16 results about "PHA biosynthesis" patented technology

The present invention addresses the problem of providing a method for producing PHA, the method being capable of suppressing the generation of fine powder. The problem is solved by a method for producing PHA, the method comprising: (a) a step for enzymatically treating a culture solution containing PHA-containing bacterial cells to obtain an aqueous PHA suspension; (b) a step for heating the aqueous suspension obtained in step (a) at a heating rate of 50 °C / sec to 180 °C / sec to a temperature ranging from (Tm-32) °C to (Tm-2) °C (where, Tm is the melting point of the PHA); and (c) a step for cooling the obtained aqueous suspension, wherein the pH of the aqueous suspension during heating is 2.0-5.0.

The present invention relates to a self-cleaving engineered bacterium comprising a metabolic pathway for producing polyhydroxyalkanoate (PHA); the perforin gene and the endolysin gene are expressed under the control of a promoter. According to the method, on the basis of not influencing the dry cell weight and PHA yield of the engineering strain, the self-splitting of the engineering strain after PHA accumulation is completed is realized, the downstream extraction step of PHA biosynthesis is greatly simplified, and the extraction time is shortened.

The technology described herein is directed to engineered chemoautotrophic bacteria and methods of producing sustainable biomolecules. In several aspects, described herein are engineered bacteria and corresponding methods, compositions, and systems for the production of products such as polyhydroxyalkanoates (PHA), sugar feedstocks, and lipochitooligosaccharide (LCO) fertilizers.

The present invention relates to a method for treatment of polyhydroxyalkanoate (PHA) containing post-consumer product, the method comprising contacting a post-consumer product with a polypeptide that can catalyze degradation of the PHA, the contact taking place at a temperature at least 45° C. In a specific embodiment, the poly peptide is a wild-type PHA depolymerase expressed by a thermophilic microorganism or a modified PHA depolymerase that includes one or more single-site mutations as compared to the wild-type PHA depolymerase. In another specific embodiment, the polypeptide comprising a modified poly hydroxy butyrate (PHB) depolymerase comprising one or more single-site mutations as compared to SEQ ID NO: 1, and the modified PHB depolymerase having an optimum temperature of at least 45° C. The present invention also relates to a host cell transformed to express a polypeptide that catalyzes degradation of a PHA, the polypeptide having an optimum temperature for the degradation reaction of at least 45° C., wherein the host cell is selected from an E. coli cell or a thermophilic microorganism.

The present invention relates to a method for treatment of poly hydroxy alkanoate (PHA) containing post-consumer product, the method comprising contacting a post-consumer product with a polypeptide that can catalyze degradation of the PHA, the contact taking place at a temperature at least 40° C. and in the presence of salt at a concentration of 1 M or greater. In a specific embodiment, the polypeptide is a wild-type PHA depolymerase expressed by a halophilic microorganism or a modified PHA depolymerase that includes one or more single-site mutations as compared to the wild-type PHA depolymerase. In another specific embodiment, the polypeptide comprising a modified poly hydroxy butyrate (PHB) depolymerase comprising one or more single-site mutations as compared to SEQ ID NO: 1, and the modified PHB depolymerase having a solubility of 10 mg / L or greater. The present invention also relates to a host cell transformed to express a polypeptide that catalyzes degradation of a PHA in the presence of salt at a concentration of 1 M or greater, wherein the host cell is selected from an E. coli cell or a halophilic microorganism.

A transformed microorganism having the ability to produce a copolymerized polyhydroxyalkanoate containing a 3-hydroxyalkanoate monomer unit having 8 or more carbon atoms, the transformed microorganism having: an exogenous gene encoding a polyhydroxyalkanoate synthase containing an amino acid sequence represented by any one of SEQ ID NO: 1-4; or a foreign gene encoding a protein that includes an amino acid sequence having 90% or more sequence identity with the amino acid sequence represented by any one of SEQ ID NO: 1 to SEQ ID NO: 4, and that has polyhydroxyalkanoate synthase activity.

A carbon-sequestering algal bioplastic, comprising polyhydroxyalkanoate (PHA), sodium alginate, and biochar.

The present invention provides a method for producing PHA that offers excellent separation of PHA from bacterial residue (e.g., residual protein). [Solution] A method for producing PHA, comprising: (a) adding an enzyme to a culture medium containing inactivated bacterial cells that contain PHA, thereby enzymatically treating the bacterial cells; and (b) adding an oxidizing agent having a standard oxidation-reduction potential of 2.00V or higher at 25°C under alkaline conditions to the enzymatically treated solution obtained in step (a), and reacting it under conditions of pH 9.0 to 12.0.

Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and / or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production.

A method for depolymerizing a poly(hydroxyalkanoate) by reaction with a depolymerase enzyme to produce a mixture of poly(hydroxyalkanoate) degradation products. The present disclosure also provides methods for recycling or repurposing the poly(hydroxyalkanoate) to provide a new poly(hydroxyalkanoate) or to produce a biogas feedstock.

A method for depolymerizing a poly(hydroxyalkanoate) by reaction with a depolymerase enzyme to produce a mixture of poly(hydroxyalkanoate) degradation products. The present disclosure also provides methods for recycling or repurposing the poly(hydroxyalkanoate) to provide a new poly(hydroxyalkanoate) or to produce a biogas feedstock.

The problem addressed is to provide a method for producing PHA that can sufficiently remove impurities while reducing the amount of wastewater in a purification process and can realize a practical filtration rate. The problem is solved by a method for producing PHA, the method comprising: a centrifugal separation step in which a PHA aqueous suspension (1) is centrifuged to obtain a PHA aqueous suspension (2) having a predetermined protein content; and a filtration step in which the PHA aqueous suspension (2), which has been adjusted to a pH of from more than 5.5 to 11.0, is subjected to dead end filtration.

The present invention relates to a method for producing polyhydroxyalkanoates (PHAs) in a bacterial strain containing the phaCAB operon and in the presence of low-protein, low-lactose dairy wastewater. The method of the present invention can be used in a medium- to large-scale industrial process for the production of PHAs, preferably PHB.

The present application relates to the field of microbiological technology, and particularly relates to a viscous polyhydroxyalkanoate, and its preparation and use. The viscous polyhydroxyalkanoate can be produced by fermenting a Pseudomonas strain containing a mutant of PHA polymerase PhaC61-3, and can be used for the preparation of biodegradable products.

The present invention addresses the problem of providing a method for producing PHA, the method being capable of suppressing a decrease in molecular weight and suppressing the generation of fine powder. The problem is solved by a method for producing PHA, the method comprising: (a) a step for enzymatically treating a culture solution containing PHA-containing bacterial cells to obtain a PHA aqueous suspension; (b) a step for heating the obtained aqueous suspension at (Tm-50) °C to (Tm-25) °C; (c) a step for further heating the obtained aqueous suspension at (Tm-17) °C to (Tm-2) °C; and (d) a step for cooling the obtained aqueous suspension, wherein the pH of the aqueous suspension during the heating is 2.0 to 5.0.