294 results about "Plant breeding" patented technology

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

The invention provides application of a tobacco NtMYC1 gene in promoting growth and development of tobacco glandular hair, and belongs to the technical field of plant breeding. The tobacco MYC1 transcription factor is obtained by carrying out sequence characteristic analysis, conservative structural domain identification, protein physicochemical property analysis and phylogenetic analysis on a genome of tobacco. And an RNAi technology and an overexpression tobacco transgenic line verify that the tobacco MYC1 transcription factor and the coding gene thereof have the effect of regulating and controlling the growth and development of the tobacco glandular hair, and the growth and development of the tobacco glandular hair are promoted through forward regulation and control, so that the growth and development of the tobacco glandular hair are inhibited through silent expression. Therefore, the tobacco MYC1 transcription factor and the coding gene thereof provided by the invention provide a new means for tobacco breeding.

The invention discloses application of protein TaTDM and related biological materials thereof, and belongs to the field of plant breeding. The amino acid sequence of the protein TaTDM is as shown in SEQ ID NO: 2 or SEQ ID NO: 4 or SEQ ID NO: 6. The TaTDM gene knockout homozygous mutant strains tatdmm1 and tatdmm2 are obtained by carrying out gene knockout on wild type wheat Fielder, and it is found that at the temperature of 25 DEG C, the total floret number of each spike of the Fielder, the tatdmm1 and the tatdmm2 and the survival rate under the water shortage treatment condition are remarkably higher than those of the wild type wheat Fielder. The protein TaTDM has an important application value in regulation and control of wheat ear type characters and stress tolerance, provides a high-quality germplasm resource, and is of great significance in cultivation of drought-enduring plant varieties and improvement of crop yield.

The invention belongs to the technical field of plant breeding, and particularly relates to a breeding method of high-yield multi-chamber brassica napus. Comprising the following steps: performing hybridization by taking a mustard type rape multi-chamber resource as a female parent and a cabbage type rape as a male parent to obtain an F1 generation, and performing isolated propagation to obtain F2 free seeds; selecting partial brassica napus type single plants from F2 free seeds for selfing propagation to obtain F3-generation seeds, planting the F3-generation seeds according to plant lines, performing selfing propagation, screening out single-plant selfing seeds with multi-chamber pods, continuing selfing, continuously repeating for 2-4 generations, screening out single plants closest to male parent characters, and obtaining high-yield multi-chamber brassica napus resources. The multi-chamber brassica napus resource with the multi-chamber pod rate larger than 60% is obtained through the method, the novel multi-chamber brassica napus resource with the pod length close to that of double No.11 in brassica napus parents is obtained, and the pod length of the novel multi-chamber brassica napus resource reaches 11.01 cm.

The invention belongs to the technical field of genetic engineering application and plant breeding, and particularly relates to application of a rice OsHGO gene to improvement of high-temperature tolerance of rice. The OsHGO gene in japonica rice Zhonghua 11 is knocked out through a CRISPR / Cas9 system, and a homozygous mutant plant with the OsHGO gene knocked out is obtained. The phenotypes of the wild type and mutant plants after high-temperature treatment are observed, and it is found that the wild type has more serious leaf wilt than the mutant plants. It is shown that after the OsHGO is knocked out, the influence of the high temperature on plant growth is relieved, and the OsHGO gene regulates and controls the tolerance of the rice to the high temperature. Through mutation of the gene, high-temperature-resistant rice can be cultivated.

The invention discloses application of novel reverse transcriptase and related fusion protein of the novel reverse transcriptase in plant-guided editing. Candidate RT protein is obtained through systematic mining from a public database, novel reverse transcriptase cl1 with activity is screened out in combination with a fluorescence report system, and a guided editing system suitable for plants is constructed. The system tests in wheat protoplast, and verifies the editing activity of cl1 to a plurality of endogenous targets. Further combining with protein structure prediction and rational design point mutation optimization, the cl1 mutant with higher editing efficiency is obtained, and the method can be applied to accurate and effective editing of plant genomes and plant breeding and improvement.

The invention relates to the technical field of plant breeding, in particular to a primer combination for detecting a regulatory element and an exogenous gene of a transgenic plant and application of the primer combination. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. 22-63. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. The application comprises: (1) transgenic plant variety germplasm supervision or transgenic plant component screening in non-transgenic varieties; (2) screening transgenic ingredients of the plant product; (3) auxiliary screening in the plant breeding process; and (4) tracing plant varieties. The invention provides a primer combination which can be applied to detection of regulatory elements and exogenous genes of transgenic plants, can realize detection of a plurality of regulatory elements and exogenous genes in a reaction system, and can meet high-throughput screening of components of the transgenic plants. The method is used for monitoring illegal planting of transgenic plants and detecting transgenic components of related plant products.

The invention relates to the technical field of biology, and discloses application of PHR2 in regulating nodulation and nitrogen fixation processes of leguminous plants under phosphorus starvation stress, and the application is any one of the following applications: P1, application in enhancing nitrogen fixation capability of the leguminous plants under the phosphorus starvation stress; p2, application in promoting formation of root nodules with nitrogen fixation capacity of leguminous plants under phosphorus starvation stress; p3, application of improving activity of nitrogenase in leguminous plant root nodules under phosphorus starvation stress; p4, application in leguminous plant breeding. The PHR2 protein and related biological materials thereof can be used for regulating and controlling the nitrogen fixation capacity of leguminous plants under phosphorus starvation stress.

The invention discloses application of ZmCOP1a and ZmCOP1b proteins and coding genes of the ZmCOP1a and ZmCOP1b proteins in regulation and control of plant yield. The invention belongs to the technical field of biology, and particularly relates to application of ZmCOP1a and ZmCOP1b proteins and coding genes thereof in regulation and control of plant yield. The proteins ZmCOP1a and ZmCOP1b or expression substances for regulating genes or substances for regulating the activity or content of the proteins can be applied to any one of the following applications: U1) application in regulating the plant yield; the invention also relates to application of the U3) in preparation of products for regulating and controlling plant yield, application of the U3) in preparation of products for regulating and controlling plant yield, application of the U4) in preparation of products for cultivating plants with changed plant yield, and application of the U5) in plant breeding.

The invention discloses a wheat heat-resistant protein TaHT-H8 as well as a coding gene and application thereof. The invention provides a protein TaHT-H8, a substance for up-regulating, enhancing or improving the expression of a coding nucleic acid of the protein TaHT-H8, or any one of the following applications of a substance for up-regulating, enhancing or improving the activity or content of the protein TaHT-H8: A1) improving the heat resistance of a plant; the heat resistance of the plant with the TaHT-H8 protein coding gene overexpressed is obviously higher than that of a wild type plant, and the TaHT-H8 protein coding gene can be used for industrial production and plant breeding.

The application discloses application of VvERF105 and VvSWEET15 promoter interaction in reducing sugar accumulation in fruits, and belongs to the technical field of biotechnology, and particularly relates to application of VvERF105 and VvSWEET15 promoter interaction in reducing sugar accumulation in fruits.The protein VvERF105 can interact with the VvSWEET15 promoter, the expression amount of the VvERF105 is regulated, the contents of grape glucose and fructose in grape fruits are negatively regulated, and the application prospect is good in the field of plant breeding.

The invention relates to the technical field of plant breeding, in particular to a mutant of an FZP gene promoter and application of the mutant. Compared with a wild type rice FZP gene promoter, the mutant has any one of the following mutations: (1) 5bp is deleted from 3995bp to 3989bp at the upstream of an initiation codon; and (2) inserting 1bp between 3991bp and 3990bp at the upstream of the initiation codon. The application comprises the following steps: i) increasing the grain density, the spikelet number per ear, the secondary branch number or the yield of the oryza plants; ii) cultivating a transgenic Oryza plant; the FZP gene promoter mutant has the advantages that the FZP gene promoter mutant can be used for regulating and controlling the stem differentiation process of rice, promoting the increase of the grain density of the rice and increasing the yield of the rice, and the FZP gene promoter mutant has an important application value.

The application of a soybean GmGASA1 gene, the application relates to the application of a gibberellin regulation related protein GmGASA1 coding gene of soybean.The application of the soybean GmGASA1 gene, namely the application of improving the protein content, the seed amino acid content and the grain weight of plants; the application of the soybean GmGASA1 gene in plant breeding; the application of the soybean GmGASA1 gene in cultivating transgenic plants.The experimental results show that three transgenic lines are obtained by overexpressing the GmGASA1 gene in soybean, the protein content and the grain weight of the transformed soybean seeds are significantly improved compared with the seeds of the untransformed receptor plants, which indicates that the GmGASA1 gene can regulate the protein content, the seed amino acid content and the grain weight of seeds.The GmGASA1 gene can be used for improving the quality of soybean and increasing the yield of soybean.The application of the GmGASA1 gene can effectively realize the breeding of new soybean varieties and the creation of new germplasm.

The invention relates to the technical field of plant breeding, in particular to an asexual high-yield coffee breeding method which comprises the following steps: screening superior plants to obtain a core stock plant, and preparing an asexual propagation material based on the core stock plant; carrying out seedling culture on the vegetative propagation material to obtain primary clonal seedlings, and carrying out seedling stage identification on the primary clonal seedlings to obtain healthy clonal seedlings; performing a field plot test on the healthy clonal seedlings to obtain candidate clones, and performing a multi-point regional test on the candidate clones to obtain stable clones; according to the method, asexual propagation technical parameters are optimized, composite hormone treatment is combined with a temperature control intermittent spraying technology for cutting seedling raising, and the rooting rate is larger than or equal to 80%; the tissue culture technology adopts an optimized culture medium formula, the propagation coefficient is greater than or equal to 5 times, the consistency is greater than or equal to 99%, and the problems of low propagation coefficient and low rooting survival rate of the traditional asexual propagation technology are solved.

This invention belongs to the field of plant breeding and cultivation regulation technology, and provides a method for accelerating flowering and fruiting of awnless bromegrass indoors and its application. The method includes: (1) seedling growth stage: awnless bromegrass seedlings are placed under long-day and high-temperature conditions for cultivation; (2) short-day induction stage: awnless bromegrass seedlings cultivated in step (1) are transferred to short-day and low-temperature conditions for induction cultivation; (3) long-day flowering stage: plants induced in step (2) are transferred to long-day and high-temperature conditions for continued cultivation to induce flowering and fruiting of plants and the formation of mature seeds. The method of this application is applicable to the regulation of flowering period of awnless bromegrass, hybridization and generation propagation, and provides a powerful tool for its breeding and research.

This artificial intelligence-based pollen recognition and counting system is designed to recognize and count healthy, unhealthy, and dead pollen in microscopic images with high accuracy and efficiency. This system uses a convolutional artificial neural network method that can detect objects in images quickly, precisely, and lightly. This system also has interactive software created with the Python programming language and the streamlit framework, which can display and store the results of pollen recognition and counting. This system is created by collecting, labeling, and augmenting pollen data, training and evaluating an artificial intelligence model with accuracy and error metrics, and integrating the model into the software. This system is useful for helping plant breeders optimize the crossing process.By utilizing this system, plant breeders can determine the quality and quantity of pollen more accurately and effectively, this is an effort to reduce the number of rejected seeds, which affects the productivity and sustainability of plantations.

The present application relates to the technical field of plant breeding, and in particular to a mutation breeding method for Phyllostachys edulis based on ARTP. The mutation breeding method comprises: using atmospheric room temperature plasma to treat the seeds of Phyllostachys edulis, and the parameters include: radio frequency power of 200-400 W, radiation distance of 2-3 mm, and treatment time of 8-55 minutes. The present application studies a mutation breeding method suitable for Phyllostachys edulis, and through a specific degree of ARTP mutation of the seeds of Phyllostachys edulis, the number of tillers of the seedlings of Phyllostachys edulis can be increased and the yellowing rate of the seedling of Phyllostachys edulis can be reduced under the condition of ensuring a certain seedling rate. The mutation breeding method provided by the present application can be used for breeding Phyllostachys edulis varieties with excellent traits, and has important value in the field of Phyllostachys edulis breeding.

The application discloses a corn kernel size related protein SSRP1 and application thereof. The technical problem solved is how to regulate the size of corn kernels. Specifically disclosed is the application of a protein, a substance for regulating the expression of a coding gene of the protein, or a substance for regulating the activity or content of the protein in regulating the size of plant kernels and / or preparing a product for regulating the size of plant kernels and / or plant breeding and / or preparing a plant breeding product, the protein being a protein as shown in sequence 3 in the sequence listing. The application provides a new genetic resource for the study of the size of plant kernels, in particular corn kernels, and will play an important role in the application in the field of corn seed production and breeding.

This application discloses the application of PR-1B protein in tobacco plant breeding, including the following steps: detecting the expression level of PR-1B protein in tobacco plants infected with black shank; when the expression level of PR-1B protein is overexpressed, the tobacco plant exhibits black shank resistance; the amino acid sequence of PR-1B protein is shown in SEQ ID No:1. The PR-1B protein disclosed in this application can be used reliably and sensitively to detect tobacco resistance to black shank, and provides a novel approach for screening black shank-resistant tobacco varieties and assisting traditional hybridization breeding, making it suitable for large-scale application.

The utility model relates to the technical field of seed screening, in particular to a seed screening device for garden plant breeding, which is characterized in that seeds needing to be screened are quickly blown to a screening device through a seed feeding device, screening operation is performed by matching with the screening device, and screening nets with different pore diameters are quickly switched through the screening device. Therefore, the equipment can screen seeds with different particle sizes in batches, the screened seeds are conveyed through the conveying device, and the practicability of the equipment is improved; comprising a seed feeding device, a screening device and a conveying device, the screening device is installed on the seed feeding device, and the conveying device is installed on the seed feeding device.

The present application relates to the technical field of plant breeding, in particular to StERF1A Application of a gene related biological agent in improving the resistance of potato to bacterial wilt, the biological agent StERF1A The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The present application discloses for the first time StERF1A As a negative regulatory factor involved in the mechanism of potato resistance to bacterial wilt: overexpression StERF1A Can increase the accumulation amount of bacterial wilt in leaves, inhibit the expression of disease resistance related genes Pto 、 PR1b1 And PR1a The content of active oxygen ROS and the activity of related enzymes SOD, POD and CAT are reduced; and the expression of down-regulation StERF1A The opposite effect is presented, which significantly enhances the resistance of potato to bacterial wilt. The present application provides an important gene target and theoretical basis for breeding new potato varieties with high resistance to bacterial wilt.

The invention relates to the technical field of biological information, and particularly discloses a multi-omics genome combined genetic assessment method and device. According to the method, multi-source data such as a genome, a transcriptome, a proteome, a metabolome, a microbiome and a high-throughput phenotype are integrated by constructing a multi-omics fusion similar matrix, an adaptive dichotomy is adopted to optimize omics weights, a grid search and cross validation system is introduced, and global optimal estimation of parameters is achieved. Verification of the method on livestock and poultry breeding and plant breeding data shows that the prediction accuracy of complex characters can be remarkably improved, and particularly, the prediction precision in low heritability characters is remarkably improved. The invention further provides a corresponding device which comprises a data acquisition module, a matrix construction module, a parameter optimization module and a breeding value calculation module, comprehensive utilization of multi-omics information can be efficiently achieved, and the accuracy and calculation efficiency of breeding value estimation are improved.

The invention relates to a pepper promoter CanASP28 and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the pepper promoter CanASP28 is shown as SEQ ID NO.5, and the pepper promoter CanASP28 can drive specific expression of anther in plants, can be used for creating male sterile lines, crop hybrid seed production and crop crossbreeding, can improve the seed purity and hybrid seed production and crossbreeding efficiency, and can be applied to the field of plant breeding. Therefore, an effective tool gene is provided for anther specific expression regulation and pollen expression regulation of pepper and other solanaceae crops.

This invention relates to the field of plant breeding. Particularly, the present invention relates to a diploid dicotyledonous crop plant comprising in its genome a variant of the DMP gene capable of inducing maternal haploid embryo formation and an endogenous dominant marker gene. The present invention further relates to a method for identifying and / or selecting maternal haploid dicotyledonous crop plants from a plurality of dicotyledonous crop plants comprising determining the absence of the phenotype associated with a dominant marker gene, a method for producing a maternal haploid plant comprising the step of pollinating a plant with the pollen of a diploid dicotyledonous crop plant according to the present invention, a method for producing a plant from the maternal haploid plant provided by the present invention and the use of the diploid dicotyledonous crop plant according to the present invention as a pollinator plant.

The invention relates to the field of plant breeding, in particular to application of a GhEIN3dD gene in promoting low potassium stress tolerance of plants, a recombinant vector, a recombinant microorganism, a method for improving the low potassium stress tolerance of the plants and a plant breeding method. According to the application of the GhEIN3dD gene in promoting the low potassium stress tolerance of the plant, the GhEIN3dD gene in the plant is over-expressed to improve the low potassium stress tolerance of the plant and / or improve the potassium ion absorption capacity of the plant, and the nucleotide sequence of the GhEIN3dD gene is shown as SEQ ID NO: 1. According to the application of the GhEIN3dD gene in promoting the low potassium stress resistance of the plant provided by the embodiment of the invention, the low potassium stress resistance of the plant can be improved in a low potassium environment in which a trace amount of K < + > is exogenously added, and the growth of the plant is remarkably promoted.

The present application relates to the technical field of plant breeding, and discloses a wild chrysanthemum standardization propagation method, which comprises the following steps: selecting a wild chrysanthemum single plant, establishing a seed orchard, carrying out 'water-soluble gel incubation' sowing, ecological cultivation and harvesting. The present application significantly improves the medicinal material production efficiency in the natural distribution area of wild chrysanthemum, improves the germination rate, seedling emergence rate and seedling emergence uniformity of wild chrysanthemum, greatly meets the product consistency required by large-scale production, and improves the product excellent rate.

The present application relates to the technical field of plant breeding, and discloses a method for evaluating the fertility of a wolfberry parent based on pollen activity, which comprises establishing a time sequence of wolfberry pollen sampling, obtaining pollen microscopic images at each time point and performing adaptive white balance normalization processing, identifying effective active individuals based on the saturation characteristics of the HSV color space of the corrected images and forming a preliminary activity decay sequence, fitting the preliminary activity decay sequence using a constrained Weber model to extract shape parameters, correcting the contribution weight of sub-active particles using the weight coefficients determined by the shape parameters, generating a final activity decay data sequence, calculating the pollen activity half-life based on the sequence, determining the storage resistance grade by comparing with a preset storage resistance grading standard, and generating an evaluation result. The present application corrects the evaluation weight through kinetic parameter feedback, eliminates the false positive interference of environmental light and staining, realizes the quantitative grading of wolfberry pollen storage resistance, and accurately guides the selection and matching of breeding parents.

The application provides application of RcSAPK2 gene in regulating drought resistance of plants and a method for regulating drought resistance of plants. In a first aspect, the application provides application of RcSAPK2 gene in regulating drought resistance of plants, wherein the RcSAPK2 gene is one of the following: 1) a nucleic acid molecule with a nucleotide sequence as shown in SEQ ID NO. 1; 2) a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO. 1 through substitution, deletion or addition of one or more nucleotides; and 3) a nucleotide sequence with at least 80% identity with SEQ ID NO. 1. Experiments show that the RcSAPK2 gene provided by the application plays an important role in resisting drought stress of plants, and provides an important basis for plant breeding.