562 results about "Lysozyme" patented technology

The invention relates to the technical field of hydrogel, in particular to a preparation method and application of EGCG-mediated hydrogel, a lysozyme solution is heated under the acidic condition to form lysozyme amyloid fibrils LAFs, EGCG is dissolved in a buffer solution and mixed with the LAFs solution obtained in the step 1 in proportion to form an LAFs-EGCG compound, and the EGCG-mediated hydrogel is prepared. The chitosan CS is dissolved in an acetic acid buffer solution, glutaraldehyde is added to serve as a cross-linking agent, the mixture is mixed with the LAFs-EGCG compound obtained in the step 2 and stirred, the composite hydrogel is formed, the concentration of EGCG is 0.2-1.0 wt%, the concentration of LAFs is 5 wt%, the concentration of chitosan is 2 wt%, the pH value of the lysozyme solution in the step 1 is 2.0, the heating temperature is 77 DEG C, the heating time is 10 hours, the concentration of EGCG in the step 2 is 0.4-0.8 wt%, and the pH value of the lysozyme solution in the step 2 is 1.5-2.5. In the step 3, the concentration of glutaraldehyde is 2%, the storage modulus G'of the hydrogel is 325-480 Pa, the hardness is 2176.71 g, and the bacteriostasis rate on staphylococcus aureus and salmonella exceeds 95%.

The invention provides a kit for rapidly detecting MRSA and drug-resistant gene nucleic acid POCT of the MRSA based on a phage lyase mutant. The kit comprises nucleic acid extraction and purification and triple PCR rapid amplification detection reagents. The staphylococcus aureus bacteriophage lyase mutant nucleic acid release liquid containing the amino acid sequence as shown in SEQ ID NO: 1 is adopted, wall breaking can be rapidly conducted on staphylococcus aureus, and nucleic acid is released. The effect of the nucleic acid release liquid acting for 5 minutes is equivalent to or even better than that of the lysozyme acting for 60 minutes; according to the present invention, with the combination of the MRSA and drug-resistant gene triple PCR rapid amplification detection reagent, the total detection time is only 35 min, the time is saved by 55 min or more than 55 min compared with the lysozyme method, and the MRSA and drug-resistant gene nucleic acid POCT rapid detection can be achieved;

The invention provides an additive composition containing a bioactive enzyme and a preparation method of the additive composition, and belongs to the technical field of biological enzymes. The preparation method comprises the following steps: fixing phytic acid and cyclodextrin on hydroxyapatite, modifying with tannic acid, reacting with dithiothreitol modified lysozyme to prepare an immobilized compound, adding 2, 2-dimethoxy-2-phenylacetophenone and methyl methacrylate, and carrying out irradiation reaction with an ultraviolet lamp to prepare the additive composition containing the bioactive enzyme. The broad-spectrum antibacterial property and the antiviral property of the lysozyme are greatly improved, the sterilization effect on gram negative bacteria is obviously improved, meanwhile, the stability of enzyme is greatly improved, the enzyme is not prone to inactivation, the preservation time of the enzyme is prolonged, the application effect of the enzyme is widened, meanwhile, the lysozyme has good killing capacity on oral bacteria and viruses, the addition of preservatives is reduced, and the cost is reduced. The cleaning effect and stability of the product are improved, tooth demineralization can be prevented, decayed teeth can be prevented, and wide application prospects are achieved.

The invention discloses lysozyme, antibacterial peptide derived from the lysozyme and application of the lysozyme in preparation of antibacterial drugs. According to the invention, 1, 640 protein sequences recorded in a ruminant rumen caterpillars genome database are systematically predicted based on a lysozyme prediction model, and finally, the lysozyme with the amino acid sequence as shown in SEQ ID No.1 is relatively strong in antibacterial activity and stable in antibacterial activity, and is slightly influenced by environmental factors. According to the invention, an antibacterial peptide region of the lysozyme is analyzed and determined, three peptide fragments with clear antibacterial activity are identified, and the amino acid sequences of the peptide fragments are respectively shown as SEQ ID No.2, SEQ ID No.3 or SEQ ID No.4. The antibacterial peptide provided by the invention has relatively strong membrane destroying ability and broad-spectrum antibacterial activity, and compared with integral protein, the antibacterial peptide is simpler in structure and more flexible in synthesis and modification, and is easier to break through a bacterial drug-resistant mechanism.

The present invention relates to a pet sterilizer and deodorizer, which belongs to the technical field of pet deodorization. The present invention promotes the long-term expression of hop extract and enhances the sterilization and deodorization effect through the synergistic effect of hop extract, persimmon tannin, and lysozyme. Furthermore, the composition is stable, natural, and pollution-free, and can be used in the oral cavity and in vitro of animals. The present invention also provides zinc ricinoleate and an antibacterial agent. Through the three-phase interaction of zinc ricinoleate, hop extract, and antibacterial agent, the overall stability is enhanced and the antibacterial time of the complex is prolonged while ensuring antibacterial and deodorizing effects. The present invention also provides a modified quaternary ammonium salt antibacterial liquid. The modified quaternary ammonium salt has enhanced stability and, when present together with zinc ricinoleate, promotes an enhanced deodorizing effect. The present invention also provides an Artemisia annua extract, a Gentiana root extract, and an Artemisia argyi extract, which can eliminate odor sources and significantly inhibit bacterial growth, thereby having significant antibacterial, deodorizing, and odor-removing effects.

The invention discloses a method for extracting PN / PDRN from lactobacillus bulgaricus and application of the PN / PDRN in relieving and anti-inflammatory aspects, belongs to the technical field of biochemistry, and solves the problems that in the prior art, the acquisition source of PN / PDRN is unstable, and pollution easily exists. According to the preparation method, cell walls of lactobacillus bulgaricus are preliminarily dissolved through alkaline conditions and lysozyme in the preparation process, cell membranes of bacteria are destroyed through lauryl sodium sulfate to be split, the conditions are mild, meanwhile, PVP is added to protect a PN structure, and the purity of the PN / PDRN is effectively improved through a final product prepared through the preparation method.

The invention provides an automobile lining material with sterilization and peculiar smell removal functions and a preparation method of the automobile lining material. The material for the automobile lining comprises a surface layer, a functional layer and a bottom layer which are sequentially arranged, wherein the functional layer is obtained by performing surface treatment on a fabric through an MOF-lysozyme material. Silver-based ZIF is formed by silver ions and dimethylimidazole, the characteristics of large specific surface area, adjustable aperture and high crystallinity of a traditional MOF material are reserved, and the silver-based ZIF has a certain antibacterial effect due to the silver ions, so that the antibacterial characteristic of a functional layer is enhanced; as the silver-based ZIF has the characteristics of large specific surface area and large aperture, more lysozyme can be covalently grafted, and the silver-based ZIF still has excellent broad-spectrum antibacterial performance; the silver-based ZIF material and the lysozyme synergistically enhance the bactericidal ability of the functional layer, so that the effects of long-acting sterilization, good antibacterial effect and peculiar smell elimination are achieved, the air quality in a vehicle is further improved, and a healthier and fresher riding environment is provided for passengers.

The invention discloses a calcium alginate-gelatin-lysozyme composite antibacterial film and a preparation method and application thereof, and relates to the technical field of food antibacterial packaging.The method comprises the steps that sodium alginate is dissolved in water to obtain a solution containing 1%-3% (w / w) of sodium alginate, 0.5%-3% (w / w) of gelatin is added, the mixture is heated to 50-85 DEG C in a water bath till the solution is in a dissolved state, and the solution is cooled to the room temperature to obtain the calcium alginate-gelatin-lysozyme composite antibacterial film. Finally, glycerol with the total mass fraction being 0.2%-0.5% is added to serve as a plasticizer, and a sodium alginate-gelatin composite film is prepared through drying with a solution casting method; placing the sodium alginate-gelatin composite membrane in a 0.5%-3% (w / w) CaCl2 solution to prepare a calcium alginate-gelatin composite membrane; placing the calcium alginate-gelatin composite membrane in a lysozyme solution, oscillating, taking out, and drying at 25-50 DEG C to obtain the calcium alginate-gelatin composite membrane. The method has the beneficial effects that the method is simple and easy to implement, has a good antibacterial effect and better mechanical properties, is high in safety, is green and environment-friendly, and can inhibit growth and reproduction of common pathogenic bacteria and putrefying bacteria.

This invention discloses a method for extracting PN / PDRN from Lactobacillus bulgaricus and its application in soothing and anti-inflammatory effects. Belonging to the field of biochemistry, this method solves the problems of unstable PN / PDRN sources and susceptibility to contamination in existing technologies. In this invention, the preparation relies on alkaline conditions and lysozyme to initially dissolve the cell wall of Lactobacillus bulgaricus, and then sodium dodecyl sulfate is used to disrupt the bacterial cell membrane, causing it to lyse. The conditions are mild, and PVP is added to protect the PN structure. The final product prepared by this invention effectively improves the purity of the obtained PN / PDRN.

The present invention relates to the technical field of the development and utilization of probiotics, specifically to a Weissella confusa DY12 with probiotic activity and its application. The Weissella confusa DY12 was isolated from sheep feces. The present invention identified the probiotic effect of Weissella confusa DY12 through animal experiments. The results showed that Weissella confusa DY12 could tolerate acid and bile salts and resist the gastrointestinal internal environment; its hydrophobicity was 39.47% and its auto-aggregation was 48.12%, demonstrating that the Weissella confusa DY12 of the present invention has good intestinal adhesion ability and has the potential of probiotics. The present invention verified that Weissella confusa DY12 can effectively regulate the immune function of the animal body, improve the antioxidant capacity of the body, as well as the content of lysozyme and the activity of alkaline phosphatase, and increase the daily weight gain, ultimately improving the growth performance of animals.

The invention discloses a preserving fluid for stably preserving sample DNA at normal temperature and a preparation method thereof, and belongs to the technical field of biological sample preservation. The preserving fluid comprises a lysis system, a nucleic acid protection system and a buffering and stabilizing system, the cracking system comprises a composite surfactant and an enzymolysis auxiliary agent; the composite surface active agent is prepared from polyether polyol fatty acid ester and cocamidopropyl hydroxy sulfobetaine; the enzymolysis auxiliary agent comprises lysozyme Lyso-V and protease K; the nucleic acid protection system comprises a nitrogen heterocyclic polyamine-carboxylic acid derivative and dextran sulfate; the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N' ', N ''tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N''-triacetic acid, disodium ethylene diamine tetraacetate-nitrogen heterocyclic derivative, and diethylenetriamine pentaacetic acid-piperazine derivative, and the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N '', N'' tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N ''-triacetic acid. The buffering and stabilizing system comprises an amphoteric buffering agent and a polymer stabilizer; the amphoteric buffering agent comprises 2-(N-morpholino) ethanesulfonic acid and N-tri (hydroxymethyl) methylglycine.

The invention belongs to the technical field of drug sustained release, and particularly relates to a preparation method and application of a BMP-2 controllable sustained-release flocculent body based on lysozyme self-regulation. The preparation method comprises the following steps: preparing a chitosan solution; sequentially adding a protein stabilizer and glycerol into the chitosan solution obtained in the step A1 to prepare a chitosan composite solution; obtaining a non-specific binding solution; preparing BMP-2 / chitosan solid particles by adopting a freeze drying method; the preparation method comprises the following steps: preparing a PLGA / PEG composite solution to obtain a suspension, and carrying out post-treatment on the suspension to form the BMP-2 controllable slow-release flocculent. The carrier in the BMP-2 controllable slow-release flocculent body prepared by the preparation method can be specifically degraded by lysozyme in a human body, so that the release amount and the release speed of BMP-2 can be automatically adjusted according to a bone healing process, and long-time continuous release of the BMP-2 controllable slow-release flocculent body is realized. The problems that in the prior art, the slow release effect of a hydrophilic material on BMP-2 is poor, sudden release of factors is easily formed, and the slow release time and the slow release dosage are not controllable are solved.

The invention discloses a bacteriostatic composition and an application thereof in a hand sanitizer, the bacteriostatic composition comprises the following components by mass: 3-5% of lysozyme, 4-8% of an enzyme protection system, 8-12% of a plant bacteriostatic extract, 10-15% of a biological matrix carrier, 5-8% of a stable protection system, and the balance of water. According to the antibacterial composition, a three-dimensional network structure is formed through the carboxymethyl chitosan-sodium alginate compound, a protective layer with good biocompatibility can be formed on the skin surface, and the skin retention time of lysozyme and plant antibacterial active ingredients is remarkably prolonged. According to the invention, lysozyme and plant antibacterial components are adopted to form multiple antibacterial mechanisms: the lysozyme specifically hydrolyzes peptidoglycan in bacterial cell walls, and active components in the plant antibacterial extract destroy bacterial cell membranes and inhibit bacterial metabolism. The double-attack mechanism has a stronger antibacterial effect than a single component, and can prevent bacteria from generating drug resistance at the same time.

The invention relates to the technical field of microorganisms, in particular to plant lactobacillus BR32 with functions of preventing decayed teeth and improving inflammation and application of the plant lactobacillus BR32. The plant lactobacillus BR32 with the functions of preventing decayed teeth and improving inflammation, provided by the invention, has the preservation number of CGMCC No.32836, has an inhibiting effect on the growth of oral pathogens such as streptococcus mutans, candida albicans and porphyromonas gingivalis, is high in oxidation resistance, is beneficial to continuous oral cavity stability maintenance through a synergistic effect, also has relatively high hydrophobicity and self-agglutination capability, and can be used for preparing oral cavity health-care products. The compound is low in tooth environment corrosion, high in lysozyme tolerance and good in oral colonization capacity, does not have a hemolysis phenomenon in in-vitro hemolysis culture, is sensitive to common antibiotics and has relatively high food safety. The invention further provides a microbial agent, a probiotic product, a functional food, a fermented product, a bacteriostatic agent and an oral product containing the plant lactobacillus BR32 with the functions of preventing the decayed teeth and improving the inflammation, and the effects of preventing the decayed teeth, improving the inflammation, preventing dental plaque, relieving oral ulcer and the like are achieved.

The invention provides a preparation method of a hydrolyzed collagen product with surface amphipathicity as well as the product and application thereof, and the preparation method comprises the following steps: (1) taking hydrolyzed collagen enzymatic hydrolysate as a substrate, then preparing a fermentation culture medium with vegetable oil and sugar, and fermenting by adopting candida bumblebee; (2) after fermentation, adding lysozyme into the fermentation liquor for activating treatment, and then sterilizing, centrifuging and filtering; and (3) carrying out freeze-drying treatment on the filtered fermentation liquor to obtain the hydrolyzed collagen product with surface amphipathicity. The hydrolyzed collagen product prepared by the invention not only has low molecular weight and high permeability to skin, but also has water locking and moistening effects on the skin surface due to the amphiphilic sophorolipid on the surface, so that the moisture retention of the skin is better improved, and the product has excellent product characteristics and has wide market prospects.

The invention relates to an instant composition with a long-acting oral health-care function for dogs and cats as well as a preparation method and application of the instant composition, and belongs to the technical field of pet food and pet oral care. The composition comprises 20%-30% of whey protein powder, 10%-20% of hydrolyzed gelatin, 1%-3% of low-molecular-weight chitin saccharide gum, 1%-2% of modified cassava starch, 2%-5% of sodium alginate, 1%-3% of fructo-oligosaccharide, 0.03%-0.08% of lysozyme, 0.03%-0.08% of tea polyphenol, 0.05%-0.15% of zinc gluconate, 0.05%-0.1% of persimmon tannin, 3%-5% of tuna hydrolysate, 4%-6% of whole goat milk powder, 2%-4% of skim yoghurt powder and the balance water. The added active ingredients have long-acting slow-release and continuous effects in the formula, oral pathogenic bacteria can be effectively inhibited, dental plaque and dental calculus formation can be reduced, halitosis can be lastingly relieved, and the toothpaste has good market competitiveness.

The invention relates to the technical field of fermentation extraction, and particularly provides a PHA extraction and purification method, a PHA product and application, the PHA extraction and purification method comprises the following steps: (1) adding a surfactant I into a PHA fermentation broth, stirring while keeping the temperature, adding alkali until the feed liquid is alkaline, and carrying out mechanical wall breaking treatment to obtain a first feed liquid; (2) adding lysozyme into the first feed liquid, keeping the temperature, adding alkali until the feed liquid is alkaline, adding alkaline protease, keeping the temperature, and obtaining second feed liquid; and (3) adding a surfactant II into the second feed liquid, stirring and washing, wherein the surfactant II comprises SDS (sodium dodecyl sulfate), sodium lauryl sulfate and lauryl glucoside. The PHA product obtained by adopting the PHA extraction and purification method has excellent purity and whiteness, so that the PHA product has a wider application prospect, and the method is simple in process, convenient to operate and low in cost.

The invention discloses a lysozyme-xanthan gum conjugate nano-particle for improving bioavailability of pleurotus eryngii immune active peptide, which is a nano-particle loaded with pleurotus eryngii immune active peptide prepared by taking lysozyme, xanthan gum and pleurotus eryngii immune active peptide as raw materials through a Maillard reaction controllable damp-heat method. Wherein the amino acid sequence of the pleurotus eryngii immune active peptide is LLGVD. By combining the mild and controllable Maillard reaction with the ultrafiltration purification technology, high-proportion and high-activity loading of the pleurotus eryngii immunocompetent peptide is realized under the mild condition, and the structural integrity and immunomodulatory activity of the pleurotus eryngii immunocompetent peptide are effectively maintained; wide application prospects are realized in the aspect of developing functional foods or related products for improving low immunity. The physical stability, the digestion resistance and the potential oral bioavailability of the active peptide are remarkably improved, and the whole process is safe, green and relatively simple and has good amplification potential.

The invention discloses a molecularly imprinted adsorption material taking a metal organic framework as a carrier skeleton as well as a preparation method and application of the molecularly imprinted adsorption material, and belongs to the technical field of adsorption materials. The preparation method comprises the following steps: carrying out hydrothermal reaction on transition metal salt and a phthalic acid ligand to obtain metal organic framework nanoparticles; the preparation method comprises the following steps: adsorbing flavonoid template molecules by metal organic framework nanoparticles, mixing with lysozyme and a disulfide bond reducing agent, performing phase change polymerization, and eluting to remove the flavonoid template molecules after the phase change polymerization is completed, thereby obtaining the molecularly imprinted adsorbing material with abundant imprinting sites and good stability. The adsorbent is good in flavonoid compound adsorption selectivity, high in rate, large in capacity, good in chemical stability, resistant to acid washing, capable of being recycled for multiple times and capable of being widely applied to extraction and separation of flavonoid compounds.

The invention discloses a pregnant sow feed for increasing birth weight of piglets and improving placenta inflammation of sows, and relates to the technical field of biology. The pregnant sow feed comprises a lysozyme oligomer and a basic ration. The pregnant sow feed for increasing birth weight of piglets and improving placenta inflammation of sows provided by the invention is used for dynamically feeding sows in the pregnancy stage, meets the nutrition and energy requirements of the sows from the late pregnancy stage to delivery, and is fed for several times two days before delivery, so that the dystocia condition of the sows can be relieved, the stillbirth rate can be effectively reduced, and the survival rate of the sows is increased. The lysozyme content in colostrum and serum is increased, the anti-inflammatory factor expression of placenta is obviously promoted, the birth weight of piglets is obviously increased, the stillbirth rate is reduced, and the reproductive performance of sows is improved.

The invention discloses double-milk-source fermented milk of double-peak camel milk and brown milk and a preparation method of the double-milk-source fermented milk, and the preparation method comprises the following steps: mixing raw materials: mixing the double-peak camel milk and the brown milk according to a mass ratio to obtain double-milk-source mixed milk; pretreatment: sequentially carrying out homogenization treatment and heat treatment on the double-milk-source mixed milk; strain inoculation: inoculating the pretreated double-milk-source mixed milk with a fermentation strain, wherein the fermentation strain comprises a core strain and an auxiliary strain; the core strains are lactobacillus and streptococcus thermophilus which have tolerance to antibacterial components of the camel milk, and the core strains contain strains capable of producing exopolysaccharides. Through scientific matching of lactobacillus, streptococcus thermophilus and specific auxiliary strains which have tolerance to antibacterial components of the camel milk, the inhibition effect of natural antibacterial components such as lysozyme and lactoferrin in the camel milk on conventional strains is successfully overcome.

The invention relates to the technical field of high-activity immune globulin liquid extraction, in particular to a preparation method for extracting high-activity immune globulin liquid from bovine colostrum. The invention discloses a preparation method for extracting high-activity immune globulin liquid from bovine coloctrum, which comprises the following steps: performing primary separation, secondary separation, filtration, ultrafiltration concentration and sterilization on degreased bovine coloctrum to obtain the high-activity immune globulin liquid, the primary separation comprises the following steps: adjusting the pH value of the defatted bovine colostrum to 4.6-4.7, and sieving to remove casein, so as to obtain whey; and the secondary separation comprises the following steps: adjusting the pH value of the whey to 3.8-4.0, uniformly mixing, and sieving to remove denatured protein to obtain an immunoglobulin liquid. The high-activity immune globulin liquid containing IgG, IgA, IgM, lactoferrin, lactoperoxidase, IGF-beta, lysozyme, lactose and protein, which is prepared by adopting the method disclosed by the invention, can be used for effectively improving the immunity of a human body.

The invention relates to a multi-enzyme synergistic skin care composition suitable for sensitive skin and application thereof, the skin care composition comprises the following components: crithmum maritimum extract, pomegranate peel extract, lysozyme and coenzyme Q10, the crithmum maritimum extract is obtained by anaerobic fermentation of crithmum maritimum through leuconostoc mesenteroides and lactobacillus plantarum and is rich in SOD enzyme; the pomegranate peel extract is obtained by performing anaerobic fermentation on pomegranate fruits through lactobacillus helveticus and is rich in protease and lipase. The crithmum maritimum extract, the pomegranate peel extract, the lysozyme and the coenzyme Q10 are compounded for use, a multi-dimensional synergistic skin care system is constructed, and repairing, anti-inflammatory and anti-aging integrated efficient improvement is achieved.

The invention provides tooth enamel repairing toothpaste and a preparation method thereof, and the tooth enamel repairing toothpaste comprises the following components in percentage by mass: 15-30% of glycerol, 15-30% of glycerol, 5-10% of sodium dodecyl sulfate, 5-10% of sodium dodecyl sulfate, 5-10% of sodium dodecyl sulfate and the balance of deionized water. 10%-25% of hydrated silica; 3%-10% of polyethylene glycol-8; 3%-7% of calcium phosphosilicate; 1%-5% of a surfactant; 0.5%-3% of a binder; 0.05%-0.5% of an antibacterial agent; 0.1%-1.5% of a compound enzyme; the compound enzyme comprises lysozyme, papain and bromelain; the mass ratio of the lysozyme to the papain to the bromelain is 1: (0.4-0.6): (0.4-0.6); and the balance of water. The toothpaste can be used for remarkably improving the repairing effect of enamel and the oral health maintenance capability.

The invention provides a layer-by-layer self-assembled gallium-lysozyme composite antibacterial stainless steel coating and application thereof, and relates to the technical field of medical equipment. According to the composite antibacterial stainless steel coating, medical 316L stainless steel is used as a substrate material, a dopamine solution is used as a raw material, Tris salt is added to initiate a polymerization reaction of dopamine, and a polydopamine film is formed on the surface of the stainless steel; the preparation method comprises the following steps: carrying out Michael addition reaction on an easily oxidized catechol group and lysozyme containing amino and sulfydryl in an aerobic environment to form stable covalent linkage, thereby preparing the lysozyme-loaded polydopamine composite membrane. In the invention, the antibacterial composite coating aims to effectively kill biofilm embedding bacteria and prevent bacterial adhesion and biofilm formation on the surface of an implant under the condition of not hurting an organism, so that the antibacterial property of the implantable stent is improved, and the problem of clinical postoperative explosive infection of the implant is solved; and more thoughts and means and beneficial exploration are provided for treatment of chronic refractory inflammation caused by biological membranes.

The invention relates to the technical field of treatment of thallus mash, in particular to a treatment method and system of thallus protein mash and application of the treatment method and system. The treatment method comprises the following steps: carrying out vacuum concentration on mycoprotein mash to obtain concentrated protein mash; carrying out wall breaking treatment on the concentrated protein mash to obtain concentrated protein mash containing thallus fragments; carrying out neutralization reaction on the concentrated protein mash by using an alkaline solution to obtain a concentrated protein reaction solution; sequentially carrying out enzymolysis reaction and enzyme deactivation treatment on the concentrated protein reaction solution by using a compound enzyme system to obtain a proteolysis reaction solution; wherein the compound enzyme system comprises the following raw materials: lysozyme, alkaline protease, nuclease and flavourzyme; and carrying out purification treatment on the proteolysis reaction liquid to obtain the mycoprotein treatment liquid. According to the treatment method, operations such as vacuum treatment, wall breaking treatment, neutralization reaction treatment and enzymolysis reaction are combined, and the content of peptide substances in the mycoprotein mash is effectively increased to 25% or above.

The invention relates to the technical field of enzyme preparations, in particular to an agricultural biological compound enzyme preparation and a preparation method thereof.The agricultural biological compound enzyme preparation is prepared from, by weight, 30-50 parts of compound enzyme, 5-8 parts of trehalose, 3-5 parts of glycerin, 15-20 parts of copolymer carrier, 5-8 parts of humic acid, 5-10 parts of bran, 3-5 parts of dipotassium phosphate and 85-124 parts of water. The compound enzyme contains various enzymes such as cellulase, lysozyme and protease, can synchronously decompose various organic substances such as cellulose, microbial cell walls and protein in soil and release nutrients such as nitrogen, phosphorus and potassium, has more comprehensive functions compared with a single enzyme preparation, and can meet the requirements of complex soil and crops in agricultural production.

The invention discloses a trichoderma reesei engineering bacterium for secreting and expressing egg white lysozyme and a construction method of the trichoderma reesei engineering bacterium, and belongs to the field of molecular biology and biotechnology. The invention firstly provides an egg white lysozyme mutant, and the amino acid sequence of the egg white lysozyme mutant is shown as any one of SEQ ID NO.2-4. The construction method of the trichoderma reesei engineering bacterium for secreting and expressing the egg white lysozyme comprises the following steps: by taking trichoderma reesei as a starting strain, transforming an expression module into the trichoderma reesei to construct the trichoderma reesei engineering bacterium; the expression module comprises a promoter, a secretory peptide gene, an egg white lysozyme gene and a terminator which are connected in sequence. The coding gene of the egg white lysozyme is transformed into a trichoderma reesei genome, so that a trichoderma reesei engineering strain for secreting and expressing the egg white lysozyme is successfully constructed. Meanwhile, different egg white lysozyme variants can bring different effects, and when the trichoderma reesei RUT-C30 is used as an original strain, the effect is superior to that of QM6a.

The invention discloses a feed low-temperature sterilization method based on a composite biological enzyme synergistic effect. The feed low-temperature sterilization method comprises the following steps: a pretreatment stage; a dynamic reaction stage: under the conditions that the temperature is 40-50 DEG C and the humidity is 60%-70%, the enzyme catalytic reaction lasts for 30 minutes, and the microbial structure is decomposed; and inactivating the activity of the residual enzyme through short-time pulsed ultraviolet rays. The compound enzyme comprises lysozyme, thermophilic protease and lipoxygenase, and the nano chitosan carrier and the compound enzyme are mixed according to the mass ratio of 1: 1. According to the method, damage to heat-sensitive nutritional ingredients is avoided through low-temperature operation (40-50 DEG C), broad-spectrum sterilization is achieved through the synergistic effect of compound enzymes, multiple microorganisms such as bacteria, molds and spores are covered, meanwhile, it is guaranteed that no chemical residues exist through the ultraviolet inactivation technology, and animal health and environment safety are guaranteed. The method is low in equipment cost, easy and convenient to operate and free of public acceptance problems, a green, efficient and economical solution is provided for the feed processing industry, and the efficiency and safety of the feed sterilization technology are remarkably improved.

The invention relates to the technical field of non-woven fabric preparation, in particular to biochemical modified antibacterial non-woven fabric and a preparation method thereof.After nano-zinc oxide and nano-titanium dioxide are modified through a silane coupling agent KH-570, the dispersity of nano-particles can be improved, and the antibacterial property of the non-woven fabric is improved; under illumination or contact conditions, active oxygen is released to destroy bacterial cell membranes and inhibit metabolism of the bacterial cell membranes, so that the antibacterial modified polypropylene master batch has stable antibacterial property; loading is stabilized through a polydopamine coating, so that the antibacterial performance of the lysozyme is stabilized, the lysozyme can specifically hydrolyze a peptidoglycan layer of a bacterial cell wall to cause bacterial lysis, thiourea dioxide serving as a reducing agent can maintain the activity of the lysozyme, oxidative inactivation is avoided, meanwhile, the activity of bacterial metabolic enzymes is inhibited, and the antibacterial activity of the lysozyme is improved. Through the inorganic / biological double-effect synergistic effect, gram-positive bacteria, gram-negative bacteria and fungi can be covered, so that the antibacterial performance of the non-woven fabric is improved to a certain extent.