1280 results about "Plant breeding" patented technology

The current invention provides the promoter of the Zea mays nuclear gene encoding chloroplast-localized fructose-1,6-bisphosphate (F16BP) aldolase. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the chloroplastic F16BP aldolase promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.

Methods and compositions relating to glyphosate resistant maize plants, including the GA21, GG25, GJ11 and FI117 transformation events, are disclosed. Also disclosed are methods of using herbicide resistance transformation events in plant breeding procedures. The invention further includes methods of ensuring plant seed purity.

The current invention provides regulatory regions from the rice actin 2 gene. In particular, the current invention provides the rice actin 2 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the rice actin 2 intron and / or promoter directly by genetic transformation, as well as by plant breeding methods. The actin 2 sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Methods for using genetic marker genotype (e.g., gene sequence diversity information) to improve the process of developing plant varieties (e.g., single cross hybrids) with improved phenotypic performance are provided. Methods for predicting the value of a phenotypic trait in a plant are provided. The methods use genotypic, phenotypic, and optionally family relationship information for a first plant population to identify an association between at least one genetic marker and the phenotypic trait, and then use the association to predict the value of the phenotypic trait in one or more members of a second, target population of known marker genotype. Methods for identifying new allelic variants affecting the trait are also provided. Plants selected, provided, or produced by any of the methods herein, transgenic plants created by any of the methods herein, and digital systems for performing the methods herein are also provided.

This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.

Methods for classifying plants by remote sensing and image analysis technology are presented. These methods are useful for evaluating plants and for selecting plants for a plant breeding program which has as its goal to selectively alter phenotype. The methods combine the newer techniques of remote sensing technology to obtain indirect correlates of the traits of interest, with classical pedigree breeding strategies. Thermal and infrared reflectance measures of plant canopies are examples of energy values measured by remote sensing, used to indirectly predict the selected traits.

The present invention is in the field of plant biochemistry and genetics. More specifically the invention relates to nucleic acid molecules from plant cells, in particular, genomic DNA sequences from rice plants and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins, and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression, and transgenic plants.

The current invention provides the maize RS81 promoter. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the RS81 promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Powder type culture medium is from plenty of element kinds , trace element and iron salt and vitamin kind of composition, after processing manufacture the original powder that makes powder culture medium. The various components of the medium and the final product were dry powder. The preparation Method: First, concentrate all plenty of elements to collect reserve; Mix trace element and vitamin kind totally to wrap to attach , and then mix the thin medicine and plenty of elements of trace element totally, under the role with centrifugal high speed, making it even scatter and wrap up to glue to unite. So, make the original powder of powder culture medium for manufacture. This medium-dry powder manufacturing easier, does not require the use of complex machines and equipment, and low-cost easy to promote. The product is of high quality in existing product. It can be used in plant breeding, tissue culture and molecular biology research, and can be used for various commercial purposes.

The present invention provides breeding methods and compositions to enhance the germplasm of a plant. The methods describe the identification and accumulation of transgenes and favorable haplotype genomic regions in the germplasm of a breeding population of crop plants.

The invention discloses a thread-guiding-type pepper selfing fruit marking method and belongs to the technical field of plant breeding. The thread-guiding-type pepper selfing fruit marking method comprises the following steps: firstly selecting selfing single plants, and marking by hanging a tag; secondly, selecting flowers about to bloom in the selected selfing single plants; threading a filament into a hand sewing needle with a certain specification (0.2-0.4mm), and tieing a knot at one end close to a needle hole; threading a guiding thread of the hand sewing needle through a pedicel part of each flower to be bloomed; leaving the tail end, about 2-3cm long, of the filament into the pedicel part as a marker of a selfing pollination flower; manually pollinating the marked flowers; and harvesting fruits with filament markers in the pedicel parts in a physiological mature period.

The present invention relates generally to an apparatus for and a method of measuring and selecting grain for use in milling, or seed for use in plant breeding. Said method is adapted to optically analyze seeds / grains to qualitatively and quantitatively characterize the seed / grain, and more particularly, to analyze the gradation of color, whiteness, and hard endosperm of the seed / grain. This method and apparatus perform color image analysis of seed / grain sample(s) to characterize multiple quality traits.

A method for screening for a trait associated with the altered expression of a gene of interest in plants is provided. The method is a combinatorial approach which uses traditional plant breeding techniques for modifying the patterns of expression of a gene of interest. Kits for use in the method and transgenic plants generated by the method are also provided.

The invention relates generally to an improved plant breeding system. More particularly, this invention relates to a method for automated, high throughput analysis of plant phenotype and plant genotype in a breeding program.

A method for plant breeding by identifying, balancing, and combining multiple traits is provided. The method enhances the profitability of a hybrid corn seed production system by providing traits having high impact on decreasing cost of production on female parent and traits having low impact on decreasing cost of production and end users traits on the female and / or the male parent. The invention also provides a hybrid corn seed produced by such method.

The invention relates to a rice field-fishpond planting and breeding compound system, which belongs to the technical field of comprehensive utilization of agricultural ecological aquaculture. The compound system comprises a rice field, a fishpond, a simple ecological ditch and a strengthened ecological ditch, wherein the rice field is arranged at one end of the fishpond; the strengthened ecological ditch and a water collecting tank are respectively arranged on one side of each of the fishpond and the rice field; the simple ecological ditch is formed in the rice field; a small overflow port is formed in the simple ecological ditch; the rice field is communicated with the water collecting tank by a large overflow port; the fishpond is communicated with the simple ecological ditch by a water suction pump. According to the rice field-fishpond planting and breeding compound system disclosed by the invention, the rice field planting and the fishpond breeding are organically combined by the ecological ditches, so that the fishpond breeding water is refluxed to a new circulating water planting-breeding mode after being purified by the rice field and the ecological ditch. Moreover, the problems that an existing rice-orientated planting-breeding mode with the fish breeding has rice-fish contradictions including field drying, fertilizer applying, pest controlling and the like in the production process, and is limited in yield and inconvenient for large-area popularization are solved.

The invention belongs to the technical field of plant breeding, and in particular relates to a single flower selfing isolation method for small chili. The method comprises the following steps: (1) selecting an excellent single plant, namely in the flowering period, selecting the excellent single plant according to a breeding objective, and marking; (2) preparing a capsule shell, namely separating a shell cap from a shell body of a hollow hard capsule shell; (3) selecting a flower bud, namely selecting a large flower bud which is expected to bloom in the next day; (4) treating the flower bud, namely holding the flower bud to be treated by one hand, holding the capsule shell, which is matched with the flower bud in size, by the other hand, slightly sleeving the flower bud with the lower opening of the capsule shell over the widest part of the flower bud, and marking a flower stalk; (5) managing after the treatment in a method which is the same as a conventional field management method for the production of small chili. The capsule shell used in the method is low in cost and does not hurt the flower bud and the flower stalk when being used, the operation is convenient and easy, and the work efficiency is high; moreover, the capsule shell with certain elasticity and permeability does not influence the cracking of anther and pollination, and thus the selfing setting rate of the small chili is high.

The present invention provides novel methods and compositions for the identification and selection of loci modulating transgene performance and expression in plant breeding. In addition, methods are provided for screening germplasm entries for the performance and expression of at least one transgene.

The present invention relates to the field of plant breeding. More specifically, the present invention includes a method of using haploid plants for genetic mapping of traits such as disease resistance. Further, the invention includes a method for breeding corn plants containing quantitative trait loci (QTL) that are associated with resistance to Gray Leaf Spot (GLS), a fungal disease associated with Cercospora spp. The invention further includes a method for breeding corn plants containing QTL that are associated with Goss' Wilt, a bacterial disease associated with Clavibacter michiganense spp.

The present invention is in the field of plant biochemistry and genetics. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, genomic DNA sequences from Arabidopsis thaliana plants. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins, and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression, and transgenic plants.

The invention relates to an artificial mass propagation method of harmonia axyridis. Two facilities of a seedling raising greenhouse and an insectary are adopted, broad beans are used as host plants for propagating pea aphids which are used as aphids for feeding the harmonia axyridis, and the harmonia axyridis which is used for agricultural control over the aphids can be manually propagated in a large quantity through specific technical measures of broad bean plant breeding, pea aphid inoculation and propagation, artificial breeding of the harmonia axyridis and the like. Healthy broad bean sprouts are bred with broad bean seeds, the pea aphids are inoculated with the broad bean sprouts and propagated, then the pea aphids are collected to feed larvas and adults of the harmonia axyridis, and a large quantity of the larvas and the adults of the harmonia axyridis are propagated to be released in agricultural production areas to control the aphids of all kinds, so that the biological control over the aphids is realized, and the pollution and safety problems caused by chemical control is avoided or reduced.

The present invention is in the field of plant biochemistry and genetics. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, genomic DNA sequences from Arabidopsis thaliana plants. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins, and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression, and transgenic plants.

The invention relates to a submerged plant breeding blanket and application thereof. The submerged plant breeding blanket disclosed by the invention is respectively composed of a carrier layer, a matrix layer and an adhesive layer from bottom to top, wherein the carrier layer is composed of palm and bamboo plant fiber; the matrix layer is composed of a growing matrix suitable for submerged plants to grow; and the adhesive layer is composed of a plant fiber net. The invention further provides a submerged vegetation transplantation technology which is characterized in that after being subjected to seedling culture, submerged plant seedling breeding embryoid bodies prepared by virtue of the breeding blanket are transplanted to the water bottom of an ecological restoration area for planting and restoring submerged vegetation of the ecological restoration area. Compared with the traditional submerged plant transplantation technology method, the submerged vegetation transplantation technology disclosed by the invention has the advantages of low transplantation cost, high speed, high survival rate, convenient technology, strong technical maneuverability, high community forming speed after transplantation, high breeding speed, good community stability and the like, and can be used for realizing the quick transplantation and the community construction and configuration of the submerged plants.

The present invention is in the field of plant biochemistry and genetics. More specifically the invention relates to nucleic acid molecules from plant cells, in particular, genomic DNA sequences from rice plants and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins, and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression, and transgenic plants.

The invention discloses rice herbicide resistant protein and application thereof in plant bleeding. The ALS (acetolactate synthase) protein provided by the invention realizes that a plant has the herbicide resistance, and is derived from rice varieties Huangsizhan and Huanghuazhan, and carries Trp548, Ala96 or Ser627mutation. Furthermore, the invention also provides corresponding nucleic acid, expression cassettes, carriers, cells, plants, and method for obtaining the plants with herbicide resistance and application of the plants, as well as a method for identifying the plants in the invention.