210 results about "Glycogen" patented technology

The invention discloses a wearable sweat glucose detection system based on multi-shell metal organic framework encapsulated enzyme and a preparation method of the wearable sweat glucose detection system. The detection system comprises a sweat collection and sensing module, an electrochemical detection module and an iontophoresis module, the sweat collecting and sensing module comprises a micro-fluidic channel and a screen-printed electrode; the micro-fluidic channel is used for collecting sweat; the screen-printed electrode comprises a working electrode for detecting glucose, a counter electrode and a reference electrode to form a three-electrode system; wherein the working electrode for detecting the glucose is provided with a functional layer, and the functional layer is modified by multi-shell metal organic framework layered encapsulation enzyme for detecting the glucose and chitosan for fixing the multi-shell metal organic framework layered encapsulation enzyme; the electrochemical detection module is used for obtaining electrochemical sensing detection data; and the iontophoresis module is used for outputting an adjustable constant current source to enable iontophoresis to stimulate skin to sweat. The portable long-time continuous detection of sweat glucose can be realized, and the detection method is rapid and convenient.

The invention discloses a composition with homology of medicine and food and application thereof in relieving physical fatigue, the composition is selected from at least two of ginseng, maca and green tea, and the obtained composition can synergistically regulate oxidation resistance, calcium ion metabolism and energy metabolism, synergistically improve the ATP content and glycogen level of an organism, accelerate removal of lactic acid and urea nitrogen and improve the physical fatigue. And the synergistic effect of the ginseng, the maca and the green tea is better than that of single use of the ginseng, the maca and the green tea. The composition provided by the invention is verified by a standard method of a health-care food function test and evaluation method (2023 edition), and is proved to have a remarkable effect of relieving physical fatigue.

The invention provides a hypoglycemic peptide which is prepared by taking bovine whey protein as a raw material and is stable in gastrointestinal digestion and absorption. In an in-vitro cell experiment, the peptide fragment can improve the uptake of insulin-resistant HepG2 hepatocytes and synthesize glycogen by using glucose, can promote intestinal endocrine cells NCI-H716 to secrete GLP-1, has a certain synergistic effect, and can further play the effects of promoting GLP-1 secretion and activating a GLP-1 receptor through combined use. An insulin resistance mouse model is constructed by utilizing high-fat diet, and the peptide fragment is adopted for intervention, so that the hypoglycemic peptide is further proved to have obvious effects on losing weight, reducing lipid accumulation and assisting in reducing blood fat, and meanwhile, the hypoglycemic peptide can be used for improving insulin resistance related symptoms. The milk peptide provided by the invention has gastrointestinal digestion stability and hypoglycemic activity, can be used as a functional milk base material for developing food, medicines or health care products with the effect of regulating blood sugar, and is wide in application prospect.

The invention belongs to the technical field of external medicine compositions, and particularly relates to microsphere type antibacterial gel as well as a preparation method and application thereof. The preparation comprises three parts, namely dextran microspheres wrapped by polyethylene glycol, a mixed antibacterial component and a carbomer mixed solution. The mixed antibacterial component is prepared from thymol, chlorhexidine acetate, cinnamyl aldehyde, citral, gallic acid and nano silver. Glucan is prepared into microspheres by utilizing a micro-fluidic chip technology, antibacterial components are wrapped in the microspheres, then the microspheres are compounded into the microsphere type antibacterial gel, and the gel not only shows excellent antibacterial performance and strong adhesive capacity, but also has a remarkable wound repair promoting effect; the microsphere structure can wrap and mix the antibacterial components and play a role in controlled release of the antibacterial components, so that a novel solution is provided for the medical field of antibacterial gel.

The invention relates to the technical field of microorganisms, in particular to pediococcus pentosaceus for degrading silage fumonisins and application of the pediococcus pentosaceus. The pediococcus pentosaceus is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the pediococcus pentosaceus is CGMCC No. 28710. The pediococcus pentosaceus is high in growth rate, high in acid production efficiency, good in acid resistance and salt resistance, capable of well utilizing different types of carbon sources for fermentation, high in adaptive capacity and free of virulence genes commonly having toxic effects on livestock, the pediococcus pentosaceus serves as a pasture silage additive, the fermentation type is homotype fermentation, and the application range is wide. The pH value of the silage can be rapidly reduced, generation of ammoniacal nitrogen is reduced, the content of fumonisins is efficiently reduced, the fermentation quality of the silage is improved, and wide application prospects are achieved.

The invention discloses a green low-cost sugar-based multifunctional carbon fiber and a preparation method thereof, and belongs to the technical field of carbon fiber preparation. According to the method, water-soluble saccharides, a gel monomer, a reinforcing phase and a spinning auxiliary are used as main raw materials, a carbon fiber precursor containing a multifunctional phase is prepared in situ through wet spinning and drying, and then efficient synthesis of the multifunctional carbon fiber is achieved through pre-carbonization and carbonization treatment. The multifunctional phase introduced into the prepared carbon fiber endows the carbon fiber with excellent properties such as high temperature resistance, oxidation resistance, corrosion resistance, high strength, high conductivity, high heat conductivity and high specific surface area, so that the carbon fiber is expected to be applied to multiple fields such as aerospace, automobile industry, electronic equipment, energy field, constructional engineering, medical equipment and environmental protection.

The invention relates to a novel hexahydropyrroloindole alkaloid extracted from fructus lycii, which can effectively solve the problems of extracting and preparing the novel hexahydropyrroloindole alkaloid A from the fructus lycii and realizing the application of the novel hexahydropyrroloindole alkaloid A in preparation of medicines for preventing and treating type 2 diabetes mellitus. The novel hexahydropyrroloindole alkaloid A is prepared by the following steps: sequentially eluting with water, ethanol and acetone, carrying out reduced pressure concentration on an ethanol part, extracting, stirring a sample with silica gel, carrying out gradient elution, merging the same fractions, eluting a target component, stirring the sample with silica gel, carrying out gradient elution, collecting fractions within retention time, concentrating and drying, thereby obtaining the novel hexahydropyrroloindole alkaloid A in the preparation of the medicine for preventing and treating the type 2 diabetes mellitus and the application of the novel hexahydropyrroloindole alkaloid A in the preparation of the medicine for preventing and treating the type 2 diabetes mellitus. Glucose consumption in insulin-resistant HepG2 cells can be remarkably promoted, glucose uptake capacity is improved, glycogen synthesis is promoted, activity of glycolysis key enzymes hexokinase and pyruvate kinase is enhanced, development and application prospects are wide, and economic and social benefits are remarkable.

The invention provides low-molecular-weight araboxylan as well as a preparation method and application thereof, and belongs to the technical field of biological extraction and purification. The method comprises the following steps: by taking wheat bran as a raw material, pretreating, further fully exposing arabinoxylan in wheat bran by adopting an extrusion expansion coupling steam explosion technology, hydrolyzing by xylanase, decoloring, carrying out ion exchange refining treatment, and separating and purifying components by adopting a multi-stage membrane technology, so as to obtain the arabinoxylan. And finally, preparing araboxylan with different molecular weights. Through the process, on one hand, arabinoxylan with different molecular weights is obtained, the molecular composition is optimized, on the other hand, the content and purity of arabinoxylan with small molecular weight are further improved, and the adaptability of the product is improved.

The invention discloses a method for preparing highly branched starch by using double enzymes, and belongs to the field of biologically modified starch. According to the invention, glycogen branching enzyme and cyclodextrin glucosyltransferase are adopted to synergistically modify different crystal forms of starch in a non-gelatinization swelling system (60 DEG C) to prepare highly branched starch, the reaction is carried out on the premise of ensuring the morphology of starch particles, and the later processing application characteristics are ensured, so that the highly branched starch can be fused with a traditional starch staple food carrier, and the preparation process is simple and convenient. According to the method, the enzyme modified starch and the swelling reaction are synchronously carried out, the step of cooling after high-temperature gelatinization is reduced, the production efficiency is improved, the adverse effect of high viscosity caused by high-temperature gelatinization on starch products is avoided, and the obtained product is high in yield, high in branching degree, low in digestibility and high in glucosidic bond proportion; a healthy and green new thought is provided for the field of starch deep processing.

The invention discloses a disease-resistant water-soluble fertilizer and a preparation method thereof, belonging to the technical field of water-soluble fertilizers. The preparation method comprises: preparing first nanoparticles, preparing second nanoparticles, dissolving, suspending and mixing; the preparation of the first nanoparticles comprises the following steps: preparing a chitosan aqueous solution, acid treatment, adsorption, cross-linking, fixing zinc ions and chelation; the preparation of the second nanoparticles comprises the following steps: activation, grafting and coating; the suspension comprises mixing the first nanoparticles, the second nanoparticles and water, stirring at 60-65 DEG C, adding potassium humate, potassium L-glutamate and polyvinyl pyrrolidone, and continuing to stir; the disease-resistant water-soluble fertilizer prepared by the invention has good appearance, storage resistance and dilution effect, fast dilution speed, is not prone to flatulence when stored at high temperature, does not need frequent fertilization, and can improve the growth rate and yield of crops while improving the disease resistance of crops.

The invention relates to the technical field of biology, in particular to a method for relieving high-starch-induced liver injury and glucose metabolism disorder of perches. The feed formula provided by the invention comprises the following components in percentage by weight: 20-22% of cassava starch, 2-6% of mulberry leaf polysaccharide and the like. The mulberry leaf polysaccharide and high-content starch in the feed form efficient synergy, so that the growth performance of the weever is remarkably improved, accumulation of liver glycogen and fat is effectively reduced, and the health condition of the liver is remarkably improved; meanwhile, the blood sugar regulation capability of the weever is also optimized, the insulin resistance caused by high starch diet is reduced, and the insulin sensitivity is enhanced; and by activating a PI3K / Akt signal path and other mechanisms, a glucose metabolism path is optimized, and efficient utilization of energy is promoted. The feed disclosed by the invention is economical and efficient in formula, provides solid scientific support for healthy culture of perches, and powerfully promotes sustainable development of aquaculture industry.

The invention provides a traditional Chinese medicine composition and application thereof in preparation of anti-fatigue health care wine, and relates to the technical field of traditional Chinese medicine preparation and traditional Chinese medicine health care. The traditional Chinese medicine composition takes medicinal and edible traditional Chinese medicinal materials as raw materials, is prepared from herba cistanche, radix acanthopanacis semticosi, radix asparagi, fructus phyllanthi, pollen typhae, fructus canarii, herba cichorii, folium microcotis, semen canavaliae and fructus citri, and has the effects of tonifying kidney and spleen, tonifying qi and yin and combining clearing and tonifying. The traditional Chinese medicine composition and the health-care wine prepared from the traditional Chinese medicine composition provided by the invention have an excellent anti-fatigue effect, and can be used for remarkably prolonging the weight-bearing exhaustion swimming time of mice, remarkably reducing the plasma urea nitrogen content of the mice after exercise, remarkably improving the hepatic glycogen content of the mice and remarkably improving the blood lactic acid clearance of the mice after exercise; the obvious physical fatigue relieving effect is realized. The compound is expected to be developed into anti-fatigue medicines or health-care products.

The invention belongs to the field of biological detection, and discloses an efficient and rapid concentration method and device for LPS or LAM. The inventor finds out that the non-polar macroporous resin with a specific aperture range has similar affinity adsorption efficiency to lipopolysaccharide or lipoarabinomannan, the adsorption capacity is large, the adsorption speed is high, the lipopolysaccharide can be efficiently eluted in cooperation with a specific eluent, a concentrated lipopolysaccharide to-be-detected sample is obtained, and the non-polar macroporous resin with the specific aperture range can be used for detecting the lipopolysaccharide or lipoarabinomannan in the lipopolysaccharide or lipoarabinomannan in the lipopolysaccharide or lipoarabinomannan. And a good condition is created for the detection of lipopolysaccharide or lipoarabinomannan. Efficient concentration of LAM is realized by using the method, and excellent conditions are provided for detection of mycobacterium tuberculosis.

The invention discloses a formula of a composition capable of assisting in reducing blood sugar and preventing diabetes, and relates to the field of health food, the composition has the effects of reducing blood sugar and preventing diabetes, and the composition comprises the following composition components: 0.25 to 0.37% of bitter gourd peptide, 0.018 to 0.026% of corosolic acid, 0.39 to 0.48% of mulberry leaf extract, 0.083 to 0.095% of pseudo-ginseng extract, 0.115 to 0.132% of radix polygonati officinalis extract, and 0.00003 to 0.00005% of chromium-enriched yeast. By adopting the composition formula for assisting in reducing blood sugar and preventing diabetes, the formula composition has the effects of assisting in reducing the blood sugar level and treating. Meanwhile, the composition also has a synergistic effect on prevention of diabetes caused by hyperglycemia symptoms, the components of the composition are equal-part compositions formed by physical stirring and uniform mixing, and the increase of postprandial blood sugar level can be effectively inhibited, so that the effects of assisting beta cells of a body to secrete insulin and improving the blood sugar level are achieved. And insulin can promote the utilization of sugar by cells, the synthesis of hepatic glycogen and the improvement of the metabolism of sugar, so that the effect of reducing blood sugar is finally achieved. The raw materials of the composition can be purchased and obtained in the market, the raw materials are simple and easy to obtain, the production benefit is high, and the composition is suitable for being widely used.

The invention discloses a method for reducing environmental stress of waterless keep-alive shellfish, and belongs to the technical field of keep-alive of aquatic products. The method comprises the following specific steps: cleaning caught fresh and live shellfishes, and temporarily culturing the shellfishes in a seawater temporary culture pond containing a shellfish stress inhibitor for 2-24 hours; performing cooling treatment on the temporarily cultured shellfish; draining the cooled shellfish, putting the shellfish into a packaging bag of which the inner wall is attached with a bionic gill filament film, and carrying out water-free keep-alive transportation or storage in a refrigeration environment of 2-4 DEG C; a shellfish stress inhibitor is used in the temporary rearing pond at the transportation or storage end point, and constant-speed heating is kept. Through the synergistic effect of the shellfish stress inhibitor and the bionic gill filament membrane, a more stable and suitable waterless keep-alive environment is provided for shellfishes, in the waterless keep-alive process of the shellfishes, the cell damage and death rate are reduced, and the survival rate is remarkably improved; meanwhile, glycogen consumption is reduced, the mass loss rate and the oxidative stress level of the shellfish are reduced, the survival rate of the shellfish is increased, the good texture of the shellfish is kept, and the method has important value for integrally improving the economic benefit of the waterless keep-alive shellfish.

The invention provides a domestic sewage treatment method and system based on shortcut nitrification and anaerobic ammonia oxidation of Anammox bacteria preposed alkali reduction, and relates to the technical field of sewage treatment. The method comprises the following steps: anaerobic treatment: converting an organic suspended solid into an easily degradable carbon source through hydrolytic acidification bacteria; the carbon source is absorbed by denitrifying phosphorus-accumulating bacteria and denitrifying glycogen-accumulating bacteria and is stored as an intracellular carbon source; primary anoxic treatment: reacting ammonia nitrogen with nitrite through Anammox bacteria, and synchronously reducing alkalinity; aerobic treatment: under the condition of low alkalinity, in combination with low dissolved oxygen control, Comamox bacteria oxidize ammonia nitrogen into nitrite and accumulate the nitrite; and secondary anoxic treatment: denitrifying residual oxidation nitrogen into nitrogen by using intracellular carbon through denitrifying glycogen-accumulating bacteria. Through the synergistic effect of the hydrolytic acidification bacteria, the denitrifying phosphorus-accumulating bacteria, the Anammox bacteria, the Commox bacteria and the denitrifying glycogen-accumulating bacteria, efficient nitrogen and phosphorus removal is realized.

The invention belongs to the technical field of medicines, and particularly relates to production and application of a glucagon-like peptide-1 analogue-coated bacterial outer membrane vesicle which is mainly applied to treatment of type 2 diabetes mellitus (T2DM) and obesity. The glucagon-like peptide-1 (GLP1) analogue-coated bacterial outer membrane vesicle load gene has a coding sequence as shown in the GLP1 analogue of SEQ ID NO: 1. The bacterial outer membrane vesicles wrapping the GLP1 analogue, which are successfully prepared by the invention, have potential clinical applicability and research value in treatment of type 2 diabetes mellitus (T2DM) and obesity.

The invention provides a lysis binding solution, an extraction kit and a detection method for extracting residual DNA of host cells, the lysis binding solution comprises guanidine hydrochloride, PEG6000, tRNA, glycogen and isopropanol, the concentration of guanidine hydrochloride is 2.0-2.5 M, the concentration of PEG6000 is 1%-10% (m / v), and the concentration of isopropanol is 50-60%. According to the present invention, the host cell residual DNA extraction is performed by using the lysis binding liquid, such that the compatibility between the reagent and different sample types can be significantly enhanced, the sample pre-dilution is reduced, and the matrix interference is reduced so as to improve the extraction efficiency and the extraction accuracy of the residual DNA, and effectively overcome the limitation of the commercial kit.

The invention discloses an actinomycete strain YC3041 and application of the actinomycete strain YC3041 in preparation of a biological preparation for preventing and treating tobacco phytophthora root rot. The actinomycete strain YC3041 is classified and named as Streptomyces pratensis; the strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC), and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou; the preservation date is July 4, 2024; the preservation number of the strain is GDMCC No: 64828. Physiological and biochemical tests show that the YC3041 has the capabilities of gelatin liquefaction, milk dissolution and the like, can utilize various glycogens, and has the potassium dissolving capability. The YC3041 also has a certain inhibition effect on other pathogenic bacteria, such as YC1026 and YC1265. The invention provides a novel efficient biocontrol strain YC3041 for biological control of phytophthora root rot of tobacco, and the strain has a wide application prospect.

The present invention belongs to the technical field of medicine and food homologous compositions, and discloses a composition for enhancing exercise endurance and its applications. It is found that the composition has the following effects: (1) After 30 minutes of intervention with a high dose of the composition, exercise endurance can be significantly enhanced, and it can affect exercise endurance by regulating the levels of hormones such as testosterone and cortisol. (2) Intervention with the composition for 14 days can significantly prolong the swimming time to exhaustion under negative gravity in mice, reduce the accumulation of harmful metabolites, thereby increasing the body's energy metabolism and reducing the degree of fatigue of the body. (3) Intervention with a high dose of the composition for 28 days can significantly increase exercise endurance, and by increasing the muscle glycogen reserve and reducing the accumulation of harmful metabolites such as urea nitrogen and lactic acid, the effect of improving exercise endurance is achieved.

The invention discloses a composite water-soluble immunologic adjuvant and a preparation method thereof. The composite water-soluble immunologic adjuvant is prepared from the following components in parts by weight: 18-22 parts of phosphate buffer salt solution dry powder, 1-3 parts of sodium ascorbate, 8-12 parts of beta-glucan, 4-8 parts of xanthan gum and carrageenan mixture, 8-14 parts of plant extract, 2-5 parts of plant polysaccharide and 1930-1960 parts of ultrapure water. According to the adjuvant, the problem that a specific antibody is generated for the adjuvant is effectively avoided, the detection rate of positive clone is remarkably increased, and the possibility of obtaining a high-quality paired antibody is increased. The mixing process of the adjuvant and the water-soluble antigen is simple and does not need emulsification. The native conformation of the antigen is retained, and the generation of antibodies aiming at conformation-dependent epitopes is facilitated. Compared with traditional Freund's adjuvants and incomplete Freund's adjuvants, the composite water-soluble adjuvant has the advantages that the use amount of antigens is small, the immune cycle is shortened, efficient immune response and antibody generation can be quickly stimulated, and the composite water-soluble adjuvant has remarkable competitive advantages.

The invention discloses a method for preparing highly branched starch with different crystal forms from glycogen branching enzyme, and belongs to the field of biologically modified starch. The method comprises the following steps: (1) dispersing starch in a buffer solution to obtain starch milk; (2) carrying out sub-gelatinization pretreatment on the starch milk, and then adding glycogen branching enzyme to carry out enzymolysis reaction; and (3) after the enzymolysis is finished, carrying out alcohol precipitation, washing, freeze drying, grinding and sieving to obtain the branched starch. The conventional modification step of cooling and adding enzyme after high-temperature pre-gelatinization treatment is omitted, the effect that glycogen branching enzyme is directly added at 60 DEG C to act on granular starch with different crystal structures is realized, the complete granular form of the starch is ensured, the post-processing characteristics are ensured, and the effect of synchronously performing enzymolysis reaction and starch granule swelling is realized; the problems caused by treating high viscosity of starch are avoided while the energy consumption is reduced, and the adverse effects of high-temperature gelatinization on the texture and properties of starch products are avoided.

The present invention relates to a process for purifying phycocyanins from a solution comprising phycocyanins and glycogen, said phycocyanins being produced by microorganisms belonging to the order Cyanidiales, characterized in that it comprises (i) an enzymatic degradation step of glycogen with an enzyme suitable for degrading glycogen at a pH below 6 and a reaction temperature below 40°C, said enzyme being selected from a pectinase, a glucoamylase, or a mixture of a pectinase and a pullulanase, and (ii) a step of separating phycocyanins from the glycogen degradation products.

The invention belongs to the technical field of protein peptide preparation, and particularly relates to wheat oligopeptide with an anti-fatigue effect and a preparation method of the wheat oligopeptide. According to the method, the raw materials are subjected to enzymolysis by using the first compound enzyme containing bacillus subtilis protease and bromelain and the second compound enzyme containing trypsin, flavourzyme and papain step by step, and treatment of centrifugation, ultrafiltration and the like is combined, so that the yield and the taste of the product are improved, and the proportion of small molecular weight peptide in the product is high. Therefore, the wheat oligopeptide prepared by the method is high in yield and good in taste, not only is beneficial to improving the endurance of the body and prolonging the exercise time, but also can improve the energy metabolism condition in the body, delay the accumulation of lactic acid, urea nitrogen and creatine kinase in the body after exercise and increase the glycogen storage amount, and macroscopically shows certain anti-fatigue ability. The technical scheme of the invention has strong practicability, is beneficial to industrial production, and can be applied to preparation of anti-fatigue functional food.

The invention discloses chitinase derived from myxococcus flavus and application of the chitinase. The amino acid sequence of the chitinase ChitM disclosed by the invention is shown as the 22 -406 amino acid sequence of SEQ ID NO. 2. The chitinase ChitM disclosed by the invention has a remarkable splitting action on plant pathogenic fungi such as banana disc elongispora, banana fusarium oxysporum, tomato fusarium oxysporum, magnaporthe oryzae and botrytis cinerea, and when the encoding gene of the chitinase ChitM is transferred into arabidopsis thaliana, the resistance of the arabidopsis thaliana with the transferred ChitM gene to botrytis cinerea is obviously improved compared with that of wild arabidopsis thaliana; besides, the activity of ChitM is compared with that of commercial chitinase C.Chit, it is found that when the substrates are chitin and shrimp shells, the enzyme activity of the two is not obviously different, when the substrates are chitosan, the activity of ChitM is obviously higher than that of C.Chit, and when the substrates are soluble chitosan and colloidal chitin, the enzyme activity of C.Chit is obviously higher than that of ChitM, and when the substrates are soluble chitosan and colloidal chitin, the enzyme activity of ChitM is obviously higher than that of C.Chit. The results show that the enzyme has great application potential in the aspects of biological prevention and control of plant fungal diseases, disease resistance, breeding and environmental protection.

This invention belongs to the field of heavy metal removal technology, specifically disclosing an extract from the leaves of a Solanaceae plant, its extraction method, and its application. The extraction method includes the following steps: S1, mixing tomato and pepper leaves, chopping them to obtain a preliminary mixture; S2, mixing a compound microbial agent with the preliminary mixture obtained in S1, fermenting, sterilizing, centrifuging, filtering through a 0.45μm filter membrane, discarding the precipitate, and obtaining a fermented preliminary mixture; S3, concentrating the fermented preliminary mixture obtained in S2 under reduced pressure to obtain a concentrated fermentation broth; S4, adding sodium carboxymethyl cellulose to the concentrated fermentation broth obtained in S3, stirring evenly, and freeze-drying to obtain the Solanaceae plant leaf extract. This Solanaceae plant leaf extract can effectively remove cadmium from aquatic products, aiding in the growth recovery of aquatic products after cadmium stress, and can also effectively maintain the pH value of white clam muscle, reducing lactic acid accumulation and glycogen consumption, and maintaining the freshness of the meat.

The invention discloses a camelina sativa seed meal oligosaccharide extract capable of regulating blood sugar and a preparation method of the camelina sativa seed meal oligosaccharide extract, and the camelina sativa seed meal oligosaccharide extract is extracted from camelina sativa seed meal through fermentation, heating extraction, cooling alcohol precipitation and enzymolysis. Comprising a camelina seed meal oligosaccharide extract and a cortex fraxini iridoid component extract. The cortex fraxini iridoid component extract is obtained through microwave-assisted enzymolysis extraction and purification; the invention further discloses application of the blood sugar reducing composition in blood sugar reducing food, medicine or health care products. The camelina seed meal oligosaccharide extract, the cortex fraxini iridoid component extract and the hypoglycemic composition have inhibition ability on alpha-glucosidase and alpha-amylase, can improve glucose consumption, glycogen content and glycolysis key enzyme activity, effectively improve insulin resistance, improve oxidative stress, regulate intestinal flora structure, and improve the blood sugar level, so that the hypoglycemic effect of the composition is improved. The traditional Chinese medicine is safe and non-toxic and has a good effect on treating diabetes and complications.

The invention belongs to the field of biomedicine, and particularly relates to a construction method of an in-situ biological 3D printing artificial liver patch for treating hepatic failure. The main component of the artificial liver patch disclosed by the invention is composite hydrogel, and the composite hydrogel comprises hyaluronic acid (HA), gelatin (Gelatin), sodium alginate (sodium alginate) and methacrylated gelatin (GelMA). The composite hydrogel has good 3D printing performance, large-scale construction of the artificial liver patch can be achieved through biological 3D printing, and a potential solution is provided for transplantable biological artificial liver tissue. The artificial liver patch adopts an extrusion type printing mode, the forming speed is high, internal liver cells can still keep high activity, reconstruction of liver basic functions (glycogen storage, drug metabolism, secretory protein synthesis and the like) can be guaranteed, and the artificial liver patch has important potential in treatment of liver failure diseases.

The application discloses a kind of immortalization human umbilical cord mesenchymal stem cell NOTCH1-LNR overexpression stable cell line construction method and its application in liver differentiation, it is related to immortalized cell technical field.The application first connects target gene to vector, and the slow virus plasmid containing target gene is constructed, the slow virus plasmid containing target gene is introduced into 293T cell, and high titer slow virus containing target gene is generated;Immortalized human umbilical cord mesenchymal stem cell is infected with slow virus, and mixed clone cell strain is screened, and the immortalized human umbilical cord mesenchymal stem cell NOTCH1-LNR overexpression stable cell line is obtained.The application proves that the differentiation efficiency of immortalized human umbilical cord mesenchymal stem cell NOTCH1-LNR overexpression can effectively improve liver-like cell to glycogen staining and Western blotting experiment.This research result is expected to provide new technical thought for the next step development to improve the yield of MSCs liver differentiation.

The present invention discloses a highly active ginseng fermented product and its application. Ginseng is fermented using a composite strain composed of independently screened plant lactobacillus Byf‑r‑01 and grape juice spore Hansen yeast Byf‑j‑01. The composite strain metabolic enzyme system is highly complementary, and the prototype ginsenosides are efficiently converted into rare ginsenosides in a collaborative manner, while simultaneously improving the content of polyphenols, flavonoids, alkaloids and volatile oils. The present invention adds chitosan-polyaspartic acid complex to neutralize lactic acid and maintain pH stability. At the same time, it promotes the proliferation of composite strains as a nitrogen source, significantly improves strain biomass and the activity of saponin-related converting enzymes, and promotes the conversion of ginsenosides. The highly active ginseng fermented product prepared by the present invention has both strong antioxidant and anti-fatigue functions. It has been confirmed by animal experiments that it can prolong the exhaustive swimming time of mice, reduce blood urea nitrogen levels, increase liver / muscle glycogen reserves, and significantly alleviate lactic acid accumulation after exercise, providing a new path for the development of high value-added functional products for ginseng.