15 results about "Islet" patented technology

The disclosure generally relates to compositions and methods for the treatment of cancer. In some aspects, disclosed herein are methods for the induction of synthetic lethality with epigenetic therapy (ISLET) using a combination of at least one epigenetic compound and at least one chemotherapeutic agent. Also disclosed herein are screening methods for identifying compounds that induce killing of cancer cells when combined with at least one epigenetic compound. Further disclosed herein are methods of potentiating a therapeutic effect of a chemotherapeutic agent against a cancer, comprising administering to a subject having the cancer an epigenetic compound in an amount effective to potentiate the therapeutic effect of the chemotherapeutic agent against the cancer.

The utility model discloses an adjustable skin stitching instrument which comprises an attachment plate, two symmetrically-arranged mounting plates are arranged on one side of the attachment plate, and the first mode is that the mounting plate on one side is provided with a plurality of single-body lock catches or double-body lock catches, and the mounting plate on the other side is provided with a plurality of double-body bases or single-body bases; according to the second mode, the mounting plates on the two sides are provided with a plurality of single lock catches or double-body lock catches and the double-body base or the single base in a crossed mode; the base is connected with a pull rod, a plurality of locking rings are arranged on the side wall of the pull rod, a cavity is formed in the lock catch, and a locking block is arranged in the cavity. According to the coral island lagoon face traction device, the combined structure of the double-body lock catch or the single-body lock catch, the single-body base or the double-body base, the mounting plate, the pull rod and the like is arranged, so that a coral island reef'ring reef lagoon face '-shaped combined traction range can be formed at the wound of a patient, and a U-shaped traction range is formed between the double-body lock catch or the double-body base; stress can be reduced during transverse closing and longitudinal extending of the incision, and healing of the incision is promoted.

The present invention discloses a method for detecting the activity of islet cells derived from human pluripotent stem cells: the islet cell spheres to be detected are digested to obtain a single-cell suspension; 0.1-5% HSA by volume is added to the PBS buffer as a cell protection solution, the cell protection solution is added to the single-cell suspension, and then PI staining solution is added for staining; the stained sample is subjected to flow cytometry to obtain the proportion data of live cells. The present invention uses a low-concentration PI staining solution for staining to improve the staining effect. The stained cells are sorted by flow cytometry. HSA is used as a cell protection solution to protect the islet cells during the flow cytometry detection, reduce the sample preparation time and the damage to islet cells caused by flow cytometry detection, maintain cell activity, can be standardized and normalized, avoid human judgment errors, objectively and accurately detect the islet viability, has high repeatability, and has a low detection cost, and is expected to be applied to the determination of viability before clinical islet transplantation.

The invention relates to the technical field of preparation of traditional Chinese medicine preparations, in particular to a preparation method of a herbaceous plant for conditioning diabetes mellitus, and the herbaceous plant is prepared from 200g of folium mori, 300g of bitter gourd, 150g of black fungus, 150g of radix angelicae pubescentis, 150g of radix paeoniae rubra, 300g of radix astragali, 200g of rhizoma atractylodis macrocephalae, 200g of poria cocos, 60g of ginseng, 200g of radix salviae miltiorrhizae, 200g of fructus gardeniae, 150g of American ginseng and 120g of radix notoginseng. Effective components of all plants can be reserved to the maximum extent, meanwhile, various medicinal material components are mutually compatible, radix astragali has the effects of tonifying qi and lifting yang, rhizoma atractylodis macrocephalae has the effects of tonifying spleen and eliminating dampness, poria cocos has the effects of clearing damp and promoting diuresis and the like, and tonifying products such as ginseng and American ginseng are matched and have a synergistic effect with blood glucose reducing components such as bitter gourd and folium mori; the diabetes is comprehensively conditioned from multiple aspects of regulating body metabolism, enhancing immunity, improving pancreas islet functions and the like, and the overall drug effect is improved.

The invention relates to a microneedle platform that enables rapid and safe immune reaction assessment by single application of allergens contained in microneedle-containing islets (2) positioned at certain intervals on a biocompatible material developed for use in allergy skin tests. Unlike the methods used in the art, the inventive microneedle platform, which enables the simultaneous application of different allergens, also enables immune cells in different layers of the skin to react against allergens with microneedles of varying sizes within the unit microneedle area.

Hydrodynamic methods for conformally coating non -uniform size cells and cell clusters for implantation, thus preventing immune rejection or inflammation or autoimmune destruction while preserving cell functionality. A method for conformally coating cells and c clusters with hydrogels that are biocompatible, mechanically and chemically stable and porous, with an appropriate pore cut-off size. The methods of the invention are advantageously reproducible and result in a relatively high yield of coated versus non-coated cell clusters, without compromising cell functionality. Conformal coating devices configured to perform the methods of the invention, methods of optimally utilizing said devices and purifying the coated islets, and coated biomaterials made by said methods.

The invention discloses a blood island organoid polar structure culture device, a blood island organoid culture device and a culture method thereof, and relates to the technical field of stem cell and organoid engineering. According to the method, the problems that stable, directional and controllable fluid shear force cannot be provided, and the organ cannot be accurately positioned, so that the stress of the organ is non-uniform, and a physiological correlation polar structure is difficult to form in an existing blood island organ in-vitro culture technology are solved. According to the device disclosed by the invention, the magnetic rotor rotates to generate a directional vortex in the hole, and a double-screen structure is combined to carry out accurate positioning and physical isolation on blood island organs, so that continuous, stable, consistent-direction and controllable-strength fluid shearing force is provided for the blood island organs. The in-vivo blood island development mechanical microenvironment is effectively simulated. The blood island organ can be efficiently induced to form a polar structure and the hematopoietic function maturation is promoted. The system is compatible with a standard porous plate system, supports high-throughput culture, and provides an advanced platform for blood island development mechanism research and drug screening.

Compounds comprising a rhodanine scaffold, compositions comprising same, method of using such compounds find compositions to inhibit tubulin-associated unit (tau) protein aggregation or alpha-synuclein (α-syn) protein aggregation in a subject having, or at risk for, tau protein aggregation or α-syn protein aggregation, respectively; and methods of using such compounds and compositions to inhibit islet amyloid polypeptide (LAPP) fibril formation and to detect LAPP fibrils.

Type 1 diabetes mellitus (T1D) is caused by autoimmune disruption of insulin-producing beta cells in the Langerhans island, resulting in survival-dependent exogenous insulin injection. There is a need for treatment that can prevent or delay clinical T1D onset in high risk individuals. A promising therapy is an anti-CD3 monoclonal antibody tollizumab, since several studies have shown that short-term treatments permanently reduce loss of beta cell function, the effect also being observed 7 years after diagnosis and treatment. In addition, an improved method for predicting such prevention or delay is also required. In one aspect, provided herein is a method of predicting the reactivity of an anti-CD3 antibody in preventing or delaying T1D onset.

The present invention provides synthetic peptides, pharmaceutical compositions containing synthetic peptides, and the use of synthetic peptides for the treatment of amyloidosis. [Solution] The synthetic peptide of the present invention has the effect of preventing the formation of β-amyloid aggregation and preventing the folding and aggregation of β-amyloid (amyloidβ, Aβ), tau protein, α-synuclein (α-synuclein, α-Syn), islet amyloid polypeptide (IAPP), or transthyretin (TTR), thereby treating amyloidosis.

The disclosed concept includes the preparation and use of biocompatible bioinks, as well as techniques and methods therein for bioprinting, preparing and 3D printing primary cells and patient-specific cells and forming functional soft tissue in a three-dimensional construct form that incorporates the cells and mimics native tissues. The biocompatible bioinks include alginate, methylcellulose, and primary or iPSC-derived cells in the form of single cells or aggregates. The bioprinted 3D construct includes one or more of the iPSC organoids, human islets, as well as TEPCs.

The utility model discloses an isatis root seedling comparison device for test, which comprises a cultivation tray, a plurality of cultivation grooves are uniformly arranged on the cultivation tray, two convex grooves are symmetrically arranged on the cultivation tray, convex seats are arranged in the two convex grooves in a sliding manner, the side ends of the two convex seats are fixedly connected with fixing frames, and the fixing frames are fixedly connected with the fixed frames. First sliding plates are slidably arranged in the two fixing frames, first measuring plates are fixedly connected to the upper sides of the middles of the two first sliding plates, first scale marks are arranged at the side ends of the first sliding plates, first screws matched with the first sliding plates are in threaded connection with the fixing frames, and sliding grooves are formed in the top ends of the two first sliding plates. Second sliding plates are arranged in the two sliding grooves in a sliding mode, second measuring plates are fixedly connected to the upper sides of the middles of the two second sliding plates, and isatis root seedlings within the corresponding height range can be accurately selected out conveniently and subjected to a culture experiment.

Provided herein are endocrine progenitor cells and organoids derived from adult islets in vitro, as well as methods for making and using the same. The compositions are useful for treating or preventing diabetes, other diseases or disorders characterized by impaired islet function, and symptoms thereof.