149 results about "Volatile fatty acids" patented technology

The invention relates to the technical field of organic fertilizer preparation and waste recycling, in particular to a food industrial park wastewater recycling and sludge resourceful treatment method which comprises the following steps: step 1, dynamically partitioning wastewater and cooperatively separating grease, monitoring the conductivity and turbidity of wastewater at water outlets of all workshops in real time, and dynamically dividing wastewater load grades according to monitored values; step 2, coupling biological treatment and carbon source circulation, inputting the effluent in the step 1 into an anaerobic reactor according to a real-time COD load ratio, adding a sludge glycolysis solution as a carbon source supplement, and dynamically adjusting the adding amount based on the ratio of volatile fatty acid to alkalinity; step 3, catalytic oxidation-membrane coupling advanced treatment: carrying out catalytic ozonation on aerobic effluent through sludge-based activated carbon, and adjusting the filling amount of a catalyst in a stepped manner along with characteristic parameters of organic matters in the effluent; 4, directional glycolysis of the sludge and closed-loop utilization of products. Through monitoring and dynamic adjustment, the problems of high energy consumption, low treatment efficiency, resource waste and the like in a traditional wastewater treatment method are effectively solved.

The invention discloses a landfill leachate anaerobic-denitrification treatment process and system based on waste molasses synergistic interaction, and belongs to the technical field of sewage treatment. The process comprises the following steps: (1) carrying out two-stage anaerobic digestion on the landfill leachate to remove organic matters and recover biogas, wherein first-stage anaerobic digestion adopts medium-temperature (35 + / -2 DEG C) anaerobic digestion, and second-stage anaerobic digestion adopts high-temperature (55 + / -1 DEG C) anaerobic digestion; (2) removing impurities from the waste molasses, diluting the waste molasses, mixing the diluted waste molasses with activated sludge, fermenting under an anaerobic condition to generate fermentation liquor rich in volatile fatty acid, and adding the fermentation liquor into a denitrification reactor; and (3) constructing an anaerobic-denitrification synergistic system, and increasing the denitrification rate by using the charcoal-loaded nano zero-valent iron (nZVI at BC) as an electronic mediator. According to the method, resource utilization of the waste molasses is realized, the problem of imbalance of the carbon nitrogen ratio of the landfill leachate is solved, anaerobic digestion and nitrification and denitrification processes are coupled, efficient nitrogen removal and organic matter removal are realized, and meanwhile, the sludge yield and the operation cost are reduced.

The invention belongs to the technical field of biological feed, and particularly relates to a composite silage leavening agent, silage and application. The compound silage leavening agent is prepared from the following components: lactobacillus plantarum and vitamin enzyme, wherein the concentration of the lactobacillus plantarum is greater than or equal to 1 * 10 < 9 > CFU / kg, and the concentration of the cellulase is 1 * 10 < 6 >-1 * 10 < 8 > U / kg. In the preparation process of the silage, due to the synergistic effect of cellulase and lactobacillus plantarum, escape of nutrient substances such as ammoniacal nitrogen is reduced, the content of nutrient substances such as polypeptide is increased, and the nutritional value of protein of the silage is reserved. The ensiling feed disclosed by the invention is good in fermentation quality and rich in nutrient substances, and can improve the digestibility and the total volatile fatty acid yield of mutton sheep when being used for feeding the mutton sheep; and meanwhile, efficient conversion of silkworm branch and leaf byproducts can also be realized.

The invention provides a microbial agent for producing acid through fermentation of organic waste and application of the microbial agent, and relates to the technical field of biology, the microbial agent contains Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus, and the microbial agent is prepared from the following raw materials: Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus. Studies find that acetobacter burgii, clostridium cow dung and eubacterium mucilaginosus complement each other in the anaerobic fermentation process, the degradation efficiency of organic matters such as carbohydrates and proteins in the organic wastewater / perishable garbage is improved, the yield of volatile fatty acids is remarkably increased, meanwhile, the emission of CO2 is reduced, and the environmental pollution is reduced. The method has the application prospects of organic wastewater / perishable garbage treatment and recycling. When the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the fermented mixed acid liquid is rich in VFA and can be used as an organic carbon source, and the additional value is higher; when the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the operation is simple and convenient, the cost is low, biogas slurry treatment links and cost are reduced, the added value of products can be improved, and the acid-producing bacterium agent has an engineering application prospect.

The invention provides a method for increasing anaerobic digestion productivity of waste and relieving acid inhibition by adopting nano bubble water. The method comprises the following steps: preparing hydrogen-nano bubble water, preparing inoculation mud, mixing the hydrogen-nano bubble water, the inoculation mud and agricultural wastes, and carrying out anaerobic digestion treatment; according to the method, in the anaerobic digestion process, the hydrolysis rate can be increased, the hysteresis period can be shortened, the methane yield can be increased, ammonia / acid inhibition can be effectively relieved, the consumption speed of volatile fatty acid is increased by 35% or above, and trans-fatty acid inhibition can be effectively relieved. And an innovative solution thought is provided for relieving the inhibition phenomenon generally occurring in the AD process.

The invention discloses a method for preparing a denitrification composite carbon source by using kitchen waste, and relates to the technical field of kitchen waste resourceful treatment, and the method comprises the following specific steps: pretreatment, oil removal, hydrolytic acidification, solid-liquid separation, biochar preparation and carbon source compounding. Wherein the yield of volatile fatty acid (VFAs) can be remarkably improved by performing damp-heat pretreatment on fermentation, the biochar provides an adsorption carrier and a microorganism attachment site for the VFAs, the release period of a carbon source is prolonged, and a carbon source-carrier-catalyst ternary system is formed by the biochar, the VFAs and divalent metal cations, so that the denitrification rate is improved. The cost of the denitrification composite carbon source is obviously lower than that of a traditional commercial carbon source, and deep resource utilization of the kitchen waste is achieved.

The invention discloses a schizochytrium limacinum strain for efficiently converting kitchen waste to produce single-cell protein and application of the schizochytrium limacinum strain, and belongs to the technical field of biological engineering. The preservation number of the schizochytrium sp LOX-2 is CGMCC (China General Microbiological Culture Collection Center) No. 41623. The invention also discloses a method for producing single-cell protein by efficiently converting kitchen waste. The method comprises the following steps: fermenting food waste to produce volatile fatty acid; and culturing schizochytrium limacinum by using the strain as a carbon source to obtain single-cell protein. According to the recombinant strain spLOX-2, the utilization of lactic acid is improved, the biomass and the protein yield of the recombinant strain spLOX-2 are respectively improved by 14.19% and 12% compared with those of a wild strain, and the grease content is obviously improved by 107.1%. According to the invention, a new way is provided for a sustainable biological economic mode, wastes are converted into valuable biological chemicals, and a solid foundation is laid for expanding the scale of the process and the economic feasibility of the process in industrial application.

The invention discloses a system for producing short-chain volatile fatty acid through kitchen co-fermentation of sludge, and relates to the technical field of biochemical engineering and environmental engineering. Comprising a time phase construction module, a causal playback identification module, a flow field mapping correction module, a metabolism gating feedback module, a structure reconstruction steady-state module and a phase traction control module, and reconstructing a gas-liquid phase difference reference surface according to the real-time flow field response, and establishing a reference surface to calibrate an anti-phase trigger window. According to the method, gas-liquid reverse-phase accurate identification is realized through a high-precision time base line and flow field tracking, a causal chain is constructed to early warn an inverted nucleation area in advance, a steady-state energy base plane is established in combination with three-dimensional flow field correction and metabolism monitoring feedback and driving structure cooperative adjustment, and finally disturbance suppression and path self-resetting are realized through phase traction regulation. The acid production efficiency and the system stability are improved.

The invention discloses a method and a device for monitoring the stability of an anaerobic digestion system in real time. The method comprises the following steps: 1, carrying out non-targeted metabonomics analysis on the anaerobic digestion mVOCs category by adopting headspace solid-phase microextraction in combination with gas chromatography-high resolution mass spectrometry; 2, screening differential mVOCs markers based on principal component analysis and orthogonal partial least squares discriminant analysis; and 3, constructing a system stability real-time monitoring system by using an electronic nose sensor array, and when the difference mVOCs marker concentration deviates from a threshold value or difference categories are continuously accumulated, triggering early warning and recommending an optimization strategy by the system. The method and the device can monitor the state of the anaerobic reactor in real time, early warn the methane production inhibition risk in advance compared with traditional parameters (pH, volatile fatty acid and the like), improve the biogas yield and reduce the operation and maintenance cost, are suitable for intelligent management of anaerobic digestion scenes of kitchen garbage, municipal sludge, agricultural waste and the like, and can also be expanded to other anaerobic biological fermentation fields.

A metrological device (10) configured to generate at least one energetic / electrochemical parameter from measurement data based on bioelectrochemical processes, comprising at least one metrological microbial electrochemical technology unit (11) with a cathodic region (11.1) sealed off from the fluid to be examined, and comprising a logic unit (16) with at least one microcontroller (16.1) configured to process measurement data collected over an evaluation period, wherein the metrological device (10) is configured to correlate at least one measured value with at least one process parameter (P SB ) based on the processed measurement data, in particular with a process parameter (P SB) for the chemical / biological oxygen demand (COD / BOD) and / or for a carbon consumption rate and / or for substance fractions of volatile fatty acids; characterized in that the measuring device (10) has at least one active ceramic (12.2) arranged between the cathodic region (11.1) and the fluid to be examined as a component of the metrological microbial electrochemical technology unit (11), which separates the cathodic region (11.1) from the fluid to be examined, wherein the measuring device (10) is configured by means of the at least one ceramic (12.2) to generate the at least one energetic / electrochemical parameter from measurement data collected over an evaluation period of at least 30 days, in particular measurement data from cell-internal resistance components and / or voltage, capacitance, current, power, and / or electrical charge measurement data.

The invention discloses a copper-nickel alloy nanoparticle catalyst and a preparation method and application thereof in microbial electrosynthesis, and belongs to the technical field of energy conversion and microbial electrosynthesis, the preparation method comprises the following steps: dissolving Cu (NO3) 2 and Ni (NO3) 2 in a mixed solution of water and ethanol, dispensing the mixed solution on a carbon-based material, drying the surface by baking and drying overnight, and obtaining the copper-nickel alloy nanoparticle catalyst. A copper-nickel loaded carbon-based material is obtained; calcining the copper-nickel loaded carbon-based material in air at 350-450 DEG C to obtain a copper-nickel oxide loaded carbon-based material; and reducing the carbon-based material loaded with the copper-nickel oxide in flowing H2 at 500-600 DEG C to obtain the copper-nickel alloy nanoparticle catalyst. According to the method, the volatile fatty acid can be synthesized by taking CO2 as a raw material, and the product yield and the Faraday efficiency are relatively high. The method is simple to operate, mild in condition and low in cost, and has relatively high environmental benefits.

The invention relates to the technical field of feed production, in particular to an azolla filliculoidas fresh leaf fermented feed which comprises the following components in parts by weight: 70 parts of azolla filliculoidas silage sections, 20 parts of a composite carrier matrix, 5 parts of a natural anti-caking and toxin adsorbent, 7 parts of functional liquid, 10 parts of an energy and flavor synergist and 2 parts of zymophyte. Functional liquid is converted into chitosan oligosaccharide through ultrasonic treatment, and long-chain chitosan is broken into high-solubility chitosan oligosaccharide; through cold plasma activation, an active functional group is grafted on the surface of a chitosan oligosaccharide molecule to form a nanoscale compact protective film; the protective film can inhibit pollution of aerobic mould and saccharomycetes after unsealing, prolong the shelf life of the feed, reduce loss of volatile fatty acids and flavor substances generated by fermentation, and maintain the nutritional value and palatability of the feed.

The application provides a pediococcus acidilactici M4 for low-moisture fermented feed and an application thereof, and belongs to the field of microbial application and feed technology. The application uses the pediococcus acidilactici M4 to prepare fermented total mixed ration. The bacteria have the characteristics of high osmosis and fast acid production, and can reduce the pH value of the fermentation system in a short time; have strong broad-spectrum bacteriostatic effect on escherichia coli, chicken white dysentery salmonella and staphylococcus aureus; and perfectly adapt to the fermentation of low-moisture total mixed ration. The strain is used as the fermentation strain of low-moisture total mixed ration, can significantly reduce the content of acid detergent fiber and neutral detergent fiber, improve the crude protein level, and then improve the digestibility of the daily ration, increase the content of probiotics such as lactic acid bacteria and yeast in the daily ration, reduce the content of pathogenic bacteria such as escherichia coli in the daily ration, and also can improve the content of volatile fatty acid in the daily ration, improve the quality of the daily ration, and improve the feeding value of the daily ration.

The present invention discloses a Schizochytrium strain that efficiently converts food waste to produce single-cell protein and its application, belonging to the field of bioengineering technology. The Schizochytrium spLOX‑2 deposit number is: CGMCC No.41623. The present invention also discloses a method for efficiently converting food waste to produce single-cell protein, fermenting food waste to produce volatile fatty acids; and using it as a carbon source to culture Schizochytrium to obtain single-cell protein. The recombinant strain spLOX‑2 in the present invention has improved utilization of lactic acid, and its biomass and protein yields are increased by 14.19% and 12% respectively compared with the wild-type strain, and the oil content is significantly increased by 107.1%. The present invention provides a new path for a sustainable bioeconomic model, in which waste is converted into valuable biochemicals, laying a solid foundation for expanding the scale of the process and the economic feasibility in industrial applications.

The application belongs to the field of environmental microbiology, and particularly relates to a Comamonas testosteroni G314 and application thereof.The technical points are as follows: the Comamonas testosteroni provided by the application is preserved in the China General Microbiological Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, No. 1, Xibahe Road, Chaoyang District, Beijing, China, and the preservation number is CGMCC No. 32129, and the preservation time is September 30, 2024.The Comamonas testosteroni provided by the application has different abilities to synthesize PHA by using different organic acid carbon sources, and the type of PHA can be regulated by regulating the composition of volatile fatty acids, so that the performance of PHA is improved, new strains for efficient production of PHA are provided, and the production cost of PHA is significantly reduced.

The invention provides an on-line volatile fatty acid monitoring system for water quality, which can effectively eliminate or reduce the influence of main interferents such as ammonia nitrogen to the greatest extent, realizes high-precision, high-selectivity and full-automatic on-line continuous monitoring, and has good economical efficiency and stability. The system comprises: a sampling and pretreatment unit, which is used for extracting a water sample and removing solid particles and suspended solids in the water sample through an on-line filtering device; the ammonia nitrogen separation unit is used for carrying out first distillation stripping operation on the pretreated water sample; the VFA separation unit is used for acidizing the water sample from which ammonia nitrogen is removed, and then carrying out secondary distillation stripping operation in an accurately controlled acidic environment; the VFA quantifying unit is used for absorbing VFA steam separated from the secondary distillation process by using quantitative set absorption liquid, measuring the conductivity change and real-time temperature of the absorption liquid before and after absorption through a sensor, and calculating the VFA concentration based on the change; and a control and data processing unit.

This application relates to the field of waste treatment technology, and particularly to a method for treating kitchen waste, a carbon source, and its application. The kitchen waste treatment method includes the following steps: directional fermentation of kitchen waste in an acidic environment to obtain a fermentation mixture; filtration of the fermentation mixture to obtain a filtrate; and sequential distillation and condensation of the filtrate to collect volatile organic compounds, resulting in a condensate containing volatile fatty acids and low-molecular-weight alcohols. The method in this application involves directional fermentation, resulting in a fermentation mixture rich in volatile organic compounds, including volatile fatty acids (VFAs) such as propionic acid, butyric acid, and acetic acid, and low-molecular-weight alcohols such as propanol and ethanol. Filtration further reduces the nitrogen content and suspended solids value of the filtrate. Distillation causes these small-molecule organic compounds in the filtrate to evaporate, while water evaporates minimally, and the nitrogen content in the filtrate hardly evaporates. The resulting condensate has a high COD value, a high content of small-molecule organic compounds, and an extremely low nitrogen content.

The invention discloses a binary pseudocapacitance composite material for promoting anaerobic digestion of kitchen waste and a preparation method and application thereof, and belongs to the technical field of organic solid waste recycling and renewable energy sources. The wood chip biochar-polydopamine-Fe3O4 composite material with a core-shell structure is constructed by taking wood chips as raw materials, preparing biochar through low-temperature pyrolysis, forming an interface modification layer through polydopamine coating, loading Fe < 3 + > and carrying out secondary pyrolysis to generate Fe3O4 nanoparticles (40-110nm). The material can be recovered through a magnetic field (the recovery rate is greater than or equal to 90%), when the dosage is 0.8-1.2 g / L, the peak value of volatile fatty acid in an anaerobic digestion system is obviously reduced to be less than or equal to 400 mg / L, the methane accumulation yield is improved to be greater than or equal to 45%, the electron transfer efficiency of acid-producing bacteria and methanogens is synchronously optimized, and the system operation is stabilized.

The invention belongs to the technical field of organic waste treatment, and particularly relates to a multi-parameter regulation and control high-cellulose organic waste anaerobic digestion treatment method. According to the method provided by the invention, high-cellulose wastes such as traditional Chinese medicine residues, olive residues and vinasse are subjected to alkali liquor, hydrothermal or biogas slurry pretreatment, the contents of reducing sugar, volatile fatty acid (mainly acetic acid) and ammonia nitrogen are synchronously monitored, and the pretreatment conditions are dynamically adjusted according to preset thresholds (the reducing sugar is less than or equal to 50mg / g, the acetic acid is obviously higher than propionic acid, and the ammonia nitrogen is less than or equal to 7mg / g). The method disclosed by the invention realizes cooperative regulation and control of hydrolysis promotion and digestion stability, effectively avoids acidification and ammonia inhibition, improves the methane yield and the system operation stability, and is suitable for efficient anaerobic digestion treatment of various high-cellulose organic wastes.

The present invention provides a method for predicting the tendency of weight loss in Equus animals by evaluating multiple markers in a sample obtained from an Equus animal. Such markers include fermentation products and / or their metabolites, such as volatile fatty acids, bacterial population diversity indicators, and the abundance / relative abundance of specific bacterial taxa.

The invention provides a method for highly converting algae organic solid waste into a biological carbon source. According to the method disclosed by the invention, the algae organic solid waste is directly treated by using an electrochemical means under the condition of not additionally adding an agent, so that the biodegradability of the algae organic solid waste is improved, the efficient resource utilization of the algae organic solid waste is further improved, the cost is reduced, and the risk of generating by-products due to additionally adding the agent is reduced; the electrochemical means has the characteristics of high efficiency, good universality, high cleanliness, environmental friendliness and the like, can remove algae in the water body without generating toxic and harmful by-products, can ensure the safety of the water body and the natural ecological safety of the subsequent water body, and also avoids the use of other additional medicaments; the yield of the volatile fatty acid generated in the fermentation process of the pretreated substrate is remarkably increased, reaches 5541 mg / L to the maximum, and is increased by 19% compared with the volatile fatty acid generated after enrichment of single algae.

The invention discloses an intelligent adjusting tank system for carbon source recovery, and relates to the technical field of sewage treatment, the intelligent adjusting tank system comprises an intelligent homogeneous adjusting tank, the intelligent homogeneous adjusting tank comprises an adjusting tank body, and the inner side of the middle part of the adjusting tank body is connected with the bottom end of a diversion annular sleeve; the device further comprises an adjusting tank water distribution mechanism, a homogeneous stirring mechanism, a hydrolytic acidification-membrane separation coupling tank and an intelligent dosing unit, wherein the adjusting tank water distribution mechanism is mounted in the middle of the arc-shaped tank bottom; the homogeneous stirring mechanism is mounted in the middle of the regulating tank body; according to the intelligent adjusting tank system for recycling the carbon source, the quality of inflow water is monitored and pre-adjusted in real time through the intelligent homogeneous adjusting tank, the buffering capacity on inflow water impact load is high, it is ensured that wastewater entering the hydrolytic acidification cavity is in the optimal reaction condition, volatile fatty acid is enriched and recycled through the membrane separation effect, and the yield is high. The recovered carbon source can be directly used for a subsequent biological nitrogen and phosphorus removal process, so that the problem of insufficient carbon source during nitrogen and phosphorus removal of industrial wastewater with low carbon nitrogen ratio is effectively solved.

The present invention discloses a method for promoting the anaerobic digestion process of sludge by coupling treatment of oxygen and corn straw biogas residue biochar, belonging to the technical field of solid waste resource treatment. The present invention discloses a method for promoting the anaerobic digestion process of sludge by coupling treatment of oxygen and biogas residue biochar. The method increases the anaerobic hydrolysis and acidification rate of sludge by injecting a trace amount of oxygen, and at the same time utilizes the oxygen-containing functional groups on the surface of biogas residue biochar as a bridge to accelerate the electron transfer between volatile fatty acid oxidizing bacteria and methanogenic archaea, promote the degradation of volatile fatty acids, increase the maximum methane production potential, promote the maximum methane production rate and increase the cumulative methane production, which is conducive to further improving the economic and environmental benefits of anaerobic digestion technology.

The invention discloses a method for producing volatile fatty acid by improving microbial electro-catalytic conversion of CO2. The core of the method is to construct an efficient and stable heterozygote biological cathode. The method comprises the following steps: inoculating flora mainly comprising Acetobacter burgii and Desulfurization Vibrio in a cathode chamber of an H-type microbial electrosynthesis reactor, and adding a zinc source and a sulfur source to synthesize ZnS nanoparticles in situ on the surfaces of cells, so as to construct the bacterium and ZnS nanoparticle heterozygote biological cathode. The acetic acid yield of the heterozygote biological cathode can reach 3.38 mmol / L / d, and the accumulative yield of one batch is 20.25 mmol / L, which is 2.7 times that of an unmodified carbon cloth biological cathode; the coulombic efficiency of the total volatile fatty acid product reaches 96.47% and is 1.25 times that of a carbon cloth biological cathode; and the coulombic efficiency of acetic acid and the selectivity of acetic acid are respectively improved to 83.27% and 86.32%, which are obviously higher than 40.63% and 52.64% of those of a carbon cloth biological cathode. In addition, the charge transfer resistance of the heterozygote biological cathode is as low as 3.75 ohm and is obviously superior to that of a carbon cloth biological cathode (25.28 ohm), so that long-term stable and high-selectivity acetic acid production is realized.

The invention relates to a high-efficiency nitrogen removal method based on coupling of anaerobic hydrogen production and hydrogen type autotrophic denitrification, which comprises the following steps: S1, using high-concentration organic wastewater with the COD (Chemical Oxygen Demand) concentration of more than or equal to 5000mg / L, carrying out mechanical crushing and homogenization treatment to ensure that the particle size of particles is less than or equal to 1mm, and carrying out ultrasonic treatment to enhance the dissolution of organic matters; s2, firstly inoculating acid-producing bacteria into the wastewater, and carrying out continuous stirring fermentation at 34-38 DEG C, so that the acid-producing bacteria convert complex organic matters into volatile fatty acid (VFA), and the concentration of the VFA reaches 2000-3000mg / L; adjusting the pH value to 5.5-6.5, inoculating hydrogenogens, and fermenting to produce hydrogen; s3, absorbing tail gas generated in the fermentation process with alkali liquor to obtain purified hydrogen, and collecting the purified hydrogen; and S4, loading a filler in an up-flow packed bed reactor, inoculating hydrogen autotrophic denitrifying bacteria, injecting hydrogen into the reactor through a hollow fiber membrane gas distribution device, enabling the bubble diameter to be less than or equal to 50 microns, controlling the water temperature to be 30-35 DEG C, and controlling the HRT to be 4-12 hours. Hydrogen is obtained through fermentation of organic waste and used for a denitrification system, the hydrogen utilization rate is increased, energy consumption is reduced, and the green circulation target of treating waste with waste is achieved.

The invention discloses a method for promoting rapid recovery of a mixed fermentation system based on a complex microbial inoculant and cow dung. The method comprises the following steps: firstly, treating a mixed material of chicken manure and straw pulping waste liquid; then, when the methane production rate of the mixed fermentation system is reduced by more than 10% or the hydrogen sulfide content is higher than 100ppm, starting a recovery program; the method comprises the following steps: firstly, mixing a complex microbial inoculant into feed fed into an integrated device, then, feeding pretreated cow dung into a fermentation methane production area of the integrated device, and continuously monitoring the gas production rate, methane content, hydrogen sulfide content and volatile fatty acid concentration of a system. Meanwhile, pretreated cow dung is added into a fermentation methane production area of the integrated device so as to supplement methane bacteria, and two-phase anaerobic process parameter optimization is combined, so that rapid and stable recovery of the system is realized.

The invention discloses a method for household preparation of a solid-state incentive element slow-release fertilizer from kitchen garbage, and belongs to the technical field of organic solid waste resourceful treatment. According to the invention, the kitchen garbage is crushed and pulpified; then decomposing organic matters in the kitchen garbage into plant growth excitant substances such as monosaccharide, volatile fatty acid and the like by utilizing composite biological hydrolase; then a chemical catalyst is added to promote generation of humic acid, rapidly available phosphorus, rapidly available potassium and other plant growth excitants, and meanwhile, residual solid lignin and cellulose form a porous hydrothermal carbon material. And finally, performing dehydration and drying treatment to promote the precipitation of an excitant substance, and stably adsorbing the excitant substance on the hydrothermal carbon material to form the solid-state excitant slow-release fertilizer. The method disclosed by the invention can be carried out in a normal-pressure container, does not add corrosive, toxic or odorous substances, and is completely suitable for being carried out in a family environment. The plant fertilizer efficiency of the prepared solid-state incentive element slow-release fertilizer is equivalent to that of a chemical compound fertilizer, preparation can be completed within 8 hours, the solid-state incentive element slow-release fertilizer is completely different from a traditional biological composting process and is also different from a preparation process of charcoal or hydrothermal fertilizer, and the solid-state incentive element slow-release fertilizer has obvious creative significance.

This invention relates to the field of waste sludge treatment technology, specifically to an in-situ resource utilization method for a microbial-algae system and its application. The in-situ resource utilization method for the microbial-algae system includes: placing waste sludge, microalgae, and sodium sulfite in an anaerobic fermentation reactor, then adjusting the pH of the mixture and fermenting under anaerobic conditions; wherein the microalgae include at least one of the phyla of green algae, cyanobacteria, and diatoms; and no anaerobic sludge is added to the anaerobic fermentation reactor. This method can effectively utilize the microbial-algae system to increase the yield of volatile fatty acids during anaerobic fermentation in situ, and can also reduce the yield of waste sludge in situ, thereby lowering the subsequent disposal costs of sludge and microalgae in the secondary sedimentation tank.

The invention provides a compound bacteria preparation for sludge treatment and a preparation method of the compound bacteria preparation. The preparation method comprises the following steps: S1, preparing stearamide propyl phosphate betaine-PVA microgel; s2, preparing stearamide propyl phosphate betaine-PVA (Polyvinyl Alcohol) microgel / SiO2 composite microspheres; s3, carrying out high-temperature carbonization on the stearamide propyl phosphate betaine-PVA microgel / SiO2 composite microspheres under the protection of inert gas, so as to obtain C / SiO2 composite microspheres; and S4, adding the C / SiO2 composite microspheres into the mixed bacterial liquid, introducing nitrogen, heating and stirring to obtain the composite bacterial preparation for sludge treatment. According to the method, sphybrid conjugate electrons of a porous carbon matrix are prepared, distribution of electron clouds of refractory organics in the sludge is adjusted through interface action, active sites are exposed, degradation activation energy is reduced, decomposition of the refractory organics into volatile fatty acid is accelerated, and microspheres of a porous structure can also reduce mass transfer obstruction caused by thallus aggregation, so that the degradation efficiency of the sludge is improved. The sludge treatment is facilitated.

The invention discloses an application of alpha-ketoglutaric acid in improving ruminant rumen fermentation and promoting microbial protein synthesis. The invention discloses application of alpha-ketoglutaric acid in preparation of a feed additive for improving ruminant rumen fermentation and promoting microbial protein synthesis. The invention discloses an application of alpha-ketoglutaric acid in preparation of a feed for improving ruminant rumen fermentation and promoting microbial protein synthesis. The alpha-ketoglutaric acid can increase the concentration of total volatile fatty acids (including acetic acid, propionic acid and butyric acid), reduce the concentration of ammonia nitrogen in rumen, increase the concentration of microbial protein, improve the feed efficiency, promote the synthesis of the microbial protein and improve rumen fermentation.