126 results about "Trichoderma longibrachiatum" patented technology

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

The invention discloses trichoderma longibrachiatum and application thereof to preventing and treating vegetable diseases. The collection number of the trichoderma longibrachiatum is CGMCC No.4708. The trichoderma longibrachiatum can be applied to preventing and treating vegetable diseases. The trichoderma longibrachiatum Gu 4-5 (or a preparation using the trichoderma longibrachiatum Gu 4-5 as anactive ingredient) is environment-friendly, can inhibit medicament resistance development of disease pathogeny, contributes to popularization of green food and organic agriculture, has low cost and no pollution, and is safe for vegetable crops. Antagonistic microorganisms are introduced into soil, so fungal diseases can be effectively prevented and treated through biological prevention and control, the environment can be effectively improved, an agroecological system can be maintained to be balanced, and strong support is provided for sustainable development.

The invention discloses a biological straw decomposition agent and a preparation method thereof and particularly relates to a biological straw decomposition agent taking funguses and bacteria as raw materials and a production method of the biological straw decomposition agent. According to the biological straw decomposition agent, trichoderma longibrachiatum, aspergillus terreus, phanerochaete chrysosporium, paenibacillus amylolyticus and torulopsis candida are taken as compound stains; a cellulolytic enzyme, a semi-cellulolytic enzyme and a ligninolytic enzyme are mainly produced; the strains have good mutualistic symbiosis and metabiosis effects and have no antagonisms. When the biological straw decomposition agent is used, the decomposition agent is uniformly scattered into fields after crop straws (wheat, corns and rice) are crushed and dispersed on the ground; the dosage of the biological straw decomposition agent is 30kg per 1hm<2> and then the straws and the decomposition agent are ploughed under the ground.

The invention discloses a growing-finishing pig later-stage microorganism fermentation antibiotics-free feed, which is formed by mixing batch and compound fermentation materials. Weight ratio of the batch and the compound fermentation materials is 92-96:4-8, and the compound fermentation materials are formed by compounding the fermentation materials parts by weight, lactobacillus acidophilus fermentation material 2.5-5.5 parts, bacillus subtilis fermentation material 0.05-0.1 parts, saccharomyces cerevisiae fermentation material 0.50-1.85 parts, trichoderma longibrachiatum fermentation material 0.05-0.15 parts, aspergillus niger 0.05-0.2 parts and aspergillus oryzae fermentation material 0.05-0.2 parts. The growing-finishing pig later-stage microorganism fermentation antibiotics-free feedis many in beneficial bacterium variety and large in number and simultaneously contains a plurality of high-activity digestive ferment including beta-dextranase, xylanase, cellulose, protease and thelike, thereby having good synergy function, being capable of improving immunity of organism of animals and improving microecology environment, and further improving digestion using rate of the feed and enhancing economic benefit of cultivation and quality of pork.

The invention discloses a rural domestic garbage and sludge decomposing bacterium agent and a using method as well as a decomposing matrix prepared by using the bacterium agent and a specific process through fermentation. The bacterium agent is compounded by 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum, 0.5-2 parts of aspergillus nige and 0.5-2 parts of bacillus mucilaginosus in parts by weight; the effective viable count of the bacterium agent is 5*10<8>/g; the bacterium agent can be used for decomposing garbage and sludge, deodorizing, resisting fusarium wilt of cucumber and increasing the yield when being applied to crops; the fermentation period of the treated garbage and sludge is 20 days when the bacterium agent is inoculated and 30 days when the bacterium agent is not used. The decomposing matrix is prepared by adding 1 kilogram of bacterium agent into every 5 square mixture through the specific process and fermentation; the mixture is formed by mixing 40-60% of domestic garbage, 20-40% of sludge and 5-15% of corn straws in percentage by weight; when the matrix is applied in cucumber cultivation, the yield is increased by 11% per mu, the income is increased by 859 yuan, and 200mL of pesticide is saved.

The invention discloses a straw composting bacterium agent as well as a preparation method and application thereof and relates to the straw composting bacterium agent. The straw composting bacterium agent is compounded of 5-15 percent of bacillus subtilis powder, 50-70 percent of trichoderma longibrachiatum powder and 25-45 percent of streptomyces griseorubens powder. The preparation method comprises the following steps: respectively preparing zymophytes of bacillus subtilis, trichoderma longibrachiatum and streptomyces griseorubens and products thereof by adopting a solid fermentation method; drying and crushing to form the bacillus subtilis powder, the trichoderma longibrachiatum powder and the streptomyces griseorubens powder; mixing to obtain the straw composting bacterium agent. The straw composting bacterium agent can be applied to preparation of a straw composting and degrading agent.

The invention discloses a livestock and poultry manure decomposing inoculant, a use method thereof and a biological organic fertilizer prepared by using the livestock and poultry manure decomposing inoculant and fermenting through a special process. The livestock and poultry manure decomposing inoculant is prepared by compounding the following materials in parts by weight: 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum and 0.5-2 parts of bacillus mucilagimosus krassilm, wherein the effective viable count is 6*10<8> per gram. The livestock and poultry manure decomposing inoculant disclosed by the invention has multiple functions, can be used for decomposing the livestock and poultry manure and removing the odor and can resist the fusarium oxysporum and increase the yield after being applied to crops. When the livestock and poultry manure decomposing inoculant is applied to treating the livestock and poultry manure, the bacterial count, C/N ratio and fermentation period condition are all better than those of the comparison of an unknown inoculant. The biological organic fertilizer is prepared by adding 1 kilogram of the livestock and poultry manure decomposing inoculant to every 5 squares of the livestock and poultry manure and then fermenting through the special process. The livestock and poultry manure is formed by mixing the following materials in parts by weight: 40-60 parts of chicken manure, 20-40 parts of cattle manure, 5-15 parts of pig manure and 5-15 parts of lignite. The biological organic fertilizer disclosed by the invention is applied to scallion planting, achieves the disease control efficiency up to 56% and increases the yield per mu by 20.5%.

The invention discloses a microbial fermentation antibiotic-free feed for pregnant swine, which is prepared by mixing auxiliary material and compounded fermentation material in a weight ratio of 92-96:4-8, wherein the compounded fermentation material is compounded from the following fermentation materials in parts by weight: 2-5.5 parts of lactobacillus acidophilus fermentation material, 0.05-0.1part of bacillus subtilis fermentation material, 1.00-2.85 parts of Saccharomyces cerevisiae fermentation material, 0.05-0.15 part of trichoderma longibrachiatum fermentation material, 0.05-0.2 part of aspergillus niger fermentation material and 0.05-0.2 part of aspergillus oryzae fermentation material. The microbial fermentation antibiotic-free feed for lean-fleshed pregnant swine contains a large amount of various beneficial bacteria and various of high-activity digestive enzymes such as beta-dextranase, xylanase, cellulose, protease and the like, and has good synergistic effect, can improve the immunity of an animal and intestinal tract microecological environment, and can further enhance the health condition of pregnant swine and the feed digestion and utilization rate; therefore the economic benefit of swine culture is improved.

The invention discloses saline-alkali tolerant trichoderma harzianum and application thereof. The strain number of the saline-alkali tolerant trichoderma harzianum disclosed by the invention is 29HB16; the accession number of the saline-alkali tolerant trichoderma harzianum in a general microorganism center of the China Committee for Culture Collection of Microorganisms is CGMCC No.7975. The trichoderma longibrachiatum 29HB16 disclosed by the invention can tolerate salt stress caused by less than 80 g/L of salinity, and alkali stress of which pH is less than 8.0; the trichoderma harzianum 29HB16 disclosed by the invention is imported to saline alkali soil; a fungal disease can be effectively prevented and treated by biological control; an environment can be effectively improved; balance of an agricultural ecological system is maintained.

The invention relates to a microbial preparation for processing industrial fermentation wastewater. The microbial preparation is prepared by mixing nitrosobacterium, Trichoderma longibrachiatum Rifai, aerobic denitrifying bacterium, acinetobacter baumannii, aspergillus niger, alcaligenes faecalis, Streptomyces lateritius, Candida albicans, Paenibacillus polymyxa and an adsorbent carrier. The microbial preparation contains multiple microbes having excellent ability of degrading non-biodegradable pollutants. Through reasonable compatibility between various strains, the microbial preparation has a good degradating effect and has a wide application prospect.

The invention discloses saline-alkali tolerant trichoderma longibrachiatum and application thereof. The strain number of the saline-alkali tolerant trichoderma longibrachiatum disclosed by the invention is 29TJ04; the accession number of the saline-alkali tolerant trichoderma longibrachiatum in a general microorganism center of the China Committee for Culture Collection of Microorganisms is CGMCC No.7976. The trichoderma longibrachiatum 29TJ04 disclosed by the invention can tolerate salt stress caused by less than 80 g/L of salinity, and alkali stress of which pH is less than pH8.0; the trichoderma longibrachiatum 29TJ04 disclosed by the invention is imported to saline alkali soil; a fungal disease can be effectively prevented and treated by biological control; an environment can be effectively improved; balance of an agricultural ecological system is maintained.

The invention discloses microbial fermentation antibiotic-free feed for a lactating sow. The feed is obtained by mixing a mixture and a prepared fermentation material, wherein the weight ratio of the mixture to the prepared fermentation material is (92-96):(4-8); and the prepared fermentation material is prepared from the following fermentation materials in parts by weight: 2-5.5 parts of lactobacillus acidophilus fermentation material, 0.05-0.1 part of bacillus subtilis fermentation material, 1.00-1.90 parts of saccharomyces cerevisiae fermentation material, 0.05-0.1 part of trichoderma longibrachiatum fermentation material, 0.05-0.2 part of aspergillus niger fermentation material and 0.05-0.2 part of aspergillus oryzae fermentation material. Through the invention, the use of antibiotic drugs in the lactating sow feed can be avoided, the micro ecological environment of the intestinal tract of the lactating sow as well as the immunity are obviously improved, the breeding stress of the sow and the birth stress of the piglets are eased, the problems of constipation and milk lack of the sow are effectively solved, the morbidity is reduced, the feed intake and feed digestion utilization rate are improved, the piglet diarrhea rate is reduced, the survival rate is improved, and the birth weight and weaning weight of the piglets are obviously increased so as to improve the farming benefits.

The invention relates to a microorganism bacterium agent for preventing and treating the ginger basal stem rot and a preparation method and application thereof. The microorganism bacterium agent comprises trichoderma longibrachiatum leavening and a mixed material; the mixed material is formed by humic acid, amino acid and medical stones; a mass ratio of the trichoderma longibrachiatum leavening to the humic acid to the amino acid to the medical stones is (3 to 6):(1 to 2):(1 to 2):(4 to 5); the number of active living bacteria in the trichoderma longibrachiatum leavening is greater than or equal to 2*10<9> per gram. According to the invention, the trichoderma longibrachiatum leavening is applied to prevention and treatment of the ginger basal stem rot; the microorganism bacterium agent for preventing and treating the ginger basal stem rot is prepared; the obtained number of the active living bacteria in trichoderma longibrachiatum is not smaller than 2*10<9> per gram; a prevention and treatment rate for the basal stem rot is high; the morbidity of the ginger basal stem rot can be greatly reduced by not less than 80%; yield of ginger can be improved by not less than 10%; the economic benefits are obvious; quality of the ginger product is improved.

The invention relates to a thorough decomposition agent for locally returning straw to fields, and a preparation method of the thorough decomposition agent, and belongs to the technical field of thorough decomposition agents for straw. The thorough decomposition agent disclosed by the invention has the characteristics of being high in decomposition and disintegration efficiency and short in degradation time, and particularly has favorable disintegration effects at normal temperature and even low temperature. The thorough decomposition agent for locally returning straw to fields, disclosed by the invention, is prepared through the steps of performing fermentation with bacillus amyloliquefaciens, aspergillus niger, trichoderma longibrachiatum and saccharomyces cerevisiae respectively to obtain fermentation liquid, and mixing the fermentation liquid, wherein the ratio of the unit number of colonies of bacillus amyloliquefaciens agent fermentation liquid to the unit number of colonies of aspergillus niger agent fermentation liquid to the unit number of colonies of trichoderma longibrachiatum agent fermentation liquid to the unit number of colonies of saccharomyces cerevisiae fermentation liquid is 2:3:3:1. The thorough decomposition agent for locally returning straw to fields, obtained by the preparation method disclosed by the invention can promote rapid thorough decomposition ofthe straw and enable the thoroughly decomposed straw to return to the fields, can supplement soil organic matter, can sufficiently release and utilize nitrogen, phosphorus and potassium nutrients in the straw, and besides, can improve soil microorganism environment.

The invention relates to the technical field of industrial microorganisms, in particular to a Trichoderma longiflorum, a solid microbial agent thereof and an application thereof. The Trichoderma longibrachiatum (JG9-31, deposit No. CGMCC No. 16280. The invention provides Trichoderma longum JG9-31 can secrete cellulases, promote the rapid maturation of straw, can rapidly propagate in the low temperature range of 10-35 DEG C, and still has a strong ability to degrade cellulose under the low temperature condition, and can rapidly degrade straw. The invention also provides a solid microbial agent,comprising Trichoderma longiflorum JG9-31, Bacillus licheniformis and Streptomyces sp., three kinds of bacteria and the biological enzymes secreted by them form an efficient synergistic system, so that the compound microbial inoculum composed of the three kinds of bacteria can realize the rapid return of straw to the field under the low temperature condition without adding additional cellulase preparation into the compound microbial inoculum.

The invention discloses a sugarcane filtered mud fermentation agent and a sugarcane filtered mud fermentation method. The sugarcane filtered mud fermentation agent comprises bacillus subtilis, aspergillus oryzae, saccharomycetes and trichoderma longibrachiatum, wherein the mass ratio of the bacillus subtilis, the aspergillus oryzae, the saccharomycetes and the trichoderma longibrachiatum is (14-16):(9-11):(4-6):(4-6). According to the sugarcane filtered mud fermentation agent, a plurality of strains are combined at a proper ratio, so that the temperature is raised quickly, the high-temperature time is long, the fermentation time is short and fermentation is conducted completely.

The invention discloses a preparation method of a microbial inoculum for preventing and curing fungal diseases of plants; the microbial inoculum for preventing and curing fungal diseases of plants isprepared by mixing Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum prepared by the method of the invention is used to carry out control efficiency experiments and theresults show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and the control efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by 11.06% so that the effect for preventing diseases and increasing yield is obvious; thecontrol efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

The invention relates to the technical field of microbial agents, and specifically discloses a composite microorganism, a microbial agent containing the composite microorganism and application of themicrobial agent. The composite microorganism includes bacillus licheniformis, trichoderma longibrachiatum, aspergillus niger, bacillus subtilis, bacillus amyloliquefaciens and bacillus pumilus. The provided composite microorganism and its microbial agent have the advantages that the straw decomposition period is short, the degradation rate is high, the consumption is small, the straw turnover timecan be greatly shortened, and the growth of crops is facilitated.

The invention provides a strain of Trichoderma longibrachiatum MF-3, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is April 23, 2018,and the preservation number is CGMCC NO. 15665. The strain has prominent broad-spectrum antibacterial ability, especially has a prominent inhibitory effect on rhizoctonia solani, fusarium oxysporum and fusarium graminearum. The strain can be used for effectively reducing the use of chemical agents in the aspects of inhibiting various soil-borne diseases, and has huge application potential in theaspects of producing pollution-free food and improving ecological environment.

The invention relates to a preparing method of a feed additive, in particular to a preparing method of a pig feed additive. The method includes the steps of mixing jasmine flower residues, wheat bran,rice bran and bean pulp, adding water to be evenly mixed to obtain a solid culture medium, sterilizing the solid culture medium for 30 min at 121 DEG C, cooling the solid culture medium to 28-30 DEGC, inoculating the solid culture medium with a saccharomyces rubrum seed solution and a trichoderma longibrachiatum rifai seed solution, evenly mixing the materials, flat laying the materials on a shallow plate, placing the shallow plate in a manual climatic box to be cultured, conducting low-temperature drying after culturing ends till the water content is 10% or lower, conducting pulverizing through a pulverizer to obtain fermented feed powder, evenly mixing the fermented feed powder, chicken liver powder and feed-grade bile acid, and conducting granulating and packaging to obtain a finishedproduct. The pig feed additive is high in palatability and has effects of promoting pig growth, improving pig immunity and improving pig carcase quality.

The present invention provides a process for producing a tea extract, which comprises adding a protease, a tannase and a cellulase originated from Trichoderma longibrachiatum or Trichoderma reesei to a tea raw material and extracting the desired tea extract from the mixture. According to this process, a tea-leaf-derived cell wall component that cannot be decomposed or extracted by conventional enzymatic tea leaf extraction techniques can be extracted, and a protein of which the extraction becomes possible through the success of the decomposition of the cell wall component can be further decomposed into an amino acid. As a result, it becomes possible to produce a tea extract which contains an amino acid component in abundance and is rich in sweet flavor, robust flavor and "umami" (tasty) flavor in high yield.

The invention relates to a method for performing raw soil greening on coast saline land through a biological modifier containing fly ash. The biological modifier comprises the following components in weight part: 8-12 parts of ceramsite, 11-15 parts of vermiculite, 1-5 part of fly ash, 0.5-2 parts of iron chloride, 0.5-2 parts of calcium dihydrogen phosphate, 0.2-1 parts of formic acid, 1-4 parts of amino acid salt, 2-6 parts of fermented sheep manure, 2-7 parts of capsicum straw powder, 0.3-1 parts of compound sodium nitrophenolate, 0.3-1 parts of amino-oxyacetic acid, 0.3-1 parts of soybean germ extract, 0.5-2 parts of potassium ferrocyanide, 0.2-0.5 parts of cellulose cellulose grafted acrylic acid salt, 0.1-0.5 parts of halomonas, 0.1-0.5 parts of Bacillus subtilis, 0.1-0.5 parts of azotobacter vinelandii, 0.1-0.5 parts of Aspergillus oryzae and 0.1-0.5 parts of Trichoderma longibrachiatum.

The invention relates to the field of pesticides and bacteriostatic agents, in particular to tetranuclear diterpenoids as well as a preparation and an application thereof, wherein the tetranuclear diterpenoids are sources of seaweed endophytic fungi. The concrete structural formula of the tetranuclear diterpenoids is shown in formula (I), and a preparation method of the tetranuclear diterpenoids comprises the steps as follows: (Trichoderma longibrachiatum)cf-11 is inoculated in fugus fluid nutrient medium for fermentation cultivation; in addition, fermentation products are purified, so as to obtain the tetranuclear diterpenoids shown in formula (I). Pesticidal activity experiments prove that the crustacean lethality is 82.6 percent when the obtained tetranuclear diterpenoids are 100 micrograms per milliliter, and the crustacean lethality is 50 percent when the concentration of the obtained tetranuclear diterpenoids reaches 23.1 micrograms per milliliter; and in addition, the tetranuclear diterpenoids also achieve bacteriostatic activity.