235 results about "Microbial composition" patented technology

The present disclosure relates to the identification of a group of microorganisms, which are relatively abundant in the microbial communities associated with fruits and vegetables typically consumed raw and therefore transient or permanent members of the human microbiota. These microbes are used to augment the effects of additional probiotic strains. The consumption of mixtures of these microbes at relevant doses will produce a beneficial effect in the host. Therapeutic methods of the disclosure involve the use of live microorganisms or metabolites derived from said microorganisms to establish a microbial composition in the mammalian host that will provide a health benefit to a mammal in need thereof.

Provided are a quality-improving and nitrogen-fixing coupled microbial agent and the use thereof. The quality-improving and nitrogen-fixing coupled microbial agent is a microbial composition, has coupled functions of improving quality and fixing nitrogen so as to improve the quality of products of leguminous crops, and has the functions of regulating and increasing the rhizobial abundance for the rhizosphere of leguminous crops and increasing the amount of nodules of leguminous crops. The microbial agent contains all gene sequences of nucleotide sequences shown as SEQ ID NO.1-12 or four or more gene sequences among same. When being applied to production of leguminous crops, such as soybeans, peanuts, peas, broad beans and cowpeas, the quality-improving and nitrogen-fixing coupled microbial agent has coupled functions of improving quality and fixing nitrogen and achieves quality improvement and nitrogen fixation and green yield increase. The microbial agent is easy to use, achieves remarkable social and ecological benefits, and facilitates popularization and use.

The invention belongs to the technical field of microbial agents, and particularly relates to a preservative and fresh-keeping microbial composition as well as a microbial agent and application thereof. The microbial composition disclosed by the invention comprises bacillus subtilis LS and bacillus velezensis LX, and in the microbial composition, the concentration ratio of the bacillus subtilis LS to the bacillus velezensis LX is (0.1-1): (0.1-1); the preservation number of the bacillus subtilis LS is CGMCC (China General Microbiological Culture Collection Center) NO.30953; the preservation number of the bacillus velezensis LX is CGMCC (China General Microbiological Culture Collection Center) NO. 30954. In the microbial composition provided by the invention, the bacillus subtilis LS and the bacillus velezensis LX can generate a synergistic effect and can be used for preventing and treating the soft rot of the kiwi fruits, and a theoretical basis and technical guidance are provided for biological prevention and treatment and storage and fresh-keeping of the soft rot of the kiwi fruits.

The invention discloses a soybean phospholipid disease-preventing and root-promoting microbial agent as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises a microbial composition, modified enzymolysis soybean phospholipid, a carrier and auxiliary components. According to the invention, the modified enzymolysis soybean phospholipid and microorganisms are combined to achieve a synergistic effect. According to the method, soybean phospholipid is modified and subjected to enzymolysis, and the steps of pretreatment, stepped low-temperature treatment, enzymolysis treatment, enzyme inactivation and purification and the like are carried out, so that the quality and performance of the soybean phospholipid are improved, the rhizosphere colonization rate of strains can be increased, the disease prevention and root promotion effects can be improved, the soil micro-ecological environment can be improved, and good growth conditions are created for plant root systems; and the disease-preventing and root-promoting effects of the microbial agent are further enhanced. The microbial agent disclosed by the invention can be used for remarkably reducing the morbidity of wheat sharp eyespot and reducing the use amount of chemical pesticides, can also be applied to planting of other crops, and can play a role in preventing and controlling various plant diseases and promoting root growth.

The present invention relates to a microbial combination and a fermented milk and the use thereof, in particular to a multi-strain microbial combination, a fermented milk prepared from the microbial combination and the use of the microbial combination in the fermentation of a camel milk. The microbial composition contains at least two of lactic acid bacteria and yeasts derived from traditional fermented milk products in Xinjiang, including Leuconostoc pseudomesenteroides, Lactobacillus rhamnosus, Debaryomyces hansenii and Kluyveromyces marxianus. The microbial composition ensures that industrially fermented camel milk products have the flavor and nutritional functions of naturally fermented sour camel milk, but with higher quality, better safety and significantly prolonged shelf life. The microbial composition has relatively high acidification efficiency, proteolysis capacity and lactose decomposition capability, can improve the unpleasant flavor of camel milk products industrially fermented by using conventional strains, and has wide application prospects in industrial production.

The invention relates to the technical field of microorganisms, in particular to a complex microbial inoculant for resisting salmonella infection and application of the complex microbial inoculant. The invention provides a microbial composition for resisting salmonella infection. The microbial composition comprises lactobacillus reuteri, lactobacillus mucosa, lactobacillus plantarum, lactobacillus bulgaricus and pediococcus pentosaceus. Results of the embodiment of the invention show that the complex microbial inoculant provided by the invention has an excellent anti-salmonella typhimurium effect, and only the anti-salmonella typhimurium effect of the complex microbial inoculant in a specific proportion provided by the invention is optimal; meanwhile, the complex microbial inoculant can effectively relieve the harm of salmonella typhimurium to chickens, improve the growth performance of the chickens and improve the immunity of the chickens. The invention provides a new prevention and treatment thought for salmonella.

The invention discloses a feed additive for improving mutton quality and producing low-mutton-smell mutton, which is characterized in that through combined application of phytosterol and dl-alpha-tocopheryl acetate, the microbial composition of the lamb intestinal tract is improved, especially the abundance of beneficial bacteria is improved, and the abundance of potential pathogenic bacteria is reduced. The combined use of the two can improve the intestinal microbial ecology, and significantly reduce the drip loss, shear force and Plt of mutton; 0.05, the mutton color and the tenderness of the mutton are improved; excrement causing secondary pollution to the environment is not generated. The application of the plant extract in the production of the low-mutton-smell mutton is reported for the first time, the mutton smell can be remarkably reduced by combining and adding the phytosterol and the dl-alpha-tocopheryl acetate according to a certain proportion, and meanwhile the mutton quality is improved. The feed additive disclosed by the invention is simple in components, remarkable in effect and simple and convenient to apply, and has great potential popularization and application values.

The invention relates to the technical field of wastewater treatment, in particular to a method for constructing a quick-response accurate evaluation model for the stability of a wastewater biochemical system. The method comprises the following steps: acquiring an activated sludge image sample at the tail end of an aeration tank and effluent quality information of a sedimentation tank; generating microorganism composition information in the aeration tank activated sludge according to the aeration tank activated sludge image sample; generating biochemical system rating information matched with the activated sludge composition information according to the effluent quality information of the sedimentation tank, the microorganism composition information in the activated sludge of the aeration tank and a preset water quality evaluation system; and repeatedly acquiring the active sludge composition information and the biochemical system rating information, and storing the active sludge composition information and the biochemical system rating information generated each time to generate the rapid response wastewater biochemical system stability accurate evaluation model. The invention provides an evaluation model which can be quickly obtained, is high in timeliness, is not interfered by human factors, and is high in accuracy and reliability for the field of wastewater treatment.

The invention belongs to the technical field of microorganisms, and particularly relates to a caproic acid-producing microbial composition and application thereof. According to the specific technical scheme, the microbial composition comprises caproic acid bacteria, clostridium and lactic acid bacteria. The caproic acid producing microbiome provided by the invention not only can be used for resourceful treatment of wastewater with high lactic acid concentration and reduction of environmental protection cost, but also can be used for construction and maintenance of pit mud, and provides a new practical reference for research of Luzhou-flavor liquor synthesis microbiome and preparation of artificial pit mud.

The present invention relates, in general terms, to a face mask. The present invention also relates, in general terms, to composite material, iron-iron oxide compositions and their methods of fabrication thereof. In particular, the iron-iron oxide composition comprises a cashew testa extract and iron particles and / or oxide core-shell particles. The iron-iron oxide composition can be used as an anti-microbial composition.

The present invention relates to the field of microorganisms and also to the field of crop fertilizers. Discloses is a use of an ARC microbial agent in the production of an ARC+ fertilizer. The ARC microbial agent is a microbial composition, has a coupled effect of toxicity control and nitrogen fixation and the effects of regulating and controlling the abundance of rhizobia in the rhizosphere of a leguminous crop and increasing the quantity of nodules in the leguminous crop, and contains DNA sequences as shown in SEQ ID Nos. 1-4. The fertilizer is a complex fertilizer / compound fertilizer, an organic fertilizer, or a microbial agent. Further disclosed are a method for producing an ARC+ fertilizer by compounding an ARC microbial agent with a fertilizer, an ARC+ fertilizer prepared by the method, and a use of the ARC+ fertilizer in crop production. The ARC+ fertilizer can act as both an ARC microbial agent and a fertilizer, and the operation step of mixing the two by a user can be removed, thereby avoiding the problem of non-uniform application of the ARC microbial agent.

The invention relates to the technical field of fertilizers, and particularly discloses a cold-resistant fruit and vegetable fertilizer and a preparation method thereof.The cold-resistant fruit and vegetable fertilizer is prepared from, by mass, 55-80 parts of a compound fertilizer, 10-30 parts of a chitosan-based biomass charcoal composite material, 3-5 parts of a microbial composition and 1-3 parts of a plant growth regulator; wherein the biomass charcoal is obtained by carbonizing seaweed, wheat straw and peanut shells as raw materials, the chitosan-based biomass charcoal composite material has a porous structure, has excellent adsorption and slow release effects and good fertilizer and water retention effects, most of the raw materials in the fertilizer are natural organic substances, pollution to soil and the environment is avoided, and under low-temperature stress, the fertilizer has good fertilizer and water retention effects. The fertilizer protects plants through multiple mechanisms, can continuously provide nutrients, guarantees the yield and quality of fruits and vegetables, can adapt to different fruit and vegetable varieties and low-temperature climate conditions in different regions by adjusting the proportion of all the components, and is wide in applicability.

This invention relates to the area of microorganism identification, specifically involving a method of obtaining microorganism identities and related information by sequencing. The method includes: i) obtaining sequencing data, said sequencing data are obtained by amplification of microbial characteristic sequences using primers followed by sequencing the amplification products using next-generation sequencing technology; ii) comparing said sequencing data with characteristic sequence database to identify microbial composition in said samples tested; wherein perform clustering on said characteristic sequence database in advance based on the sequence similarity among reference sequences containing said characteristic sequences, obtain one or more tiers of clusters, there is at least one child seed in each cluster, and there are several seeds as reference sequences in the bottom tier cluster.

The disclosure provides microbial composition and application in reducing biogenic amines, and belongs to the technical field of food processing and manufacturing. The disclosure provides a construction method for an inoculated biogenic amine degrading lactic acid bacteria flora and an application in reducing biogenic amines in cyclic rice steeping and persistently inhibiting invasion of amine producing microorganisms. In the disclosure, a lactic acid bacteria ternary interacting flora that efficiently inhibits invasion of the amine producing microorganisms is constructed. In a rice steeping process in preparation of a brewed product, the lactic acid bacteria ternary interacting flora is inoculated for rice steeping, and a cyclic rice steeping process is improved. The result shows that inoculation of the lactic acid bacteria ternary interacting flora and utilization of the improved rice steeping process may effectively reduce the content of biogenic amines in rice steeping water and completely inhibit invasion of the amine producing microorganisms to realize zero growth of the biogenic amines, thereby creating conditions for the cyclic rice steeping process in mass production in a plant.

The invention discloses application of a microbial composition in industrial hazardous waste treatment, and belongs to the technical field of industrial hazardous waste treatment.The application comprises the following steps that S1, industrial hazardous waste is collected, the industrial hazardous waste is pretreated to obtain a hazardous waste material, and a fermentation tank is filled with the hazardous waste material; s2, adding a growth culture medium into a bioreactor, inoculating the domesticated bacterial liquid into the bioreactor, and carrying out enlarged culture to obtain a fermentation material of the composite acidophilic thiobacillus; s3, adding a biosurfactant into the fermentation material of the composite acidophilic thiobacillus, and uniformly stirring to obtain the composite acidophilic thiobacillus with hydrophobically modified cell membrane surface; and S4, adding an acidic pH buffer solution to obtain a to-be-treated material. According to the method, in the industrial hazardous waste treatment process, the stability of the pH required by the acidithiobacillus acidophilus can be maintained, and the cell membrane surface hydrophobicity of the composite acidithiobacillus acidophilus is improved.

Provided herein are compositions, methods, kits, and devices for the treatment of skin diseases. Also provided herein are isolated and purified bacteria, excipients, carriers, dosage forms and routes of administration for such bacteria. Furthermore, provided herein are isolated and purified mixtures of bacteria, excipients, carriers, dosage forms and routes of administration for such mixtures. Also provided herein are conditions for treatment with bacteria and bacterial mixtures.

The invention relates to a microbial biofilm cultivation method comprising the use of a soft agar hydrogel substrate and one or more stains for detection and / or quantification of one or more microbial components and / or characteristics. The invention further relates to the use of the microbial biofilm cultivation method for screening of one or more antimicrobial compounds.

The present invention relates to a method for producing a stable hydrogel-cell composition comprising the steps of:a) providing a reaction mixture comprising at least one polymerizable substrate and at least one enzyme capable of polymerizing said at least one substrate, and cells, andb) incubating the mixture of step a) to form a stable hydrogel-cell composition.

The present invention relates to microbial compositions, useful as biological amendments to enhance plant growth. Provided are isolated microbial strains, agricultural compositions comprising them, and their use in methods of improving plant growth and / or increasing plant yield.

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.

The invention discloses a microbial composition for synergistically degrading atrazine and 2, 4-D and a microbial agent produced by the microbial composition. The invention relates to a microbial composition, which is prepared from Arthrobacter sp. AT5 with the preservation number of Arthrobacter sp. AT5, Sphingobium sp. L3 with the preservation number of Sphingobium sp. L3 and Bacillus sp. R20C with the preservation number of Bacillus sp. The microbial composition disclosed by the invention and the microbial agent produced by the microbial composition can degrade two pollutants, namely atrazine and 2, 4-D. The problem of pollution caused by triazine and phenoxy carboxylic acid herbicides widely used in global agricultural production is successfully solved, so that the ecological environment is protected, and human health is maintained.

The invention provides a method for rapidly detecting microbial species, and particularly, the method comprises the following steps: constructing a double-level database and a corresponding gene cluster; constructing a reference matrix X based on the database and the gene cluster; comparing to-be-detected third-generation sequencing data with the gene cluster to generate equivalence classes, and constructing a counting matrix Y based on the equivalence classes and the gene cluster; based on the counting matrix Y and the reference matrix X, determining the gene abundance in the to-be-detected sample; and determining microbial species in the sample to be detected based on the gene abundance. The rapid pathogen detection method provided by the invention is high in analysis speed, fully utilizes the advantages of the three-generation sequencing data, and can more directly obtain the microorganism composition information of the sample; the method is low in computing resource demand, easy to deploy and high in precision detection capability.

Compositions and methods for converting at least one polyphenol to protocatechuic acid (PCA) using Bacillus subtilis 1579, or active variants thereof, are provided. Conversion of polyphenols, such as quercetin, to PCA can decrease inflammation, decrease cortisol levels, and increase milk quality and milk production quantity in milk-producing agricultural animals. Accordingly, provided herein are compositions comprising B. subtilis 1579, or an active variant thereof, for administration to humans for decreasing inflammation or for administration to milk-producing agricultural animals for decreasing inflammation, decreasing cortisol levels, and increasing milk quality and milk production.

The invention provides a sisal hemp residue vegetable seedling organic fertilizer and a preparation method and application thereof. The sisal hemp residue vegetable seedling organic fertilizer comprises a first fertilizer bag, the first fertilizer bag is prepared by fermenting the following raw materials in parts by weight through a microbial composition: 90-150 parts of fresh sisal residues, 5-20 parts of sugarcane leaves, 5-20 parts of an organic nitrogen source, 2-5 parts of molasses, 0.5-1 part of monopotassium phosphate, 0.05-0.2 part of magnesium sulfate and 0.3-0.8 part of calcium superphosphate; the sisal hemp residues and other raw materials are compounded for vegetable seedling raising, nutrients are rich, the vegetable planting benefits are improved, and the planting cost is reduced.

The invention relates to the technical field of microbial compositions, in particular to a microbial composition for preventing and treating citrus canker and application of the microbial composition. According to the microbial composition provided by the invention, the pseudomonas Qingdao microbial agent, sodium lignin sulfonate, trehalose, glutathione, ascorbic acid, lecithin and trace elements (Zn, B and Mn) are taken as main raw materials, and the effects of efficiently inhibiting bacteria, remarkably inducing and improving the activity of key defensive enzymes of leaves, promoting synthesis and accumulation of disease-resistant substances and improving the disease resistance of the leaves are achieved through the matching effect of all the components. The diversity and richness of citrus phyllospheric bacterial communities are improved, and the purpose of constructing a stable community dominated by beneficial bacteria is achieved. In other words, the basic stress resistance of the plants can be enhanced, and meanwhile, the growth and metabolism activity of beneficial bacteria is also promoted, so that effective, eco-friendly and sustainable prevention and control of the citrus canker are achieved.

The invention provides yellow-rose yellow-meat boletus fucomannan as well as a composition, a preparation method and application thereof, and belongs to the technical field of biological medicine mass health. The fucomannan has a structure represented by a general formula in an attached drawing of the abstract, and n represents the number of sugar repeating units and is a natural number. The weight-average molecular weight of the fucose glucomannan galactan of the boletus fucose is 10 kDa to 20 kDa, the polydispersity coefficient of the fucose glucomannan galactan is 1 to 2, and the molar ratio of glucose to mannose to fucose to galactose is 1.00 to 3.08 to 2.84 to 14.86. The boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan have the effects of promoting proliferation of beneficial bacteria, reducing the pH value of a microenvironment, increasing secretion of SCFAs and lactic acid, adjusting composition of intestinal microorganisms and the like, and have the function of improving the intestinal microecology. Therefore, the invention also discloses the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the composition of the composition of the composition of the composition of the composition of the composition of

The present invention relates to compositions comprising disintegrated microbial biomass and / or microbial biomass fractions in combination with other ingredients, and methods of producing such compositions. The invention further relates to the use of these composition in commercial products such as food products.

Disclosed herein is a strain of Bacillus infantis deposited under number CECT 30759. Also, a microbial composition and process for the preparation thereof. Particularly, a microbial composition based on Bacillus infantis strain used as plant growth promotion bacteria (PGPB), more specifically as a biological fixing nitrogen. In addition, the present invention discloses a method for reducing the total amount of nitrogen fertilizer required by crop.

The present invention is directed to a surfactant preparation and end use product comprising alcohol alkoxy sulfates where the preparation and composition display mitigation in dioxane formation when antimicrobial agent is present in the surfactant preparation and end use product.