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551 results about "Streptomyces species" patented technology

Compound probiotics preparation for biological organic fertilizers and preparation method thereof

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.
Owner:周剑平 +1

Lipolytic enzyme: uses thereof in the food industry

The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to an acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ ID NOs: 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID NO: 3, 6, 9, 13, 15 or 17 or a nucleotide sequence which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Streptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID NO: 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence show in SEQ ID NO: 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID NO: 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID NO: 3.
Owner:DUPONT NUTRITION BIOSCIENCES APS

Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof

The invention provides a microorganism compound bactericide for controlling cucumber fusarium wilt and a preparation method thereof. The microorganism compound bactericide for controlling cucumber fusarium wilt is characterized by consisting of 10 to 15 parts by weight of rhodopseudanonas palustris, 30 to 40 parts by weight of bacillus subtilis, 15 to 18 parts by weight of streptomyces, 20 to 23 parts by weight of lactobacillu plantarum and 10 to 15 parts by weight of saccharomyces cerevisiae. The preparation method is characterized by comprising the following steps of: screening the rhodopseudanonas palustris, the bacillus subtilis and the streptomyces, and respectively inoculating and culturing in a flask in which nutrient agar culture media are put; mixing obtained strains with the lactobacillu plantarum and the saccharomyces cerevisiae; and inoculating mixed lawn in a liquid culture dish, and fermenting in a fermentation tank to obtain the microorganism compound bactericide. In the invention, soil ecological environment is repaired and improved under the condition of inhibiting the reproduction of fusarium wilt pathogenic bacteria and controlling pathogenetic scale and degree,meanwhile, cucumber growth and yield are improved.
Owner:SHANGHAI CHUANGBO ECOLOGICAL ENG

Domestic garbage and sludge decomposing bacterium agent and application thereof and prepared decomposing matrix

The invention discloses a rural domestic garbage and sludge decomposing bacterium agent and a using method as well as a decomposing matrix prepared by using the bacterium agent and a specific process through fermentation. The bacterium agent is compounded by 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum, 0.5-2 parts of aspergillus nige and 0.5-2 parts of bacillus mucilaginosus in parts by weight; the effective viable count of the bacterium agent is 5*10<8>/g; the bacterium agent can be used for decomposing garbage and sludge, deodorizing, resisting fusarium wilt of cucumber and increasing the yield when being applied to crops; the fermentation period of the treated garbage and sludge is 20 days when the bacterium agent is inoculated and 30 days when the bacterium agent is not used. The decomposing matrix is prepared by adding 1 kilogram of bacterium agent into every 5 square mixture through the specific process and fermentation; the mixture is formed by mixing 40-60% of domestic garbage, 20-40% of sludge and 5-15% of corn straws in percentage by weight; when the matrix is applied in cucumber cultivation, the yield is increased by 11% per mu, the income is increased by 859 yuan, and 200mL of pesticide is saved.
Owner:天津市农业科学院

Biological organic fertilizer for preventing and treating root-knot nematode and preparation method and application of biological organic fertilizer

The invention provides a biological organic fertilizer for preventing and treating root-knot nematode and a preparation method and application of the biological organic fertilizer. The biological organic fertilizer comprises 5-30 parts of a composite bacterial agent and 70-95 parts of a carrier, wherein the composite bacterial agent contains bacillus subtilis, paecilomyces lilacinus, pochonia chlamydospora and streptomycete, and the carrier is made from chicken manure, mushroom residues, and rape seed cake through microorganism compost fermentation. The composite bacterial agent has the effectof preventing and treating root-knot nematode in soil. The carrier is abundant in organic components and inorganic components, which can improve soil, increase the nutritive components in soil, accelerate the effective colonization of the composite bacterial agent in soil, and improve the capability of controlling treating root-knot nematode in soil of the composite bacterial agent. The providedbiological organic fertilizer for preventing and treating root-knot nematode is powder, can be used as a base fertilizer, is harmless to human and animals, is good in preventing and treating effect, and can obviously improve the quality and yield of crops.
Owner:HUNAN TAIGU BIOTECH

Composite biological seed coating agent used for controlling vegetable soil-borne diseases and preparation and application methods thereof

The invention discloses a composite biological seed coating agent used for controlling vegetable soil-borne diseases and preparation and application methods thereof; the composite biological seed coating agent comprises bacillus subtilis and streptomyces rochei, also contains water as a carrier medium, sodium carboxymethyl cellulose as an adhesive, sodium lignin sulfonate as a dispersing agent, chitosan and polyvinyl alcohol as a film forming agent, sodium benzoate as a preservative and rhodamine as a colorant; the preparation method is as follows: (1) respectively preparing bacillus subtilis and streptomyces rochei fermentation liquids; (2) stirring and mixing the bacillus subtilis and streptomyces rochei fermentation liquids in a ratio of 2:1; (3) proportion ally adding the sodium carboxymethyl cellulose, the sodium lignin sulfonate, the chitosan, the polyvinyl alcohol, the sodium benzoate and the rhodamine, and finally complementing the margin with the water.; and the application method is as follows: coating, root dipping and root irrigation. The composite biological seed coating agent is obvious in disease resistance effect and long in effective period, has the effects of saving seeds, strengthening seedlings and the like, and is free of environmental pollution.
Owner:JIANGSU LIXIAHE REGION AGRI RES INST

Pig-raising fermentation bacterial agent for biological and environment-friendly fermentation bed and preparation method thereof

InactiveCN102577985AAntioxidantHas anti-tumor functionFungiBacteriaBiotechnologyPig breeding
The invention discloses a pig-raising fermentation bacterial agent for a biological and environment-friendly fermentation bed and a preparation method thereof. The preparation method comprises the following steps of: firstly selecting seven strains of bacteria, namely Morchella sp. CGMCC (China General Microbiological Culture Collection Center) NO.5.496, Bacillus stearothermophilus CGMCC NO.1.1923, Bacillus stearothermophilus CGMCC NO.1.1866, Bifidobacterium thermoacidophilum CGMCC NO.1.2282, Bacillus subtilis subsp. subtilis CGMCC NO.1.108, Streptomyces anandii CGMCC NO.4.1314, Saccharomycescerevisiae CGMCC NO.2.2081 as production and fermentation parent agents, wherein the viable count of each production and fermentation parent agent is not less than 108/ml; and then performing freeze-drying on the seven strains of the production and fermentation bacterial agents, fully and uniformly mixing according to the weight of each powder freeze-dried strain, and then performing vacuum packaging to obtain a complex bacterial agent. By using the fermentation bed manufactured by the fermentation bacterial agent, the odor of a pigpen can be obviously reduced, the breeding opportunities of flies and mosquitoes can be reduced, the immunity of pigs can be improved, the healthy growth of the pigs can be protected, and the fermentation bed simultaneously has the characteristics of simplicityin practical operation and simplicity in manufacturing of the fermentation bed.
Owner:WEIWU JINLVYUAN AGRI TECH DEV

Method for producing microbial fertilizer by utilizing kitchen waste

The invention provides a method for producing a microbial fertilizer by using kitchen waste. The microbial fertilizer contains one selected from the group consisting of Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus megatherium, Brevibacillus laterosporus, Bacillus thuringiensis, Bacillus subtilis and Streptomyces microflavus or mixed bacteria of two or more selected from the group. The method for producing the microbial fertilizer comprises the following steps: removing foreign matters in the kitchen waste, heating the kitchen waste, taking out grease, crushing the rest kitchen waste, adding enzyme for enzymolysis, putting the kitchen waste having undergone enzymolysis into a fermentation tank, performing sterilizing, inoculating one or more culturedstrains, performing fermenting with a pure bacterium or mixed bacteria to obtain a single-bacterium liquid microbial fertilizer or a composite-bacteria liquid microbial fertilizer, and adsorbing the liquid bacterial fertilizer with a carrier to obtain the solid microbial fertilizer. The microbial fertilizer is produced by utilizing the kitchen waste, so production cost is remarkably reduced, and the pollution of the kitchen waste to the environment can be effectively reduced.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Special complex microbial agent for harmless treatment of livestock and poultry excrements, and preparation method

The invention discloses a special complex microbial agent for harmless treatment of livestock and poultry excrements. The special complex microbial agent is prepared from the following raw materials in parts by weight: 0.02-0.04 part of bacillus mucilaginosus, 0.01-0.03 part of bacillus licheniformis, 0.01-0.03 part of streptomyces jingyangensis whey powder, 0.01-0.02 part of azotobacter vinelandii, 0.04-0.08 part of aspergillus spore powder, 0.01-0.03 part of streptococcus thermophilus, 0.01-0.02 part of enterococcus faecalis, 0.02-0.04 part of antimicrobial peptide thallus, 80.0-120.0 parts of dried silkworm dropping, 2.0-6.0 parts of peanut cakes, 2.0-3.0 parts of bran, 7.0-8.0 parts of starch, 3.0-3.5 parts of molasses, 2.0-3.0 parts of monocalcium phosphate, and 1.0-1.4 parts of urea. Used for harmless treatment of livestock and poultry excrements, the special complex microbial agent has the advantages that the fermentation time is short, the quality of a fertilizer is high, the active rate of microorganisms in soil can be activated, the temperature permeability of air in soil can be increased, and the special complex microbial agent has an effect of preventing and controlling various fungal diseases and bacterial diseases, as well as effects of increasing the yield and improving the quality while providing complete nutrients for the crop, thus having better economic benefits, social benefits and ecological benefits.
Owner:GUANGXI SANBAO AGRI SCI & TECH
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