280 results about "Fruit set" patented technology

The present invention relates to the fields of biotechnology and plant breeding, specifically providing a method for fixing heterosis in rice using the DMP gene. To address the low fruit set rate and complex operation issues of existing apomictic reproduction systems, the present invention verifies for the first time the haploid-inducing function of the rice genes OsDMP8 and OsDMP13. Furthermore, the CRISPR / Cas9 technology is used to simultaneously knock out the OsPAIR1, OsREC8, OsOSD1, and OsDMP genes, constructing a "MiMe-OsDMP" four-gene editing system. This system provides new ideas for constructing apomictic reproduction systems in rice and offers a new solution for fixing heterosis in rice apomictic reproduction. This system can significantly improve the efficiency and quality of agricultural production, and has significant economic value and broad application prospects.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

The invention relates to the technical field of grape planting, in particular to a grape nutrition regulation-based early-maturing high-quality light and simplified production method, which comprises the following steps of: regulating nutrition distribution in a germination period, and taking moisture-preserving and rain-sheltering measures on the ground and branches; regulating and controlling nutrient distribution in the flowering period to control branch growth, and ensuring the fruit setting rate based on pinching measures; management is conducted in the fruit expanding period, on the basis of vigorous growth control combined with high photosynthetic management, the photosynthetic efficiency of leaves is ensured, soil and leaf fertilization are adjusted, and it is ensured that nutrient supply meets the fruit expanding requirement; nutrition in the color changing period is regulated and controlled, fruit color changing is promoted based on trunk girdling, specific fertilizer is sprayed to leaf surfaces, and it is ensured that the fruit ripening and color changing process is accelerated; management is conducted in the acid-removing and sugar-increasing period, and the fruit acidity is gradually reduced based on regulation and control of nutrient distribution and fertilization measures. According to the method, the efficiency of early-maturing, high-quality and light-weight production of grapes is improved.

The invention discloses a microbial agent for promoting cucumber growth and a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. The microbial agent is prepared from the following components: achromobacter denitrificans, bradyrhizobium angularis and Vilnelandan nitrogen-fixing bacteria in a volume ratio of 2 to 1 to 1. Bacteria with a growth promoting effect are reasonably combined for use, so that the growth promoting effect of the mixed flora on cucumbers is better than that of a single strain, and the adaptability of the synergistic effect of the strains in the actual soil rhizosphere complex environment is higher. By determining the number of strains and mixing according to a specific proportion, germination of cucumber seeds is stably and effectively promoted, root elongation is promoted, the flowering and fruit setting rate is increased, and meanwhile the plant height, plant biomass and chlorophyll content of cucumbers in the seedling stage are increased. Besides, the microbial agent disclosed by the invention is beneficial to improving the growth quality and yield of plants and reducing environmental pollution, has wide application potential in the fields of agriculture and ecology, and is worthy of popularization.

The invention discloses a device for fruit trees to resist freeze injury. The device comprises a combustion mechanism, a heat passing pipeline and a lifting adjusting assembly. The combustion mechanism releases heat and generates smoke in a fuel combustion mode. The heat passing pipeline is installed at a smoke outlet of the combustion mechanism, a smoke outlet is formed in the tail end of the heat passing pipeline, and the heat passing pipeline is used for conveying heat released by fuel combustion and smoke generated by fuel combustion to plant and row spaces between fruit trees in an orchard; the lifting adjusting assembly is used for detecting the temperature in the orchard and adjusting the position of the smoke outlet. According to the device for the fruit tree to resist the freezing injury, the freezing injury influence of low-temperature weather on branches, flowers and buds of the fruit tree can be remarkably reduced, a more favorable environment is created for growth of the fruit tree, the fruit setting rate of the fruit tree is effectively increased, and the yield and income of an orchard are guaranteed.

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The present invention discloses a sorting system and device for honey peaches based on visual detection and deep neural network. The method includes: acquiring an image of a fleshy fruit to be sorted; performing image segmentation processing on the image of the fleshy fruit to obtain a first feature; processing the first feature through an appearance defect detection model to obtain an appearance detection result; and performing elimination processing on the fleshy fruit based on the appearance detection result to obtain a first fruit set; determining the quality category of the fruit according to the type and shape area of each fruit in the first fruit set, and sorting the fruit according to the quality category. The embodiments of the present application are beneficial to improving the detection efficiency of the appearance defect detection model and the sorting accuracy. This method can be widely applied to the technical field of sorting systems.

The present application provides a method and device for thinning flowers and fruits based on image recognition. The method obtains a first image of a fruit tree to be processed captured by an image acquisition device. Based on the first image, the fruit tree type of the fruit tree to be processed is determined. According to the fruit tree type, a flower and fruit thinning model that matches the fruit tree type is determined. Based on the flower and fruit thinning model, a plurality of target information to be processed corresponding to the fruit tree to be processed and the attribute value of each target to be processed are determined. Among them, the target to be processed includes at least a flower bud image and a young fruit image. The attribute value represents the fruit setting rate of the target to be processed after processing. The first image, the second image of each target to be processed and the attribute value of each target to be processed are sent to a preset expert planting model to determine the processing risk value of the target to be processed. Based on the attribute value of each target to be processed and the corresponding processing risk value, the processing target is determined so that the flower and fruit thinning management terminal corresponding to the image acquisition device thins the flowers and fruits of the fruit tree to be processed according to the processing target.

The invention provides a system (1000) for cultivating a pepper plant (10), wherein the system (1000) comprises a light generating device (100) and a control system (300), wherein the light generating device (100) is configured to provide device light (101) to the pepper plant (10), wherein the device light (101) comprises one or more of red light and far red light, wherein the red light comprises one or more wavelengths in the range of 600-700 nm, and wherein the far red light comprises one or more wavelengths in the range of 700-800 nm wherein the system (1000) has an operational mode wherein: the control system (300) is configured to acquire an input signal, wherein the input signal relates to a plant load-related parameter of the pepper plant (10), wherein the plant load-related parameter is selected from the group comprising a plant load, a flowering rate, a flower abortion rate, a fruit set rate, and a fruit abortion rate; the control system (300) is configured to determine a plant load based on the input signal, and wherein one or more of the following applies: (i) the control system (300) is configured to compare the plant load to a lower plant load threshold, and to select a target R:FR ratio of red light to far red light from the range of >15 if the plant load is lower than the lower plant load threshold; and (ii) the control system (300) is configured to compare the plant load to a higher plant load threshold, and to select the target R:FR ratio from the range of <15 if the plant load is higher than the higher plant load threshold; the control system (300) is configured to expose the pepper plant (10) to the target R:FR ratio by controlling the light generating device (100).

The invention relates to a watermelon sweetening cultivation method. The watermelon sweetening cultivation method comprises the following steps: S1, applying a thoroughly decomposed organic fertilizer and a potassium-magnesium compound fertilizer which are uniformly mixed into soil as a base fertilizer before planting; s2, atomizing and spraying sweeteners to leaf surfaces and flowers of the watermelons in flowering and fruit setting periods; s3, a sweetener is sprayed to leaf surfaces from a watermelon fruit swelling period to a watermelon fruit maturation period, a nursing blender is sprayed to the surfaces of the watermelon fruits, and a nursing blender is sprayed to the surfaces of the watermelon fruits; the nursing blender is prepared from the following raw materials in parts by weight: 5 to 16 parts of beewax, 2 to 7 parts of porous calcium particles, 1 to 3 parts of seaweed extract, 0.5 to 3 parts of fructo-oligosaccharide, 0.5 to 1.5 parts of glycerol, 0.8 to 1.5 parts of span-80 and 70 to 90 parts of distilled water. The problem of how to reduce the poor sweetness from the center of the watermelon to the surface layer of the watermelon can be solved, the overall sweetness of the watermelon can be improved, and the watermelon explosion rate can be reduced.

The invention relates to the technical field of plant planting, in particular to a method for promoting flowering and fruit setting of amomum tsao-ko and application thereof.In the vigorous vegetative growth period, a nutrient solution composed of ammonium sulfate, NAA and GA3 with the proper concentration is adopted, the vigorous vegetative growth period can be effectively prolonged, and therefore flower bud differentiation is inhibited; a nutrient solution B composed of monopotassium phosphate, ABA and BR is sprayed in April, vegetative growth can be effectively broken through to be converted into reproductive growth, flower buds are promoted to be rapidly differentiated and formed when a rainy season comes, the flowering rate can be increased by 28-33%, the yield of the amomum tsao-ko medicinal material can be greatly increased, bumblebees are quantitatively put in the early stage of May, and the pollination rate can be greatly increased; in the blooming period, the nutrient D is spread around the cluster buds, meanwhile, the nutrient solution E is sprayed in a matched mode, and the fruit setting rate can be increased by 8.9%-9.6% compared with the fruit setting rate.

This invention provides a method for storing mango seeds, belonging to the field of seed preservation technology. The method involves cutting off mango fruits with embryos, along with their pedicels and branches, 77-84 days after fruit set. A mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed on the fruit, pedicels, and branches. After drying, styrene-butadiene latex is applied to the pedicels and branches. Once the latex dries, a mixture of guanidine-copper acetate and benzoyl-pyraclostrobin is sprayed on the fruit, pedicels, and branches again. After drying, the mango fruits with petioles and branches are placed in a sealed box and stored at 10-14°C. The mango variety used is the Golden Phoenix mango. This invention increases the storage period of Golden Phoenix mango seeds from 1.5 months to 5 months, which is of great significance for the genomics and cell biology research of Golden Phoenix mangoes.

The disclosure provides a system for cultivating a pepper plant, wherein the system comprises a light generating device and a control system, wherein the light generating device is configured to provide device light to the pepper plant, wherein the device light comprises one or more of red light and far red light, wherein the red light comprises one or more wavelengths in the range of 600-700 nm, and wherein the far red light comprises one or more wavelengths in the range of 700-800 nm.

The invention relates to the technical field of gene engineering, in particular to application of a knockout SlABCB13 gene in increasing the soluble sugar content of tomato fruits. The function of the SlABCB13 gene is deleted by mediating gene editing, so that the content of soluble sugar (cane sugar, fructose and glucose) and the content of soluble solids in tomato fruits are remarkably increased, and the SlABCB13 participates in sugar transport of the tomato fruits. Meanwhile, the mutant does not influence related yield indexes such as plant height, stem diameter, fruit size, fruit setting number and single-plant fruit yield, and a new thought is provided for the problem that yield and quality are difficult to consider in existing tomato breeding.

The invention discloses a method for carrying out water-saving irrigation according to temperature difference inside and outside a rice canopy, which is characterized in that the temperature difference inside and outside the rice canopy is used as a diagnostic index of irrigation, and an irrigation water layer in a field is determined according to growth periods and soil types. Compared with a conventional irrigation method, the method for carrying out water-saving irrigation according to the difference value of the internal temperature and the external temperature of the rice canopy has the advantages that the number of grains per ear, the maturing rate and the thousand seed weight are remarkably increased, so that the yield is remarkably increased, and the amplification is 9.65%-14.46%. Compared with a conventional irrigation method, the method for carrying out water-saving irrigation according to the difference value of the internal temperature and the external temperature of the rice canopy can reduce the irrigation water consumption by 24.33%-26.38%, and the utilization efficiency of the irrigation water can be improved by 46.71%-53.23%.

The invention discloses a planting method for delaying flowering and fruit setting of citrus, and belongs to the technical field of citrus planting. The planting method comprises the following steps that S1, autumn shoots are promoted to sprout and mature, before the autumn shoots sprout, existing branches are trimmed, nitrogenous fertilizer, phosphate fertilizer and potash fertilizer are applied, and 0.2-0.4% monopotassium phosphate and 200-300 ml / L paclobutrazol are sprayed after new shoots are self-trimmed; s2, spring shoot germination inhibition: before spring shoot germination, rain sheltering and water control are carried out, and soil drought is kept; s3, sprouting of early summer shoots is promoted, before sprouting of the early summer shoots, existing branches are trimmed, and nitrogenous fertilizer, phosphate fertilizer and potash fertilizer are applied; s4, fruit retention: spraying a fruit retention agent during the end-flowering period and after the first physiological fruit drop period. The method can delay the maturing and marketing time of citrus, and prolongs the fresh fruit supply period by about 90 days compared with a conventional planting mode.

This invention belongs to the field of biological agents and plant breeding technology, specifically relating to the application of *Trichoderma tumefaciens* T-51 in promoting flowering, fruit setting, and vegetative growth in plants. The *Trichoderma tumefaciens* T-51 described in this invention has the preservation number CCTCCNO: M2015729. Applying a suspension containing *Trichoderma tumefaciens* T-51 to the roots of plant seedlings can advance the flowering time and increase the number of flowers; it can also improve the number and rate of fruit set; and it can promote vegetative growth, especially increasing plant height, number of stem nodes, and number of leaves, thereby accelerating the plant breeding process and shortening the breeding cycle. This invention has very high application value in plant breeding.

The invention belongs to the technical field of agriculture, and particularly relates to a plant growth regulator containing 14-hydroxyl brassinosterol and indolebutyric acid, the plant growth regulator comprises an active component A 14-hydroxyl brassinosterol, an active component B indolebutyric acid, and a biological enzyme preparation. The plant growth regulator provided by the invention can play an important role in the aspects of flowering, fruit setting and yield of plants, fruit quality, disease resistance and the like, improves the effects of 14-hydroxybrassinosteroid and indolebutyric acid, reduces the risk of adverse reaction or phytotoxicity, and has the advantages of wide application prospect, high market demand and huge commercial value.

The invention relates to the technical field of agricultural fertilization, in particular to a poly-carbon fertilizer application device for improving the fruit setting rate and yield of macadamia ternifolia, which comprises a tumbrel main body, a fertilization assembly, a stirring and feeding assembly and a fertilizer quantifying assembly, the fertilization assembly is mounted on the outer wall of one side of the tumbrel main body, and the fertilization assembly is mounted on the outer wall of the other side of the tumbrel main body; a stirring and feeding assembly is mounted on the tumbril main body, and fertilizer quantifying assemblies are mounted on the two sides of the tumbril main body; the fertilizing assembly comprises a lifting groove, a first spring, a sliding plate, a plow harrow device, a first motor and a first rotating shaft; according to the nut fertilizing device, the fertilizing assembly is adopted, soil of root systems of nuts can be turned, so that fertilizing treatment can be conveniently carried out on the turned soil, fertilizer can be sprayed to the leaf surfaces of vegetation, the fertilizing process is simplified, and the fertilizing effect is improved; and the stirring and feeding assembly is adopted, so that the fertilizer can be fed and stirred, manual auxiliary stirring of the fertilizer is not needed, the stirring burden is reduced, and the stirring effect is improved.

The invention relates to a macadamia nut flower and fruit retention synergistic method which comprises the following steps: in the early stage of flower bud differentiation, annularly applying slow-release granules to the root of a macadamia nut tree, and spraying fructan emulsion to the crown at the same time; in the early flowering period, a water dispersible sustained release preparation containing microencapsulated compound sodium nitrophenolate is sprayed to tree crowns of macadamia nuts, sodium alginate embedded borax sustained release microsphere suspension liquid is sprayed to leaf surfaces, and a hydrogel complexing agent containing sustained release monopotassium phosphate and amino acid is sprayed to the leaf surfaces; in the full-bloom stage, a composite slow-release emulsion containing liposome-embedded compound sodium nitrophenolate and brassinolide is sprayed to tree crowns of the macadamia nuts, slow-release gel microsphere suspension liquid of borax and amino acid is sprayed to leaf surfaces, and slow-release nano-emulsion containing beta-cyclodextrin-embedded bee wax volatile substances is sprayed to the tree crowns; in the initial fruit setting stage, sulfur-coated slow-release compound fertilizer is applied to the roots of macadamia nut trees, and ionic cross-linked calcium alginate microsphere embedded amino acid slow-release foliar fertilizer is sprayed to the leaf surfaces of the macadamia nut trees.

The invention relates to the technical field of fruit tree cultivation management, and particularly discloses an efficient flower thinning agent for peach trees and application thereof, and the flower thinning agent comprises the following main effective components: T6P with the concentration of 250-750 mg / L and ethephon with the concentration of 50-100 mg / L. According to peach trees treated by the flower thinning agent, the fruit setting rate is controlled between 20% and 25%, the weight of a single fruit reaches 190 g or above, the SSC content exceeds 12%, the fruit hardness is kept between 6.5 kg / cm < 3 > and 7.0 kg / cm < 3 >, and the fruit quality is remarkably improved. The formula is simple in components, low in cost and suitable for large-area popularization; the influence of environmental factors is small, and the stable flower thinning effect can be kept under different weather conditions.

The invention belongs to the technical field of planting, and discloses a hormone and leaf surface boron fertilizer spraying method for improving the fruit setting rate of macadamia ternifolia, and the method comprises the following steps: spraying 0.02% boron fertilizer in the flower bud period of macadamia ternifolia; 2 mg / L 6-BA is sprayed in the full-bloom stage of the macadamia nuts; 0.02% of boron fertilizer and 0.2 ml / L of brassinolide are sprayed from the flowering period of the macadamia nuts to the initial fruit setting period. The 0.02% boron fertilizer is sprayed in the flower bud period of the macadamia nuts, so that the pollen viability and the flower quantity of the macadamia nuts are increased, and the fruit setting probability and the total fruit setting quantity in the later period are increased. According to the method, 2 mg / L 6-BA is sprayed at the full-bloom stage of the macadamia nuts, so that the flower falling rate is reduced, and the fruit setting rate is increased.

The present invention discloses a method for solving the abortion of potato varieties as hybrid male parents, which includes several steps such as the cultivation and selection of rootstocks, the cultivation of scions, grafting, post-graft management, cross-pollination, and harvesting of true seeds. The present invention grafts the potato variety Jizhangshu No. 3 as the scion with Jizhangshu No. 8 as the rootstock into one entity, and then uses the grafting community of Jizhangshu No. 8 and Jizhangshu No. 3 as the male parent to cross with Jizhangshu No. 8 as the female parent to achieve the purpose of normal fruit set after pollination, and solves the problem of flower sterility of potato Jizhangshu No. 3 as a hybrid male parent.

The invention provides a method for reducing the dead fruit rate of macadamia nuts and preventing pericarp yellowing, which comprises the following steps: pruning fruit trees in the early stage of flower bud differentiation, spraying a composite insecticide in the early stage of flowering, and keeping the soil moisture content to be 60% or above. Spraying fruit retention liquid in a flower thinning and fruit retention period, using a root conditioning fertilizer and an insecticide in a flower falling and fruit setting period, spraying a leaf fertilizer and an insecticide in a fruit expanding period, and applying potassium sulfate in a final shell hardening and grease conversion period. By adding the anti-stiff fruit root slow-release fertilizer into the root conditioning fertilizer, it is guaranteed that fruit trees can continuously absorb mineral elements in high-temperature weather to reduce the stiff fruit rate, foliar fertilizer is sprayed to improve cell antioxidant enzyme activity and form a protective film, and the yellowing probability is reduced, so that the passing rate and peel yellowing of the fruit trees in the growth cycle are reduced, and the fruit quality is improved. The economic benefits of the macadamia nuts are greatly improved.

The application discloses a method for delaying the flowering period of Korla fragrant pear, comprising the following steps: checking the weather forecast at the flower bud germination period of the Korla fragrant pear, the weather forecast showing that the late spring cold will come in the predicted full flowering period of the Korla fragrant pear, and spraying the mixed agent of Flu and TIBA on the whole plant of the Korla fragrant pear at the flower bud germination period of the Korla fragrant pear to induce the Korla fragrant pear to delay flowering, wherein the concentration of Flu in the mixed agent of Flu and TIBA is 100 mg / L, and the concentration of TIBA is 100 mg / L. The application can delay the flowering period of the Korla fragrant pear, reduce the influence of low temperature on the growth and development of the flower organs of the Korla fragrant pear and pollination and fertilization, overcome the climate condition, and guarantee the productivity as much as possible, thereby improving the fruit setting rate, yield and quality of the Korla fragrant pear, providing technical guidance for preventing the Korla fragrant pear from the harm of the late spring cold and high quality and high yield, and having a wide application prospect.

The present invention relates to the field of microbial technology, and in particular to a cold-resistant Bacillus IVF-WKB47 and its use. The present invention provides a cold-resistant Bacillus ( Peribacillus frigoritolerans ) IVF-WKB47, with a deposit number of CGMCC No.28315. The Bacillus IVF-WKB47 or its microbial preparation has the effect of promoting plant growth and increasing crop yield, including increasing the number of fruits set in the plant, increasing the weight of a single fruit in the plant, etc.

The invention discloses a rapid propagation method for a hybrid orchid seedling of a cymbidium orchid and a cymbidium orchid, including hybrid breeding, seed treatment, seed germination, cluster bud induction and rooting culture procedures, selecting cymbidium orchid and a cymbidium orchid for hybrid breeding according to a conventional method, using the obtained hybrid seeds as explants, cleaning and disinfecting the explants, and then sequentially inoculating them in a primary culture medium, an induction culture medium and a rooting culture medium, and culturing them in a suitable environment, and finally obtaining hybrid orchid seedlings of a cymbidium orchid and a cymbidium orchid. The invention adopts pollen pollination at an optimal time stage to improve the fruit setting rate, adopts a high-efficiency and low-toxicity disinfectant to reduce the contamination rate of the explant, and minimizes the effect of the disinfectant on the growth of the explant, selects a suitable culture medium for different growth stages of the hybrid orchid of a cymbidium orchid and a cymbidium orchid, shortens the production and seedling raising cycle, preserves the excellent characteristics of the individual plants to the greatest extent, and promotes the large-scale development of hybrid orchid seedling breeding.

The invention belongs to the technical field of agricultural planting, and particularly relates to a method for increasing the fruit setting number of macadamia nuts, which comprises the following steps: separating selected macadamia nut female parent and male parent plants by using an insect-proof net before flowering so as to avoid the influence of insects; after the female parents enter a full-bloom stage, selecting sunny weather with the daytime air temperature of 20-30 DEG C and the wind speed of less than 1m / s, cleaning original pollen on styluses of the female parents by using a sterile writing brush, brushing the collected pollen of the male parents on the styluses by using a brush pen for pollination, keeping isolation for 14 days after pollination, and then removing the insect-proof net. The high-affinity pollination combination is screened by combining germplasm such as Guijin No.1, Guijin A4 and Guijin YM26 with pollen viability. The optimal pollination combination is screened by evaluating the affinity of 16 hybrid combinations of 10 germplasms of macadamia nuts. Results show that when A4 is used as a male parent and Guihot No.1 is used as a female parent, the affinity of cross pollination is the highest, the success rate of cross pollination is remarkably improved, the average fruit setting number is remarkably increased, and effective technical support is provided for high-yield cultivation of macadamia nuts.

This invention belongs to the field of vegetable cultivation technology, specifically relating to a treatment method for enhancing the low-temperature resistance of tomato anthers and its application. The treatment method for enhancing the low-temperature resistance of tomato anthers includes the following steps: spraying with methyl jasmonate during the pre-opening stage of tomato flowers in a low-temperature environment; the low-temperature environment refers to an ambient temperature below 15℃. This treatment method can effectively alleviate the decline in pollen viability, reduced pollen tube germination rate, and adverse effects on fruit phenotype caused by low temperatures, thereby significantly mitigating the decrease in tomato fruit set rate caused by low-temperature injury.

The application discloses Zanthoxylum bungeanum miR167b The application belongs to the technical field of genetic engineering, and discloses a gene in the application of regulating plant growth and development miR167b The application constructs an expression vector of the gene, and makes the gene overexpress in tomatoes, and finds that miR167b The overexpression plant is dwarfed, the shape of the fruit is changed, the size and weight of the fruit are significantly lower than those of the wild type, the fruit setting rate is high, the fruit is seedless, the number of fruit chambers is reduced, ovule abortion occurs, and no embryo sac is formed in the interior. Further analysis finds that the indole butyric acid content in the fruit of the overexpression plant is increased, which indicates that miR167b The gene can regulate auxin to induce fruit apomixis, lays a foundation for revealing the apomixis mechanism of Zanthoxylum bungeanum, and provides gene resources and basis for improving the breeding strategy of woody plants, and has important theoretical value and application prospect.