The invention provides a culture medium for screening an enhanced biological phosphorus removal functional bacterium and a method for screening the enhanced biological phosphorus removal functional bacterium, and relates to a culture medium for screening a phosphorus removal functional bacterium and a method for screening the phosphorus removal functional bacterium. The culture medium and the method solve the problems that the prior method for screening the enhanced biological phosphorus removal functional bacterium has poor direction, the obtained enhanced biological phosphorus removal functional bacterium has small amount of strains, and the culture medium is not suitable for growth of the enhanced biological phosphorus removal functional bacterium. The culture medium for screening can be an anaerobic culture medium, an aerobic culture medium, a solid separation culture medium and a liquid separation culture medium. The method comprises the following steps: 1, preparing the culture mediums; 2, collecting mud; 3, culturing the anaerobic culture medium; 4, culturing the aerobic culture medium; 6, culturing the anaerobic culture medium; 7, circulating step 4 to step 6 for 2 to 4 times; 8, diluting in constant gradient; 9, culturing the solid separation culture medium; 10, culturing the liquid separation culture medium; 11, repeating step 8 to step 10 for 3 to 8 times; and 12, detecting. The method has strong direction, the functional bacterium obtained by screening has large amount, and the culture medium is suitable for growth of the functional bacterium.