Sphingomonas strain and application thereof in water treatment

A sphingomonas and strain technology, applied in the fields of environmental engineering and bioengineering, can solve problems such as harsh control conditions and limited application, and achieve the effects of high biosafety, broad application potential and strong degradation ability.

Inactive Publication Date: 2011-08-31
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods also have the disadvantages of harsh control conditions and easy conversion to full nitrification, which limits their application.

Method used

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  • Sphingomonas strain and application thereof in water treatment
  • Sphingomonas strain and application thereof in water treatment
  • Sphingomonas strain and application thereof in water treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 The screening steps of the Sphingomonas bacterial strain provided by the present invention:

[0039] Use a pre-sterilized layered water sample collector to collect 0.25 L of water samples at different depths above the sediment interface of Taihu Lake, and pack them in pre-sterilized brown glass bottles; use a pre-sterilized Peterson mud sampler (1 / 16m 2 ) to collect the surface sediments of Taihu Lake (thickness is about 10cm), put them into a sterilized stainless steel container; Brown glass bottles, sealed immediately after sampling the above samples, used as bacteria source for isolation and enrichment culture. Under sterile conditions, add the water sample to the ammonia oxidizing bacteria enrichment medium (the composition of the ammonia oxidizing bacteria medium is (NH 4 ) 2 SO 4 0.2g, K 2 HPO 4 0.1g, MgSO 4 0.05g, NaCl 0.2g, FeSO 4 0.04g, CaCO 3 0.5g, H 2 O 100ml, pH value 7.2, wherein the culture medium was used after sterilizing wit...

Embodiment 2

[0048] The application of the present invention to the research on the degradation of ammonia nitrogen in the rich nutrient medium, its steps are as follows:

[0049] [1] Add 50ml of rich nutrient medium (ammonia nitrogen concentration is 100mg / L) into a 250ml Erlenmeyer flask.

[0050] [2] Add the cell liquid culture of the Sphingomonas strain prepared in Example 1 into the Erlenmeyer flask of the above [1], and cultivate it aerobically at 28°C and 80r / min. Samples were taken every 2 hours. figure 1 Degradation of ammonia nitrogen by Sphingomonas strains in enriched medium. from figure 1 It can be seen that a small amount of bacterial growth is enough to degrade ammonia nitrogen, and the removal rate of ammonia nitrogen is close to 100% after 6 hours of inoculation.

Embodiment 3

[0052] The present invention uses glucose and CO 2 For the application of nitrite degradation research in mixed carbon source and nitrite as nitrogen source medium, the steps are as follows:

[0053] [1] The medium in Example 1 was replaced with glucose and CO 2 It is a mixed carbon source, nitrite is the basal medium of nitrogen source (nitrite concentration is 100mg / L)

[0054] [2] Add the cell liquid culture of the Sphingomonas strain prepared in Example 1 into the Erlenmeyer flask of the above [1], and culture it anaerobically at 28°C, 80r / min. Samples were taken every 2 hours. figure 2 Degradation of nitrite by Sphingomonas strains in enriched medium. from figure 2 It can be seen that a small amount of bacterial growth is enough to degrade nitrite, and after 12 hours of inoculation, the removal rate of nitrite is close to 100%.

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Abstract

The invention belongs to the technical fields of environmental engineering and bioengineering and particularly relates to a sphingomonas strain and applications thereof in the aspects of shortcut nitrification-denitrification of nitrogen-containing industrial waste water and domestic sewage and treatment of a polluted water source. The sphingomonas strain is separated from a Taihu water in China,is a local strain and has high safety; and the strain can be grown in a basic medium, wherein in the basic medium, CO2 is used as a carbon source and energy or CO2 and an organism are used as a mixedcarbon source and energy, and ammonia nitrogen or nitrate nitrogen is used as a nitrogen source. The bacterium liquid, the dormancy cell and the immobilized strain of the sphingomonas can decompose ammonia nitrogen into nitrite nitrogen and simultaneously can decompose the ammonia nitrogen into nitrogen, can be used as denitrification microorganism for converting the ammonia nitrogen into the nitrite nitrogen and the nitrogen, thereby achieving the shortcut nitrification-denitrification. The strain can rapidly decompose the ammonia nitrogen and is suitable for treating the nitrogen-containingindustrial waste water and domestic sewage as well as polluted water source.

Description

technical field [0001] The invention belongs to the technical fields of environmental engineering and biological engineering. It specifically relates to the strain of Sphingomonas and its short-range nitrification-denitrification denitrification for nitrogen-containing industrial wastewater and domestic sewage, and its application in the treatment of polluted water source water. Background technique [0002] In recent years, with the rapid development of industrial and agricultural production and the progress of society, the pollutants discharged into water bodies have increased significantly, and the discharge of nitrogen-containing wastewater has also increased sharply, leading to eutrophication in major rivers, five major freshwater lakes and coastal waters in my country. Algal blooms occur frequently. The ammonia nitrogen entering the water body is oxidized into nitrite and nitrate, which consumes a large amount of oxygen, reduces the quality of the water body, and serio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 李正魁周莉王月明潘静赟王易超范念文陈祈春吴凯冯露露吴宁梅赵琳周涛
Owner NANJING UNIV
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