34 results about "Dithiothreitol" patented technology

The invention belongs to the technical field of biomedical materials and regenerative medicine, and relates to collagen-based injectable fiber hydrogel as well as a preparation method and application thereof. According to the injectable fiber hydrogel, methacrylated collagen and dithiothreitol are subjected to a thiol-ene click reaction rapidly under the action of a photoinitiator through illumination, a primary cross-linked network is formed, and the injection and in-situ gel forming requirements are met; then under the conditions that the pH is 6.5-8.0 and the temperature is 30-39 DEG C, collagen molecules are self-assembled into a nanofiber structure, and a bionic network similar to a natural extracellular matrix is obtained. The hydrogel disclosed by the invention has the advantages of rapid gelation, structural biomimetic property and biological activity; residual sulfydryl endows the hydrogel with active oxygen scavenging capacity, so that oxidative stress can be relieved, and immune response can be regulated; the fibrotic structure promotes cell adhesion, migration and tissue regeneration.

The application discloses a marine-derived cholesterol oxidase and a preparation method and application thereof, and relates to the technical field of biology. The amino acid sequence of the marine-derived cholesterol oxidase (SkChOx) is shown in SEQ ID NO. 1 or 3, the specific enzyme activity of the marine-derived cholesterol oxidase can reach 31.9 U / mg, the marine-derived cholesterol oxidase has excellent anti-reverse performance, maintains high stability in the pH range of 5.0-10.0, has good temperature tolerance, organic solvent tolerance and metal ion tolerance, and exhibits unique salt activation effect and salt tolerance. The enzyme is compounded with dithiothreitol (DTT) and nonylphenol polyoxyethylene ether (NP-40) to form a liquid enzyme preparation, and after incubation at 37 DEG C for 7 days under accelerated conditions, the catalytic activity of the liquid enzyme preparation still remains about 87%, the liquid enzyme preparation has excellent long-acting storage stability, and has wide application prospects in the fields of clinical diagnosis, food physical and chemical inspection, biosensor development and industrial preparation of steroid drugs.

The invention provides a method for improving the purity of collagen in the fermentation process of engineering bacteria and application of the method, and relates to the technical field of fermentation. Dithiothreitol (DTT) is used as an exogenous additive for the first time to be applied to the process of producing collagen through strain fermentation, and it is found that DTT can relieve intracellular stress of yeast cells and effectively relieve the collagen degradation condition in the fermentation process, and a fermentation product with higher purity is obtained. The invention provides a novel method for producing collagen through fermentation of engineering bacteria, and the method is simple and easy to operate, can effectively improve the purity of a fermentation product, and has a wide application prospect.

The present invention relates to the technical field of polymer synthesis and relates to a preparation method for an oil-proofing agent based on polymerization of acrylic acid and organosilicon. In the present invention, the method comprises: adding methyl acrylate, hydroxyethyl acrylate, dithiothreitol, m-trifluoromethylcinnamaldehyde, and triethylamine to a reaction kettle, and stirring and heating the mixture for a reaction; adding an initiator, and continuing the reaction, so that the acrylic monomers undergo a polymerization reaction to form a polymer having good stability and mobility; further adding α,ω-dihydroxypolydimethylsiloxane and continuing the reaction, so that the linear organosilicon and the polymer undergo a polymerization reaction; adding an emulsifier, and stirring and mixing the mixture for a reaction to obtain an oil-proofing agent; and adding the oil-proofing agent to a pulp for food boxes and uniformly stirring the mixture, so that the polymer is uniformly distributed on the surface of the pulp for food boxes to form a dense oil-proofing film, thereby effectively preventing the adhesion of oil stains. The oil-proofing agent prepared in the present invention has good oil-proofing performance, and can effectively prevent the adhesion of oil stains, thereby maintaining the cleanliness of food packaging and catering utensils.

This invention relates to a triboelectric nanogenerator and its preparation method, belonging to the technical field of triboelectric nanogenerators. The preparation method includes the following steps: S1, dispersing styrene-ethylene-butene-styrene block copolymer, polyvinylidene fluoride, and MXene nanomaterials in a mixed solvent to obtain a negative friction layer spinning solution; S2, using dynamic pattern-induced multi-material nanofiber electrospinning technology, forming a negative friction layer from the negative friction layer spinning solution; S3, dispersing thermoplastic starch, sodium carboxymethyl cellulose, plasticizer, bis(2-hydroxyethyl) disulfide, and dithiothreitol in water to obtain a positive friction layer film-forming solution; S4, forming a positive friction layer from the positive friction layer film-forming solution; S5, bonding the micro-nano structures of the positive and negative friction layers face-to-face to obtain the triboelectric nanogenerator. This generator combines high output, long lifespan, and flexibility, and can be adapted to diverse scenarios such as wearable electronics, industrial high-temperature sensing, and environmental mechanical energy harvesting.

The invention relates to the technical field of collagen material degradation, and particularly discloses an in-vitro degradation method of a collagen biological material, which comprises the following steps: pretreating the collagen biological material to obtain a to-be-degraded sample; adding the enzymatic hydrolysate into a to-be-degraded sample, sealing, and degrading at 37 + / -2 DEG C to obtain a degradation solution; the enzymatic hydrolysate is prepared from the following components: 10 to 20 mg / L of collagenase, 3 to 6 mg / L of sorbitol, 1 to 2 mg / L of calcium chloride, 0.3 to 0.8 mg / L of dithiothreitol and the balance of a Tris-HCl buffer solution with the pH value of 7 to 8. The in-vitro degradation method of the collagen biological material provided by the invention has the advantages of good degradation stability, small batch-to-batch difference and the like, and can be used for investigating the degradation behavior of the collagen biological medical device against collagenase in vivo, so that research and development and quality control of the collagen biological medical device are realized.

This invention discloses a rapid detection kit for HAP / VAP pathogens and drug resistance genes, and its applications, belonging to the field of biomedical detection technology. Addressing the problems of long detection processes, complex operations, and poor sample adaptability in existing tNGS technology, this invention provides an optimized detection kit. The kit includes: a sample pretreatment and nucleic acid extraction reagent system, whose sample preservation solution contains 5% dithiothreitol and 2.5% potassium azide for simultaneous sample liquefaction and salt ion chelation during transportation; and an amplification and library construction reagent system, which contains a primer mixture consisting of 175 primer pairs, E. coli synthase III, and separately added magnesium ions. By integrating the above reagent systems and optimizing the entire process, this invention reduces the total time from sample pretreatment to report generation to less than 2.5 hours. This invention has the advantages of extremely fast detection speed, wide sample applicability, high operational stability, and ease of automation.

The application discloses a method and a kit for immunodetection of target substances by using arterial blood, and belongs to the technical field of blood detection. The kit comprises at least: a first component containing luminescent microspheres combined with detection antibody 1 and a luminescent microsphere diluent, wherein the luminescent microsphere diluent contains 2-morpholinoethanesulfonic acid, KCl and NaHCO3; a second component containing biotin-labeled detection antibody 2 and a biotin diluent, wherein the biotin diluent contains dithiothreitol, CaCl2, adenine nucleoside and mouse anti-human hemoglobin antibody; and a third component containing photosensitive microspheres combined with streptomycin. The scheme can be used for immunodetection of target substances by using arterial blood, has excellent functional sensitivity and detection range, is not interfered by other substances in arterial blood, realizes rapid detection of all blood gas indexes and immunological indexes by using one tube of arterial blood, and has important significance for clinical blood detection.

The invention discloses grape pomace pretreatment liquid and a method for preparing microcrystalline cellulose from grape pomace. The grape pomace pretreatment liquid is prepared from laccase, polygalacturonase, acetosyringone, sodium dihydrogen phosphate, disodium hydrogen phosphate, dithiothreitol, fatty alcohol-polyoxyethylene ether, sorbitol and the balance of water. The method for preparing the microcrystalline cellulose from the grape pomace comprises the following steps: S1, cleaning the grape pomace with normal hexane, then cleaning with water, drying and crushing; s2, carrying out mixed reaction with the pretreatment liquid, and then separating out pretreatment residues; s3, adding a sodium carbonate solution for premixing, then adding a tetramethylammonium hydroxide solution and a sodium borohydride solution, and filtering after reaction to obtain filter residues; s4, adding a citric acid solution, adding carboxymethyl chitosan, adsorbing, and filtering to obtain cellulose residues; and S5, hydrolyzing and depolymerizing by using dilute acid, neutralizing, separating and washing an acidolysis product, and drying to obtain the microcrystalline cellulose. According to the method, impurity components which are difficult to treat in the grape pomace can be overcome, impurity removal and purification are completed, cellulose is reserved to the maximum extent, loss is reduced, and the yield is remarkably increased while high-purity microcrystalline cellulose is obtained.

Presented herein are methods for making, isolating, and purifying N-acetylcysteine amide, (2R,2R′)-3,3′-disulfanediyl bis(2-acetamidopropanamide, diNACA), intermediates and derivatives thereof comprising: alternatively contacting cystine with methanol and a chlorinating reagent to form an organic solution containing L-cystine dimethylester dihydrochloride; combining dried or undried L-cystine dimethylester dihydrochloride with a triethylamine, an acetic anhydride, and an acetonitrile to form a di-N-acetylcystine dimethylester; mixing dried di-N-acetylcystine dimethylester with ammonium hydroxide to form a di-N-acetylcystine amide (diNACA); and separating dried di-N-acetylcystine dimethylester into N-acetylcysteine amide with dithiothreitol, triethylamine, and an alcohol.

This invention belongs to the field of surgical wound repair technology, and provides a multifunctional composite hydrogel, its preparation method, and its applications. The invention involves mixing fibrinogen, mussel byssal protein, calcium chloride, methacrylated dextran (Dextran-MA), thrombin, dithiothreitol, and a dispersing solvent. The resulting mixture is then gelled to obtain a multifunctional composite hydrogel. Thrombin specifically promotes the cross-linking of fibrinogen to form a biomolecular network; dithiothreitol efficiently mediates the formation of an organic macromolecular network through a Michael addition reaction of Dextran-MA; the introduction of Mfp5, utilizing its abundant catechol groups to form multiple coordination and covalent cross-links with organic / biomolecular macromolecules, significantly enhances the interfacial adhesion strength of the multifunctional composite hydrogel; simultaneously maintaining the biocompatibility, biodegradability, hemostatic properties, anti-inflammatory effects, and healing-promoting capabilities of the multifunctional composite hydrogel.

The invention discloses a method for detecting a membranous nephropathy target antigen, which comprises the following steps: adding a protein extracting solution into a glomerular tissue to be detected, and incubating; adding a mixed enzyme of a LysC enzyme and a Trypsin enzyme, and incubating; adding a dithiothreitol solution, and incubating; adding an iodo-acetamide solution, and incubating at room temperature in a dark place; adding a precipitating agent, performing ultrasonic treatment and centrifugation, taking supernate, loading the supernate to a C18 small column, eluting the small column, and collecting eluent; freezing the eluent, blow-drying the eluent, and redissolving the eluent with a loading solution to obtain a test solution; and carrying out liquid chromatography-tandem mass spectrometry detection on the test solution. By adopting the detection method disclosed by the invention, the target antigen of membranous nephropathy can be accurately detected when the sampling volume is obviously reduced, so that the sampling difficulty is greatly reduced, and the application of the laser microdissection and liquid chromatography-mass spectrometry combined technology in the detection of the target antigen of membranous nephropathy is greatly promoted. The method is simple in process and short in detection time.

A composition for improving the detection performance of fluorescent quantitative PCR. The composition comprises bovine serum albumin, sorbitol, ammonium sulfate, formamide, tetramethylammonium chloride, and at least one of dithiothreitol and betaine. The present invention further relates to a qPCR reaction liquid containing the composition and a preparation method therefor. The composition can improve the sensitivity, specificity, and interference resistance of real-time fluorescent quantitative PCR.

The invention belongs to the technical field of agricultural biology, and discloses a non-tissue culture dependent transformation method for promoting effective transformation of potatoes by using growth factors. The invention discloses a reagent composition. The reagent composition comprises an infection solution and a bud inducing screening solution, wherein the infection liquid is prepared from acetosyringone, dithiothreitol and plant hormones; the bud inducing screening liquid comprises plant hormones, glufosinate-methyl and regeneration factors. The invention provides a reagent composition. The reagent composition can be used for non-tissue culture dependent genetic transformation of potatoes. By using the reagent composition, the non-tissue culture dependent genetic transformation step of the potatoes can be simplified, the conditions of chimera and false positive of transformants are reduced, and the transformation efficiency is remarkably improved.

The invention discloses application of a low-preference polymerase chain reaction buffer solution and a product, and relates to the technical field of molecular biology. The low-preference polymerase chain reaction buffer solution is prepared from 20 to 35 mM of Tris-HCl, 15 to 30 mM of K < + >, 1.5 to 2.2 mM of Mg < 2 + >, 0.6 to 1.0 M of betaine, 2.5 to 4.0 percent of dimethyl sulfoxide, 0.3 to 0.8 mM of ferrous sulfate heptahydrate, 0.2 to 0.6 mM of trehalose, 0.5 to 1.5 percent of NP-40, 0.2 to 0.5 mg / mL of recombinant escherichia coli single-chain binding protein and 0.5 to 1.0 mM of dithiothreitol; on the premise that the amplification yield is not reduced, the human whole genome GC preference coefficient is reduced to be smaller than or equal to 0.1, the coverage uniformity CV is smaller than or equal to 0.18, 0.1-50 ng of initial DNA is compatible, and the chromosome abnormality detection accuracy is improved.

This invention discloses a neutral dissociation agent and its preparation method for detecting the content of 25-hydroxyvitamin D in human blood samples, belonging to the field of biomedical detection technology. The dissociation agent is composed of disodium EDTA, sodium dodecyl sulfonate, dithiothreitol, hexadecyltrimethylammonium bromide, and hydroxypropyl-β-cyclodextrin dissolved in 50 mM tris(hydroxymethyl)aminomethane buffer, with the mass-volume ratios of each component being 0.5%-1%, 0.5%-1%, 0.2%-0.5%, 0.2%-0.5%, and 0.2%-0.5%, respectively. During preparation, each component is dissolved according to the steps, and the pH is adjusted to 7.4. This dissociation agent exhibits mild dissociation conditions, does not damage protein activity, has high dissociation efficiency, and is non-corrosive and non-toxic. The detection results show high specificity and sensitivity, with a coefficient of variation ≤6%. It can be directly integrated into automated detection processes and is suitable for large-scale clinical and research applications.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to an indometacin liniment for external use and a preparation process thereof, the indometacin liniment is prepared by compounding indometacin, a composite solubilizer, a composite stabilizer, a penetration enhancer, a humectant, a pH regulator and a solvent; wherein the composite solubilizer is a compound system of ethanol, N-sodium methyl taurate, lauroyl lysine and propylene glycol, the composite stabilizer is a compound system of ethylene diamine tetraacetic acid, acetylcysteine and dithiothreitol, the penetration enhancer is peppermint oil, the humectant is glycerol, the pH regulator is triethanolamine / citric acid, and the solvent is purified water. Through the specific compounding design of the components and the precise control of the preparation process, the obvious improvement of the water solubility of the indometacin and the effective guarantee of the content stability of the preparation are synchronously realized.

The application discloses a clinical biochemical composite quality control product, which comprises detection item raw materials, a matrix liquid and a protective agent; the detection item raw materials are alkaline phosphatase, amylase, sodium glycocholate, cholinesterase, creatinine, leucine aminopeptidase, lactate dehydrogenase, lipase, triglyceride, cholesterol, uric acid, urea, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, glucose, alpha-hydroxybutyric acid dehydrogenase, sodium hydrogen phosphate, magnesium chloride, calcium chloride, ferrous chloride, bilirubin, pancreatic amylase and sodium bicarbonate; the matrix liquid is bovine serum albumin and human serum albumin; and the protective agent is dithiothreitol (DTT), mannitol, trehalose, sodium chloride, a composite enzyme stabilizer AES and sodium azide. The quality control product can realize the detection of 27 clinical biochemical items, and the freeze-drying process is adopted, so that the freeze-dried composite quality control product has low viscosity, is easy to be re-dissolved and mixed, has high stability and has small matrix effect.

The invention discloses a multi-volume and multi-form STR (short tandem repeat) multiplex fluorescence multiplex amplification detection freeze-drying reagent as well as a preparation method and application thereof. The freeze-drying reagent consists of an STR multiplex fluorescence multiplex amplification system and a freeze-drying protective agent system, the STR multiplex fluorescent multiplex amplification system is composed of a 5X amplification buffer, a 5X primer mixture and Taq DNA polymerase; the 5X amplification buffer is prepared from Tris-HCl, ammonium sulfate, 20% Tween, magnesium chloride, bovine serum albumin, potassium chloride, ethylenediamine tetraacetic acid and dithiothreitol; the 5X primer mixture is a mixture of primers in a DNATperTM 30 kit of the Ministry of Public Security Identification Center; the freeze-drying protective agent system is composed of cane sugar, trehalose, polyethylene glycol 8000 and D-mannitol. The raw materials are freeze-dried in an in-situ freeze-drying or microsphere freeze-drying mode. According to the invention, the technical problems that the stability of the STR multiple fluorescent multiplex amplification detection freeze-drying reagent is poor and the detection accuracy is influenced by a freeze-drying protective agent due to the incoordination of an STR multiple fluorescent multiplex amplification system and a freeze-drying protection system can be solved.

The invention relates to the technical field of biological medicine, in particular to a preparation method and application of jujube extracellular vesicles. The preparation method comprises the following steps: preparing fresh jujubes into homogenate, adding PBS (Phosphate Buffer Solution), and carrying out multi-stage centrifugation to obtain a jujube extracellular vesicle crude extract; the jujube extracellular vesicle crude extract, paraformaldehyde and dithiothreitol are jointly incubated in PBS, then centrifugal filtration is conducted, and the jujube extracellular vesicles are obtained. The preparation method has the characteristics of stable process, low cost, good biological safety and the like, and has a wide application prospect in the field of medicines. The prepared jujube extracellular vesicles can be applied to anti-inflammatory drugs and immunoregulation drugs, and technical support is provided for development of novel natural-source drugs.

The invention discloses a preparation method of a nano-copper-based conductive composite material. The invention relates to the technical field of conductive composite materials, which comprises the following preparation steps: step 1, preparation of a poly (catechol-polyamine)-copper precursor suspension: S11, preparing a mixed monomer solution which comprises a catechol-polyamine system, 3, 4-dihydroxyphenylalanine, gallic acid and Tween-80; and S12, adjusting the pH value of the mixed monomer solution to 8.5-9.0 by adopting a Tris-HCl buffer solution, and then sequentially adding copper sulfate, a composite oxidation system, dithiothreitol and phytic acid into the mixed monomer solution. According to the preparation method, a catechol-polyamine system and 3, 4-dihydroxyphenylalanine gallic acid are subjected to synergistic polymerization, a poly (catechol-polyamine) coating layer with a steric hindrance effect is formed on the surfaces of copper ions, meanwhile, tween-80 optimizes the hydrophilicity and hydrophobicity of the surfaces of the particles, dithiothreitol regulates and controls charge distribution on the surfaces of the particles, and the copper ions can be effectively dispersed in the particles. And particle agglomeration is inhibited from the source through multiple effects.

The invention discloses a nucleic acid protection reagent composition and application thereof in a puncture sample nucleic acid extraction product, and belongs to the technical field of nucleic acid extraction. The technical problem to be solved is that an existing nucleic acid protective agent for nucleic acid extraction is complex in component and poor in safety. According to the key point of the technical scheme, the nucleic acid protection reagent composition is provided, and the nucleic acid protection reagent composition comprises 5-20 mM of dithiothreitol (DTT) and 5-20 mM of tris (2-carboxyethyl) phosphine (TCEP).

The invention provides a kit for detecting homocysteine and metabolites thereof in serum. The kit comprises a calibrator and a sample releasing agent, the calibrator comprises a component 1 and a component 2; the component 1 comprises a BSA (Bovine Serum Albumin) matrix and a first protective agent; the component 2 comprises homocysteine, metabolite of the homocysteine and a second protective agent; each of the first protective agent and the second protective agent is prepared from ascorbic acid, dithiothreitol, N-acetyl-DL-penicillamine and EDTA (Ethylene Diamine Tetraacetic Acid). According to the present invention, the 5-methyltetrahydrofolic acid and the homocysteine which are easily oxidized are well protected, and the synchronous detection of the folic acid, the 5-methyltetrahydrofolic acid, the vitamin B2, the pyridoxal phosphate, the methylmalonic acid, the homocysteine and the methionine is achieved. The kit has good stability at 4 DEG C and can meet the use requirement.

The application belongs to the technical field of genetic engineering, and relates to a Cas protein storage solution and application. The Cas protein storage solution comprises sucrose, a surfactant, dithiothreitol, glycerol and a bacteriostatic agent, wherein the mass-volume fraction of the sucrose is 1-10 %, the mass-volume fraction of the surfactant is 0.2-0.5 %, the mass-volume fraction of the dithiothreitol is 0.01-0.1 %, the mass-volume fraction of the glycerol is 30-60 %, and the mass-volume fraction of the bacteriostatic agent is 0.1-0.5 %. The storage solution provided by the application can obviously prolong the storage stability of Cas protein, and the preparation method is simple and low in cost.

The invention relates to the technical field of soil remediation, in particular to a heavy metal contaminated soil remediation agent and remediation method.The remediation agent is prepared from ferrous chloride tetrahydrate, butanediol, diethylene glycol, NaBH4, deionized water, tannic acid, a composite silane coupling agent and dithiothreitol. The invention further provides a heavy metal contaminated soil remediation method which is characterized by comprising the specific steps that firstly, organic matter compost and the heavy metal contaminated soil remediation agent are added into heavy metal contaminated soil, stirring and maintenance are conducted, and then the heavy metal contaminated soil remediation agent is obtained. The heavy metal contaminated soil remediation agent and the remediation method have the characteristics of low cost, simple method, high remediation effect, environmental friendliness and the like, and can adapt to various application environments.

This invention discloses a method for preparing a nano-copper-based conductive composite material, comprising the following preparation steps: Step 1, preparation of a poly(catechol-polyamine)-copper precursor suspension: S11, preparing a mixed monomer solution, wherein the mixed monomer solution contains a catechol-polyamine system, 3,4-dihydroxyphenylalanine, gallic acid, and Tween-80; S12, adjusting the pH of the mixed monomer solution to 8.5-9.0 using Tris-HCl buffer, and then sequentially adding copper sulfate, a composite oxidation system, dithiothreitol, and phytic acid. In this invention, through the synergistic polymerization of the catechol-polyamine system and 3,4-dihydroxyphenylalanine gallic acid, a poly(catechol-polyamine) coating layer with steric hindrance effect is formed on the surface of copper ions. Simultaneously, Tween-80 optimizes the hydrophilicity / hydrophobicity of the particle surface, and dithiothreitol regulates the charge distribution on the particle surface. These multiple effects fundamentally inhibit particle aggregation.

The application belongs to the technical field of biological medicine, and specifically discloses an ischemia modified albumin detection kit and method. The kit comprises reagent 1 and reagent 2; the reagent 1 comprises a water-soluble salt containing divalent cobalt ions and a surfactant, and has a pH of 7.1-8.5; the reagent 2 comprises dithiothreitol and a reducing protective agent, and has a pH of 4.0-7.5; the reducing protective agent is selected from at least one of tartaric acid, 2-hydroxy succinic acid, tris(2-carboxyethyl) phosphine, thiourea, mannitol and sodium sulfite. The kit is used to detect ischemia modified albumin by an albumin-cobalt combination test method, has the advantages of strong stability, high precision, good clinical correlation with mainstream manufacturers, wide linear range and the like, and can well meet the needs of clinical examination.

To provide a tree trunk injection agent having good stability of an active ingredient.SOLUTION: A tree injection containing an aqueous alcohol solution soluble compound such as acetic acid, hydroxyacetic acid, methacrylic acid, acrylic acid, oxalic acid, malonic acid, succinic acid, adipic acid, tartaric acid, diethyl tartrate, trans-aconitic acid, citric acid, gallic acid, phthalic acid, isophthalic acid, terephthalic acid, butanediol, trimethylolpropane, glycerol, dithiothreitol, dithioerythritol, sorbitol, maltitol, and reduced starch syrup, a monohydric alcohol having 1 to 4 carbon atoms such as methanol, ethanol, 1-propanol, 2-propanol, and 1-butanol, acetamiprid, and water.SELECTED DRAWING: None

This invention discloses a buffer solution for locked nucleic acid probe-based real-time quantitative PCR (RT-PCR). The buffer solution is composed of dithiothreitol, betaine, tetramethylammonium chloride, ammonium sulfate, tris(hydroxymethyl)aminomethane, magnesium chloride, a Tween-20 / glycerol mixture, and bovine serum albumin. The pH of the buffer solution is 7.0–9.0. The volume ratio of Tween-20 to glycerol in the Tween-20 / glycerol mixture is 1:80–120. By adding protective agents and promoters, adjusting the pH value, and adjusting the concentration of corresponding components, this buffer solution effectively protects the enzymes necessary for real-time quantitative PCR detection and improves the stability of modified probes in RTS. It is superior to other solutions for locked nucleic acid modified probes in RTS, improving detection sensitivity, fluorescence intensity, and result reliability. Therefore, this buffer solution has broad application prospects in detections requiring more stringent and precise data.

The application discloses a kind of digestive juice for separating gastric corpus stem cell and its use method, belong to stem cell separation technical field;The digestive juice includes the following concentration components:18.5~28 nM Na2HPO4, 40~48 nM KH2PO4, 460.5~530 nM NaCl, 1~15.2 nM KCl, 200~350.55 nM D-furan fructosyl-D-glucopyranoside, 400~605 nM D-glucitol, 2~4 nM ethylenediaminetetraacetic acid disodium and 2~3 nM 1,4-dithiothreitol.The application also discloses the use method of the above digestive juice.The digestive juice for separating gastric corpus stem cell and its use method of the application can solve the problems of low cell yield, low activity, insufficient purity and poor repeatability of existing digestive juice and supporting use method, and have wide application prospect.