187 results about "Vitis vinifera" patented technology

The invention relates to ice wine yeast with high fermentation activity and outstanding aroma-producing characteristics and application thereof. The ice wine yeast is yeast (Saccharomyces cerevisiae) YSL16010, and the bacterial strain has been preserved in China General Microbiological Culture Collection Center on April 18, 2016, with a preservation number of CGMCC No.12349. The bacterial strain YSL16010 is obtained by separating and screening Vidal grapes from a Huanren producing area of Liaoning at a temperature of 16 DEG C in a natural fermentation process, and the ice wine wine-making yeast having good making characteristics and highly producing esters is obtained through a series of screening and identifications and small pilot scale fermentation. The ice wine wine-making yeast YSL1610 highly producing esters is used for fermenting and producing ice wine, so that the yield of volatile acid is effectively controlled while the standards of the grape wine producing countries can be met, and therefore, the content of aromatic compounds such as esters is remarkably increased. The bacterial strain can be applied to ice wine brewing industry, and can play an important role in improving the quality of the ice wine and bringing advantages of the grape producing areas into play.

The invention discloses an image processing-based intelligent detection method and system for the degree of a grape air burning disease, and the method comprises the steps: automatically collecting a visible light image of a grape plant, segmenting and recognizing a leaf and fruit region, extracting the color and texture features, and comparing the color and texture features with a pre-stored air burning disease symptom feature library, thereby recognizing an early damaged abnormal region. By calculating the proportion and morphological characteristics of affected areas, a single leaf affected index and a fruit affected grade are generated, the quantitative disease degree grade of the whole plant is obtained through comprehensive evaluation, and a disease development trend obtained through historical image sequence analysis and real-time temperature and humidity data collected by an environment sensor are fused. The early warning of the disease diffusion risk is realized, and the targeted grading farming operation suggestions are generated according to the disease grade, the spatial distribution characteristics and the prediction result. According to the invention, diagnosis and early warning of the grape air cautery are realized, the problems of lagging and strong subjectivity of traditional manual inspection are effectively overcome, and decision support is provided for accurate prevention and control.

The invention discloses a method for visually detecting grape black root bacteria Ilyonectria sp. Based on RPA-CRISPR / Cas12a and application of the method for visually detecting the grape black root bacteria Ilyonectria sp. Based on the RPA-CRISPR / Cas12a. According to the method, an RPA specific amplification primer, a CrRNA probe and an ssDNA probe are adopted; according to the RPA specific amplification primer, an HIS gene is used as a specific recognition target, and specific amplification can be carried out on the grape black root pathogen Ilyonectria. The method for detecting the grape black root pathogenic bacteria has high specificity and sensitivity, is simple, convenient and rapid in operation flow, and is suitable for rapid screening and preliminary diagnosis of target pathogens in a field environment or a basic detection scene.

The invention discloses a screening and dosage determining method of a grape heat resistance inducer, and belongs to the technical field of plant stress resistance physiology and cultivation. The method comprises the following steps: soaking grape leaf wafers in inducers with different concentrations, and carrying out heat treatment under a plurality of temperature gradients; determining a chlorophyll OJIP rapid fluorescence parameter and constructing a heat resistance comprehensive index, and further calculating a normalized relative heat resistance improvement value; fitting a quantitative relation with the concentration by using a single-peak dose-effect model containing a high-dose inhibition item; statistical inference is carried out by adopting Bootstrap resampling, and a stable and effective recommended application concentration in a wide temperature range is finally determined by analyzing a common intersection or a statistical median of effective concentration intervals under multiple temperatures. The method realizes indoor rapid and quantitative screening, can realize quantitative comparison of induction intensity and dose sensitivity of different inducers under a unified evaluation system, and can be used for identifying heat resistance of grapes.

A composition containing a Vitis vinifera leaf extract as an active ingredient and its uses in preventing or treating tinnitus are disclosed. The composition may be a pharmaceutical composition or a food composition. The composition containing a Vitis vinifera leaf extract as an active ingredient is excellent in alleviating and improving tinnitus caused by noise and the like, and therefore is very useful as a composition for preventing or treating tinnitus.

The invention relates to the technical field of wine preparation, and discloses a brewing method of low-degree sweet type red wine, the brewing method comprises ten steps of raw material screening, segmented temperature control extraction, compound fermentation, gradient degree reduction, low-temperature sugar stabilization, flavor blending, clarification and filtration, aging and curing, sterile filling and finished product inspection, the alcoholic strength of the final product is 8% vol to 11% vol, the residual sugar content is 45 g / L to 65 g / L, and the content of the low-degree sweet type red wine is 1%. The total acid content is 5.5 g / L-7.0 g / L. And raw material screening. According to the preparation method disclosed by the invention, through proportioning the cabernet sauvignon and the rosa rugosa as raw materials and combining segmented temperature-controlled extraction and compound fermentation, anthocyanin and fruity substances in the grape skin residues can be fully extracted, and multi-flavor esters can be generated by virtue of aroma-producing yeast; the natural crataegus pinnatifida bunge concentrated juice and the rose essential oil are subsequently added for blending, and oak barrel aging and tannase astringency reduction are matched, so that the finished product finally presents a compound flavor, is mellow and sweet in taste, free of astringency and long in aftertaste, and solves the problems that the traditional low-degree sweet red is thin in flavor and inharmonious in fragrance.

This invention belongs to the field of agricultural biotechnology, and specifically relates to a strain of *Dictyophora indica* Phd01, its inoculant, and its application. *Dictyophora indica* Phd01 is classified and named... Phallus dongsun The strain was deposited on January 23, 2026, at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 2026233, located at Wuhan University, Wuhan, China. The fermentation broth of this strain significantly inhibited the mycelial growth of *Botrytis cinerea* and *Anthracnose spp.*; it showed a relative control efficacy of 42.31% against gray mold in peppers and enhanced the oxygen free radical scavenging capacity of pepper fruits, effectively extending the storage time of pepper fruits; the relative control efficacy against anthracnose in strawberries reached 31.03–40.23%, and it prolonged the time of strawberry disease onset. This indicates that the fermentation broth of *Dendrobium nobile* Phd01 can significantly inhibit strawberry and pepper rot, thereby extending shelf life, and has the potential to be developed into an environmentally friendly biological agent.

The application discloses a single-head up-and-down reciprocating semi-rotation pesticide spraying device and belongs to the technical field of agricultural machinery, which comprises a pesticide box, an arc hollow frame body, a transmission mechanism, a displacement mechanism and a pesticide spraying mechanism. The rotation of a roller drives the rotation of the transmission mechanism, and then drives the rotation of the displacement mechanism. The pesticide spraying mechanism is connected with the displacement mechanism. When the displacement mechanism moves, the pesticide spraying mechanism is driven to move. In the movement process of the pesticide spraying mechanism, with the change of the movement track, when the pesticide spraying mechanism sprays pesticides from top to bottom, the upper surface of grape leaves is sprayed first. When the pesticide spraying mechanism sprays pesticides from bottom to top, the lower surface of grape leaves is sprayed. The full-range uniform spraying of grape leaves is realized. Meanwhile, when the grape leaves are sprayed from the side, the problem that the lower grape leaves close to grape stems cannot be sprayed is reasonably solved.

The insect pest prevention device comprises a medicine storage tank, an elastic supporting piece and a water pump, the bottom of the medicine storage tank is provided with the elastic supporting piece, the water inlet end of the water pump is communicated with a culture pond through a first pipeline, and the water outlet end of the water pump is communicated with the culture pond through a second pipeline. The second pipeline is provided with a necking part of which the diameter is reduced, and the necking part is communicated with the medicine storage tank through a third pipeline. According to the insect pest prevention device for sea grape culture, liquid medicine is mixed with water outside a culture pond and then injected into the culture pond, and the situation that the concentration of the liquid medicine in the water is too high and physical disturbance is caused is avoided.

The invention discloses a vineyard fruit fly trapping agent and application thereof. The trapping agent is prepared from diseased grape fruits which are damaged by diseases and lose commodity, ethyl alcohol, acetic acid, water and dishwashing liquid according to the proportion; a waste plastic bottle is refitted to be manufactured into the fruit fly trap; the trapping agent is poured into a trap, and the trap is hung in a vineyard to trap fruit flies. Sick fruits in a vineyard are reused, the preparation process is simple, the cost is low, application of chemical agents in the grape mature period is avoided, and the fruit fly trapping agent is environmentally friendly and has a good fruit fly trapping effect.

The utility model discloses a sunshine rose grape seedling cultivation protection device, which belongs to the technical field of agricultural planting, and comprises a base, the lower surface of the base is fixedly connected with a plurality of positioning columns, the positioning columns are arranged at the radial position of the base, and the lower surface of the base is fixedly connected with the sunshine rose grape seedling cultivation protection device. A boss is integrally formed on the inner edge of the base, a vertical rod is fixedly connected to the upper surface of the boss, a plurality of limiting grooves are annularly formed in the outer wall of the vertical rod at equal intervals in the longitudinal position of the vertical rod, binding belts are installed at the limiting grooves, and a transverse rod is fixedly connected to the side wall of the top end of the vertical rod. According to the sunshine rose grape stand, it can be guaranteed that sunshine rose grape seedlings grow vertically and upwards, leaves of the sunshine rose grape seedlings can be better irradiated by sunshine, and therefore the efficiency of photosynthesis is improved, meanwhile, pests are trapped and killed, and the seedlings are fully protected.

The application discloses a mulberry leaf astringency removing treatment process and super-micro powder thereof. The process mainly uses a fermented coix as an astringency removing agent to remove the astringency of frost mulberry leaves in combination with three steaming and hot air drying methods. The application solves the problems of astringency and green smell of the mulberry leaves by using a relatively simple process, retains the unique taste and fragrance of the mulberry leaves, and adds unique alcohol and ester fragrance, so that the taste and smell of the mulberry leaves are rich in layers. The prepared astringency removed mulberry leaf super-micro powder has high retention rate of main nutritional components, has obvious inhibition effect on alpha-glucosidase and pancreatic lipase, and can be used as a food base to develop health food for reducing blood sugar and blood fat.

The invention relates to the technical field of biology, in particular to a vitis davidii susceptible gene VdTLP19 as well as an encoding protein and application of the vitis davidii susceptible gene VdTLP19. The vitis davidii susceptible gene VdTLP19 is located on a chromosome 2 of vitis davidii and is distributed in a region 3655997-3656642, and the nucleotide sequence of the vitis davidii susceptible gene VdTLP19 is shown as SEQ ID No.1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID No.2. And after the vitis davidii susceptible gene VdTLP19 is silenced, the resistance of grape fruits to pathogenic bacteria is enhanced. After the VdTLP19 gene provided by the invention is silenced, the resistance of grapes to anthracnose can be improved, and a theoretical basis is provided for grape anthracnose resistance breeding.

The invention discloses a composition for treating acute upper respiratory infection and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine compositions. The composition disclosed by the invention is prepared from the following raw materials in parts by weight: 50 to 500 parts of water lily flower, 50 to 200 parts of endive, 50 to 200 parts of cerasus humilis, 50 to 300 parts of hollyhock flower, 50 to 300 parts of hyssopus officinalis, 50 to 300 parts of artemisia rupestris and 50 to 200 parts of tripterygium hypoglaucum. The composition is granules. The granules are successfully prepared by adopting a whole-course low-temperature stepped extraction and refining process. According to the process, the retention rate of heat-sensitive effective components is remarkably increased, the medicine effect basis is ensured, meanwhile, by reducing impurity dissolution, the product has the excellent stability of being fast in dissolution, not prone to moisture absorption and uniform in quality, and reliable conversion from a traditional empirical prescription to a high-quality modern preparation capable of being industrially produced is achieved.

The invention belongs to the technical field of pesticide chemistry, and relates to a quinoline derivative containing a piperazine structure and a preparation method and application thereof. The invention provides a quinoline derivative containing a piperazine structure, and the quinoline derivative has broad-spectrum inhibitory activity on phytopathogens such as plant pathogenic fungi, especially has excellent inhibitory activity on valsa mali, sclerotinia sclerotiorum, botrytis cinerea, gaeumannomyces graminis and magnaporthe oryzae. The bactericide is expected to be developed into a novel green bactericide which is efficient, safe, economical and environment-friendly.

The invention belongs to the technical field of fruit tree cultivation and management, and particularly relates to a pre-stepped saline-alkali domestication saline-alkali soil grape seedling field planting method which comprises the following steps: S1, preparing saline-alkali simulation solutions with three concentration gradients of 2g / L, 4g / L and 6g / L according to soil saline-alkali types; s2, before field planting, carrying out stepped saline-alkaline domestication on grape seedlings growing in the culture medium in a stress-recovery alternating mode, and adding 10-50 [mu] M of ABA into 6g / L saline-alkaline simulation liquid for common treatment; s3, after domestication is completed, the domesticated nursery stocks are subjected to field planting on ridges, drip irrigation and film mulching for soil moisture conservation, and management is carried out according to conventional cultivation standards. Through the technical combination of saline-alkali simulated liquid gradient intermittent domestication, exogenous hormone regulation and control combination and the like, the field planting method remarkably improves the field planting survival rate of the grape seedlings in the saline-alkali soil, and is particularly suitable for high-survival-rate field planting and early-stage standardized management of grapes under the medium-high saline-alkali soil condition.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The application discloses a composition with soothing and itching-relieving effects and a preparation method and application thereof, and belongs to the cosmetic field; the composition comprises the following components: a composite plant extract, a polyhydric alcohol and water; the composite plant extract is compounded by a polygonatum sibiricum redoute fermentation liquor, a paeonia extract and a long-stemmed grape caulerpa lentillifera extract; and the polygonatum sibiricum redoute fermentation liquor is obtained by composite fermentation of lactobacillus pentosus and bacillus subtilis. The soothing and itching-relieving composition provided by the application has excellent soothing effects, and the soothing and itching-relieving composition provided by the application has excellent in-vitro hyaluronidase inhibition effects, and the in-vitro hyaluronidase inhibition rate reaches more than 60%.

The application discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of bacteriostatic drugs, and has the technical scheme as follows: the active ingredient of the preparation is composed of isoeugenol and bacillus velezensis fermentation liquor.The half bacteriostatic concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the bacteriostatic capacity is higher than that of the existing technology of bacto in the same concentration.Based on the influence on the membrane permeability of filamentous fungi, the application can synergistically inhibit the pathogenic activity of postharvest fungi of fruits, and is expected to be used for preparing a novel preparation for preventing and treating high-sugar fruits after harvesting.The application is simple to use, environment-friendly, can be directly smeared on the surface of fruits in the planting stage, can not be directly contacted with the fruits in the storage period, and can provide the bacteriostatic capacity for the storage period by using the fumigation mode.The application provides a new choice for the biological prevention of fruits, and has good economic benefits and social benefits.

This novel nonpathogenic bacterial strain exhibiting a controlling effect on crown gall disease in plants and tomato canker disease is the Allorhizobium vitis A3 strain (accession number: NITE BP-041154).

This invention discloses the tea plant miRNA miR408-p5 and its application and method in improving plant disease resistance. By constructing a silencing expression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, exhibiting strong fungal inhibitory activity against *Botrytis cinerea*. Conversely, by constructing an overexpression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, showing significantly weakened resistance to *Botrytis cinerea*. Therefore, silencing miR408-p5 expression can improve plant disease resistance, and introducing the miR408-p5 silencing expression vector into plants such as tobacco, tea, or vegetables to enhance plant disease resistance has broad market application prospects.

The invention relates to the field of plant biotechnology and molecular biology, in particular to application of a VvECT10 gene in regulation and control of drought resistance of grapes. A strain with overexpressed VvECT10 gene is slow in leaf water loss and light in withering degree under a drought condition, the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) is remarkably reduced, and the activity of peroxidase (POD) and catalase (CAT) is remarkably improved, which indicates that the overexpression of the VvECT10 gene can enhance the drought resistance of the grape plant and relieve oxidative damage.

The invention concerns a method for prophylaxis of infections by fungi, particularly by oomycetes, and of bacterial infections in crop and ornamental plants. Areas of application are vine, fruit, vegetable and ornamental plant growing. The method of the invention is characterized in that an aqueous solution of a protease, alone or in combination with β-glucanases and / or chitinases, is prepared, stabilizers, stickers and wetters are added, and the additized solution is applied by conventional techniques to the plants a number of times within the vegetation period, preferably ahead of weather-related phases of high infection threat.

The invention discloses a mixed fermentation brewing method of Xuangxiang kiwi fruit and sunshine rose grape composite fruit wine, and belongs to the technical field of fruit wine brewing. According to the method, Xuangxiang kiwi fruits and sunshine rose grapes are used as raw materials and are compounded according to the mass ratio of 2: 1, SO2 and pectin lyase are added, independently-bred pichia kluyveri HN22 and commercial saccharomyces cerevisiae RV171 are adopted for mixed fermentation, low-temperature natural clarification, cold stabilization, multi-stage membrane filtration and other treatment are performed, and the high-purity kiwi fruits and sunshine rose grapes are obtained. The composite fruit wine is rich in fragrance, moderate in acidity and low in methanol content. According to the preparation method, raw material ratio optimization is combined with a mixed fermentation process, so that the problems that the traditional kiwi fruit wine is boring in aroma and sour and astringent in taste and methanol is easy to exceed the standard are solved, the sensory quality of a product is remarkably improved, and the preparation method has a good industrialization prospect.

The utility model discloses a cold shed grape cold -resistant cultivation support relates to grape cultivation field, including bottom plate, the bottom plate is detachably installed with connecting plate through bolt connecting piece, one side of connecting plate is rotatably connected with inclined bracing piece, the end rotatably connected with crossbeam of inclined bracing piece, the middle rotatably connected with ground support rod of inclined bracing piece. Through the inclined bracing piece is inclined, and the ground support rod is rotated and is inclined to make the soil breaking cone to be inserted into the ground, the inclined bracing piece, the ground support rod and the ground are triangle stable support between this time, again overturn crossbeam to with the ground horizontal state, and are fixed through the plug -in component, finally can when the grapevine is fixed on the inclined bracing piece through the ribbon when the grapevine on the inclined bracing piece grows the fruit, the grapevine forms certain angle with the ground, and the fruit is naturally drooping under the action of gravity, forms the three -dimensional cultivation mode of staggered, avoids the mutual superposition shading, has improved the yield of grape.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of ningnanmycin salt in inhibiting phytopathogen. Derivatives of ningnanmycin salt are developed on the basis of ningnanmycin, and the ningnanmycin salt is applied to resisting ascomycophyta and oomyceta phytopathogens including valsa mali, pyricularia grisea, phytophthora capsici, physalospora piricola, botrytis cinerea, fusarium graminearum and the like. Experiments show that the ningnanmycin salt significantly improves the inhibitory activity of ningnanmycin on phytopathogens, more effectively inhibits various phytopathogens, realizes a wider-spectrum bactericidal effect, and has a good application prospect.

The patent discloses a method for quickly establishing a Cabernet Sauvignon grape pericarp callus regeneration system, and belongs to the technical field of cell engineering. The method takes the pericarp of Cabernet Sauvignon grape as an explant, realizes efficient induction and rapid regeneration of callus through three key steps of explant disinfection treatment, primary callus induction culture, callus proliferation and subculture. The method optimizes the culture medium formula, adds specific concentrations of 6-BA, KT, IAA, NAA and 2,4-D in the induction medium, and significantly improves the induction rate and growth rate of callus. Experimental results show that the stable callus regeneration system can be obtained in 40-50 days by using the method, the callus induction rate can reach more than 90%, and the survival rate of regenerated plants is high. The patent has the advantages of simple operation, good repeatability and short cycle, and provides an effective technical means for genetic improvement, gene function research and rapid propagation of wine grapes, and has important theoretical value and application prospect.

This invention provides a high-efficiency seedless grape cultivation technology. Through systematic innovation in seed pretreatment, compound growth regulator formulation, substrate optimization, and precise cultivation management, it overcomes traditional technological bottlenecks. Seed pretreatment employs a quicklime-distilled water activation method combined with wood ash-milk fermentation and ultrasonic-assisted treatment, significantly improving embryo activity and germination rate. The growth regulator is a golden ratio compound of gibberellin and chlorpyrifos, applied to the flower spikes twice, with sodium tungstate added to prolong efficacy, achieving a seedless rate >95% and a 20%-30% increase in berry weight. The substrate uses a multi-stage formulation system, adjusted to pH 5.0-5.5 after low-temperature sterilization, and incorporates 5% biochar to enhance water retention and pollution resistance. Innovative cultivation management includes optimized H-shaped trellis system combined with rain-sheltered cultivation and precise water and fertilizer management, resulting in a stable yield of over 1500 kg / mu, extended shelf life to 15 days, and a cultivation cycle shortened to 2 years before fruiting. This technology comprehensively improves fruit quality and planting efficiency, demonstrating significant application value.