139 results about "Glycogen" patented technology

The invention provides a hypoglycemic peptide which is prepared by taking bovine whey protein as a raw material and is stable in gastrointestinal digestion and absorption. In an in-vitro cell experiment, the peptide fragment can improve the uptake of insulin-resistant HepG2 hepatocytes and synthesize glycogen by using glucose, can promote intestinal endocrine cells NCI-H716 to secrete GLP-1, has a certain synergistic effect, and can further play the effects of promoting GLP-1 secretion and activating a GLP-1 receptor through combined use. An insulin resistance mouse model is constructed by utilizing high-fat diet, and the peptide fragment is adopted for intervention, so that the hypoglycemic peptide is further proved to have obvious effects on losing weight, reducing lipid accumulation and assisting in reducing blood fat, and meanwhile, the hypoglycemic peptide can be used for improving insulin resistance related symptoms. The milk peptide provided by the invention has gastrointestinal digestion stability and hypoglycemic activity, can be used as a functional milk base material for developing food, medicines or health care products with the effect of regulating blood sugar, and is wide in application prospect.

The invention relates to the technical field of biology, in particular to a method for relieving high-starch-induced liver injury and glucose metabolism disorder of perches. The feed formula provided by the invention comprises the following components in percentage by weight: 20-22% of cassava starch, 2-6% of mulberry leaf polysaccharide and the like. The mulberry leaf polysaccharide and high-content starch in the feed form efficient synergy, so that the growth performance of the weever is remarkably improved, accumulation of liver glycogen and fat is effectively reduced, and the health condition of the liver is remarkably improved; meanwhile, the blood sugar regulation capability of the weever is also optimized, the insulin resistance caused by high starch diet is reduced, and the insulin sensitivity is enhanced; and by activating a PI3K / Akt signal path and other mechanisms, a glucose metabolism path is optimized, and efficient utilization of energy is promoted. The feed disclosed by the invention is economical and efficient in formula, provides solid scientific support for healthy culture of perches, and powerfully promotes sustainable development of aquaculture industry.

The invention provides a traditional Chinese medicine composition and application thereof in preparation of anti-fatigue health care wine, and relates to the technical field of traditional Chinese medicine preparation and traditional Chinese medicine health care. The traditional Chinese medicine composition takes medicinal and edible traditional Chinese medicinal materials as raw materials, is prepared from herba cistanche, radix acanthopanacis semticosi, radix asparagi, fructus phyllanthi, pollen typhae, fructus canarii, herba cichorii, folium microcotis, semen canavaliae and fructus citri, and has the effects of tonifying kidney and spleen, tonifying qi and yin and combining clearing and tonifying. The traditional Chinese medicine composition and the health-care wine prepared from the traditional Chinese medicine composition provided by the invention have an excellent anti-fatigue effect, and can be used for remarkably prolonging the weight-bearing exhaustion swimming time of mice, remarkably reducing the plasma urea nitrogen content of the mice after exercise, remarkably improving the hepatic glycogen content of the mice and remarkably improving the blood lactic acid clearance of the mice after exercise; the obvious physical fatigue relieving effect is realized. The compound is expected to be developed into anti-fatigue medicines or health-care products.

The invention discloses a method for reducing environmental stress of waterless keep-alive shellfish, and belongs to the technical field of keep-alive of aquatic products. The method comprises the following specific steps: cleaning caught fresh and live shellfishes, and temporarily culturing the shellfishes in a seawater temporary culture pond containing a shellfish stress inhibitor for 2-24 hours; performing cooling treatment on the temporarily cultured shellfish; draining the cooled shellfish, putting the shellfish into a packaging bag of which the inner wall is attached with a bionic gill filament film, and carrying out water-free keep-alive transportation or storage in a refrigeration environment of 2-4 DEG C; a shellfish stress inhibitor is used in the temporary rearing pond at the transportation or storage end point, and constant-speed heating is kept. Through the synergistic effect of the shellfish stress inhibitor and the bionic gill filament membrane, a more stable and suitable waterless keep-alive environment is provided for shellfishes, in the waterless keep-alive process of the shellfishes, the cell damage and death rate are reduced, and the survival rate is remarkably improved; meanwhile, glycogen consumption is reduced, the mass loss rate and the oxidative stress level of the shellfish are reduced, the survival rate of the shellfish is increased, the good texture of the shellfish is kept, and the method has important value for integrally improving the economic benefit of the waterless keep-alive shellfish.

The invention provides a domestic sewage treatment method and system based on shortcut nitrification and anaerobic ammonia oxidation of Anammox bacteria preposed alkali reduction, and relates to the technical field of sewage treatment. The method comprises the following steps: anaerobic treatment: converting an organic suspended solid into an easily degradable carbon source through hydrolytic acidification bacteria; the carbon source is absorbed by denitrifying phosphorus-accumulating bacteria and denitrifying glycogen-accumulating bacteria and is stored as an intracellular carbon source; primary anoxic treatment: reacting ammonia nitrogen with nitrite through Anammox bacteria, and synchronously reducing alkalinity; aerobic treatment: under the condition of low alkalinity, in combination with low dissolved oxygen control, Comamox bacteria oxidize ammonia nitrogen into nitrite and accumulate the nitrite; and secondary anoxic treatment: denitrifying residual oxidation nitrogen into nitrogen by using intracellular carbon through denitrifying glycogen-accumulating bacteria. Through the synergistic effect of the hydrolytic acidification bacteria, the denitrifying phosphorus-accumulating bacteria, the Anammox bacteria, the Commox bacteria and the denitrifying glycogen-accumulating bacteria, efficient nitrogen and phosphorus removal is realized.

The invention provides a lysis binding solution, an extraction kit and a detection method for extracting residual DNA of host cells, the lysis binding solution comprises guanidine hydrochloride, PEG6000, tRNA, glycogen and isopropanol, the concentration of guanidine hydrochloride is 2.0-2.5 M, the concentration of PEG6000 is 1%-10% (m / v), and the concentration of isopropanol is 50-60%. According to the present invention, the host cell residual DNA extraction is performed by using the lysis binding liquid, such that the compatibility between the reagent and different sample types can be significantly enhanced, the sample pre-dilution is reduced, and the matrix interference is reduced so as to improve the extraction efficiency and the extraction accuracy of the residual DNA, and effectively overcome the limitation of the commercial kit.

The invention discloses an actinomycete strain YC3041 and application of the actinomycete strain YC3041 in preparation of a biological preparation for preventing and treating tobacco phytophthora root rot. The actinomycete strain YC3041 is classified and named as Streptomyces pratensis; the strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC), and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou; the preservation date is July 4, 2024; the preservation number of the strain is GDMCC No: 64828. Physiological and biochemical tests show that the YC3041 has the capabilities of gelatin liquefaction, milk dissolution and the like, can utilize various glycogens, and has the potassium dissolving capability. The YC3041 also has a certain inhibition effect on other pathogenic bacteria, such as YC1026 and YC1265. The invention provides a novel efficient biocontrol strain YC3041 for biological control of phytophthora root rot of tobacco, and the strain has a wide application prospect.

The present invention belongs to the technical field of medicine and food homologous compositions, and discloses a composition for enhancing exercise endurance and its applications. It is found that the composition has the following effects: (1) After 30 minutes of intervention with a high dose of the composition, exercise endurance can be significantly enhanced, and it can affect exercise endurance by regulating the levels of hormones such as testosterone and cortisol. (2) Intervention with the composition for 14 days can significantly prolong the swimming time to exhaustion under negative gravity in mice, reduce the accumulation of harmful metabolites, thereby increasing the body's energy metabolism and reducing the degree of fatigue of the body. (3) Intervention with a high dose of the composition for 28 days can significantly increase exercise endurance, and by increasing the muscle glycogen reserve and reducing the accumulation of harmful metabolites such as urea nitrogen and lactic acid, the effect of improving exercise endurance is achieved.

The present invention relates to a process for purifying phycocyanins from a solution comprising phycocyanins and glycogen, said phycocyanins being produced by microorganisms belonging to the order Cyanidiales, characterized in that it comprises (i) an enzymatic degradation step of glycogen with an enzyme suitable for degrading glycogen at a pH below 6 and a reaction temperature below 40°C, said enzyme being selected from a pectinase, a glucoamylase, or a mixture of a pectinase and a pullulanase, and (ii) a step of separating phycocyanins from the glycogen degradation products.

The invention belongs to the technical field of protein peptide preparation, and particularly relates to wheat oligopeptide with an anti-fatigue effect and a preparation method of the wheat oligopeptide. According to the method, the raw materials are subjected to enzymolysis by using the first compound enzyme containing bacillus subtilis protease and bromelain and the second compound enzyme containing trypsin, flavourzyme and papain step by step, and treatment of centrifugation, ultrafiltration and the like is combined, so that the yield and the taste of the product are improved, and the proportion of small molecular weight peptide in the product is high. Therefore, the wheat oligopeptide prepared by the method is high in yield and good in taste, not only is beneficial to improving the endurance of the body and prolonging the exercise time, but also can improve the energy metabolism condition in the body, delay the accumulation of lactic acid, urea nitrogen and creatine kinase in the body after exercise and increase the glycogen storage amount, and macroscopically shows certain anti-fatigue ability. The technical scheme of the invention has strong practicability, is beneficial to industrial production, and can be applied to preparation of anti-fatigue functional food.

This invention belongs to the field of heavy metal removal technology, specifically disclosing an extract from the leaves of a Solanaceae plant, its extraction method, and its application. The extraction method includes the following steps: S1, mixing tomato and pepper leaves, chopping them to obtain a preliminary mixture; S2, mixing a compound microbial agent with the preliminary mixture obtained in S1, fermenting, sterilizing, centrifuging, filtering through a 0.45μm filter membrane, discarding the precipitate, and obtaining a fermented preliminary mixture; S3, concentrating the fermented preliminary mixture obtained in S2 under reduced pressure to obtain a concentrated fermentation broth; S4, adding sodium carboxymethyl cellulose to the concentrated fermentation broth obtained in S3, stirring evenly, and freeze-drying to obtain the Solanaceae plant leaf extract. This Solanaceae plant leaf extract can effectively remove cadmium from aquatic products, aiding in the growth recovery of aquatic products after cadmium stress, and can also effectively maintain the pH value of white clam muscle, reducing lactic acid accumulation and glycogen consumption, and maintaining the freshness of the meat.

The invention discloses a camelina sativa seed meal oligosaccharide extract capable of regulating blood sugar and a preparation method of the camelina sativa seed meal oligosaccharide extract, and the camelina sativa seed meal oligosaccharide extract is extracted from camelina sativa seed meal through fermentation, heating extraction, cooling alcohol precipitation and enzymolysis. Comprising a camelina seed meal oligosaccharide extract and a cortex fraxini iridoid component extract. The cortex fraxini iridoid component extract is obtained through microwave-assisted enzymolysis extraction and purification; the invention further discloses application of the blood sugar reducing composition in blood sugar reducing food, medicine or health care products. The camelina seed meal oligosaccharide extract, the cortex fraxini iridoid component extract and the hypoglycemic composition have inhibition ability on alpha-glucosidase and alpha-amylase, can improve glucose consumption, glycogen content and glycolysis key enzyme activity, effectively improve insulin resistance, improve oxidative stress, regulate intestinal flora structure, and improve the blood sugar level, so that the hypoglycemic effect of the composition is improved. The traditional Chinese medicine is safe and non-toxic and has a good effect on treating diabetes and complications.

The invention belongs to the field of biomedicine, and particularly relates to a construction method of an in-situ biological 3D printing artificial liver patch for treating hepatic failure. The main component of the artificial liver patch disclosed by the invention is composite hydrogel, and the composite hydrogel comprises hyaluronic acid (HA), gelatin (Gelatin), sodium alginate (sodium alginate) and methacrylated gelatin (GelMA). The composite hydrogel has good 3D printing performance, large-scale construction of the artificial liver patch can be achieved through biological 3D printing, and a potential solution is provided for transplantable biological artificial liver tissue. The artificial liver patch adopts an extrusion type printing mode, the forming speed is high, internal liver cells can still keep high activity, reconstruction of liver basic functions (glycogen storage, drug metabolism, secretory protein synthesis and the like) can be guaranteed, and the artificial liver patch has important potential in treatment of liver failure diseases.

The application discloses a kind of immortalization human umbilical cord mesenchymal stem cell NOTCH1-LNR overexpression stable cell line construction method and its application in liver differentiation, it is related to immortalized cell technical field.The application first connects target gene to vector, and the slow virus plasmid containing target gene is constructed, the slow virus plasmid containing target gene is introduced into 293T cell, and high titer slow virus containing target gene is generated;Immortalized human umbilical cord mesenchymal stem cell is infected with slow virus, and mixed clone cell strain is screened, and the immortalized human umbilical cord mesenchymal stem cell NOTCH1-LNR overexpression stable cell line is obtained.The application proves that the differentiation efficiency of immortalized human umbilical cord mesenchymal stem cell NOTCH1-LNR overexpression can effectively improve liver-like cell to glycogen staining and Western blotting experiment.This research result is expected to provide new technical thought for the next step development to improve the yield of MSCs liver differentiation.

The present invention discloses a highly active ginseng fermented product and its application. Ginseng is fermented using a composite strain composed of independently screened plant lactobacillus Byf‑r‑01 and grape juice spore Hansen yeast Byf‑j‑01. The composite strain metabolic enzyme system is highly complementary, and the prototype ginsenosides are efficiently converted into rare ginsenosides in a collaborative manner, while simultaneously improving the content of polyphenols, flavonoids, alkaloids and volatile oils. The present invention adds chitosan-polyaspartic acid complex to neutralize lactic acid and maintain pH stability. At the same time, it promotes the proliferation of composite strains as a nitrogen source, significantly improves strain biomass and the activity of saponin-related converting enzymes, and promotes the conversion of ginsenosides. The highly active ginseng fermented product prepared by the present invention has both strong antioxidant and anti-fatigue functions. It has been confirmed by animal experiments that it can prolong the exhaustive swimming time of mice, reduce blood urea nitrogen levels, increase liver / muscle glycogen reserves, and significantly alleviate lactic acid accumulation after exercise, providing a new path for the development of high value-added functional products for ginseng.

The invention discloses a hexapeptide VA6 with an anti-fatigue function as well as a preparation method and application thereof, and belongs to the technical field of biology. The hexapeptide VA6 is screened from pacific salmon protamine enzymatic hydrolysate, the amino acid sequence is VGVVVA, the binding capacity with AMPK and LDH is high, the liver glycogen content of a fatigue model mouse can be remarkably increased, the serum urea nitrogen content of the fatigue model mouse can be remarkably reduced, the fatigue phenotype of the fatigue model mouse can be remarkably relieved, and the load swimming time of the fatigue model mouse can be remarkably prolonged; the food has an anti-fatigue function and can be applied to development of functional food for relieving fatigue.

The invention provides a respiratory-gating-free free-breathing liver glycogen quantitative magnetic resonance imaging method and system, and the method comprises the steps: applying a chemical exchange saturation transfer imaging sequence to a liver region of a subject, and the imaging sequence comprises saturation pulses of a plurality of saturation frequency deviation points; a radial blade type k-space acquisition strategy is adopted to acquire magnetic resonance signal data, the radial blade type k-space acquisition strategy comprises a plurality of blades, each blade is composed of a group of parallel k-space lines, and different blades are rotationally distributed by taking the k-space center as a rotating shaft; performing image reconstruction on the acquired k space data to obtain magnetic resonance images under different saturation frequency deviations; and performing fitting analysis on the magnetic resonance image by adopting a multi-pool Lorentz model to obtain quantitative information of the liver glycogen. According to the method, the interference of respiratory movement on the CESTMRI data acquisition process can be eliminated, so that the accurate quantification of the liver glycogen in the free respiration state is realized.

Provided herein is a noninvasive method for measuring pH, glucose and glycogen levels using a single acquisition method, as well as systems for performing the method. This method for glycogen / glucose (and pH) detection has high specificity enabled by superior chemical shift separation in high-strength magnetic fields. The methods have applications in detecting changing tumor microenvironments in cancerous cells for measuring important metrics for noninvasive and quantitative metabolic profiling.

The invention relates to the technical field of biological treatment of urban domestic sewage, in particular to a stable deep denitrification method for aerobic granular sludge based on a reactor, which is characterized in that the reactor is operated by adopting a gradient water inlet mode combined with a dissolved oxygen regulation and control strategy, that is, short-time rapid water inlet is applied before slow water inlet to create an unbalanced external carbon source supply condition; internal carbon source storage and enrichment of slow-growing microorganisms such as phosphorus-accumulating bacteria and glycogen-accumulating bacteria are promoted, so that the stability of the particle structure is enhanced; the dissolved oxygen of the reactor main body is gradually reduced to 2 mg / L so as to reduce the inward permeation of the dissolved oxygen, increase the internal anaerobic zone and improve the denitrification rate, and meanwhile, a transient high oxygen exposure condition is set at the tail end of the aerobic stage so as to form high hydraulic shear force to further maintain the stability of the particles. The structure regularity, the mechanical strength and the denitrification performance of the aerobic granular sludge are remarkably improved, the average removal rate of ammonia nitrogen and the average removal rate of total nitrogen are stabilized at 97.84% and 88.87% or above respectively, the synchronous nitrification endogenous denitrification efficiency reaches 92.10% or above, and long-term stable operation of the system is guaranteed.

The present invention relates to a treatment method for degrading glycogens present in biomass of unicellular red algae (URA), in particular of the genus Galdiella, and to the products thus obtained.

The invention relates to the technical field of microbial fertilizer preparation, and provides a coating method for improving the coating rate of microbial fertilizer particles in order to solve the problem of poor affinity between a hydrophilic material and hydrophobic microbial fertilizer particles and improve the coating rate of the hydrophilic material as a film layer to the microbial fertilizer particles. The fertilizer is composed of a microbial fertilizer particle core layer and a modified film layer coating the outer layer of the fertilizer, and the mass ratio of the core layer to the film layer is 100: (0.5-10). Wherein the core layer can be made of trichoderma harzianum, penicillium baylii or aspergillus niger microbial fertilizer particles; the raw material of the film layer can be composed of the following components: polyvinyl alcohol (PVA), a hydrophilic material (such as starch, glucose, maltose or dextrin) capable of providing glycogen, a surfactant capable of changing the surface tension of water, a wetting agent, a dispersing agent (such as lauryl sodium sulfate, disodium methylene dinaphthalene sulfonate, sodium dodecyl benzene sulfonate or fatty acid salt) and the like. According to the coating method for improving the coating rate of the microbial fertilizer particles, the higher coating rate is achieved, the coated microbial fertilizer further has the longer container life and the higher pressure resistance, the looseness problem is greatly solved, the problems that traditional microbial fertilizer particles are prone to inactivation, breaking and loosening are solved, and the yield of the microbial fertilizer particles is improved. Good popularization and application values are realized.

Use of dimethyl sulfoxide (DMSO) or glycofurol or their combination as an additive in a solution comprising a solvent and at least one active ingredient selected from melatonin and / or an antioxidant metabolite, derivative or analogue thereof, for prolonging stability and / or shelf life of said at least one active ingredient in said solution by retarding a decomposition of said at least one active ingredient comprised in said solution.

The present application relates to the technical field of biotechnology, and particularly relates to a tagatose-6-phosphate phosphatase mutant and a method for synthesizing tagatose from scratch. The method provided by the present application is a one-pot method for biosynthesizing D-tagatose by using starch or dextrin as a substrate, adopting a multi-enzyme cascade reaction system composed of a tagatose-6-phosphate phosphatase mutant, a glycogen-debranching enzyme, an alpha-glucan phosphorylase, a phosphoglucomutase, a glucose-6-phosphate isomerase, a D-tagatose-6-phosphate-3-epimerase and a 4-alpha-glucan transferase. The method can prepare tagatose from ordinary and inexpensive starting materials (starch or dextrin) at a high yield, improves the overall conversion rate of the system and the final yield of tagatose, provides a new technical approach for realizing low-cost and green production of tagatose, and has the advantages of high yield, low cost and being more suitable for industrial production.

This disclosure relates to the use of glycogen phosphatase B agonists in the treatment of neurodegenerative diseases. Specifically, this disclosure provides the use of glycogen phosphatase B agonists (preferably adenosine monophosphate) in the preparation of medicaments for the prevention or treatment of neurodegenerative diseases. This disclosure demonstrates that glycogen phosphatase B agonists (AMPs) hold promise as a safe and effective treatment option for patients with neurodegenerative diseases.

The invention discloses an application of miR-146b-3p inhibitor in improving sheep embryo implantation abnormity caused by LPS (Lipopolysaccharide), and belongs to the technical field of biology, and the sequence of the miR-146b-3p inhibitor is shown as SEQ ID No.1. The invention further discloses a preparation method of the miR-146b-3p inhibitor. LPS can cause sheep endometrial epithelial cell inflammatory factor abnormity, adhesion molecule expression change, trophoblast sphere adhesion growth inhibition and miR-146b-3p expression increase, miR-146b-3p inhibits GYS2 gene expression in a targeted manner, endometrial glycogen synthesis is reduced, and embryo implantation is affected. The miR-146b-3p inhibitor can be used for rescuing GYS2 expression reduction caused by LPS (Lipopolysaccharide), relieving glycogen synthesis reduction and expression inhibition of an attachment key gene, promoting sheep embryo attachment and reducing the embryo loss rate. The invention further provides a reagent containing the inhibitor, a reagent for overexpressing the GYS2 gene, a method for promoting embryo implantation and related products, a new direction is provided for improving the reproductive performance of sheep, and the inhibitor has important value and significance for the sheep raising industry.

The invention relates to the technical field of food science and biology, and discloses a multi-element plant active peptide with a synergistic anti-fatigue effect as well as a preparation method and application of the multi-element plant active peptide. According to the preparation method, soybean protein, wheat protein and pollen pini in a specific mass ratio are used as raw materials, and enzymolysis treatment is performed by adopting a composite enzymolysis technology in combination with multi-frequency pulse ultrasound assistance. The multi-element plant active peptide prepared by the method has a specific molecular weight distribution range (600-1500Da), and the branched chain amino acid content of the multi-element plant active peptide is high. Mouse experiments show that the active peptide can significantly improve the endurance, muscle glycogen reserve and liver SOD activity of mice, and the synergistic anti-fatigue effect of the active peptide is verified.

The present invention belongs to the technical field of dairy products, and particularly relates to the preparation and application of camel milk hypoglycemic peptides with DPP-IV inhibitory activity and GPR-40 agonist activity. A method for preparing hypoglycemic peptides from camel milk is provided. By using the method of molecular docking, endogenous camel milk peptide sequences with the ability to inhibit DPP-IV and activate GPR-40 are screened, including AGM and KPAF. It is confirmed in vitro in HepG2 hepatocytes that the endogenous camel milk peptides can improve the uptake of insulin resistance and utilize glucose to synthesize glycogen; at the in vivo level, it is confirmed that the endogenous camel milk peptides have a certain improvement effect on insulin-resistant mice, and at the same time have the effects of inhibiting serum DPP-IV and activating the expression of colon GPR-40. Therefore, the endogenous camel milk peptides of the present invention have hypoglycemic activity, can relieve insulin resistance diseases, and provide a scientific basis for the development of health foods, drugs with blood sugar-regulating effects and industrial production.

The invention relates to application of theaflavin as a UGP2 inhibitor and application of theaflavin in preparation of a medicine for preventing and / or treating hepatocellular carcinoma, and belongs to the technical field of biology. According to the invention, the monomeric compound theaflavin derived from a natural product can be used as a UGP2 direct targeting inhibitor for the first time, and is used for preparing a liver cancer treatment medicine for regulating and controlling glucose metabolism reprogramming. The theaflavin can be specifically and directly combined at a UGP2 active site in a targeting manner through high activity to inhibit the activity of the UGP2, is the first specific inhibitor which takes the UGP2 as a target spot and is reported at present, and can be used for remarkably inhibiting tumor phenotypes such as proliferation, migration and invasion of liver cancer cells by inhibiting UGP2-mediated UDPG synthesis and intracellular glycogen accumulation.

The present invention relates to the field of sewage biological treatment technology, and specifically to a method and device for enhancing carbon source storage in activated sludge through non-steady-state carbon supply. Specifically, the method comprises: inoculating activated sludge into a reactor, starting with domestic sewage and nitrate wastewater containing different COD concentrations as influent, pumping the activated sludge into the reactor via a peristaltic pump, and sequentially performing anaerobic stirring and anoxic stirring; the reactor operates for four cycles per day; the carbon source content in the activated sludge is regularly tested; a fluctuation of ±10% in the influent COD is the optimal non-steady-state carbon supply condition for the reactor, which can enable the carbon source content in the activated sludge of the reactor to reach a peak value. The present invention uses non-steady-state carbon supply control means to provide targeted stimulation to glycogen-accumulating bacteria to enhance their internal carbon source storage capacity, thereby achieving the purpose of improving the carbon source storage performance of the activated sludge without increasing the carbon source demand in the influent.

The invention belongs to the technical field of microbial agents, and particularly relates to a microbial agent for increasing the content of glycogen in oysters. The microbial agent is obtained by coating bacillus, lactic acid bacteria and monad with sodium alginate, calcium chloride, calcium alginate and chitosan. According to the application, the number and activity of viable bacteria in the microbial agent are improved by adjusting the content of NaCl, the temperature and the pH value; according to the prepared microbial agent for increasing the glycogen content of the oysters, the functions of the used microorganisms are complementary, through the cooperation of immunity and metabolism, synthesis of the glycogen is increased, consumption of the glycogen is reduced, the synthesis efficiency of the glycogen in the oysters is enhanced, the glycogen content of the oysters is increased, and an innovative solution is provided for improving the quality and efficiency of oyster culture.