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a Streptomyces

A technology of streptomyces and bacterial strains, applied in the direction of bacteria, fungicides, biocides, etc., can solve the problems of low control efficiency and unsatisfactory antibacterial activity

Active Publication Date: 2021-05-28
JILIN CITY ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of biological control, there are mainly problems such as unsatisfactory antibacterial activity and low control efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 111

[0057] Example 1.1.1 strain screening

[0058] In August 2018, twelve soil samples were randomly collected from the larch forest of Hengshan Conservation Station in Changbai Mountain National Nature Reserve, Jilin Province, Maanshan spruce plantation in Chixi Conservation Station, and Qianchuan Forest Farm in Fusong County. Collection method: first use a small shovel to remove the topsoil, take about 200g of soil at a depth of 5-10cm, put them into plastic bags respectively, and put them into labels with the collection place and collection time for the isolation of streptomyces. If it cannot be separated immediately, dry the soil sample in the shade and store it in a cool place.

[0059] Isolation and purification of biocontrol Streptomyces were carried out by dilution separation method. Add 10g of each weighed soil sample to 100ml of sterile water, shake for 30 minutes, draw 1ml of the suspension, and dilute it with sterile water gradient, from 10 -3 (i.e. diluted 10 3 tim...

Embodiment 112

[0060] The mensuration of embodiment 1.1.2 bacterial strain antibacterial activity

[0061] Taking D. gregaria as the target bacteria, the in vivo screening of antagonistic Streptomyces was carried out by plate confrontation culture method. Make a 5mm bacterial cake of the target bacteria and inoculate it on the center of a potato dextrose agar plate (PDA plate) (plate diameter 90mm), pick up the actinomycetes for 72 hours from the inoculation loop at a distance of 1.75cm from the upper and lower sides of the bacterial cake, draw lines in parallel, and keep at 28°C The width of the inhibition zone between actinomycetes and pathogenic fungi was measured after constant temperature culture for 72 hours, and each treatment was repeated 3 times. The strains for the fermentation test were determined according to the size and stability of the inhibition zone.

Embodiment 113

[0062] Embodiment 1.1.3 Determination of antibacterial spectrum of biological control bacterial strain living body and fermented liquid

[0063] The antibacterial spectrum of strain HS1 in vivo was determined by the plate confrontation culture method. There were 13 kinds of pathogenic bacteria in the antibacterial spectrum. The width of the inhibition zone between the biocontrol strain and each pathogenic fungus was measured, and each treatment was repeated 3 times.

[0064] Routine production of fermentation medium, formula: by mass percentage, starch 7.0%, peanut cake powder 3.3%, (NH 4 ) 2 SO 4 0.4%, CaCO 3 0.4%, NaCl 0.4%, distilled water 1000ml, pH7.0. Select the biocontrol strain HS1 with strong antibacterial activity and good stability and transfer it to the fermentation culture medium. The loading volume is 40ml of the fermentation broth in the triangular bottle of 250ml, and the inoculation amount is 5 bacterial blocks. / min) for 5 days, the fermentation broth was...

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Abstract

The invention relates to a streptomyces, and the preservation number of the strain is CGMCC No.18657. The streptomyces is an antagonistic streptomyces which can effectively inhibit various pathogenic bacteria such as poplar canker bacterium. The streptomyces described in the invention has the advantages of wide antibacterial spectrum, obvious antibacterial effect, stable effect, low toxicity and environmental protection.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a streptomycete. Background technique [0002] Poplar canker is a major biological disaster of poplar plantations in my country, and the damage is becoming more and more serious. At present, in addition to improving young tree varieties and enhancing the disease resistance of trees, the prevention and control of poplar canker mainly adopts chemical control measures. This method is low in cost and quick in effect, and has been widely used for a long time. The disadvantage is that the long-term use of chemical pesticides will enhance the drug resistance of pathogenic bacteria, resulting in a large amount of pesticide residues and polluting the environment. Biological control has strong specificity, persistence and effectiveness in inhibiting pathogenic bacteria, and can enhance the resistance of host plants. It is the main research direction of forest disease control at present. Pop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00C12R1/465
CPCA01N63/28C12N1/20
Inventor 李立梅陈越渠邹建军陈思羽燕红马琼芳周勇刘晓林刘庆珍左彤彤沈佳龙于海媛张晓光朱春玉郑方亮丁芮涵
Owner JILIN CITY ACADEMY OF FORESTRY
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