23 results about "Glycogen" patented technology

The present invention relates to a process for purifying phycocyanins from a solution comprising phycocyanins and glycogen, said phycocyanins being produced by microorganisms belonging to the order Cyanidiales, characterized in that it comprises (i) an enzymatic degradation step of glycogen with an enzyme suitable for degrading glycogen at a pH below 6 and a reaction temperature below 40°C, said enzyme being selected from a pectinase, a glucoamylase, or a mixture of a pectinase and a pullulanase, and (ii) a step of separating phycocyanins from the glycogen degradation products.

This invention belongs to the field of heavy metal removal technology, specifically disclosing an extract from the leaves of a Solanaceae plant, its extraction method, and its application. The extraction method includes the following steps: S1, mixing tomato and pepper leaves, chopping them to obtain a preliminary mixture; S2, mixing a compound microbial agent with the preliminary mixture obtained in S1, fermenting, sterilizing, centrifuging, filtering through a 0.45μm filter membrane, discarding the precipitate, and obtaining a fermented preliminary mixture; S3, concentrating the fermented preliminary mixture obtained in S2 under reduced pressure to obtain a concentrated fermentation broth; S4, adding sodium carboxymethyl cellulose to the concentrated fermentation broth obtained in S3, stirring evenly, and freeze-drying to obtain the Solanaceae plant leaf extract. This Solanaceae plant leaf extract can effectively remove cadmium from aquatic products, aiding in the growth recovery of aquatic products after cadmium stress, and can also effectively maintain the pH value of white clam muscle, reducing lactic acid accumulation and glycogen consumption, and maintaining the freshness of the meat.

Provided herein is a noninvasive method for measuring pH, glucose and glycogen levels using a single acquisition method, as well as systems for performing the method. This method for glycogen / glucose (and pH) detection has high specificity enabled by superior chemical shift separation in high-strength magnetic fields. The methods have applications in detecting changing tumor microenvironments in cancerous cells for measuring important metrics for noninvasive and quantitative metabolic profiling.

The present application relates to the technical field of biotechnology, and particularly relates to a tagatose-6-phosphate phosphatase mutant and a method for synthesizing tagatose from scratch. The method provided by the present application is a one-pot method for biosynthesizing D-tagatose by using starch or dextrin as a substrate, adopting a multi-enzyme cascade reaction system composed of a tagatose-6-phosphate phosphatase mutant, a glycogen-debranching enzyme, an alpha-glucan phosphorylase, a phosphoglucomutase, a glucose-6-phosphate isomerase, a D-tagatose-6-phosphate-3-epimerase and a 4-alpha-glucan transferase. The method can prepare tagatose from ordinary and inexpensive starting materials (starch or dextrin) at a high yield, improves the overall conversion rate of the system and the final yield of tagatose, provides a new technical approach for realizing low-cost and green production of tagatose, and has the advantages of high yield, low cost and being more suitable for industrial production.

This disclosure relates to the use of glycogen phosphatase B agonists in the treatment of neurodegenerative diseases. Specifically, this disclosure provides the use of glycogen phosphatase B agonists (preferably adenosine monophosphate) in the preparation of medicaments for the prevention or treatment of neurodegenerative diseases. This disclosure demonstrates that glycogen phosphatase B agonists (AMPs) hold promise as a safe and effective treatment option for patients with neurodegenerative diseases.

The application of ceramide in the control of rice blast fungus falls within the field of biological control. This invention relates to the application of ceramide in the control of rice blast fungus: a combination of ceramide and lipopeptides is used for the control of rice blast fungus. Exogenous addition of ceramide significantly enhances the stress effect of lipopeptides on rice blast fungus. It also enhances the abnormal accumulation of glycogen and lipid droplets in conidia, promotes the bursting of ROS, and increases intracellular ceramide and calcium... 2+ The invention increases the content of malondialdehyde (MDA) in mycelium, enhances membrane lipid peroxidation, and decreases ergosterol content. Furthermore, it inhibits the expression of ergosterol synthesis genes, UPR target genes, and sphingolipid metabolism-related genes. This invention develops a highly efficient natural agent against rice blast by combining exogenous Cer with lipopeptides. This agent effectively reduces the pathogenicity of rice blast fungus and significantly inhibits its infection of rice, laying the foundation for the development of novel biological control agents.

A recombinant adeno-associated virus (rAAV) useful for treating type II glycogen storage disease (Pompe) disease is provided. The rAAV comprises an AAV capsid which targets cells of at least one of muscle, heart, kidney, and the central nervous system and which has packaged therein a vector genome comprising a nucleic acid sequence encoding a human acid-α-glucosidase hGAA780I protein or fusion protein under the control of regulatory sequences which direct its expression. Also provided are methods of making and using this rAAV.

This invention relates to a pullulanase mutant with improved glycogen debranching activity, its preparation method, and its applications, belonging to the fields of enzyme engineering and biotechnology. The mutant is obtained by mutating tryptophan at position 81 of the type I pullulanase shown in SEQ ID NO.1 to alanine or tyrosine, with amino acid sequences shown in SEQ ID NO.2 and SEQ ID NO.3, respectively. Enzymatic performance tests show that this mutant significantly enhances the hydrolytic ability of glycogen while maintaining its original catalytic activity, providing a tool for the controlled synthesis and modification of glycogen. This invention also provides the gene encoding the mutant, a recombinant vector containing the gene, an engineered bacterium, and the application of the mutant in glycogen debranching. The pullulanase mutant provided by this invention can be effectively used for glycogen debranching treatment, expanding the application scope of type I pullulanase in glycogen-related fields.

The invention relates to the field of food processing, in particular to a preparation method of whole-plant fried ginseng and application of the whole-plant fried ginseng in preparation of drugs or food for resisting oxidation and relieving fatigue. The method comprises the following steps: fresh ginseng is taken and cleaned, rhizome, main roots and fibrous roots are separated, the main roots are cut into slices or segments, the rhizome, the slice-shaped main roots or the segment-shaped main roots and the fibrous roots are respectively placed in oil to be fried, and when the rhizome, the slice-shaped main roots or the segment-shaped main roots and the fibrous roots are golden yellow, the rhizome, the main roots and the fibrous roots are fished out, drained off oil and cooled. The antioxidant activity of the fried ginseng slices and segments is improved. Compared with fresh ginseng, the deep-fried ginseng slices and segments can significantly prolong exhaustive swimming time of load-bearing mice, significantly improve liver / muscle glycogen, lactic dehydrogenase content and superoxide dismutase activity, reduce lactic acid, urea nitrogen and malondialdehyde content, and have a significant anti-fatigue effect. According to the method, dryness of fresh ginseng can be removed, the eating fragrance can be improved, the fried ginseng product is instant and convenient to eat, and the fried ginseng product is matched with barbecue kebabs and stewed together with livestock and poultry meat, so that the fried ginseng product is delicious and health-care.

The present application discloses a rare ginsenoside composition and its application in anti-fatigue and liver protection, and relates to the technical field of biological medicine. The present application provides a rare ginsenoside composition, which is composed of Rh4, F4 and Dehydroprotopanaxatrial II, and the mass ratio of Rh4, F4 and Dehydroprotopanaxatrial II is (2-4):(2-4):1. The rare ginsenoside composition has the effect of significantly relieving physical fatigue, can reduce the decomposition and metabolism of protein to energy, eliminate fatigue, increase the liver glycogen reserve in the body, improve the fatigue resistance, has the effect of liver protection, enhances the antioxidant defense ability of liver tissue, reduces the lipid peroxidation damage, and improves the lipid metabolism disorder of liver tissue.

This invention relates to the field of biotechnology, and more particularly to a method for alleviating high-starch-induced liver damage and glucose metabolism disorders in sea bass. The feed formulation proposed in this invention comprises 20%-22% cassava starch and 2%-6% mulberry leaf polysaccharides, among other components. This application discloses that mulberry leaf polysaccharides and the high starch content in the feed form a highly efficient synergistic effect, significantly improving the growth performance of sea bass, effectively reducing the accumulation of glycogen and fat in the liver, and significantly improving liver health. Simultaneously, it optimizes the blood glucose regulation capacity of sea bass, alleviates insulin resistance caused by a high-starch diet, and enhances insulin sensitivity. Furthermore, by activating mechanisms such as the PI3K / Akt signaling pathway, it optimizes the glucose metabolism pathway and promotes efficient energy utilization. The feed formulation of this invention is economical and efficient, providing solid scientific support for the healthy aquaculture of sea bass and powerfully promoting the sustainable development of aquaculture.

A composition is disclosed for use in restoring and / or maintaining a healthy genital ecosystem. The composition comprises i) a postbiotic, ii) a plant-based glycogen and iii) plant-based stem cells.

The present disclosure relates to a waste valorization and bioresource recovery system and process for bioresource recovery from a mixed feedstock. Disclosed also are processes for producing a biopolymer (e.g., EPS, PHA, glycogen, chitin, chitosan), processes for recovering PHA from homogenized microbial biomass, processes for producing hydrocolloids and glycogen, processes for producing thin film biomaterials and rigid micro-carriers, and biodegradable and compostable biomaterial products.

The present application relates to the field of feed, especially to a feed additive for improving meat quality of ruminants and a preparation method thereof; the present application forms functional complementation through a traditional Chinese medicine composition and guanidoacetic acid and magnesium ions; acanthopanax-scutellaria promotes body synthesis metabolism, and is synergistic with creatine precursors provided by guanidoacetic acid to optimize muscle energy reserves; schisandra chinensis and rhodiola enhance the body's stress resistance, and cooperate with magnesium ions to stabilize neuromuscular excitability and reduce excessive consumption of glycogen caused by pre-slaughter stress; polygonatum and polygonatum maintain cell membrane integrity in cooperation with vitamin E and the like; through the combined action of various components through multiple targets and multiple pathways, the synchronous improvement of muscle development and stress resistance is realized.

Disclosed are pharmaceutical compositions formulated from immunomodulators formed from glycogen nanoparticles, such as monodisperse phytoglycogen nanoparticles, complexed with a pattern recognition receptor agonist, such as a TLR agonist which can be poly I:C. Also disclosed are methods of treating solid tumor cancers using the described immunomodulators, and uses of the immunomodulators to treat solid tumor cancers and to manufacture medicaments to treat solid tumor cancers.

Use of a ketone body and a ketone body ester for preserving glycogen and / or protein, thereby reducing muscle breakdown and improving endurance during exercise and promoting muscle recovery following exercise. Also for retarding muscle wasting in aging and disease. (R)-3-hydroxybutyl (R)-3-hydroxybutyrate for use in reducing muscle glycogen and protein breakdown during exercise or decreasing muscle wasting in aging and disease. Certain esters of hydroxybutyrate monomers, particularly a monoester of D-β-hydroxybutyrate with R-1,3-butanediol or with glycerol to reduce muscle breakdown and aid muscle recovery after exercise or retard muscle wasting. Compositions containing the ketone bodies or ketone body esters are also described.

The present application relates to a composition with skin smoothing and tightening function, a skin care product and a preparation method. The composition with skin smoothing and tightening function comprises a yeast / rice fermentation product filtrate, a hydroxypropyl tetrahydropyran triol and glycogen. The composition has good skin barrier repair effect, can significantly improve the skin roughness and elasticity weakened skin problems, and improve the skin smoothness and tightness. In addition, the composition has clear effective components and simple composition, and the product production process is easy to control and can be mass produced.

The present application relates to sewage treatment technical field, especially a kind of continuous flow aerobic granular sludge culture device and method, including pre-anoxic section, anaerobic section, aerobic section and anoxic section in turn along water flow direction;Pre-anoxic section is used for glycogen bacteria or denitrifying glycogen bacteria preferentially absorbs easily degradable carbon source in sewage and stores as internal carbon source, and the effluent end of anoxic section is connected with sedimentation selection screening zone;Sedimentation selection screening zone is communicated with the effluent end of anoxic section through multiple different height water inlets and corresponding effluent ports.This application sets up pre-anoxic section, anaerobic section, aerobic section and anoxic section along water flow direction, and cooperates with the upper and lower staggered arrangement of adjacent water holes, constructs periodic substrate gradient environment from satiation to hunger in space, provides continuous metabolic selection pressure for the directional enrichment and granulation of denitrifying glycogen bacteria, promotes the rapid formation and stable maintenance of structure dense, excellent settling performance aerobic granular sludge.

A method for preparing a highly branched starch of different crystal forms by using a glycogen branching enzyme. The method comprises: (1) dispersing starch in a buffer to obtain starch milk; (2) performing a sub-gelatinization pretreatment on the starch milk, and then adding a glycogen branching enzyme for enzymolysis; and (3) after the enzymolysis is completed, performing ethanol precipitation, washing, freeze-drying, grinding and sieving to obtain branched starch. By means of omitting the conventional modification step of high-temperature pre-gelatinization followed by cooling and enzyme addition, the glycogen branching enzyme is directly added at 60°C to process granular starch with different crystal form structures, which maintains the intact granular morphology of the starch, thereby achieving the effects of simultaneous enzymolysis and starch granule swelling.

This invention discloses a non-denatured cartilage nutrient ultrafine powder with joint injury repair and inflammation suppression effects, its preparation method, and its applications, relating to the fields of joint repair and medical and health care technology. The non-denatured cartilage nutrient ultrafine powder comprises the following raw materials in the indicated mass fractions: 35-50% functionalized non-denatured cartilage micropowder, 10-20% oligochitosan, 5-10% chondroitin sulfate, 5-10% glucosamine, 3-8% calcium supplement, and the remainder being excipients. The surface of the functionalized non-denatured cartilage micropowder is coated with modified hyaluronic acid, and the core layer includes plant glycogen-curcumin nanoparticles and composite particles of non-denatured cartilage micropowder. The modified hyaluronic acid contains thioether bonds. The non-denatured cartilage micropowder can effectively retain bioactive substances, providing a comprehensive nutritional substrate. The stability of curcumin after encapsulation is also improved, and the outer coating with modified hyaluronic acid can enhance targeting, enabling a highly efficient treatment step of first reducing inflammation and then repairing.