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181 results about "Lysozyme" patented technology

Lysozyme, also known as muramidase or N-acetylmuramide glycanhydrolase, is an antimicrobial enzyme produced by animals that forms part of the innate immune system. Lysozyme is a glycoside hydrolase that catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan, which is the major component of gram-positive bacterial cell wall. This hydrolysis in turn compromises the integrity of bacterial cell walls causing lysis of the bacteria.

Lysozyme, antibacterial peptide derived from lysozyme and application of lysozyme in preparation of antibacterial drugs

The invention discloses lysozyme, antibacterial peptide derived from the lysozyme and application of the lysozyme in preparation of antibacterial drugs. According to the invention, 1, 640 protein sequences recorded in a ruminant rumen caterpillars genome database are systematically predicted based on a lysozyme prediction model, and finally, the lysozyme with the amino acid sequence as shown in SEQ ID No.1 is relatively strong in antibacterial activity and stable in antibacterial activity, and is slightly influenced by environmental factors. According to the invention, an antibacterial peptide region of the lysozyme is analyzed and determined, three peptide fragments with clear antibacterial activity are identified, and the amino acid sequences of the peptide fragments are respectively shown as SEQ ID No.2, SEQ ID No.3 or SEQ ID No.4. The antibacterial peptide provided by the invention has relatively strong membrane destroying ability and broad-spectrum antibacterial activity, and compared with integral protein, the antibacterial peptide is simpler in structure and more flexible in synthesis and modification, and is easier to break through a bacterial drug-resistant mechanism.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI

Instant composition with long-acting oral health-care function for dogs and cats as well as preparation method and application of instant composition

The invention relates to an instant composition with a long-acting oral health-care function for dogs and cats as well as a preparation method and application of the instant composition, and belongs to the technical field of pet food and pet oral care. The composition comprises 20%-30% of whey protein powder, 10%-20% of hydrolyzed gelatin, 1%-3% of low-molecular-weight chitin saccharide gum, 1%-2% of modified cassava starch, 2%-5% of sodium alginate, 1%-3% of fructo-oligosaccharide, 0.03%-0.08% of lysozyme, 0.03%-0.08% of tea polyphenol, 0.05%-0.15% of zinc gluconate, 0.05%-0.1% of persimmon tannin, 3%-5% of tuna hydrolysate, 4%-6% of whole goat milk powder, 2%-4% of skim yoghurt powder and the balance water. The added active ingredients have long-acting slow-release and continuous effects in the formula, oral pathogenic bacteria can be effectively inhibited, dental plaque and dental calculus formation can be reduced, halitosis can be lastingly relieved, and the toothpaste has good market competitiveness.
Owner:JIANGSU CHOMPING PET PROD CO LTD

HPV sterilization underwear laundry detergent containing lysozyme-antibacterial protease and preparation process of HPV sterilization underwear laundry detergent

The invention belongs to the technical field of laundry detergents, and particularly relates to an HPV sterilization underwear laundry detergent containing lysozyme-antibacterial protease and a preparation process. The HPV sterilization underwear laundry detergent containing lysozyme-antibacterial protease comprises the following components in percentage by mass: 12%-18% of modified alkyl glycoside, 4%-6% of an auxiliary surfactant, 1.5%-3% of a lysozyme-antibacterial protease compound, 0.8%-1.5% of double-chain quaternary ammonium salt, 2%-3% of sodium citrate, 1%-2% of glycerol, 1%-1.5% of citric acid, 0.1%-0.2% of a preservative, 0.1%-0.3% of essence and the balance of deionized water. The laundry detergent disclosed by the invention is mild in effect, short in decontamination time and good in decontamination effect, especially has an excellent decontamination effect on protein and sebum, and is especially suitable for decontaminating underwear.
Owner:JINOU (GUANGDONG) BIOTECHNOLOGY CO LTD

Method for rapidly counting staphylococcus aureus based on fluorescence confinement discretization

The invention belongs to the field of biosensing and microbiological detection, and relates to a staphylococcus aureus rapid counting method based on fluorescence confinement discretization. According to the method, oat bran is used as a natural carbon source, lysozyme active fragments are used as targeting ligands, targeting carbon quantum dots are synthesized through a one-step hydrothermal method, the targeting carbon quantum dots are modified by fluorescent dye SRB to obtain luminescence enhanced carbon quantum dots, and the luminescence enhanced carbon quantum dots can be specifically combined with cell walls of staphylococcus aureus, so that the staphylococcus aureus has stable fluorescence signals. And then, by taking the marked thalli as a core layer and a high-molecular polymer as a shell layer, preparing a core-shell nanofiber membrane by adopting a coaxial electrostatic spinning technology to realize spatial confinement and monomer discretization of the thalli, so that a group fluorescence signal is converted into a single-thalli distinguishable signal, and rapid and sensitive quantitative detection of the staphylococcus aureus is realized. The method is simple, convenient, high in sensitivity, suitable for rapid visualization and quantitative detection of staphylococcus aureus in foods such as dairy products, meat products and beverages, and wide in application prospect.
Owner:JIANGSU UNIV

Xanthomonas bacteria lysogenic bacteriophage

In order to control plant diseases caused by Xanthomonas bacteria, a novel bacteriophage exhibiting bacteriolytic activity specific to the same bacteria is isolated, and a plant disease control composition containing the same as an active ingredient is developed and provided. Provided are a lysozyme containing a bacteriophage having a novel genomic DNA sequence and exhibiting a lysolytic activity specific to Xanthomonas bacteria, and a plant disease control composition containing the lysozyme as an active ingredient.
Owner:KANEKA CORP +2

Insect lysozyme capable of preventing and treating rice virus diseases and application thereof

PendingCN121991928AExpand application boundariesEnhanced inhibitory effectBiocideEnzymesDispersal vectorPlant growth
The invention discloses insect lysozyme capable of preventing and treating rice virus diseases and application of the insect lysozyme. According to the invention, three pieces of lysozyme (RdLYZI1, RdLYZC1 and RdLYZC2) with remarkable inhibitory activity on various rice viruses are innovatively excavated from the body of a natural transmission vector insect of the viruses, and the amino acid sequences of the lysozyme are shown as SEQ ID NO.13, SEQ ID NO.14 and SEQ ID NO.15. The invention further discloses a preparation method of the lysozyme. Experimental verification shows that the lysozyme RdLYZI1, the lysozyme RdLYZC1 and the lysozyme RdLYZC2 can effectively inhibit replication and accumulation of various rice viruses in plants and vector insects, have a remarkable prevention and treatment effect on related virus diseases and relieve plant growth obstacles caused by the viruses. The invention provides a novel biological prevention and control strategy which is environment-friendly and high in targeting property for green prevention and control of rice virus diseases, and has an important application prospect.
Owner:FUJIAN AGRI & FORESTRY UNIV

Bacteriostatic mouthwash as well as preparation method and application thereof

The invention discloses antibacterial mouthwash as well as a preparation method and application thereof, and belongs to the technical field of daily chemical products. According to the invention, egg white lysozyme, ferulic acid and grape seed procyanidine are taken as core components, and the mouthwash with an efficient bacteriostatic effect is developed. And the antibacterial rates of streptococcus mutans and porphyromonas gingivalis are 96.88% and 96.30% respectively. Researches show that bacterial biofilms can be destroyed by using the mouthwash, and the biofilm quantity is reduced by 53.7% after the bacterial biofilms are treated for 24 hours; extracellular polysaccharide is inhibited, the content of water-insoluble glucan is reduced by 62.3%, and synthesis of a key cariogenic matrix is hindered; the acid production capacity is regulated and controlled, the pH change of 24-hour culture is only 1.50, and xylitol competitively inhibits glycolysis and cooperates with ferulic acid to block bacterial metabolism. The mouthwash prepared by the invention avoids the problem of enzyme activity inhibition of the ionic surfactant of the traditional mouthwash, takes effect more quickly, and provides a basis for developing natural, safe and efficient functional mouthwash.
Owner:HEBEI UNIV OF ENG

Phosphorus-fixing lanthanum-based nano composite material as well as preparation method and application thereof

The invention provides a phosphorus-fixing lanthanum-based nano composite material as well as a preparation method and application thereof, and relates to the field of novel nano composite materials. According to the material, lysozyme protein fibers are taken as a structural template, a confinement space is constructed by utilizing the ultrahigh length-diameter ratio of the lysozyme protein fibers, and controllable synthesis of the sub-5nm high-dispersion lanthanum oxide nanoparticles is realized. Functional groups in a protein chain can be enriched and nucleation sites can be provided through electrostatic attraction and key coordination synergistic effect, so that the lanthanum-based protein fiber nano composite material is formed. When the material is used for treating phosphorus wastewater, efficient phosphorus removal and long-term phosphorus fixation are realized through an enrichment-conversion synergistic mechanism: phosphate ions are enriched through electrostatic adsorption, and thermodynamically stable lanthanum phosphate is generated in situ at lanthanum active sites. According to the method, gt is still kept in the presence of high-concentration equal competitive ions; the phosphorus removal rate is 90%, the generated lanthanum phosphate can be used as a slow-release phosphate fertilizer or an industrial phosphorus chemical raw material, and closed-loop conversion from pollution purification to resource utilization is realized.
Owner:YANSHAN UNIV

Raw wet noodle making method adopting static magnetic field to assist fermentation and raw wet noodle fresh-keeping and cooking integrated device

The invention provides a preparation method of raw wet noodles fermented with assistance of a static magnetic field and a fresh-keeping and cooking integrated device for the raw wet noodles. The preparation method of the raw wet noodles comprises the following steps: mixing lysozyme into dough; and putting the dough containing the lysozyme into a static magnetic field for fermentation. Based on the synergistic effect of lysozyme and static magnetic field auxiliary fermentation, the prepared raw wet noodles have good antibacterial and anti-browning performance, the quality of the fresh wet noodles can be improved, in some excellent implementation modes, the browning inhibition rate of the prepared raw noodles reaches 37.092%, and the area proportion of black spots is reduced to 0.03%.
Owner:HEFEI UNIV OF TECH

A pet oral care spray and method of making the same

PendingCN122351130AMouth careActive protein
This invention relates to the field of pet care product technology, and discloses a pet oral care spray and its preparation method. The preparation method employs a low-temperature gradient stepwise dissolution process: glucose oxidase is pre-dissolved at low temperature to obtain pre-solution A; bovine colostrum powder and lactoferrin are dissolved at low temperature to obtain protein pre-solution B; honey, folic acid, and vitamin D3 are dissolved at room temperature to obtain excipient pre-solution C; lysozyme is added to protein pre-solution B and stirred to dissolve; then pre-solution A and excipient pre-solution C are combined sequentially, the pH is adjusted, and purified water is added to make up the volume; the mixture is filtered through a microporous membrane for sterilization, and then filled and sealed under inert gas protection. This preparation method effectively protects the biological activity of each active protein and enzyme, achieving a long shelf life without the need for additional chemical preservatives. The resulting spray does not contain irritating ingredients such as alcohol and menthol, and is suitable for improving stomatitis, inhibiting plaque and tartar, and alleviating halitosis in pets of all ages and breeds.

Preparation method and application of near-infrared light-enhanced response Cu, Fe-Lyz / AgNPs nanoscale enzyme

The application discloses a preparation method of near-infrared light enhanced response Cu, Fe-Lyz / AgNPs nanoscale enzyme. The method uses Cu, Fe doped carbon dots as a reducing agent to prepare silver nanoparticles, and then the silver nanoparticles are modified by lysozyme to prepare Cu, Fe-Lyz / AgNPs nanoscale enzyme with positive charges on the surface. The Cu, Fe-Lyz / AgNPs nanoscale enzyme has dual activities of peroxidase-like and glutathione oxidase-like and photo-thermal performance. Meanwhile, the near-infrared light can significantly enhance the POD-like activity of the nanoscale enzyme. The Cu, Fe-Lyz / AgNPs nanoscale enzyme has good antibacterial effect on escherichia coli and staphylococcus aureus. Meanwhile, based on the enhancement of the penicillin G potassium on the Cu, Fe-Lyz / AgNPs oxidase-like activity, the application establishes a rapid colorimetric detection method for the penicillin G potassium, and when the method is used for detecting the penicillin G potassium in blood plasma and milk powder samples, the detection limit is as low as 0.05 mu g / mL, and the method has high reliability and accuracy.
Owner:KUNMING UNIV OF SCI & TECH

Oxidoreductase-containing composition for cosmetics as well as preparation method and application of oxidoreductase-containing composition

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.
Owner:广州柏为科技有限公司

Extraction and purification method of phytosphingosine synthesized by whole cell fermentation

The application provides an extraction and purification method for synthesizing phytosphingosine through whole-cell fermentation, which comprises the following steps: performing enzymolysis, ceramic membrane permeation and concentration on a fermentation liquor to obtain a bacteria slurry liquor; adding a strong base to the bacteria slurry liquor and performing primary hydrolysis under heating to obtain a hydrolysis liquor; adjusting the pH value of the hydrolysis liquor to 7-8, and then performing extraction, centrifugation, concentration, purification, purification, filtration and drying to obtain high-purity N-acetyl phytosphingosine; adding the high-purity N-acetyl phytosphingosine into an ethanol aqueous solution, adding a strong base into the ethanol aqueous solution, and performing secondary hydrolysis under heating to obtain a phytosphingosine reaction liquor; and performing crystallization, recrystallization, centrifugation and drying on the phytosphingosine reaction liquor to obtain the phytosphingosine. The extraction and purification method releases the cell contents by using lysozyme, removes water-soluble impurities through a ceramic membrane, and retains tetraacetyl phytosphingosine phytosphingosine, and is efficient and fast.
Owner:云合(天津)生物技术有限公司

Bacillus fermentation product for repair and moisturization, and preparation method and application thereof

The application belongs to the field of cosmetics, and particularly relates to a bacillus fermentation product for repairing and moisturizing, and a preparation method and application thereof. The preparation method of the bacillus fermentation product for repairing and moisturizing comprises the following steps: S1, culturing bacillus in a culture medium to obtain bacillus spores; S2, treating the bacillus spores obtained in step S1 with lysozyme and protease, and then treating the bacillus spores with an ultrahigh-pressure homogenizer to obtain a spore lysis product; and S3, centrifuging, concentrating and heating the spore lysis product obtained in step S2 to obtain the bacillus fermentation product for repairing and moisturizing. The spore lysis product is applied to the field of cosmetic raw material preparation, and the bacillus fermentation product obtained by the application has excellent repairing and moisturizing effects, can inhibit staphylococcus aureus, and has the effect of balancing skin microecology.
Owner:QUANHOU (GUANGZHOU) BIOTECHNOLOGY RES INST CO LTD

Method for detecting lysozyme content in food

The application discloses a lysozyme content detection method in food, and belongs to the technical field of biological medicine detection. The method comprises the following steps: constructing a lysozyme detection standard curve; reacting a food sample to be detected with a fluorescent probe in a buffer system, measuring the fluorescence intensity of the reaction system, and calculating the lysozyme content in the food sample to be detected based on the standard curve. The application adopts a fluorescence spectrum method to research and compare the performance of polypeptide CALNNK (FITC) and lysozyme under different buffer systems, pH, temperature and reaction time conditions, and then uses the method to detect the lysozyme content in food. The 20 nmol / L polypeptide CALNNK (FITC) , BR buffer solution with pH=10.0, 4 min of time and 35 DEG C of temperature are the optimal reaction conditions, the lysozyme content in food can be quickly and accurately detected, and the method has far-reaching significance for the healthy development of the food industry.
Owner:LINGNAN NORMAL UNIV

Large-particle dog and cat oral health-care functional food wrapped with instant freeze-dried particles and preparation method of large-particle dog and cat oral health-care functional food

The invention relates to a large-particle dog and cat oral health-care functional food wrapped with instant freeze-dried particles and a preparation method thereof, and belongs to the technical field of pet food. According to the scheme, an external spraying system comprises grain base material particles, 0.1 to 0.15 part of lecithin, 0.002 to 0.003 part of monostearin, 0.001 to 0.003 part of vitamin E and 0.8 to 1.2 parts of chicken oil, and an instant freeze-dried particle layer comprises 0.1 to 0.2 part of fucoidan, 0.02 to 0.05 part of lysozyme, 0.04 to 0.08 part of tea polyphenol, 0.02 to 0.04 part of zinc gluconate, 0.05 to 0.1 part of Arabic gum, 0.03 to 0.1 part of glutamine, 0.01 to 0.02 part of vitamin B2, 0.01 to 0.04 part of vitamin B12 and 0.02 to 0.07 part of glycine. The toothpaste can effectively inhibit the formation of dental plaque and dental calculus and remove halitosis.
Owner:SHANDONG LUSI PET FOOD

Biomass antibacterial hemostatic bandage and functionalization method thereof

The present application relates to the technical field of medical bandage, and discloses a biomass antibacterial hemostatic bandage and a functionalization method thereof.The bandage comprises a bandage base material and a composite antibacterial functional layer loaded thereon, the functional layer is composed of a composite antibacterial unit and lysozyme, and the composite antibacterial unit comprises a cationic antibacterial core, a polyphenol sustained-release shell and an enzymatic cellulose scaffold.The present application forms a biomass antibacterial hemostatic bandage with broad-spectrum, long-acting, low drug resistance and hemostatic function by constructing a cationic antibacterial core-polyphenol sustained-release shell-enzymatic cellulose scaffold composite antibacterial unit and synergistically loading lysozyme.The bandage shows significant technical advantages in dealing with complex wound infection, especially gram-negative bacterial infection and drug-resistant bacterial challenge, and provides an efficient and safe solution for clinical wound management, and has important clinical application value and broad market prospect.
Owner:YUNGUANG TECH (SHENZHEN) CO LTD

Toner, lysozyme buccal tablet and preparation method of lysozyme buccal tablet

PendingUS20260191775A1DentistryBiomedical engineering
The present application relates to the technical field of medicine processing, in particular to a toner, a lysozyme buccal tablet and a preparation method of the lysozyme buccal tablet. The toner is obtained by formulating a pigment suspension through adding purified water into the pigment, then atomizing and spraying the pigment suspension onto dextrin, mixing thoroughly, drying and pulverizing. The application further discloses a lysozyme buccal tablet and a preparation method thereof.
Owner:HANGZHOU GUOGUANG PHARMA

A composite lysozyme oral bacteriostatic repairing film and a preparation method thereof

PendingCN122351443AInorganic saltsArginine
This application relates to the fields of oral care and biopharmaceutical technology, and discloses an oral antibacterial and repairing film containing a compound lysozyme and its preparation method. The film components include sodium hyaluronate, poloxamer 407, lysozyme, L-arginine, D-trehalose dihydrate, L-methionine, glycerol, deoxygenated purified water, and a pH adjuster. The formulation utilizes L-arginine to competitively bind to the carboxyl group of sodium hyaluronate, blocking the electrostatic complexation and aggregation of polyanions and alkaline lysozyme. Combined with an inorganic salt avoidance design and a poloxamer network, it achieves a rheological response where the storage modulus increases in the opposite direction upon encountering salivary ions, enhancing mucosal retention. The oxygen consumption of L-methionine and the hydration effect of trehalose further preserve enzyme activity. The preparation method employs two-phase isolated formulation and continuous static mixing in tubular tubing to avoid high molecular weight aggregation. This invention solves the problems of easy inactivation and phase separation of lysozyme, improving the physical stability and antibacterial activity of the formulation.
Owner:NANJING STOMATOLOGICAL HOSPITAL

Method for removing earthy smell of tilapia fillets

The present application belongs to the technical field of food processing, and relates to a method for removing earthy smell of tilapia fillets. The method comprises the following steps: covering the surface of the tilapia fillets with pleurotus eryngii powder, and placing the tilapia fillets in a deodorizing and fresh-keeping liquid for 30-60 min; the deodorizing and fresh-keeping liquid is composed of ginger powder 15-20 parts, cannabis seed protein 5-10 parts, lysozyme 0.5-1 part, tea polyphenol 4-8 parts and water 60-100 parts in terms of mass fraction. The present application solves the technical problem of long-term maintenance of fresh fish flavor when removing the earthy smell of tilapia fillets.
Owner:GUANGDONG OCEAN UNIVERSITY

A method for modifying an anion exchange membrane with lysozyme and THPS

The application discloses a method for modifying an anion exchange membrane by using lysozyme and THPS, which comprises the following steps: step 1, pretreating a commercial anion exchange membrane; step 2, adding a reaction solution 1 containing levodopamine and lysozyme into a feed liquid chamber of a modification device, and making one side of the pretreated original membrane contact with the reaction solution 1; after stirring reaction is completed at room temperature, the reaction solution is poured out, and the obtained membrane is washed with deionized water to obtain an E-Lys membrane; step 3, adding a reaction solution 2 containing THPS into the feed liquid chamber, and making the modified side of the E-Lys membrane contact with the reaction solution 2 to generate a Mannich-type reaction; after continuous reaction is completed at room temperature, the reaction solution is poured out, and the modified membrane is washed with deionized water to obtain an E-Lys@Thps membrane. The application obtains a green and environment-friendly anion exchange membrane with good anti-biological pollution performance, bacteriostatic performance and separation performance.
Owner:ZHEJIANG UNIV OF TECH

Phage, salmonella bacteria lysozyme, composition and method for controlling salmonella bacteria

One of the purposes of the present disclosure is to: (i) provide a novel bacteriophage having a lysolytic activity against a Salmonella bacterium such as S.Enteridis, or a lysolytic agent comprising the same; (ii) providing a bacteriophage having a broad range of hosts for Salmonella bacteria or a lysozyme comprising the same; (iii) providing a host-specific bacteriophage or an effective bacterial lysozyme of the Salmonella genus comprising the same; or (iv) providing a bacteriophage capable of effectively controlling S.Typhimurium, in particular, S.Typhimurium having a multidrug resistance, or a bacteriolytic agent containing the bacteriophage, which is capable of effectively controlling S.Typhimurium, in particular, S.Typhimurium having a multidrug resistance. The present disclosure provides a bacteriophage having a specific genomic DNA sequence, a Salmonella bacterial lysozyme comprising the same, and a composition comprising the same.
Owner:KANEKA CORP +2

Chimeric endolysin lys009-lys009cbd and engineering and use thereof

The application discloses a chimeric endolysin Lys009-Lys009CBD and engineering and application thereof, and belongs to the field of genetic engineering and protein engineering. The application constructs a chimeric endolysin Lys009-Lys009CBD with high activity, and the peptidoglycan hydrolysis activity of the chimeric endolysin Lys009-Lys009CBD on Pseudomonas aeruginosa is 5.30 times that of natural endolysin Lys009 and 27.98 times that of hen egg white lysozyme HEWL. A transmembrane peptide P81417 is further fused to perform transmembrane modification, and an engineering endolysin P81417-Lys009-Lys009CBD is obtained. The engineering endolysin can efficiently lyse Pseudomonas aeruginosa, Acinetobacter baumannii and the like, and has excellent thermal stability. The application enriches the resource library of high-activity endolysins, provides important experimental basis and technical support for developing an anti-drug-resistant bacteria treatment strategy, and also provides a new selection for clinical treatment of multiple drug-resistant bacteria infection.
Owner:SOUTH CHINA UNIV OF TECH

Virus lysozyme from deep sea cold spring as well as preparation method and application of virus lysozyme

The invention belongs to the technical field of biology, and discloses virus lysozyme from a deep sea cold spring as well as a preparation method and application of the virus lysozyme. The virus lysozyme provided by the invention comprises amino acid fragments of which the sequences are as shown in one or more of SEQ ID NO: 1-7. The virus lysozyme is derived from a deep sea cold spring virus, the sequence and the structure of the virus lysozyme can keep structural stability and activity in extreme environments such as low temperature and high pressure, and the virus lysozyme has relatively good bacterial cell wall lysis capability and bacteriostatic action; the method has important significance on research and development of bacteriostatic agents under special ecological or technological conditions such as low-temperature food preservation, biological processing and ocean engineering.
Owner:THIRD INSTITUTE OF OCEANOGRAPHY STATE OCEANI C ADMINISTRATION

Method for sterilizing vegetable protein beverage

PendingCN121730365ABiotechnologyHydroxytyrosol
The invention discloses a method for sterilizing a vegetable protein beverage, which comprises the following steps of: adding a composite antibacterial microsphere preparation into a vegetable protein beverage to be treated, uniformly mixing, and performing high-density CO2 treatment and magnetic induction electric field sterilization to obtain a vegetable protein beverage finished product, the composite antibacterial microsphere preparation is prepared by taking fucoidan and a gallic acid acylated quercetin-phospholipid conjugate as a substrate and loading lysozyme and hydroxytyrosol. High-density CO2 and a magnetic induction electric field are adopted for synergistic sterilization, so that the structure of a microbial film can be changed, metabolism is disturbed, a good matrix is provided for electric field sterilization, and the sterilization efficiency is further improved through electric breakdown, magnetic field refining of water molecular groups and heat effect; the composite microspheres are sensitive to an electric field, so that lysozyme and hydroxytyrosol can be triggered to be accurately and quickly released under the action of a magnetic induction electric field, stubborn spores can be efficiently killed, and the killing effect is improved by destroying cell wall structures and disulfide bonds of the spores; meanwhile, protein aggregation and flocculation in the beverage can be effectively prevented, and the stability of a beverage system is guaranteed.
Owner:HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY +1

Flexible amyloid fiber gel based on albumin and lysozyme as well as preparation method and application of flexible amyloid fiber gel

The invention discloses flexible amyloid fiber gel based on albumin and lysozyme as well as a preparation method and application of the flexible amyloid fiber gel, and belongs to the field of delivery of natural high polymer materials and bioactive substances. The method comprises the following steps: respectively preparing ovalbumin fibers (OVAf) and lysozyme fibers (LYZf), mixing according to a volume ratio of 2: 1, adding resveratrol with a final concentration of 100 [mu] mol / L, and incubating at 37 DEG C for 3 h to form flexible gel; according to the gel, ordered crosslinking of fibers is induced through resveratrol, a uniform three-dimensional network is formed, and the gel has good elasticity and flexibility; when being used as a carrier to embed active ingredients such as anthocyanin, the nano-carrier can significantly improve the storage stability and digestion environment tolerance, and has excellent biocompatibility. The invention provides a new scheme for efficient delivery of bioactive components, and can be applied to the fields of functional foods, biological medicines and the like.
Owner:SHAANXI UNIV OF CHINESE MEDICINE

A composition for reducing gastroesophageal reflux in an individual, and methods of making and using the same

PendingCN122350335ATissue repairFood technology
The present application relates to the field of food technology, and specifically discloses a composition for reducing gastroesophageal reflux in individuals, a preparation method and application thereof. The composition for reducing gastroesophageal reflux in individuals comprises beta-defensin 1, lysozyme and lactoperoxidase. The present application constructs a multi-target synergistic intervention mechanism combining physical protection, secretion regulation and tissue repair through the compounding of four core components, which can comprehensively block the occurrence and development of gastroesophageal reflux. Lactadherin constructs a physical barrier to reduce the risk of reflux dynamics from the source; MLCT precisely regulates gastric acid secretion to reduce chemical stimulation; HMO complex IgG strengthens the mucosal immune barrier and promotes damage repair. The above composition is applied to products for reducing gastroesophageal reflux in individuals, and multi-target synergistic effect is achieved, realizing a full-chain closed-loop intervention from prevention, relief to repair, and significantly improving the clinical improvement effect.
Owner:JUNLEBAO DAIRY GRP CO LTD

Bionic reticulated nanofiber composite coating, preparation method thereof and application in repairing infectious bone defects

The present application relates to a kind of biomimetic reticular nanofiber composite coating and its preparation method and infectious bone defect repair application, belong to biomedical material field.The present application is aimed at solving the technical problem of the poor bone integration of implant in infectious bone defect.The present application provides a kind of biomimetic reticular nanofiber composite coating, which is formed by layer-by-layer self-assembly on the surface of implant by lysozyme amyloid fiber and polyphenols (such as tannin etc.) Form reticular structure, then prepared with bioactive metal ions coordination;The number of coating layers is 4-8 layers.Preparation method includes lysozyme fiberization, alternate deposition and ion loading steps.The composite coating of the present application has broad-spectrum antibacterial, antioxidant stress and promotes osteogenesis function;In animal model, bacterial load is reduced by 80%, bone volume fraction and trabecular bone parameters are significantly improved, and can realize anti-bacterial biofilm infection-antioxidant stress-angiogenesis-promote bone regeneration synergy.
Owner:WUHAN TEXTILE UNIV

A lysozyme amyloid fiber-based gel electrolyte and applications thereof

The present application relates to the technical field of gel electrolyte for supercapacitor, in particular to a gel electrolyte based on lysozyme amyloid fibers and application thereof. The ion conductivity of the conventional plant protein-based gel electrolyte is poor, and the electrical performance needs to be further improved. In view of the above problems, the present application provides a gel electrolyte based on lysozyme amyloid fibers. The lysozyme in the formula self-assembles into amyloid fibers under acidic conditions, and the surface of the amyloid fibers is rich in polar groups such as hydroxyl, carboxyl and amino groups, which can effectively promote the dissociation of lithium salt. At the same time, the three-dimensional network structure formed by the self-assembly of the fibers. Glycerol as a small molecule plasticizer inserts between the polymer chains. Lithium perchlorate and other lithium salts can provide high concentration of free lithium ions due to the large anion volume and low dissociation energy. The above components synergize and jointly act, effectively improving the comprehensive electrical performance of the gel electrolyte.
Owner:CHANGZHOU UNIV