472 results about "Acidogenesis" patented technology

Compositions and methods are given for delayed breaking of viscoelastic surfactant gels inside formation pores, particularly for use in hydraulic fracturing. Breaking inside formation pores is accomplished without mechanical intervention or use of a second fluid. Bromate oxidizing agents are used along with selected breaking activators for the bromate breaking compounds. Useful bromate breaking activators include acid-generating breaking activators, oxidizing sulfur containing breaking activators, and reducing agent breaking activators.

The invention discloses a lactobacillus leavening agent, a preparation method thereof and a special bacterial strain. The preservation serial number of lactobacillus is CCTCC M208151. An active constituent of the lactobacillus leavening agent is the lactobacillus. The invention also discloses a method for preparing the lactobacillus leavening agent, which comprises the following steps: firstly, leavening and culturing lactobacillus plantarum SC79 CCTCC M208151 in SC79 optimal culture medium; and secondly, collecting thallus in the step 1 and then adding a protective agent to the thallus to obtain the leavening agent. The lactobacillus leavening agent has small dosage, quick acid production speed through mixing the lactobacillus leavening agent and a harvestless exopolysacchatide yoghourt bacterial strain to prepare yoghourt, better relative viscosity, elasticity, denseness and adhesiveness than that of the conventional yogurt and small syneresis sensibility of formed sticky yogurt colloids, larger water holding capacity than that the conventional yogurt and hard whey separation.

Animal or human tissue is digested to extract carbon-containing gases. The digestion process can include hydrolysis, acidogenesis, acetogenesis, and methanogenesis. The step of denitrification can be added if too much nitrogen is produced during digestion. Digestion can also include enzymes that break down the tissue in preparation for methanogenesis. The carbon-containing gas can be added onto or be incorporated throughout a natural or synthetic diamond or other gemstone. The carbon-containing gas can be added during hydrothermal synthesis to create a gemstone with the carbon-containing gas incorporated throughout.

Methods of treating a subterranean formation penetrated by a wellbore of a well, wherein the subterranean formation includes carbonate. The methods can include the following steps of: (1) optionally, fracturing the subterranean formation; (2) optionally, acidizing the subterranean formation with a Bronsted-Lowry acid; (3) treating the subterranean formation with an acid-producing microorganism, a nutrient for the microorganism, and, if needed, a suitable electron acceptor for respiration by the microorganism; (4) optionally, flushing the wellbore with a wash fluid to push the microorganism deeper into the subterranean formation and wash it away from the metal tubulars of the well; (5) preferably, shutting-in the well for a required incubation period for in-situ acid generation by the microorganism; and (6) preferably, after the shut-in, flowing back fluid from the subterranean formation into the wellbore.

The invention discloses lactobacillus plantarum CQ02-108 and application thereof to preparation of fermented sausages. The lactobacillus plantarum CQ02-108 is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 17, 2018; and the preservation number is CGMCC No. 16121. The lactobacillus plantarum CQ02-108 can be used for producing proteinase and rapidly producing acid, does not cause viscosity, produce gas, produce biological amine, produce H2O2, produce H2S and produce pigments, and also has the advantages of high-salt concentration tolerance, high acidity tolerance, inhibition of growth of escherichia coli and staphylococcus aureus, bile salt resistance and gastric juice tolerance. When the lactobacillus plantarum CQ02-108 is used for fermenting the sausages, the quantity of viable bacteria of lactic acid bacteria in the fermented sausages can be remarkably improved and the pH (Potential of Hydrogen) value of the sausages is reduced; and the growth ofharmful bacteria is inhibited and the quality of the fermented sausages can be remarkably improved.

The invention discloses a method for constructing strain Escherichia coli JM127 in high product malic acid gene project and acid producing method thereof. The constructing method mainly includes deactivating or knocking out enzyme with ability to make malic acid dehydrate and pyruvate degrade, and over-expressing enzyme regenerated by NAD. A two-stage fermentation experiment using a constructed Escherichia coli is carried out, which includes steps of: (1) inoculating activated Escherichia coli into enrichment medium, aerobically culturing to enhance biomass; (2) after treating the bacteria liquid membrane obtained by aerobic culture, introducing it into LB culture medium containing 20-100 g/L glucose and a definite content of carbonate, anaerobically fermenting to produce malic acid.

The invention discloses a method for preparing regenerated cream cheese, which comprises the following steps: (1) melting and mixing raw materials comprising natural cheese, milk fat, milk protein, emulsifying salt, carbohydrates, edible salt, stabilizer and water in addition to fermentation acid-producing yak milk type cheese; (2) low-temperature acid-adding process; (3) pasteurization; (4) homogenization process; and (5) fast cooling. The invention can overcome the defects of all aspects of the natural cream cheese process, the existing regenerated cream cheese process, the conventional regenerated cheese process and the like and provides the method for preparing the regenerated cream cheese; compared with the traditional natural cheese production process, the method is greatly simplified and has the advantages of simple production process, small equipment input and short production cycle, thereby having great advantages on the cost, leading a product to have high additional value, leading the better raw materials of the product to have relative advantages on the cost and being more realistic and convenient when in real production. Compared with the natural cheese, the senses of the prepared regenerated cream cheese are better.

The invention discloses a production method of a microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes. The production method is characterized by comprising the following steps: carrying out symbiotic assemblage screening on lactic acid bacteria and saccharomycetes separated from an Inner Mongolia dairy product kumiss to select a set of enterococcus faecium HE5 and brewer yeast J-4 with mutual promotion, wherein the viable count of the lactic acid bacteria reaches 2.88*10<9>CFU (Colony-Forming Unit)/mL when enterococcus faecium HE5 and brewer yeast J-4 screened are cultivated on an optimized skimmed milk culture medium, the viable count of the saccharomycetes reaches 3.3*10<8>CFU/mL, and double bacteria culture is better to promote the growing effect of lactic acid bacteria; and freezing and drying the cultivated and harvested double bacteria to obtain the microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes. The microbial preparation is good in symbiotic effect and strong in acid-producing capacity.

The invention discloses a microorganism bacterium agent and a preparation method thereof, and relates to a composite bacterium agent and a preparation method thereof. The composite bacterium agent can be used for efficiently removing a large amount of COD (chemical oxygen demand), ammonia and nitrogen in leather wastewater. The composite bacterium agent is prepared from mycobacterium, microbacterium, acinetobacter, pichia pastoris, lactobacillus, klebsiella oxytoca and a liquid culture medium. The preparation method comprises the following steps of 1, activating the bacteria strains of raw materials; 2, enriching and culturing; 3, mixing the bacterium strains according to a weight ratio, and adding into the liquid culture medium, so as to obtain the composite bacterium agent. The composite bacterium agent has the advantages that the biological activity is high, the growth speed is high, the leather wastewater can be treated under the low temperature condition, the removal rate of organic matter can be obviously increased, the contents of COD, ammonia and nitrogen in the leather wastewater are effectively decreased, and the effect is obvious.

The invention discloses a high-temperature-resistant Acetobacter pasteurianus strain and an application thereof in production of acetic acid through fermentation. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) and has a preservation number of CGMCC No.8189. The acetic acid bacteria disclosed by the invention can grow and generate acid at 25-45 DEG C; the strain and ethanol serving as a main raw material are used to produce acetic acid through liquid submerged fermentation, and the temperature and ventilation volume during fermentation are controlled in three stages; the concentration of acetic acid obtained after fermentation can reach 60-180g/L, and the acid conversion rate of ethanol reaches 85-101%. The production of acetic acid through fermentation is performed by using the high-temperature-resistant acetic acid bacteria, and a relatively high fermentation temperature is adopted, so that the consumption of cooling electricity and cooling water is reduced, relatively high production efficiency is achieved at the same time, and the production cost is reduced. The high-temperature-resistant acetic acid bacteria and a fermentation process thereof are suitable for production of a variety of table vinegar including food vinegar, fruit vinegar and the like through fermentation.

The invention provides bacillus subtilis which is classified and named bacillus subtilis CY-4 and is preserved in Institute of Microbiology, Chinese Academy of Sciences at No. 3 Yard, No. 1, Beichen West Road, Chaoyang District, Beijing, on May 13, 2016, the postal code is 100101, and the preservation number is CGMCC No.12447. The invention further provides application of the bacillus subtilis to kitchen wastes. The bacillus subtilis has the activities of highly-active proteases, amylases and oil hydrolases, degrades glucose-produced acid and contains thermophilic bacteria with high salt resistance and acid resistance, and through the effective combination with a carrier, the weight of the bacillus subtilis put into the kitchen wastes can be quickly and effectively reduced to 85 percent.

The invention discloses rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof. The invention provides a strain namely rhodococcus ruber YMHL-1 capable of degrading residual nicosulfuron in wastewater, and the identification shows that the strain belongs to Rhodococcus. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC in February 9th, 2015; and the preservation number is CGMCC No. 10542. The main biological characteristics are as follows: the Gram staining reaction is negative; the bacterial colony is in an orange yellow color, is in a round shape, and is opaque, the surface of the bacterial colony is rough and dry; the cell is in a sphere shape or short bar shape, does not have any flagellum, is motionless, and does not generate any spore; the strain is aerobic and chemoheterotrophic and cannot liquefy gelatin and hydrolyze starch; the enzyme contact reaction is positive, the V.P. reaction is positive, the methyl red reaction is negative; the strain cannot degrade casein, cannot reduce nitrates; and the strain can produce acids from glucose, can utilize glucose, fructose, and citrates, cannot utilize mannose, arabinose, and raffinose, and can reduce more than 90% of antibiotics in water through direct application.

The invention relates to a solid-liquid two-phase anaerobic fermentation apparatus and method. The solid-liquid two-phase anaerobic fermentation apparatus comprises a solid-producing acid fermentation reactor, a liquid-producing methane reactor, an acid-producing fermentation liquor collecting tank, a biogas slurry collecting tank, a cyclic heating device, a methane meter, a blender, a sewage pump, a liquid level sensor, a temperature sensor and an automatic control cabinet. The solid-liquid two-phase anaerobic fermentation apparatus and method has the advantages that a liquid-phase fermentation device is small in size, fast in temperature rising, good in insulation and low in energy consumption; the apparatus is small in space occupation, low in investment, and about 20% of the investment can be saved as compared with traditional wet fermentation; the apparatus and method is suitable for recycling use of organic wastes of pig manure, straw, vegetable residues, kitchen wastes, municipal wastes and the like, and the apparatus and method is water saving, free of biogas slurry discharge, free of secondary pollution and the like, and substrate conversion rate and volume biogas production are high.

The invention discloses a Lactobacillus johnsonii, and a preparation and application thereof, and discloses an optimal culture medium for the strain and a preparation method of a freeze-dried preparation of the strain. The microbial preservation number of the Lactobacillus johnsonii ZLJ010 is CGMCC No.5762. The strain is definite in hereditary basis, and has the characteristics of good growth performance and high acid production, acid resistance, heat resistance and bacterium inhibition capabilities. When being used as a feed additive for sows, the freeze-dried preparation of the strain can improve the intestinal tract health of the sows, reduce the number of harmful bacteria in feces, enhance the body immunity and increase the production performance of the sows.

The invention discloses a fermentation method for improving the yield of isoleucine. The fermentation method comprises the following steps: culturing microorganisms, fermenting, separating fermentation liquor, carrying out ion exchange on fermentation supernatant, deaminizing with a deamination tower, decolorizing with activated carbon and crystallizing by evaporating. Because the corn steep liquor which serves as a hydrolysis liquor in the culture medium and accounts for 40% of the total weight of the culture medium replaces the hydrochloric acid adopted by the traditional method to regulate the pH value, the corrosion of the hydrochloric acid to the stainless steel tank is avoided. In addition, because biotin and VB1 are used as growth factors in the process, the viscosity of the fermentation liquor can be reduced, the respiratory metabolism of the microorganisms can be promoted, and the down-stream finished product can be extracted easily. By adopting the fermentation method disclosed by the invention, a good fermentation index can be achieved, the yield of the isoleucine which is a metabolite can be improved significantly, the average yield of the isoleucine can be up to 36.9g/L and can be increased by 30% compared with that of the metabolite isoleucine obtained by adopting the original method, the conversion rate of the metabolite isoleucine can be increased by 2-4%, and the fermentation cycle can be shortened by 10 hours.

The invention relates to a process and a device of low energy consumption sewage treatment based on carbon source recovery, which belong to the technical field of sewage (waste water) treatment. A process flow comprises a rapid coagulating basin, an anaerobic dynamic membrane-bioreactor, an effluent natural reoxygenation machine, an anaerobic digestion reactor and a gas collector. High efficient flocculation of low concentration organic sewage is carried out in the rapid coagulating basin, solid-liquid separation of the low concentration organic sewage is carried out in the anaerobic dynamic membrane-bioreactor, natural reoxygenation of effluent is carried out, and water quality stably achieves the national second grade discharge standard. Simultaneously, recovered low-quality carbon sources (anaerobic sludge) enter the anaerobic digestion reactor to finally enable energy matters in the sewage to be recovered in a mode of high-quality carbon sources of volatile fatty acids (VFAs) or methane and the like through a hydrolyzing-fermenting and producing acids-producing the methane approach. By means of coupling between a sewage treatment method and an anaerobic organism treatment process, high efficient treatment of the low concentration organic sewage is achieved in a short hydraulic retention time. The high efficient treatment of the low concentration organic sewage can be carried out on the condition of normal temperature, and energy conservation and consumption reduction of the sewage treatment can be achieved to the greatest extend.

The invention provides a lactic acid bacteria starter, which contains Lactobacillusdelbrueckiisubsp.bulgaricus with a preservation number of CGMCC No. 2922 and Streptomycesthermophilus with a preservation number of CGMCC No. 2915. The lactic acid bacteria starter can perform good lactose degradation capability during a fermentation process of the acidified milk, a combination of Lactobacillusdelbrueckiisubsp.bulgaricus with the preservation number of CGMCC No. 2922 and Streptomycesthermophilus with the preservation number of CGMCC No. 2915 is used for solving the problem of beta-galactosidase vitality and acidogenesis under a conventional fermentation technology, and percent hydrolysis of lactose in an acidified milk can be ensured. The invention also provides the acidified milk prepared by using lactic acid bacteria starter, and a preparation method of the acidified milk.

The invention discloses a municipal sludge pretreatment method enhancing sludge anaerobic fermentation acid production. The method enables sludge cells to rupture, enables organic matter to be released and converted in a superior-quality mode to the most extent and remarkably increases the quantity of organic acid produced through follow-up anaerobic fermentation by jointly inputting neutral protease and ethylene diamine tetraacetic acid disodium salt (EDTA-2Na) and adjusting potential of hydrogen (pH) and reaction temperature. Test results show that by means of the municipal sludge pretreatment method, the concentration of soluble chemical oxygen demand (SCOD) in sludge is improved by 18.0-20.0 times, the concentration of polysaccharide is improved by 11.0-13.0 times, and the concentration of protein is improved by 1.5-2.5 times. The municipal sludge pretreatment method has the advantages of being moderate in reaction condition, high in efficiency, high in sludge quantity reducing degree and the like. A new method and idea is provided for the purpose of finally achieving recycling, reduction and harmlessness of excess sludge.

Belonging to the technical field of contaminated soil remediation, the invention particularly relates to an improvement method of extremely acidified mine soil. The method utilizes an improving agentand a microbial agent to change the microbial community structure of soil, especially reduce the relative abundance of iron-sulfur oxidation acid production microorganisms and inhibit metal sulfide from oxidizing and producing acid. The improving agent can be utilized for adsorption, chelation, complexing and immobilization of heavy metal ions and reduction of the biological toxicity, thus reducing the diffusion and leakage of heavy metal ions to surrounding soil or water. The improvement method provided by the invention has the advantages of low investment cost, good acidification control andheavy metal stabilization effect, substantial increase of nutrient content, no destruction of the soil structure, and easy implementation.

The invention discloses a device for improving a steam generating rate of a low-temperature waste heat recovery system for sulfuric acid, and provides a method for improving the steam generating rate of the low-temperature waste heat recovery system for sulfuric acid. According to the invention, high-temperature acid production heat of the low-temperature waste heat recovery system is used for generating secondary steam with lower pressure, the secondary steam is sent into a high-temperature absorption tower and serves as part of make-up water, latent heat of the secondary steam is brought back to the low-temperature waste heat recovery system to generate low-pressure steam with higher pressure, and the steam generating rate for low pressure steam production of the system can be increased from 0.45 t to about 0.57 t each time 1 ton of sulfuric acid is produced.

A system and method for converting organic wastes to biodegradable thermoplastic materials including polyhydroxyalkanoates is disclosed, which method includes treating the organic wastes with an acidogenic microbial population to form fermentative organic acids, and polymerization of the organic acids by PHA-producing microbial species to form PHAs. The system includes a first compartment for acidogenesis of organic wastes without oxygen, and a second compartment, for polymer synthesis by enriched cultures of species with oxygen such as R. eutropha, P. oleovorans, or mixtures thereof. The compartments are integrated with barriers that permit mass transfer of organic acids while maintaining different culture conditions in the compartments.