87 results about "Dithiothreitol" patented technology

The invention provides an additive composition containing a bioactive enzyme and a preparation method of the additive composition, and belongs to the technical field of biological enzymes. The preparation method comprises the following steps: fixing phytic acid and cyclodextrin on hydroxyapatite, modifying with tannic acid, reacting with dithiothreitol modified lysozyme to prepare an immobilized compound, adding 2, 2-dimethoxy-2-phenylacetophenone and methyl methacrylate, and carrying out irradiation reaction with an ultraviolet lamp to prepare the additive composition containing the bioactive enzyme. The broad-spectrum antibacterial property and the antiviral property of the lysozyme are greatly improved, the sterilization effect on gram negative bacteria is obviously improved, meanwhile, the stability of enzyme is greatly improved, the enzyme is not prone to inactivation, the preservation time of the enzyme is prolonged, the application effect of the enzyme is widened, meanwhile, the lysozyme has good killing capacity on oral bacteria and viruses, the addition of preservatives is reduced, and the cost is reduced. The cleaning effect and stability of the product are improved, tooth demineralization can be prevented, decayed teeth can be prevented, and wide application prospects are achieved.

The invention discloses self-repairing waterborne polyurethane based on double dynamic bond synergy and a preparation method thereof.The method comprises the following steps that 1, in the nitrogen atmosphere, after isophorone diisocyanate, polytetrahydrofuran diol and dimethylolpropionic acid react for a period of time, a catalyst is added for continuous reaction, and a prepolymer is synthesized; (2) adding N, N-di-tert-butyl ethylenediamine into the prepolymer, and carrying out chain extension reaction; (3) adding dithiothreitol into the solution after the reaction in the step (2) to carry out cross-linking reaction; and (4) adding triethylamine into the solution obtained after the reaction in the step (3), and carrying out a salt forming reaction to obtain the self-repairing waterborne polyurethane emulsion based on double dynamic bond synergy. High self-healing efficiency and mechanical performance are achieved by introducing dynamic covalent bonds with double thermal responses, and reprocessing and recycling are achieved at the same time.

The invention belongs to the technical field of biomedical materials and regenerative medicine, and relates to collagen-based injectable fiber hydrogel as well as a preparation method and application thereof. According to the injectable fiber hydrogel, methacrylated collagen and dithiothreitol are subjected to a thiol-ene click reaction rapidly under the action of a photoinitiator through illumination, a primary cross-linked network is formed, and the injection and in-situ gel forming requirements are met; then under the conditions that the pH is 6.5-8.0 and the temperature is 30-39 DEG C, collagen molecules are self-assembled into a nanofiber structure, and a bionic network similar to a natural extracellular matrix is obtained. The hydrogel disclosed by the invention has the advantages of rapid gelation, structural biomimetic property and biological activity; residual sulfydryl endows the hydrogel with active oxygen scavenging capacity, so that oxidative stress can be relieved, and immune response can be regulated; the fibrotic structure promotes cell adhesion, migration and tissue regeneration.

The invention relates to a DNA (deoxyribonucleic acid) ligation buffer solution and application thereof, the DNA ligation buffer solution comprises 5-15 mM of divalent metal ions, 0.5-5 mM of DTT (dithiothreitol), 10-15% of polyethylene glycol, 0.5-5 mM of ATP (adenosine triphosphate) and a Tris-HCl buffer solution, and the pH (potential of hydrogen) value of the Tris-HCl buffer solution is 7-9. The DNA ligation buffer solution can significantly improve the ligation reaction efficiency, and increase ligation products in the ligation reaction and the number of molecules capable of being sequenced during library construction.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of an endoplasmic reticulum protein antioxidant (reducing agent) in prevention and treatment of bombyx mori nuclear polyhedrosis virus. A screening test finds that the related endoplasmic reticulum protein antioxidants beta-mercaptoethanol (BME), dithiothreitol (DTT) and / or tris (2-carboxyethyl) phosphine (TCEP) have the effect of inhibiting the bombyx mori nuclear polyhedrosis virus (BmNPV), and the antioxidants interfere the replication and proliferation of the virus by destroying disulfide bonds in key proteins of the BmNPV, so that the bombyx mori nuclear polyhedrosis virus can be inhibited. The stability and activity of virions are directly influenced, so that the infection of the viruses on the silkworms is inhibited, a brand new thought is provided for the development of antiviral drugs of the silkworms, and the application value is good.

The invention discloses a bovine methane emission reduction method for intragastric delivery of targeted edition methanosarcina, and belongs to the technical field of biology. The method comprises the following steps: S1, preparing a conversion mixed solution: 50 mM of a PIPES buffer solution, 0.35 M of sucrose, 0.6 M of sodium chloride, 1 mM of magnesium chloride, 0.00001% of resazurin, 0.05% of cysteine hydrochloride, 1 mM of dithiothreitol, 40% of PEG4000 and 6 ml of PLL-PEG-DNA nanoparticles; s2, delivering the converted mixed solution into a ruminant rumen environment through intragastric administration; under the action of a conversion mixed solution taking polyethylene glycol 4000 as a main component, the gene editing system is delivered into methanosarcina, so that methanosarcina is modified, the number of methanosarcina in a bovine rumen is reduced, and the emission of bovine methane gas is further reduced.

The application discloses a marine-derived cholesterol oxidase and a preparation method and application thereof, and relates to the technical field of biology. The amino acid sequence of the marine-derived cholesterol oxidase (SkChOx) is shown in SEQ ID NO. 1 or 3, the specific enzyme activity of the marine-derived cholesterol oxidase can reach 31.9 U / mg, the marine-derived cholesterol oxidase has excellent anti-reverse performance, maintains high stability in the pH range of 5.0-10.0, has good temperature tolerance, organic solvent tolerance and metal ion tolerance, and exhibits unique salt activation effect and salt tolerance. The enzyme is compounded with dithiothreitol (DTT) and nonylphenol polyoxyethylene ether (NP-40) to form a liquid enzyme preparation, and after incubation at 37 DEG C for 7 days under accelerated conditions, the catalytic activity of the liquid enzyme preparation still remains about 87%, the liquid enzyme preparation has excellent long-acting storage stability, and has wide application prospects in the fields of clinical diagnosis, food physical and chemical inspection, biosensor development and industrial preparation of steroid drugs.

The invention discloses a rolling circle amplification (RCA) molecular beacon based on DNA synthesized silver nanoclusters (AgNCs / DNA) and application of the rolling circle amplification (RCA) molecular beacon in DNA and RNA virus detection. According to the invention, a padlock template probe PlP with a specific secondary structure is used for carrying out two-enzyme one-step method RCA to generate single-stranded DNA containing a G-rich sequence tandem repeat element. Then H2O2 is added to eliminate the influence of residual dithiothreitol DTT in the RCA reaction, and then AgNCs / DT-GSP-t4 is used as a signal probe to generate a specific fluorescence enhancement signal for a to-be-detected target under the action of a G-rich proximity fluorescence enhancement effect. The RCA reaction does not need primer mediation, so that PlP design and RCA operation steps are simplified; meanwhile, the single-PlP or double-PlP probe is combined with the fluorescent molecular beacon, so that single-base mismatch at a PlP connection point can be recognized, and the probe has the advantage of high specificity.

The present application discloses a method for increasing the oil accumulation in plants, aiming to solve the problem that there is a lack of new effective technical approaches for improving oil accumulation in current plants. The present application has found through research that endoplasmic reticulum stress induced by regulating chemical substances (tunicamycin, dithiothreitol, thapsigargin) or gene ( lew1 gene or its homologous gene) mutation can increase the oil content in plant tissues and seeds; increasing the oil accumulation in plants can effectively increase the oil content of oil crops or other plants, effectively increase the oil production of plants, and is of great significance to the production of vegetable oil.

The invention relates to the technical field of nucleic acid detection, in particular to a lysis buffer solution, a kit and an extraction method for extracting free DNA of peripheral blood. The lysis buffer solution for extracting the free DNA of the peripheral blood comprises a first lysis solution and a second lysis solution, the first lysis solution takes guanidine hydrochloride as a main chaotropic agent and at least one of ammonium thiocyanate and guanidine isothiocyanate as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate and ascorbic acid; the second lysis solution takes guanidine hydrochloride as a main chaotropic agent and urea as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate, ascorbic acid and polyethylene glycol. The buffer solution of the lysate does not contain SDS (sodium dodecyl sulfate), so that the residue of SDS in an extracted product can be avoided, and the amount of extracted free DNA (deoxyribonucleic acid) is more.

The invention provides a method for improving the purity of collagen in the fermentation process of engineering bacteria and application of the method, and relates to the technical field of fermentation. Dithiothreitol (DTT) is used as an exogenous additive for the first time to be applied to the process of producing collagen through strain fermentation, and it is found that DTT can relieve intracellular stress of yeast cells and effectively relieve the collagen degradation condition in the fermentation process, and a fermentation product with higher purity is obtained. The invention provides a novel method for producing collagen through fermentation of engineering bacteria, and the method is simple and easy to operate, can effectively improve the purity of a fermentation product, and has a wide application prospect.

The invention provides a mycobacterium tuberculosis rapid detection system based on digital CRISPR-Cas polydispersion liquid drops, the rapid detection system does not comprise a PCR amplification detection device, the system comprises a nucleic acid extraction subsystem, the nucleic acid extraction subsystem is used for heating a mycobacterium tuberculosis sample to be detected by using a PBS buffer solution containing dithiothreitol, and the nucleic acid extraction subsystem is used for extracting nucleic acid from the mycobacterium tuberculosis sample to be detected. Extracting mycobacterium tuberculosis DNA in a sample to be detected; a polydispersion liquid drop generation subsystem and a polydispersion liquid drop detection subsystem. The rapid detection system disclosed by the invention realizes on-site amplification-free detection of tubercle bacillus by combining rapid extraction of tubercle bacillus nucleic acid and a digital CRISPR technology.

This invention belongs to the field of rust-preventive coating material preparation technology, specifically relating to a water-based self-healing rust-preventive coating and its preparation method. First, an unsaturated ester, a fluorinated unsaturated ester, and 1,4-dithiothreitol are prepared into a fluorinated hydrophilic polymer under the conditions of an initiator, a chain extender, and water as a solvent. Then, the obtained fluorinated hydrophilic polymer is reacted in an aqueous phase to prepare a water-based fluorinated acrylic copolymer. Finally, the water-based fluorinated acrylic copolymer is compounded with modified CeO2 nanoparticles, iron oxide, talc, and antioxidant 168 to obtain a water-based self-healing coating material. This invention achieves self-healing of the coating material by introducing a D-A reaction, and uses water as a diluent, is non-toxic, odorless, harmless to humans, and does not pollute the environment. By introducing cerium groups, this coating improves the corrosion resistance of metals, greatly extending the service life of the material and possessing high application value.

The invention discloses a mussel byssus gland mucoprotein subunit and a preparation method and application thereof.The preparation method comprises the steps that a mussel byssus gland serves as a raw material, the outer layer of the byssus gland is removed through neutral protease hydrolysis, then the secondary outer layer of the byssus gland is removed through pepsase hydrolysis, a byssus gland core is obtained, a urea solution is added to the byssus gland core for homogenization treatment, and the mussel byssus gland mucoprotein subunit is obtained; the preparation method comprises the following steps: centrifuging to remove insoluble impurities, adding dithiothreitol into supernate to obtain crude extract of the byssus mucoprotein, performing microfiltration to remove biological impurities, performing nanofiltration on microfiltrate to obtain byssus mucoprotein subunit liquid, and performing spray drying or freeze drying on collected liquid to obtain high-solubility mussel byssus mucoprotein subunit powder. The high-solubility mussel byssus mucoprotein subunit is obtained through a double-enzyme urea nanofiltration engineering reduction method, has the effects of thickening a skin basal layer, promoting hair growth, inducing skin regeneration and the like, and is applied to the field of skin beauty treatment and the field of biological medical instruments.

The present invention relates to the technical field of polymer synthesis and relates to a preparation method for an oil-proofing agent based on polymerization of acrylic acid and organosilicon. In the present invention, the method comprises: adding methyl acrylate, hydroxyethyl acrylate, dithiothreitol, m-trifluoromethylcinnamaldehyde, and triethylamine to a reaction kettle, and stirring and heating the mixture for a reaction; adding an initiator, and continuing the reaction, so that the acrylic monomers undergo a polymerization reaction to form a polymer having good stability and mobility; further adding α,ω-dihydroxypolydimethylsiloxane and continuing the reaction, so that the linear organosilicon and the polymer undergo a polymerization reaction; adding an emulsifier, and stirring and mixing the mixture for a reaction to obtain an oil-proofing agent; and adding the oil-proofing agent to a pulp for food boxes and uniformly stirring the mixture, so that the polymer is uniformly distributed on the surface of the pulp for food boxes to form a dense oil-proofing film, thereby effectively preventing the adhesion of oil stains. The oil-proofing agent prepared in the present invention has good oil-proofing performance, and can effectively prevent the adhesion of oil stains, thereby maintaining the cleanliness of food packaging and catering utensils.

This invention relates to a triboelectric nanogenerator and its preparation method, belonging to the technical field of triboelectric nanogenerators. The preparation method includes the following steps: S1, dispersing styrene-ethylene-butene-styrene block copolymer, polyvinylidene fluoride, and MXene nanomaterials in a mixed solvent to obtain a negative friction layer spinning solution; S2, using dynamic pattern-induced multi-material nanofiber electrospinning technology, forming a negative friction layer from the negative friction layer spinning solution; S3, dispersing thermoplastic starch, sodium carboxymethyl cellulose, plasticizer, bis(2-hydroxyethyl) disulfide, and dithiothreitol in water to obtain a positive friction layer film-forming solution; S4, forming a positive friction layer from the positive friction layer film-forming solution; S5, bonding the micro-nano structures of the positive and negative friction layers face-to-face to obtain the triboelectric nanogenerator. This generator combines high output, long lifespan, and flexibility, and can be adapted to diverse scenarios such as wearable electronics, industrial high-temperature sensing, and environmental mechanical energy harvesting.

The invention provides a low-temperature rapid bottle washing process for a trademark coated with UV gloss oil and a composite label-removing auxiliary agent, and the process comprises the following steps: 1, raising the temperature of alkali liquor of a bottle washing machine to 60 DEG C, adding sodium carbonate, sodium silicate and alkyl glycoside (APG) in the composite label-removing auxiliary agent, and stirring until the sodium carbonate, the sodium silicate and the alkyl glycoside (APG) are completely dissolved; 2, heating to 80 DEG C, adding sodium gluconate and trisodium citrate, and continuously stirring for dissolving; 3, further heating to 88-90 DEG C, adding EDTA (Ethylene Diamine Tetraacetic Acid), DTT (Dithiothreitol) and urea, keeping constant temperature, uniformly mixing, and feeding into a bottle for production; 4, bottle washing process parameters are as follows: the concentration of the alkali liquor is 2.8-3.2%, the temperature is 88-90 DEG C, the addition amount of the composite label removing auxiliary agent is 2.0-2.5%, and the bottle washing speed is 2.8-3.0 million bottles per hour. Compared with the prior art, the method has the beneficial effects that efficient label removing and cleaning effects are achieved, meanwhile, energy consumption is reduced through low-temperature operation, the risk of high-temperature alkali liquor splashing during working is avoided, and the service life of the device is prolonged. The accident rate of scalding workers is reduced, and the production safety is improved.

The invention relates to the technical field of collagen material degradation, and particularly discloses an in-vitro degradation method of a collagen biological material, which comprises the following steps: pretreating the collagen biological material to obtain a to-be-degraded sample; adding the enzymatic hydrolysate into a to-be-degraded sample, sealing, and degrading at 37 + / -2 DEG C to obtain a degradation solution; the enzymatic hydrolysate is prepared from the following components: 10 to 20 mg / L of collagenase, 3 to 6 mg / L of sorbitol, 1 to 2 mg / L of calcium chloride, 0.3 to 0.8 mg / L of dithiothreitol and the balance of a Tris-HCl buffer solution with the pH value of 7 to 8. The in-vitro degradation method of the collagen biological material provided by the invention has the advantages of good degradation stability, small batch-to-batch difference and the like, and can be used for investigating the degradation behavior of the collagen biological medical device against collagenase in vivo, so that research and development and quality control of the collagen biological medical device are realized.

This invention discloses a rapid detection kit for HAP / VAP pathogens and drug resistance genes, and its applications, belonging to the field of biomedical detection technology. Addressing the problems of long detection processes, complex operations, and poor sample adaptability in existing tNGS technology, this invention provides an optimized detection kit. The kit includes: a sample pretreatment and nucleic acid extraction reagent system, whose sample preservation solution contains 5% dithiothreitol and 2.5% potassium azide for simultaneous sample liquefaction and salt ion chelation during transportation; and an amplification and library construction reagent system, which contains a primer mixture consisting of 175 primer pairs, E. coli synthase III, and separately added magnesium ions. By integrating the above reagent systems and optimizing the entire process, this invention reduces the total time from sample pretreatment to report generation to less than 2.5 hours. This invention has the advantages of extremely fast detection speed, wide sample applicability, high operational stability, and ease of automation.

The application discloses a method and a kit for immunodetection of target substances by using arterial blood, and belongs to the technical field of blood detection. The kit comprises at least: a first component containing luminescent microspheres combined with detection antibody 1 and a luminescent microsphere diluent, wherein the luminescent microsphere diluent contains 2-morpholinoethanesulfonic acid, KCl and NaHCO3; a second component containing biotin-labeled detection antibody 2 and a biotin diluent, wherein the biotin diluent contains dithiothreitol, CaCl2, adenine nucleoside and mouse anti-human hemoglobin antibody; and a third component containing photosensitive microspheres combined with streptomycin. The scheme can be used for immunodetection of target substances by using arterial blood, has excellent functional sensitivity and detection range, is not interfered by other substances in arterial blood, realizes rapid detection of all blood gas indexes and immunological indexes by using one tube of arterial blood, and has important significance for clinical blood detection.

The invention discloses a multi-target synergistic anti-wrinkle firming beauty makeup composition as well as a preparation method and application of the multi-target synergistic anti-wrinkle firming beauty makeup composition. The technical defects that an existing polypeptide product is single in effect, low in permeability and poor in stability are effectively overcome. The composition takes acetyl hexapeptide-8, palmitoyl tripeptide-1 and decapeptide-4 as core active components, is compounded with auxiliary materials such as a humectant, a penetration enhancer, a stabilizer and a preservative, and is prepared by compounding according to a specific mass percent. The action mechanisms of'inhibiting dynamic wrinkles, promoting collagen synthesis and protecting a collagen structure 'of the three polypeptides are complementary, so that multi-target synergistic anti-wrinkle is realized; the skin permeability is improved by means of plant-derived ceramide NP, and the polypeptide stability is enhanced through dithiothreitol and pH regulation. The invention can be widely applied to beauty makeup products such as essence, face cream, eye cream and the like.

The present invention relates to the field of biotechnology and specifically discloses a method for transforming Rhodosporidium toruloides. The method comprises the following steps: 1) obtaining a single clone of Rhodosporidium toruloides, inoculating it into a culture medium for cultivation, and collecting the cells; 2) adding a transformation solution to the cells in step 1), resuspending the cells, and allowing them to stand; the transformation solution comprises PEG, a mediator, and salmon sperm DNA; 3) adding the DNA to be transformed to the cells in step 2), and incubating the cells; 4) adding a dithiothreitol solution or a dimethyl sulfoxide solution to the cell solution in step 3), followed by heat shock, resuspending the collected cells, and incubating them. The present invention constructs an efficient and reliable method for transforming Rhodosporidium toruloides with exogenous DNA. The transformation method of the present invention for Rhodosporidium toruloides has a simple process and high transformation efficiency, with a maximum transformation efficiency of 1938 transformants / μg DNA.

The invention discloses grape pomace pretreatment liquid and a method for preparing microcrystalline cellulose from grape pomace. The grape pomace pretreatment liquid is prepared from laccase, polygalacturonase, acetosyringone, sodium dihydrogen phosphate, disodium hydrogen phosphate, dithiothreitol, fatty alcohol-polyoxyethylene ether, sorbitol and the balance of water. The method for preparing the microcrystalline cellulose from the grape pomace comprises the following steps: S1, cleaning the grape pomace with normal hexane, then cleaning with water, drying and crushing; s2, carrying out mixed reaction with the pretreatment liquid, and then separating out pretreatment residues; s3, adding a sodium carbonate solution for premixing, then adding a tetramethylammonium hydroxide solution and a sodium borohydride solution, and filtering after reaction to obtain filter residues; s4, adding a citric acid solution, adding carboxymethyl chitosan, adsorbing, and filtering to obtain cellulose residues; and S5, hydrolyzing and depolymerizing by using dilute acid, neutralizing, separating and washing an acidolysis product, and drying to obtain the microcrystalline cellulose. According to the method, impurity components which are difficult to treat in the grape pomace can be overcome, impurity removal and purification are completed, cellulose is reserved to the maximum extent, loss is reduced, and the yield is remarkably increased while high-purity microcrystalline cellulose is obtained.

Presented herein are methods for making, isolating, and purifying N-acetylcysteine amide, (2R,2R′)-3,3′-disulfanediyl bis(2-acetamidopropanamide, diNACA), intermediates and derivatives thereof comprising: alternatively contacting cystine with methanol and a chlorinating reagent to form an organic solution containing L-cystine dimethylester dihydrochloride; combining dried or undried L-cystine dimethylester dihydrochloride with a triethylamine, an acetic anhydride, and an acetonitrile to form a di-N-acetylcystine dimethylester; mixing dried di-N-acetylcystine dimethylester with ammonium hydroxide to form a di-N-acetylcystine amide (diNACA); and separating dried di-N-acetylcystine dimethylester into N-acetylcysteine amide with dithiothreitol, triethylamine, and an alcohol.

The invention belongs to the technical field of enzyme activity determination, and particularly relates to a method for determining nitrate reductase, glutamate synthetase and glutamate dehydrogenase, which comprises the following steps of: improving an extracting solution of a nitrogen metabolism enzyme, taking a Tris buffer solution containing MgSO4, EGTA and dithiothreitol as a crude enzyme extracting solution, and extracting the crude enzyme to obtain the nitrate reductase, the glutamate synthetase and the glutamate dehydrogenase. The determination method of nitrate reductase, glutamate synthase and glutamate dehydrogenase is further improved, and the method is simple to operate, time-saving and suitable for large-scale determination while the accuracy is ensured.

The present invention provides a kind of kit and its use for detecting psychotropic drugs in hair, the kit includes hair cleanser and hair digester, the hair cleanser includes Tween-20 and TXR-1, and the hair digester includes dithiothreitol, tris (2-carboxyethyl) phosphine hydrochloride and CHAPS. The hair sample is pre-treated with the kit provided by the present invention, and the pollutants attached to the hair sample can be effectively removed, the operating steps are simplified, the accuracy and stability of the test results are improved, and the sample extract can still be accurately detected after being stored for a certain period of time. In addition, the psychotropic drugs in the sample extract are detected in combination with quantum dot immunofluorescence chromatography, and a variety of new psychotropic drugs can be fully extracted and accurately detected, which greatly improves the sensitivity and specificity of the detection method, and is conducive to rapid screening on site.

The invention relates to a hair lysate and application thereof. The hair lysate is prepared from the following components in percentage by volume: 0.05 to 1.0 percent of ethyl phenyl polyethylene glycol, 2 to 18 mg / mL of dithiothreitol and 2 to 20 U / mL of protease. The NP-40 is a surfactant, the dithiothreitol is a reducing agent, and the NP-40 and the dithiothreitol jointly act with the protease, so that drug components possibly existing in the hair can be quickly released, ultrasonic or freezing grinding is not needed, and the operation is simple and convenient. The hair lysate can meet the requirement of qualitative detection only after being treated for 10 minutes, the best effect can be obtained if the treatment time is as long as 30 minutes, the release efficiency is superior to that of a traditional method, and whether a hair sample contains trace drugs or not can be accurately detected by matching with subsequent liquid chromatography-tandem mass spectrometry analysis.

This invention belongs to the field of surgical wound repair technology, and provides a multifunctional composite hydrogel, its preparation method, and its applications. The invention involves mixing fibrinogen, mussel byssal protein, calcium chloride, methacrylated dextran (Dextran-MA), thrombin, dithiothreitol, and a dispersing solvent. The resulting mixture is then gelled to obtain a multifunctional composite hydrogel. Thrombin specifically promotes the cross-linking of fibrinogen to form a biomolecular network; dithiothreitol efficiently mediates the formation of an organic macromolecular network through a Michael addition reaction of Dextran-MA; the introduction of Mfp5, utilizing its abundant catechol groups to form multiple coordination and covalent cross-links with organic / biomolecular macromolecules, significantly enhances the interfacial adhesion strength of the multifunctional composite hydrogel; simultaneously maintaining the biocompatibility, biodegradability, hemostatic properties, anti-inflammatory effects, and healing-promoting capabilities of the multifunctional composite hydrogel.

The present invention discloses a mussel foot gland mucin subunit and its preparation method and application, including: using mussel foot gland as raw material, first removing the outer layer of the foot gland by hydrolysis with neutral protease, then removing the secondary outer layer of the foot gland by hydrolysis with pepsin to obtain the foot gland core, adding urea solution to the foot gland core for homogenization, centrifuging to remove insoluble impurities, adding dithiothreitol to the supernatant to obtain a crude extract of foot gland mucin, microfiltration to remove biological impurities and nanofiltration to obtain foot gland mucin subunit liquid, and spray drying or freeze drying the collected liquid to obtain a highly soluble mussel foot gland mucin subunit powder. The present invention obtains highly soluble mussel foot gland mucin subunits by a dual-enzyme urea nanofiltration engineering reduction method, which has the effects of thickening the basal layer of the skin, promoting hair growth, inducing skin regeneration, etc., and is applied to the field of skin medical beauty and the field of biomedical devices.