Soil activation method

Abstract

The invention belongs to the technical field of soil improvement, and particularly relates to a soil activation method. The soil activation method comprises the step of applying a Govff soil antidote into soil, wherein the Govff soil antidote comprises Govff soil antidote granules, Govff soil antidote powder and a Govff soil antidote aqueous solution. The invention solves the problems of reduced physical and chemical properties of soil, reduced grape quality and the like caused by the input and retention of major elements of nitrogen, phosphorus and potassium in the soil in the current Xichang Clenon grape cultivation process, and can improve the resistance of grape vines, promote the germination, flowering and fruit setting of grapes, enhance the root growth, enhance the root activity, increase the thickness and flexibility of leaves and improve the yield of the grapes. Meanwhile, the proportion of beneficial bacteria in soil is increased, soil micro-ecological balance is restored, the yield of grapes is increased, grape fruit cracking is reduced, the influence of grapes on resisting diseases, drought and other adverse environments is increased, and guarantee is provided for yield increase.

Description

Technical Field

[0001] The invention belongs to the technical field of soil improvement, and in particular relates to a soil activation method. Background Art

[0002] Xichang is known as the "Little Spring City". It belongs to the tropical plateau monsoon climate zone, with an average annual temperature of 17.2℃ and more than 2,400 hours of sunshine per year. It is an excellent area for late-maturing grape varieties. Among them, there are about 150,000 mu of Cresson grapes, accounting for 1 / 5 of the national Cresson planting area. The yield per unit area of ​​Xichang Cresson grapes can exceed 10,000 catties, and the quality is excellent, which is the highest in Sichuan and even in the country. In 2020, the annual output value of Xichang grapes reached 2.5 billion yuan, and grapes have become the largest fruit industry in Xichang.

[0003] The nitrogen, phosphorus and potassium content in the soil exceeds the range required for normal crop growth, which may lead to soil pollution and changes in the physical and chemical properties of the soil. The large-scale use of chemical fertilizers, especially acidic fertilizers, will lead to soil acidification. During the acidification process, the pH value of the soil decreases, which not only affects the normal growth of crops, but also may cause certain elements in the soil to become unusable, further affecting the nutrient absorption of crops and affecting the quality of fruits. On the other hand, chemical fertilizers contain a large amount of inorganic salts, and long-term use will lead to soil salinization. The salt content of salinized soil is too high, which will affect the normal growth of crops.

[0004] Therefore, how to activate the existing large amounts of elements in the soil through technical means to achieve the goal of saving fertilizer and improving the physical and chemical properties of the soil is a key issue currently facing grape cultivation in Xichang.

[0005] At present, the above problems can be regulated through water-fertilizer integration and root zone restriction cultivation technology. However, it is still a temporary solution and not a fundamental solution. For example, water-fertilizer integration technology can achieve precise regulation of water and fertilizer, which not only saves water and fertilizer input, but also reduces the upward movement of soil salt caused by water evaporation. However, it has no effect on the excessive content of a large number of elements in the soil and the improvement of soil physical and chemical properties.

[0006] The root restriction cultivation technology places the root system of fruit trees within a controllable range, and regulates the relationship between the vegetative growth and reproductive growth of the aboveground and underground parts by controlling the growth of the root system. However, it is not very effective in activating the existing macroelements in the soil and improving the physical and chemical properties of the soil. Summary of the invention

[0007] Based on the above technical background, the main purpose of the present invention is to provide a soil activation method to overcome the shortcomings of the prior art.

[0008] In order to achieve the above-mentioned invention object, the technical solution adopted by the present invention includes: The present invention provides a soil activation method, which comprises applying a Gefu soil detoxifier to the soil, wherein the Gefu soil detoxifier comprises a Gefu soil detoxifier granule, a Gefu soil detoxifier powder and a Gefu soil detoxifier aqueous solution; The Gefu soil detoxifier granules contain effective bacterial species Penicillium glabrata or Bacillus subtilis, and the content of effective bacterial species Penicillium glabrata or Bacillus subtilis is ≥ 100 million / g; The Gefu soil detoxifier powder contains an effective bacterial species of Penicillium glabrata, and the content of the effective bacterial species of Penicillium glabrata is ≥ 200 million / g; The Gefu soil detoxifying agent aqueous solution contains effective bacterial species Bacillus subtilis, and the content of effective bacterial species Bacillus subtilis is ≥ 200 million / mL.

[0009] Preferably, the soil activation method comprises: Applying Gruyere Soil Detox Granules or Gruyere Soil Detox Powder after the grapes are off the shelves; and / or, Drip irrigation of the soil with Geff Soil Detoxifier during the budding period; and / or, Drip irrigate the soil with Gefu soil detoxifier solution during the leaf expansion to flowering stage; and / or, Drip irrigate the soil with Geff Soil Detoxifier powder during the fruit expansion period; and / or, Drip irrigate the soil with Geff Soil Detoxifier during the veraison period; and / or, During the harvest period, drip irrigate the soil with Gefu Soil Detoxifier Powder.

[0010] More preferably, after the grapes are taken off the shelves, Gefu soil detoxifier granules or Gefu soil detoxifier powder are applied, and the application amount of Gefu soil detoxifier granules or Gefu soil detoxifier powder is 5-10kg / mu, and 50-200kg / mu of organic fertilizer is applied at the same time.

[0011] More preferably, the soil is drip-irrigated with Gefu soil detoxifier solution during the budding period, 300 to 500 ml per mu each time, and the drip irrigation is once a month.

[0012] More preferably, the soil is drip-irrigated with the Gefu soil detoxifier aqueous solution during the leaf expansion stage to the flowering spike stage, with 1 liter per mu of soil being drip-irrigated each time, with an interval of 15 days between each drip irrigation, for a total of 2 drip irrigations.

[0013] More preferably, during the fruit expansion period, the soil is drip-irrigated with the Gefu soil detoxifier powder, 5 to 10 kg per mu each time, and applied once.

[0014] More preferably, the soil is drip-irrigated with Gefu soil detoxifier aqueous solution during the color change period, with 500 to 1000 ml per mu each time, with an interval of 15 days between each drip irrigation, for a total of 2 drip irrigations.

[0015] More preferably, the soil is drip-irrigated with the Gefu soil detoxifier powder during the harvest period, with 5 to 10 kg / mu applied each time, and applied once.

[0016] The beneficial effects of the present invention are: (1) The soil activation method of the present invention solves the problems of reduced soil physical and chemical properties and reduced grape quality caused by the retention of nitrogen, phosphorus and potassium in the soil during the cultivation of Kelunsen grapes in Xichang by applying Gefu soil detoxifier granules or Gefu soil detoxifier powder to the soil or drip-irrigating Gefu soil detoxifier aqueous solution to the soil.

[0017] The soil-applied Gefu soil detoxifying agent granules or Gefu soil detoxifying agent powder added in the soil activation process of the present invention contain Penicillium glabrata or Bacillus subtilis. Bacillus bacteria and Penicillium fungi play a key role in the soil activation process.

[0018] (2) The soil activation method of the present invention can promote rooting, improve the resistance of grapevines, promote grape germination, flowering, and fruit setting, enhance root growth, enhance root activity, increase leaf thickness and flexibility, and at the same time increase the proportion of beneficial bacteria in the soil, restore the soil microecological balance, increase grape yield, reduce grape cracking, and increase grape resistance to diseases, drought and other adverse environmental influences, thereby providing a guarantee for increased production.

[0019] (3) The soil activation method of the present invention can alleviate soil compaction, activate existing elements in the soil, improve the physical and chemical properties of the soil, and increase fertilizer utilization. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The changes in the dominant fungal species at the phylum level in the soil after harvest in Examples 1 to 4 and Comparative Example 1 are shown; Figure 2 The differences in the dominant fungal species at the genus level in the soil after harvesting in Examples 1 to 4 and Comparative Example 1 are shown; Figure 3 The changes in the dominant bacterial species at the phylum level in the soil after harvest in Examples 1 to 4 and Comparative Example 1 are shown; Figure 4 The differences in the dominant bacterial species at the genus level in the soil after harvest between Examples 1 to 4 and Comparative Example 1 are shown. DETAILED DESCRIPTION

[0021] The present invention will be described in detail below, and the characteristics and advantages of the present invention will become clearer and more distinct with the description.

[0022] The invention provides a soil activation method, which comprises applying a Gfu soil detoxifier to the soil. The Gfu soil detoxifier comprises a Gfu soil detoxifier granule, a Gfu soil detoxifier powder and a Gfu soil detoxifier aqueous solution.

[0023] The Gefu soil detoxifier granules contain effective bacterial species Penicillium glabrata or Bacillus subtilis, and the content of effective bacterial species Penicillium glabrata or Bacillus subtilis is ≥ 100 million / gram (100 million / gram, i.e. 100 million strains per gram).

[0024] The Gefu soil detoxifier powder contains an effective bacterial species Penicillium light-saturated, and the content of the effective bacterial species Penicillium light-saturated is ≥ 200 million / gram.

[0025] The Gefu soil detoxifying agent aqueous solution contains effective bacterial species Bacillus subtilis, and the content of effective bacterial species Bacillus subtilis is ≥ 200 million / mL.

[0026] Microbial agents are a type of substance that contains a large number of beneficial live bacteria and a variety of natural active substances. The microorganisms in the agents can reproduce in the soil and are an effective way to scientifically regulate the soil and improve plant growth. They can increase the richness of microbial flora in the soil, improve soil enzyme activity, and provide a good rhizosphere microbial environment for crop growth, thereby increasing crop yields and improving fruit quality.

[0027] The described Gefu soil detoxifier granules also contain (organic matter, NPK (ternary compound fertilizer containing nitrogen, phosphorus and potassium) etc.).

[0028] The Gefu soil detoxifier powder also contains (organic matter, NPK, etc.).

[0029] The Gefu soil detoxifier aqueous solution also contains (organic matter, NPK, etc.).

[0030] According to the present invention, the soil activation method comprises: applying Gfu soil detoxifier granules or Gfu soil detoxifier powder after the grapes are taken off the shelves, drip irrigating the soil with Gfu ​​soil detoxifier water solution during the budding period, drip irrigating the soil with Gfu ​​soil detoxifier water solution during the leaf expansion period to the flowering spike period, drip irrigating the soil with Gfu ​​soil detoxifier powder during the fruit expansion period, drip irrigating the soil with Gfu ​​soil detoxifier water solution during the color change period, and drip irrigating the soil with Gfu ​​soil detoxifier powder during the harvest period.

[0031] According to a preferred embodiment of the present invention, the soil activation method comprises: After the grapes are off the shelves, apply Gefu Soil Detoxifier Granules or Gefu Soil Detoxifier Powder at a rate of 5-10kg / mu, and apply 50-200kg / mu of organic fertilizer at the same time. This can promote rooting, improve the resistance of grapevines, alleviate soil compaction, and improve fertilizer utilization.

[0032] During the budding period, drip irrigate the soil with Gefu soil detoxifier solution, 300-500 ml per mu each time, once a month. This can activate soil nutrients and promote root growth and flower bud differentiation.

[0033] During the leaf expansion period to the flowering period, drip irrigation of Gefu soil detoxifier solution is applied to the soil, 1 liter per mu each time, with an interval of 15 days between each drip irrigation, for a total of 2 drip irrigations. Activating soil nutrients can increase the fruit setting rate.

[0034] During the fruit expansion period, drip irrigate the soil with Gefu soil detoxifier powder, apply 5-10kg / mu each time, and apply once. This will promote nutrient activation and improve nutrient conversion and utilization.

[0035] During the color change period, drip irrigate the soil with Gefu soil detoxifier solution, 500-1000 ml per mu each time, 15 days apart each time, for a total of 2 times. Promote nutrient absorption and improve fruit quality.

[0036] During the harvest period, drip irrigate the soil with Gefu soil detoxifier powder, apply 5-10kg / mu each time, and apply once. Increase tree nutrition and prevent premature aging and related diseases.

[0037] According to a further preferred embodiment of the present invention, the soil activation method comprises: After the grapes are removed from the shelves, apply the Gefu soil detoxifier granules at a rate of 5-10kg / mu. Mix well with organic fertilizer and other accumulated fertilizers and apply in trenches.

[0038] Germination period: mainly focus on activating the soil, raising ground temperature, awakening roots, promoting uniform and strong spore germination, and preventing nutrient deficiency symptoms such as yellowing and shrinkage of leaves.

[0039] Apply 2.5kg / mu of Puzilu (complex amino acid... 50g / L trace elements Zn+B+Mn+Mo... 4g / L) produced by Shanghai Hanhe Biotechnology Co., Ltd. and 2-2.5kg / mu of Puzisu (nitrogen N 12.0%, calcium oxide CaO 15.0%, magnesium oxide MgO 5.0%, trace elements (B+Zn+Fe+Mn+Mo) 0.6%) produced by Shanghai Hanhe Biotechnology Co., Ltd. to the soil, and use it 1-2 times every 10-15 days. At the same time, choose Gefu soil detoxifier water to drip irrigate the soil, 300-500 ml per mu each time, and drip irrigate once a month.

[0040] Leaf expansion stage - flower spike stage: rooting, seedling promotion, shoot promotion, leaf growth, growth stimulation, relief of dead seedlings, promotion of flower bud differentiation, promotion of flower spike elongation, nitrogen and phosphorus release; at the same time, prevention of damage caused by thrips, aphids, hanging wireworms, cotton borer, and fall armyworm.

[0041] During the leaf expansion period to the flowering period, apply 2.5-3.5kg of Puzilan from Shanghai Hanhe Biotechnology Co., Ltd. + 500ml / mu of Enosan from Shikem Crop Science (Wuxi) Co., Ltd., twice with an interval of 10-15 days, 2.5-5kg / mu of diammonium phosphate, 5kg / mu of Puziling from Shanghai Hanhe Biotechnology Co., Ltd., once; spray 25% pyraclostrobin 10g / mu + 26% pyrimethanil·ethionyl 20g / mu + 30% tolfenpyrad 8ml / mu + Yinglai (titanium ≥2.5%, magnesium ≥10%, Qingdao Fushuo Agricultural Technology Co., Ltd.) 4g / mu on the leaves, once every 7 days. It can not only prevent aphids, thrips and leafhoppers, but also promote root development, make the plants grow vigorously and neatly, have large leaves, good flowering spikes, and improve the ability to resist adverse climates such as frost. At the same time, choose Gefu soil detoxifier water to drip irrigate the soil, 1 liter per mu each time, drip irrigate every 15 days, and drip irrigate twice in total.

[0042] Fruit expansion period: Promote rapid fruit expansion, increase the thickness and toughness of the fruit skin, prevent fruit cracking, improve photosynthesis, and prevent magnesium deficiency and yellowing of leaves in the later stage. At the same time, prevent black pox, brown spot, powdery mildew and thrips damage, grape sunburn (sun fruit), fruit cracking, etc.

[0043] Apply balanced compound fertilizer 15-15-15 (40kg / mu) + Shanghai Hanhe Biotechnology Co., Ltd. Puzi magnesium 5-10kg / mu + Puzi color balanced type 5kg / mu + Puzi blue 5-7.5kg / mu, bury once; spray French Gaomoer Hailijia foliar fertilizer 30ml / mu + 26% pyrimethanil·dithiocarb 20g / mu + 30% bifenazate tetraclofenazine suspension 10g / mu + 45% chlorfenapyr·lufenuron 5g / mu, spray once. It can effectively promote the absorption of trace elements such as calcium and magnesium, reduce grape cracking, improve grape resistance, increase calcium absorption and utilization, and increase grape soluble solids and sugar content. At the same time, choose Gefu soil detoxifier powder to drip irrigate the soil, apply 5~10kg / mu per mu each time, and apply once.

[0044] Color change period: Promote rapid coloring of fruits, promote the synthesis and transportation of sugars, increase fruit powder, improve fruit hardness, improve fruit quality, and prevent premature aging. At the same time, prevent downy mildew, powdery mildew, anthracnose, white rot, gray mold, and brown spot.

[0045] Apply high potassium compound fertilizer 5kg / mu + balanced compound fertilizer 5-7.5-10kg / mu + Shanghai Hanhe Biotechnology Co., Ltd. Puziling 5kg / mu + Shanghai Hanhe Biotechnology Co., Ltd. Puzisu 2-3kg / miao, once every 10-15 days; Mercay Jinshikai 43% tebuconazole 6ml / mu + 40% enoyl quinoline copper 15g / mu + 45% pyraclostrobin methyl thiophanate 12.5g / mu to prevent various diseases of grapes in the later stage and protect grapes more comprehensively. At the same time, choose Gefu soil detoxifier water to drip irrigate the soil, 500-1000 ml per mu each time, 15 days apart each time, for a total of 2 drip irrigations.

[0046] Harvest period: Prevent downy mildew from damaging old leaves and causing leaf fall and premature aging.

[0047] Apply 5kg / mu of Puzilu or Puziling produced by Shanghai Hanhe Biotechnology Co., Ltd., spray 5g / mu of 75% tebuconazole·pyraclostrobin + 15g / mu of 6% kasugamycin, prevent diseases and keep leaves open in autumn, increase tree nutrients, reduce the number of pathogens, and have fewer diseases, better flower buds, and higher yields in the next year. At the same time, drip irrigate the soil with Gefu soil detoxifier powder during the harvest period, apply 5-10kg / mu per mu each time, and apply once.

[0048] Example The present invention is further described below by means of specific examples. These embodiments are only intended to illustrate the present invention but are not intended to limit the scope of the present invention.

[0049] The following examples and comparative examples of the present invention were set up at the production base of Sichuan Yuecheng Pearl Agriculture Co., Ltd. in Anning Town, Xichang City. The test plot was selected as District 2 9#, with a plot area of ​​12.4 mu, a total of 30 compartments, and about 20 plants per compartment. The grape variety tested was Sunshine Rose, a three-year seedling. This test was set up with 5 treatments, namely, Example 1, Example 2, Example 3, Example 4 and Comparative Example 1, each treatment had 6 compartments.

[0050] The Gefu soil detoxifier used in the embodiment of the present invention includes granules, powders and aqueous solutions, as follows: Gefu soil detoxifier (granules, effective strains of Penicillium saturatum / Bacillus subtilis ≥ 100 million / g); Gefu soil detoxifier (powder, effective strain of Penicillium leucoderma ≥ 200 million / g); Gefu soil detoxifier (aqueous solution, effective bacteria species Bacillus subtilis ≥ 200 million / mL).

[0051] Example 1 After the grapes are taken off the shelves, apply the Gefu soil detoxifier granules at a rate of 5-10kg / mu, mix well with organic fertilizer and other accumulated fertilizers (the application rate of organic fertilizer and other accumulated fertilizers is the same as conventional fertilization, the same below), and then apply in trenches.

[0052] Germination period: mainly focus on activating the soil, raising ground temperature, awakening roots, promoting uniform and strong spore germination, and preventing nutrient deficiency symptoms such as yellowing and shrinkage of leaves.

[0053] Apply 2.5kg / mu of Puzilu (complex amino acid... 50g / L trace elements Zn+B+Mn+Mo... 4g / L) produced by Shanghai Hanhe Biotechnology Co., Ltd. and 2-2.5kg / mu of Puzisu (nitrogen N 12.0%, calcium oxide CaO 15.0%, magnesium oxide MgO 5.0%, trace elements (B+Zn+Fe+Mn+Mo) 0.6%) produced by Shanghai Hanhe Biotechnology Co., Ltd. to the soil, and use it 1-2 times every 10-15 days. At the same time, choose Gefu soil detoxifier water to drip irrigate the soil, 300-500 ml per mu each time, and drip irrigate once a month.

[0054] Leaf expansion stage - flower spike stage: rooting, seedling promotion, shoot promotion, leaf growth, growth stimulation, relief of dead seedlings, promotion of flower bud differentiation, promotion of flower spike elongation, nitrogen and phosphorus release; at the same time, prevention of damage caused by thrips, aphids, hanging wireworms, cotton borer, and fall armyworm.

[0055] During the leaf expansion period to the flowering period, apply 2.5-3.5kg of Puzilan from Shanghai Hanhe Biotechnology Co., Ltd. + 500ml / mu of Enosan from Shikem Crop Science (Wuxi) Co., Ltd., twice with an interval of 10-15 days, 2.5-5kg / mu of diammonium phosphate, 5kg / mu of Puziling from Shanghai Hanhe Biotechnology Co., Ltd., once; spray 25% pyraclostrobin 10g / mu + 26% pyrimethanil·ethionyl 20g / mu + 30% tolfenpyrad 8ml / mu + Yinglai (titanium ≥2.5%, magnesium ≥10%, Qingdao Fushuo Agricultural Technology Co., Ltd.) 4g / mu on the leaves, once every 7 days. It can not only prevent aphids, thrips and leafhoppers, but also promote root development, make the plants grow vigorously and neatly, with large leaves and good flowering spikes, and improve the ability to resist adverse climates such as frost. At the same time, the Gefu soil detoxifier aqueous solution is drip-irrigated to the soil, 1 liter per mu each time, 15 days apart each time, for a total of 2 drip irrigations.

[0056] Fruit expansion period: Promote rapid fruit expansion, increase the thickness and toughness of the fruit skin, prevent fruit cracking, improve photosynthesis, and prevent magnesium deficiency and yellowing of leaves in the later stage. At the same time, prevent black pox, brown spot, powdery mildew and thrips damage, grape sunburn (sun fruit), fruit cracking, etc.

[0057] Apply balanced compound fertilizer 15-15-15 (40kg / mu) + Shanghai Hanhe Biotechnology Co., Ltd. Puzi magnesium 5-10kg / mu + Puzi color balanced type 5kg / mu + Puzi blue 5-7.5kg / mu, bury once; spray French Gaomoer Hailijia foliar fertilizer 30ml / mu + 26% pyrimethanil·dithiocarb 20g / mu + 30% bifenazate tetraclofenazine suspension 10g / mu + 45% chlorfenapyr·lufenuron 5g / mu, spray once. , can effectively promote the absorption of trace elements such as calcium and magnesium, reduce grape cracking, improve grape resistance, increase calcium absorption and utilization rate, and increase grape soluble solids and sugar content. At the same time, choose Gefu soil detoxifier powder to drip irrigate the soil, apply 5~10kg / mu per mu each time, and apply once.

[0058] Color change period: promotes rapid coloring of fruits, promotes the synthesis and transportation of sugars, increases fruit powder, improves fruit hardness, improves fruit quality, and prevents premature aging. At the same time, it prevents downy mildew, powdery mildew, anthracnose, white rot, gray mold, and brown spot.

[0059] Apply high potassium compound fertilizer 5kg / mu + balanced compound fertilizer 5-7.5-10kg / mu + Shanghai Hanhe Biotechnology Co., Ltd. Puziling 5kg / mu + Shanghai Hanhe Biotechnology Co., Ltd. Puzisu 2-3kg / miao, once every 10-15 days; Mercay Jinshikai 43% tebuconazole 6ml / mu + 40% enoyl quinoline copper 15g / mu + 45% pyraclostrobin methyl thiophanate 12.5g / mu to prevent various diseases of grapes in the later stage and protect grapes more comprehensively. At the same time, choose Gefu soil detoxifier water to drip irrigate the soil, 500-1000 ml per mu each time, 15 days apart each time, for a total of 2 drip irrigations.

[0060] During the harvest period, the main focus is to prevent downy mildew from damaging old leaves and causing leaf fall and premature aging.

[0061] Apply 5kg / mu of Puzilu or Puziling produced by Shanghai Hanhe Biotechnology Co., Ltd., spray 5g / mu of 75% tebuconazole·pyraclostrobin + 15g / mu of 6% kasugamycin, prevent diseases and keep leaves open in autumn, increase tree nutrients, reduce the number of pathogens, and have fewer diseases, better flower buds, and higher yields in the next year. At the same time, drip irrigate the soil with Gefu soil detoxifier powder during the harvest period, apply 5-10kg / mu per mu each time, and apply once.

[0062] Example 2 The soil was activated in a manner similar to that of Example 1, except that conventional fertilization was reduced by 20%, the Gefu soil detoxifier was applied during the grape growth cycle, the Gefu soil detoxifier granules were applied after the grapes were removed from the shelves, the Gefu soil detoxifier aqueous solution was applied during the budding period, and the Gefu soil detoxifier aqueous solution was applied during the leaf expansion period to the flowering spike period. Example 3 The soil was activated in a manner similar to that of Example 1, except that conventional fertilization was used, the Gefu soil detoxifier was applied during the grape growth cycle, and the Gefu soil detoxifier powder was applied after the grapes were removed from the shelves. The application amount of the powder was 5-10 kg / mu, and it was mixed with organic fertilizer and other accumulated fertilizers and then applied in trenches. The Gefu soil detoxifier aqueous solution was applied during the budding period, and the Gefu soil detoxifier aqueous solution was applied before the flowering period.

[0063] Example 4 The soil was activated in a manner similar to that of Example 1, except that conventional fertilization was reduced by 20%, the Gefu soil detoxifier was applied during the grape growth cycle, and the Gefu soil detoxifier powder was applied after the grapes were taken off the shelves. The application amount of the powder was 5-10 kg / mu, and it was mixed with organic fertilizer and other accumulated fertilizers and then applied in trenches. The Gefu soil detoxifier aqueous solution was applied during the budding period, and the Gefu soil detoxifier aqueous solution was applied before flowering. Comparative Example Comparative Example 1 The soil was activated in a manner similar to that of Example 1, except that no Gefu soil detoxifier was applied during the grape growing period.

[0064] Experimental example Experimental Example 1 Soil physical and chemical properties After one growth cycle, soil samples with a soil layer thickness of 0 to 30 cm near the root system of the grapes were collected by a five-point sampling method, and samples of about 1.0 kg were obtained by a quartering method (the samples were taken from Examples 1 to 4 and Comparative Example 1, respectively). The pH value, CEC value, organic matter, total nitrogen, hydrolyzable nitrogen, available phosphorus, available potassium, urease, sucrase, acid phosphatase, catalase and other content changes of the soil were detected, and the tests were shown in Tables 1 and 2. In Tables 1 and 2, CK represents Comparative Example 1, and S1, S2, S3, and S4 represent Example 1, Example 2, Example 3, and Example 4, respectively.

[0065] Table 1

[0066] Table 2

[0067] It can be seen from Table 1 and Table 2 that, compared with Comparative Example 1, the contents of organic matter, total nitrogen, hydrolyzable nitrogen, available phosphorus, available potassium, cation exchange capacity, pH value, catalase, sucrase and acid phosphatase in the soil collected in Examples 1-4 are all higher than the above indicators in the soil collected in Comparative Example 1. This shows that the soil activation method of the present invention can increase the contents of organic matter, total nitrogen, hydrolyzable nitrogen, available phosphorus, available potassium, cation exchange capacity, pH value, catalase, sucrase and acid phosphatase in the soil.

[0068] Experimental Example 2 Growth and Physiological Indicators of Grapes during the Growth Period Grape branches and leaves of Examples 1-4 and Comparative Example 1 were collected for index testing. Branch growth index: The length and thickness of the internodes of the new shoots were recorded during the grape maturity period.

[0069] Leaf index: A portable chlorophyll meter was used to measure the chlorophyll content and nitrogen content of the leaves. At the same time, the content of trace elements such as calcium, magnesium, and boron in the grape leaves was measured. The test results are shown in Table 3, in which CK represents comparative example 1, and S1, S2, S3, and S4 represent embodiment 1, embodiment 2, embodiment 3, and embodiment 4, respectively.

[0070] Table 3

[0071] As can be seen from Table 3, the internode thickness of the grape branches of Examples 1-4 is thicker than that of Comparative Example 1. The contents of trace elements such as calcium, magnesium, and boron in the grape leaves of Examples 1-4 are higher than those in the grape leaves of Comparative Example 1, and the chlorophyll and nitrogen contents of the grape leaves of Examples 1-4 are also higher than those in the grape leaves of Comparative Example 1. This indicates that the soil activation method of the present invention can not only increase the content of organic matter in the soil, but also increase the internode thickness of the grape branches, as well as the contents of trace elements such as calcium, magnesium, and boron, chlorophyll, and nitrogen in the grape leaves.

[0072] Experimental Example 3 Grape Yield Index During the grape fruit harvest period, the per-acre yield and per-plant yield in Examples 1-4 and Comparative Example 1 were measured, 15 medium-sized ears were randomly selected for each treatment, the ear weight was measured, and 100 grapes were randomly selected from the upper, middle and lower parts of the ear at a ratio of 1:1:1 to measure the 100-grain weight. The test results are shown in Table 4. In Table 4, CK represents Comparative Example 1, and S1, S2, S3, and S4 represent Example 1, Example 2, Example 3, and Example 4, respectively.

[0073] Table 4

[0074] It can be seen from Table 4 that the grape yields of Examples 1-4 are all higher than the grape yield of Comparative Example 1, indicating that the activation method of the present invention can increase the grape yield.

[0075] Experimental Example 4 Microbial indicators in the root zone of grapes During the harvest period, rhizosphere soil samples with densely distributed grape roots were collected from Example 1, Example 2, Example 3, Example 4, and Comparative Example 1. First, most of the clay attached to the roots was removed, and then the roots with soil were cut off with scissors and placed in a sterile tube containing sterile PBS. The attached rhizosphere soil was shaken off by shaking to serve as a sample for detecting rhizosphere microorganisms. One sample was collected with 1g of fresh soil, placed in a sterile tube for storage, placed in an ice box or thermos bottle in the field, and stored in a refrigerator at -20°C or -80°C as soon as possible after arriving in the laboratory. Each treatment was repeated 3 times. The diversity of soil microorganisms, such as the abundance and species of microorganisms such as bacteria and fungi, was detected and analyzed. In order to clarify the effect of biochar on the species composition of soil microorganisms, the changes in the dominant fungal species were analyzed at the phylum level, such as Figure 1 Based on the differences at the phylum level, the differences among the treatment groups were further analyzed at the genus level, as shown in Figure 2 To clarify the effect of biochar on soil microbial species composition, the changes in dominant bacterial species were analyzed at the phylum level, such as Figure 3 Based on the differences at the phylum level, the differences in the dominant bacterial species in each treatment group were further analyzed at the genus level. Figure 4 shown. Figure 1 to Figure 4 In the figure, CK represents comparative example 1, and S1, S2, S3, and S4 represent embodiment 1, embodiment 2, embodiment 3, and embodiment 4, respectively.

[0076] from Figure 1 It can be seen that the top 10 dominant phyla in the average relative abundance of fungi in each treatment group are Ascomycota, Basidiomycota, Rozellomycota, Mortierellomycota, Chytridiomycota, Glomeromycota, Zoopagomycota, Blastocladiomycota, Aphelidiomycota, and Kickxellomycota. The total average relative abundance accounts for more than 99%. The dominant species are consistent among the treatment groups, but there are certain differences in their proportions.

[0077] from Figure 2It can be seen that compared with the CK treatment group, the unclassified_o__Pleosporales, Botryotrichum and Fusarium fungi in the S1, S2, S3 and S4 treatment groups were significantly reduced, and the Fungi_gen_Incertae_sedis, Leucoagaricus and Penicillium fungi increased.

[0078] from Figure 3 It can be seen that the top 10 dominant phyla with the average relative abundance of bacteria in each treatment group are Bacteroidota, Patescibacteria, Proteobacteria, Firmicutes, Acidobacteriota, Verrucomicrobiota, Dependentiae, Chloroflexi, Bdellovibrionota, and Cyanobacteria. The average relative abundance accounts for 91.88% to 98.93%. The dominant species are consistent among the treatment groups, but there are certain differences in their proportions. In the S1 and S2 treatment groups, the Bacteroidota and Patescibacteria were significantly decreased, while the Proteobacteria, Firmicutes and Acidobacteriota were significantly increased; in the S3 and S4 treatment groups, the Bacteroidota and Patescibacteria based on the phylum level were significantly increased, while the Proteobacteria, Firmicutes and Acidobacteriota were significantly decreased.

[0079] from Figure 4 It can be seen that norank_f__LWQ8, norank_f__Chitinophagaceae, and norank_o__Saccharimonadales in the S1 and S2 treatment groups decreased significantly; norank_f__LWQ8 and norank_f__Chitinophagaceae in the S3 and S4 treatment groups increased significantly; and Bacillus bacteria in the S1, S2, S3, and S4 treatment groups increased significantly. This fully proves the key role of Bacillus bacteria and Penicillium fungi in soil activation.

[0080] The present invention has been described in detail above in conjunction with specific implementations and exemplary examples, but these descriptions cannot be understood as limiting the present invention. Those skilled in the art understand that, without departing from the spirit and scope of the present invention, a variety of equivalent substitutions, modifications or improvements may be made to the technical solution of the present invention and its implementation methods, all of which fall within the scope of the present invention. The scope of protection of the present invention shall be subject to the attached claims.

Claims

1. A soil activation method, characterized in that: The soil activation method comprises applying a Gefu soil detoxifier to the soil, wherein the Gefu soil detoxifier comprises a Gefu soil detoxifier granule, a Gefu soil detoxifier powder and a Gefu soil detoxifier aqueous solution; The Gefu soil detoxifier granules contain effective bacterial species Penicillium glabrata or Bacillus subtilis, and the content of effective bacterial species Penicillium glabrata and Bacillus subtilis is ≥ 100 million / g; The Gefu soil detoxifier powder contains an effective bacterial species of Penicillium glabrata, and the content of the effective bacterial species of Penicillium glabrata is ≥ 200 million / g; The Gefu soil detoxifying agent aqueous solution contains effective bacterial species Bacillus subtilis, and the content of effective bacterial species Bacillus subtilis is ≥ 200 million / mL.

2. The soil activation method according to claim 1, characterized in that: The soil activation method comprises: Apply Gauve Soil Detoxifier Granules or Gauve Soil Detoxifier Powder after the grapes are off the shelves, along with organic fertilizer; and / or, Drip irrigation of the soil with Geff Soil Detoxifier during the budding period; and / or, Drip irrigate the soil with Gefu soil detoxifier solution during the leaf expansion to flowering stage; and / or, Drip irrigate the soil with Geff Soil Detoxifier powder during the fruit expansion period; and / or, Drip irrigate the soil with Geff Soil Detoxifier during the veraison period; and / or, During the harvest period, drip irrigate the soil with Gefu Soil Detoxifier Powder.

3. The soil activation method according to claim 2, characterized in that: After the grapes are taken off the shelves, apply Gefu Soil Detoxifier Granules or Gefu Soil Detoxifier Powder. The application rate of Gefu Soil Detoxifier Granules or Gefu Soil Detoxifier Powder is 5-10kg / mu, and apply 50-200kg / mu of organic fertilizer at the same time.

4. The soil activation method according to claim 2, characterized in that: During the budding period, drip irrigate the soil with Gefu soil detoxifier solution, 300-500 ml per mu each time, and drip irrigate once a month.

5. The soil activation method according to claim 2, characterized in that: During the leaf expansion stage to the flowering stage, drip irrigate the soil with Gefu soil detoxifier solution, 1 liter per mu each time, with an interval of 15 days between each drip irrigation, for a total of 2 drip irrigations.

6. The soil activation method according to claim 2, characterized in that: During the fruit expansion period, drip irrigate the soil with Gefu soil detoxifier powder, apply 5-10kg / mu per mu each time, and apply once.

7. The soil activation method according to claim 2, characterized in that: During the color change period, drip irrigate the soil with Gefu soil detoxifier solution, 500-1000 ml per mu each time, with an interval of 15 days between each irrigation, for a total of 2 drip irrigations.

8. The soil activation method according to claim 2, characterized in that: During the harvest period, drip irrigate the soil with Gefu soil detoxifier powder, apply 5-10kg / mu per mu each time, and apply it once.

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