292 results about "Acidogenesis" patented technology

The invention belongs to the technical field of wastewater treatment and energy recovery, and provides a regulation and control method for promoting anaerobic acid production by taking soybean molasses as a substrate through cooperation of ferroferric oxide and bamboo charcoal. Soybean molasses is used as a substrate carbon source, anaerobic activated sludge is inoculated in a medium-temperature SBR reactor, the volume ratio of reactor inflow sewage to sludge is 2: 1, ferroferric oxide and bamboo charcoal composite conductive particles which are subjected to ball milling treatment and have the concentration of 1 g / L are added to promote an anaerobic fermentation system to produce acid, and then anaerobic fermentation is carried out. The mass ratio of ferroferric oxide to bamboo charcoal in the ferroferric oxide and bamboo charcoal composite conductive particles is (1: 2)-(2: 1); monitoring the acid yield, the pH value change and the product type; and the variety and concentration of the acid-producing substance are analyzed, and the interaction of all the components in the reaction process is evaluated. The acid production rate and the total acid production amount in the anaerobic acid production process are obviously improved. And the activity of anaerobic flora is promoted. The variety distribution of acid-producing substances is obviously optimized, and the stability of the acidification process is improved.

The invention relates to the technical field of agricultural waste recycling, and discloses a formula and a preparation method of an amaranthus hypochondriacus and moso bamboo powder microbial fermentation feed, and the materials comprise amaranthus hypochondriacus straw, moso bamboo powder, corn flour, a mineral premix, salt and a microbial fermentation agent. After fermentation, the content of crude fibers is obviously reduced, the content of crude proteins is increased, the effect is optimal according to the proportion of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5), lignin in the moso bamboo powder is partially degraded, polysaccharide substances are released, the palatability of the feed is improved, the pH value of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5) in the optimal fermentation group is reduced to 4.2, the content of lactic acid reaches 2.3 g / kg, the total amount of VFA is increased, and the reproduction of harmful bacteria is inhibited; the reasonable proportion of the amaranthus hypochondriacus and the moso bamboo powder can balance carbon and nitrogen sources and promote synergistic fermentation of microorganisms, degradation of crude fibers in the fermentation process is related to acid production of lactic acid bacteria and cellulase secretion of bacillus, and the fermented feed can be used as a coarse feed substitute for ruminants or used in pig feed to improve intestinal health.

The invention discloses a method for promoting acid production and alcohol production through fermentation of clostridium perdali C1 gas by using a biological source carbon-based redox regulation and control medium. The preparation method comprises the following steps: obtaining biochar by adopting a high-temperature pyrolysis method, grinding and sieving to obtain a biogenic carbon-based redox regulation medium, adding the biogenic carbon-based redox regulation medium into a fermentation culture medium, inoculating clostridium perdali into a fermentation device filled with the fermentation culture medium, controlling the volume ratio of H2 to CO2 in the fermentation device to be (1.5: 1)-(2.5: 1), and carrying out anaerobic fermentation for 20-30 days to obtain the biogenic carbon-based redox regulation medium. According to the invention, a biological source carbon-based redox regulation medium is introduced into a clostridium perdali gas fermentation system, and the medium not only can enrich CO2 gas, but also can improve the mass transfer efficiency of a gaseous substrate; the method has the advantages that the method is simple and convenient to operate, extra reducing power is provided for a fermentation system, electron transfer is mediated, the CO2 utilization rate of the strain and the fermentation product generation rate are remarkably increased, secondary resource utilization of solid waste biomass is achieved, C1 gas is effectively converted into high-value chemicals, and a green, economical and sustainable development road is provided for conversion of CO2.

The invention relates to the field of microorganisms, and provides an acid-producing propionibacterium BL8 with hypoglycemic and anti-aging functions and application of the acid-producing propionibacterium BL8, the acid-producing propionibacterium BL8 is preserved in China General Microbiological Culture Collection Center on December 20, 2024, and the preservation number is CGMCC No. 33134. The acid-producing propionibacterium BL8 disclosed by the invention can be used for inhibiting the activity of DPP4, promoting insulin secretion of INS-1, and inhibiting the activity of acetylcholin esterase and excellent cellulase activity. The blood sugar can be reduced; the memory is improved, and the Alzheimer disease is prevented and relieved; and the traditional Chinese medicine composition has relatively high research and application values in the aspects of reducing blood sugar and resisting aging.

The invention relates to the technical field of microorganisms, in particular to a strain of Lactiplanilla pentosuses ZF634 and application of the strain of Lactiplanilla pentosuses ZF634 and the application of the strain of Lactiplanilla pentosuses ZF634. The lactobacillus pentosus strain ZF634 is obtained through screening and still has efficient acid production capacity in a bean-based culture medium, and the bean curd coagulator prepared from fermentation liquor of the lactobacillus pentosus strain ZF634 has the function of enhancing aroma and flavor; the beany flavor of the prepared sour slurry bean curd is obviously reduced, and the sour slurry bean curd also has special milk flavor; the protein hydrolysis degree and flavor precursor substance accumulation of the prepared fermented bean curd embryo are far superior to those of a commercial sour slurry water sample, the content of fresh and sweet amino acids and organic acids is remarkably increased, the fresh and sweet taste of the fermented bean curd is improved, the taste is softer and more harmonious, the sensory quality of the final product is broken through, and the aroma, the taste and the texture are remarkably improved.

The invention relates to a method for extracting rare earth elements based on a biological separation method, and relates to the technical field of rare earth element separation and extraction. In order to solve the problems of high pollution and poor selectivity of a traditional chemical method and low adsorption capacity, insufficient stability and the like of a biotechnology, the rare earth ion adsorbent is prepared by screening microbial strains or biomolecules with high affinity, utilizing the specific adsorption or metabolic acid production dissolution effect of surface functional groups of the microbial strains or biomolecules on rare earth ions and combining biological material modification and a combined process. And efficient enrichment and selective separation of target elements in low-grade rare earth ore, multi-metal complex ore and rare earth-containing wastewater are realized. According to the technology, key parameter regulation and control are optimized through a process kinetic model, energy consumption and environmental pollution are reduced, meanwhile, the rare earth recovery purity and process stability are improved, and an innovative solution is provided for green and sustainable development of rare earth resources.

The invention relates to the technical field of wastewater treatment or water quality purification, in particular to a method for synchronously culturing ammonia oxidizing bacteria and nitrite oxidizing bacteria, which comprises the following steps: selecting an inorganic material which is large in specific surface area and can quickly neutralize and release alkalinity as a nitrifying bacteria attachment carrier and an alkalinity supplement / pH regulator; the carrier is dissolved through nitration acid production, carbon dioxide and alkalinity are released, a carbon source and alkalinity required by growth are provided for nitrifying bacteria, and propagation of the nitrifying bacteria is promoted. Ammonia oxidizing bacteria and nitrite oxidizing bacteria are cultured synchronously by controlling the growth speed of the two bacteria in the culture process. By controlling the ammonia nitrogen concentration and nitrite concentration of the effluent at the end of culture to be lower than 50mg / L, sufficient time is provided for nitrite oxidizing bacteria to decompose an intermediate product nitrite, so that the purpose of simultaneously culturing AOB and NOB is achieved.

The present invention discloses a method for improving the methane production and purity in the anaerobic fermentation of straw-like raw materials, which comprises the following steps: S1. Pretreatment; S2. Inoculating the pretreated initial liquid material with hydrogen-producing and acid-producing microorganisms, and carrying out anaerobic fermentation by means of magnetic field strengthening and external circulation; S31. Collecting the fermentation gas in step S2, preparing nano-bubble water, then adding the pretreated initial liquid material, inoculating hydrogenophilic methanogenic microorganisms, and carrying out anaerobic fermentation to produce methane; S32. Collecting the fermentation liquid in step S2, inoculating acetic acidophilic methanogenic microorganisms, and carrying out anaerobic fermentation to produce methane by means of magnetic field strengthening and external circulation. This application separately uses acid-producing substrates and hydrogen to separate the methanogenesis stage into two independent pathways of acidophilic methanogenesis and hydrogenophilic methanogenesis. Combining magnetic field strengthening and nano-bubble technology, it improves the organic matter degradation effect, hydrogen-producing and acid-producing effect, as well as the utilization efficiency of hydrogen and acid-producing substrates, increases the methane production and purity, and shortens the entire fermentation cycle.

The invention discloses a urease-producing microorganism induced calcium carbonate deposition solution for foundation reinforcement and a preparation method and application thereof, and belongs to the technical field of foundation reinforcement. The urease-producing microorganism induced calcium carbonate deposition solution comprises a bacterial solution, a cementing solution and a sodium citrate solution, the bacterial liquid comprises bacteria capable of generating urease and citrobacter freundii. Sodium citrate in the urease-producing microorganism induced calcium carbonate deposition solution can chelate free calcium ions in advance, the concentration of the free calcium ions is reduced, the risk of precipitation at an injection port in the single-phase injection process is eliminated, and the solution can smoothly permeate into a deep layer; citrobacter freundii produces acid through metabolism and utilizes citric acid radicals through metabolism to ensure gradual release of calcium ions, so that uniform deposition of calcium carbonate is triggered. Through the synergistic effect of sodium citrate chelated calcium ions and citrobacter freundii metabolism, the solution can be uniformly distributed in the soil sample, and the problem of uniformity of single-phase perfusion is successfully solved.

The invention provides a microbial agent for producing acid through fermentation of organic waste and application of the microbial agent, and relates to the technical field of biology, the microbial agent contains Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus, and the microbial agent is prepared from the following raw materials: Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus. Studies find that acetobacter burgii, clostridium cow dung and eubacterium mucilaginosus complement each other in the anaerobic fermentation process, the degradation efficiency of organic matters such as carbohydrates and proteins in the organic wastewater / perishable garbage is improved, the yield of volatile fatty acids is remarkably increased, meanwhile, the emission of CO2 is reduced, and the environmental pollution is reduced. The method has the application prospects of organic wastewater / perishable garbage treatment and recycling. When the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the fermented mixed acid liquid is rich in VFA and can be used as an organic carbon source, and the additional value is higher; when the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the operation is simple and convenient, the cost is low, biogas slurry treatment links and cost are reduced, the added value of products can be improved, and the acid-producing bacterium agent has an engineering application prospect.

The invention discloses a controlled-release fertilizer containing microorganisms and fermentation products thereof as well as a preparation process and application of the controlled-release fertilizer, and belongs to the technical field of bio-organic fertilizers. According to the invention, pediococcus acidilactici, klebsiella oxytoca and pseudomonas sp. With high efficient growth-promoting effects are screened, and the active strains and fermentation products thereof act on soil together to fix nitrogen, solubilize phosphorus and solubilize potassium, so that continuous nutrient supply is provided for crops. A fermentation product obtained by mixing the three strains according to the equal proportion is added, and active substances such as organic acid and polysaccharide are efficiently accumulated in fermentation liquor. The composite carrier composed of starch, humic acid and bentonite is added, trace elements are added, the stress resistance inducer is added, all the raw materials act together, the growth of tobacco is promoted, the use amount of chemical fertilizer is reduced, the content of potassium in tobacco leaves is increased, the quality of the tobacco leaves is improved, and the defects that an existing biological fertilizer is not high in specificity and not obvious in use effect are overcome.

The invention provides a probiotic mixed fermentation method for enhancing a synergistic effect. Lactobacillus plantarum, bifidobacterium longum and clostridium tyrobutyricum are inoculated according to a volume ratio, and a pH-triggered two-stage fermentation process is adopted: the first stage is micro-aerobic to drive lactic acid bacteria to quickly produce acid, and the second stage is anaerobic to directionally improve butyric acid synthesis by pulse feeding of inulin-cysteine sustained-release particles. Physical isolation of flora is realized by using a double-layer gradient carrier, lactic acid bacteria are immobilized by sodium alginate-gelatin gel on the outer layer to form an acid barrier, and bifidobacterium and butyric acid producing bacteria are co-immobilized by chitosan microspheres on the inner layer to construct an anaerobic microenvironment. The technology breaks through the competitive inhibition bottleneck of multi-strain fermentation, the total number of viable bacteria is increased by 150% compared with a traditional technology, the yield of butyric acid is increased by 355%, and the technology is suitable for large-scale production of functional food and pharmaceutical preparations.

The invention discloses a system for producing short-chain volatile fatty acid through kitchen co-fermentation of sludge, and relates to the technical field of biochemical engineering and environmental engineering. Comprising a time phase construction module, a causal playback identification module, a flow field mapping correction module, a metabolism gating feedback module, a structure reconstruction steady-state module and a phase traction control module, and reconstructing a gas-liquid phase difference reference surface according to the real-time flow field response, and establishing a reference surface to calibrate an anti-phase trigger window. According to the method, gas-liquid reverse-phase accurate identification is realized through a high-precision time base line and flow field tracking, a causal chain is constructed to early warn an inverted nucleation area in advance, a steady-state energy base plane is established in combination with three-dimensional flow field correction and metabolism monitoring feedback and driving structure cooperative adjustment, and finally disturbance suppression and path self-resetting are realized through phase traction regulation. The acid production efficiency and the system stability are improved.

The invention provides a preparation method of a compound microbial agent and a pasture fermented feed, and belongs to the technical field of feed fermentation. The complex microbial inoculant disclosed by the invention comprises lactobacillus acidophilus, bacillus siamensis, bacillus amyloliquefaciens and pichia kluyveri. The lactobacillus acidophilus can produce acid and resist acid, and has better antibacterial performance; the bacillus siamensis can degrade lignin and reduce the content of neutral detergent fibers and acid detergent fibers in the fermented feed; the bacillus amyloliquefaciens produces cellulase and amylase with higher activity; the pichia kluyveri can increase the content of crude protein in the fermented feed and improve the nutritional performance of the feed. The four strains have a synergistic effect and jointly ferment forage feed, infectious microbes can be effectively inhibited, the fermented forage feed has excellent color, smell and texture, the palatability of the feed is improved, the appetite of animals is stimulated, and a good growth promoting effect is achieved; the fermented forage feed disclosed by the invention is high in nutritional quality and relatively high in feed utilization rate.

The invention provides a strain for producing L-2-aminobutyric acid as well as a construction method and application of the strain. According to the strain, E.coli W3110 is taken as a chassis strain, tdh, rhtA and ilvE genes are knocked out, the transcriptional levels of ppc, aspC, thrAfbrBC, ilvAfbr, tdcB, yaS and pntAB genes are up-regulated, an ilvI gene is weakened, bcd and gdh genes derived from bacillus subtilis B.subtilis 168 and a cysK gene derived from Corynebacterium glutamicum K051 are introduced in a heterologous manner, and the L-2-aminobutyric acid producing strain is obtained through screening and screening. The strain does not contain plasmids, is stable in heredity, does not need induction, does not need to add resistant substances, efficiently synthesizes L-2-aminobutyric acid from the beginning by taking glucose as a substrate, and has good L-2-aminobutyric acid synthesis capability, stable performance and high acid production efficiency.

The invention relates to the technical field of probiotic development and utilization, in particular to a goose source Weissella cibaria F11 and application thereof. The Weissella cibarius F11 is preserved in the China General Microbiological Culture Collection Center on July 11, 2025, and the preservation number is CGMCC NO.35211. The Weissella cibarius F11 has the characteristics of good acid resistance and cholate resistance, and can be used for preparing the acid-resistant and cholate-resistant strain. And the bacillus subtilis has a good inhibition effect on pathogenic bacteria such as escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and salmonella typhimurium, and also has stable acid production capacity and good safety. The Weissella sinus F11 provided by the invention meets the probiotic characteristic standard, the antibiotic resistance spectrum of the Weissella sinus F11 meets the feed probiotic safety standard, the growth of goslings can be remarkably promoted, the death rate and diarrhea occurrence rate of the goslings can be remarkably reduced, and the weissella sinus F11 can be used for preparing feed probiotics. The development potential in the fields of preparation of bacteriostatic and antibacterial drugs, probiotics for poultry feed, feed additives, poultry feed and the like is huge.

The invention discloses a lithium battery precious metal recovery method based on microorganism-electrochemical coupling and one-way membrane separation, which comprises the following steps: step 1, pretreatment of a waste lithium battery: performing complete discharge treatment on the waste lithium battery, then disassembling the battery in an oxygen-free environment, separating out a positive electrode material and a negative electrode material, and drying the positive electrode material and the negative electrode material; electrode material black powder containing precious metal is obtained; 2, designing an anaerobic tank, and culturing and domesticating an acid-producing electroactive flora; 3, continuously and stably adding black powder into the anaerobic tank; copper electrodes are added to the two sides of the cylindrical anaerobic tank and the center of the one-way membrane, a power source is connected, the positive electrode is connected with the electrodes on the two sides of the anaerobic tank, and the negative electrode is connected with the membrane center electrode. Compared with the prior art, the method has the advantages that the solid-to-liquid ratio of microbiological leaching can be greatly increased through microbiological-electrochemical coupling and one-way membrane separation, the transfer efficiency of precious metal electrons is accelerated, anaerobic flora is protected, poison to the anaerobic flora is reduced, and the service life of equipment is prolonged.

Provided in the present application is a method for treating a heavy bis(fluorosulfonyl)imide residue, comprising the following step: causing a starting material system comprising the heavy bis(fluorosulfonyl)imide residue and an acidic aqueous solution to react to obtain a fluorine-containing acid solution and a crude sulfamic acid product, wherein the fluorine-containing acid solution can be used as a by-product acid, thereby saving treatment costs; and the crude sulfamic acid product can also be directly sold as a product, thereby reducing treatment costs, bringing economic benefits, and achieving resource recycling of the heavy bis(fluorosulfonyl)imide residue.

The invention discloses a method for enhancing release-conversion of phosphorus in anaerobic fermentation of excess sludge by humus. In order to relieve the current situation of phosphorus resource shortage and depletion at present, the invention aims to improve the residual sludge fermentation phosphorus release performance by adding humus exogenous to the anaerobic fermentation process. The method comprises the following steps: 1, pretreating the excess sludge with low-temperature heat and acid to strengthen the disintegration and lysing efficiency of extracellular polymeric substances of the excess sludge, so as to provide sufficient substrates for subsequent anaerobic fermentation acid production and improve the release efficiency of phosphorus; 2, the pretreated sludge is subjected to anaerobic fermentation, humus is added to improve the solubility of Fe-P (Fe < 3 + > is reduced into Fe < 2 + >), and the humus and metal ions are competitively complexed to improve the release efficiency of soluble phosphorus. Compared with the method for reinforcing the release-conversion of the phosphorus in the excess sludge by the humus, the method has the advantages of low cost, no secondary pollution risk and the like, can achieve an ideal phosphorus release-conversion effect, and has important significance for realizing the recovery and recycling of phosphorus resources in the sludge.

The invention belongs to the technical field of environmental engineering, and relates to a microbubble oxygen supply microorganism pretreated livestock and poultry manure fermentation acid production method, which comprises: in a microbubble oxygen supply environment, carrying out a pretreatment reaction on livestock and poultry manure and microorganisms under the action of microbubbles with a diameter of less than or equal to 100 [mu] m; the pretreated livestock and poultry manure is subjected to combined fermentation through multiple substrates, and 5.0-6.5 g / L high-concentration acid production is achieved; carrying out solid-liquid coarse separation, enabling separated acid liquid supernatant to enter a collecting tank, enabling solid-phase residues to enter a squeezer to extrude residual acid liquid, enabling squeezed liquid to flow back or be collected, and enabling the residues to be used for preparing organic fertilizer after being dehydrated. According to the process, the degradation rate of lignocellulose in the mixed substrate can be improved through pretreatment, the fermentation acid yield of the substrate is increased, and the cost and the energy consumption are both reduced; meanwhile, acid liquor and fertilizer are generated to form a double-product high-added-value utilization mode, and the environment adaptability is high.

The invention discloses a separation device for dairy cow dung water, a separation device forming method and a treatment method. The separation device comprises a desilting basin, a permeation basin, a grating, a separation layer forming area and a funnel-shaped multi-layer biomass permeation separation layer, a separation layer forming area is formed in the permeation tank under the supporting action of the supporting net, the biomass interlayer and the large-particle hard interlayer, a funnel-shaped multi-layer biomass permeation separation layer is formed in the separation layer forming area, and the funnel-shaped multi-layer biomass permeation separation layer comprises a separation layer surface layer, a separation layer bottom layer and a separation layer middle layer; the surface layer of the separation layer is used for intercepting solid matters and salt and producing acid, the bottom layer of the separation layer plays a supporting role, and the middle layer of the separation layer is used for permeating liquid dung and intercepting the solid matters. By adopting the separation device disclosed by the invention, cow dung water solids after mechanical solid-liquid separation can be efficiently intercepted on a large scale without adding exogenous substances, and salt can be effectively intercepted to reduce nitrogen element loss, so that the economic cost of dung water treatment is reduced, and nutrients are efficiently recovered.

The application provides a pediococcus acidilactici M4 for low-moisture fermented feed and an application thereof, and belongs to the field of microbial application and feed technology. The application uses the pediococcus acidilactici M4 to prepare fermented total mixed ration. The bacteria have the characteristics of high osmosis and fast acid production, and can reduce the pH value of the fermentation system in a short time; have strong broad-spectrum bacteriostatic effect on escherichia coli, chicken white dysentery salmonella and staphylococcus aureus; and perfectly adapt to the fermentation of low-moisture total mixed ration. The strain is used as the fermentation strain of low-moisture total mixed ration, can significantly reduce the content of acid detergent fiber and neutral detergent fiber, improve the crude protein level, and then improve the digestibility of the daily ration, increase the content of probiotics such as lactic acid bacteria and yeast in the daily ration, reduce the content of pathogenic bacteria such as escherichia coli in the daily ration, and also can improve the content of volatile fatty acid in the daily ration, improve the quality of the daily ration, and improve the feeding value of the daily ration.

The application relates to the field of genetic engineering, and discloses an Aspergillus niger, a construction method and application of the Aspergillus niger, and a method for producing citric acid and / or succinic acid. Aspergillus niger The Aspergillus niger is obtained by simultaneously overexpressing alpha-amylase and aspartic protease in a starting strain. The Aspergillus niger provided by the application can realize efficient degradation and conversion of starch by synergistically overexpressing amyA genes and pepA genes, solve the technical bottleneck that the original strain is limited in growth in a starch-based fermentation system and has insufficient acid production capacity, can efficiently produce succinic acid and citric acid by taking low-cost starch as the only carbon source, significantly reduce the industrial production cost of organic acid, and promote the green and sustainable upgrading of the fermentation industry.

The application discloses a production process for preparing composite carbon source by utilizing fruit and vegetable waste resources, and comprises the following steps: raw material pretreatment, anaerobic fermentation for acid production, composite enzymolysis, deep solid-liquid separation, composite blending, and stabilization treatment; the blending carbon source liquid is pumped into the stabilization treatment tank, 0.1%-0.3% of a compounded stabilizer is added, the pH value is adjusted to 6.0-7.0, normal-temperature standing is carried out for 12-24 hours, product detection and storage are carried out, the carbon source product after the stabilization treatment is detected in terms of COD, total nitrogen, total phosphorus, the pH value and the content of small-molecule organic acid, and after each index is qualified, the product is sealed and packaged and stored in a cool and dry environment, and the application solves the technical problems of high cost, poor safety, single fruit and vegetable base carbon source component, low degradation efficiency and poor product stability of traditional carbon sources.

The invention relates to a method for enhancing collaborative risk factor control in a sludge recycling process, in particular to a method for enhancing collaborative resistance gene risk control in anaerobic fermentation acid production of excess sludge through combined pretreatment of nano zero-valent iron and peracetic acid. The invention aims to solve the problem of resistance gene transfer in the sludge resource recycling process. The method comprises the following steps: naturally settling residual sludge, removing supernate to obtain concentrated sludge, and adding nano zero-valent iron and peracetic acid for combined pretreatment. And placing the treated sludge in a reaction container, aerating with nitrogen, sealing the reactor, and carrying out anaerobic fermentation in a constant-temperature oscillator. According to the invention, organic matters in the sludge can be converted into short-chain fatty acid, the structure of a resistance gene can be destroyed, and the abundance of the resistance gene in the sludge is reduced. Meanwhile, horizontal transfer of resistance genes in the sludge is inhibited, and the environmental risk is controlled. The method is applied to the field of organic solid waste resource utilization.

The invention discloses lactobacillus helveticus 5-1 separated from fermented milk and application of the lactobacillus helveticus 5-1. The lactobacillus helveticus 5-1 has the highest viable count in 12 hours at the end of the logarithmic phase; the lactobacillus helveticus 5-1 has better acid production capacity and acid resistance; the tolerance of the lactobacillus helveticus 5-1 is strong under the bile salt concentration of 0.3%; the thallus extract of the lactobacillus helveticus 5-1 has strong capability of removing DPPH (1, 1-diphenyl-2-picrylhydrazyl); the lactobacillus helveticus 5-1 is high in exopolysaccharide (EPS) production capacity. The lactobacillus helveticus 5-1 with high activity, strong oxidation resistance, excellent acid and cholate resistance and high exopolysaccharide yield is separated, screened and cultured from the traditional fermented milk in Xinjiang, so that the blank of multifunctional and high-performance autonomous strains in the prior art is filled; the application efficiency and the stability of the compound in probiotic preparations and functional foods are improved.

The invention provides a method for treating bis (fluorosulfonyl) imide heavy slag, which comprises the following steps: reacting a raw material system comprising bis (fluorosulfonyl) imide heavy slag and an acidic aqueous solution to obtain a fluorine-containing acid solution and a sulfamic acid crude product; wherein the fluorine-containing acid liquid can be used as a byproduct acid, so that the treatment cost is saved; and the sulfamic acid crude product can also be directly sold as a product, so that the treatment cost is reduced, economic benefits are brought, and resource recycling of the bis (fluorosulfonyl) imide heavy slag is realized.

The invention relates to klebsiella oxytoca and application thereof in reduction of pentavalent vanadium, and belongs to the technical field of microorganisms. The first technical problem to be solved by the invention is to provide a bacterial strain for reducing a high-concentration vanadium solution, namely Klebsiella oxytoca G-17. The Klebsiella oxytoca G-17 disclosed by the invention is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Klebsiella oxytoca G-17 is CCTCC M 20242962. The Klebsiella oxytoca G-17 has high tolerance to vanadium in the environment, the tolerance concentration to vanadium reaches up to 2000 mg.L <-1 >, the Klebsiella oxytoca G-17 can adapt to the survival condition of a high background pollution area, the Klebsiella oxytoca G-17 is reported for the first time to reduce pentavalent vanadium into tetravalent vanadium, and a bioremediation technology is provided for vanadium pollution. The vanadium reduction operation process is simple, the high reduction rate can be achieved under the condition that an electron donor does not need to be added, the reduction rate of 84.72 + / -0.17% of pentavalent vanadium can be achieved under the concentration of 500 mg / L, and the method has the advantages of being convenient to use and efficient.

The application belongs to the technical field of microorganisms, and particularly relates to a halophilic bacillus, a bacterial agent and application thereof. The application provides a halophilic bacillus Halobacillus andaensis, and the preservation number is CCTCC M 20232394. The application also provides a bacterial agent containing the halophilic bacillus. The application also provides application of the halophilic bacillus or the bacterial agent in promoting plant growth under saline-alkali stress, improving plant stress resistance, improving soil, and crop production. The halophilic bacillus provided by the application has excellent saline-alkali tolerance, and can only grow under saline-alkali conditions. Meanwhile, the halophilic bacillus has growth-promoting characteristics such as salt reduction, acid production for alkali reduction, nitrogen fixation, and the like, can reduce salt damage, and improve crop yield and quality.

The utility model relates to the technical field of fermentation, in particular to a straw micro-aerobic-anaerobic integrated biogas fermentation device. The device comprises a total fermentation box, a hot water circulation heating system and a lateral flow micro-aeration system, straw fermentation liquid is added into reaction chambers through a feeding port, high-solid hydrolysis acid production is performed in a first reaction chamber, and then anaerobic fermentation is performed in a second reaction chamber; the jacket provides heat for the reaction chamber, so that the straw fermentation liquid is fermented at a set temperature; marsh gas generated in the fermentation process is collected through a gas collecting hole; in the fermentation process, the reactor forms a good natural stirring effect through a side flow micro-aeration mechanism. The micro-aerobic-anaerobic integrated structure reduces the occupied area of the device, reduces the maintenance cost and improves the volume utilization rate.