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575 results about "Streptomyces species" patented technology
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Saline-alkali soil improving agent and its preparation method
ActiveCN102344814AImprove the environmentEnvironmental regulationAgriculture tools and machinesOther chemical processesAlkali soilFermentation starter
The invention relates to a saline-alkali soil improving agent and its preparation method. The preparation method comprises the following steps: mixing Rhodopseudomonas palustris, Pichia membranefaciens Hansen bacterium, lactobacillus casei, Staestomyces microflavus and seaweed slurry, organically combining a plurality of beneficial microorganisms and seaweed through multi-time fermentation, culturing in a compound mode to obtain a novel microorganism agent-seaweed bio-fungus. adding a proper amount of humic acid, organic matter, active zeolite powder and molasses, fermenting and drying the fermented product. The saline-alkali soil improving agent uses a plurality of microorganisms and employs strong effects of a unique ionic adsorbent, an organic neutralization balancing agent, an ion-exchange agent and a beneficial bacteria conversion starter to achieve the purposes of improving the soil environment, regulating acid-base balance, reducing the salt content, effectively raising the soil fertility and permeability, loosening the soil, eliminating the soil hardening, degrading the harmful substance and promoting the accumulation and conversion of the organic substance.
Owner:日照益康有机农业科技发展有限公司
Compound probiotics preparation for biological organic fertilizers and preparation method thereof
InactiveCN102229900APromote growthImprove firmnessBio-organic fraction processingFungiAureobasidium sp.Streptomyces variabilis
The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.
Owner:周剑平 +1
Agricultural microbial fertilizer and preparation method and application thereof
InactiveCN106906162ASolve the long-standing problem of hardeningEffective controlFungiBacteriaFertilizerNutrient
The invention relates to an agricultural microbial fertilizer and a preparation method and application thereof. The agricultural microbial fertilizer comprises a medium and thalli. The thalli comprise bacillus subtilis, Bacillus megaterium, Bacillus mucilaginosus, Bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, Bacillus mucilaginosus, Streptomyces jingyangensis, azotobacter vinelandii, bacillus coagulans, Aspergillus oryzae and paecilomyces lilacinus. The agricultural microbial fertilizer provided by the invention can improve soil, can effectively control part of plant diseases and insect pests and can supply nutrients to soil to effectively solve a long-term problem of soil hardening. In addition, the agricultural microbial fertilizer can dissolve phosphorus and potassium so as to affectively release phosphorus, potassium, etc. existing in the form of a chelate in the soil.
Owner:郑州绿动源生物科技有限公司
Lipolytic enzyme: uses thereof in the food industry
Owner:DUPONT NUTRITION BIOSCIENCES APS
Novel strain of streptomyces fradiae and application thereof
ActiveCN102329754ASignificant disease resistanceSignificant nitrogen fixation and phosphorus releaseBio-organic fraction processingBacteriaMicroorganismMicrobiology
The invention discloses a novel strain CLIMAX-VSF of streptomyces fradiae. The preservation number of the novel strain is CGMCC No. 5133; the preservation date is August 12, 2011; and the preservation department is China General Microbiological Culture Collection Center. The invention further discloses a microbial inoculum containing the novel strain. The novel strain CLIMAX-VSF of the streptomyces fradiae, which is provided by the invention, has quite obvious performances of disease resistance, nitrogen fixation, phosphate solubilization and somatotropin secretion. By using the strain, the germination rate and the growth speed in a seedling stage can be obviously improved under the condition that nutrients are not added to soil, and furthermore, diseases in the seedling stage and a growth period are obviously inhibited. On the aspect that the strain is used for the mixed preparation and the synergy of a fertilizer, the strain shows to have the favorable effect of activating soil nutrients and the performance of resisting a plurality of cultivation obstacles.
Owner:BEIJING JINGGENG TIANXIA AGRI TECH
Compound microbial bacterial preparation for efficiently degrading feces and preparation method and application of compound microbial bacterial preparation
InactiveCN102690769AShorten fermentation timeEfficient killingFungiBio-organic fraction processingMicroorganismFeces
The invention relates to a compound microbial bacterial preparation for efficiently degrading feces and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, bacillus megaterium, geobacillus stearothermophilus, aspergillus oryzae, trichoderma longibrachiatum, sporotrichum thermophile and streptomycesmicroflavus through solid fermentation. The compound microbial bacterial preparation can shorten the fermentation time of feces, and effectively kill larva, eggs and pathogens in the feces, and further improves the content of organic matters, total nitrogen, total phosphorus, and total potassium in the fermented materials.
Owner:DEZHOU YUANHE AGRI TECH DEVCO
High-efficiency hardening-resisting continuous-cropping-resisting soil-modifying biological agent
InactiveCN102491853AWell mixedReduce pollutionFertilizer mixturesContinuous croppingBacillus licheniformis
The invention relates to a high-efficiency hardening-resisting continuous-cropping-resisting soil-modifying biological agent. The biological agent is composed of: 20% of trichoderma, 20% of streptomyces microflavus, 30% of starch, 10% of grass peat, 8.15% of a siliceous fertilizer, 6% of humic acid, 5% of urea, 0.2% of biological strains (including 1 / 3 of bacillus subtilis, 1 / 3 of bacillus cereus, and 1 / 3 of bacillus licheniformis), 0.1% of vitamin C, 0.5% of compound sodium nitrophenolate, and 0.05% of DA-6. With the soil-modifying biological agent provided by the invention, defects of existing fertilizers are overcome, the pollution to the environment is reduced, soil is activated, soil hardening and continuous-cropping are overcome, and substantial influences are provided for healthy growth and bumper harvest of crops.
Owner:ZHENGZHOU YONGFENG BIOLOGICAL FERTILIZER IND CO LTD
Microbial deodorizing microbial inoculum as well as preparation method and application thereof
InactiveCN103540539AInhibition of growth and reproductionReduced odor release intensityFungiMicroorganism based processesLichenLitter
The invention discloses a microbial deodorizing microbial inoculum for public toilets, livestock and poultry farms and refuse collection points. The microbial deodorizing microbial inoculum contains pichia jadinii with the preservation number of CGMCCNO.5982, issatchenkia orientalis with the preservation number of CGMCCNO.6496, lactobacillus sp. with the preservation number of CGMCCNO.6495, streptococcus thermophilus with the preservation number of CGMCCNO.5985, bacillus licheniformis with the preservation number of CICCNO.20085 and streptomyces microflavus var. lactosus with the preservation number of CGMCCNO.4891. The invention also discloses a preparation method of the microbial deodorizing microbial inoculum in both liquid and solid states, and application of the microbial deodorizing microbial inoculum.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof
InactiveCN102010825AGuaranteed CompatibilityTake root quicklyBiocideFungiBiotechnologyEcological environment
The invention provides a microorganism compound bactericide for controlling cucumber fusarium wilt and a preparation method thereof. The microorganism compound bactericide for controlling cucumber fusarium wilt is characterized by consisting of 10 to 15 parts by weight of rhodopseudanonas palustris, 30 to 40 parts by weight of bacillus subtilis, 15 to 18 parts by weight of streptomyces, 20 to 23 parts by weight of lactobacillu plantarum and 10 to 15 parts by weight of saccharomyces cerevisiae. The preparation method is characterized by comprising the following steps of: screening the rhodopseudanonas palustris, the bacillus subtilis and the streptomyces, and respectively inoculating and culturing in a flask in which nutrient agar culture media are put; mixing obtained strains with the lactobacillu plantarum and the saccharomyces cerevisiae; and inoculating mixed lawn in a liquid culture dish, and fermenting in a fermentation tank to obtain the microorganism compound bactericide. In the invention, soil ecological environment is repaired and improved under the condition of inhibiting the reproduction of fusarium wilt pathogenic bacteria and controlling pathogenetic scale and degree,meanwhile, cucumber growth and yield are improved.
Owner:SHANGHAI CHUANGBO ECOLOGICAL ENG
Streptomyces microflavus
InactiveCN101864378AStrong antagonistic effectBroad spectrum antibacterialBiocideBacteriaBacteroidesBacterial strain
The invention discloses a streptomyces microflavus. The streptomyces microflavus is the streptomyces microflavus NMG2-4-8, and the preservation number thereof is CGMCG No.3442. The streptomyces microflavus is separated and screened from alfalfa healthy tree rhizosphere soil for the first time in the invention, and experiments prove that the streptomyces microflavus has remarkable antagonistic action for 18 pathogenic fungi and 9 bacteria and broad antibacterial spectrum. The streptomyces microflavus has strong inhabiting action for crop pathogenic bacteria, such as alfalfa downy mildew and the like, shows a good disease prevention effect for the alfalfa downy mildew, powdery mildew, brown spots, stemphylium leaf spot disease, root rot disease and the like, and indicates that the bacterial strain has broad application prospect in the biological prevention and cure fields of alfalfa diseases.
Owner:GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
Streptomyces strain and application thereof
ActiveCN101525587APromote growthImprove the effect of increasing productionBiocideBacteriaDiseaseSocial benefits
The invention discloses a Streptomyces sp. strain and the application thereof. The strain provided by the invention is Streptomyces sp. YL04 CGMCC No.2884. The strain YL04 provided by the invention with ideal biocontrol effect and development value can be popularized in large-area fields and mainly solves the problem that increasingly serious damping-off and blight diseases of crop seedlings like vegetables and melons cause serious output reduction or reduced product quality. The invention has significant scientific meaning and application value in enriching strain resources for biocontrol of our country and provides effective measures for achieving high-output and high-quality modern agriculture. The invention meets the national standards of agricultural goods production safety and the need of constructing a non-polluted, green and organic agricultural production base and has certain economic and social benefits.
Owner:CHINA AGRI UNIV
Thermostable carboxylesterase gene, coding protein and application thereof
The invention discloses a thermostable carboxylesterase gene, coding protein and application thereof, the esterase has amino acid sequence shown in SEQ ID NO:1. Compared with the prior art, the esterase containing EstW is thermostable esterase obtained from streptomycete in known mesophilic bacteria, can be widely applied to production of non-steroidal anti-inflammatory drugs or ferulic acid.
Owner:ANHUI NORMAL UNIV
Environmentally-friendly microorganism preparation for eliminating house-fitment pollution and preparation method thereof
InactiveCN101974430AGood removal effectGrowth inhibitionFungiBacteriaLactobacillus acidophilusPseudomonas veronii
The invention discloses an environmentally-friendly composite microorganism preparation for eliminating house-fitment pollution. The composite microorganism preparation comprises the following bacterial components in percentage by weight: 15 to 35 percent of lactobacillus acidophilus, 15 to 35 percent of candida utilis, 5 to 25 percent of bacillus licheniformis, 10 to 25 percent of streptomyces albulus and 15 to 30 percent of rhodopseudomonas palustris and is obtained by compositely culturing the bacterial components by a fermentation process which combines anaerobic fermentation with aerobic fermentation.
Owner:北京意科乐生态科技有限公司
Microbial preparation for inhibiting root-knot nematode and preparation method and application thereof
ActiveCN103039534AImprove securityFew cropping diseasesBiocidePlant growth regulatorsBiotechnologyMetabolite
The invention belongs to the field of biological prevention and control on crop insect damage and provides a microbial preparation for inhibiting root-knot nematode. The preparation is formed by mixing bacterial liquids of streptomyces avermitilis, paecilomyces lilacinus, verticillium chlamydosporium and Bacillus sphaericus and glycerol. The invention also provides a production method and an application effect of the microbial preparation for inhibiting the root-knot nematode. The microbial preparation comprises viable bacteria of which the number is greater than 2,500 millions per milliliter, wherein the viable bacteria comprise the streptomyces avermitilis, the paecilomyces lilacinus, the verticillium chlamydosporium and the Bacillus sphaericus. After the microbial preparation is prepared into soil, eggs of the root-knot nematode are killed by metabolites generated in the continuous growth and reproduction activity process of the viable bacteria, so that a granular structure of the soil is improved, the utilization rate of a fertilizer is improved and an effect of increasing both production and income is achieved.
Owner:河北衡晓生物科技有限公司
Streptomyces hygroscopicus and use thereof
ActiveCN101486976AIncrease production capacityImprove fermentation effectBacteriaMicroorganism based processesStreptomyces hygroscopicusMicrobiology
The invention discloses a streptomyces hygroscopicus and applications thereof. The streptomyces hygroscopicus strain is preserved in China Center for Type Culture Collection with CCTCC NO. of M 207200 and the preservation date of December 14th, 2007. The strain with CCTCC NO. of M 207200 is a currently-separated natural strain with high sirolimus yield and excellent fermentation performance. The fermentation yield of the sirolimus-producing strain can reach over 600ug / ml, has relatively less fermentation byproducts, lowers post extraction difficulty, can reach the product yield of over 30 percent, and is applicable to industrial production.
Owner:HANGZHOU HUADONG MEDICINE GRP PHARMA RES INST +1
Streptomyces atratus and application of cyclic peptide compounds with same to preparing mycobacterium tuberculosis resistant medicines
ActiveCN106279370AGood inhibitory effectAntibacterial agentsBacteriaCyclic peptideAntituberculous drugs
The invention discloses streptomyces atratus and application of cyclic peptide compounds with the same to preparing mycobacterium tuberculosis resistant medicines. A structural formula of the cyclic peptide compounds is shown. A preservation number of the streptomyces atratus SCSIO Zh16 is CGMCC No.12198. The streptomyces atratus and the application have the advantages that the six cyclic peptide compounds are obtained from fermentation cultivation substances of the streptomyces atratus SCSIO Zh16 by means of separation, the cyclic peptide compound 6 is high in mycobacterium tuberculosis resistant activity, obvious effects of inhibiting mycobacterium tuberculosis can be realized by the cyclic peptide compound, accordingly, the cyclic peptide compounds can be used for preparing anti-tuberculosis medicines and can be used for treating tuberculosis, alternative compounds can be provided for developing novel anti-tuberculosis medicines, and the streptomyces atratus and the application have important significance on developing marine medicinal materials in China.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1
Process for producing tomatine using bacteria fermentation
InactiveCN101085989AShorten the fermentation cycleSimple fermentation systemBacteriaMicroorganism based processesLycopeneTomatine
The invention provides a method for preparing lycopene by using bacteria fermentation. The employed bacteria is Streptomyces rimosus sub.rimosus. Compared with current normal method, the invention is characterized by short fermentation period, simple system, low cost, high product purity, and suitability for industrial production.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Method for producing polypeptide antibiotics nosiheptide through fermentation
The invention discloses a method for producing polypeptide antibiotics nosiheptide through fermentation. The method comprises the following steps of: inoculating streptomyces actuosus to a slant culture medium for carrying out slant culture; inoculating an obtained slant with spores to a seed bottle to culture a seed solution; inoculating the seed solution to a fermentation culture medium for carrying out fermentation culture according to an inoculation quantity of 10 wt%; and further treating the fermented fermentation liquor to obtain nosiheptide crystals. The nosiheptide is produced by adopting a fermentation method. The method is simple and easy and is low in production cost. The polypeptide antibiotics nosiheptide produced by adopting the method can be used as a safe, high-efficiency and environmental-friendly novel feed additive.
Owner:江西兴鼎科技有限公司
Biocontrol actinomycete strain for preventing and controlling tobacco bacterial wilt and application thereof
The invention discloses a biocontrol actinomycete strain for preventing and controlling tobacco bacterial wilt and application method thereof and belongs to the technical field of micro-organisms. The strain is streptomyces K4, is collected in China General Microbiological Culture Collection Center on March 24, 2014 and has the collection number of CGMCC No. 8949. The strain generates active substances to inhibit the growth of tobacco bacterial wilt and exhibits better prevention and control effects on the tobacco bacterial wilt. The biocontrol actinomycete strain is used for preparing a biocontrol microbial agent and the biocontrol microbial agent can be applied together with rooting water when tobacco seedlings are transplanted; or the biocontrol microbial agent is irrigated into the roots at 4-5 days after the tobacco seedlings are transplanted. The microbial agent can be applied as a bacterial fertilizer prepared by mixing the microbial agent and an organic fertilizer. The strain K4 is obtained from tobacco field rhizosphere soil and is ecologically and harmoniously compatible with soil so that the strain K4 has good application prospects in the biological control of tobacco bacterial wilt.
Owner:INST OF PLANT PROTECTION FAAS
Biocontrol bacterium and application of biocontrol bacterium to preventing and controlling cotton blight and cotton greensickness
The invention discloses Streptomyces rochei 42561 which is preserved in the general microorganism center of the China Committee for Culture Collection of Microorganisms (CCCCM), with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.6556. The bacteriostatic and prophylactic actions of the biocontrol bacterium 42561 are comprehensively tested according to a cup-plate method, a mycelial growth rate inhibition method, a spore germination inhibition method, greenhouse pot culture and field tests; and the testing results show that the strain has a remarkable prevention effect on cotton blight and cotton greensickness, can be used as an effective biological pesticide preparation agent and has excellent application and development prospects.
Owner:TARIM UNIV
Production of tacrolimus (fk-506) using new streptomyces species
The invention relates to a new microorganism Streptomyces glaucescens MTCC 5115 and a process for producing the immunosuppressant agent, tacrolimus (FK-506) utilizing the new microorganism Streptomyces glaucescens MTCC 5115. The invention also relates to pharmaceutical compositions that include the tacrolimus (FK-506) and use of said compositions for treatment or prevention of the transplantation rejection of organs or tissues such as heart, kidney, liver, medulla ossium, skin, etc.
Owner:RANBAXY LAB LTD
Novel culture medium for producing monensin by fermenting streptomyces cinnamonensis and culture method
InactiveCN103937848AGood fermentation processGood control parametersMicroorganism based processesFermentationBiotechnologyChinese cinnamon
The invention relates to a novel culture medium for producing monensin by fermenting streptomyces cinnamonensis and a culture method. The fermentation unit of the monensin can be increased, the fermentation period can be shortened and the fermentation cost can be reduced by use of the formula of the culture medium and a fermentation control process, and moreover, the source of raw and auxiliary materials is avoided from environmental influence to the utmost extent, the sufficient supply of the raw and auxiliary materials is guaranteed, and then stable high-efficiency production of the monensin is realized.
Owner:宁夏泰瑞制药股份有限公司
Streptomycete for producing volatile antibacterial substances and application thereof
ActiveCN108977382AGrowth inhibitionReduce severityBiocideBacteriaLaboratory culturePratylenchus zeae
The invention provides streptomycete for producing volatile antibacterial substances and application thereof. The streptomycete is named as Streptomycesmicionensis TF78; the streptomycete was collected to Guangdong Microbiological Culture Collection Center on October 25th, 2017, and the collection number is GDMCC NO:60254. The streptomycete has the inhibiting functions on the pathogenic bacteria of cucumber blight, tomato gray mold, teak canker, banana fungal stem rot, rice blast fungi, mango phomopsis leaf spot, mango pestalotiopsis leaf spot, mango botryosphaeria branch blight, mango anthracnose and the like.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
Domestic garbage and sludge decomposing bacterium agent and application thereof and prepared decomposing matrix
ActiveCN103966149AImprove qualityPromote repairPlant growth regulatorsBio-organic fraction processingTrichoderma longibrachiatumAspergillus niger
The invention discloses a rural domestic garbage and sludge decomposing bacterium agent and a using method as well as a decomposing matrix prepared by using the bacterium agent and a specific process through fermentation. The bacterium agent is compounded by 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum, 0.5-2 parts of aspergillus nige and 0.5-2 parts of bacillus mucilaginosus in parts by weight; the effective viable count of the bacterium agent is 5*10<8> / g; the bacterium agent can be used for decomposing garbage and sludge, deodorizing, resisting fusarium wilt of cucumber and increasing the yield when being applied to crops; the fermentation period of the treated garbage and sludge is 20 days when the bacterium agent is inoculated and 30 days when the bacterium agent is not used. The decomposing matrix is prepared by adding 1 kilogram of bacterium agent into every 5 square mixture through the specific process and fermentation; the mixture is formed by mixing 40-60% of domestic garbage, 20-40% of sludge and 5-15% of corn straws in percentage by weight; when the matrix is applied in cucumber cultivation, the yield is increased by 11% per mu, the income is increased by 859 yuan, and 200mL of pesticide is saved.
Owner:天津市农业科学院
Biological organic fertilizer for preventing and treating root-knot nematode and preparation method and application of biological organic fertilizer
The invention provides a biological organic fertilizer for preventing and treating root-knot nematode and a preparation method and application of the biological organic fertilizer. The biological organic fertilizer comprises 5-30 parts of a composite bacterial agent and 70-95 parts of a carrier, wherein the composite bacterial agent contains bacillus subtilis, paecilomyces lilacinus, pochonia chlamydospora and streptomycete, and the carrier is made from chicken manure, mushroom residues, and rape seed cake through microorganism compost fermentation. The composite bacterial agent has the effectof preventing and treating root-knot nematode in soil. The carrier is abundant in organic components and inorganic components, which can improve soil, increase the nutritive components in soil, accelerate the effective colonization of the composite bacterial agent in soil, and improve the capability of controlling treating root-knot nematode in soil of the composite bacterial agent. The providedbiological organic fertilizer for preventing and treating root-knot nematode is powder, can be used as a base fertilizer, is harmless to human and animals, is good in preventing and treating effect, and can obviously improve the quality and yield of crops.
Owner:HUNAN TAIGU BIOTECH
Glutamine transaminase zymogen gene for streptomyces hygroscopicus and expression thereof
The invention relates to a transglutaminase zymogen gene of Streptomyces hygroscopicus and the expression thereof, pertaining to molecular biology field. The invention provides the gene sequence of transglutaminase zymogen of Streptomyces hygroscopicus CCTCC M203062 (China patent: 03152956.9, 2003), and further provides a vector system and a host system that has soluble express of the zymogen of the gene code, a part of soluble expressed zymogen can be obtained through the expression system, and the zymogen can be treated by trypsin to prepare activated transglutaminase.
Owner:JIANGNAN UNIV
Strain for producing chitinase and application of strain
ActiveCN109337843APromote growthEasy to trainBacteriaMicroorganism based processesMetapenaeusMicrobiology
The invention relates to a strain for producing chitinase and application of the strain. The strain is classified and named as (Streptomyces diastaticus) CS1801 with a preservation number of CCTCC NO:M2018263. The Streptomyces diastaticus CS1801 comes from naturally-fermented metapenaeus affinis paste. By fermenting the metapenaeus affinis, the enzyme activity of the chitinase is as high as 57.3U / L and the content of chitooligosaccharides is 0.58mol / L. The invention provides a novel method for producing the chitooligosaccharides and has a good application prospect.
Owner:CHANGSHU INSTITUTE OF TECHNOLOGY
Low-temperature resisting method for festuca arundinacea by using complex microbial inoculants
InactiveCN103931664AIncrease chlorophyll contentImprove cold resistancePlant growth regulatorsBiocideBiotechnologyTrichoderma reesei
The invention discloses a low-temperature resisting method for festuca arundinacea by using air-dried sludge inoculants, which is implemented by comprising components including pseudomonas stutzeri, trichoderma reesei and yellow streptomyces 100-times diluent or pseudomonas stutzeri, trichoderma reesei and yellow streptomyces 200-times diluent. Development results of the invention show that the application of the complex microbial inoculants of pseudomonas stutzeri, trichoderma reesei and streptomyces griseoflavus can significantly improve the height, the aboveground biomass and the chlorophyll content of festuca arundinacea plants, and improve the cold resistance thereof. The regreening time of festuca arundinacea to which the complex microbial inoculants are applied is about five days earlier than that in a blank, and the comprehensive index of lawn appearance of regreened festuca arundinacea is also significantly higher than that in the blank, which fully shows the application potential of the complex microbial inoculants in practice.
Owner:TIANJIN NORMAL UNIVERSITY
Composite biological seed coating agent used for controlling vegetable soil-borne diseases and preparation and application methods thereof
The invention discloses a composite biological seed coating agent used for controlling vegetable soil-borne diseases and preparation and application methods thereof; the composite biological seed coating agent comprises bacillus subtilis and streptomyces rochei, also contains water as a carrier medium, sodium carboxymethyl cellulose as an adhesive, sodium lignin sulfonate as a dispersing agent, chitosan and polyvinyl alcohol as a film forming agent, sodium benzoate as a preservative and rhodamine as a colorant; the preparation method is as follows: (1) respectively preparing bacillus subtilis and streptomyces rochei fermentation liquids; (2) stirring and mixing the bacillus subtilis and streptomyces rochei fermentation liquids in a ratio of 2:1; (3) proportion ally adding the sodium carboxymethyl cellulose, the sodium lignin sulfonate, the chitosan, the polyvinyl alcohol, the sodium benzoate and the rhodamine, and finally complementing the margin with the water.; and the application method is as follows: coating, root dipping and root irrigation. The composite biological seed coating agent is obvious in disease resistance effect and long in effective period, has the effects of saving seeds, strengthening seedlings and the like, and is free of environmental pollution.
Owner:JIANGSU LIXIAHE REGION AGRI RES INST
Pig-raising fermentation bacterial agent for biological and environment-friendly fermentation bed and preparation method thereof
The invention discloses a pig-raising fermentation bacterial agent for a biological and environment-friendly fermentation bed and a preparation method thereof. The preparation method comprises the following steps of: firstly selecting seven strains of bacteria, namely Morchella sp. CGMCC (China General Microbiological Culture Collection Center) NO.5.496, Bacillus stearothermophilus CGMCC NO.1.1923, Bacillus stearothermophilus CGMCC NO.1.1866, Bifidobacterium thermoacidophilum CGMCC NO.1.2282, Bacillus subtilis subsp. subtilis CGMCC NO.1.108, Streptomyces anandii CGMCC NO.4.1314, Saccharomycescerevisiae CGMCC NO.2.2081 as production and fermentation parent agents, wherein the viable count of each production and fermentation parent agent is not less than 108 / ml; and then performing freeze-drying on the seven strains of the production and fermentation bacterial agents, fully and uniformly mixing according to the weight of each powder freeze-dried strain, and then performing vacuum packaging to obtain a complex bacterial agent. By using the fermentation bed manufactured by the fermentation bacterial agent, the odor of a pigpen can be obviously reduced, the breeding opportunities of flies and mosquitoes can be reduced, the immunity of pigs can be improved, the healthy growth of the pigs can be protected, and the fermentation bed simultaneously has the characteristics of simplicityin practical operation and simplicity in manufacturing of the fermentation bed.
Owner:WEIWU JINLVYUAN AGRI TECH DEV
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