120 results about "Acidogenesis" patented technology

The invention discloses a system for producing short-chain volatile fatty acid through kitchen co-fermentation of sludge, and relates to the technical field of biochemical engineering and environmental engineering. Comprising a time phase construction module, a causal playback identification module, a flow field mapping correction module, a metabolism gating feedback module, a structure reconstruction steady-state module and a phase traction control module, and reconstructing a gas-liquid phase difference reference surface according to the real-time flow field response, and establishing a reference surface to calibrate an anti-phase trigger window. According to the method, gas-liquid reverse-phase accurate identification is realized through a high-precision time base line and flow field tracking, a causal chain is constructed to early warn an inverted nucleation area in advance, a steady-state energy base plane is established in combination with three-dimensional flow field correction and metabolism monitoring feedback and driving structure cooperative adjustment, and finally disturbance suppression and path self-resetting are realized through phase traction regulation. The acid production efficiency and the system stability are improved.

The application provides a pediococcus acidilactici M4 for low-moisture fermented feed and an application thereof, and belongs to the field of microbial application and feed technology. The application uses the pediococcus acidilactici M4 to prepare fermented total mixed ration. The bacteria have the characteristics of high osmosis and fast acid production, and can reduce the pH value of the fermentation system in a short time; have strong broad-spectrum bacteriostatic effect on escherichia coli, chicken white dysentery salmonella and staphylococcus aureus; and perfectly adapt to the fermentation of low-moisture total mixed ration. The strain is used as the fermentation strain of low-moisture total mixed ration, can significantly reduce the content of acid detergent fiber and neutral detergent fiber, improve the crude protein level, and then improve the digestibility of the daily ration, increase the content of probiotics such as lactic acid bacteria and yeast in the daily ration, reduce the content of pathogenic bacteria such as escherichia coli in the daily ration, and also can improve the content of volatile fatty acid in the daily ration, improve the quality of the daily ration, and improve the feeding value of the daily ration.

The application relates to the field of genetic engineering, and discloses an Aspergillus niger, a construction method and application of the Aspergillus niger, and a method for producing citric acid and / or succinic acid. Aspergillus niger The Aspergillus niger is obtained by simultaneously overexpressing alpha-amylase and aspartic protease in a starting strain. The Aspergillus niger provided by the application can realize efficient degradation and conversion of starch by synergistically overexpressing amyA genes and pepA genes, solve the technical bottleneck that the original strain is limited in growth in a starch-based fermentation system and has insufficient acid production capacity, can efficiently produce succinic acid and citric acid by taking low-cost starch as the only carbon source, significantly reduce the industrial production cost of organic acid, and promote the green and sustainable upgrading of the fermentation industry.

The application discloses a production process for preparing composite carbon source by utilizing fruit and vegetable waste resources, and comprises the following steps: raw material pretreatment, anaerobic fermentation for acid production, composite enzymolysis, deep solid-liquid separation, composite blending, and stabilization treatment; the blending carbon source liquid is pumped into the stabilization treatment tank, 0.1%-0.3% of a compounded stabilizer is added, the pH value is adjusted to 6.0-7.0, normal-temperature standing is carried out for 12-24 hours, product detection and storage are carried out, the carbon source product after the stabilization treatment is detected in terms of COD, total nitrogen, total phosphorus, the pH value and the content of small-molecule organic acid, and after each index is qualified, the product is sealed and packaged and stored in a cool and dry environment, and the application solves the technical problems of high cost, poor safety, single fruit and vegetable base carbon source component, low degradation efficiency and poor product stability of traditional carbon sources.

The invention discloses lactobacillus helveticus 5-1 separated from fermented milk and application of the lactobacillus helveticus 5-1. The lactobacillus helveticus 5-1 has the highest viable count in 12 hours at the end of the logarithmic phase; the lactobacillus helveticus 5-1 has better acid production capacity and acid resistance; the tolerance of the lactobacillus helveticus 5-1 is strong under the bile salt concentration of 0.3%; the thallus extract of the lactobacillus helveticus 5-1 has strong capability of removing DPPH (1, 1-diphenyl-2-picrylhydrazyl); the lactobacillus helveticus 5-1 is high in exopolysaccharide (EPS) production capacity. The lactobacillus helveticus 5-1 with high activity, strong oxidation resistance, excellent acid and cholate resistance and high exopolysaccharide yield is separated, screened and cultured from the traditional fermented milk in Xinjiang, so that the blank of multifunctional and high-performance autonomous strains in the prior art is filled; the application efficiency and the stability of the compound in probiotic preparations and functional foods are improved.

The application belongs to the technical field of microorganisms, and particularly relates to a halophilic bacillus, a bacterial agent and application thereof. The application provides a halophilic bacillus Halobacillus andaensis, and the preservation number is CCTCC M 20232394. The application also provides a bacterial agent containing the halophilic bacillus. The application also provides application of the halophilic bacillus or the bacterial agent in promoting plant growth under saline-alkali stress, improving plant stress resistance, improving soil, and crop production. The halophilic bacillus provided by the application has excellent saline-alkali tolerance, and can only grow under saline-alkali conditions. Meanwhile, the halophilic bacillus has growth-promoting characteristics such as salt reduction, acid production for alkali reduction, nitrogen fixation, and the like, can reduce salt damage, and improve crop yield and quality.

The invention discloses a process and a device for producing acid by coupling chemical enhanced membrane grid filtration with anaerobic fermentation, and belongs to the technical field of sewage treatment. The method comprises the following steps: enabling effluent of an aerated grit chamber of a municipal sewage treatment plant to pass through a reaction tank consisting of a coagulation tank and a flocculation tank, a filter tank and an anaerobic fermentation tank, and feeding obtained filtrate and mixed liquid into a biochemical tank for biological denitrification. The device adopting the process is used as a pretreatment process unit for biological denitrification of a biochemical pool, on one hand, the concentration of soluble COD (Chemical Oxygen Demand) in influent water of the biochemical pool can be improved, the total nitrogen removal effect can be further improved, the total nitrogen of effluent can be reduced by 2-4mg / L, and optimal preparation of resources is realized, and on the other hand, the process and the device can completely or partially replace an external carbon source, so that the environmental pollution is reduced. And considerable economic benefits are generated.

The invention provides a method and a system for evaluating acetic acid production capacity of strong-flavor Daqu, and relates to the technical field of microbial function analys.The method comprises the steps of obtaining kinetic parameters of the acetic acid production capacity of a main stress response stage representing the upper limit of the Daqu acid production capacity through pulse injection of gaseous ethanol, and then conducting pulse injection of atomized lactic acid serving as a metabolism perturbator to obtain the acetic acid production capacity of the Daqu. Obtaining kinetic parameters representing the acetic acid production capacity of the microbial system under external pressure in a metabolic disturbance recovery stage, and evaluating the acetic acid production capacity of the Luzhou-flavor Daqu based on the kinetic parameters of the acetic acid production capacity in the main stress response stage and the kinetic parameters of the acetic acid production capacity in the metabolic disturbance recovery stage. The method solves the problem of low efficiency of evaluating the acetic acid production capacity of the existing strong-flavor Daqu, and is suitable for evaluating the acetic acid production capacity of the Daqu.

The present application belongs to the field of biomass anaerobic digestion, and provides a two-phase integrated anaerobic digestion and exogenous particle recycling device.The device comprises a main reactor, an online liquid level control system, a gas collection system and a gas backflow stirring system, and the main reactor contains a hydrolysis acidification zone, an acid production buffer zone and a methane production zone.The hydrolysis acidification zone is provided with a hydrolysis acidification chamber, a stirring device, a hydrolysis acidification chamber sample discharge port and a sample inlet.The acid production buffer zone comprises an acid production buffer chamber and an improved three-way valve.The methane production zone comprises a methane production chamber sample discharge port, a gas distribution device, a methane production chamber, a PVC connecting pipe, a particle settling zone, an overflow sample discharge port, a partition, a particle settling zone sample discharge port, a baffle and a sludge discharge port.The two-phase integrated anaerobic digester in the present application separates the hydrolysis acidification zone and the methane production zone through an upper and lower layered anaerobic digestion tank, which is conducive to improving the organic load and volumetric gas production rate of the system, and the reactor structure is compact, reducing the floor area.

The invention belongs to the technical field of fermented tea beverages, and particularly discloses compound fermented Kangpu tea as well as a preparation method and application thereof. According to the compound fermented Kangpu tea, black tea water is inoculated with compound bacteria for fermentation, the compound bacteria are composed of foal bacillus medialis, saccharomyces cerevisiae and sake lactobacillus, the mass ratio of the foal bacillus medialis to the saccharomyces cerevisiae to the sake lactobacillus is 2: 2: (1-2), the preservation number of the foal bacillus medialis is WDCM No. JCM 16936, the preservation number of the saccharomyces cerevisiae is CGMCC No. 2.3973, and the preservation number of the sake lactobacillus is CGMCC No. 2.3973. The sake lactobacillus is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34016. Compared with single-bacterium or two-bacterium composite inoculated fermentation, the growth activity, the acid production capacity and the reducing sugar consumption capacity of the Kangpu tea are better, the content of flavor substances such as aldehydes and esters is improved through the GC-IMS technology, and the flavor quality of the Kangpu tea is improved.

The present application relates to the field of agricultural microorganism technology, and discloses a strain of starch-degrading Levilactobacillus brevis AM515, which has a preservation number of CGMCC No. 33115, and the strain can have a degradation or structural modification capacity on corn starch in an anaerobic silage fermentation environment with a pH of 3.5-4.2. Development and application of a strain of Levilactobacillus brevis for improving in-vitro digestibility of whole-plant corn silage starch, the provided Levilactobacillus brevis AM515 (CGMCC No. 33115) is a functional strain directly screened from a silage environment. It can not only produce acid quickly like traditional lactic acid bacteria and dominate silage fermentation, but also has the ability to survive stably in a harsh low-pH (3.5-4.2) and anaerobic silage environment, degrade the starch content in corn silage feed, and improve the in-vitro digestibility of starch. This overcomes the dual technical obstacles of the difficulty of ordinary starch-degrading bacteria to adapt to the silage environment and the insufficient starch capacity of traditional silage lactic acid bacteria.

This invention relates to the field of biotechnology, specifically disclosing a freeze-drying protection system for Enterococcus faecalis, its preparation method, and its applications. The freeze-drying protection system comprises arginine, skim milk, and glycerol, combined with low-temperature stress pretreatment. The freeze-drying protection system provided by this invention significantly increases the freeze-drying survival rate of Enterococcus faecalis L118 to 87.90±1.28%, and the survival rate remains above 90% after 28 days of storage at -20℃. The core probiotic characteristics of the freeze-dried Enterococcus faecalis L118 strain, such as acid production capacity, simulated gastric juice, and NaCl tolerance, are well preserved, providing an effective solution for preparing highly active and stable Enterococcus faecalis preparations.

The screening method comprises the following steps: S1, taking a high-temperature yeast sample, adding sterile normal saline, soaking and oscillating, taking supernate, inoculating the supernate into an acetic acid bacteria enrichment culture medium, and culturing at constant temperature to obtain enriched liquid; s2, carrying out gradient dilution on the enrichment liquid, taking a diluent with a proper concentration, coating a calcium plate isolation culture medium with the diluent, and selecting a single colony generating a transparent circle to carry out multi-generation streak purification; s3, inoculating the purified strain to an acidogenic qualitative culture medium, carrying out shake cultivation, centrifuging the fermentation broth, taking the supernatant, neutralizing the supernatant with a NaOH solution until the pH value is 7, adding a FeCl3 solution, and heating in a boiling water bath to generate a rufous flocculent precipitate which is an acidogenic bacterium; and S4, preparing bacterial suspension from the bacterial strains which are qualitatively identified as acid-producing bacteria, inoculating the bacterial suspension to a basic culture medium, and selecting the bacterial strain with the highest acid yield as a target bacterial strain. The strain with high acid yield and high stress tolerance obtained by screening provides a high-quality microbial resource for improving the quality of Maotai-flavor liquor.

The application discloses an application of astragalus polysaccharide in promoting lactic acid bacteria metabolism and improving quality and stability of fermented products, and belongs to the technical field of fermented dairy product processing. The application adds 2 ‰ to 10 ‰ of astragalus polysaccharide and sucrose in skimmed milk with respect to the mass of the skimmed milk, inoculates lactic acid bacteria starter after pasteurization, and constant-temperature fermentation is carried out until the end point to prepare fermented products. The application utilizes the dual functions of astragalus polysaccharide in promoting lactic acid bacteria metabolism and strengthening milk protein gel network to realize the synergistic improvement of quality and stability of fermented products. Experiments show that when the addition amount of astragalus polysaccharide is 4 ‰, the sensory score of the fermented product reaches 87.3, and the water holding capacity is increased by 11.95 % compared with the control group; scanning electron microscopy shows that the gel network is dense and has few pores; when the addition amount is 6 ‰, the viable cell count reaches 1.23 x 10 8 CFU / ml; and when the addition amount is 8 ‰, the texture characteristics are optimal. The application is simple in operation, suitable for industrial production, and provides a new way for the development of functional fermented dairy products.

This invention discloses a method for preparing a red mud-like soil matrix synergistically modified by agricultural and forestry solid waste and microorganisms. This method not only introduces agricultural and forestry solid waste with different carbon-to-nitrogen ratios—high carbon-to-nitrogen ratio solid waste providing a recalcitrant carbon source to maintain long-term metabolism, medium carbon-to-nitrogen ratio solid waste serving as a readily degradable carbon source to accelerate initial fermentation, and low carbon-to-nitrogen ratio solid waste supplementing nitrogen to avoid carbon-to-nitrogen imbalance—but also ensures sufficient carbon source for microorganisms to continuously produce acid and reduce alkali while avoiding excessive nitrogen loss, thus promoting efficient mineralization and humification of organic matter. Furthermore, by introducing biochar-loaded modified microorganisms, the method utilizes the organic acids secreted by microorganisms to deeply reduce alkali, and also utilizes the extracellular polymers secreted by the microorganisms to combine with red mud metal ions to form organic-mineral composite colloids, enhancing the stability of red mud aggregates. This invention features a simple process, low cost, and is suitable for widespread application in fields such as ecological restoration of red mud dumps, mine reclamation, and saline-alkali land improvement.

The invention provides a method for regulating and controlling anaerobic fermentation grading through intermediate product feedback, in the method, the stage number of hydrolysis stages is divided according to the hydrolysis characteristic of a fermentation material, the stage number of acid production stages is divided according to the acid production characteristic of a hydrolysate, a methane production stage is set as a first-stage methane production reactor, and a second-stage methane production reactor is set as a second-stage methane production reactor; grading nodes of all stages of anaerobic fermentation are provided, efficient and stable operation of multi-stage anaerobic fermentation can be achieved, and the method is simple and easy to implement.

The application discloses a preparation method of a porous sulfur mineral autotrophic denitrification material and application of the material in black and odorous water purification. The porous sulfur mineral functional material is prepared by using coral, iron-manganese oxide minerals and sulfur minerals, so that waste is reused, resources are saved, and the preparation method is simple; on the other hand, the porous sulfur mineral functional material has a large specific surface area and high activity. The high-activity porous sulfur mineral functional material can be used as an electron donor of sulfur autotrophic denitrification bacteria and denitrifying sulfur bacteria in a black and odorous water body with a low carbon-nitrogen ratio. Meanwhile, the coral, iron-manganese oxide minerals, nano zero-valent calcium and nano zero-valent iron-manganese formed by hydrogen reduction roasting, and the hydrated silicate formed by the sulfur mineral and cement improve the alkalinity in the reaction system, can neutralize the sulfur autotrophic denitrification acid production process, and realize deep purification of the black and odorous water body.

The invention provides a method for improving the anaerobic fermentation acid production effect of oxytetracycline-containing sludge by using thiosulfate pretreatment, which comprises the following steps: adding anaerobic sludge of a sewage plant into a fermentation tank as inoculated sludge, adding oxytetracycline and thiosulfate, then blowing nitrogen to drive oxygen, sealing the fermentation tank to degrade oxytetracycline through constant-temperature anaerobic fermentation, and carrying out centrifugal separation to obtain the oxytetracycline-containing sludge. The supernatant is fermentation liquor rich in volatile fatty acid; wherein the total suspended solid concentration of the inoculated sludge is 20-27 g / L, the mass concentration of the thiosulfate in the sludge is 900 mgS / L in terms of S, and the adding concentration of the oxytetracycline is 5-25 mg / L. According to the method, sludge hydrolysis is promoted, a microbial community structure is adjusted and electron transfer is accelerated by utilizing the reducibility of thiosulfate, so that the conversion rate of organic matters to VFA is increased, oxytetracycline is synchronously and efficiently removed, and a new solution is provided for antibiotic pollution control and sludge resource utilization.

The invention relates to the technical field of Baijiu brewing, and particularly discloses an acid reduction technology in the Maotai-flavor Baijiu brewing process. The method aims at solving the technical problems that in traditional Maotai-flavor liquor production, due to dependence on experience, acidity fluctuation between batches is large, and after-run resources are extensively utilized. The core improvement lies in two points: 1) in the stacking fermentation stage, the fermentation micro-ecology is adjusted and the acid production pedigree is guided to tend to be stable by controlling the mixing use proportion and time of the high-temperature yeast and implementing a sectional water replenishing process; and 2) in the distillation liquor receiving stage, the feint is creatively subjected to refined separation and collection, and the feint subjected to traditional mixed collection is separated into a'high-ester seasoning stage ', a'balanced seasoning stage' and a'low-quality tail water stage '. According to the method, complex and expensive equipment is not needed, effective regulation and control of acidity and recovery of high-value flavor substances are realized through refined management and control of existing process nodes, and the sensory quality consistency and the high-quality product rate of the base liquor are remarkably improved.

The invention relates to the technical field of food fermentation, and discloses a lactic acid bacteria strain and application thereof in dregs vinegar fermentation, the lactic acid bacteria strain involved in the invention is Lactobacillus paracasei ZPC30, is preserved in Guangdong Microbiological Culture Collection Center, has a preservation number of GDMCC NO: 67278, is preserved on November 5, 2025, is obtained by screening dregs vinegar distillers' grains, has a preservation number of GDMCC NO: 67278, has a preservation number of CGMCC NO: 67278, has a preservation number of CGMCC NO: 67278, has a preservation date of CGMCC NO: 67278, and has a preservation number of CGMCC NO: 67278, and has a preservation number of CGMCC NO: 67278. The strain has good growth, acid production and ethanol tolerance. Meanwhile, the invention provides application of the strain in dregs vinegar fermentation and product preparation, saccharified rice is inoculated with the lactic acid bacteria strain and a leavening agent according to a set inoculation sequence and inoculation amount, and dregs vinegar is obtained after standing culture, so that the product quality of the dregs vinegar can be improved by using the strain, and the production cost is reduced. And a new way is provided for development of leavening agents of dregs vinegar or other vinegar products.

The invention discloses a method for producing polyhydroxyalkanoate through microbial fermentation and application of the polyhydroxyalkanoate. The method for producing the polyhydroxyalkanoate through microbial fermentation comprises the following steps: optionally adding water into organic acid feed liquid for dilution, and then adding a complex microbial inoculant containing flavobacterium for aerobic fermentation to obtain PHA-rich fermentation thalli; the complex microbial inoculant comprises neosilurus meridionalis, nitrogen-fixing vibrio, dearomatization bacteria, flavobacterium and paracoccus; the organic acid feed liquid is obtained by carrying out acid production fermentation on a pretreated material derived from lignocellulose. According to the method, the lignocellulose is used as a raw material to replace a grain raw material to produce the organic acid under the condition of no enzymolysis. The microbial agent disclosed by the invention contains neosilurus meridionalis, dearomatization bacteria, flavobacterium, flavobacterium and paracoccus, the strains act together, the organic acid can be effectively utilized for further fermentation to generate polyhydroxyalkanoate, and the microbial agent has important economic and environmental benefits.

The application discloses a cold water fish fermentation product obtained through synergistic fermentation of a microbial composition and a preparation method thereof, and belongs to the field of microbial fermentation food. The application comprises the following steps: compounding Lactobacillus plantarum, Lactobacillus sakei and Saccharomyces cerevisiae into a microbial composition, preparing a pickling liquid from the complex microbial liquid of the three kinds of microorganisms, taking the dorsal muscle of a cold water fish as raw material, and performing pickling and drying fermentation treatment to obtain the cold water fish fermentation product. Experimental results show that inoculation of the microbial composition provided by the application can quickly produce acid to form a low pH environment, efficiently regulate protein hydrolysis, significantly shorten the fermentation period, inhibit lipid oxidation and reduce the TBARS value. The application provides a new microbial composition and fermentation method for low-salt, efficient and safe fermentation of cold water fish, and lays a foundation for improving the fermentation flavor of the cold water fish fermentation product, stabilizing the product quality and improving the product edible safety.

The present application relates to a kind of closed loop processing and utilization method of biogas residue based on gas-carbon coproduction, belong to organic solid waste processing field.Closed loop processing and utilization method includes the following steps: S1, biogas residue is mixed with raw material water, by hydrothermal carbonization technology, obtain hydrothermal carbonization wastewater and hydrothermal carbon;S2, hydrothermal carbonization wastewater is mixed with hydrothermal carbon, anaerobic fermentation, obtain biogas and fermentation product;S3, fermentation product is centrifuged, obtain supernatant, and the supernatant is recycled for S1, and solid remainder is recycled for S2;S1 biogas residue is obtained by acid production fermentation.Adopt biogas residue as raw material and carry out hydrothermal carbonization processing;Residual organic acid in the remainder after acid production fermentation, in the process of hydrothermal carbonization, residual organic acid promotes the depolymerization of not completely degraded lignocellulose, so that the performance of the obtained product hydrothermal carbon is improved.The present application can convert and utilize the effective carbon source components in hydrothermal carbonization aqueous phase byproduct, and recover the energy contained in biogas residue to the maximum extent.

The invention discloses a full-quality utilization method for continuously preparing biogas from straw biomass, and belongs to the technical field of agricultural waste resource utilization. The method comprises the following steps: firstly, cooperatively treating a lignocellulose raw material by adopting physical crushing and chemical catalysis, and establishing a hydrolysis kinetic equation for monitoring the degradation efficiency of lignocellulose; then transferring the pretreated material into an acidification reaction kettle, inoculating an acid-producing microbial agent, monitoring the VFA concentration, and regulating and controlling the VFA concentration through a reflux ratio R and stirring strength I; and finally, carrying out solid-liquid separation on the obtained material, enabling filtrate to sequentially flow into a multi-stage serial gasification tank, and regulating and controlling the specific growth rate of microorganisms. Through a continuous circulation process, all-component and all-quality utilization of biomass and collaborative prevention and control of pollutants are achieved, a sustainable process system which is short in reaction time, high in system stability, efficient in resource circulation and low in environmental load is finally formed, and the development requirements of precision and low carbon of agricultural waste resource utilization under the dual-carbon target are met.

The application relates to the field of food brewing, and discloses a brewing composition and process for preparing aged vinegar from highland barley wine lees. The raw materials of the composition include highland barley wine lees, Huangyuan aged vinegar brewing Daqu, bran, rice husk and salt. The production process comprises the following steps: firstly, mixing the wine lees, the Daqu and a first part of the bran, and controlling the conditions to carry out solid anaerobic alcohol fermentation; then, adding the remaining second part of the bran and the rice husk to carry out solid acetic acid fermentation; adding salt at the end of fermentation to seal and mature; and finally, obtaining the finished product through vinegar pouring and sterilization. Through the step-by-step addition of bran and the anaerobic-aerobic two-stage fermentation strategy, the carbon and nitrogen sources required for microbial metabolism are effectively supplemented, the problem of low acid production when directly brewing vinegar from highland barley wine lees is solved, and the utilization rate of raw materials, the physical and chemical indexes and the sensory quality of the finished product are significantly improved.

This invention relates to the field of saline-alkali land amendment technology. The invention provides a saline-alkali land amendment, its preparation method, and its application. The saline-alkali land amendment comprises the following raw materials in parts by weight: 50-60 parts of straw and livestock manure compost, 30-40 parts of lignite powder, 2-4 parts of desulfurized gypsum, 2-4 parts of citric acid gypsum, 3-4 parts of superphosphate, 3-4 parts of γ-polyglutamic acid, and 1-2 parts of a compound microbial agent solution. This invention effectively increases the organic carbon content of saline-alkali land and improves soil aggregate structure by introducing manure-based and mineral-based organic fertilizers; it enhances the calcium-increasing and sodium-removing effects of desulfurized gypsum, citric acid gypsum, and superphosphate; it introduces γ-polyglutamic acid, which has strong water retention and ion chelating capabilities, effectively fixing salts; and it introduces a compound microbial agent that not only regulates the microbial community composition of saline-alkali land but also produces acid to regulate soil pH and activate fixed phosphorus in the soil.

The invention discloses a preparation method of a nitrogen removal material by a sulfur autotrophic denitrification method. The nitrogen removal material is prepared from elemental sulfur, calcium carbonate, a binder and trace elements. According to the invention, the influence of the manufacturing process does not float on the surface of a water body like elemental sulfur, the filler can slowly release effective components in the filler according to the total nitrogen content in the water body, and meanwhile, main components of the material can participate in actual reaction and do not generate precipitates, so that a user does not need to worry about residual waste residues after actual operation; wherein calcium carbonate not only provides an inorganic carbon source for sulfur autotrophic bacteria, but also can fully neutralize acidity generated in autotrophic denitrification; the problems that elemental sulfur particles can be discharged along with water flow, sodium thiosulfate needs to be quantitatively added, and waste residues left after sulfur-containing ore reaction need to be additionally treated are effectively solved, and the adverse effect of elemental sulfur type autotrophic denitrification acid production on the reactor is also solved.

The invention discloses a rapid pickle fermentation method based on pH-responsive release bioactive peptide microcapsules, and belongs to the technical field of food fermentation. The method comprises the following steps: pretreating raw materials, putting into a jar, preparing fermentation liquor, and sealing and fermenting. The method is characterized in that pH response type active peptide microcapsules are added into the fermentation liquor; according to the microcapsule, active peptide is embedded by taking calcium alginate as a matrix, and a chitosan / calcium alginate polyelectrolyte compound film layer is formed on the surface through electrostatic self-assembly. At the initial stage of fermentation (pH gt: 5.5), the film layer structure is compact, active peptides are effectively locked, and natural succession of microbial communities is prevented from being interfered; when the fermentation is in the middle stage, the lactic acid bacteria produce acid to reduce the pH value to a critical point of 4.5-5.0, the membrane layer is protonized, swelled and collapsed, and active peptides are released to promote the proliferation of the lactic acid bacteria. According to the method, the fermentation period can be greatly shortened, rich flavors generated by metabolism of various microorganisms are reserved to the maximum extent, and the technical problem that fermentation acceleration and flavors are difficult to consider at the same time is solved.

The invention discloses a genetically engineered bacterium for producing histidine and application of the genetically engineered bacterium. The genetically engineered bacterium is obtained by inhibiting the activity of purine blocker protein PurR and improving the activity of galactose transporter protein GalP in histidine producing bacteria. The invention also discloses a method for preparing the genetically engineered bacterium, a method for producing histidine and application of the genetically engineered bacterium in histidine production. The histidine acid production level and cell growth of the strain are balanced, the histidine acid production level and acid production rate are remarkably improved, and soluble impure protein is remarkably reduced.