606 results about "Plant breeding" patented technology

The invention relates to the field of biotechnology and plant breeding, and particularly provides a method for fixing rice heterosis by using a DMP gene. Aiming at the problems of low maturing rate and complex operation of the existing apomixis system, the haploid induction functions of the rice OsDMP8 and OsDMP13 genes are verified for the first time, the OsPAIR1, OsREC8, OsOSD1 and OsDMP genes are synchronously knocked out through a CRISPR / Cas9 technology, a'MiMe-OsDMP 'four-gene editing system is constructed, and a new thought is provided for construction of the rice apomixis system. A new solution is provided for fixing heterosis of rice apomixis, the efficiency and quality of agricultural production can be greatly improved, and the method has high economic value and wide application prospects.

A resistance gene TaCAT to FCR of wheat and an application thereof are provided, which relates to the field of biological genetic engineering technologies. The nucleotide sequence of a genome where the TaCAT gene is located, the nucleotide sequence of a cDNA, the nucleotide sequence of a CDS and a coded protein sequence are respectively as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. An InDel marker is developed for identifying different allelotypes of the TaCAT gene. An application of the TaCAT gene for preparation of transgenic plant cells and plant breeding is provided. The molecular genetic basis of the resistance to FCR is disclosed, an important role is played in improving the resistance to FCR by utilizing a genetic engineering technology, and a new way is provided for breeding a new wheat variety with disease-resistance and safety.

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention provides a salt-tolerant plant constructed wetland system for treating eutrophicated mariculture wastewater, which specifically comprises a lower filler formed by mineral materials, and a middle filler formed by modified zeolite, sodium alginate coated vermiculite, rice husk charcoal, oyster shell, coral sand and diatomite, the surface filler is composed of intertidal zone bottom mud, intertidal zone raw water, coastal saline-alkali soil and composite algae powder, and the three fillers are combined with salt-tolerant plant breeding, bow-shaped water flow optimization and layered collaborative design; the core problems of poor salt tolerance, low adsorption efficiency, easiness in blockage, high maintenance cost and the like of the traditional constructed wetland during treatment of the mariculture wastewater are systematically solved.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention relates to a gene for regulating and controlling the grain number of crops and application thereof. Specifically, the invention provides an application of GNR1 (Grain Number in Rice1) or an allele of the GNR1 in Rice1 in the aspect of improving the yield of rice by regulating and controlling the change of the grain number per spike of the rice. Also disclosed are methods of growing plants with increased grain number per ear, increased stem number and / or increased yield. The GNR1 gene has a wide application prospect in plant breeding.

The invention discloses application of a corn ZmTST1 gene in improvement of plant yield and stress resistance. The nucleotide sequence of the corn ZmTST1 gene is as shown in SEQ ID NO. 1; the stress resistance is tolerance to salt stress and drought stress. Through sequence analysis and functional verification, after the ZmTST1 gene is over-expressed, the growth of plants can be promoted, the yield of the plants can be increased, meanwhile, the drought tolerance and the salt tolerance of ZmTST1 expressed plants are obviously higher than those of wild plants, and it is proved that the ZmTST1 gene has a remarkable effect in the aspects of high yield and stress resistance. By identifying the ZmTST1 gene, plants with high stress resistance can be screened, the cultivation period of the plants can be shortened, and a new method and thought are provided for plant breeding in the future.

The invention relates to the field of plant breeding, in particular to application of a GhZAT10 gene in promoting low potassium stress tolerance of plants, a recombinant vector, a recombinant microorganism, a method for improving the low potassium stress tolerance of the plants and a plant breeding method. According to the application of the GhZAT10 gene in promoting the low potassium stress tolerance of the plant, the GhZAT10 in the plant is over-expressed to improve the low potassium stress tolerance of the plant and / or improve the potassium ion absorption capacity of the plant, and the nucleotide sequence of the GhZAT10 is shown as SEQ ID NO: 1. According to the application of the GhZAT10 gene in promoting the low-potassium stress resistance of the plant, the low-potassium stress resistance of the plant can be improved in a low-potassium environment in which K < + > is not added and a trace amount of K < + > is added in an external source, and the growth of the plant is remarkably promoted.

The present invention relates to the fields of biotechnology and plant breeding, specifically providing a method for fixing heterosis in rice using the DMP gene. To address the low fruit set rate and complex operation issues of existing apomictic reproduction systems, the present invention verifies for the first time the haploid-inducing function of the rice genes OsDMP8 and OsDMP13. Furthermore, the CRISPR / Cas9 technology is used to simultaneously knock out the OsPAIR1, OsREC8, OsOSD1, and OsDMP genes, constructing a "MiMe-OsDMP" four-gene editing system. This system provides new ideas for constructing apomictic reproduction systems in rice and offers a new solution for fixing heterosis in rice apomictic reproduction. This system can significantly improve the efficiency and quality of agricultural production, and has significant economic value and broad application prospects.

The invention discloses application of TaNOP2 protein and a coding gene thereof in wheat breeding. The invention also protects the application of the TaNOP2 protein or the TaNOP2 gene as an inhibition target in plant breeding. The target of the plant breeding is to cultivate plants with increased plant height and / or yield and / or tillering number and / or spike length and / or spike weight and / or grain number per spike and / or thousand grain weight and / or photosynthetic rate and / or grain length and / or grain width and / or grain perimeter and / or grain surface area. The invention provides a new thought and a potential target for wheat molecular breeding, can be used for cultivating high-yield new wheat germplasm, and has industrial popularization value.

The invention discloses a grape downy mildew resistance whole genome selective breeding method based on machine learning, which comprises the following steps: randomly dividing 132 samples into a training set and a test set according to a ratio of 4: 1, and respectively extracting first 1-100 thousand variation sites as gradient training data sets according to a whole genome association analysis result; and on the basis of 10 classic regression models and the dimensionality-reduced variation data set, machine learning training and evaluation are carried out on 105 training set samples, finally, the variation data set with the highest prediction accuracy and an optimal model are screened out, and phenotype prediction is carried out on 27 test set samples by using the optimal model. The invention relates to the technical field of plant breeding, and the grape downy mildew resistance whole-genome selective breeding method based on machine learning has remarkable advantages in resistance prediction and screening, breeding efficiency improvement and multi-character comprehensive improvement, breaks through the limitation of a traditional breeding method, and is suitable for large-scale popularization and application. An innovative solution is provided for green and sustainable development of the grape industry.

The invention discloses a SiERF109 gene for regulating drought resistance, salt tolerance and flavonoid accumulation of millet and application of the SiERF109 gene, and belongs to the technical field of plant breeding. According to the invention, a gene SiERF109 with a nucleotide sequence shown as SEQ ID NO.1 is separated and cloned from millet, and fluorescent quantitative expression analysis shows that SiERF109 participates in drought and salt stress response of millet. After the gene is over-expressed in foxtail millet, in drought and salt stress experiments, compared with a wild type strain, the survival rate and chlorophyll content of a strain over-expressed with the SiERF109 gene are remarkably increased, the flavonoid content is increased by 33.5%-39.5%, and active oxygen accumulation is remarkably reduced; it is shown that the SiERF109 gene and the protein coded by the SiERF109 gene can remarkably improve the drought resistance, the salt resistance and the flavonoid content of the plant.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

An epigenetic regulatory factor ZmULT1 related to corn grain size regulation and heat stress response and the use thereof, which solve the technical problems of how to increase the grain size and / or heat resistance and / or lodging resistance of corn. Use of a substance for reducing the content of the protein ZmULT1 or a substance for inhibiting the expression of the encoding gene of the protein ZmULT1, which use is any one of the following: P1, the use in increasing the grain size of corn; P2, the use in improving the heat resistance of corn; P3, the use in increasing the lodging resistance of corn; and P4, the use in plant breeding. The homozygous mutant strain with knockout of the ZmULT1 gene has a reduced plant height and an increased grain size, and exhibits significant heat stress resistance, this indicates that the research on the gene can further enrich the use of epigenetics in crops and is of important significance for improving the grain size, heat resistance and lodging resistance of corn.

The invention provides application of a tobacco NtMYC1 gene in promoting growth and development of tobacco glandular hair, and belongs to the technical field of plant breeding. The tobacco MYC1 transcription factor is obtained by carrying out sequence characteristic analysis, conservative structural domain identification, protein physicochemical property analysis and phylogenetic analysis on a genome of tobacco. And an RNAi technology and an overexpression tobacco transgenic line verify that the tobacco MYC1 transcription factor and the coding gene thereof have the effect of regulating and controlling the growth and development of the tobacco glandular hair, and the growth and development of the tobacco glandular hair are promoted through forward regulation and control, so that the growth and development of the tobacco glandular hair are inhibited through silent expression. Therefore, the tobacco MYC1 transcription factor and the coding gene thereof provided by the invention provide a new means for tobacco breeding.

The invention discloses an application of a protein TabHLH92 in regulation and control of wheat tillering. The invention belongs to the technical field of biology, and particularly relates to application of protein TabHLH92 to regulation and control of wheat tillering. The protein TabHLH92 is a protein with an amino acid sequence of SEQ ID No: 2, SEQ ID No: 4 or SEQ ID No: 6, is a protein for negatively regulating the tillering ability of wheat, can be applied to regulation of the tillering ability of plants and cultivation of plants with changed tillering ability, and also has a wide application prospect in the field of plant breeding.

The invention discloses a polypeptide and application thereof in regulation and control of plant development, and relates to the technical field of biology. The invention specifically discloses any polypeptide from A1) to A3) as shown in the following: A1) a polypeptide with an amino acid sequence as shown in SEQ ID NO: 1; a2) a polypeptide which is obtained by substitution and / or deletion and / or addition of more than one amino acid residue on the amino acid sequence as shown in SEQ ID NO: 1 and has the function of regulating plant growth and development; and A3) a fused polypeptide obtained by connecting a tag to the N end and / or C end of A1) or A2). The polypeptide can effectively regulate and control root system growth and root regeneration of plants, can be well applied to improvement of plant varieties, and has important practical value for plant breeding work.

The invention relates to the field of bioinformatics, in particular to a genome selection method, device and equipment, a medium and a computer program product. Integrating a plurality of machine learning models, including 13 algorithms such as a support vector machine (SVM), linear regression, Ridge regression, Lasso regression and the like; by combining automatic hyper-parameter optimization, parallel computing and efficient data preprocessing technologies, the breeding prediction precision and the computing efficiency are remarkably improved. The device comprises an IASML software module and an IASML cloud platform module, wherein software supports command line operation and is suitable for large-scale data calculation; the cloud platform provides a graphical interface and supports online data uploading, model selection, real-time monitoring and result downloading. According to the method, the problems of singleness, limited data scale, poor interactivity and the like of an existing tool model are solved, and an efficient, flexible and reproducible intelligent solution is provided for animal and plant breeding and genetic research.

The present disclosure provides the use of β-1,3-glucan synthase like 5 (GSL5) for the improvement of the clubroot resistance in cruciferous crops as well as related products development, belonging to the area of molecular engineering for plant breeding. GSL5 gene is highly conserved in cruciferous plants and mutation of GSL5 can confer a broad-spectrum and high clubroot resistance to the cruciferous crops, demonstrating that molecular engineering of GSL5 can achieve the improvement of the clubroot resistance in cruciferous crops. The present disclosure provides a key gene and techniques for the improvement of the clubroot disease resistance and durable prevention and control of the clubroot disease in cruciferous crops.

The invention discloses application of protein TaTDM and related biological materials thereof, and belongs to the field of plant breeding. The amino acid sequence of the protein TaTDM is as shown in SEQ ID NO: 2 or SEQ ID NO: 4 or SEQ ID NO: 6. The TaTDM gene knockout homozygous mutant strains tatdmm1 and tatdmm2 are obtained by carrying out gene knockout on wild type wheat Fielder, and it is found that at the temperature of 25 DEG C, the total floret number of each spike of the Fielder, the tatdmm1 and the tatdmm2 and the survival rate under the water shortage treatment condition are remarkably higher than those of the wild type wheat Fielder. The protein TaTDM has an important application value in regulation and control of wheat ear type characters and stress tolerance, provides a high-quality germplasm resource, and is of great significance in cultivation of drought-enduring plant varieties and improvement of crop yield.

The invention discloses application of a rice SD10 gene in yield regulation and lodging resistance. The invention provides a protein, a substance for up-regulating or enhancing or improving the expression of a coding gene of the protein, or an application of a substance for up-regulating or enhancing or improving the activity or content of the protein in at least one of the following substances. A1) improving lodging resistance of plants; a2) increasing the plant yield; a3) cultivating lodging-resistant plants; a4) cultivating high-yield plants; a5) plant breeding; the protein comprises an amino acid residue as shown in SEQ ID No. 2; the invention proves that the SD10 gene simultaneously affects the characters such as single plant yield and stalk thickness, and plays a very important role in regulating and controlling the rice yield and lodging resistance, and the encoding nucleic acid of the protein SD10 is a high-quality gene for cultivating high-quality variety rice, and is used for cultivating high-yield and lodging-resistant rice.

In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

The invention discloses application of a wheat nitrogen response gene TaNIA1 in cultivation of high-yield wheat. The invention belongs to the technical field of biology, and particularly relates to application of a wheat nitrogen response gene TaNIA1 in cultivation of high-yield wheat. The protein TaNIA1 disclosed by the invention can be applied to the following aspects: 1) application to regulation and control of plant yield; 2) application in preparation of products for regulating and controlling plant yield; the invention relates to application of TaNIA1 in cultivation of plants with changed yield, application of TaNIA1 in preparation of products for cultivation of plants with changed yield, and application of TaNIA1 in plant breeding. TaNIA1 influences nitrogen absorption and utilization of wheat, so that agronomic traits such as wheat tiller number, grain number per ear, ear length, hundred-grain weight and the like are remarkably increased, and the yield of wheat is finally influenced.

The invention relates to an application of a genetic transformation method of populus sibirica and a CRISPR / Cas9 gene editing method in plant breeding, and belongs to the technical field of plant molecular breeding. The method comprises the following steps: (1) soaking an explant in an infection bacterium solution; (2) spreading on a CM1 culture medium; (3) transferring the explant to a CM2 culture medium for 4-5 weeks; (4) transferring the calluses to a CM3 culture medium until adventitious buds are obtained; (5) transferring the callus with the adventitious buds to a CM4 culture medium until cluster buds are obtained; and (6) cutting off clustered seedlings, and inserting the clustered seedlings on a CM5 culture medium. According to the application of the genetic transformation and CRISPR / Cas9 gene editing method of the poplar in plant breeding, a target gene is transferred into a poplar body to obtain a positive plant, meanwhile, a poplar phytoene dehydrogenase (PDS) gene is taken as an example, a poplar mutant of which the PDS gene is knocked out is successfully obtained by applying a CRISPR / Cas9 gene editing technology, and the method is suitable for plant breeding. And a technical support is provided for application of a CRISPR / Cas9 gene editing technology in molecular breeding of the populus sibirica.

The invention belongs to the technical field of plant breeding, and particularly relates to a breeding method of high-yield multi-chamber brassica napus. Comprising the following steps: performing hybridization by taking a mustard type rape multi-chamber resource as a female parent and a cabbage type rape as a male parent to obtain an F1 generation, and performing isolated propagation to obtain F2 free seeds; selecting partial brassica napus type single plants from F2 free seeds for selfing propagation to obtain F3-generation seeds, planting the F3-generation seeds according to plant lines, performing selfing propagation, screening out single-plant selfing seeds with multi-chamber pods, continuing selfing, continuously repeating for 2-4 generations, screening out single plants closest to male parent characters, and obtaining high-yield multi-chamber brassica napus resources. The multi-chamber brassica napus resource with the multi-chamber pod rate larger than 60% is obtained through the method, the novel multi-chamber brassica napus resource with the pod length close to that of double No.11 in brassica napus parents is obtained, and the pod length of the novel multi-chamber brassica napus resource reaches 11.01 cm.

The invention belongs to the technical field of genetic engineering application and plant breeding, and particularly relates to application of a rice OsHGO gene to improvement of high-temperature tolerance of rice. The OsHGO gene in japonica rice Zhonghua 11 is knocked out through a CRISPR / Cas9 system, and a homozygous mutant plant with the OsHGO gene knocked out is obtained. The phenotypes of the wild type and mutant plants after high-temperature treatment are observed, and it is found that the wild type has more serious leaf wilt than the mutant plants. It is shown that after the OsHGO is knocked out, the influence of the high temperature on plant growth is relieved, and the OsHGO gene regulates and controls the tolerance of the rice to the high temperature. Through mutation of the gene, high-temperature-resistant rice can be cultivated.

The invention relates to a multivariable gradient linkage seedling culture cabin, and belongs to the technical field of seedling culture. Comprising a seedling culture cabin, a transfer assembly, a bearing assembly, a plurality of stress assemblies and a plurality of pneumatic partition devices. The seedling culture cabin is provided with a first partition and a second partition; the transfer assembly is located in the seedling raising cabin and is distributed in a first preset direction and a second preset direction; the bearing assembly is installed on the transfer assembly distributed in the first preset direction, plants are arranged on the bearing assembly, and the bearing assembly circulates between the first partition and the second partition; the multiple stress assemblies are located in the second subareas correspondingly and installed on the transfer assembly moving in the second preset direction; the transfer assemblies are distributed at the top and the bottom of the seedling culture cabin, and the pneumatic partition device is located on the transfer assembly at the top and used for creating an independent space in the seedling culture cabin. The method is used for creating environment data of multiple gradients or environment data of mutual superposition of multiple variables for the same variable to carry out cultivation recording during plant breeding.

The present disclosure is in the field of plant breeding. The disclosure provides methods for breeding corn plants having a brachytic trait using marker-assisted selection. The disclosure further provides brachytic germplasm, markers associated with a brachytic trait for introgressing the trait into elite germplasm in a breeding program. This disclosure also provides brachytic or dwarf elite corn varieties having yield equal to or higher than conventional non-brachytic corn varieties.