123 results about "Ammonia-oxidizing bacteria" patented technology

The invention discloses a high-activity short-cut nitrification quick-start long-term stable operation process, which relates to the technical field of biological sewage treatment, and comprises the following steps: inoculating sludge, carrying out anaerobic fermentation pretreatment, and removing expanded sludge and sludge foam in combination with quick sludge discharge and manual removal; controlling the number of days of sludge age, reproliferating the sludge, enriching ammonia oxidizing bacteria, and inhibiting and elutriating nitrite oxidizing bacteria; long-term operation is carried out under the condition of no sludge discharge, the abundance and activity of ammonia oxidizing bacteria are maintained, and meanwhile, the abundance of nitrite oxidizing bacteria is controlled; the proliferation of nitrite oxidizing bacteria is controlled by using the predation effect of protozoa and metazoan, and the accumulation rate of nitrite in the reactor is controlled; monitoring the dissolved oxygen, the pH value and the concentrations of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the reactor in real time, and regulating and controlling the influent ammonia nitrogen load and the free ammonia concentration; dissolved oxygen distribution is adjusted in an intermittent aeration or microporous aeration mode, and the operation conditions of the short-cut nitrification reactor are optimized. The method is simple to operate, economical and effective.

The invention discloses a device and a method for realizing low-carbon-nitrogen-ratio sewage autotrophic and heterotrophic coupled deep nitrogen and phosphorus removal based on a continuous flow membrane aeration bio-membrane reactor. A hollow fiber membrane is arranged in the continuous flow reactor, and an oxygen concentration gradient is formed through a bubble-free membrane aeration technology. In the biomembrane area, spatial partitions of functional bacteria such as nitrifying bacteria and anaerobic ammonium oxidation bacteria are established, and the two anoxic areas realize efficient nitrogen removal through short-cut denitrification coupling anaerobic ammonium oxidation (PD / A) and synchronous nitrification and denitrification. In the aspect of phosphorus removal, the system adopts an anaerobic-aerobic operation mode, phosphorus-accumulating bacteria release phosphorus anaerobically and absorb excessive phosphorus aerobically, and finally, phosphorus removal is realized through residual sludge discharge. The system integrates an internal carbon source extraction process, recovers a sludge carbon source and supplements an electron donor required by denitrification, and reduces the operation cost and carbon emission. The continuous flow MABR denitrification system enhances the denitrification efficiency of the PD / A system, is suitable for large-scale operation, and efficiently and stably realizes deep denitrification of a low-carbon-nitrogen-ratio urban sewage treatment plant.

The invention provides a high-hydrophilic nano-iron-rich polyurethane bio-enriched filler as well as a preparation method and application thereof, and belongs to the technical field of polyurethane foam fillers. The high-hydrophilic nano-iron-rich polyurethane bio-enriched filler provided by the invention is prepared from the following components in parts by mass: 50 to 70 parts of liquefied starch alcohol, a component A, a component B and 0.2 to 0.3 part of nano-iron powder, wherein the component A is prepared from 130 to 150 parts of polyether polyol, 8 to 10 parts of a foaming agent, 0.7 to 0.9 part of a foam stabilizer and 0.1 to 0.3 part of a gel catalyst; and the component B comprises the following components in parts by weight: 123-125 parts of isocyanate and 0.1-0.3 part of a catalyst. The high-hydrophilic nano-iron-rich polyurethane bio-enrichment filler provided by the invention has good hydrophilicity and specificity, can better adsorb and enrich anaerobic ammonium oxidation bacteria, can bear larger load impact, is not easy to block, and improves the treatment efficiency while ensuring the stable operation of a treatment system.

The invention provides application of 3-methyl-2-oxazolidinone as a nitrification inhibitor and a nitrification inhibition method, and relates to the technical field of soil treatment. Specifically, the invention relates to application of 3-methyl-2-oxazolidinone as a nitrification inhibitor in soil treatment and / or sewage treatment and inhibition of nitrosation activity of ammonia oxidizing bacteria. The method for inhibiting nitrification comprises the step of treating soil or water containing ammonium nitrogen and ammonia oxidizing bacteria by using 3-methyl-2-oxazolidinone. The concentration of the 3-methyl-2-oxazolidinone is not less than 0.01% w / v by taking the volume of the soil or the water body as 100%. The nitrification inhibition activity of the 3-methyl-2-oxazolidinone is found, so that the variety selection of nitrification inhibitors is enriched.

Systems and methods for producing ammonium nitrate are disclosed herein. Exemplary systems can comprise a bioreactor positioned to receive a feed including ammonia. The bioreactor can hold a liquid solution including (i) an ammonia oxidizing bacteria (AOB) and (ii) a nitrite oxidizing bacteria (NOB). The AOB can facilitate oxidation of the ammonia to produce a nitrite at a nitritation rate and the NOB can facilitate oxidation of the nitrite at a nitratation rate. Thus, the bioreactor can produce a mixed liquor comprising a nitrate biologically. The system can further comprise a filter positioned to receive the mixed liquor and produce a permeate and a sludge. A sensor can measure the concentration of the liquid solution of the bioreactor, and a controller coupled to the sensor can regulate the mass flow of nitrogen and / or phosphorus of the bioreactor feed based on the measured concentration of the liquid solution.

A mainstream anaerobic ammonia oxidation enhanced denitrification device and method based on a fluidized bed biofilm. By arranging reaction tanks, switching of two operation modes can be achieved, and recovery of instability in starting and running periods is completed. A denitrification pathway comprises: implementing denitrification and partial denitrification-anaerobic ammonia oxidation synergy in an anoxic zone; and implementing nitrification, synchronous nitrification and denitrification, and partial nitrification-anaerobic ammonia oxidation synergy in an aerobic zone. Implementation of anaerobic ammonia oxidation for denitrification in the anoxic zone or the aerobic zone requires first enhancement of an aerobic biofilm, enhancement of the thickness of the biofilm under the action of high DO, and formation of an internal anoxic / anaerobic layer, thereby providing conditions for enrichment of anaerobic ammonia oxidizing bacteria.

The invention belongs to the field of industrial wastewater treatment, and relates to an iron autotrophic denitrification coupling anaerobic ammonia oxidation combined enrichment culture device and method. Comprising a water inlet tank, a No.1 anoxic reactor, an intermediate tank and a No.2 anaerobic reactor, the water inlet tank is provided with a water outlet pipeline of the water inlet tank, and the water outlet pipeline of the water inlet tank is connected with the No.1 anoxic reactor; the anoxic reactor I is provided with a water outlet pipeline of the anoxic reactor I, and the water outlet pipeline of the anoxic reactor I is connected with the intermediate tank; the intermediate tank is provided with an intermediate tank water outlet pipeline, and the intermediate tank water outlet pipeline is connected with the No.2 anaerobic reactor; the No.2 anaerobic reactor is provided with a No.2 anaerobic reactor water outlet pipeline; the No.2 anaerobic reactor water outlet pipeline is connected with the No.1 anoxic reactor. According to the invention, collaborative culture of autotrophic denitrification bacteria and anaerobic ammonium oxidation bacteria can be realized, an autotrophic denitrification effect provides a certain amount of nitrite and iron ions to promote anaerobic ammonium oxidation reaction, and an anaerobic ammonium oxidation effect can effectively prevent an autotrophic denitrification tank body from being mineralized.

The present invention belongs to the technical field of sewage treatment. Disclosed are a biological carrier for enhancing enrichment and activity of anaerobic ammonium oxidation bacteria, a preparation method therefor, and a use thereof. In the present invention, a functional biological carrier suitable for anaerobic ammonium oxidation is prepared by blending polyethylene or polypropylene as a basic raw material, a positively charged polymer such as a polyquaternary ammonium salt or a cationic polyacrylamide as a modifying material, and zero-valent iron and a carbon-based material containing C=O and -COOH functional groups as functional materials. The biological carrier of the present invention can enhance the enrichment and metabolic activity of the anaerobic ammonium oxidation bacteria, thereby improving the nitrogen removal efficiency.

The invention relates to a soil conditioner based on nitrification inhibition and a soil acid control method, and belongs to the technical field of soil conditioning, the soil conditioner comprises the following components by weight: 20-22 parts of humic acid, 32-36 parts of syringic acid-linoleic acid modified porous hydroxyapatite, 4-5 parts of complete nitrifying bacteria, 2-5 parts of syringic acid, and 2.8-4.2 parts of sodium carboxymethyl cellulose. According to the technical scheme, syringic acid and linoleic acid serve as nitrification inhibitors, the nitrification inhibition rate can be effectively increased, ecological niches of ammonia-oxidizing archaea and ammonia-oxidizing bacteria can be reduced through competitive occupation due to addition of complete nitrifying bacteria, the nitrification rate of the ammonia-oxidizing archaea and the ammonia-oxidizing bacteria is inhibited, and N2O emission is reduced. Humic acid and hydroxyapatite can be used as pH buffer substances to regulate and control soil acidity, aluminum toxicity can be relieved, and physical and chemical properties of soil can be improved; meanwhile, the complete nitrifying bacteria can be ensured to have relatively high activity under the condition that the pH is 5.5-6.5.

The invention relates to the technical field of wastewater treatment or water quality purification, in particular to a method for synchronously culturing ammonia oxidizing bacteria and nitrite oxidizing bacteria, which comprises the following steps: selecting an inorganic material which is large in specific surface area and can quickly neutralize and release alkalinity as a nitrifying bacteria attachment carrier and an alkalinity supplement / pH regulator; the carrier is dissolved through nitration acid production, carbon dioxide and alkalinity are released, a carbon source and alkalinity required by growth are provided for nitrifying bacteria, and propagation of the nitrifying bacteria is promoted. Ammonia oxidizing bacteria and nitrite oxidizing bacteria are cultured synchronously by controlling the growth speed of the two bacteria in the culture process. By controlling the ammonia nitrogen concentration and nitrite concentration of the effluent at the end of culture to be lower than 50mg / L, sufficient time is provided for nitrite oxidizing bacteria to decompose an intermediate product nitrite, so that the purpose of simultaneously culturing AOB and NOB is achieved.

The present invention discloses a novel device for culturing and proliferating anaerobic ammonia-oxidizing bacteria, including a reactor, a water inlet mechanism, a negative pressure forming mechanism, a low-voltage power supply, an anode, a cathode, a constant temperature mechanism and a water outlet mechanism. The reactor is provided with a reaction tank body and a water bath from the inside to the outside, the water bath is wrapped around the outside of the reaction tank body, a containing chamber is provided in the reaction tank body, the water inlet mechanism is connected to the bottom of the containing chamber, the water outlet mechanism is connected to the upper part of the containing chamber, the negative pressure forming mechanism is installed above the reactor and is connected to the containing chamber, and is used to form a negative pressure environment in the containing chamber, the anode and the cathode are symmetrically installed in the containing chamber, and are respectively electrically connected to the positive and negative poles of the low-voltage power supply, and the constant temperature mechanism is connected to the water bath, and the water in the water bath is circulated and kept warm. The present invention adopts a novel device that combines a specific negative pressure with a low voltage and a specific reaction tank body, and cooperates with a culturing method to achieve rapid recovery of the activity of anaerobic ammonia-oxidizing sludge and promote the proliferation of anaerobic ammonia-oxidizing bacteria.

The invention discloses a method for realizing deep autotrophic nitrogen removal of sludge digestion liquid based on alternate intermittent aeration of a double-membrane aeration bio-membrane reactor (MABR). The process device comprises a sludge digestion liquid raw water tank, a single-stage double-MABR reactor, two MABR devices and a water outlet tank. According to the method, sludge digestion liquid enters the single-stage double-MABR reactor, the two MABR devices are alternately and intermittently aerated, the state of one aeration and one aeration stopping is always kept, and ammonia nitrogen in raw water is converted into nitrite through ammonia oxidizing bacteria enriched on the inner side of an MABR membrane. And simultaneously supplying to anaerobic ammonium oxidation bacteria enriched on the outer sides of the two MABR membranes through matrix diffusion for denitrification and generation of nitrate. Denitrifying bacteria enriched on the outer side of the MABR membrane utilize raw water organic matters to drive short-cut denitrification to synchronously supply nitrite to anaerobic ammonium oxidation bacteria so as to realize deep autotrophic nitrogen removal. According to the invention, a double-anaerobic ammonia oxidation reaction hot area is constructed through alternate intermittent aeration of double MABRs, and efficient deep autotrophic nitrogen removal of the sludge digestion liquid can be stably realized.

The invention discloses a method for in-situ enrichment of anaerobic ammonia oxidizing bacteria, a bio-membrane reactor is adopted, and a carrier of a bio-membrane is made of a material with good conductivity and biological affinity; the direct-current power supply continuously outputs weak voltage below 1.0 V; preparing special wastewater as inlet water of the reactor; sludge containing anaerobic ammonium oxidation bacteria is selected as inoculation sludge, the hydraulic retention time of the reactor is controlled, microorganisms in the reactor mainly grow on a carrier in an attached mode to form a biological membrane, and suspended sludge is eliminated as much as possible; aeration is performed on the reactor, and water enters and exits the reactor continuously. According to the method, the anaerobic ammonium oxidation bacteria are enriched under the micro-aerobic condition, the nitrogen source is only ammonia nitrogen, nitrite nitrogen is generated in the ammonia nitrogen conversion process instead of being supplied externally, the normal form of anaerobic ammonium oxidation enrichment culture is innovated through the method, and the anaerobic ammonium oxidation enrichment culture efficiency is improved. The problem that anaerobic ammonia oxidizing bacteria cannot be enriched due to lack of external supply of nitrite nitrogen in the actual wastewater treatment process is effectively solved.

This invention relates to an optimized control method for the enrichment of anaerobic ammonia-oxidizing bacteria based on niche regulation, comprising: S1, selecting key niche indicators of constructed wetlands as investigation factors, differentially setting the investigation factors to form experimental systems with different niches, conducting constructed wetland control and monitoring, and collecting data; S2, using the relative abundance and / or absolute abundance of anaerobic ammonia-oxidizing bacteria collected in S1 as dependent variables and the key niche indicators as independent variables, fitting and then predicting a model; S3, making predictions using the prediction model, comparing the prediction results with the control target, and optimizing the control of anaerobic ammonia-oxidizing bacteria enrichment in constructed wetlands based on the comparison results. This method can obtain a prediction model with high prediction accuracy, and then, by adjusting the niche indicators in constructed wetlands, the enrichment of anaerobic ammonia-oxidizing bacteria can be ensured, wastewater treatment efficiency can be improved, and a better total nitrogen removal effect can be achieved.

The invention relates to the technical field of paper cups, and discloses a compound bacterium for degrading nitrite in an aquaculture water body, the compound bacterium is composed of nitrifying bacteria, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist, the nitrifying bacteria are mixed flora of autotrophic ammonia oxidizing bacteria and heterotrophic nitrifying bacteria, and the penetrant is a mixture of bacillus coagulans, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist. The denitrifying bacteria comprise a synergistic effect system of the Pseudomonas (Pseudomonas) and the Paracoccus (Paracoccus), and the Pseudomonas (Pseudomonas) and the Paracoccus (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas)) are The dual-path directional conversion of nitrite is realized through the synergistic effect of constructed nitrification-denitrification synergistic flora and a functional aid (polyethylene glycol-SDS-L-glutamine system). Parameter control shows that in a water body (NO2 <-> is equal to 2.5 mg / L) in the later culture period of the penaeus vannamei boone, the degradation rate reaches 81.4% within 48 hours, and the nitrate increment is controlled within 12%. The technology breaks through the bottleneck of failure of a traditional microbial agent in a high-salinity (35 per mill) and high-density culture environment, and is suitable for sea and fresh water whole-variety aquaculture.

A method for manufacturing a liquid fertilizer product, including the steps of removing solids from organic waste to obtain a filtrate; converting ammonia present in the filtrate to nitrate using ammonia oxidizing bacteria and nitrite oxidizing bacteria to form a converted stream; and removing water from the converted stream to obtain the liquid fertilizer product, wherein the liquid fertilizer product includes nitrate.

This invention discloses a continuous-flow anaerobic bypass IFAS-AOA device and method for treating inert high-ammonia nitrogen wastewater, belonging to the field of biological treatment technology for high-ammonia nitrogen wastewater. The device consists of three parts: a raw water storage tank, a continuous-flow anaerobic bypass AOA reactor, and a sludge-water separation system. The effluent from the end of the anaerobic process enters the aerobic zone of the AOA reactor, while the anaerobic end-mixed liquor bypasses to the anoxic zone, mixes with the aerobic end-effluent, flows through the anoxic zone, and enters the sludge-water separation system. In the anaerobic zone, endogenous denitrifying bacteria store organic matter from the raw water as an intracellular carbon source and bypass to the anoxic zone. In the aerobic zone, short-cut nitrification coupled with anaerobic ammonia oxidation serves as the main nitrogen removal pathway. In the anoxic zone, anaerobic ammonia oxidation coupled with endogenous denitrification achieves deep nitrogen removal. The existence of anaerobic bypass allows some of the mixed liquor to bypass the aerobic zone and directly bypass to the anoxic zone, thus fully utilizing the carbon source in the raw water. This invention can achieve stable short-cut nitrification and effective retention of anaerobic ammonia oxidizing bacteria, showing broad application prospects.

The invention provides a sewage treatment method based on interspecific electron transfer, and belongs to the technical field of sewage treatment. The sewage treatment method based on interspecific electron transfer comprises the following steps: introducing sewage to be treated into a sewage treatment device, wherein the sewage treatment device comprises ammonia oxidizing bacteria, carbon sequestration bacteria and sulfate reducing bacteria; under an anaerobic condition, in a sewage treatment device, ammonia oxidizing bacteria take an oxidation reaction by taking ammonia nitrogen in to-be-treated sewage as an electron donor, carbon sequestration bacteria take a reduction reaction by taking inorganic carbon in the to-be-treated sewage as an electron acceptor, and the ammonia oxidizing bacteria and the carbon sequestration bacteria are combined by utilizing interspecific electron transfer capability of sulfate reducing bacteria; the to-be-treated sewage is denitrified through metabolism interaction among multiple strains, and treated sewage is obtained.

The invention relates to the technical field of soil conditioners and applications thereof, and discloses a soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum and an application thereof in ploughing ability improvement, the soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum comprises an organic pollutant degradation functional flora, a soil carbon sequestration functional flora and a small molecular organic matter production functional flora; the organic pollutant degrading functional flora comprises efficient pollutant degrading bacteria; the soil carbon sequestration functional flora comprises ammonia oxidizing bacteria, ammonia oxidizing archaea, complete ammonia oxidizing bacteria, sulfate reducing bacteria, iron reducing bacteria, rhizobium and photosynthetic flora; the micromolecular organic matter production functional flora comprises heterotrophic nitrification-aerobic denitrification bacteria, zoogloea, phosphorus-accumulating bacteria and photosynthetic bacteria. According to the scheme, multiple florae are specifically arranged in the synergistic agent, and through the synergistic interaction effect of the florae, the effects of the synergistic agent in multiple fields of soil carbon sequestration, pollution reduction and yield increase are effectively improved.

The invention discloses a starting and control method for treating ammonia-nitrogen wastewater by an integrated complete ammoxidation coupling short-cut denitrification anaerobic ammoxidation process, and belongs to the field of wastewater biological treatment. The starting method adopts a step-by-step inoculation strategy, and comprises the following steps: firstly, inoculating complete ammoxidation sludge in the SBR reactor for low-oxygen domestication, and after successful starting, supplementing and inoculating short-cut denitrification coupled anaerobic ammoxidation sludge. The control method adopts a sectional water inlet mode, and comprises the following steps: a first part of ammonia nitrogen wastewater firstly enters an integrated SBR (Sequencing Batch Reactor), and ammonia nitrogen is directly oxidized into nitrate nitrogen through complete ammonia oxidizing bacteria (Comammox) under the condition of low dissolved oxygen; aeration is stopped, a carbon source is added for short-cut denitrification, and nitrate nitrogen is reduced into nitrite nitrogen; and then pumping a second part of ammonia nitrogen wastewater, and converting ammonia nitrogen in the wastewater and accumulated nitrite nitrogen into nitrogen by using anaerobic ammonium oxidation bacteria, so as to remove the ammonia nitrogen in the wastewater. According to the invention, deep denitrification is realized under the condition of saving energy consumption and a carbon source.

The present disclosure provides a sewage treatment method and an execution system, comprising: under the conditions of stirring and anaerobic, absorbing and releasing phosphate by anaerobic phosphorus release and internal carbon source conversion reaction in the first sewage, taking the carbon-containing organic matter in the first sewage as a carbon source, to obtain the second sewage; performing aerobic treatment on the second sewage, determining the relative activity of aerobic phosphorus accumulating bacteria and aerobic ammonia-oxidizing bacteria in the second sewage according to the pH value change trend of the second sewage; treating the second sewage by using a multi-stage aeration mode according to the relative activity, adjusting the dissolved oxygen concentration by adjusting the aeration mode of the multi-stage aeration, to occur integrated nitritation-anammox and aerobic phosphorus absorption reaction, to obtain the third sewage; under the conditions of stirring and anaerobic, performing deep phosphorus and nitrogen removal on the third sewage by anaerobic ammonia oxidation reaction, denitrification phosphorus removal reaction and internal carbon source denitrification reaction to obtain the fourth sewage; standing the fourth sewage to separate the sludge and water, and outputting the sewage obtained after the sludge and water separation.

This invention relates to an optimized control method for total nitrogen removal and anaerobic ammonia-oxidizing bacteria enrichment in constructed wetlands, comprising: S1, selecting control factors for the constructed wetland, forming an experimental system using a central coincidence design method, introducing wastewater to be treated into the constructed wetland, monitoring the operation of the constructed wetland, and collecting data on total nitrogen removal and relative abundance of bacteria; S2, using analysis of variance to determine a suitable model method for data fitting, and then constructing prediction models for total nitrogen removal efficiency and relative abundance of anaerobic ammonia-oxidizing bacteria based on the model method; S3, predicting the total nitrogen removal efficiency and relative abundance of anaerobic ammonia-oxidizing bacteria in the constructed wetland, thereby achieving optimized control of the operation and maintenance management of the constructed wetland. The optimized control method provided by this invention can predict the relative abundance of anaerobic ammonia-oxidizing bacteria and the total nitrogen removal efficiency of constructed wetlands, guiding the operation and maintenance management of constructed wetlands.

The invention provides a two-stage HRBPR-PN / A process and device based on sludge biological enhancement. In the front section of the process, an endogenous carbon source is taken in and phosphate is released in an anaerobic stage through phosphorus-accumulating bacteria (PAOs), and then excessive phosphorus is absorbed in an aerobic stage. And the rear stage is an anaerobic / aerobic alternate operation short-cut nitrification anaerobic ammonia oxidation (PNA) process, under the condition of low dissolved oxygen, ammonia oxidizing bacteria AOB and anaerobic ammonia oxidizing bacteria AnAOB cooperate to efficiently remove nitrogen, and meanwhile, residual organic matters in the previous stage and nitrate in the system are removed by strengthening endogenous denitrification. The method comprises the following steps: introducing residual sludge containing a small amount of AOB from an HRBPR section, and periodically adding the residual sludge into a PN / A reactor according to a volume ratio of 0.1-5%; according to the process, segmented DO control is adopted, NOB ecological niche inhibition and short-cut nitrification long-term stability are achieved, and synchronous deep nitrogen and phosphorus removal can be achieved without additional carbon sources and chemical agents. The device has the advantages of low energy consumption, low sludge yield, high operation stability and the like, and is suitable for energy-saving and efficient treatment of urban low-C / N-ratio sewage.

The invention discloses a marine shipping marinobacter SND-2 strain as well as a culture method and application of the marine shipping marinobacter SND-2 strain. According to the invention, a heterotrophic synchronous nitrification and denitrification functional strain SND-2 capable of efficiently degrading nitrogen concentration in a marine culture water body under an aerobic condition is separated from a prawn culture pond, and the strain is identified as Marinobacter nauticus according to colonial morphology and 16S rDNA gene sequencing analysis and phylogenetic analysis, the marine marinobacter SND-2 strain is preserved in the China Center for Type Culture Collection on October 15, 2024 (the preservation number is CCTCC NO: M 20242217). The marine marinobacter SND-2 strain belongs to heterotrophic ammonia oxidizing bacteria, has a heterotrophic nitrification-aerobic denitrification function, can efficiently degrade ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and total nitrogen concentration in a marine culture water body under an aerobic condition, has no toxic effect on aquatic animals, and can be applied to ecological restoration of a seawater water environment.

The present invention relates to the field of biogenic nitrogen fertilizer synergists, and in particular to an application of an olive pomace polyphenol extract-hydroxytyrosol as a biogenic nitrogen fertilizer synergist. The biogenic nitrogen fertilizer synergist component prepared by the present invention is an olive pomace polyphenol extract-hydroxytyrosol. After the olive pomace is deoiled, it is extracted under acid hydrolysis conditions, and an alkaline solution of sodium hydroxide is used to adjust the pH value to neutral to obtain the corresponding hydroxytyrosol. The abundance of soil nitrifying microorganisms (ammonia oxidizing bacteria and ammonia oxidizing archaea) and the conversion of mineral nitrogen in the soil can be effectively suppressed, the effectiveness of nitrogen in the soil can be improved, nitrogen can be more effectively absorbed and utilized by crops, the olive pomace is effectively recycled, and the problems of huge environmental pollution and waste of resources caused by the olive pomace can be effectively avoided.

The utility model discloses a short-cut nitrification coupling anaerobic ammonia oxidation integrated reactor, a perforated plate is arranged in the reactor, and a first part for short-cut nitrification and a second part for anaerobic ammonia oxidation are respectively distributed on the upper side and the lower side of the perforated plate; sludge of nitrite bacteria is attached to the interior of the first part through a first filler so as to fix aerobic bacteria, and the first part is connected with an aeration source; anaerobic ammonia oxidizing bacteria are fixed in the second part through a second filler; the size of the filler is greater than the hole size of the perforated plate; the second part is provided with a muddy water backflow and separation module; the muddy water backflow and separation module comprises the muddy water separation area and a sludge backflow area which is integrally connected with the muddy water separation area; a water outlet pipe is connected into the mud-water separation area and is used for separating and discharging aged sludge. The reactor can stabilize the growth of dominant bacteria and improve the denitrification treatment effect.

The invention belongs to the technical field of sewage treatment, and particularly relates to integrated intelligent anaerobic denitrification equipment which comprises an equipment main body, an aeration pipe and an inclined plate filler, a water inlet pipe is arranged at the top of one side of the equipment main body, and the equipment main body is connected with an external wastewater supply mechanism through the water inlet pipe; a backflow pipe is arranged at the bottom of the equipment body, a thermometer is arranged at the top of the equipment body, a dissolved oxygen meter is arranged on one side of the thermometer, an aeration pipe is arranged on one side in the equipment body, and an aeration disc is arranged at the bottom of the aeration pipe. By arranging the aeration disc, the aeration pipe, the inclined plate filler, the guide plate, the biological filler, the reflux pipe and the reflux pump, the equipment consists of three groups of areas, namely an aeration area, a settling area and an ammoxidation biological deoxidation area, and the equipment adopts multifunctional subareas to create an environment suitable for efficient growth of ammoxidation bacteria; the mass-propagated ammonia oxidizing bacteria can form biological cenobium, compared with the traditional process, the volume load is greatly improved, the denitrification efficiency is improved through a biological method, and meanwhile, the operation cost is saved.

The invention relates to the technical field of environmental biology, and discloses a short-cut nitrification-denitrification complex microbial inoculant and a preparation method thereof. The method includes; the method comprises the following steps: respectively culturing ammonia oxidizing bacteria, denitrifying bacteria and heterotrophic nitrification-denitrifying bacteria, mixing, and co-domesticating under a low dissolved oxygen condition to obtain a co-domesticated flora; and then adding into sodium alginate-polyvinyl alcohol, immobilizing by a calcium chloride-boric acid-glutaraldehyde system, and freeze-drying to obtain the complex microbial inoculant. The prepared complex microbial inoculant can stably realize synchronous short-cut nitrification and denitrification under the condition of low dissolved oxygen, is high in nitrite accumulation rate, remarkably improves the removal rate of ammonia nitrogen and total nitrogen, has the advantages of stable flora structure, high denitrification efficiency and low operation energy consumption, and is suitable for industrial production. The method can be widely applied to biological nitrogen treatment of urban sewage and high-ammonia-nitrogen industrial wastewater.

The invention discloses ammonia oxidizing bacteria and application thereof, relates to the technical field of microorganisms, in particular to ammonia oxidizing alcaligenes and a symbiotic combination of the ammonia oxidizing alcaligenes and Pseudomonas oleovorans, and the ammonia oxidizing alcaligenes and the Pseudomonas oleovorans have the advantages of being high in biofilm culturing efficiency, short in starting time and high in nitrification efficiency.

A method for deep synergistic denitrification of urban sewage based on an improved denitrification filter, which relates to a method for deep synergistic denitrification of sewage. It aims to solve the technical problems that the existing deep denitrification of urban sewage requires an external carbon source, has high operating costs, produces secondary pollution, and has poor COD and total nitrogen removal effects. This method: 1. Equipped with an improved denitrification filter deep synergistic denitrification treatment system: 2. Startup: inoculate manganese oxidizing bacteria, denitrifying bacteria, hydrolytic acidifying bacteria and anaerobic ammonia oxidizing bacteria in the system; 3. Stable treatment stage. This method does not require an external carbon source and reduces the demand for carbon sources. The method of the present invention achieves removal rates of 72.40%, 90.59% and 93.38% for COD, ammonia nitrogen and total nitrogen in the secondary effluent of urban sewage treatment plants, respectively. It can be used in the field of deep denitrification treatment of urban sewage.