22 results about "Trichoderma longibrachiatum" patented technology

The present application relates to a kind of bacteria inhibiting and growth promoting microorganism and its compound microbial inoculant, preparation method and application.The present application provides a kind of bacillus subtilis (Bacillus subtilis) YCBA0319, preservation number is CGMCC No.33006;A kind of trichoderma longibrachiatum (Trichoderma longibrachiatum) YCFA0522, preservation number is CGMCC No.41655.In the technical solution of the present application, through bacillus subtilis and trichoderma longibrachiatum and the compound microbial inoculant of its composition, through the synergistic effect of two strains, it has the promotion effect to soil health and apple yield, quality etc., compared with control, soil bulk density is reduced, organic matter and available nutrient are improved, and apple single fruit size, yield and quality are all improved.

The invention discloses trichoderma longibrachiatum for degrading petroleum hydrocarbon in a saline-alkali environment, a culture and application of the trichoderma longibrachiatum, and relates to the technical field of microorganisms. The preservation number of the trichoderma longibrachiatum is CGMCC (China General Microbiological Culture Collection Center) No. The trichoderma longibrachiatum provided by the invention has an efficient petroleum degradation characteristic, provides a practical and effective solution for the petroleum pollution problem of the saline-alkali soil, can efficiently degrade petroleum hydrocarbon in the saline-alkali soil, and has a good application prospect.

The present application belongs to the field of biotechnology, and particularly relates to a preparation method of a taiwanofungus castaneus enzyme Trichoderma longibrachiatum and bacillus licheniformis Bacillus licheniformis are mixed to prepare a composite fermentation seed liquid. A culture medium composed of cassava residues and sawdust is used to prepare pretreated taiwanofungus castaneus culture medium. Then, the taiwanofungus castaneus strain is inoculated in the culture medium for fermentation, and a heat stress treatment step is introduced in the fermentation process. The method significantly promotes the synthesis and dissolution of active components of taiwanofungus castaneus triterpenoids and polysaccharides by the synergistic effect of specific strains and the combination of physical stress. The present application solves the technical problem of insufficient dissolution of active substances of taiwanofungus castaneus in the prior art by a unique strain combination and process design, and has high application value and market prospect.

The invention relates to a complex microbial inoculant for mixed compost of sugarcane filter mud and sugarcane leaves and an application method, and belongs to the technical field of microorganisms, the complex microbial inoculant comprises a high-temperature microbial inoculant, and the high-temperature microbial inoculant is prepared from thermophilic carbon monoxide streptomyces G-4-7, brevibacillus brevis G-5-2 and thermophilic chaetomium; the medium-temperature microbial inoculum is prepared from trichoderma longibrachiatum, staphylococcus warneri LH-403 and bacillus thuringiensis LMU-81, and the medium-temperature microbial inoculum is prepared from the trichoderma longibrachiatum and the staphylococcus warneri LH-403; the complex microbial inoculant adopts a two-stage inoculation method. The complex microbial inoculant can effectively enhance degradation of lignocellulose so as to promote compost maturity, the humification degree of compost is further improved through the biological enhancement effect, the nitrogen fixation effect of the compost is facilitated, the germination index of the compost is improved, resource utilization of organic matter rich in lignocellulose can be promoted, and the environment-friendly effect is achieved. And a technical basis is provided for a green treatment mode of agricultural wastes.

The present application relates to the technical field of microbial degradation of rhizoma polygoni multifidi residues, and discloses a composite microbial agent and a preparation method and application thereof.The composite microbial agent contains trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis; wherein the proportion of the spores or spores of the trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis in the composite microbial agent is 1-6:1-6:1-6:1-6:1-6:1-6:1-6.Based on this, the composite microbial agent has good degradation efficiency on rhizoma polygoni multifidi residues, can accelerate the composting process of rhizoma polygoni multifidi residues, shorten the composting period of rhizoma polygoni multifidi residues, and the composting product after composting is stable in quality and is a high-quality organic fertilizer, so that effective utilization of resources is realized.

The invention discloses a growth-promoting bacterial fertilizer for pandan leaves and application of the growth-promoting bacterial fertilizer, and relates to the technical field of biology. Active components of the growth-promoting bacterial fertilizer for the pandan leaves comprise a microorganism combination, the microbial composition comprises bacillus altitudinis, bacillus megatherium, trichoderma longibrachiatum and aspergillus parasuis. Through the synergistic effect of the four strains, the growth-promoting bacterial fertilizer can effectively improve the soil environment of the saline-alkali soil, reduce the pH value of the soil and improve the enzyme activity of the soil and the nutrient effectiveness of the soil; meanwhile, growth and development of pandan leaves can be remarkably promoted, and plant height, stem diameter, fresh weight of single plant and acre yield are increased; the quality of pandan leaves can be improved, and the content of a characteristic aroma substance 2-acetyl-1-pyrroline is increased. The growth-promoting bacterial fertilizer is simple in preparation process, relatively low in cost, environment-friendly and particularly suitable for popularization and application in planting of pandan leaves in saline-alkali soil, and has important agricultural application value and market prospect.

This invention discloses an organic fertilizer for increasing guava yield in saline-alkali land and its preparation method, relating to the field of biotechnology. The active ingredients of this organic fertilizer include *Bacillus horikoshii* ACCC 01061, *Bacillus safensis* CICC 21743, *Sporidiobolus salmonicolor* CICC 32895, *Trichoderma koningii* CICC 13006, and *Trichoderma longibrachiatum* ACCC 32095. This organic fertilizer can improve the yield per guava tree and the quality of the fruit grown in saline-alkali land, and has significant market application value.

This invention belongs to the field of agricultural microbiology technology and relates to the longibrachiatum strain ZN25, which exhibits broad-spectrum resistance to strawberry diseases, and its applications. The longibrachiatum strain ZN25 is classified as *Trichoderma longibrachiatum* and has the accession number CGMCC No. 42426. This strain, isolated from the strawberry endophytic environment, exhibits broad-spectrum resistance to the pathogens of strawberry anthracnose (*Anthracnose sianna*) and root rot (*Fusarium oxysporum*). It inhibits pathogen growth through multiple mechanisms and simultaneously produces a series of volatile gaseous substances with antibacterial activity, enabling green and efficient control of strawberry anthracnose and root rot.

The present application relates to mould inhibition technology and desulfurization wastewater treatment field, especially to a kind of mould inhibitor and its preparation method and application, including desulfurization wastewater 90-110 parts, chitosan 8-12 parts, sodium lignosulfonate 5-8 parts, quaternary ammonium salt disinfectant 5-10 parts and anionic flocculant 0.001-0.002 parts.Desulfurization wastewater contains higher content of chloride ion, and various heavy metal ions, including but not limited to mercury, copper, iron, zinc and lead, so high cost is needed to treat desulfurization wastewater every year, the present application uses desulfurization wastewater as the main component of mould inhibitor, and is supplemented with chitosan, sodium lignosulfonate, quaternary ammonium salt disinfectant and anionic flocculant, the prepared mould inhibitor not only has long-term efficient removal and inhibition effect of mould, but also reduces the treatment cost of desulfurization wastewater and the production cost of mould inhibitor.The mould inhibitor of the present application has long-term efficient removal and inhibition effect on common moulds such as aspergillus niger, trichoderma longibrachiatum and paecilomyces variotii.

The invention relates to a Trichoderma longibrachiatum strain TV26, a fungicide containing the Trichoderma longibrachiatum strain TV26 and application of the The trichoderma longibrachiatum strain TV26 has an efficient and lasting antagonistic effect on cucurbitaceae, and can play a key role in biological prevention and control of cucumber spot disease. Furthermore, by researching endogenous metabolites of the strain, it is found that the volatile metabolite pinobiose of the strain serves as a synergist, the colonization ability of the strain on cucumber leaves is remarkably improved, and therefore the strain can be used for developing a more efficient compound microbial agent. The bacterial strain and the compound bacterial agent have the characteristics of high efficiency, durability, safety and environmental protection when being used for antagonizing cucurbitaceae and preventing and treating cucumber spot disease caused by cucurbitaceae, so that the bacterial strain and the compound bacterial agent have relatively high industrial value and application prospect.

The present application relates to the technical field of sugarcane smut prevention and treatment, and particularly relates to a microbial fertilizer, a preparation method thereof and application thereof in prevention and treatment of smut. The microbial fertilizer comprises, by weight, 40-60 parts of cow dung, 2-4 parts of corn straw biochar, 4-6 parts of mulberry branch and 8-14 parts of mixed bacterial agent. The mixed bacterial agent is composed of Bacillus subtilis X1, Bacillus altitudinis X2, Bacillus cereus X3, Trichoderma harzianum Z1 and Trichoderma longibrachiatum Z2. The microbial fertilizer has a positive promoting effect on sugarcane and can significantly stimulate the self-defense response of sugarcane.

The present application relates to the field of microorganism and biomass processing, and specifically discloses a solid-state fermentation of straw of Trichoderma longibrachiatum and a fermentation process thereof. The present application uses normal temperature and pressure plasma mutagenesis technology to improve the Trichoderma longibrachiatum, and obtains a high-efficiency lignocellulose-degrading mutant strain through a hierarchical screening system. Compared with the original strain, the mutant strain shows a significant improvement in lignocellulose-degrading enzyme activity and protein accumulation. Experiments show that the fermentation process can make the true protein content of corn straw reach 17.57%, and the degradation rates of cellulose, hemicellulose and lignin reach 24.89%, 23.54% and 22.28% respectively. The present application realizes the efficient bioconversion of corn straw, and provides a solution for the resource utilization of agricultural waste.

The invention discloses a Wolfiporia cocos Wc2408, the preservation number of the Wolfiporia cocos Wc2408 in the China Center for Type Culture Collection is CCTCC NO: M 2026123, and in a plate confrontation experiment, the strain can still keep normal growth and cover a mould colony in the presence of soil competitive fungi represented by trichoderma longibrachiatum; the strain is applied to poria cocos cultivation, compared with a commercial strain, the strain has the capacity of resisting infection of infectious microbes such as trichoderma longibrachiatum and the like, the strain has the high poria cocos fruiting rate and the single plant yield, and the strain with the high space competition capacity, the stress resistance and the high poria cocos yield is provided for poria cocos production. And the method is suitable for large-scale production and market promotion.

The invention discloses staphylococcus warneri as well as a complex microbial inoculant, a preparation method and application thereof, and belongs to the technical field of microorganisms, the staphylococcus warneri has the strain name of LH-403 and the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36214, and the complex microbial inoculant is prepared from the staphylococcus warneri, Trichoderma longibrachiatum and Bacillus thuringiensis LMU-81 together. According to the invention, the three strains are compounded to generate a synergistic effect, so that the lignocellulase can be massively produced at normal temperature, and the method is suitable for degradation of bagasse lignocellulose.

The invention provides a seed coating agent as well as a preparation method and application thereof, and belongs to the technical field of crop growth promotion. The seed coating agent comprises a microbial agent and a film-forming agent; the microbial agent comprises any two or three of bacillus velezensis, bacillus subtilis and trichoderma longibrachiatum. The seed coating agent is obtained by combining the microbial agent provided by the invention with a film-forming agent. The seed coating agent is stable in physicochemical property, the pH value is 7-8, the coating uniformity is larger than or equal to 90%, the coating falling rate is smaller than or equal to 8%, and the seed coating agent has high-temperature and low-temperature stability. After seeds are coated with the seed coating agent, the germination rate of the seeds can be remarkably increased, and the germination period of the seeds can be shortened; the growth of the seedlings is obviously promoted, the overground part length and the underground part length of the seedlings are increased, the dry weight and the fresh weight of the seedlings are increased, and the root-shoot ratio of the seedlings is increased. In conclusion, the seed coating agent provided by the invention has high germination rate, stability and growth promoting ability, and a theoretical basis is provided for development and application of the microbial seed coating agent.

The invention belongs to the technical field of biology, and particularly relates to a preparation method of antrodia camphorata ferment. According to the method, Trichoderma longibrachiatum and Bacillus licheniformis are mixed to prepare a composite fermentation seed solution, the composite fermentation seed solution is used for carrying out composite fermentation on a culture medium composed of cassava residues and wood chips, and the pretreated antrodia camphorata culture medium is prepared. Then, inoculating an antrodia camphorata strain into the culture medium for fermentation, and introducing a heat stress treatment step in the fermentation process; according to the method, synthesis and dissolution of triterpenoids and polysaccharide active ingredients of antrodia camphorata are remarkably promoted through combination of the synergistic effect of specific strains and physical stress, the technical problem that in the prior art, active substances of antrodia camphorata are not sufficiently dissolved out is solved through unique strain combination and process design, and the method has extremely high application value and market prospects.

The invention discloses a tea tree culture medium and application thereof, and relates to the technical field of biology. The tea tree culture medium comprises a basic culture medium and a microorganism combination, the microbial composition comprises bacillus niger and trichoderma longibrachiatum. The culture medium with air permeability, water-retaining property and fertility is constructed through scientific proportioning, and meanwhile, microorganisms are uniformly colonized in the medium by adding a microorganism combination, so that growth and diffusion of root cancer pathogenic bacteria can be continuously inhibited. Experimental data show that the survival rate of the tea trees cultivated by adopting the tea tree cultivation medium is effectively improved, the root activity is remarkably enhanced, and the dual purposes of disease prevention and control and high quality and high yield of the tea trees are achieved. According to the technical scheme, microbial ecological regulation and control and substrate physical and chemical improvement are combined, and a green and sustainable solution is provided for tea tree planting.

The invention belongs to the field of agricultural planting, and particularly relates to a cultivation method for improving the yield and quality of feeding oats in saline-alkali soil. The invention discloses a cultivation method for improving the yield and quality of feeding oats in saline-alkali soil, which comprises the following steps: in the growth period of oats, drip irrigation is combined with fertilization to improve the yield and quality of oats, the drip irrigation is ground drip irrigation, the interval of drip irrigation belts is 55 cm, the interval of water droppers is 30 cm, irrigation is carried out for five times, and 60 mm of water is irrigated for each time. In the step of fertilization, a biological bacterial fertilizer is applied as a base fertilizer, a water-soluble fertilizer is applied in combination, the water-soluble fertilizer is applied for four times, and the biological bacterial fertilizer is a biological bacterial fertilizer containing plant lactobacillus CICC 10345 and trichoderma longibrachiatum CGMCC 3.6599. Field test results show that the method for improving the yield and quality of the oats in the saline-alkali soil can improve the yield of the oats in the saline-alkali soil and improve the content of crude protein and crude fat in the oats. The invention provides a new thought for healthy development of the saline-alkali soil oat industry.

The present application relates to a strain of Trichoderma longibrachiatum TV26, a microbial agent comprising the same and an application thereof. The strain of Trichoderma longibrachiatum TV26 has a high and persistent antagonistic effect on Pseudomonas lachrymans, and can play a key role in the biological control of cucumber spot disease. Further, through the study of its endogenous metabolites, it is found that the volatile metabolite of turanose as a synergist can significantly improve the colonization ability of the strain on cucumber leaves, so as to be used for developing a more efficient compound microbial agent. Whether the strain of the present application or the compound microbial agent thereof has the characteristics of high efficiency, persistence, safety and environmental protection when used for antagonizing Pseudomonas lachrymans and preventing cucumber spot disease caused by Pseudomonas lachrymans, and therefore has high industrial value and application prospect.

The invention belongs to the technical field of microorganisms, and relates to salt-tolerant trichoderma longibrachiatum and application thereof. The preservation number of the trichoderma longibrachiatum CH-Z1 is CGMCC No. 41752, and the trichoderma longibrachiatum CH-Z1 can improve the salt stress resistance of plants. The trichoderma longibrachiatum CH-Z1 obtained through screening can remarkably inhibit peanut root rot bacteria, peanut black rot bacteria, peanut sclerotium rolfsii and peanut fruit rot bacteria and promote growth of peanut seedlings, is a biocontrol strain with broad-spectrum antibacterial ability and growth promoting effect, and has the advantages of being environmentally friendly, high in specificity, environmentally friendly and the like; and the strain can improve the salt stress resistance of peanut plants and improve the salt-alkali environment, and has important significance for guaranteeing the yield and quality safety of peanuts.

This invention discloses a bamboo vinegar powder extract and its identification method and application. The bamboo vinegar powder extract contains at least one of pyrogallol, 4-methylcatechol, p-hydroxyphenylethanol, p-methylbenzoic acid, dihydrocaffeic acid, and 1,4-dibenzene. Among them, 4-methylcatechol and p-methylbenzoic acid have broad-spectrum antifungal effects, exhibiting very good antifungal activity against Aspergillus terreus CICC40377, Trichoderma longibrachiatum CICC40380, and Paecilomyces variotii CICC40716.

The application discloses a Trichoderma longibrachiatum strain and application thereof in biological control, wherein the strain is Trichoderma longibrachiatum Tr-8, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has a preservation number of CGMCC No. 41721, a preservation date of December 16, 2024, and an address of No. 3, Xili, Beichen West Road, Chaoyang District, Beijing, China, and a telephone number of 010-64807355 of the Institute of Microbiology, Chinese Academy of Sciences. Experiments prove that during the cultivation process, the straw substrate and bacillus subtilis bacteria bodies are compounded to preferentially degrade, and the biological control effect is significantly improved. The relative control effect of the original bacteria on tobacco bacterial wilt is 57.14%, and after the substrate is compounded, the relative control effect is as high as 71.43%. Reasonable optimization of the substrate can significantly enhance the biological control effect of the bacteria on plant pathogenic microorganisms.