This invention pertains to surface-enhanced
Raman scattering (SERS) detection technology, specifically disclosing a
signal-squeezing SERS detection method for achieving quantitative analysis over an ultra-wide concentration range. It utilizes two-dimensional
transition metal phosphorus-
sulfur compounds with structural differences as the SERS substrate, modifying them through mechanical strain and
chemical modification to enhance their sensitivity to specific and continuous concentration ranges. For each substrate, three parameters—characteristic
peak intensity,
signal-to-
noise ratio (SNR), and detection probability—are extracted to construct a three-parameter calibration
radar map for the corresponding concentration range. During detection, the three-parameter vector of the
analyte at an unknown concentration is projected onto the three-parameter calibration
radar map. Using the
signal "squeezing" principle, the concentration level is determined by the triangular region formed by the
analyte and the known concentration vector. This invention overcomes the
bottleneck of narrow concentration range detection with a single SERS substrate, achieving a detection range of 10... ‑6 -10 ‑22 M offers precise quantitative detection over an ultra-wide concentration range, is easy to operate, and provides reliable results, making it a promising candidate for applications in fields such as biosensing, medical diagnostics, and
environmental analysis.