonging to the field of *Dictyophora indicum* detection technology. The fingerprint is obtained by PCR amplification and electrophoresis detection using 10 pairs of InDel-labeled primers developed based on the whole genome of *Dictyophora indicum*. The InDel marker fingerprint spectrum detection of the *Dictyophora indicum* strain YZS021 of this invention is fast, accurate, and reproducible, and can specifically identify the strain, enabling precise identification of the fungal species and protection of intellectual property rights. In addition, this invention also discloses the agronomic traits of the *Dictyophora indicum* strain YZS021. The *Dictyophora indicum* strain 'YZS021' has a long and thick stipe, a high edible ratio, and good commercial characteristics. It can stably produce fruiting under both forest and facility cultivation modes, adapting to different cultivation scenarios and significantly improving planting efficiency. The above agronomic traits have consistency and stability, and can stably cultivate and obtain asexual reproduction lines with the above traits in both forest and facility fruiting rooms.

This invention relates to a rooting agent and seedling cultivation method for promoting rooting of *Cyclocarya paliurus* cuttings, belonging to the field of plant asexual reproduction technology. It solves one of the problems in existing *Cyclocarya paliurus* seedling cultivation: low rooting rate, long rooting time, few and poor-quality adventitious roots, and low transplant survival rate. This invention discloses a rooting agent for promoting rooting of *Cyclocarya paliurus* cuttings, which consists of two components, A and B. Component A consists of potassium indolebutyrate, soluble starch, chlorogenic acid, salicylic acid, and talc; component B consists of indolebutyric acid, sucrose, chlorogenic acid, vitamin C, and talc. Using the above-mentioned rooting agent and the corresponding seedling cultivation method, rapid rooting of *Cyclocarya paliurus* cuttings is achieved, with a rooting rate as high as 93.06%, and a large number and good quality of adventitious roots, resulting in a transplant survival rate of 90.37%.

The invention discloses a cutting propagation method for sinojackia xylocarpa, and belongs to the technical field of plant asexual propagation. The cutting propagation method of the sinojackia xylocarpa comprises the following steps: immersing cutting slips of the sinojackia xylocarpa into an ethylicin solution, and then inserting the cutting slips into a cutting medium for cultivation. By means of the cutting propagation method, the cutting rooting rate of the twigs of the sinojackia xylocarpa can be remarkably increased by controlling the cutting time, the cutting environment, the cutting treatment, the cutting medium and other factors, the highest cutting rooting rate after two months can reach 90%, and the highest cutting rooting rate after three months can reach 80% or above. The cutting propagation method provided by the invention is simple and rapid, low in cost and easy to implement on a large scale.

The invention belongs to the technical field of fruit cultivation, and particularly relates to an avocado rootstock asexual propagation method which comprises the following specific steps: material preparation, grafting and yellowing treatment, cup sleeving of a micro container and rooting promotion, rooting inspection, scion grafting, cutting and independent seedling hardening, and outplanting and transplanting. Compared with vegetative propagation, seedling propagation and tissue culture, the method has the advantages of high efficiency, multi-bud synchronization, high sapling consistency, root system and crown balance, full play of genetic potential, low transportation cost, high quarantine safety, good root system quality, high field planting survival rate and no rejuvenation period, and is suitable for large-scale, commercial, long-distance transportation and facility cultivation.

This invention discloses a species of Armillaria mellea ZM51, whose classification name is [not specified]. French Armillaria This strain was deposited on May 8, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.42001, located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. *Armillaria galbana* ZM51 exhibits unique characteristics such as rapid mycelial germination, strong resistance to contamination, and early fruiting. Through multiple generations of asexual reproduction, its traits remain stable and consistent. Compared to the existing *Armillaria olfleurone* 'Hazelnut-4', its mycelial germination time is shortened by 3-6 days, and fruiting occurs approximately 20-25 days earlier. In Liaoning province, through semi-wild cultivation, it achieves autumn fruiting after spring sowing, with clustered fruiting bodies, significantly larger mushrooms than 'Hazelnut-4', thicker stipes, and larger caps, demonstrating significantly enhanced resistance to contamination.

The invention relates to the technical field of short-petal rose cultivation, discloses a short-petal rose cultivation method and aims to stably cultivate short-petal roses. The method comprises the following steps: selecting a land parcel in which rosaceae and Chinese rose plants are not planted in recent three years, deeply ploughing, applying a base fertilizer, disinfecting by solar energy, and ventilating for later use; a greenhouse is built in a standby land parcel, parallel ridge bodies with the ridge spacing of 0.6-0.8 m and the ridge height of 25-35 cm are arranged in the greenhouse, a drip irrigation system is arranged, and the ridge surfaces and furrows are covered with grass-proof cloth; the method comprises the following steps: taking more than 6 generations of asexually propagated 1-year-old short-petal rose stock plant stems, disinfecting and promoting roots to obtain cutting seedlings, planting the cutting seedlings at 15-25 DEG C according to the row spacing of 0.4-0.6 m, and recovering the seedlings; performing timed drip irrigation, periodic fertilization, branch pruning and pest control on the short-petal rose young plants after seedling recovery to obtain short-petal rose adult plants. The method is standard in operation, capable of stably cultivating the short-petal roses and suitable for large-scale cultivation.

The invention relates to the technical field of plant breeding, and discloses an oriental cherry rhododendron cutting seedling raising method and application thereof.The method comprises the steps of S1, facility and substrate preparation, specifically, seedling raising is conducted in a greenhouse, and Kanuma soil is selected as a cutting substrate; s2, cutting preparation: from mid-to-late May to early-to-mid September, selecting annual branches with semi-lignified upper parts and no plant diseases and insect pests of a rhododendron simsii plant as cuttings, cutting the cuttings into cuttings with the length of 10-15cm, reserving leaves on the cuttings, performing cutting seedling raising of the rhododendron simsii in a greenhouse, preparing an advanced monitoring system and a control system, and performing cutting seedling raising of the rhododendron simsii in the greenhouse. Air temperature and humidity, soil temperature and humidity and illumination intensity can be accurately monitored in real time, the cuttage method provides an effective way for vegetative propagation, excellent characters of stock plants can be reserved, meanwhile, direct digging of wild oriental cherry rhododendron in the cold highland area can be reduced through manual cuttage propagation, local fragile ecological balance can be maintained, and the survival rate of the wild oriental cherry rhododendron in the cold highland area is increased. And a virtuous circle of protection and utilization is realized.

The invention discloses an application of rice OsTIP2; the invention discloses application of the gene 1, 2 in improving the resistance of rice to acetochlor and a rice breeding material thereof. The method comprises the following steps: knocking out OsTIP2; according to the OsTIP2 gene, the resistance of rice to acetochlor can be improved; the amino acid sequence of the gene 1, 2 is as shown in SEQ ID NO: 2; and by matching with acetochlor for use, simplified planting of the rice can be assisted, and important economic value is achieved. The herbicide resistance characteristic of rice edited by the gene can be stably inherited through sexual or asexual reproduction, and the gene can be used for constructing resistant rice germplasm resources.

The invention discloses a high-quality tomato inbred line breeding method based on wolfberry fruit eccDNAs transfer, and belongs to the field of plant biological breeding and traditional breeding, the method comprises the following steps: grafting by using tomato as a scion and wolfberry fruit as a rootstock, carrying out soil burying overwintering cultivation to induce the scion above a graft union to form a regenerated adventitious bud, and carrying out high-quality tomato inbred line breeding. Taking regenerated adventitious buds for asexual propagation, then performing continuous selfing, screening selfing lines with superior characters based on fruit morphology, physiological characteristics and quality indexes, and performing wolfberry eccDNAs verification on second-generation selfing plants. The high-quality flavor tomato inbred line with genetic stability is obtained by optimizing the breeding method, a foundation is created for research and development of new varieties, and the method has good popularization and application prospects.

The invention relates to the technical field of vegetative propagation of plants, and particularly discloses a method for promoting growth of a peony high-altitude layering propagation root system by using a humic acid-rich fertilizer solution, and the method adopts a humic acid-containing water-soluble fertilizer to promote rooting. In order to solve the problems of low propagation coefficient, complicated operation, high labor cost and the like of a traditional peony propagation method, especially a high-altitude layering propagation method, and the risk of hormone rooting agent residue, a humic acid-containing water-soluble fertilizer is innovatively adopted as a root promoting agent to smear girdling cuts below mixed buds to promote rooting. The method solves the technical bottlenecks of few induced root systems and slow growth in high-altitude layering propagation of the peony, is suitable for commercial seedling culture of the peony, protection of endangered wild species, propagation of hybrid superior plants and the like, and provides technical support for efficient propagation of the peony.

The invention belongs to the technical field of plant asexual propagation, and particularly relates to a bougainvillea speetabilis cutting propagation method. According to the method, current-year-old semi-lignified branches on the periphery of a bougainvillea speetabilis crown are cut as cutting slips, after the bases of the cutting slips are soaked with an indoleacetic acid solution with the mass concentration of 200 mg / L, the cutting slips are inserted into a yellow subsoil matrix in a cuttage mode, and the cutting slips are compacted, watered thoroughly and maintained every day. Cuttage seedlings obtained through rooting culture for 45 days can achieve core indexes that the number of roots is larger than or equal to 14, the longest root length is larger than or equal to 6.58 cm, the root water content is larger than or equal to 71.61%, the number of sprouts is larger than or equal to 3, and carotenoid is larger than or equal to 2.66 mg / g. According to the method, the problem of shortage of lignified cutting slips in bougainvillea speetabilis cutting propagation is solved, the problem of character variation is solved, the seedling raising cost is remarkably reduced through a standardized process, and efficient and reliable technical support is provided for large-scale production of bougainvillea speetabilis.

The present invention discloses a method for monitoring the asexual reproduction and growth of rhizomes of bluegrass based on clustering processing, and the method includes multi-source monitoring data acquisition, multi-source data preprocessing, construction of a growth status monitoring clustering model, clustering structure optimization and hierarchical clustering growth status monitoring. The present invention relates to the technical field of plant reproduction data processing, and specifically refers to a method for monitoring the asexual reproduction and growth of rhizomes of bluegrass based on clustering processing. This solution innovatively proposes a dual clustering mechanism based on a master-sub hierarchical structure, which significantly improves the accuracy, stability and agricultural usability of growth status monitoring; designs a misclassification point recognition mechanism, a misclassification trigger mechanism and introduces a cluster adjustment factor to enhance the adaptability and expression ability of complex plant growth monitoring behavior patterns; adopts a mirror inversion mechanism and a sine-cosine function method to improve the optimization algorithm, the optimal parameter combination of the growth status monitoring clustering model, optimizes the clustering structure, and improves the accuracy of the growth status monitoring results.

The application provides a breeding method and application of a young plant of a cow ear. The application adopts a leaf of the cow ear as an explant, optimizes a breeding technical system through induction of germination of double-face clustered buds of the leaf, and obtains a large amount of high-quality young plants. The method can overcome physiological time limitation of plant material taking, and will not affect species quantity and reproduction. Through induction of the double-face clustered buds of the leaf, a new way of asexual reproduction of the plant leaf is explored, utilization efficiency of the explant of the plant tissue culture is increased, and a simple, rapid and effective breeding system of the cow ear is established.

The invention relates to a group of SNP (Single Nucleotide Polymorphism) molecular markers associated with surface area characters of poplar roots and application, and belongs to the technical field of genetic breeding of poplar. The method comprises the following steps: identifying key sites influencing the root surface area through whole genome association analysis, and respectively positioning the key sites at the 10391007bp (SAsite1) and the 10391759bp (SAsite2) on the No.6 chromosome of a populus sibirica genome; the two sites are completely linked, and the root surface area of the heterozygous haplotype (GA / CG) is obviously larger than that of the homozygous haplotype (GG / CC). The molecular marker provided by the invention can be used for early molecular auxiliary selection of the surface area of the root of the poplar, is beneficial to accurately and efficiently screening a poplar clone with a relatively large root surface area, and provides reliable information for improving the survival rate of vegetative propagation and breeding an excellent strain.

The application belongs to the technical field of plant tissue culture and particularly relates to a tissue culture propagation method of Cupressus gigantea. In order to solve the technical problems of difficult asexual reproduction, low seed germination and rooting rates of Cupressus gigantea, the application provides the tissue culture propagation method of Cupressus gigantea, which comprises the following steps: taking Cupressus gigantea seeds or branches as explants, sterilizing the explants, and then sequentially performing initiation culture and rooting culture; when the Cupressus gigantea seeds are taken as the explants, the initiation culture further comprises proliferation culture. The application obtains the optimal tissue culture condition of Cupressus gigantea by screening the sterilization mode, the initiation culture medium, the proliferation culture medium and the rooting culture medium, and the survival rate of transplanting is more than 90% after seedling transplanting and local transplanting. The application provides technical support for the mass propagation of Cupressus gigantea and has important significance for the field return and population recovery of Cupressus gigantea.

The present application relates to the technical field of plant asexual reproduction, and particularly discloses a cutting propagation method for Lindleya benzoe. The method comprises the following steps: pretreating the base of cuttings with an activating solution containing oligosaccharide signal molecules and active components of root exudates of Lindleya benzoe, then cutting the cuttings in a special induction matrix containing slow-release plant growth regulators, and performing programmed induction of dark culture and light culture; during the light culture stage, dilute solution of the activating solution is applied to the base of the cuttings as an auxiliary inducer. The present application integrates endogenous signal activation, slow-release continuous supply and phased synergistic induction, systematically simulates and strengthens the chemical biological microenvironment of natural rooting, effectively activates the endogenous root potential of the cuttings, and solves the technical problems of low rooting rate, poor root quality and weak transplanting adaptability in conventional methods, thereby realizing efficient and high-quality propagation of Lindleya benzoe cutting seedlings.

The invention belongs to the technical field of plant asexual propagation, and relates to a method for rapid propagation by utilizing triarrhena lutarioriparia stem tip tissue culture. The method comprises the following steps: (S1) cultivating triarrhena lutarioriparia stem segments with axillary buds into seedlings through cuttage, taking stem tips, and disinfecting the stem tips with a botanical disinfectant to obtain triarrhena lutarioriparia stem tip explants; (S2) inoculating the triarrhena lutarioriparia stem tip explant into a callus induction culture medium containing 10-20mg / L of tea polyphenol, and inducing to form a callus; (S3) cutting the callus, inoculating the cut callus to a differential medium containing 0.5-0.8 g / L of proline and 3-5g / L of trehalose, and differentiating to obtain triarrhena lutarioriparia differentiated seedlings; (S4) transferring the triarrhena lutarioriparia differentiated seedlings into a rooting culture medium to induce rooting, so as to obtain triarrhena lutarioriparia stem tip tissue culture seedlings. According to the method, the stem tips of the cutting seedlings serve as explants, a specific disinfection process and an optimized culture medium are comprehensively adopted, a stable and efficient triarrhena lutarioriparia rapid propagation system is constructed, the problems that traditional explants are limited by seasons, the seedling rate is low and the like are solved, and technical support is provided for industrial propagation of triarrhena lutarioriparia.

The application discloses a jellyfish polyploid induction method and application thereof, and belongs to the technical field of marine ornamental animal breeding. The jellyfish polyploid induction method takes the polyp stage of jellyfish as an induction object, and polyploid jellyfish is obtained by induction treatment through combination of colchicine and dimethyl sulfoxide. The method can obtain multiple polyploid new germplasms through one treatment under the use of a small amount of inducers. The obtained polyploid polyps can not only rapidly proliferate new individuals with polyploid characteristics through asexual reproduction, but also obtain jellyfish bodies with polyploid characteristics, such as fast growth speed, long life cycle, beautiful shape and large individual. Meanwhile, the method has the characteristics of simple operation and low cost. Therefore, the induction method has great application value and broad application prospect.

This invention belongs to the field of crop genetics and breeding technology, and particularly relates to a method for breeding large-fruited thornless prickly pears using thornless prickly pear germplasm resources. The method includes the following steps: screening key parents from the seedling progeny population of thornless prickly pear germplasm, from which individuals exhibiting the thornless trait can be isolated; germinating the seeds of the key parents' progeny until they show white sprouts, and then chemically mutagenesis using colchicine solution to obtain a first-generation mutant population; selecting mutant plants from the first-generation mutant population with completely thornless fruit and a single fruit weight of not less than 20 grams; and performing asexual reproduction on the mutant plants, verifying through multiple generations of cultivation to obtain varieties with stable thornless and large-fruit traits. This invention effectively solves the technical problem of small fruit size and difficulty in genetically fixing the thornless trait in breeding from thornless prickly pear germplasm resources, creating a new prickly pear germplasm with large fruit size, thornlessness, and stable traits.

The application provides a camellia oleifera autumn promotes autumn grafting fast seedling bud seedling grafting method, and belongs to the technical field of plant asexual reproduction.The camellia oleifera autumn promotes autumn grafting fast seedling bud seedling grafting method comprises the following steps: (1) selecting early-maturing camellia oleifera seeds in autumn, removing or breaking the seed coat, spraying a germination agent, sealing and germinating to obtain germinated seeds; (2) transferring the germinated seeds to a rootstock bed, cultivating until the hypocotyl is 7-9 cm long, and obtaining bud seedlings for grafting; (3) selecting camellia oleifera lignified summer shoots or autumn shoots as scions, and using the hypocotyl wedge grafting method to graft in late autumn, and transplanting the grafted seedlings on the same day.The grafting method can improve the survival rate, shorten the seedling cycle by half a year, effectively reduce the germination of rootstock root nematodes during the seedling stage, and effectively reduce the seedling cost.

The invention relates to the technical field of plant tissue culture and asexual propagation, and particularly discloses a Lanzhou lily tissue culture seedling and scale cuttage linkage rapid propagation method which comprises the following steps: S1, a tissue culture seedling cultivation step; according to the method, through staged gradient environment domestication, tissue culture seedlings gradually adapt to the external environment, and the transplanting survival rate can be stabilized at 95% or above and is remarkably superior to 72.0% of a traditional seedling exercising technology; according to the method, through staged gradient environment domestication, the tissue culture seedlings gradually adapt to the external environment, and the transplanting survival rate can be stabilized at 95% or above and is remarkably superior to 72.0% of a traditional seedling exercising technology; according to the method, the propagation coefficient is greatly increased, namely, the bulbus disc of one tissue culture seedling can induce tillering to form 3-8 scales, each scale can form a new seed ball after cuttage, and the comprehensive propagation coefficient reaches 5-8 times and is increased by 3-5 times compared with 1-2 times of a traditional method.

The present application relates to the field of asexual reproduction technology, and more particularly to a method for large-scale cultivation of seedlings by using pineapple waste stem in field. The method comprises the following steps: after harvesting of pineapple fruits, collecting healthy above-ground stems of pineapple, removing leaves and residual fruit stalks, and soaking the base of the stems in a composite pretreatment solution for 25-35 minutes; immersing the pretreated stems in an indole butyric acid solution for 8-15 seconds; ridging, ditching on the ridge surface, and inserting the stems into the ditch at an angle of 35-45 degrees with the bud eye facing upwards, with 2 / 3 of the length of the stems being buried in the soil and the remaining part being exposed to the ground; pouring water thoroughly after insertion, controlling the light intensity, maintaining the soil temperature at 20-30 DEG C, and culturing for 45-60 days; when the newly grown seedlings grow to 15-25 cm, gradually increasing the light intensity for 7-10 days of seedling hardening, and taking out the stems together with the base when the seedlings are transplanted, and obtaining the seedlings after separation. The present application converts waste stems into seedlings, and realizes in-situ resource utilization of waste.

The application belongs to the technical field of jellyfish prevention and control, and discloses application of DMX-5804 in preparation of a medicine for inhibiting budding reproduction of Aurelia sp.1. By inhibiting budding reproduction of A.coerulea hydrocladium, the outbreak of Aurelia sp.1 can be prevented and controlled, and the adverse effects on marine ecological environment and human coastal economic activities can be reduced. Through experimental verification, 1 μM DMX-5804 can reduce the number of hydrocladium buds without affecting the living state of A.coerulea hydrocladium, which indicates that the compound DMX-5804 can inhibit the asexual reproduction of Aurelia sp.1 without toxicity at this concentration. The application provides a new theory and method for controlling the number of jellyfish, preventing the outbreak of Aurelia sp.1, and studying the biological conservative cell proliferation strategy.

This invention discloses a convenient and rapid method for propagating kudzu seedlings from leaves. By employing specially designed cutting plastic devices one and two, combined with the method of this invention, the growth status of the seedlings can be monitored in a timely manner, and nutrients can be provided. This significantly shortens the seedling time (approximately 10 days faster than traditional cutting propagation) and effectively improves the survival rate of kudzu seedlings (far exceeding the traditional cutting survival rate, reaching 90.0%) and the efficiency of seedling cultivation, while reducing the cost of kudzu seedling cultivation. Furthermore, this invention utilizes asexual reproduction, producing kudzu plants of consistent quality with the parent plant.

The invention discloses application of an MdTPPD gene in regulation and control of adventitious root generation of apple, the nucleotide sequence of the MdTPPD gene is as shown in SEQ ID NO.1, and the amino acid sequence of the MdTPPD protein is as shown in SEQ ID NO.2. According to the invention, through a plant genetic engineering technology, a DNA fragment of a complete coding segment of the related MdTPPD gene is separated and cloned from Huahuo apple, the function of the gene is verified, and the application of the MdTPPD gene in regulation and control of adventitious root generation of apple is realized. The invention finds that overexpression of the MdTPPD gene can inhibit rooting of tomato, and interference of expression of the MdTPPD gene can promote adventitious root generation of apple, which is crucial to improvement of asexual propagation efficiency and stress resistance of apple industry, and provides an important way for stress-resistant breeding of plants.

This invention discloses a specific DNA fragment, primers, identification method, and application for sex identification in yellowfin tuna. The specific DNA fragment has the following gene sequence: male as shown in SEQ ID NO.1 and female as shown in SEQ ID NO.2. Primers were designed based on the specific DNA fragment sequence for PCR amplification and Sanger sequencing analysis. The genotype at position 219 of the male yellowfin tuna DNA fragment is T / G heterozygous, and at position 292 it is A / G heterozygous. The genotype at position 219 of the female yellowfin tuna DNA fragment is T / T homozygous, and at position 292 it is A / A homozygous. This invention successfully achieves non-lethal sex identification during the fry or juvenile stage, which is beneficial for the implementation of asexual reproduction and seedling selection in yellowfin tuna, and has significant practical value and application potential.