350 results about "Acidogenesis" patented technology

The invention relates to the technical field of processing and utilization of microorganisms and mineral resources, in particular to a rare earth extracting agent and application thereof. The rare earth extracting agent provided by the invention comprises silicon-decomposing microorganisms, acid-producing microorganisms or fermentation products thereof which are screened from rare earth mines and rare earth wastes and have good symbiotic effect, high compatibility and high activity, so that the silicon-decomposing and acid-producing efficiency is further improved, and the rare earth element extracting efficiency is high. The rare earth leaching agent provided by the invention is simple and safe in component, and solves the problems of large chemical reagent dosage, high production cost, serious environmental pollution and the like in the rare earth waste chemical leaching process. The method is suitable for popularization.

The invention discloses Klebsiella oxytoca G1-2-4 and application thereof, and particularly relates to application of the Klebsiella oxytoca G1-2-4 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.30986 in preparation of a biocontrol preparation or feed for preventing and treating aquatic diseases. The invention further discloses application of the Klebsiella oxytoca G1-2-4 in preparation of the biocontrol preparation or feed for preventing and treating the aquatic diseases and application of the Klebsiella oxytoca G1-2-4 in preparation of the biocontrol preparation or feed for preventing and treating the aquatic diseases. The invention relates to an application of klebsiella oxytoca G1-2-4 in inhibiting the growth of pseudomonas plecoglossicida and a biocontrol preparation or feed containing the klebsiella oxytoca G1-2-4 and used for preventing and treating aquatic diseases. According to the Klebsiella oxytoca cultured in an isolated mode, the disease resistance of the Klebsiella oxytoca is verified through in-vitro and in-vivo experiments, the disease resistance mechanism of the Klebsiella oxytoca is preliminarily explored, and reference is provided for research on prevention and treatment of the visceral white-spot disease of the small yellow croakers.

The invention discloses propionibacterium capable of obtaining high-yield propionic acid through compound mutation screening and a method for fermenting propionic acid by using the propionibacterium. The high-yield propionic acid strain YCBS010 (CGMCC No.31160) is obtained by carrying out compound mutagenesis on acid-producing propionibacterium CGMCC 1.2232 serving as an original strain through microwave-ultrasonic combined mutagenesis and compound normal-pressure and room-temperature plasma (ARTP) mutagenesis means. According to the invention, YPA15 is used as a starting strain, microwave and ultrasonic combined mutagenesis is carried out for 100 seconds to screen a bacterial strain with increased propionic acid yield, then ARTP mutagenesis is carried out for 90 seconds to screen out a high-yield mutant strain YCBS010, fermentation in a 1-ton tank verifies that the highest propionic acid yield reaches 65.87 g / L and the fermentation intensity is 0.73 g / L.h after fermentation is carried out for 90 hours, and the bacterial strain has better industrial fermentation potential.

The invention discloses a multi-element solid waste-based auxiliary cementing material and a preparation method thereof, the cementing material is prepared by taking steel slag, carbide slag and sintered red mud which are acidified and modified by microbial bacteria as raw materials, adding water, mixing and carbonizing; according to the invention, mineral components in the steel slag are effectively utilized, and the steel slag is modified through acid production of solid waste and microorganisms, so that a new mode of resource utilization of wastes is successfully established, and conversion from wastes to resources is realized; the carbon immobilization capacity of the modified steel slag is obviously enhanced through microbial modification, full-amount carbonization of the steel slag is achieved, compared with a traditional acid leaching carbonization method, the method is not limited to partial carbonization through filtrate any more, the preparation method is green and safe, environmental pollution and potential safety hazards caused by adopting strong acid in chemical acidification are avoided, and the method has great application prospects.

The invention belongs to the technical field of biological agents, and particularly relates to application of plant lactobacillus BD7807 in preparation of cheese. According to the cheese prepared by taking the plant lactobacillus BD7807 as a core fermentation strain, the quality and functional characteristics of the cheese are remarkably improved. In particular, the cheese exhibits a higher water binding capacity, a stronger acid producing ability, a lower pH value, a lower fat content, and a richer level of taste. The characteristics not only optimize the texture and flavor of the cheese, but also endow the cheese with higher nutritional value and health attribute. Therefore, the method has important practical significance in promoting the upgrading of cheese products and meeting the requirements of consumers on high-quality and functional dairy products, and shows a wide market application prospect.

The invention belongs to the technical field of wastewater treatment and energy recovery, and provides a regulation and control method for promoting anaerobic acid production by taking soybean molasses as a substrate through cooperation of ferroferric oxide and bamboo charcoal. Soybean molasses is used as a substrate carbon source, anaerobic activated sludge is inoculated in a medium-temperature SBR reactor, the volume ratio of reactor inflow sewage to sludge is 2: 1, ferroferric oxide and bamboo charcoal composite conductive particles which are subjected to ball milling treatment and have the concentration of 1 g / L are added to promote an anaerobic fermentation system to produce acid, and then anaerobic fermentation is carried out. The mass ratio of ferroferric oxide to bamboo charcoal in the ferroferric oxide and bamboo charcoal composite conductive particles is (1: 2)-(2: 1); monitoring the acid yield, the pH value change and the product type; and the variety and concentration of the acid-producing substance are analyzed, and the interaction of all the components in the reaction process is evaluated. The acid production rate and the total acid production amount in the anaerobic acid production process are obviously improved. And the activity of anaerobic flora is promoted. The variety distribution of acid-producing substances is obviously optimized, and the stability of the acidification process is improved.

The invention provides an anaerobic gas-feeding bacterium subjected to mutagenesis screening and application of the anaerobic gas-feeding bacterium in high-yield acid production through synthesis gas conversion. The anaerobic gas-feeding bacterium is a Clostridium sp. Strain P38 strain, and the preservation number of the anaerobic gas-feeding bacterium is CGMCC (China General Microbiological Culture Collection Center) No.46262. According to the invention, an acid-producing Clostridium sp. Strain P38 strain is obtained through ARTP mutagenesis screening, and through optimization of culture conditions, especially addition of sodium molybdate in a culture system and control of the pH value of the culture system, the maximum concentration of bacteria fermented by the anaerobic gas-feeding bacteria Clostridium sp. Strain P38 and the capability of fermentation acid production are improved, the maximum cell concentration of the strain can reach OD600nm-2. 0Abs, the maximum acetic acid yield is 5g / L, and the hexanoic acid content is 3.5 g / L.

The invention discloses a method for promoting anaerobic fermentation phosphorus release and short-chain fatty acid production of excess sludge, relates to the technical field of sludge treatment and resource utilization, and particularly relates to a method for promoting anaerobic fermentation phosphorus release and short-chain fatty acid production of excess sludge by utilizing thermal activation potassium persulfate (PMS, K2S2O8). The method comprises the following steps: mixing PMS with excess sludge, putting the mixture into a sludge pyrolysis device, carrying out thermal activation at 80 DEG C for 2 hours, then carrying out anaerobic fermentation in a sealed reactor, and filtering the fermented sludge mixed solution to obtain a phosphorus-rich supernatant. According to the method, the residual sludge is pretreated by utilizing the thermal activation PMS, so that phosphorus in the residual sludge can be enriched from a solid phase to a liquid phase, and the release efficiency of the phosphorus in the residual sludge is improved; meanwhile, organic matters in the excess sludge are decomposed and released into supernate along with the rupture of microbial cells in the excess sludge in the pretreatment process, so that the microbial acid production fermentation rate in the anaerobic fermentation process is increased, and the resource and reduction utilization of the sludge is facilitated.

The invention relates to the technical field of agricultural waste recycling, and discloses a formula and a preparation method of an amaranthus hypochondriacus and moso bamboo powder microbial fermentation feed, and the materials comprise amaranthus hypochondriacus straw, moso bamboo powder, corn flour, a mineral premix, salt and a microbial fermentation agent. After fermentation, the content of crude fibers is obviously reduced, the content of crude proteins is increased, the effect is optimal according to the proportion of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5), lignin in the moso bamboo powder is partially degraded, polysaccharide substances are released, the palatability of the feed is improved, the pH value of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5) in the optimal fermentation group is reduced to 4.2, the content of lactic acid reaches 2.3 g / kg, the total amount of VFA is increased, and the reproduction of harmful bacteria is inhibited; the reasonable proportion of the amaranthus hypochondriacus and the moso bamboo powder can balance carbon and nitrogen sources and promote synergistic fermentation of microorganisms, degradation of crude fibers in the fermentation process is related to acid production of lactic acid bacteria and cellulase secretion of bacillus, and the fermented feed can be used as a coarse feed substitute for ruminants or used in pig feed to improve intestinal health.

The invention discloses a method for promoting acid production and alcohol production through fermentation of clostridium perdali C1 gas by using a biological source carbon-based redox regulation and control medium. The preparation method comprises the following steps: obtaining biochar by adopting a high-temperature pyrolysis method, grinding and sieving to obtain a biogenic carbon-based redox regulation medium, adding the biogenic carbon-based redox regulation medium into a fermentation culture medium, inoculating clostridium perdali into a fermentation device filled with the fermentation culture medium, controlling the volume ratio of H2 to CO2 in the fermentation device to be (1.5: 1)-(2.5: 1), and carrying out anaerobic fermentation for 20-30 days to obtain the biogenic carbon-based redox regulation medium. According to the invention, a biological source carbon-based redox regulation medium is introduced into a clostridium perdali gas fermentation system, and the medium not only can enrich CO2 gas, but also can improve the mass transfer efficiency of a gaseous substrate; the method has the advantages that the method is simple and convenient to operate, extra reducing power is provided for a fermentation system, electron transfer is mediated, the CO2 utilization rate of the strain and the fermentation product generation rate are remarkably increased, secondary resource utilization of solid waste biomass is achieved, C1 gas is effectively converted into high-value chemicals, and a green, economical and sustainable development road is provided for conversion of CO2.

The invention relates to a method for enriching anaerobic fermentation acid-producing microbial communities, a flora and application of the flora, belonging to the field of biological fermentation. The method comprises an F1 stage, an F2 stage and an F3 stage, and the organic load of the straw is gradually increased in the three stages until the straw is stably operated in the F3 stage. Wherein the stage F1 is a reactor starting stage, and the solid content and the load are low. After the acid production of the F2 is stable, the straw proportion in the material is increased, the C / N ratio is increased, and the material enters the F3 stage, so that the flora adapts to the straw material with the high C / N ratio. According to the method disclosed by the invention, the microbial flora capable of efficiently producing the SCCAs by utilizing the straw material is obtained. The medium-temperature flora and the high-temperature flora can generate SCCAs close to 20 g / L by utilizing the straw with the initial solid content of 5%, and the method has a good application prospect.

The invention belongs to the technical field of microorganisms, and particularly relates to halophilous bacillus, a microbial agent and application of the halophilous bacillus. The invention provides bacillus halophilous andaensis, and the preservation number of the bacillus halophilous andaensis is CCTCC (China Center for Type Culture Collection) M 20232394. The invention further provides a microbial inoculum which comprises the halophilous bacillus. The invention further provides application of the halophilous bacillus or the microbial inoculum to promotion of plant growth under saline-alkali stress, improvement of plant stress resistance, soil improvement and crop production. The halophilous bacillus provided by the invention has excellent saline-alkaline tolerance, can only grow under saline-alkaline conditions, also has the growth promoting characteristics of reducing salt, producing acid, reducing alkali, fixing nitrogen and the like, and can relieve the salt damage phenomenon and improve the crop yield and quality.

The invention relates to the field of microorganisms, and provides an acid-producing propionibacterium BL8 with hypoglycemic and anti-aging functions and application of the acid-producing propionibacterium BL8, the acid-producing propionibacterium BL8 is preserved in China General Microbiological Culture Collection Center on December 20, 2024, and the preservation number is CGMCC No. 33134. The acid-producing propionibacterium BL8 disclosed by the invention can be used for inhibiting the activity of DPP4, promoting insulin secretion of INS-1, and inhibiting the activity of acetylcholin esterase and excellent cellulase activity. The blood sugar can be reduced; the memory is improved, and the Alzheimer disease is prevented and relieved; and the traditional Chinese medicine composition has relatively high research and application values in the aspects of reducing blood sugar and resisting aging.

The invention relates to the technical field of microorganisms, in particular to a strain of Lactiplanilla pentosuses ZF634 and application of the strain of Lactiplanilla pentosuses ZF634 and the application of the strain of Lactiplanilla pentosuses ZF634. The lactobacillus pentosus strain ZF634 is obtained through screening and still has efficient acid production capacity in a bean-based culture medium, and the bean curd coagulator prepared from fermentation liquor of the lactobacillus pentosus strain ZF634 has the function of enhancing aroma and flavor; the beany flavor of the prepared sour slurry bean curd is obviously reduced, and the sour slurry bean curd also has special milk flavor; the protein hydrolysis degree and flavor precursor substance accumulation of the prepared fermented bean curd embryo are far superior to those of a commercial sour slurry water sample, the content of fresh and sweet amino acids and organic acids is remarkably increased, the fresh and sweet taste of the fermented bean curd is improved, the taste is softer and more harmonious, the sensory quality of the final product is broken through, and the aroma, the taste and the texture are remarkably improved.

The invention relates to a method for extracting rare earth elements based on a biological separation method, and relates to the technical field of rare earth element separation and extraction. In order to solve the problems of high pollution and poor selectivity of a traditional chemical method and low adsorption capacity, insufficient stability and the like of a biotechnology, the rare earth ion adsorbent is prepared by screening microbial strains or biomolecules with high affinity, utilizing the specific adsorption or metabolic acid production dissolution effect of surface functional groups of the microbial strains or biomolecules on rare earth ions and combining biological material modification and a combined process. And efficient enrichment and selective separation of target elements in low-grade rare earth ore, multi-metal complex ore and rare earth-containing wastewater are realized. According to the technology, key parameter regulation and control are optimized through a process kinetic model, energy consumption and environmental pollution are reduced, meanwhile, the rare earth recovery purity and process stability are improved, and an innovative solution is provided for green and sustainable development of rare earth resources.

The invention relates to the technical field of wastewater treatment or water quality purification, in particular to a method for synchronously culturing ammonia oxidizing bacteria and nitrite oxidizing bacteria, which comprises the following steps: selecting an inorganic material which is large in specific surface area and can quickly neutralize and release alkalinity as a nitrifying bacteria attachment carrier and an alkalinity supplement / pH regulator; the carrier is dissolved through nitration acid production, carbon dioxide and alkalinity are released, a carbon source and alkalinity required by growth are provided for nitrifying bacteria, and propagation of the nitrifying bacteria is promoted. Ammonia oxidizing bacteria and nitrite oxidizing bacteria are cultured synchronously by controlling the growth speed of the two bacteria in the culture process. By controlling the ammonia nitrogen concentration and nitrite concentration of the effluent at the end of culture to be lower than 50mg / L, sufficient time is provided for nitrite oxidizing bacteria to decompose an intermediate product nitrite, so that the purpose of simultaneously culturing AOB and NOB is achieved.

The present invention discloses a method for improving the methane production and purity in the anaerobic fermentation of straw-like raw materials, which comprises the following steps: S1. Pretreatment; S2. Inoculating the pretreated initial liquid material with hydrogen-producing and acid-producing microorganisms, and carrying out anaerobic fermentation by means of magnetic field strengthening and external circulation; S31. Collecting the fermentation gas in step S2, preparing nano-bubble water, then adding the pretreated initial liquid material, inoculating hydrogenophilic methanogenic microorganisms, and carrying out anaerobic fermentation to produce methane; S32. Collecting the fermentation liquid in step S2, inoculating acetic acidophilic methanogenic microorganisms, and carrying out anaerobic fermentation to produce methane by means of magnetic field strengthening and external circulation. This application separately uses acid-producing substrates and hydrogen to separate the methanogenesis stage into two independent pathways of acidophilic methanogenesis and hydrogenophilic methanogenesis. Combining magnetic field strengthening and nano-bubble technology, it improves the organic matter degradation effect, hydrogen-producing and acid-producing effect, as well as the utilization efficiency of hydrogen and acid-producing substrates, increases the methane production and purity, and shortens the entire fermentation cycle.

The invention discloses a urease-producing microorganism induced calcium carbonate deposition solution for foundation reinforcement and a preparation method and application thereof, and belongs to the technical field of foundation reinforcement. The urease-producing microorganism induced calcium carbonate deposition solution comprises a bacterial solution, a cementing solution and a sodium citrate solution, the bacterial liquid comprises bacteria capable of generating urease and citrobacter freundii. Sodium citrate in the urease-producing microorganism induced calcium carbonate deposition solution can chelate free calcium ions in advance, the concentration of the free calcium ions is reduced, the risk of precipitation at an injection port in the single-phase injection process is eliminated, and the solution can smoothly permeate into a deep layer; citrobacter freundii produces acid through metabolism and utilizes citric acid radicals through metabolism to ensure gradual release of calcium ions, so that uniform deposition of calcium carbonate is triggered. Through the synergistic effect of sodium citrate chelated calcium ions and citrobacter freundii metabolism, the solution can be uniformly distributed in the soil sample, and the problem of uniformity of single-phase perfusion is successfully solved.

The invention relates to a method for producing acid and improving dehydration performance through anaerobic fermentation of paper waste and multisource organic waste, which comprises the following steps: S1, settling multisource organic solid waste, removing supernatant, and adding into an anaerobic fermentation reactor; s2, crushing paper wastes, adding the crushed paper wastes into the anaerobic fermentation reactor in the step S1, and mixing the crushed paper wastes with the multi-source organic solid wastes; s3, removing oxygen in the anaerobic fermentation reactor, and performing sealed constant-temperature fermentation; and S4, product separation. Compared with the prior art, the method disclosed by the invention has the advantages that the reaction period is greatly shortened compared with the conventional anaerobic digestion method, and the organic acid in the generated supernate is more easily recycled than methane; by adding the paper wastes, the dehydration performance of the organic solid wastes can be improved, and the stability of the fermentation liquor can be adjusted; for paper wastes such as waste tissues and waste paper meal boxes which are difficult to directly recycle due to organic pollution, the method is an important way for realizing carbon reduction and recycling.

The invention provides a microbial agent for producing acid through fermentation of organic waste and application of the microbial agent, and relates to the technical field of biology, the microbial agent contains Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus, and the microbial agent is prepared from the following raw materials: Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus. Studies find that acetobacter burgii, clostridium cow dung and eubacterium mucilaginosus complement each other in the anaerobic fermentation process, the degradation efficiency of organic matters such as carbohydrates and proteins in the organic wastewater / perishable garbage is improved, the yield of volatile fatty acids is remarkably increased, meanwhile, the emission of CO2 is reduced, and the environmental pollution is reduced. The method has the application prospects of organic wastewater / perishable garbage treatment and recycling. When the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the fermented mixed acid liquid is rich in VFA and can be used as an organic carbon source, and the additional value is higher; when the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the operation is simple and convenient, the cost is low, biogas slurry treatment links and cost are reduced, the added value of products can be improved, and the acid-producing bacterium agent has an engineering application prospect.

The invention discloses a controlled-release fertilizer containing microorganisms and fermentation products thereof as well as a preparation process and application of the controlled-release fertilizer, and belongs to the technical field of bio-organic fertilizers. According to the invention, pediococcus acidilactici, klebsiella oxytoca and pseudomonas sp. With high efficient growth-promoting effects are screened, and the active strains and fermentation products thereof act on soil together to fix nitrogen, solubilize phosphorus and solubilize potassium, so that continuous nutrient supply is provided for crops. A fermentation product obtained by mixing the three strains according to the equal proportion is added, and active substances such as organic acid and polysaccharide are efficiently accumulated in fermentation liquor. The composite carrier composed of starch, humic acid and bentonite is added, trace elements are added, the stress resistance inducer is added, all the raw materials act together, the growth of tobacco is promoted, the use amount of chemical fertilizer is reduced, the content of potassium in tobacco leaves is increased, the quality of the tobacco leaves is improved, and the defects that an existing biological fertilizer is not high in specificity and not obvious in use effect are overcome.

The invention provides a probiotic mixed fermentation method for enhancing a synergistic effect. Lactobacillus plantarum, bifidobacterium longum and clostridium tyrobutyricum are inoculated according to a volume ratio, and a pH-triggered two-stage fermentation process is adopted: the first stage is micro-aerobic to drive lactic acid bacteria to quickly produce acid, and the second stage is anaerobic to directionally improve butyric acid synthesis by pulse feeding of inulin-cysteine sustained-release particles. Physical isolation of flora is realized by using a double-layer gradient carrier, lactic acid bacteria are immobilized by sodium alginate-gelatin gel on the outer layer to form an acid barrier, and bifidobacterium and butyric acid producing bacteria are co-immobilized by chitosan microspheres on the inner layer to construct an anaerobic microenvironment. The technology breaks through the competitive inhibition bottleneck of multi-strain fermentation, the total number of viable bacteria is increased by 150% compared with a traditional technology, the yield of butyric acid is increased by 355%, and the technology is suitable for large-scale production of functional food and pharmaceutical preparations.

The invention discloses lactobacillus plantarum WL05 capable of efficiently degrading ethanol and application of the lactobacillus plantarum WL05, and belongs to the technical field of biology. The bacterial strain is lactobacillus plantarum WL05, the bacterial strain is preserved in the China General Microbiological Culture Collection Center on July 8, 2024, and the preservation number is CGMCC No.31202. The lactobacillus plantarum WL05 has relatively high acid production capacity and activity, and the lactobacillus plantarum WL05 is preserved in the China General Microbiological Culture Collection Center on July 8, 2024. The acid resistance and cholate resistance are high; after the lactobacillus plantarum WL05 is fermented in an MRS culture medium containing 10% ethanol, the ethanol degradation rate can reach 42.5%, and the ethanol dehydrogenase activation rate is 43.53%; the fermentation liquor is inoculated into a lalang grass rhizome-reed rhizome matrix culture medium, and after fermentation, the measured ethanol degradation rate is 63.9%, and the activation rate of ethanol dehydrogenase in the fermentation liquor is 66.5%. The strain has strong reducing ability and antibacterial ability at the same time. The ethanol degradation rate of the beverage prepared based on the strain can reach 32%. The product can be used for preparing functional food for dispelling effects of alcohol and protecting liver, and has high application value in the field of functional food.

The invention discloses a micro-ecological flora in-situ screening and anaerobic acid production enrichment method and device based on selective metal ion removal, belongs to the field of anaerobic treatment of high-concentration organic wastes, and solves the problems of coordinating the flora relationship of the organic wastes and improving the enrichment efficiency of anaerobic produced short-chain fatty acids. The method comprises the following steps: gradually removing soluble Fe, Co, Ni, Mg and Ca in an exchangeable state and a carbonate binding state from organic wastes by using a filler loaded with chelating groups, creating a multivalent metal ion deficiency environment, and stresses the evolution of a micro-ecosystem by using the selectivity dependence difference of acid-producing fermentation flora and methanogenic flora on metal ions, so as to achieve the purpose of improving the yield of the organic wastes. The interaction relationship between the multivalent metal and the acidogenic fermentation flora and methanogenic flora is regulated and controlled, the flora screening effect is exerted, the acidogenic fermentation flora is enriched, the methanogenic flora is inhibited, and the acidogenic fermentation flora is promoted to become dominant bacteria for mass propagation and proliferation, so that the acidogenic reaction is improved and the methanogenic reaction is inhibited; the efficiency improvement and the lossless enrichment of anaerobic acid production of the high-concentration organic waste are realized.

The invention belongs to the technical field of anaerobic acid production, and discloses a method for increasing the yield of volatile fatty acid in a straw anaerobic hydrolysis process, which comprises the following steps of: filling straws into an anaerobic reactor, adding oxygen micro-nano bubble water and anaerobic inoculum, blowing headspace for 1 minute by using nitrogen to keep an anaerobic state, the mass ratio of the straw to the oxygen micro-nano bubble water is (1: 8)-(1: 15), and the oxygen micro-nano bubble water is obtained by filling 20 L of deionized water into a water tank, introducing oxygen according to the flow rate of 1 L / min and operating for 15 minutes. According to the method, oxygen micro-nano bubble water and anaerobic inoculation are jointly used in the straw, nutrient substance exchange and transfer between microorganisms and the external environment are accelerated, the microbial activity can be improved, release of hydrolase is promoted, the hydrolytic acidification process of the straw is accelerated, the organic matter decomposition rate of the microorganisms is increased, and the yield of the straw is increased. The yield of the volatile fatty acid is improved.

The invention discloses a system for producing short-chain volatile fatty acid through kitchen co-fermentation of sludge, and relates to the technical field of biochemical engineering and environmental engineering. Comprising a time phase construction module, a causal playback identification module, a flow field mapping correction module, a metabolism gating feedback module, a structure reconstruction steady-state module and a phase traction control module, and reconstructing a gas-liquid phase difference reference surface according to the real-time flow field response, and establishing a reference surface to calibrate an anti-phase trigger window. According to the method, gas-liquid reverse-phase accurate identification is realized through a high-precision time base line and flow field tracking, a causal chain is constructed to early warn an inverted nucleation area in advance, a steady-state energy base plane is established in combination with three-dimensional flow field correction and metabolism monitoring feedback and driving structure cooperative adjustment, and finally disturbance suppression and path self-resetting are realized through phase traction regulation. The acid production efficiency and the system stability are improved.

The invention relates to the field of microorganisms, and discloses lactobacillus paracasei and application thereof. The strain can degrade cholesterol and inhibit the growth and reproduction of streptococcus mutans; in addition, the strain not only has relatively strong acid production capacity, but also can ferment butter under a low-temperature condition, so that the butter with strong and harmonious flavor is obtained.

The invention provides a preparation method of a compound microbial agent and a pasture fermented feed, and belongs to the technical field of feed fermentation. The complex microbial inoculant disclosed by the invention comprises lactobacillus acidophilus, bacillus siamensis, bacillus amyloliquefaciens and pichia kluyveri. The lactobacillus acidophilus can produce acid and resist acid, and has better antibacterial performance; the bacillus siamensis can degrade lignin and reduce the content of neutral detergent fibers and acid detergent fibers in the fermented feed; the bacillus amyloliquefaciens produces cellulase and amylase with higher activity; the pichia kluyveri can increase the content of crude protein in the fermented feed and improve the nutritional performance of the feed. The four strains have a synergistic effect and jointly ferment forage feed, infectious microbes can be effectively inhibited, the fermented forage feed has excellent color, smell and texture, the palatability of the feed is improved, the appetite of animals is stimulated, and a good growth promoting effect is achieved; the fermented forage feed disclosed by the invention is high in nutritional quality and relatively high in feed utilization rate.

The invention provides a strain for producing L-2-aminobutyric acid as well as a construction method and application of the strain. According to the strain, E.coli W3110 is taken as a chassis strain, tdh, rhtA and ilvE genes are knocked out, the transcriptional levels of ppc, aspC, thrAfbrBC, ilvAfbr, tdcB, yaS and pntAB genes are up-regulated, an ilvI gene is weakened, bcd and gdh genes derived from bacillus subtilis B.subtilis 168 and a cysK gene derived from Corynebacterium glutamicum K051 are introduced in a heterologous manner, and the L-2-aminobutyric acid producing strain is obtained through screening and screening. The strain does not contain plasmids, is stable in heredity, does not need induction, does not need to add resistant substances, efficiently synthesizes L-2-aminobutyric acid from the beginning by taking glucose as a substrate, and has good L-2-aminobutyric acid synthesis capability, stable performance and high acid production efficiency.

The invention relates to the technical field of probiotic development and utilization, in particular to a goose source Weissella cibaria F11 and application thereof. The Weissella cibarius F11 is preserved in the China General Microbiological Culture Collection Center on July 11, 2025, and the preservation number is CGMCC NO.35211. The Weissella cibarius F11 has the characteristics of good acid resistance and cholate resistance, and can be used for preparing the acid-resistant and cholate-resistant strain. And the bacillus subtilis has a good inhibition effect on pathogenic bacteria such as escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and salmonella typhimurium, and also has stable acid production capacity and good safety. The Weissella sinus F11 provided by the invention meets the probiotic characteristic standard, the antibiotic resistance spectrum of the Weissella sinus F11 meets the feed probiotic safety standard, the growth of goslings can be remarkably promoted, the death rate and diarrhea occurrence rate of the goslings can be remarkably reduced, and the weissella sinus F11 can be used for preparing feed probiotics. The development potential in the fields of preparation of bacteriostatic and antibacterial drugs, probiotics for poultry feed, feed additives, poultry feed and the like is huge.