499 results about "Plant breeding" patented technology

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The present invention relates to the fields of biotechnology and plant breeding, specifically providing a method for fixing heterosis in rice using the DMP gene. To address the low fruit set rate and complex operation issues of existing apomictic reproduction systems, the present invention verifies for the first time the haploid-inducing function of the rice genes OsDMP8 and OsDMP13. Furthermore, the CRISPR / Cas9 technology is used to simultaneously knock out the OsPAIR1, OsREC8, OsOSD1, and OsDMP genes, constructing a "MiMe-OsDMP" four-gene editing system. This system provides new ideas for constructing apomictic reproduction systems in rice and offers a new solution for fixing heterosis in rice apomictic reproduction. This system can significantly improve the efficiency and quality of agricultural production, and has significant economic value and broad application prospects.

The invention discloses a SiERF109 gene for regulating drought resistance, salt tolerance and flavonoid accumulation of millet and application of the SiERF109 gene, and belongs to the technical field of plant breeding. According to the invention, a gene SiERF109 with a nucleotide sequence shown as SEQ ID NO.1 is separated and cloned from millet, and fluorescent quantitative expression analysis shows that SiERF109 participates in drought and salt stress response of millet. After the gene is over-expressed in foxtail millet, in drought and salt stress experiments, compared with a wild type strain, the survival rate and chlorophyll content of a strain over-expressed with the SiERF109 gene are remarkably increased, the flavonoid content is increased by 33.5%-39.5%, and active oxygen accumulation is remarkably reduced; it is shown that the SiERF109 gene and the protein coded by the SiERF109 gene can remarkably improve the drought resistance, the salt resistance and the flavonoid content of the plant.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

An epigenetic regulatory factor ZmULT1 related to corn grain size regulation and heat stress response and the use thereof, which solve the technical problems of how to increase the grain size and / or heat resistance and / or lodging resistance of corn. Use of a substance for reducing the content of the protein ZmULT1 or a substance for inhibiting the expression of the encoding gene of the protein ZmULT1, which use is any one of the following: P1, the use in increasing the grain size of corn; P2, the use in improving the heat resistance of corn; P3, the use in increasing the lodging resistance of corn; and P4, the use in plant breeding. The homozygous mutant strain with knockout of the ZmULT1 gene has a reduced plant height and an increased grain size, and exhibits significant heat stress resistance, this indicates that the research on the gene can further enrich the use of epigenetics in crops and is of important significance for improving the grain size, heat resistance and lodging resistance of corn.

The invention provides application of a tobacco NtMYC1 gene in promoting growth and development of tobacco glandular hair, and belongs to the technical field of plant breeding. The tobacco MYC1 transcription factor is obtained by carrying out sequence characteristic analysis, conservative structural domain identification, protein physicochemical property analysis and phylogenetic analysis on a genome of tobacco. And an RNAi technology and an overexpression tobacco transgenic line verify that the tobacco MYC1 transcription factor and the coding gene thereof have the effect of regulating and controlling the growth and development of the tobacco glandular hair, and the growth and development of the tobacco glandular hair are promoted through forward regulation and control, so that the growth and development of the tobacco glandular hair are inhibited through silent expression. Therefore, the tobacco MYC1 transcription factor and the coding gene thereof provided by the invention provide a new means for tobacco breeding.

The invention discloses a polypeptide and application thereof in regulation and control of plant development, and relates to the technical field of biology. The invention specifically discloses any polypeptide from A1) to A3) as shown in the following: A1) a polypeptide with an amino acid sequence as shown in SEQ ID NO: 1; a2) a polypeptide which is obtained by substitution and / or deletion and / or addition of more than one amino acid residue on the amino acid sequence as shown in SEQ ID NO: 1 and has the function of regulating plant growth and development; and A3) a fused polypeptide obtained by connecting a tag to the N end and / or C end of A1) or A2). The polypeptide can effectively regulate and control root system growth and root regeneration of plants, can be well applied to improvement of plant varieties, and has important practical value for plant breeding work.

The invention relates to the field of bioinformatics, in particular to a genome selection method, device and equipment, a medium and a computer program product. Integrating a plurality of machine learning models, including 13 algorithms such as a support vector machine (SVM), linear regression, Ridge regression, Lasso regression and the like; by combining automatic hyper-parameter optimization, parallel computing and efficient data preprocessing technologies, the breeding prediction precision and the computing efficiency are remarkably improved. The device comprises an IASML software module and an IASML cloud platform module, wherein software supports command line operation and is suitable for large-scale data calculation; the cloud platform provides a graphical interface and supports online data uploading, model selection, real-time monitoring and result downloading. According to the method, the problems of singleness, limited data scale, poor interactivity and the like of an existing tool model are solved, and an efficient, flexible and reproducible intelligent solution is provided for animal and plant breeding and genetic research.

The invention discloses application of protein TaTDM and related biological materials thereof, and belongs to the field of plant breeding. The amino acid sequence of the protein TaTDM is as shown in SEQ ID NO: 2 or SEQ ID NO: 4 or SEQ ID NO: 6. The TaTDM gene knockout homozygous mutant strains tatdmm1 and tatdmm2 are obtained by carrying out gene knockout on wild type wheat Fielder, and it is found that at the temperature of 25 DEG C, the total floret number of each spike of the Fielder, the tatdmm1 and the tatdmm2 and the survival rate under the water shortage treatment condition are remarkably higher than those of the wild type wheat Fielder. The protein TaTDM has an important application value in regulation and control of wheat ear type characters and stress tolerance, provides a high-quality germplasm resource, and is of great significance in cultivation of drought-enduring plant varieties and improvement of crop yield.

The invention discloses application of a rice SD10 gene in yield regulation and lodging resistance. The invention provides a protein, a substance for up-regulating or enhancing or improving the expression of a coding gene of the protein, or an application of a substance for up-regulating or enhancing or improving the activity or content of the protein in at least one of the following substances. A1) improving lodging resistance of plants; a2) increasing the plant yield; a3) cultivating lodging-resistant plants; a4) cultivating high-yield plants; a5) plant breeding; the protein comprises an amino acid residue as shown in SEQ ID No. 2; the invention proves that the SD10 gene simultaneously affects the characters such as single plant yield and stalk thickness, and plays a very important role in regulating and controlling the rice yield and lodging resistance, and the encoding nucleic acid of the protein SD10 is a high-quality gene for cultivating high-quality variety rice, and is used for cultivating high-yield and lodging-resistant rice.

In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

The invention relates to an application of a genetic transformation method of populus sibirica and a CRISPR / Cas9 gene editing method in plant breeding, and belongs to the technical field of plant molecular breeding. The method comprises the following steps: (1) soaking an explant in an infection bacterium solution; (2) spreading on a CM1 culture medium; (3) transferring the explant to a CM2 culture medium for 4-5 weeks; (4) transferring the calluses to a CM3 culture medium until adventitious buds are obtained; (5) transferring the callus with the adventitious buds to a CM4 culture medium until cluster buds are obtained; and (6) cutting off clustered seedlings, and inserting the clustered seedlings on a CM5 culture medium. According to the application of the genetic transformation and CRISPR / Cas9 gene editing method of the poplar in plant breeding, a target gene is transferred into a poplar body to obtain a positive plant, meanwhile, a poplar phytoene dehydrogenase (PDS) gene is taken as an example, a poplar mutant of which the PDS gene is knocked out is successfully obtained by applying a CRISPR / Cas9 gene editing technology, and the method is suitable for plant breeding. And a technical support is provided for application of a CRISPR / Cas9 gene editing technology in molecular breeding of the populus sibirica.

The invention belongs to the technical field of plant breeding, and particularly relates to a breeding method of high-yield multi-chamber brassica napus. Comprising the following steps: performing hybridization by taking a mustard type rape multi-chamber resource as a female parent and a cabbage type rape as a male parent to obtain an F1 generation, and performing isolated propagation to obtain F2 free seeds; selecting partial brassica napus type single plants from F2 free seeds for selfing propagation to obtain F3-generation seeds, planting the F3-generation seeds according to plant lines, performing selfing propagation, screening out single-plant selfing seeds with multi-chamber pods, continuing selfing, continuously repeating for 2-4 generations, screening out single plants closest to male parent characters, and obtaining high-yield multi-chamber brassica napus resources. The multi-chamber brassica napus resource with the multi-chamber pod rate larger than 60% is obtained through the method, the novel multi-chamber brassica napus resource with the pod length close to that of double No.11 in brassica napus parents is obtained, and the pod length of the novel multi-chamber brassica napus resource reaches 11.01 cm.

The invention belongs to the technical field of genetic engineering application and plant breeding, and particularly relates to application of a rice OsHGO gene to improvement of high-temperature tolerance of rice. The OsHGO gene in japonica rice Zhonghua 11 is knocked out through a CRISPR / Cas9 system, and a homozygous mutant plant with the OsHGO gene knocked out is obtained. The phenotypes of the wild type and mutant plants after high-temperature treatment are observed, and it is found that the wild type has more serious leaf wilt than the mutant plants. It is shown that after the OsHGO is knocked out, the influence of the high temperature on plant growth is relieved, and the OsHGO gene regulates and controls the tolerance of the rice to the high temperature. Through mutation of the gene, high-temperature-resistant rice can be cultivated.

The invention relates to the technical field of plant breeding, in particular to a method for constructing a dominant male sterile line based on CIF1 gene overexpression. The brand-new dominant male sterility gene CIF1 is found, it is verified that the gene has functions in monocotyledonous and dicotyledonous plants, and dominant male sterility resources are enriched; besides, a method capable of manually controlling the fertility recovery of the dominant sterile line is found, a reproductive breeding line for large-scale propagation is obtained, male sterile filial generations can be obtained by hybridizing the breeding line with normal plants, and a new application way is provided for large-scale application of dominant sterility.

The invention relates to the technical field of plant breeding, in particular to application of RBOHD protein or a coding gene thereof in regulating high temperature resistance of plants. The RBOHD protein comprises an amino acid sequence as shown in SEQ ID NO. 1 (sequence identifier number 1). The application further comprises cultivation of high-temperature-resistant plants and improvement of high-temperature-resistant germplasm resources of the plants. Researches find that the RBOHD protein is related to the high-temperature resistance of the plant, and the high-temperature resistance of the plant can be effectively improved by improving the expression level of the RBOHD protein. The RBOHD protein provided by the invention can be used for improving plant germplasm resources and cultivating high-temperature-resistant plants, and has important significance in the field of plant breeding.

The invention discloses an application of an LTSS9b protein and a coding gene thereof in regulating and controlling the cold resistance of rice. The invention belongs to the technical field of biology, and particularly relates to application of LTSS9b protein and a coding gene thereof in regulation and control of rice cold resistance. The protein, the substance for regulating and controlling the expression of the coding gene of the protein or the substance for regulating and controlling the activity or content of the protein can be applied as follows: A1) application in regulating and controlling the plant cold resistance and / or application in preparing a product for regulating and controlling the plant cold resistance; a2) application in plant breeding and / or application in preparation of plant breeding products; the protein is shown as a sequence 2. By inhibiting or reducing or down-regulating the activity of the protein LTSS9b in the target plant or the expression quantity of the coding gene of the protein LTSS9b, the cold resistance of the plant can be improved, and a basis is provided for cold-resistant breeding of crops.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention discloses application of novel reverse transcriptase and related fusion protein of the novel reverse transcriptase in plant-guided editing. Candidate RT protein is obtained through systematic mining from a public database, novel reverse transcriptase cl1 with activity is screened out in combination with a fluorescence report system, and a guided editing system suitable for plants is constructed. The system tests in wheat protoplast, and verifies the editing activity of cl1 to a plurality of endogenous targets. Further combining with protein structure prediction and rational design point mutation optimization, the cl1 mutant with higher editing efficiency is obtained, and the method can be applied to accurate and effective editing of plant genomes and plant breeding and improvement.

The invention discloses a gene for degrading chlorophyll as well as an encoding protein and application thereof, and belongs to the fields of molecular biology, plant genetic engineering and horticulture. Protein coded by the gene is located in chloroplast, and chlorophyll degradation can be efficiently promoted under various stress conditions. By performing different treatments on Huangkui tea trees and performing gene function verification in model plants such as nicotiana benthamiana and arabidopsis thaliana, it is found that the CsSGRs gene is subjected to induced expression by factors such as natural aging, abscisic acid, illumination intensity and low temperature, and the gene participates in a chlorophyll degradation approach, so that the functions are redundant. In addition, a bZIP family transcription factor CsABF4 is screened out, and CsSGRs gene expression can be regulated and controlled. The invention provides gene resources and theoretical basis for regulating and controlling the chlorophyll content of plants and improving related characters of plant leaf color, and has potential application value in the fields of plant breeding and gardening.

The invention relates to the technical field of plant breeding, in particular to a primer combination for detecting a regulatory element and an exogenous gene of a transgenic plant and application of the primer combination. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. 22-63. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. The application comprises: (1) transgenic plant variety germplasm supervision or transgenic plant component screening in non-transgenic varieties; (2) screening transgenic ingredients of the plant product; (3) auxiliary screening in the plant breeding process; and (4) tracing plant varieties. The invention provides a primer combination which can be applied to detection of regulatory elements and exogenous genes of transgenic plants, can realize detection of a plurality of regulatory elements and exogenous genes in a reaction system, and can meet high-throughput screening of components of the transgenic plants. The method is used for monitoring illegal planting of transgenic plants and detecting transgenic components of related plant products.

The invention relates to the technical field of biology, and discloses application of PHR2 in regulating nodulation and nitrogen fixation processes of leguminous plants under phosphorus starvation stress, and the application is any one of the following applications: P1, application in enhancing nitrogen fixation capability of the leguminous plants under the phosphorus starvation stress; p2, application in promoting formation of root nodules with nitrogen fixation capacity of leguminous plants under phosphorus starvation stress; p3, application of improving activity of nitrogenase in leguminous plant root nodules under phosphorus starvation stress; p4, application in leguminous plant breeding. The PHR2 protein and related biological materials thereof can be used for regulating and controlling the nitrogen fixation capacity of leguminous plants under phosphorus starvation stress.

The invention discloses application of ZmCOP1a and ZmCOP1b proteins and coding genes of the ZmCOP1a and ZmCOP1b proteins in regulation and control of plant yield. The invention belongs to the technical field of biology, and particularly relates to application of ZmCOP1a and ZmCOP1b proteins and coding genes thereof in regulation and control of plant yield. The proteins ZmCOP1a and ZmCOP1b or expression substances for regulating genes or substances for regulating the activity or content of the proteins can be applied to any one of the following applications: U1) application in regulating the plant yield; the invention also relates to application of the U3) in preparation of products for regulating and controlling plant yield, application of the U3) in preparation of products for regulating and controlling plant yield, application of the U4) in preparation of products for cultivating plants with changed plant yield, and application of the U5) in plant breeding.

The invention discloses a wheat heat-resistant protein TaHT-H8 as well as a coding gene and application thereof. The invention provides a protein TaHT-H8, a substance for up-regulating, enhancing or improving the expression of a coding nucleic acid of the protein TaHT-H8, or any one of the following applications of a substance for up-regulating, enhancing or improving the activity or content of the protein TaHT-H8: A1) improving the heat resistance of a plant; the heat resistance of the plant with the TaHT-H8 protein coding gene overexpressed is obviously higher than that of a wild type plant, and the TaHT-H8 protein coding gene can be used for industrial production and plant breeding.

The application discloses application of VvERF105 and VvSWEET15 promoter interaction in reducing sugar accumulation in fruits, and belongs to the technical field of biotechnology, and particularly relates to application of VvERF105 and VvSWEET15 promoter interaction in reducing sugar accumulation in fruits.The protein VvERF105 can interact with the VvSWEET15 promoter, the expression amount of the VvERF105 is regulated, the contents of grape glucose and fructose in grape fruits are negatively regulated, and the application prospect is good in the field of plant breeding.

The invention relates to the technical field of biology, in particular to application of rice LOCOs06g20960 protein to regulation and control of plant disease resistance. The invention discloses application of a substance for protecting the protein or regulating the expression substance of the coding gene of the protein or regulating the activity or content of the protein in any one of Y1-Y5: application of Y1 in regulating the disease resistance of plants; the Y2 is applied to preparation of a product for regulating and controlling plant disease resistance; the Y3 is applied to cultivation of plants with changed disease resistance; the Y4 is applied to preparation of products for cultivating plants with changed disease resistance; the invention discloses application of Y5 in plant breeding. It is found that the rice LOCOs06g20960 protein has the function of positively regulating and controlling the bacterial blight resistance of rice, the bacterial blight resistance of the rice can be remarkably improved by transferring the LOCOs06g20960 gene overexpression sequence into the rice, and the rice LOCOs06g20960 gene overexpression sequence is of great significance to cultivation of new bacterial blight resistant rice varieties.

The invention discloses molecular marker identification and application of dwarf round-grain wheat alleles. The invention belongs to the field of plant breeding, and particularly relates to molecular marker identification and application of dwarf round-grain wheat alleles. The method for identifying or assisting in identifying the wheat dwarf round grains comprises the steps that the genotype of an SNP site in a wheat genome to be detected is detected, the phenotype of the wheat dwarf round grains is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a wheat 3B chromosome, the nucleotide type of the SNP site is T or C, and the SNP site is the 185th nucleotide of a sequence 1 in a sequence table.