65 results about "Ammonia-oxidizing bacteria" patented technology

The invention relates to a soil conditioner based on nitrification inhibition and a soil acid control method, and belongs to the technical field of soil conditioning, the soil conditioner comprises the following components by weight: 20-22 parts of humic acid, 32-36 parts of syringic acid-linoleic acid modified porous hydroxyapatite, 4-5 parts of complete nitrifying bacteria, 2-5 parts of syringic acid, and 2.8-4.2 parts of sodium carboxymethyl cellulose. According to the technical scheme, syringic acid and linoleic acid serve as nitrification inhibitors, the nitrification inhibition rate can be effectively increased, ecological niches of ammonia-oxidizing archaea and ammonia-oxidizing bacteria can be reduced through competitive occupation due to addition of complete nitrifying bacteria, the nitrification rate of the ammonia-oxidizing archaea and the ammonia-oxidizing bacteria is inhibited, and N2O emission is reduced. Humic acid and hydroxyapatite can be used as pH buffer substances to regulate and control soil acidity, aluminum toxicity can be relieved, and physical and chemical properties of soil can be improved; meanwhile, the complete nitrifying bacteria can be ensured to have relatively high activity under the condition that the pH is 5.5-6.5.

The invention relates to the technical field of wastewater treatment or water quality purification, in particular to a method for synchronously culturing ammonia oxidizing bacteria and nitrite oxidizing bacteria, which comprises the following steps: selecting an inorganic material which is large in specific surface area and can quickly neutralize and release alkalinity as a nitrifying bacteria attachment carrier and an alkalinity supplement / pH regulator; the carrier is dissolved through nitration acid production, carbon dioxide and alkalinity are released, a carbon source and alkalinity required by growth are provided for nitrifying bacteria, and propagation of the nitrifying bacteria is promoted. Ammonia oxidizing bacteria and nitrite oxidizing bacteria are cultured synchronously by controlling the growth speed of the two bacteria in the culture process. By controlling the ammonia nitrogen concentration and nitrite concentration of the effluent at the end of culture to be lower than 50mg / L, sufficient time is provided for nitrite oxidizing bacteria to decompose an intermediate product nitrite, so that the purpose of simultaneously culturing AOB and NOB is achieved.

The invention discloses a method for realizing deep autotrophic nitrogen removal of sludge digestion liquid based on alternate intermittent aeration of a double-membrane aeration bio-membrane reactor (MABR). The process device comprises a sludge digestion liquid raw water tank, a single-stage double-MABR reactor, two MABR devices and a water outlet tank. According to the method, sludge digestion liquid enters the single-stage double-MABR reactor, the two MABR devices are alternately and intermittently aerated, the state of one aeration and one aeration stopping is always kept, and ammonia nitrogen in raw water is converted into nitrite through ammonia oxidizing bacteria enriched on the inner side of an MABR membrane. And simultaneously supplying to anaerobic ammonium oxidation bacteria enriched on the outer sides of the two MABR membranes through matrix diffusion for denitrification and generation of nitrate. Denitrifying bacteria enriched on the outer side of the MABR membrane utilize raw water organic matters to drive short-cut denitrification to synchronously supply nitrite to anaerobic ammonium oxidation bacteria so as to realize deep autotrophic nitrogen removal. According to the invention, a double-anaerobic ammonia oxidation reaction hot area is constructed through alternate intermittent aeration of double MABRs, and efficient deep autotrophic nitrogen removal of the sludge digestion liquid can be stably realized.

This invention relates to an optimized control method for the enrichment of anaerobic ammonia-oxidizing bacteria based on niche regulation, comprising: S1, selecting key niche indicators of constructed wetlands as investigation factors, differentially setting the investigation factors to form experimental systems with different niches, conducting constructed wetland control and monitoring, and collecting data; S2, using the relative abundance and / or absolute abundance of anaerobic ammonia-oxidizing bacteria collected in S1 as dependent variables and the key niche indicators as independent variables, fitting and then predicting a model; S3, making predictions using the prediction model, comparing the prediction results with the control target, and optimizing the control of anaerobic ammonia-oxidizing bacteria enrichment in constructed wetlands based on the comparison results. This method can obtain a prediction model with high prediction accuracy, and then, by adjusting the niche indicators in constructed wetlands, the enrichment of anaerobic ammonia-oxidizing bacteria can be ensured, wastewater treatment efficiency can be improved, and a better total nitrogen removal effect can be achieved.

This invention discloses a continuous-flow anaerobic bypass IFAS-AOA device and method for treating inert high-ammonia nitrogen wastewater, belonging to the field of biological treatment technology for high-ammonia nitrogen wastewater. The device consists of three parts: a raw water storage tank, a continuous-flow anaerobic bypass AOA reactor, and a sludge-water separation system. The effluent from the end of the anaerobic process enters the aerobic zone of the AOA reactor, while the anaerobic end-mixed liquor bypasses to the anoxic zone, mixes with the aerobic end-effluent, flows through the anoxic zone, and enters the sludge-water separation system. In the anaerobic zone, endogenous denitrifying bacteria store organic matter from the raw water as an intracellular carbon source and bypass to the anoxic zone. In the aerobic zone, short-cut nitrification coupled with anaerobic ammonia oxidation serves as the main nitrogen removal pathway. In the anoxic zone, anaerobic ammonia oxidation coupled with endogenous denitrification achieves deep nitrogen removal. The existence of anaerobic bypass allows some of the mixed liquor to bypass the aerobic zone and directly bypass to the anoxic zone, thus fully utilizing the carbon source in the raw water. This invention can achieve stable short-cut nitrification and effective retention of anaerobic ammonia oxidizing bacteria, showing broad application prospects.

The invention relates to the technical field of soil conditioners and applications thereof, and discloses a soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum and an application thereof in ploughing ability improvement, the soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum comprises an organic pollutant degradation functional flora, a soil carbon sequestration functional flora and a small molecular organic matter production functional flora; the organic pollutant degrading functional flora comprises efficient pollutant degrading bacteria; the soil carbon sequestration functional flora comprises ammonia oxidizing bacteria, ammonia oxidizing archaea, complete ammonia oxidizing bacteria, sulfate reducing bacteria, iron reducing bacteria, rhizobium and photosynthetic flora; the micromolecular organic matter production functional flora comprises heterotrophic nitrification-aerobic denitrification bacteria, zoogloea, phosphorus-accumulating bacteria and photosynthetic bacteria. According to the scheme, multiple florae are specifically arranged in the synergistic agent, and through the synergistic interaction effect of the florae, the effects of the synergistic agent in multiple fields of soil carbon sequestration, pollution reduction and yield increase are effectively improved.

The invention discloses a starting and control method for treating ammonia-nitrogen wastewater by an integrated complete ammoxidation coupling short-cut denitrification anaerobic ammoxidation process, and belongs to the field of wastewater biological treatment. The starting method adopts a step-by-step inoculation strategy, and comprises the following steps: firstly, inoculating complete ammoxidation sludge in the SBR reactor for low-oxygen domestication, and after successful starting, supplementing and inoculating short-cut denitrification coupled anaerobic ammoxidation sludge. The control method adopts a sectional water inlet mode, and comprises the following steps: a first part of ammonia nitrogen wastewater firstly enters an integrated SBR (Sequencing Batch Reactor), and ammonia nitrogen is directly oxidized into nitrate nitrogen through complete ammonia oxidizing bacteria (Comammox) under the condition of low dissolved oxygen; aeration is stopped, a carbon source is added for short-cut denitrification, and nitrate nitrogen is reduced into nitrite nitrogen; and then pumping a second part of ammonia nitrogen wastewater, and converting ammonia nitrogen in the wastewater and accumulated nitrite nitrogen into nitrogen by using anaerobic ammonium oxidation bacteria, so as to remove the ammonia nitrogen in the wastewater. According to the invention, deep denitrification is realized under the condition of saving energy consumption and a carbon source.

The present disclosure provides a sewage treatment method and an execution system, comprising: under the conditions of stirring and anaerobic, absorbing and releasing phosphate by anaerobic phosphorus release and internal carbon source conversion reaction in the first sewage, taking the carbon-containing organic matter in the first sewage as a carbon source, to obtain the second sewage; performing aerobic treatment on the second sewage, determining the relative activity of aerobic phosphorus accumulating bacteria and aerobic ammonia-oxidizing bacteria in the second sewage according to the pH value change trend of the second sewage; treating the second sewage by using a multi-stage aeration mode according to the relative activity, adjusting the dissolved oxygen concentration by adjusting the aeration mode of the multi-stage aeration, to occur integrated nitritation-anammox and aerobic phosphorus absorption reaction, to obtain the third sewage; under the conditions of stirring and anaerobic, performing deep phosphorus and nitrogen removal on the third sewage by anaerobic ammonia oxidation reaction, denitrification phosphorus removal reaction and internal carbon source denitrification reaction to obtain the fourth sewage; standing the fourth sewage to separate the sludge and water, and outputting the sewage obtained after the sludge and water separation.

This invention relates to an optimized control method for total nitrogen removal and anaerobic ammonia-oxidizing bacteria enrichment in constructed wetlands, comprising: S1, selecting control factors for the constructed wetland, forming an experimental system using a central coincidence design method, introducing wastewater to be treated into the constructed wetland, monitoring the operation of the constructed wetland, and collecting data on total nitrogen removal and relative abundance of bacteria; S2, using analysis of variance to determine a suitable model method for data fitting, and then constructing prediction models for total nitrogen removal efficiency and relative abundance of anaerobic ammonia-oxidizing bacteria based on the model method; S3, predicting the total nitrogen removal efficiency and relative abundance of anaerobic ammonia-oxidizing bacteria in the constructed wetland, thereby achieving optimized control of the operation and maintenance management of the constructed wetland. The optimized control method provided by this invention can predict the relative abundance of anaerobic ammonia-oxidizing bacteria and the total nitrogen removal efficiency of constructed wetlands, guiding the operation and maintenance management of constructed wetlands.

The invention provides a two-stage HRBPR-PN / A process and device based on sludge biological enhancement. In the front section of the process, an endogenous carbon source is taken in and phosphate is released in an anaerobic stage through phosphorus-accumulating bacteria (PAOs), and then excessive phosphorus is absorbed in an aerobic stage. And the rear stage is an anaerobic / aerobic alternate operation short-cut nitrification anaerobic ammonia oxidation (PNA) process, under the condition of low dissolved oxygen, ammonia oxidizing bacteria AOB and anaerobic ammonia oxidizing bacteria AnAOB cooperate to efficiently remove nitrogen, and meanwhile, residual organic matters in the previous stage and nitrate in the system are removed by strengthening endogenous denitrification. The method comprises the following steps: introducing residual sludge containing a small amount of AOB from an HRBPR section, and periodically adding the residual sludge into a PN / A reactor according to a volume ratio of 0.1-5%; according to the process, segmented DO control is adopted, NOB ecological niche inhibition and short-cut nitrification long-term stability are achieved, and synchronous deep nitrogen and phosphorus removal can be achieved without additional carbon sources and chemical agents. The device has the advantages of low energy consumption, low sludge yield, high operation stability and the like, and is suitable for energy-saving and efficient treatment of urban low-C / N-ratio sewage.

The utility model discloses a short-cut nitrification coupling anaerobic ammonia oxidation integrated reactor, a perforated plate is arranged in the reactor, and a first part for short-cut nitrification and a second part for anaerobic ammonia oxidation are respectively distributed on the upper side and the lower side of the perforated plate; sludge of nitrite bacteria is attached to the interior of the first part through a first filler so as to fix aerobic bacteria, and the first part is connected with an aeration source; anaerobic ammonia oxidizing bacteria are fixed in the second part through a second filler; the size of the filler is greater than the hole size of the perforated plate; the second part is provided with a muddy water backflow and separation module; the muddy water backflow and separation module comprises the muddy water separation area and a sludge backflow area which is integrally connected with the muddy water separation area; a water outlet pipe is connected into the mud-water separation area and is used for separating and discharging aged sludge. The reactor can stabilize the growth of dominant bacteria and improve the denitrification treatment effect.

The invention relates to the technical field of environmental biology, and discloses a short-cut nitrification-denitrification complex microbial inoculant and a preparation method thereof. The method includes; the method comprises the following steps: respectively culturing ammonia oxidizing bacteria, denitrifying bacteria and heterotrophic nitrification-denitrifying bacteria, mixing, and co-domesticating under a low dissolved oxygen condition to obtain a co-domesticated flora; and then adding into sodium alginate-polyvinyl alcohol, immobilizing by a calcium chloride-boric acid-glutaraldehyde system, and freeze-drying to obtain the complex microbial inoculant. The prepared complex microbial inoculant can stably realize synchronous short-cut nitrification and denitrification under the condition of low dissolved oxygen, is high in nitrite accumulation rate, remarkably improves the removal rate of ammonia nitrogen and total nitrogen, has the advantages of stable flora structure, high denitrification efficiency and low operation energy consumption, and is suitable for industrial production. The method can be widely applied to biological nitrogen treatment of urban sewage and high-ammonia-nitrogen industrial wastewater.

The invention discloses ammonia oxidizing bacteria and application thereof, relates to the technical field of microorganisms, in particular to ammonia oxidizing alcaligenes and a symbiotic combination of the ammonia oxidizing alcaligenes and Pseudomonas oleovorans, and the ammonia oxidizing alcaligenes and the Pseudomonas oleovorans have the advantages of being high in biofilm culturing efficiency, short in starting time and high in nitrification efficiency.

The application provides a wastewater treatment method based on interspecies electron transfer, and belongs to the technical field of wastewater treatment. The wastewater treatment method based on interspecies electron transfer comprises the following steps: introducing wastewater to be treated into a wastewater treatment device, wherein the wastewater treatment device comprises ammonia-oxidizing bacteria, carbon-fixing bacteria and sulfate-reducing bacteria; under anaerobic conditions, in the wastewater treatment device, the ammonia-oxidizing bacteria oxidize ammonia nitrogen in the wastewater to be treated as an electron donor to generate an oxidation reaction, the carbon-fixing bacteria reduce inorganic carbon in the wastewater to be treated as an electron acceptor to generate a reduction reaction, and the sulfate-reducing bacteria are used to combine the ammonia-oxidizing bacteria and the carbon-fixing bacteria by using interspecies electron transfer capacity of the sulfate-reducing bacteria, so that the wastewater to be treated is subjected to denitrification through metabolic interaction among multiple strains, and treated wastewater is obtained.

Systems and methods for producing ammonium nitrate are disclosed herein. Exemplary systems can comprise a bioreactor positioned to receive a feed including ammonia. The bioreactor can hold a liquid solution including (i) an ammonia oxidizing bacteria (AOB) and (ii) a nitrite oxidizing bacteria (NOB). The AOB can facilitate oxidation of the ammonia to produce a nitrite at a nitritation rate and the NOB can facilitate oxidation of the nitrite at a nitratation rate. Thus, the bioreactor can produce a mixed liquor comprising a nitrate biologically. The system can further comprise a filter positioned to receive the mixed liquor and produce a permeate and a sludge. A sensor can measure the concentration of the liquid solution of the bioreactor, and a controller coupled to the sensor can regulate the mass flow of nitrogen and / or phosphorus of the bioreactor feed based on the measured concentration of the liquid solution.

The invention discloses a deep denitrification device based on whole-course ammoxidation coupling sulfur autotrophic denitrification and a starting method thereof, and belongs to the technical field of biological sewage treatment. The method comprises the following steps: firstly, inoculating sludge containing whole-course ammonia oxidizing bacteria into a reactor; continuously introducing wastewater containing ammonia nitrogen, and gradually increasing the ammonia nitrogen concentration to enrich whole-course ammonia oxidizing bacteria; finally, sulfide is added into the inlet water, the concentration of the sulfide is gradually increased, and the sulfur autotrophic denitrifying bacteria are enriched. Ammonia nitrogen is converted into nitrate nitrogen through the whole-process ammonia oxidizing bacteria, nitrate nitrogen is further converted into nitrogen through the sulfur autotrophic denitrifying bacteria, and deep denitrification is achieved through the synergistic effect of the ammonia oxidizing bacteria and the sulfur autotrophic denitrifying bacteria in the same reactor. Aeration and an external carbon source are not needed in the operation process, and the energy consumption and the operation cost are remarkably reduced.

This invention proposes an enhanced refolding device and operating method for refrigerated anaerobic ammonia oxidation sludge. The device includes a sampling port straight-tube syringe (1), an inlet straight-tube syringe (2), a three-way valve (3), a magnetic stirrer (4), an anode (8), a cathode (9), a changeover switch (10), an air bag (11), a reaction vessel, a power supply assembly, an air inlet (a) and an air outlet (b) of a hose syringe. The power supply assembly includes a voltage data acquisition system (5), a DC regulated power supply (6), and a resistor (7). In this invention, an additional volume of activated sludge is added as an enhanced refolding factor during the mainstream wastewater denitrification process. This accelerates the recovery of the activity of refrigerated anaerobic ammonia oxidizing bacteria, improves the overall denitrification efficiency of the system, and promotes the operation of the mainstream anaerobic ammonia oxidation system under two different electron acceptor conditions: nitrite and electrodes.

This invention relates to a sulfur autotrophic denitrification coupled with anaerobic ammonia oxidation filter and its operation method based on a backwashing strategy to achieve hydrogen sulfide reduction and sulfur source regeneration, belonging to the field of biological wastewater treatment. A backwashing device is installed at the bottom of the filter, and sulfur-containing filter media is dispersed within the filter. The filter outlet is equipped with a dissolved oxygen coupled with a temperature probe and a hydrogen sulfide probe, connected to a real-time monitoring and control system. The sulfur-containing filter media serves as a carrier for the attachment and growth of sulfur autotrophic denitrifying bacteria, sulfate-reducing bacteria, and anaerobic ammonia oxidizing bacteria. The real-time monitoring and control system is configured to activate the backwashing device when the hydrogen sulfide concentration exceeds a set value, injecting a set amount of gas into the filter for backwashing. While ensuring the smooth progress of sulfur autotrophic denitrification, anaerobic ammonia oxidation is coupled, and the oxygen from the backwashing is used to oxidize the hydrogen sulfide, converting it into elemental sulfur, which continues to serve as a sulfur source to drive sulfur autotrophic denitrification and achieve sulfur source regeneration. This effectively reduces the hydrogen sulfide content.

The application discloses a use of carvacrol as a nitrification inhibitor, a use method of carvacrol as the nitrification inhibitor, and a fertilizer manufacturing method and a fertilizer formed by the method. The application finds through indoor simulation, field plot and field tests that the carvacrol has a good inhibiting effect on soil ammonia-oxidizing bacteria when the use amount of the carvacrol is 5.0-15.0 g per mu, can effectively delay the conversion of ammonium nitrogen into nitrate nitrogen, and has an advantage over a traditional nitrification inhibitor in inhibiting a late nitrification stage.

A device and method for rapidly improving the deep denitrification of high ammonia nitrogen wastewater based on a PNA continuous flow IFAS process belongs to the field of biological denitrification of high ammonia nitrogen wastewater with low C / N ratio. The device includes a raw water tank, an IFAS reactor, an MBR (Medium-Range Bioreactor), and a sedimentation tank; the raw water tank and IFAS reactor are sequentially connected to the sedimentation tank and MBR at different stages. The method includes the following steps: In the stable operation stage, the IFAS reactor is fed with water, and low-oxygen aeration is performed, with effluent from the sedimentation tank. In the fluctuating operation stage, the IFAS reactor is continuously fed with water, and the sedimentation tank is replaced with the MBR, with the effluent from the sedimentation tank becoming membrane effluent. In the new steady-state stage, the IFAS reactor is continuously fed with water, and the MBR is replaced back with the sedimentation tank, with the effluent from the membrane reverting to gravity sedimentation. This invention is applicable to the deep denitrification process of high ammonia nitrogen wastewater generated from landfill leachate based on short-cut nitrification coupled with anaerobic ammonia oxidation, effectively enriching anaerobic ammonia oxidizing bacteria. The device is simple and has significant energy-saving and consumption-reducing advantages.

To provide a water treatment method and a water treatment apparatus capable of more appropriately and stably maintaining the generation of nitrite nitrogen in a nitritation process and more efficiently performing nitrification and denitrification treatment.SOLUTION: A water treatment method for biologically treating raw water containing a nitrite component includes a nitrite-forming step of oxidizing ammoniacal nitrogen in the raw water to nitrite-nitrogen with biosludge containing ammonia-oxidizing bacteria, a nitrite treatment step of extracting a part of the biosludge in the nitrite-forming step and bringing the extracted biosludge into contact with a nitrite aqueous solution at a pH6 of less than 0 and a nitrite-nitrogen concentration of less than 250mg - N / L for 48 hours or less to suppress activity and proliferation of the nitrite-oxidizing bacteria contained in the biosludge, and a returning step of returning the biosludge after the nitrite treatment step to the nitrite-forming step.SELECTED DRAWING: Figure 1

The invention relates to the technical field of foul smell treatment in livestock and poultry breeding, in particular to a compound livestock and poultry manure deodorant as well as a preparation method and application thereof. The deodorant comprises a compound microorganism culture solution, a plant compound extracting solution and a chemical absorbent, the compound microorganism culture solution comprises a culture solution of sulfur oxidizing bacteria, a culture solution of dimethyl sulfide degrading bacteria, a culture solution of trimethylamine degrading bacteria and a culture solution of ammonia oxidizing bacteria; the plant composite extracting solution comprises a plant extracting solution and a surfactant; the raw materials of the plant extracting solution are selected from one or more of honeysuckle, raspberry and clove; the chemical absorbent comprises zinc diricinoleate. The composite livestock and poultry manure deodorant provided by the invention is simple in process and relatively low in cost, has the characteristics of fast deodorization effect and lasting effect, and is suitable for popularization and application in production practice.

The application discloses a continuous flow AOA treatment device based on intermittent light strategy for rapid start-up of short-cut nitrification in the field of sewage biological technology, which comprises a raw sewage tank, an AOA reactor, a sedimentation tank and a side stream reactor. Sewage from the raw sewage tank enters the AOA reactor, and sequentially passes through an anaerobic zone, an aerobic zone and an anoxic zone along the water flow direction, and part of the sewage is shunted from the anaerobic zone to the side stream reactor; the anaerobic zone mainly carries out anaerobic phosphorus release and internal carbon source storage process, the aerobic zone carries out nitrification and aerobic phosphorus absorption, and the anoxic zone carries out endogenous denitrification; by adopting intermittent light in the side stream reactor, nitrite oxidizing bacteria are inhibited, ammonia oxidizing bacteria are enriched, the rapid start-up and long-term stability of short-cut nitrification of the AOA reactor are promoted, and the phosphorus removal efficiency of the process is ensured; the nitrite accumulation rate and the total nitrogen removal rate of the application are both stabilized at more than 80%, and the phosphorus removal efficiency can reach more than 95%, which provides a new technical approach for efficient sewage biological denitrification and phosphorus removal.

This invention relates to an anaerobic ammonia oxidizing microbial denitrification system, comprising a tank, a separator connected to the tank, and multiple aeration heads disposed at the bottom of the tank. The system is characterized by a first protective mechanism disposed on the aeration heads to prevent anaerobic ammonia oxidizing bacteria sludge particles from adhering to and multiplying on the aeration heads, thus preventing blockage. By incorporating this first protective mechanism, the invention, through the combined action of a sealing component and an opening / closing component, ensures that the aeration heads remain in a closed environment when not in operation. Simultaneously, in the closed state, a cleaning component promptly disperses and discharges the sludge from the top of the aeration heads. This solves the technical problem that anaerobic ammonia oxidizing bacteria easily enter the micropores of the aeration heads during their reproduction process, leading to blockage, reduced aeration effect, slower reaction between anaerobic ammonia oxidizing bacteria sludge particles and wastewater, and decreased denitrification efficiency.

This disclosure provides, inter alia, systems and methods for testing products for ammonia oxidizing bacteria, quality control, and providing products with ammonia oxidizing bacteria. The systems and methods herein may be used, inter alia, to treat diseases associated with low nitrite levels, skin diseases, and diseases caused by pathogenic bacteria.

This invention discloses an apparatus and method for achieving deep nitrogen removal and phosphorus recovery through dual short-range coupled anammox fermentation using in-situ sludge fermentation. First, calcium chloride solution is added to the anammox reactor at the rear end of a two-stage PNA process to form recoverable phosphorus crystals such as Ca-P precipitates. Anaerobic ammonia oxidizing bacteria attach to the surface of the precipitates, thereby enriching and retaining them within the anammox reactor. During the continuous formation of anaerobic ammonia oxidizing particles, unfermented waste activated sludge is added to the PNA process. The slow hydrolysis and acidification of the waste activated sludge provide a carbon source for the short-range denitrifying bacteria, completing the transformation from single-stage PNA autotrophic nitrogen removal to dual short-range coupled anammox synergistic nitrogen removal. In this invention, the anaerobic ammonia oxidizing bacteria in the granular sludge after operation can reach 12%, 73% of the sludge exhibits a particle size greater than 200 μm, and the total nitrogen removal rate remains above 90%, with most phosphorus existing in the recoverable form of Ca-P crystals.

The invention belongs to the field of environmental protection water treatment and resource regeneration, and discloses a two-stage biological denitrification sewage treatment method and system. According to the method, sewage is subjected to short-cut nitrification / anaerobic ammonia oxidation primary denitrification in a first-stage device through HAP sludge particles containing ammonia oxidizing bacteria (AOB) and anaerobic ammonia oxidizing bacteria (AnAOB), and then is subjected to short-cut denitrification and anaerobic ammonia oxidation deep denitrification in a second-stage device through a biological membrane filler attached with short-cut denitrifying bacteria (PDB) and anaerobic ammonia oxidizing bacteria (AnAOB); caCl2 can be added into the HAP sludge particles according to the P concentration of inlet water to generate HAP crystals, so that settling solid-liquid separation is realized for recycling. According to the invention, multi-path synchronous removal of ammonia nitrogen (NH4 +), nitrite (NO2-) and nitrate (NO3-) is realized synchronously, and the energy consumption is reduced by more than 60% compared with that of a traditional denitrification process; an external carbon source is reduced by more than 50%, the sludge yield is reduced by 50-60% compared with that of a traditional denitrification system, and the sludge treatment cost is reduced.

The invention provides an aeration power transverse internal circulation ultra-short HRT biological denitrification device and process. According to the denitrification device, the reaction area is divided into the descending area and the ascending area by the partition plate, and a relatively high internal circulation flow rate is formed by driving by utilizing residual kinetic energy generated by aeration and a density difference between the two areas; nO2 <-> generated by ammonia oxidizing bacteria in an aerobic environment is rapidly transferred to an anoxic environment through a flow field to be reduced by anaerobic ammonia oxidizing bacteria and denitrifying bacteria, and meanwhile, proliferation of NOB is limited. Besides, eddy current formed near the top and bottom gaps of the partition plate has strong hydraulic shear force, formation and stability of granular sludge can be promoted under low HRT (within 2h), and stable and efficient biological denitrification performance is realized. According to the invention, a particle-floc two-phase biological denitrification process is adopted, and floc sludge serves as a protective layer of granular sludge, so that the influence of external factors such as dissolved oxygen, organic matters and the like is relieved. The process has relatively high biological load and relatively strong applicability, and can adapt to sewage with different water quality characteristics.

This invention provides a short-cut denitrification coupled with anaerobic ammonia oxidation composite biofilm based on carbon load regulation, its preparation method, and its application, belonging to the field of wastewater treatment technology. The method of this invention includes the following steps: adding biofilm packing material enriched with denitrifying bacteria inside a sequencing batch reactor (SBR); adjusting the influent flow rate and carbon source dosage; gradually reducing the influent carbon-to-nitrogen ratio (C / N ratio); and in-situ enriching anaerobic ammonia oxidizing bacteria on the biofilm packing material to form a composite biofilm with symbiotic short-cut denitrifying bacteria and anaerobic ammonia oxidizing bacteria. The gradual reduction of the influent C / N ratio includes multiple continuous and stable operation stages. This invention directionally enriches anaerobic ammonia oxidizing bacteria on existing denitrifying bacterial membranes, resulting in a strong synergistic effect of functional bacterial communities. Under a C / N ratio of 2.5, the anaerobic ammonia oxidation nitrogen removal contribution rate is ≥60%, and the total nitrogen removal rate is consistently above 70%. It has advantages such as no need for additional inoculation, short construction cycle, and stable nitrogen removal efficiency.

This invention relates to the field of wastewater denitrification technology, and particularly to a method based on S n 2‑ A method and system for one-stage PD / A deep denitrification using a mediated effect includes: constructing a biofilm reaction system by setting up a biofilm carrier component and inoculating it with activated sludge in a continuous flow reactor; simulating the preparation of wastewater effluent containing nitrate nitrogen and introducing a polysulfide solution as an electron donor, then independently and continuously adding both to the continuous flow reactor and mixing them uniformly by stirring; adding ammonia nitrogen to the wastewater effluent and inducing in-situ enrichment of anaerobic ammonia oxidizing bacteria in the biofilm reaction system by staged control of the ammonia nitrogen load in the wastewater effluent; and achieving stable coupling of short-cut denitrification and anaerobic ammonia oxidation in a one-stage continuous flow reactor to simultaneously remove nitrate nitrogen and ammonia nitrogen from the wastewater effluent. This invention features a simple start-up method, stable operation, low dependence on external organic carbon sources, and low sludge yield, making it suitable for deep denitrification treatment of urban wastewater with low carbon-to-nitrogen ratios and wastewater treatment plant effluent.