Domestic garbage and sludge decomposing bacterium agent and application thereof and prepared decomposing matrix

A technology for domestic waste and decomposing inoculants, applied in the application, fungicide, biocide and other directions, can solve the problems of low combustion value of domestic waste, human health threats, human and environmental threats, etc., to improve crop quality, increase Nutrient conversion rate, the effect of reducing the use of chemical fertilizers

Active Publication Date: 2014-08-06
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, the rapid development of Tianjin's economy has brought about a large amount of domestic waste. According to statistics, the total amount of waste in Tianjin has exceeded 2 million tons per year. For this reason, Tianjin has increased the utilization efficiency of waste. Landfilling has gradually developed into a combination of landfill and incineration for power generation, gradually reducing the amount of landfill. Although some waste resources have been recycled after incineration, the fuel value of domestic waste is not high, the water content is large, and the energy conversion rate Low
In addition, after the domestic waste is burned, a large amount of toxic and harmful gas-dioxin will be produced, which poses a serious threat to human health.
In addition, with the acceleration of Tianjin’s rural ecological civilization construction process, a large number of sewage treatment plants have been established in rural areas, correspondingly producing a large amount of sludge. If not properly treated, secondary pollution is likely to occur, causing harm to people and the environment. pose a huge threat

Method used

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  • Domestic garbage and sludge decomposing bacterium agent and application thereof and prepared decomposing matrix
  • Domestic garbage and sludge decomposing bacterium agent and application thereof and prepared decomposing matrix
  • Domestic garbage and sludge decomposing bacterium agent and application thereof and prepared decomposing matrix

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of media and strains

[0040] 1. Preparation of culture medium

[0041] 1. Potato and dextrose agar medium (PDA)

[0042]Take 200 grams of peeled potatoes, cut them into small pieces, add 1000ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000ml, add 20 grams of glucose, 15 grams of agar, dissolve and subpackage, sterilize for 30 minutes in 15 pounds , the pH is natural, and no agar is added when preparing the liquid medium.

[0043] 2. Nutrient gravy agar

[0044] It consists of 5g of peptone, 5g of sodium chloride, 3g of beef extract and 1000ml of distilled water. pH7.0 (add 15-20g of agar to the solid medium).

[0045] 3. Gao's Synthetic Medium No. 1

[0046] KNO 3 1g, K 2 HPO 4 0.5g, NaCl 0.5g, FeSO 4 7H 2 O 0.01g, MgSO 4 7H 2 O 0.5g soluble starch 20g, pH7.2~7.4, distilled water 1000ml.

[0047] 4. Potassium bacteria medium

[0048] Yeast Extract 0.4g, Mannitol 10.0g, K 2 HPO 4 0.5g, MgSO 4...

Embodiment 2

[0066] Composition of compound bacterial agent

[0067] A rural domestic garbage and sludge decomposing bacteria agent is prepared as follows (by weight):

[0068] Table 1 The composition of rural domestic waste and sludge decomposing bacteria agent

[0069] name Bacillus subtilis Streptomyces flavinus Long handle Trichoderma Aspergillus niger Bacillus peptiformis （1） 0.5 2.0 1.0 1.5 2.0 （2） 1.0 1.5 2.0 1.0 1.5 （3） 1.5 1.0 1.5 0.5 1.0 （4） 2.0 0.5 0.5 2.0 0.5

[0070] The number of effective viable bacteria is 5×10 8 piece / g;

Embodiment 3

[0072] Mutual antagonism experiment between strains

[0073] The activated Bacillus subtilis, Streptomyces flavinus, Trichoderma longifolia, Aspergillus niger and Bacillus peptone-like were subjected to the mutual antagonism experiment between the strains, and they were combined in pairs, divided into 20 combinations in total, and each combination had three repeat.

[0074] The steel ring method was used for the antibacterial test, and the strain was cultured in the corresponding medium for 3-7 days (190r / min, 28°C) to make a fermentation broth. Take 0.1mL of the fermentation broth of one of the bacteria (A) and spread it on a PDA plate, then place two pre-sterilized steel circles on the solid medium of each plate, and then add 0.25mL to each steel circle For the fermentation broth of any bacteria (B) other than bacteria A, each treatment was repeated three times, and the plate only inoculated with bacteria A was used as a control. Incubate at 28°C and observe the antibact...

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Abstract

The invention discloses a rural domestic garbage and sludge decomposing bacterium agent and a using method as well as a decomposing matrix prepared by using the bacterium agent and a specific process through fermentation. The bacterium agent is compounded by 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum, 0.5-2 parts of aspergillus nige and 0.5-2 parts of bacillus mucilaginosus in parts by weight; the effective viable count of the bacterium agent is 5*10<8>/g; the bacterium agent can be used for decomposing garbage and sludge, deodorizing, resisting fusarium wilt of cucumber and increasing the yield when being applied to crops; the fermentation period of the treated garbage and sludge is 20 days when the bacterium agent is inoculated and 30 days when the bacterium agent is not used. The decomposing matrix is prepared by adding 1 kilogram of bacterium agent into every 5 square mixture through the specific process and fermentation; the mixture is formed by mixing 40-60% of domestic garbage, 20-40% of sludge and 5-15% of corn straws in percentage by weight; when the matrix is applied in cucumber cultivation, the yield is increased by 11% per mu, the income is increased by 859 yuan, and 200mL of pesticide is saved.

Description

technical field [0001] The invention belongs to the field of application of agricultural microorganisms in treating rural domestic garbage and sludge, and relates to a decomposing bacteria agent for rural domestic garbage and sludge, a use method thereof and a prepared decomposing substrate. Background technique [0002] In recent years, the rapid development of Tianjin's economy has brought about a large amount of domestic waste. According to statistics, the total amount of waste in Tianjin has exceeded 2 million tons per year. For this reason, Tianjin has increased the utilization efficiency of waste. Landfilling has gradually developed into a combination of landfill and incineration for power generation, gradually reducing the amount of landfill. Although some waste resources have been recycled after incineration, the fuel value of domestic waste is not high, the water content is large, and the energy conversion rate Low. In addition, after domestic waste is burned, a la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F17/00C05F15/00A01P3/00A01P21/00C12R1/125C12R1/465C12R1/885C12R1/685C12R1/07
CPCY02W30/40
Inventor 何宗均田阳梁海恬赵琳娜
Owner 天津市农业科学院
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