Technology for breeding heartleaf houttuynia herb seedlings

A technology for Houttuynia cordata and seedlings is applied in the fields of biotechnology and crop cultivation, and can solve the problems of large rhizome volume, field pests and diseases, and difficulty in disinfection and treatment.

Inactive Publication Date: 2010-12-01
HUAIHUA UNIV
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Problems solved by technology

Moreover, because the rhizomes are large in size, it is not easy to carry out disinfection treatment, so it is easy to bring the source of diseases and in

Method used

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  • Technology for breeding heartleaf houttuynia herb seedlings
  • Technology for breeding heartleaf houttuynia herb seedlings
  • Technology for breeding heartleaf houttuynia herb seedlings

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Experimental program
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Effect test

Embodiment

[0026] Embodiment: Houttuynia cordata seedling propagation technology comprises the steps:

[0027] 1. Seed collection: From June to October every year, collect mature seeds when the infructescence of Houttuynia cordata starts to turn black, and the infructosis seeds can fall when lightly flicked.

[0028] 2. Seed preservation: Dry the collected seeds in a cool (20-28°C) dry and ventilated place.

[0029] 3. Seed disinfection: sterilize the surface of the seeds with 70% ethanol for 30 seconds, 0.1% mercuric chloride for 8 minutes, and rinse 4-5 times with sterile water.

[0030] 4. Seed low-temperature treatment: put the sterilized seeds in a 10 ml centrifuge tube, add 0.1% (W / V) agarose solution that has been sterilized in the tube to disperse the seeds evenly, place it in 1 Treat at -4°C for about 7 days.

[0031] 5. Seed acceleration and germination: the seeds after the low temperature treatment are evenly sown on the sterilized MS basic medium (sucrose concentration is 3...

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Abstract

The invention discloses technology for breeding heartleaf houttuynia herb seedlings, and relates to the fields of biotechnology and crop cultivation. The technology comprises seed collection, seed storage, seed sterilization, seed low-temperature treatment, seed germination, seedling cultivation and other processes, wherein the mature and dry heartleaf houttuynia herb seeds are sterilized by 70 percent ethanol and 0.1 percent mercuric chloride, are washed by sterile water for 4 to 5 times, and are treated at low temperature of between 1 and 4 DEG C for 7 days; and the seeds are sowed on an MS minimal medium and are transplanted to a seed bed for culturing seedling 14 days after the seeds are germinated in an illumination incubator. The technology treats the heartleaf houttuynia herb seeds at low temperature, changes the physiological status of the seeds, and promotes the seeds to germinate and grow to form seedlings. The seed breeding method can also avoid the problem of plant diseases and insect pests due to the fact that insect sources of a stock plant are easily brought to the seedlings by rhizome asexual reproduction, and can reduce planting cost simultaneously.

Description

technical field [0001] The invention relates to the fields of biotechnology and crop cultivation, and refers to a tissue or general culture-based seedling propagation technology of Houttuynia cordata, which can be used for the scientific research of Houttuynia cordata and the production of seedlings in the cultivation and production of Houttuynia cordata. technical background [0002] Houttuynia cordata Thunb. is a common name of Houttuynia cordata Thunb., a perennial herb, which is a traditional wild vegetable and Chinese herbal medicine. It has been used in my country for more than 2,000 years. Houttuynia cordata and its products were recommended as one of the eight Chinese medicinal materials for the treatment of SARS in 2003, and listed as one of the three traditional Chinese medicinal materials for the prevention and treatment of highly pathogenic avian influenza in 2004. It is traditionally believed that Houttuynia cordata has the effects of clearing heat and detoxifyi...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01C1/00A01C1/08
Inventor 伍贤进刘选明彭帅张俭李胜华
Owner HUAIHUA UNIV
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