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Methods and compositions for breeding for preferred traits

A plant breeding and plant technology, applied in the direction of botany equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of high growth, large crop loss, expensive, etc.

Active Publication Date: 2010-09-01
MONSANTO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, high resource input is required to induce haploidization followed by diploidization
Diploidization represents a rate-limiting step as it is expensive and requires high labor input as well as plant material to generate sufficient breeding material
GLS in maize causes increased resource allocation to damaged leaf tissue, leading to increased risk of root and stem rot and ultimately greater crop loss (Ward et al. 1999; Saghai-Maroof et al. 1996 Theor.Appl.Genet .93:539-546)

Method used

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  • Methods and compositions for breeding for preferred traits
  • Methods and compositions for breeding for preferred traits
  • Methods and compositions for breeding for preferred traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0242] Example 1: Genotyping of GLS responses

[0243] To detect QTLs associated with GLS resistance, plants were genotyped to determine GLS response. The rating scale below was used for phenotypic assessment of GLS and was used in all studies. The percentage of leaf area infected was used to rate plants on a scale from 1 (very high resistant) to 9 (susceptible). Disease resistance was assessed visually after pollination. Infection can be natural, or from artificial inoculation in experiments.

[0244] Table 1. Description of rating scales used for GLS phenotyping. ILA = infected leaf area.

[0245] illustrate

[0246] illustrate

Embodiment 2

[0247] Example 2: GLS Resistance Mapping Study 1

[0248] To examine the correlation between SNP markers and GLS resistance in maize, analytical data from many studies were combined. An association study was performed to assess whether there was a significant association between one or more marker genotypes and GLS resistance in one or more breeding crosses. The mapping study synthesized data from 176 mapping groups. The number of individuals in each population ranged from 95 to 276. The segregating populations were the following generations: F2, BC1F2, BC1 and DH. The number of SNP markers used for genotyping ranged from 55 to 158. Individuals were phenotyped for traits, including GLS resistance. A total of 2499 SNP markers on chromosomes 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 were identified for association with GLS resistance. The provided SNP markers can be used to monitor the introgression of GLS resistance into breeding populations. SNP markers, significance levels and f...

Embodiment 3

[0249] Example 3: GLS Resistance Mapping Study 2

[0250] An association study was performed to assess whether there was a significant association between one or more marker genotypes and GLS resistance in one or more breeding crosses. In the association study, 769 F2s from the CV128 / CV162 population were screened using 117 markers. Associations between a total of 53 SNP markers and GLS resistance were determined on chromosomes 1, 2, 3, 4, 5, 6 and 8. The provided SNP markers can be used to monitor the introgression of GLS resistance into breeding populations. SNP markers, significance levels and favorable alleles associated with GLS resistance in figure 1 report in.

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Abstract

The present invention relates to the field of plant breeding. More specifically, the present invention includes a method of using haploid plants for genetic mapping of traits such as disease resistance. Further, the invention includes a method for breeding corn plants containing quantitative trait loci (QTL) that are associated with resistance to Gray Leaf Spot (GLS), a fungal disease associated with Cercospora spp. The invention further includes a method for breeding corn plants containing QTL that are associated with Goss' Wilt, a bacterial disease associated with Clavibacter michiganense spp.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Patent Application 60 / 966,706, filed August 29, 2007, the entire disclosure of which is hereby incorporated by reference. [0003] Introduction of Sequence Listing [0004] Submit here electronically the Sequence Listing containing 2,432,383 bytes (on Microsoft Windows In the file named "46_25_54886_0000_WO.txt" in Statistics), and all of them are imported here. field of invention [0005] The invention belongs to the field of plant breeding. More specifically, the invention includes a method for genetic mapping of traits such as disease resistance using haploid plants. Furthermore, the present invention includes a method of breeding maize plants containing a quantitative trait locus (QTL) associated with resistance to gray leaf spot (GLS), a fungus related to the genus Cercospora spp. sick. The invention further includes a method of growing corn plants containi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H5/10
CPCC12Q1/6895A01H5/10A01H1/04C12Q2600/13C12Q2600/156C12Q2600/172A01H6/4684A01H1/045C12N15/8281C12N15/8282
Inventor D·巴特鲁伊勒S·伊瑟英格顿T·J·弗雷D·博克尔曼M·R·克恩斯T·卡尔森R·韦里奇K·格罗特贾红武G·波扎K·库克S·沃克G·霍兰L·彼得斯
Owner MONSANTO TECH LLC
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