31 results about "Trichoderma longibrachiatum" patented technology

The invention relates to a growth-promoting compound microbial agent, which is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and is characterized in that the growth-promoting compound microbial agent is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and the growth-promoting compound microbial agent is prepared from bacillus amyloliquefaciens, bacillus subtilis and Trichoderma longibrachiatum. The compound microbial agent (bacillus subtilis, pseudofungal bacillus and trichoderma longibrachiatum) has excellent performance in the aspects of promoting plant growth and improving the soil environment, and the plant height, the fresh weight and the root length of plants are remarkably increased. The application of the complex microbial inoculant effectively relieves the chive continuous cropping obstacle, improves the chive growth potential and soil fertility, and provides a theoretical basis and technical support for sustainable planting of chives in facility agriculture.

The present application relates to a kind of bacteria inhibiting and growth promoting microorganism and its compound microbial inoculant, preparation method and application.The present application provides a kind of bacillus subtilis (Bacillus subtilis) YCBA0319, preservation number is CGMCC No.33006;A kind of trichoderma longibrachiatum (Trichoderma longibrachiatum) YCFA0522, preservation number is CGMCC No.41655.In the technical solution of the present application, through bacillus subtilis and trichoderma longibrachiatum and the compound microbial inoculant of its composition, through the synergistic effect of two strains, it has the promotion effect to soil health and apple yield, quality etc., compared with control, soil bulk density is reduced, organic matter and available nutrient are improved, and apple single fruit size, yield and quality are all improved.

The invention discloses trichoderma longibrachiatum for degrading petroleum hydrocarbon in a saline-alkali environment, a culture and application of the trichoderma longibrachiatum, and relates to the technical field of microorganisms. The preservation number of the trichoderma longibrachiatum is CGMCC (China General Microbiological Culture Collection Center) No. The trichoderma longibrachiatum provided by the invention has an efficient petroleum degradation characteristic, provides a practical and effective solution for the petroleum pollution problem of the saline-alkali soil, can efficiently degrade petroleum hydrocarbon in the saline-alkali soil, and has a good application prospect.

The present application belongs to the field of biotechnology, and particularly relates to a preparation method of a taiwanofungus castaneus enzyme Trichoderma longibrachiatum and bacillus licheniformis Bacillus licheniformis are mixed to prepare a composite fermentation seed liquid. A culture medium composed of cassava residues and sawdust is used to prepare pretreated taiwanofungus castaneus culture medium. Then, the taiwanofungus castaneus strain is inoculated in the culture medium for fermentation, and a heat stress treatment step is introduced in the fermentation process. The method significantly promotes the synthesis and dissolution of active components of taiwanofungus castaneus triterpenoids and polysaccharides by the synergistic effect of specific strains and the combination of physical stress. The present application solves the technical problem of insufficient dissolution of active substances of taiwanofungus castaneus in the prior art by a unique strain combination and process design, and has high application value and market prospect.

The invention relates to a complex microbial inoculant for mixed compost of sugarcane filter mud and sugarcane leaves and an application method, and belongs to the technical field of microorganisms, the complex microbial inoculant comprises a high-temperature microbial inoculant, and the high-temperature microbial inoculant is prepared from thermophilic carbon monoxide streptomyces G-4-7, brevibacillus brevis G-5-2 and thermophilic chaetomium; the medium-temperature microbial inoculum is prepared from trichoderma longibrachiatum, staphylococcus warneri LH-403 and bacillus thuringiensis LMU-81, and the medium-temperature microbial inoculum is prepared from the trichoderma longibrachiatum and the staphylococcus warneri LH-403; the complex microbial inoculant adopts a two-stage inoculation method. The complex microbial inoculant can effectively enhance degradation of lignocellulose so as to promote compost maturity, the humification degree of compost is further improved through the biological enhancement effect, the nitrogen fixation effect of the compost is facilitated, the germination index of the compost is improved, resource utilization of organic matter rich in lignocellulose can be promoted, and the environment-friendly effect is achieved. And a technical basis is provided for a green treatment mode of agricultural wastes.

The present application relates to the technical field of microbial degradation of rhizoma polygoni multifidi residues, and discloses a composite microbial agent and a preparation method and application thereof.The composite microbial agent contains trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis; wherein the proportion of the spores or spores of the trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis in the composite microbial agent is 1-6:1-6:1-6:1-6:1-6:1-6:1-6.Based on this, the composite microbial agent has good degradation efficiency on rhizoma polygoni multifidi residues, can accelerate the composting process of rhizoma polygoni multifidi residues, shorten the composting period of rhizoma polygoni multifidi residues, and the composting product after composting is stable in quality and is a high-quality organic fertilizer, so that effective utilization of resources is realized.

The invention discloses a method for preparing an organic fertilizer by co-composting double wastes in the wine brewing industry, which comprises the following steps: mixing 50-90 parts by mass of vinasse, 10-30 parts by mass of livestock and poultry manure and 10-30 parts by mass of plant ash to obtain a compost substrate, adding a compound fermentation inoculant accounting for 0.02-1.0% of the total mass of the compost substrate, and uniformly stirring to obtain the organic fertilizer. The compound fermentation inoculant comprises bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, aspergillus niger and trichoderma longibrachiatum, and the viable count in the compound fermentation inoculant is greater than or equal to 1 * 10 < 10 > CFU / g; composting is carried out by adding the composting substrate of the composite fermentation inoculant, first pile turning is started when the average temperature rises to 50 DEG C, pile turning is carried out once every 5-10 days, and yellow serofluid is added to maintain the humidity of a pile body at 55-65%. According to the method disclosed by the invention, through precise adaptation of characteristics of the composite fermentation inoculant and double wastes, synergistic efficient degradation of the vinasse and the yellow serofluid is realized, and the treatment cost is reduced.

The invention discloses a growth-promoting bacterial fertilizer for pandan leaves and application of the growth-promoting bacterial fertilizer, and relates to the technical field of biology. Active components of the growth-promoting bacterial fertilizer for the pandan leaves comprise a microorganism combination, the microbial composition comprises bacillus altitudinis, bacillus megatherium, trichoderma longibrachiatum and aspergillus parasuis. Through the synergistic effect of the four strains, the growth-promoting bacterial fertilizer can effectively improve the soil environment of the saline-alkali soil, reduce the pH value of the soil and improve the enzyme activity of the soil and the nutrient effectiveness of the soil; meanwhile, growth and development of pandan leaves can be remarkably promoted, and plant height, stem diameter, fresh weight of single plant and acre yield are increased; the quality of pandan leaves can be improved, and the content of a characteristic aroma substance 2-acetyl-1-pyrroline is increased. The growth-promoting bacterial fertilizer is simple in preparation process, relatively low in cost, environment-friendly and particularly suitable for popularization and application in planting of pandan leaves in saline-alkali soil, and has important agricultural application value and market prospect.

This invention discloses an organic fertilizer for increasing guava yield in saline-alkali land and its preparation method, relating to the field of biotechnology. The active ingredients of this organic fertilizer include *Bacillus horikoshii* ACCC 01061, *Bacillus safensis* CICC 21743, *Sporidiobolus salmonicolor* CICC 32895, *Trichoderma koningii* CICC 13006, and *Trichoderma longibrachiatum* ACCC 32095. This organic fertilizer can improve the yield per guava tree and the quality of the fruit grown in saline-alkali land, and has significant market application value.

This invention belongs to the field of agricultural microbiology technology and relates to the longibrachiatum strain ZN25, which exhibits broad-spectrum resistance to strawberry diseases, and its applications. The longibrachiatum strain ZN25 is classified as *Trichoderma longibrachiatum* and has the accession number CGMCC No. 42426. This strain, isolated from the strawberry endophytic environment, exhibits broad-spectrum resistance to the pathogens of strawberry anthracnose (*Anthracnose sianna*) and root rot (*Fusarium oxysporum*). It inhibits pathogen growth through multiple mechanisms and simultaneously produces a series of volatile gaseous substances with antibacterial activity, enabling green and efficient control of strawberry anthracnose and root rot.

The present application relates to mould inhibition technology and desulfurization wastewater treatment field, especially to a kind of mould inhibitor and its preparation method and application, including desulfurization wastewater 90-110 parts, chitosan 8-12 parts, sodium lignosulfonate 5-8 parts, quaternary ammonium salt disinfectant 5-10 parts and anionic flocculant 0.001-0.002 parts.Desulfurization wastewater contains higher content of chloride ion, and various heavy metal ions, including but not limited to mercury, copper, iron, zinc and lead, so high cost is needed to treat desulfurization wastewater every year, the present application uses desulfurization wastewater as the main component of mould inhibitor, and is supplemented with chitosan, sodium lignosulfonate, quaternary ammonium salt disinfectant and anionic flocculant, the prepared mould inhibitor not only has long-term efficient removal and inhibition effect of mould, but also reduces the treatment cost of desulfurization wastewater and the production cost of mould inhibitor.The mould inhibitor of the present application has long-term efficient removal and inhibition effect on common moulds such as aspergillus niger, trichoderma longibrachiatum and paecilomyces variotii.

The invention relates to a Trichoderma longibrachiatum strain TV26, a fungicide containing the Trichoderma longibrachiatum strain TV26 and application of the The trichoderma longibrachiatum strain TV26 has an efficient and lasting antagonistic effect on cucurbitaceae, and can play a key role in biological prevention and control of cucumber spot disease. Furthermore, by researching endogenous metabolites of the strain, it is found that the volatile metabolite pinobiose of the strain serves as a synergist, the colonization ability of the strain on cucumber leaves is remarkably improved, and therefore the strain can be used for developing a more efficient compound microbial agent. The bacterial strain and the compound bacterial agent have the characteristics of high efficiency, durability, safety and environmental protection when being used for antagonizing cucurbitaceae and preventing and treating cucumber spot disease caused by cucurbitaceae, so that the bacterial strain and the compound bacterial agent have relatively high industrial value and application prospect.

The invention discloses a crop straw rapid decomposition bacterial agent for sandy soil and a fermentation process thereof, and particularly relates to the field of agricultural microorganisms, and the bacterial agent is prepared by compounding trichoderma viride, bacillus pumilus, trichoderma longibrachiatum and bacillus subtilis according to the volume ratio of 1: 1: 2: 2. According to the preparation process, a solid culture medium taking straw as a main material is adopted, and mixed solid fermentation is performed through layered inoculation, staged variable temperature control, humidity regulation and intermittent ventilation, so that the final product has high viable count and strong enzyme activity, and can keep high activity in a severe environment of sandy soil; the straw decomposition process is quickly started and completed; the microbial inoculum can efficiently and rapidly decompose crop straws in sandy soil, significantly improve humification efficiency and soil fertility, effectively improve soil organic matter and nutrient content, improve soil structure and promote crop yield increase, and has important application value and market prospect.

The present application relates to the technical field of sugarcane smut prevention and treatment, and particularly relates to a microbial fertilizer, a preparation method thereof and application thereof in prevention and treatment of smut. The microbial fertilizer comprises, by weight, 40-60 parts of cow dung, 2-4 parts of corn straw biochar, 4-6 parts of mulberry branch and 8-14 parts of mixed bacterial agent. The mixed bacterial agent is composed of Bacillus subtilis X1, Bacillus altitudinis X2, Bacillus cereus X3, Trichoderma harzianum Z1 and Trichoderma longibrachiatum Z2. The microbial fertilizer has a positive promoting effect on sugarcane and can significantly stimulate the self-defense response of sugarcane.

The invention belongs to the technical field of microbial agents, and particularly relates to trichoderma longibrachiatum MR-Z1 and application thereof. The trichoderma longibrachiatum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 24, 2025, and the preservation number of the trichoderma longibrachiatum is CGMCC No. 41925. The Trichoderma longibrachiatum MR-Z1 strain provided by the invention has an obvious promotion effect on soybean growth and nodulation condition improvement under a pot experiment condition, so that soybean root development, nodulation nitrogen fixation and stress resistance are regulated and controlled in multiple dimensions.

The invention belongs to the technical field of fermentation, and particularly relates to a method for inducing generation of water-soluble pachymaran. According to the method, Wolfiporia cocos and Trichoderma longibrachiatum are subjected to staged co-culture, and low-temperature stress at the temperature of 15 DEG C is applied to the key stage of co-culture. The specific strain combination and low-temperature induction have a synergistic effect, so that a water-soluble polysaccharide synthesis and secretion pathway of the poria cocos bacteria is remarkably activated, and the technical bottlenecks of low water-soluble polysaccharide proportion and low yield in the existing fermentation process are solved. The yield of the prepared water-soluble pachymaran can reach 2.35 g / L, the immunoregulatory activity of the water-soluble pachymaran is excellent, macrophages can be effectively promoted to secrete key cell factors such as IL-6 and IL-1beta, and a new technical scheme is provided for developing efficient and high-value pachymaran products.

The present application relates to the field of microorganism and biomass processing, and specifically discloses a solid-state fermentation of straw of Trichoderma longibrachiatum and a fermentation process thereof. The present application uses normal temperature and pressure plasma mutagenesis technology to improve the Trichoderma longibrachiatum, and obtains a high-efficiency lignocellulose-degrading mutant strain through a hierarchical screening system. Compared with the original strain, the mutant strain shows a significant improvement in lignocellulose-degrading enzyme activity and protein accumulation. Experiments show that the fermentation process can make the true protein content of corn straw reach 17.57%, and the degradation rates of cellulose, hemicellulose and lignin reach 24.89%, 23.54% and 22.28% respectively. The present application realizes the efficient bioconversion of corn straw, and provides a solution for the resource utilization of agricultural waste.

This invention discloses the application of Trichoderma composting agents in compost preparation and a method for compost preparation, belonging to the field of biocomposting technology. It provides the application of Trichoderma composting agents in compost preparation, wherein the Trichoderma composting agents include *Trichoderma longibrachiatum* T6, *Trichoderma viride* P6, and *Trichoderma longibrachiatum* Lu3. *Trichoderma longibrachiatum* T6 is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC NO. 13183 and deposit date November 14, 2016. This invention utilizes Trichoderma composting agents and an aerobic composting method to greatly increase the activity of *Trichoderma* fungi in the compost. Regular aeration and constant temperature composting significantly accelerate the composting speed of straw and cow manure.

The invention belongs to the technical field of fermentation engineering, and relates to a method for cultivating bacteria in an airing room and application thereof. A method for cultivating bacteria in an airing hall comprises the following steps that S1, high-temperature Daqu waste grass and high-temperature large-area powder are matched according to the mass ratio of 5: 1 and laid on the ground of the airing hall, a waste grass ground layer is formed, and the laying thickness of the waste grass ground layer is 5-8 cm; s2, the surface of the waste grass ground layer is covered with a heat preservation layer, and the temperature of the waste grass ground layer is kept at 40-45 DEG C for no less than 7 days; s3, removing the thermal insulation layer, and laying the fermented grains with the temperature lower than 30 DEG C on the waste grass ground layer to form a microbial colonization layer with the thickness of 15-20 cm; and S4, the waste grass ground layer is removed, an inorganic salt solution with the mass fraction of 0.1-0.3% is sprayed and supplemented to the ground of the airing hall, and liquid supplementing is continuously conducted for 14-21 days. According to the method, the activity of ground microorganisms is rapidly activated by utilizing residual nutrients of waste grass and inherent flora, free flora in the environment is adsorbed, proliferation is promoted, straw resource utilization is achieved, and the cost of a bacterium culture cycle is reduced.

The invention discloses a Wolfiporia cocos Wc2408, the preservation number of the Wolfiporia cocos Wc2408 in the China Center for Type Culture Collection is CCTCC NO: M 2026123, and in a plate confrontation experiment, the strain can still keep normal growth and cover a mould colony in the presence of soil competitive fungi represented by trichoderma longibrachiatum; the strain is applied to poria cocos cultivation, compared with a commercial strain, the strain has the capacity of resisting infection of infectious microbes such as trichoderma longibrachiatum and the like, the strain has the high poria cocos fruiting rate and the single plant yield, and the strain with the high space competition capacity, the stress resistance and the high poria cocos yield is provided for poria cocos production. And the method is suitable for large-scale production and market promotion.

The utility model relates to the technical field of crop disease prevention, in particular to a device for preventing and treating crop diseases by utilizing trichoderma longibrachiatum. The device comprises a storage tank, a water pump part is fixedly installed in the center of the top side of the storage tank and communicates with the interior of the storage tank, and an adjusting assembly is arranged in the center of the top side of the water pump part and comprises a connecting pipe. Through the arranged adjusting assembly, the twisting strip is used for driving the external thread shell to rotate on the outer side of the connecting pipe, the internal thread ring is driven to vertically move on the outer side of the external thread shell through rotation of the external thread shell, and meanwhile the multiple L-shaped fixing strips are driven to vertically move on the outer side of the internal thread ring; and when the L-shaped fixing strip vertically moves, through cooperation of the fixing strip and the sliding ring, the spraying heads rotate between the two supporting strips, so that the effect of adjusting the angles of the multiple spraying heads can be achieved, and the situation that spraying is not uniform enough due to the fact that the angles are too large or too small is avoided.

The invention discloses staphylococcus warneri as well as a complex microbial inoculant, a preparation method and application thereof, and belongs to the technical field of microorganisms, the staphylococcus warneri has the strain name of LH-403 and the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36214, and the complex microbial inoculant is prepared from the staphylococcus warneri, Trichoderma longibrachiatum and Bacillus thuringiensis LMU-81 together. According to the invention, the three strains are compounded to generate a synergistic effect, so that the lignocellulase can be massively produced at normal temperature, and the method is suitable for degradation of bagasse lignocellulose.

The invention provides a seed coating agent as well as a preparation method and application thereof, and belongs to the technical field of crop growth promotion. The seed coating agent comprises a microbial agent and a film-forming agent; the microbial agent comprises any two or three of bacillus velezensis, bacillus subtilis and trichoderma longibrachiatum. The seed coating agent is obtained by combining the microbial agent provided by the invention with a film-forming agent. The seed coating agent is stable in physicochemical property, the pH value is 7-8, the coating uniformity is larger than or equal to 90%, the coating falling rate is smaller than or equal to 8%, and the seed coating agent has high-temperature and low-temperature stability. After seeds are coated with the seed coating agent, the germination rate of the seeds can be remarkably increased, and the germination period of the seeds can be shortened; the growth of the seedlings is obviously promoted, the overground part length and the underground part length of the seedlings are increased, the dry weight and the fresh weight of the seedlings are increased, and the root-shoot ratio of the seedlings is increased. In conclusion, the seed coating agent provided by the invention has high germination rate, stability and growth promoting ability, and a theoretical basis is provided for development and application of the microbial seed coating agent.

The invention belongs to the technical field of biology, and particularly relates to a preparation method of antrodia camphorata ferment. According to the method, Trichoderma longibrachiatum and Bacillus licheniformis are mixed to prepare a composite fermentation seed solution, the composite fermentation seed solution is used for carrying out composite fermentation on a culture medium composed of cassava residues and wood chips, and the pretreated antrodia camphorata culture medium is prepared. Then, inoculating an antrodia camphorata strain into the culture medium for fermentation, and introducing a heat stress treatment step in the fermentation process; according to the method, synthesis and dissolution of triterpenoids and polysaccharide active ingredients of antrodia camphorata are remarkably promoted through combination of the synergistic effect of specific strains and physical stress, the technical problem that in the prior art, active substances of antrodia camphorata are not sufficiently dissolved out is solved through unique strain combination and process design, and the method has extremely high application value and market prospects.

The invention discloses a tea tree culture medium and application thereof, and relates to the technical field of biology. The tea tree culture medium comprises a basic culture medium and a microorganism combination, the microbial composition comprises bacillus niger and trichoderma longibrachiatum. The culture medium with air permeability, water-retaining property and fertility is constructed through scientific proportioning, and meanwhile, microorganisms are uniformly colonized in the medium by adding a microorganism combination, so that growth and diffusion of root cancer pathogenic bacteria can be continuously inhibited. Experimental data show that the survival rate of the tea trees cultivated by adopting the tea tree cultivation medium is effectively improved, the root activity is remarkably enhanced, and the dual purposes of disease prevention and control and high quality and high yield of the tea trees are achieved. According to the technical scheme, microbial ecological regulation and control and substrate physical and chemical improvement are combined, and a green and sustainable solution is provided for tea tree planting.

The invention belongs to the field of agricultural planting, and particularly relates to a cultivation method for improving the yield and quality of feeding oats in saline-alkali soil. The invention discloses a cultivation method for improving the yield and quality of feeding oats in saline-alkali soil, which comprises the following steps: in the growth period of oats, drip irrigation is combined with fertilization to improve the yield and quality of oats, the drip irrigation is ground drip irrigation, the interval of drip irrigation belts is 55 cm, the interval of water droppers is 30 cm, irrigation is carried out for five times, and 60 mm of water is irrigated for each time. In the step of fertilization, a biological bacterial fertilizer is applied as a base fertilizer, a water-soluble fertilizer is applied in combination, the water-soluble fertilizer is applied for four times, and the biological bacterial fertilizer is a biological bacterial fertilizer containing plant lactobacillus CICC 10345 and trichoderma longibrachiatum CGMCC 3.6599. Field test results show that the method for improving the yield and quality of the oats in the saline-alkali soil can improve the yield of the oats in the saline-alkali soil and improve the content of crude protein and crude fat in the oats. The invention provides a new thought for healthy development of the saline-alkali soil oat industry.

The present application relates to a strain of Trichoderma longibrachiatum TV26, a microbial agent comprising the same and an application thereof. The strain of Trichoderma longibrachiatum TV26 has a high and persistent antagonistic effect on Pseudomonas lachrymans, and can play a key role in the biological control of cucumber spot disease. Further, through the study of its endogenous metabolites, it is found that the volatile metabolite of turanose as a synergist can significantly improve the colonization ability of the strain on cucumber leaves, so as to be used for developing a more efficient compound microbial agent. Whether the strain of the present application or the compound microbial agent thereof has the characteristics of high efficiency, persistence, safety and environmental protection when used for antagonizing Pseudomonas lachrymans and preventing cucumber spot disease caused by Pseudomonas lachrymans, and therefore has high industrial value and application prospect.

The invention belongs to the technical field of microorganisms, and relates to salt-tolerant trichoderma longibrachiatum and application thereof. The preservation number of the trichoderma longibrachiatum CH-Z1 is CGMCC No. 41752, and the trichoderma longibrachiatum CH-Z1 can improve the salt stress resistance of plants. The trichoderma longibrachiatum CH-Z1 obtained through screening can remarkably inhibit peanut root rot bacteria, peanut black rot bacteria, peanut sclerotium rolfsii and peanut fruit rot bacteria and promote growth of peanut seedlings, is a biocontrol strain with broad-spectrum antibacterial ability and growth promoting effect, and has the advantages of being environmentally friendly, high in specificity, environmentally friendly and the like; and the strain can improve the salt stress resistance of peanut plants and improve the salt-alkali environment, and has important significance for guaranteeing the yield and quality safety of peanuts.

The present invention belongs to the field of microorganisms, and specifically relates to a strain of Trichoderma longibrachiatum CM5J-9 that is highly effective in antagonizing strawberry diseases, and its screening method and application. The Trichoderma longibrachiatum CM5J-9 is deposited with the CGMCC NO.41989 on May 30, 2025, at the General Microbiology Center of the China Culture Collection Administration, and is classified as: Trichoderma longibrachiatum Trichoderma longibrachiatum . The long-branch Trichoderma CM5J-9 screened by the present invention has a significant antagonistic effect on the main pathogens in the soil of continuous strawberry planting, and has a significant preventive and control effect on strawberry-related diseases caused by them. The long-branch Trichoderma CM5J-9 provided by the present invention is isolated from the soil of continuous strawberry planting, has good environmental adaptability and disease prevention effect, and has a large spore production amount, and has great application potential.

This invention discloses a bamboo vinegar powder extract and its identification method and application. The bamboo vinegar powder extract contains at least one of pyrogallol, 4-methylcatechol, p-hydroxyphenylethanol, p-methylbenzoic acid, dihydrocaffeic acid, and 1,4-dibenzene. Among them, 4-methylcatechol and p-methylbenzoic acid have broad-spectrum antifungal effects, exhibiting very good antifungal activity against Aspergillus terreus CICC40377, Trichoderma longibrachiatum CICC40380, and Paecilomyces variotii CICC40716.