12 results about "Dithiothreitol" patented technology

The application discloses a marine-derived cholesterol oxidase and a preparation method and application thereof, and relates to the technical field of biology. The amino acid sequence of the marine-derived cholesterol oxidase (SkChOx) is shown in SEQ ID NO. 1 or 3, the specific enzyme activity of the marine-derived cholesterol oxidase can reach 31.9 U / mg, the marine-derived cholesterol oxidase has excellent anti-reverse performance, maintains high stability in the pH range of 5.0-10.0, has good temperature tolerance, organic solvent tolerance and metal ion tolerance, and exhibits unique salt activation effect and salt tolerance. The enzyme is compounded with dithiothreitol (DTT) and nonylphenol polyoxyethylene ether (NP-40) to form a liquid enzyme preparation, and after incubation at 37 DEG C for 7 days under accelerated conditions, the catalytic activity of the liquid enzyme preparation still remains about 87%, the liquid enzyme preparation has excellent long-acting storage stability, and has wide application prospects in the fields of clinical diagnosis, food physical and chemical inspection, biosensor development and industrial preparation of steroid drugs.

The present invention relates to the technical field of polymer synthesis and relates to a preparation method for an oil-proofing agent based on polymerization of acrylic acid and organosilicon. In the present invention, the method comprises: adding methyl acrylate, hydroxyethyl acrylate, dithiothreitol, m-trifluoromethylcinnamaldehyde, and triethylamine to a reaction kettle, and stirring and heating the mixture for a reaction; adding an initiator, and continuing the reaction, so that the acrylic monomers undergo a polymerization reaction to form a polymer having good stability and mobility; further adding α,ω-dihydroxypolydimethylsiloxane and continuing the reaction, so that the linear organosilicon and the polymer undergo a polymerization reaction; adding an emulsifier, and stirring and mixing the mixture for a reaction to obtain an oil-proofing agent; and adding the oil-proofing agent to a pulp for food boxes and uniformly stirring the mixture, so that the polymer is uniformly distributed on the surface of the pulp for food boxes to form a dense oil-proofing film, thereby effectively preventing the adhesion of oil stains. The oil-proofing agent prepared in the present invention has good oil-proofing performance, and can effectively prevent the adhesion of oil stains, thereby maintaining the cleanliness of food packaging and catering utensils.

This invention relates to a triboelectric nanogenerator and its preparation method, belonging to the technical field of triboelectric nanogenerators. The preparation method includes the following steps: S1, dispersing styrene-ethylene-butene-styrene block copolymer, polyvinylidene fluoride, and MXene nanomaterials in a mixed solvent to obtain a negative friction layer spinning solution; S2, using dynamic pattern-induced multi-material nanofiber electrospinning technology, forming a negative friction layer from the negative friction layer spinning solution; S3, dispersing thermoplastic starch, sodium carboxymethyl cellulose, plasticizer, bis(2-hydroxyethyl) disulfide, and dithiothreitol in water to obtain a positive friction layer film-forming solution; S4, forming a positive friction layer from the positive friction layer film-forming solution; S5, bonding the micro-nano structures of the positive and negative friction layers face-to-face to obtain the triboelectric nanogenerator. This generator combines high output, long lifespan, and flexibility, and can be adapted to diverse scenarios such as wearable electronics, industrial high-temperature sensing, and environmental mechanical energy harvesting.

This invention discloses a rapid detection kit for HAP / VAP pathogens and drug resistance genes, and its applications, belonging to the field of biomedical detection technology. Addressing the problems of long detection processes, complex operations, and poor sample adaptability in existing tNGS technology, this invention provides an optimized detection kit. The kit includes: a sample pretreatment and nucleic acid extraction reagent system, whose sample preservation solution contains 5% dithiothreitol and 2.5% potassium azide for simultaneous sample liquefaction and salt ion chelation during transportation; and an amplification and library construction reagent system, which contains a primer mixture consisting of 175 primer pairs, E. coli synthase III, and separately added magnesium ions. By integrating the above reagent systems and optimizing the entire process, this invention reduces the total time from sample pretreatment to report generation to less than 2.5 hours. This invention has the advantages of extremely fast detection speed, wide sample applicability, high operational stability, and ease of automation.

Presented herein are methods for making, isolating, and purifying N-acetylcysteine amide, (2R,2R′)-3,3′-disulfanediyl bis(2-acetamidopropanamide, diNACA), intermediates and derivatives thereof comprising: alternatively contacting cystine with methanol and a chlorinating reagent to form an organic solution containing L-cystine dimethylester dihydrochloride; combining dried or undried L-cystine dimethylester dihydrochloride with a triethylamine, an acetic anhydride, and an acetonitrile to form a di-N-acetylcystine dimethylester; mixing dried di-N-acetylcystine dimethylester with ammonium hydroxide to form a di-N-acetylcystine amide (diNACA); and separating dried di-N-acetylcystine dimethylester into N-acetylcysteine amide with dithiothreitol, triethylamine, and an alcohol.

This invention belongs to the field of surgical wound repair technology, and provides a multifunctional composite hydrogel, its preparation method, and its applications. The invention involves mixing fibrinogen, mussel byssal protein, calcium chloride, methacrylated dextran (Dextran-MA), thrombin, dithiothreitol, and a dispersing solvent. The resulting mixture is then gelled to obtain a multifunctional composite hydrogel. Thrombin specifically promotes the cross-linking of fibrinogen to form a biomolecular network; dithiothreitol efficiently mediates the formation of an organic macromolecular network through a Michael addition reaction of Dextran-MA; the introduction of Mfp5, utilizing its abundant catechol groups to form multiple coordination and covalent cross-links with organic / biomolecular macromolecules, significantly enhances the interfacial adhesion strength of the multifunctional composite hydrogel; simultaneously maintaining the biocompatibility, biodegradability, hemostatic properties, anti-inflammatory effects, and healing-promoting capabilities of the multifunctional composite hydrogel.

This invention discloses a neutral dissociation agent and its preparation method for detecting the content of 25-hydroxyvitamin D in human blood samples, belonging to the field of biomedical detection technology. The dissociation agent is composed of disodium EDTA, sodium dodecyl sulfonate, dithiothreitol, hexadecyltrimethylammonium bromide, and hydroxypropyl-β-cyclodextrin dissolved in 50 mM tris(hydroxymethyl)aminomethane buffer, with the mass-volume ratios of each component being 0.5%-1%, 0.5%-1%, 0.2%-0.5%, 0.2%-0.5%, and 0.2%-0.5%, respectively. During preparation, each component is dissolved according to the steps, and the pH is adjusted to 7.4. This dissociation agent exhibits mild dissociation conditions, does not damage protein activity, has high dissociation efficiency, and is non-corrosive and non-toxic. The detection results show high specificity and sensitivity, with a coefficient of variation ≤6%. It can be directly integrated into automated detection processes and is suitable for large-scale clinical and research applications.

The application belongs to the technical field of genetic engineering, and relates to a Cas protein storage solution and application. The Cas protein storage solution comprises sucrose, a surfactant, dithiothreitol, glycerol and a bacteriostatic agent, wherein the mass-volume fraction of the sucrose is 1-10 %, the mass-volume fraction of the surfactant is 0.2-0.5 %, the mass-volume fraction of the dithiothreitol is 0.01-0.1 %, the mass-volume fraction of the glycerol is 30-60 %, and the mass-volume fraction of the bacteriostatic agent is 0.1-0.5 %. The storage solution provided by the application can obviously prolong the storage stability of Cas protein, and the preparation method is simple and low in cost.

This invention discloses a method for preparing a nano-copper-based conductive composite material, comprising the following preparation steps: Step 1, preparation of a poly(catechol-polyamine)-copper precursor suspension: S11, preparing a mixed monomer solution, wherein the mixed monomer solution contains a catechol-polyamine system, 3,4-dihydroxyphenylalanine, gallic acid, and Tween-80; S12, adjusting the pH of the mixed monomer solution to 8.5-9.0 using Tris-HCl buffer, and then sequentially adding copper sulfate, a composite oxidation system, dithiothreitol, and phytic acid. In this invention, through the synergistic polymerization of the catechol-polyamine system and 3,4-dihydroxyphenylalanine gallic acid, a poly(catechol-polyamine) coating layer with steric hindrance effect is formed on the surface of copper ions. Simultaneously, Tween-80 optimizes the hydrophilicity / hydrophobicity of the particle surface, and dithiothreitol regulates the charge distribution on the particle surface. These multiple effects fundamentally inhibit particle aggregation.

The application belongs to the technical field of biological medicine, and specifically discloses an ischemia modified albumin detection kit and method. The kit comprises reagent 1 and reagent 2; the reagent 1 comprises a water-soluble salt containing divalent cobalt ions and a surfactant, and has a pH of 7.1-8.5; the reagent 2 comprises dithiothreitol and a reducing protective agent, and has a pH of 4.0-7.5; the reducing protective agent is selected from at least one of tartaric acid, 2-hydroxy succinic acid, tris(2-carboxyethyl) phosphine, thiourea, mannitol and sodium sulfite. The kit is used to detect ischemia modified albumin by an albumin-cobalt combination test method, has the advantages of strong stability, high precision, good clinical correlation with mainstream manufacturers, wide linear range and the like, and can well meet the needs of clinical examination.

This invention discloses a buffer solution for locked nucleic acid probe-based real-time quantitative PCR (RT-PCR). The buffer solution is composed of dithiothreitol, betaine, tetramethylammonium chloride, ammonium sulfate, tris(hydroxymethyl)aminomethane, magnesium chloride, a Tween-20 / glycerol mixture, and bovine serum albumin. The pH of the buffer solution is 7.0–9.0. The volume ratio of Tween-20 to glycerol in the Tween-20 / glycerol mixture is 1:80–120. By adding protective agents and promoters, adjusting the pH value, and adjusting the concentration of corresponding components, this buffer solution effectively protects the enzymes necessary for real-time quantitative PCR detection and improves the stability of modified probes in RTS. It is superior to other solutions for locked nucleic acid modified probes in RTS, improving detection sensitivity, fluorescence intensity, and result reliability. Therefore, this buffer solution has broad application prospects in detections requiring more stringent and precise data.

The application discloses a kind of digestive juice for separating gastric corpus stem cell and its use method, belong to stem cell separation technical field;The digestive juice includes the following concentration components:18.5~28 nM Na2HPO4, 40~48 nM KH2PO4, 460.5~530 nM NaCl, 1~15.2 nM KCl, 200~350.55 nM D-furan fructosyl-D-glucopyranoside, 400~605 nM D-glucitol, 2~4 nM ethylenediaminetetraacetic acid disodium and 2~3 nM 1,4-dithiothreitol.The application also discloses the use method of the above digestive juice.The digestive juice for separating gastric corpus stem cell and its use method of the application can solve the problems of low cell yield, low activity, insufficient purity and poor repeatability of existing digestive juice and supporting use method, and have wide application prospect.