87 results about "Glycogen" patented technology

The invention provides a traditional Chinese medicine composition and application thereof in preparation of anti-fatigue health care wine, and relates to the technical field of traditional Chinese medicine preparation and traditional Chinese medicine health care. The traditional Chinese medicine composition takes medicinal and edible traditional Chinese medicinal materials as raw materials, is prepared from herba cistanche, radix acanthopanacis semticosi, radix asparagi, fructus phyllanthi, pollen typhae, fructus canarii, herba cichorii, folium microcotis, semen canavaliae and fructus citri, and has the effects of tonifying kidney and spleen, tonifying qi and yin and combining clearing and tonifying. The traditional Chinese medicine composition and the health-care wine prepared from the traditional Chinese medicine composition provided by the invention have an excellent anti-fatigue effect, and can be used for remarkably prolonging the weight-bearing exhaustion swimming time of mice, remarkably reducing the plasma urea nitrogen content of the mice after exercise, remarkably improving the hepatic glycogen content of the mice and remarkably improving the blood lactic acid clearance of the mice after exercise; the obvious physical fatigue relieving effect is realized. The compound is expected to be developed into anti-fatigue medicines or health-care products.

The invention provides a domestic sewage treatment method and system based on shortcut nitrification and anaerobic ammonia oxidation of Anammox bacteria preposed alkali reduction, and relates to the technical field of sewage treatment. The method comprises the following steps: anaerobic treatment: converting an organic suspended solid into an easily degradable carbon source through hydrolytic acidification bacteria; the carbon source is absorbed by denitrifying phosphorus-accumulating bacteria and denitrifying glycogen-accumulating bacteria and is stored as an intracellular carbon source; primary anoxic treatment: reacting ammonia nitrogen with nitrite through Anammox bacteria, and synchronously reducing alkalinity; aerobic treatment: under the condition of low alkalinity, in combination with low dissolved oxygen control, Comamox bacteria oxidize ammonia nitrogen into nitrite and accumulate the nitrite; and secondary anoxic treatment: denitrifying residual oxidation nitrogen into nitrogen by using intracellular carbon through denitrifying glycogen-accumulating bacteria. Through the synergistic effect of the hydrolytic acidification bacteria, the denitrifying phosphorus-accumulating bacteria, the Anammox bacteria, the Commox bacteria and the denitrifying glycogen-accumulating bacteria, efficient nitrogen and phosphorus removal is realized.

The present invention relates to a process for purifying phycocyanins from a solution comprising phycocyanins and glycogen, said phycocyanins being produced by microorganisms belonging to the order Cyanidiales, characterized in that it comprises (i) an enzymatic degradation step of glycogen with an enzyme suitable for degrading glycogen at a pH below 6 and a reaction temperature below 40°C, said enzyme being selected from a pectinase, a glucoamylase, or a mixture of a pectinase and a pullulanase, and (ii) a step of separating phycocyanins from the glycogen degradation products.

The invention belongs to the technical field of protein peptide preparation, and particularly relates to wheat oligopeptide with an anti-fatigue effect and a preparation method of the wheat oligopeptide. According to the method, the raw materials are subjected to enzymolysis by using the first compound enzyme containing bacillus subtilis protease and bromelain and the second compound enzyme containing trypsin, flavourzyme and papain step by step, and treatment of centrifugation, ultrafiltration and the like is combined, so that the yield and the taste of the product are improved, and the proportion of small molecular weight peptide in the product is high. Therefore, the wheat oligopeptide prepared by the method is high in yield and good in taste, not only is beneficial to improving the endurance of the body and prolonging the exercise time, but also can improve the energy metabolism condition in the body, delay the accumulation of lactic acid, urea nitrogen and creatine kinase in the body after exercise and increase the glycogen storage amount, and macroscopically shows certain anti-fatigue ability. The technical scheme of the invention has strong practicability, is beneficial to industrial production, and can be applied to preparation of anti-fatigue functional food.

This invention belongs to the field of heavy metal removal technology, specifically disclosing an extract from the leaves of a Solanaceae plant, its extraction method, and its application. The extraction method includes the following steps: S1, mixing tomato and pepper leaves, chopping them to obtain a preliminary mixture; S2, mixing a compound microbial agent with the preliminary mixture obtained in S1, fermenting, sterilizing, centrifuging, filtering through a 0.45μm filter membrane, discarding the precipitate, and obtaining a fermented preliminary mixture; S3, concentrating the fermented preliminary mixture obtained in S2 under reduced pressure to obtain a concentrated fermentation broth; S4, adding sodium carboxymethyl cellulose to the concentrated fermentation broth obtained in S3, stirring evenly, and freeze-drying to obtain the Solanaceae plant leaf extract. This Solanaceae plant leaf extract can effectively remove cadmium from aquatic products, aiding in the growth recovery of aquatic products after cadmium stress, and can also effectively maintain the pH value of white clam muscle, reducing lactic acid accumulation and glycogen consumption, and maintaining the freshness of the meat.

The invention discloses a camelina sativa seed meal oligosaccharide extract capable of regulating blood sugar and a preparation method of the camelina sativa seed meal oligosaccharide extract, and the camelina sativa seed meal oligosaccharide extract is extracted from camelina sativa seed meal through fermentation, heating extraction, cooling alcohol precipitation and enzymolysis. Comprising a camelina seed meal oligosaccharide extract and a cortex fraxini iridoid component extract. The cortex fraxini iridoid component extract is obtained through microwave-assisted enzymolysis extraction and purification; the invention further discloses application of the blood sugar reducing composition in blood sugar reducing food, medicine or health care products. The camelina seed meal oligosaccharide extract, the cortex fraxini iridoid component extract and the hypoglycemic composition have inhibition ability on alpha-glucosidase and alpha-amylase, can improve glucose consumption, glycogen content and glycolysis key enzyme activity, effectively improve insulin resistance, improve oxidative stress, regulate intestinal flora structure, and improve the blood sugar level, so that the hypoglycemic effect of the composition is improved. The traditional Chinese medicine is safe and non-toxic and has a good effect on treating diabetes and complications.

The application discloses a kind of immortalization human umbilical cord mesenchymal stem cell NOTCH1-LNR overexpression stable cell line construction method and its application in liver differentiation, it is related to immortalized cell technical field.The application first connects target gene to vector, and the slow virus plasmid containing target gene is constructed, the slow virus plasmid containing target gene is introduced into 293T cell, and high titer slow virus containing target gene is generated;Immortalized human umbilical cord mesenchymal stem cell is infected with slow virus, and mixed clone cell strain is screened, and the immortalized human umbilical cord mesenchymal stem cell NOTCH1-LNR overexpression stable cell line is obtained.The application proves that the differentiation efficiency of immortalized human umbilical cord mesenchymal stem cell NOTCH1-LNR overexpression can effectively improve liver-like cell to glycogen staining and Western blotting experiment.This research result is expected to provide new technical thought for the next step development to improve the yield of MSCs liver differentiation.

The invention provides a respiratory-gating-free free-breathing liver glycogen quantitative magnetic resonance imaging method and system, and the method comprises the steps: applying a chemical exchange saturation transfer imaging sequence to a liver region of a subject, and the imaging sequence comprises saturation pulses of a plurality of saturation frequency deviation points; a radial blade type k-space acquisition strategy is adopted to acquire magnetic resonance signal data, the radial blade type k-space acquisition strategy comprises a plurality of blades, each blade is composed of a group of parallel k-space lines, and different blades are rotationally distributed by taking the k-space center as a rotating shaft; performing image reconstruction on the acquired k space data to obtain magnetic resonance images under different saturation frequency deviations; and performing fitting analysis on the magnetic resonance image by adopting a multi-pool Lorentz model to obtain quantitative information of the liver glycogen. According to the method, the interference of respiratory movement on the CESTMRI data acquisition process can be eliminated, so that the accurate quantification of the liver glycogen in the free respiration state is realized.

Provided herein is a noninvasive method for measuring pH, glucose and glycogen levels using a single acquisition method, as well as systems for performing the method. This method for glycogen / glucose (and pH) detection has high specificity enabled by superior chemical shift separation in high-strength magnetic fields. The methods have applications in detecting changing tumor microenvironments in cancerous cells for measuring important metrics for noninvasive and quantitative metabolic profiling.

The invention relates to the technical field of biological treatment of urban domestic sewage, in particular to a stable deep denitrification method for aerobic granular sludge based on a reactor, which is characterized in that the reactor is operated by adopting a gradient water inlet mode combined with a dissolved oxygen regulation and control strategy, that is, short-time rapid water inlet is applied before slow water inlet to create an unbalanced external carbon source supply condition; internal carbon source storage and enrichment of slow-growing microorganisms such as phosphorus-accumulating bacteria and glycogen-accumulating bacteria are promoted, so that the stability of the particle structure is enhanced; the dissolved oxygen of the reactor main body is gradually reduced to 2 mg / L so as to reduce the inward permeation of the dissolved oxygen, increase the internal anaerobic zone and improve the denitrification rate, and meanwhile, a transient high oxygen exposure condition is set at the tail end of the aerobic stage so as to form high hydraulic shear force to further maintain the stability of the particles. The structure regularity, the mechanical strength and the denitrification performance of the aerobic granular sludge are remarkably improved, the average removal rate of ammonia nitrogen and the average removal rate of total nitrogen are stabilized at 97.84% and 88.87% or above respectively, the synchronous nitrification endogenous denitrification efficiency reaches 92.10% or above, and long-term stable operation of the system is guaranteed.

The present invention relates to a treatment method for degrading glycogens present in biomass of unicellular red algae (URA), in particular of the genus Galdiella, and to the products thus obtained.

The invention relates to the technical field of microbial fertilizer preparation, and provides a coating method for improving the coating rate of microbial fertilizer particles in order to solve the problem of poor affinity between a hydrophilic material and hydrophobic microbial fertilizer particles and improve the coating rate of the hydrophilic material as a film layer to the microbial fertilizer particles. The fertilizer is composed of a microbial fertilizer particle core layer and a modified film layer coating the outer layer of the fertilizer, and the mass ratio of the core layer to the film layer is 100: (0.5-10). Wherein the core layer can be made of trichoderma harzianum, penicillium baylii or aspergillus niger microbial fertilizer particles; the raw material of the film layer can be composed of the following components: polyvinyl alcohol (PVA), a hydrophilic material (such as starch, glucose, maltose or dextrin) capable of providing glycogen, a surfactant capable of changing the surface tension of water, a wetting agent, a dispersing agent (such as lauryl sodium sulfate, disodium methylene dinaphthalene sulfonate, sodium dodecyl benzene sulfonate or fatty acid salt) and the like. According to the coating method for improving the coating rate of the microbial fertilizer particles, the higher coating rate is achieved, the coated microbial fertilizer further has the longer container life and the higher pressure resistance, the looseness problem is greatly solved, the problems that traditional microbial fertilizer particles are prone to inactivation, breaking and loosening are solved, and the yield of the microbial fertilizer particles is improved. Good popularization and application values are realized.

Use of dimethyl sulfoxide (DMSO) or glycofurol or their combination as an additive in a solution comprising a solvent and at least one active ingredient selected from melatonin and / or an antioxidant metabolite, derivative or analogue thereof, for prolonging stability and / or shelf life of said at least one active ingredient in said solution by retarding a decomposition of said at least one active ingredient comprised in said solution.

The present application relates to the technical field of biotechnology, and particularly relates to a tagatose-6-phosphate phosphatase mutant and a method for synthesizing tagatose from scratch. The method provided by the present application is a one-pot method for biosynthesizing D-tagatose by using starch or dextrin as a substrate, adopting a multi-enzyme cascade reaction system composed of a tagatose-6-phosphate phosphatase mutant, a glycogen-debranching enzyme, an alpha-glucan phosphorylase, a phosphoglucomutase, a glucose-6-phosphate isomerase, a D-tagatose-6-phosphate-3-epimerase and a 4-alpha-glucan transferase. The method can prepare tagatose from ordinary and inexpensive starting materials (starch or dextrin) at a high yield, improves the overall conversion rate of the system and the final yield of tagatose, provides a new technical approach for realizing low-cost and green production of tagatose, and has the advantages of high yield, low cost and being more suitable for industrial production.

This disclosure relates to the use of glycogen phosphatase B agonists in the treatment of neurodegenerative diseases. Specifically, this disclosure provides the use of glycogen phosphatase B agonists (preferably adenosine monophosphate) in the preparation of medicaments for the prevention or treatment of neurodegenerative diseases. This disclosure demonstrates that glycogen phosphatase B agonists (AMPs) hold promise as a safe and effective treatment option for patients with neurodegenerative diseases.

The invention discloses an application of miR-146b-3p inhibitor in improving sheep embryo implantation abnormity caused by LPS (Lipopolysaccharide), and belongs to the technical field of biology, and the sequence of the miR-146b-3p inhibitor is shown as SEQ ID No.1. The invention further discloses a preparation method of the miR-146b-3p inhibitor. LPS can cause sheep endometrial epithelial cell inflammatory factor abnormity, adhesion molecule expression change, trophoblast sphere adhesion growth inhibition and miR-146b-3p expression increase, miR-146b-3p inhibits GYS2 gene expression in a targeted manner, endometrial glycogen synthesis is reduced, and embryo implantation is affected. The miR-146b-3p inhibitor can be used for rescuing GYS2 expression reduction caused by LPS (Lipopolysaccharide), relieving glycogen synthesis reduction and expression inhibition of an attachment key gene, promoting sheep embryo attachment and reducing the embryo loss rate. The invention further provides a reagent containing the inhibitor, a reagent for overexpressing the GYS2 gene, a method for promoting embryo implantation and related products, a new direction is provided for improving the reproductive performance of sheep, and the inhibitor has important value and significance for the sheep raising industry.

The invention relates to the technical field of food science and biology, and discloses a multi-element plant active peptide with a synergistic anti-fatigue effect as well as a preparation method and application of the multi-element plant active peptide. According to the preparation method, soybean protein, wheat protein and pollen pini in a specific mass ratio are used as raw materials, and enzymolysis treatment is performed by adopting a composite enzymolysis technology in combination with multi-frequency pulse ultrasound assistance. The multi-element plant active peptide prepared by the method has a specific molecular weight distribution range (600-1500Da), and the branched chain amino acid content of the multi-element plant active peptide is high. Mouse experiments show that the active peptide can significantly improve the endurance, muscle glycogen reserve and liver SOD activity of mice, and the synergistic anti-fatigue effect of the active peptide is verified.

The invention relates to application of theaflavin as a UGP2 inhibitor and application of theaflavin in preparation of a medicine for preventing and / or treating hepatocellular carcinoma, and belongs to the technical field of biology. According to the invention, the monomeric compound theaflavin derived from a natural product can be used as a UGP2 direct targeting inhibitor for the first time, and is used for preparing a liver cancer treatment medicine for regulating and controlling glucose metabolism reprogramming. The theaflavin can be specifically and directly combined at a UGP2 active site in a targeting manner through high activity to inhibit the activity of the UGP2, is the first specific inhibitor which takes the UGP2 as a target spot and is reported at present, and can be used for remarkably inhibiting tumor phenotypes such as proliferation, migration and invasion of liver cancer cells by inhibiting UGP2-mediated UDPG synthesis and intracellular glycogen accumulation.

The invention belongs to the technical field of microbial agents, and particularly relates to a microbial agent for increasing the content of glycogen in oysters. The microbial agent is obtained by coating bacillus, lactic acid bacteria and monad with sodium alginate, calcium chloride, calcium alginate and chitosan. According to the application, the number and activity of viable bacteria in the microbial agent are improved by adjusting the content of NaCl, the temperature and the pH value; according to the prepared microbial agent for increasing the glycogen content of the oysters, the functions of the used microorganisms are complementary, through the cooperation of immunity and metabolism, synthesis of the glycogen is increased, consumption of the glycogen is reduced, the synthesis efficiency of the glycogen in the oysters is enhanced, the glycogen content of the oysters is increased, and an innovative solution is provided for improving the quality and efficiency of oyster culture.

Recombinant AAV (rAAV) vectors comprising a rAVV genome comprising a heterologous nucleic acid encoding a lysosomal protein, e.g., acid alpha-glucosidase (GAA) polypeptide, and optionally a signal peptide and / or optionally a targeting sequence, e.g., IGF2 targeting peptide, operatively linked to a liver-specific promoter (LSP), enabling the GAA polypeptide to be secreted from the liver and targeted to the lysosomes. Particular embodiments relate to a recombinant AAV (rAAV) vector encoding an alpha-glucosidase (GAA) polypeptide, having a liver secretory signal peptide and a IGF2 targeting peptidethat binds human cation-independent mannose-6-phosphate receptor (CI-MPR) or to the IGF2 receptor, permitting proper subcellular localization of the GAA polypeptide to lysosomes. Also encompassed are cells, and methods to treat a lysosomal disease, for example, a glycogen storage disease type II (GSD II) disease and / or Pompe Disease with the rAAV vector.

The application discloses a recombinant humanized collagen composition for resisting HPV infection and a preparation method thereof. The composition comprises the following components in percentage by mass: 30-45% of a recombinant humanized collagen fusion protein, 30-40% of probiotics and 20-35% of glycogen. The recombinant humanized collagen fusion protein comprises, from N-terminal to C-terminal, a recombinant humanized collagen fragment and a human fibronectin fragment connected in sequence. The recombinant humanized collagen fragment and the human fibronectin fragment are connected by a linker. The recombinant humanized collagen fusion protein in the composition can promote the adhesion and colonization of the probiotic composition to the vaginal epithelial cells, rapidly improve the microbial environment of the vagina and effectively enhance the resistance of the vagina to harmful bacteria and viruses.

The invention discloses a plant beverage containing birch juice and lime juice and a processing technology of the plant beverage. The plant beverage comprises the following raw materials in percentage: 10%-15% of apple juice, 5%-10% of lime juice, 0.5%-1% of white sugar, 1%-3% of xylitol, 5%-7% of green tea, 20%-25% of birch juice, 2%-5% of lucid ganoderma, 1%-3% of ginseng, 3%-7% of pericarpium citri reticulatae, 0.15%-0.25% of fructo-oligosaccharide, 0.1%-0.5% of an elderberry extract and 0.2%-0.6% of acerola cherry powder. And the balance of drinking water. The processing technology comprises the following steps: S1, raw material pretreatment: collecting birch juice, juicing lime, and extracting lucid ganoderma and ginseng to prepare a lucid ganoderma and ginseng mixed solution; the elderberry anthocyanin and the birch juice polysaccharide block virus adsorption, the ganoderan and the ginsenoside improve the activity of NK cells, the birch juice regulates synthesis of hepatic glycogen, the fructo-oligosaccharide promotes generation of short-chain fatty acid, the lime juice and the acerola cherry powder VC form a redox cycle, and the effect of improving the immunity is achieved.

The invention relates to the technical field of preparation of probiotic fermented beverages, in particular to a probiotic fermented fruit and vegetable beverage with functions of resisting fatigue and enhancing immunity. The hawthorn fruit beverage specifically comprises the following components in parts by mass: 60-80 parts of Zhongtian big hawthorn fruits, 10-20 parts of autumn pears, 11-15 parts of grapes, 9-15 parts of kiwi fruits, 6-10 parts of pomegranates, 3-5 parts of figs, 3-5 parts of beet roots, 3-5 parts of pumpkins, 1-2 parts of kale, 1-2 parts of honey and 2-3 parts of zymophyte. The beverage product provided by the invention can significantly prolong exhaustive swimming time, improve liver glycogen reserve, reduce serum urea nitrogen level after exercises, and effectively relieve physical fatigue; meanwhile, the food can comprehensively enhance the immune function of the organism, including improving the activity of phagocytes, promoting the proliferation of lymphocytes and enhancing humoral immune response, and provides a natural and healthy solution for resisting fatigue and enhancing the immunity function through daily diet for consumers.

Adult form glycogen storage disease type III (GSD III) is an orphan neuromuscular disorder caused by a deficiency of glycogen debranching enzyme. Long-term complications include progressive liver fibrosis, hepatic failure, and end-stage liver cirrhosis, and progressive muscle myopathy. Presently, there are no clinically approved therapies or cures for GSD III. Disclosed herein are compositions for and methods of treating and / or preventing GSD III disease progression.

The invention discloses a deep nitrogen and phosphorus removal method for directionally regulating and controlling phosphorus-accumulating bacteria and glycogen-accumulating bacteria in allusion to low-concentration municipal sewage. In a sequencing batch reactor, low-concentration municipal sewage is used as a matrix, and coupling of PAOs / DPAOs and GAOs / DGAOs in aerobic granular sludge is fully utilized to realize deep nitrogen and phosphorus removal of wastewater. In the starting stage, sludge is regularly discharged through an A / O operation mode to rapidly enrich PAOs; in the operation stage, through an A / O / A mode, sludge age control and dissolved oxygen concentration regulation, DGAOs enrichment is realized, conditions are created for DPAOs appearance, NOB is inhibited, and short-cut nitrification endogenous denitrification is promoted. According to the method, the abundance balance of PAOs / DPAOs and GAOs / DGAOs is achieved by adopting a periodic carbon source multiplication coupling PO4 < 3-> concentration control technology and post-anoxic section time length regulation and control in the stable period of the system, at the moment, yellow particles and white particles in the system coexist, the cultured aerobic particles are stable in structure, and synchronous carbon, nitrogen and phosphorus synergistic removal can be achieved. According to the method for directionally regulating and controlling PAOs / DPAOs and GAOs / DGAOs provided by the invention, the removal rate of COD (Chemical Oxygen Demand) in sewage can be more than 90%, and the removal rate of TN (Total Nitrogen) / TP (Total Protein) is nearly 100%.

A microbiome modulating composition and a method for producing the microbiome modulating composition are described herein. The method comprises preparing a culture from at least two of any Lactobacillus spp. bacteria, Limosilactobacillus spp. bacteria, or combinations thereof, passaging the culture on a glycogen carbon source to form a postbiotic ferment of the at least two of any Lactobacillus spp. bacteria, Limosilactobacillus spp. bacteria, or combinations thereof, and adding the postbiotic ferment to a carrier. Further disclosed is a method for treating microbial dysbiosis of an urogenital area or a skin area of a user, the method comprising applying the microbiome modulating composition to an urogenital or a skin area of a user. The disclosed methods and microbiome modulating compositions help promote the growth of healthy Lactobacillus spp. bacteria to prevent dysbiosis and other related conditions and infections.

This invention discloses a method for DNA extraction from edible oils. The method includes: weighing 10g of oil or phospholipid into a first centrifuge tube, adding 15ml of n-hexane and 2ml of EFG buffer, vortexing and centrifuging, and collecting 1ml of the bottom liquid after separation into a second centrifuge tube; adding 500μl of chloroform to the second centrifuge tube, inverting to mix, and centrifuging again, collecting the supernatant into a third centrifuge tube; adding 0.8 times the volume of isopropanol and 4μl of glycogen to the third centrifuge tube, mixing, and allowing to stand at room temperature for at least 30 minutes, centrifuging again, discarding the supernatant, adding 500μl of 75% ethanol, centrifuging again, discarding the supernatant, drying at 65°C for 5 minutes, adding 60μl of TE buffer to dissolve the precipitate, placing at 65°C for 2 minutes, and storing the DNA at -20°C for later use. This invention saves time and costs, yields highly stable DNA, has relatively low environmental toxicity, is safe and reliable, uses fewer consumables, and is inexpensive.

The invention relates to the technical field of bioactive peptides, and discloses a directional preparation method of oyster flavor peptides, which comprises the following steps: (1) microfluidic high-pressure shearing; (2) ultrahigh pressure assisted glycogen decomposition; (3) synergistic enzymolysis; (4) peptomics analysis; (5) carrying out batch molecular docking on the oyster peptide and a T1R1 / T1R3 taste receptor; (6) sonochemical synergistic ultrafiltration separation; and (7) performing microwave-assisted freeze drying to obtain oyster umami peptide powder. A microfluidic high-pressure shearing technology is combined with an ultrahigh-pressure glycogen pre-decomposition technology, so that the utilization efficiency of raw materials is remarkably improved; fluid mechanical force generated by microfluidic high-pressure shearing can mildly regulate and control conformation of oyster protein molecules, and the solubility and enzymolysis accessibility of the oyster protein molecules are improved; the compact structure of glycogen particles is destroyed through ultrahigh pressure treatment, directional removal of glycogen is achieved in combination with a special enzyme system, the problem of high glycogen interference peculiar to oysters is effectively solved, and a foundation is laid for follow-up efficient enzymolysis.

The invention provides a bacillus amyloliquefaciens strain BLBymyt-03, a production method of isomaltulose and application of the bacillus amyloliquefaciens strain BLBymyt-03, and the preservation number of the bacillus amyloliquefaciens strain BLBymyt-03 is CGMCC No.36184, and the preservation date is October 15, 2025. The strain can be used for preparing the sucrose isomerase through fermentation, so that the enzyme activity of the sucrose isomerase reaches 1252 U / mL. The strain can also be used for preparing isomaltulose and achieving conversion of high-concentration glycogen, the content of the converted isomaltulose is larger than 93%, the content of seaweed kelose is smaller than 5%, the substrate conversion rate reaches 92% or above, the primary crystallization purity is larger than 99%, and the yield is larger than 85%. Meanwhile, in crystals obtained through primary crystallization, the proportion of 30-80-mesh products reaches 80% or above, and the particles are uniform and good in fluidity.

Described herein are kits and methods for quantifying glycogen in at least one sample. Disclosed methods involve detecting a presence of linear glucose chains in at least one sample and plurality of pre-applied glycogen standard spots; optionally generating a calibration curve based on the detection of linear glucose chains from the pre-applied glycogen standard spots; and quantifying an amount of glycogen in the at least one sample based on amounts of the linear glucose chains detected in the at least one sample and the pre-applied glycogen standard spots.