765 results about "Plant breeding" patented technology

The invention discloses an application of a TaNLP-3B gene and a related biological material thereof in regulating and controlling the protein content of plant seeds. The gene TaNLP-3B is cloned from wheat, the gene TaNLP-3B is overexpressed in wheat Fielder to obtain a TaNLP-3B transgenic overexpressed plant, and a detection result shows that the protein content in wheat seeds can be remarkably increased by more than 40% through TaNLP-3B gene overexpression, so that the TaNLP-3B gene has an effect of regulating and controlling the protein content in the plant seeds, and the protein content in the wheat seeds can be increased by more than 40%. The method can be applied to plant breeding or quality improvement.

The invention provides a Chinese cabbage clubroot susceptibility related gene BrSWEET14a, a construction method and application, belongs to the technical field of plant genetic engineering, and particularly relates to a Chinese cabbage clubroot susceptibility related gene BrSWEET14a. The CDS sequence of the Chinese cabbage BrSWEET14a is as shown in SEQ ID No. 1 (sequence identifier number 1). The gene is transformed into Col-type arabidopsis thaliana through an agrobacterium flower immersion transformation method, a BrSWEET14a heterologous expression arabidopsis thaliana strain is obtained, and the result shows that T-DNA insertion mutant of the BrSWEET14a homologous gene Atsweet14 is more resistant to clubroot than that of the Col-type arabidopsis thaliana, and heterologous expression of Chinese cabbage BrSWEET14a leads to reduction of disease resistance of arabidopsis thaliana to plasmodiophora brassicae. Therefore, the Chinese cabbage BrSWEET14a is closely related to the clubroot disease, and the gene and the homologous gene of the gene in other cruciferous plants can be applied to breeding of the cruciferous plants and other horticultural plants and have a good application prospect.

The invention relates to the field of biotechnology and plant breeding, and particularly provides a method for fixing rice heterosis by using a DMP gene. Aiming at the problems of low maturing rate and complex operation of the existing apomixis system, the haploid induction functions of the rice OsDMP8 and OsDMP13 genes are verified for the first time, the OsPAIR1, OsREC8, OsOSD1 and OsDMP genes are synchronously knocked out through a CRISPR / Cas9 technology, a'MiMe-OsDMP 'four-gene editing system is constructed, and a new thought is provided for construction of the rice apomixis system. A new solution is provided for fixing heterosis of rice apomixis, the efficiency and quality of agricultural production can be greatly improved, and the method has high economic value and wide application prospects.

A resistance gene TaCAT to FCR of wheat and an application thereof are provided, which relates to the field of biological genetic engineering technologies. The nucleotide sequence of a genome where the TaCAT gene is located, the nucleotide sequence of a cDNA, the nucleotide sequence of a CDS and a coded protein sequence are respectively as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. An InDel marker is developed for identifying different allelotypes of the TaCAT gene. An application of the TaCAT gene for preparation of transgenic plant cells and plant breeding is provided. The molecular genetic basis of the resistance to FCR is disclosed, an important role is played in improving the resistance to FCR by utilizing a genetic engineering technology, and a new way is provided for breeding a new wheat variety with disease-resistance and safety.

The invention discloses an application of a TaPKMT-5A gene and a related biological material thereof in regulating and controlling the protein content of plant seeds. The gene TaPKMT-5A is cloned from wheat, the gene TaPKMT-5A is overexpressed in wheat Fielder, a TaPKMT-5A gene overexpressed plant is obtained, and a detection result shows that the protein content in wheat seeds can be remarkably increased by 13.3%-16.9% or above through TaPKMT-5A gene overexpression, so that the TaPKMT-5A gene has the effect of regulating and controlling the protein content in the plant seeds, and the protein content in the wheat seeds can be remarkably increased by 13.3%-16.9% or above. The method can be applied to plant breeding or quality improvement.

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

The invention discloses a paphiopedilum PCMYB1 gene and application of the paphiopedilum PCMYB1 gene in changing plant flower color. The amino acid sequence of the PCMYB1 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the coding gene of the PCMYB1 is as shown in SEQ ID NO. 1. Through instantaneous transformation or stable overexpression of the gene, the synthesis level of anthocyanin in the plant can be effectively improved, so that the flower color of the plant is changed. The application provides an innovative thought for plant breeding, the ornamental value and market competitiveness of flowers can be remarkably improved, and a new growth point is brought to the flower industry. Meanwhile, the plant flower color is changed by means of genetic engineering, the biological diversity can be enriched, the increasingly diversified requirements of consumers are met, and the progress of germplasm innovation and sustainable agriculture in China is promoted.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention provides a salt-tolerant plant constructed wetland system for treating eutrophicated mariculture wastewater, which specifically comprises a lower filler formed by mineral materials, and a middle filler formed by modified zeolite, sodium alginate coated vermiculite, rice husk charcoal, oyster shell, coral sand and diatomite, the surface filler is composed of intertidal zone bottom mud, intertidal zone raw water, coastal saline-alkali soil and composite algae powder, and the three fillers are combined with salt-tolerant plant breeding, bow-shaped water flow optimization and layered collaborative design; the core problems of poor salt tolerance, low adsorption efficiency, easiness in blockage, high maintenance cost and the like of the traditional constructed wetland during treatment of the mariculture wastewater are systematically solved.

The invention belongs to the technical field of plant breeding, and particularly relates to a method for identifying salt tolerance of asparagus in the whole growth period in coastal saline-alkali soil. According to the method, the salt tolerance of the variety is identified in the germination period, the seedling period and the field growth period of the asparagus, so that the salt tolerance of the whole growth period of the asparagus is comprehensively evaluated. In the germination period and the seedling period of the asparagus, a traditional sodium chloride stress method is changed into a saline-alkali soil leaching solution for salt stress treatment. According to the method, the salt tolerance of the asparagus varieties is evaluated by adopting a fuzzy comprehensive evaluation method in the field growth period of the asparagus, the contribution rates of a plurality of phenotype indexes under salt stress are comprehensively considered, the salt tolerance correction membership function value of each variety is calculated, and the limitation of evaluation by a single character index is avoided. According to the method, the salt tolerance of the variety can be reflected more comprehensively and more accurately from different growth periods of the asparagus.

The invention belongs to the technical field of plant breeding and biology, and particularly relates to how to reduce the cadmium content of rice grains through a gene OsCT1 and application of the gene OsCT1 to creation of new germplasm of low-cadmium-accumulation rice. The invention discloses a nucleotide sequence of a gene OsCT1 coding region, a coded protein sequence, a CRISPR-Cas9 knockout target site nucleotide sequence, a nucleotide sequence of a knockout mutant and a protein sequence of the knockout mutant, as shown in an ST.26 standard sequence nucleotide or amino acid sequence table. After the gene OsCT1 is knocked out by utilizing a CRISPR-Cas9 technology, the cadmium content of grains of the OsCT1-knocked-out transgenic plant is obviously lower than that of a wild type variety. In addition, in the seedling stage, the plant height and the root length of the OsCT1-knocked-out transgenic plant are remarkably larger than those of a wild type variety; after the OsCT1 gene is subjected to overexpression, the grain cadmium content of an overexpressed transgenic plant is slightly higher than that of a wild type variety. In addition, in the seedling stage, the plant height and the root length of the overexpressed OsCT1 transgenic plant are obviously smaller than those of a wild type variety. According to the invention, the OsCT1 gene can be utilized to create a novel rice germplasm with low cadmium content in grains.

The invention discloses an application of an ABC transporter family gene OsABCA3 in regulation and control of plant breeding. Belongs to the field of plant genetic engineering. According to the application of the ABC transport protein family gene OsABCA3 in plant breeding regulation and control, the amino acid sequence of the protein encoded by the ABC transport protein family gene OsABCA3 is shown as SEQ ID No: 2. According to the invention, the ABC transporter gene OsABCA3 is knocked out in rice, so that the Cd absorption in a knockout plant under Cd stress is obviously reduced compared with that of a wild type plant, the Cd content of grains in the knockout plant planted in Cd-polluted soil is obviously reduced compared with that of the wild type plant, but the content of essential elements in the grains and the yield of rice are not negatively influenced; therefore, a new candidate gene resource is provided for creating safe rice with low Cd grains, and a potential repair method is also provided for rice Cd pollution treatment.

The invention discloses a novel corn haploid immature embryo tissue culture doubling method, and belongs to the field of plant breeding. The technical problem to be solved by the invention is how to obtain a simpler corn haploid breeding method. The corn haploid immature embryo chromosome doubling and seedling forming method provided by the invention comprises the following steps: taking a corn inbred line as a female parent, taking a corn haploid induction line as a male parent, inducing to obtain a corn immature embryo, inoculating the corn immature embryo into a double-layer culture medium, and culturing to obtain the corn haploid immature embryo chromosome doubling and seedling forming method. Obtaining seedlings formed by chromosome doubling of the corn haploid immature embryos; the double-layer culture medium is composed of a seedling culture medium and a doubling culture medium located on the seedling culture medium. According to the method provided by the invention, chromosome doubling and seedling formation of the maize haploid immature embryos can be completed in one step, and the maize haploid immature embryos do not need to be transferred to a seedling formation culture medium after chromosome doubling, so that a large amount of manpower is saved, and the method is expected to be used in production practice.

The invention provides an application of a rice OsCSN5 protein, relates to the technical field of crossing of biotechnology and plant breeding, and particularly provides an application of the rice OsCSN5 protein in a method for controlling the length of a main root of rice and the sensitivity of auxin of a rice root system. The invention further provides application of the rice OsCSN5 gene encoding the rice OsCSN5 protein in a method for controlling the length of a main root of rice and the sensitivity of auxin in a rice root system, the amino acid sequence of the rice OsCSN5 protein is shown as SEQ ID No.2, and the nucleotide sequence of the rice OsCSN5 gene is shown as SEQ ID No.1. The regulation and control effect of the OsCSN5 gene on the growth of the rice root system is analyzed for the first time, and a theoretical foundation is laid for further genetic improvement of rice root system molecules.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention relates to a gene for regulating and controlling the grain number of crops and application thereof. Specifically, the invention provides an application of GNR1 (Grain Number in Rice1) or an allele of the GNR1 in Rice1 in the aspect of improving the yield of rice by regulating and controlling the change of the grain number per spike of the rice. Also disclosed are methods of growing plants with increased grain number per ear, increased stem number and / or increased yield. The GNR1 gene has a wide application prospect in plant breeding.

The invention discloses an application of an OsATS3 gene in regulation and control of rice flooding stress tolerance. According to the invention, an OsATS3 gene is constructed to edit a knockout vector, an agrobacterium tumefaciens-mediated method is adopted to transform rice calluses, and a knockout strain of the OsATS3 gene is obtained through screening, and the knockout strain of the OsATS3 gene shows a phenotype that after seeds germinate under a waterflooding condition, seedling root systems are developed, seedling growth of overground parts of seedlings is faster, the growth speed of first leaves is faster, and the seedling rate is high; the method has great significance in solving the problems of slow seedling growth, inhibited radicle development, low seedling rate and the like in the rice direct seeding process, and has a wide application prospect in plant breeding.

The invention discloses application of a corn ZmTST1 gene in improvement of plant yield and stress resistance. The nucleotide sequence of the corn ZmTST1 gene is as shown in SEQ ID NO. 1; the stress resistance is tolerance to salt stress and drought stress. Through sequence analysis and functional verification, after the ZmTST1 gene is over-expressed, the growth of plants can be promoted, the yield of the plants can be increased, meanwhile, the drought tolerance and the salt tolerance of ZmTST1 expressed plants are obviously higher than those of wild plants, and it is proved that the ZmTST1 gene has a remarkable effect in the aspects of high yield and stress resistance. By identifying the ZmTST1 gene, plants with high stress resistance can be screened, the cultivation period of the plants can be shortened, and a new method and thought are provided for plant breeding in the future.

The invention relates to the field of plant breeding, in particular to application of a GhZAT10 gene in promoting low potassium stress tolerance of plants, a recombinant vector, a recombinant microorganism, a method for improving the low potassium stress tolerance of the plants and a plant breeding method. According to the application of the GhZAT10 gene in promoting the low potassium stress tolerance of the plant, the GhZAT10 in the plant is over-expressed to improve the low potassium stress tolerance of the plant and / or improve the potassium ion absorption capacity of the plant, and the nucleotide sequence of the GhZAT10 is shown as SEQ ID NO: 1. According to the application of the GhZAT10 gene in promoting the low-potassium stress resistance of the plant, the low-potassium stress resistance of the plant can be improved in a low-potassium environment in which K < + > is not added and a trace amount of K < + > is added in an external source, and the growth of the plant is remarkably promoted.

The present invention relates to the fields of biotechnology and plant breeding, specifically providing a method for fixing heterosis in rice using the DMP gene. To address the low fruit set rate and complex operation issues of existing apomictic reproduction systems, the present invention verifies for the first time the haploid-inducing function of the rice genes OsDMP8 and OsDMP13. Furthermore, the CRISPR / Cas9 technology is used to simultaneously knock out the OsPAIR1, OsREC8, OsOSD1, and OsDMP genes, constructing a "MiMe-OsDMP" four-gene editing system. This system provides new ideas for constructing apomictic reproduction systems in rice and offers a new solution for fixing heterosis in rice apomictic reproduction. This system can significantly improve the efficiency and quality of agricultural production, and has significant economic value and broad application prospects.

The invention discloses application of TaNOP2 protein and a coding gene thereof in wheat breeding. The invention also protects the application of the TaNOP2 protein or the TaNOP2 gene as an inhibition target in plant breeding. The target of the plant breeding is to cultivate plants with increased plant height and / or yield and / or tillering number and / or spike length and / or spike weight and / or grain number per spike and / or thousand grain weight and / or photosynthetic rate and / or grain length and / or grain width and / or grain perimeter and / or grain surface area. The invention provides a new thought and a potential target for wheat molecular breeding, can be used for cultivating high-yield new wheat germplasm, and has industrial popularization value.

The invention discloses a method for efficiently promoting slash pine somatic embryo maturation, and relates to the technical field of plant breeding. According to the method, the slash pine embryogenic callus is inoculated into a somatic embryo maturation culture medium containing sulfonatin and / or activated carbon, or PEG8000 and / or chitosan oligosaccharide for somatic embryo induction culture, so that early maturation of slash pine somatic embryos can be remarkably promoted. Results of the invention show that when the concentration of the sulfokine added in the culture medium is 0.5 mg / L, the total number of mature somatic embryos is as high as 109 / dish; when 0.5 mg / L of sulfonatin and 1 mg / L of activated carbon are added at the same time, the number of mature somatic embryos is up to 288 / dish; when 120g / L of PEG 8000 and 6mg / L of chitosan oligosaccharide are added at the same time, the number of mature somatic embryos is up to 123 / dish. In conclusion, the method disclosed by the invention can efficiently promote slash pine somatic embryo maturation, and is high in somatic embryo induction efficiency and strong in practicability.

The invention discloses a grape downy mildew resistance whole genome selective breeding method based on machine learning, which comprises the following steps: randomly dividing 132 samples into a training set and a test set according to a ratio of 4: 1, and respectively extracting first 1-100 thousand variation sites as gradient training data sets according to a whole genome association analysis result; and on the basis of 10 classic regression models and the dimensionality-reduced variation data set, machine learning training and evaluation are carried out on 105 training set samples, finally, the variation data set with the highest prediction accuracy and an optimal model are screened out, and phenotype prediction is carried out on 27 test set samples by using the optimal model. The invention relates to the technical field of plant breeding, and the grape downy mildew resistance whole-genome selective breeding method based on machine learning has remarkable advantages in resistance prediction and screening, breeding efficiency improvement and multi-character comprehensive improvement, breaks through the limitation of a traditional breeding method, and is suitable for large-scale popularization and application. An innovative solution is provided for green and sustainable development of the grape industry.

The invention discloses a SiERF109 gene for regulating drought resistance, salt tolerance and flavonoid accumulation of millet and application of the SiERF109 gene, and belongs to the technical field of plant breeding. According to the invention, a gene SiERF109 with a nucleotide sequence shown as SEQ ID NO.1 is separated and cloned from millet, and fluorescent quantitative expression analysis shows that SiERF109 participates in drought and salt stress response of millet. After the gene is over-expressed in foxtail millet, in drought and salt stress experiments, compared with a wild type strain, the survival rate and chlorophyll content of a strain over-expressed with the SiERF109 gene are remarkably increased, the flavonoid content is increased by 33.5%-39.5%, and active oxygen accumulation is remarkably reduced; it is shown that the SiERF109 gene and the protein coded by the SiERF109 gene can remarkably improve the drought resistance, the salt resistance and the flavonoid content of the plant.

The invention belongs to the technical field of plant breeding, and relates to a transgenic potato event ST2400643 and a detection method thereof. The invention provides a nucleic acid molecule for detecting a transgenic potato event ST2400643. The sequence of the nucleic acid molecule is shown as SEQ ID NO: 1-2 or a complementary sequence of the SEQ ID NO: 1-2. Or the sequence of the nucleic acid molecule is shown as SEQ ID NO: 3 or a complementary sequence thereof; the nucleic acid molecule is derived from a transgenic potato event ST2400643, and a potato plant containing the transgenic potato event ST2400643 has been preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC NO.46132. A potato plant comprising the transgenic potato event ST2400643 of the invention has the following advantages: the potato plant can tolerate imidazolinone herbicides; the potato yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

The invention discloses a wheat mutant protein with glyphosate resistance, a gene and application thereof, and belongs to the technical field of plant breeding. The invention discloses wheat mutant proteins GR1 and GR19 with glyphosate resistance, six mutation sites and three mutation sites exist on an ESPSP protein respectively, and amino acid sequences of the 128 to 308 sites and amino acid sequences of the 133 to 308 sites are shown as SEQ ID NO. 4 and SEQ ID NO. 5 respectively. Two mutants with glyphosate resistance and excellent agronomic traits are found in a wheat mutant group by taking glyphosate as a screening agent, the two mutants are named as common wheat ZM9-GR1 and common wheat ZM9-GR19, and the invention discloses application of the mutants in plant breeding. The invention also discloses a method for cultivating glyphosate-resistant wheat by using the mutant for backcross breeding and cross breeding. The invention has important application value in plant breeding and agricultural production.

The invention discloses a rice protein OsFBX388 related to plant disease resistance as well as a coding gene and application of the rice protein OsFBX388. The invention provides an application of OsFBX388 protein or a related biological material thereof in regulation and control of plant disease resistance, and also provides a substance for reducing the content or activity of the OsFBX388 protein, or a substance for reducing or inhibiting expression of a coding nucleic acid molecule of the OsFBX388 protein, and any one of the following applications: 1) improving the plant disease resistance; 2) cultivating plants with improved disease resistance; and 3) plant breeding. The OsFBX388 gene has the advantages that the OsFBX388 gene is down-regulated in expression, so that rice can spontaneously generate disease-like spots, the resistance to rice blast and bacterial leaf blight is enhanced, and the OsFBX388 gene has good application potential and application value in improvement of crop disease resistance.

An epigenetic regulatory factor ZmULT1 related to corn grain size regulation and heat stress response and the use thereof, which solve the technical problems of how to increase the grain size and / or heat resistance and / or lodging resistance of corn. Use of a substance for reducing the content of the protein ZmULT1 or a substance for inhibiting the expression of the encoding gene of the protein ZmULT1, which use is any one of the following: P1, the use in increasing the grain size of corn; P2, the use in improving the heat resistance of corn; P3, the use in increasing the lodging resistance of corn; and P4, the use in plant breeding. The homozygous mutant strain with knockout of the ZmULT1 gene has a reduced plant height and an increased grain size, and exhibits significant heat stress resistance, this indicates that the research on the gene can further enrich the use of epigenetics in crops and is of important significance for improving the grain size, heat resistance and lodging resistance of corn.