59 results about "Dithiothreitol" patented technology

The invention belongs to the technical field of biomedical materials and regenerative medicine, and relates to collagen-based injectable fiber hydrogel as well as a preparation method and application thereof. According to the injectable fiber hydrogel, methacrylated collagen and dithiothreitol are subjected to a thiol-ene click reaction rapidly under the action of a photoinitiator through illumination, a primary cross-linked network is formed, and the injection and in-situ gel forming requirements are met; then under the conditions that the pH is 6.5-8.0 and the temperature is 30-39 DEG C, collagen molecules are self-assembled into a nanofiber structure, and a bionic network similar to a natural extracellular matrix is obtained. The hydrogel disclosed by the invention has the advantages of rapid gelation, structural biomimetic property and biological activity; residual sulfydryl endows the hydrogel with active oxygen scavenging capacity, so that oxidative stress can be relieved, and immune response can be regulated; the fibrotic structure promotes cell adhesion, migration and tissue regeneration.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of an endoplasmic reticulum protein antioxidant (reducing agent) in prevention and treatment of bombyx mori nuclear polyhedrosis virus. A screening test finds that the related endoplasmic reticulum protein antioxidants beta-mercaptoethanol (BME), dithiothreitol (DTT) and / or tris (2-carboxyethyl) phosphine (TCEP) have the effect of inhibiting the bombyx mori nuclear polyhedrosis virus (BmNPV), and the antioxidants interfere the replication and proliferation of the virus by destroying disulfide bonds in key proteins of the BmNPV, so that the bombyx mori nuclear polyhedrosis virus can be inhibited. The stability and activity of virions are directly influenced, so that the infection of the viruses on the silkworms is inhibited, a brand new thought is provided for the development of antiviral drugs of the silkworms, and the application value is good.

The application discloses a marine-derived cholesterol oxidase and a preparation method and application thereof, and relates to the technical field of biology. The amino acid sequence of the marine-derived cholesterol oxidase (SkChOx) is shown in SEQ ID NO. 1 or 3, the specific enzyme activity of the marine-derived cholesterol oxidase can reach 31.9 U / mg, the marine-derived cholesterol oxidase has excellent anti-reverse performance, maintains high stability in the pH range of 5.0-10.0, has good temperature tolerance, organic solvent tolerance and metal ion tolerance, and exhibits unique salt activation effect and salt tolerance. The enzyme is compounded with dithiothreitol (DTT) and nonylphenol polyoxyethylene ether (NP-40) to form a liquid enzyme preparation, and after incubation at 37 DEG C for 7 days under accelerated conditions, the catalytic activity of the liquid enzyme preparation still remains about 87%, the liquid enzyme preparation has excellent long-acting storage stability, and has wide application prospects in the fields of clinical diagnosis, food physical and chemical inspection, biosensor development and industrial preparation of steroid drugs.

The invention relates to the technical field of nucleic acid detection, in particular to a lysis buffer solution, a kit and an extraction method for extracting free DNA of peripheral blood. The lysis buffer solution for extracting the free DNA of the peripheral blood comprises a first lysis solution and a second lysis solution, the first lysis solution takes guanidine hydrochloride as a main chaotropic agent and at least one of ammonium thiocyanate and guanidine isothiocyanate as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate and ascorbic acid; the second lysis solution takes guanidine hydrochloride as a main chaotropic agent and urea as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate, ascorbic acid and polyethylene glycol. The buffer solution of the lysate does not contain SDS (sodium dodecyl sulfate), so that the residue of SDS in an extracted product can be avoided, and the amount of extracted free DNA (deoxyribonucleic acid) is more.

The invention provides a method for improving the purity of collagen in the fermentation process of engineering bacteria and application of the method, and relates to the technical field of fermentation. Dithiothreitol (DTT) is used as an exogenous additive for the first time to be applied to the process of producing collagen through strain fermentation, and it is found that DTT can relieve intracellular stress of yeast cells and effectively relieve the collagen degradation condition in the fermentation process, and a fermentation product with higher purity is obtained. The invention provides a novel method for producing collagen through fermentation of engineering bacteria, and the method is simple and easy to operate, can effectively improve the purity of a fermentation product, and has a wide application prospect.

This invention belongs to the field of rust-preventive coating material preparation technology, specifically relating to a water-based self-healing rust-preventive coating and its preparation method. First, an unsaturated ester, a fluorinated unsaturated ester, and 1,4-dithiothreitol are prepared into a fluorinated hydrophilic polymer under the conditions of an initiator, a chain extender, and water as a solvent. Then, the obtained fluorinated hydrophilic polymer is reacted in an aqueous phase to prepare a water-based fluorinated acrylic copolymer. Finally, the water-based fluorinated acrylic copolymer is compounded with modified CeO2 nanoparticles, iron oxide, talc, and antioxidant 168 to obtain a water-based self-healing coating material. This invention achieves self-healing of the coating material by introducing a D-A reaction, and uses water as a diluent, is non-toxic, odorless, harmless to humans, and does not pollute the environment. By introducing cerium groups, this coating improves the corrosion resistance of metals, greatly extending the service life of the material and possessing high application value.

The present invention relates to the technical field of polymer synthesis and relates to a preparation method for an oil-proofing agent based on polymerization of acrylic acid and organosilicon. In the present invention, the method comprises: adding methyl acrylate, hydroxyethyl acrylate, dithiothreitol, m-trifluoromethylcinnamaldehyde, and triethylamine to a reaction kettle, and stirring and heating the mixture for a reaction; adding an initiator, and continuing the reaction, so that the acrylic monomers undergo a polymerization reaction to form a polymer having good stability and mobility; further adding α,ω-dihydroxypolydimethylsiloxane and continuing the reaction, so that the linear organosilicon and the polymer undergo a polymerization reaction; adding an emulsifier, and stirring and mixing the mixture for a reaction to obtain an oil-proofing agent; and adding the oil-proofing agent to a pulp for food boxes and uniformly stirring the mixture, so that the polymer is uniformly distributed on the surface of the pulp for food boxes to form a dense oil-proofing film, thereby effectively preventing the adhesion of oil stains. The oil-proofing agent prepared in the present invention has good oil-proofing performance, and can effectively prevent the adhesion of oil stains, thereby maintaining the cleanliness of food packaging and catering utensils.

This invention relates to a triboelectric nanogenerator and its preparation method, belonging to the technical field of triboelectric nanogenerators. The preparation method includes the following steps: S1, dispersing styrene-ethylene-butene-styrene block copolymer, polyvinylidene fluoride, and MXene nanomaterials in a mixed solvent to obtain a negative friction layer spinning solution; S2, using dynamic pattern-induced multi-material nanofiber electrospinning technology, forming a negative friction layer from the negative friction layer spinning solution; S3, dispersing thermoplastic starch, sodium carboxymethyl cellulose, plasticizer, bis(2-hydroxyethyl) disulfide, and dithiothreitol in water to obtain a positive friction layer film-forming solution; S4, forming a positive friction layer from the positive friction layer film-forming solution; S5, bonding the micro-nano structures of the positive and negative friction layers face-to-face to obtain the triboelectric nanogenerator. This generator combines high output, long lifespan, and flexibility, and can be adapted to diverse scenarios such as wearable electronics, industrial high-temperature sensing, and environmental mechanical energy harvesting.

The invention relates to the technical field of collagen material degradation, and particularly discloses an in-vitro degradation method of a collagen biological material, which comprises the following steps: pretreating the collagen biological material to obtain a to-be-degraded sample; adding the enzymatic hydrolysate into a to-be-degraded sample, sealing, and degrading at 37 + / -2 DEG C to obtain a degradation solution; the enzymatic hydrolysate is prepared from the following components: 10 to 20 mg / L of collagenase, 3 to 6 mg / L of sorbitol, 1 to 2 mg / L of calcium chloride, 0.3 to 0.8 mg / L of dithiothreitol and the balance of a Tris-HCl buffer solution with the pH value of 7 to 8. The in-vitro degradation method of the collagen biological material provided by the invention has the advantages of good degradation stability, small batch-to-batch difference and the like, and can be used for investigating the degradation behavior of the collagen biological medical device against collagenase in vivo, so that research and development and quality control of the collagen biological medical device are realized.

This invention discloses a rapid detection kit for HAP / VAP pathogens and drug resistance genes, and its applications, belonging to the field of biomedical detection technology. Addressing the problems of long detection processes, complex operations, and poor sample adaptability in existing tNGS technology, this invention provides an optimized detection kit. The kit includes: a sample pretreatment and nucleic acid extraction reagent system, whose sample preservation solution contains 5% dithiothreitol and 2.5% potassium azide for simultaneous sample liquefaction and salt ion chelation during transportation; and an amplification and library construction reagent system, which contains a primer mixture consisting of 175 primer pairs, E. coli synthase III, and separately added magnesium ions. By integrating the above reagent systems and optimizing the entire process, this invention reduces the total time from sample pretreatment to report generation to less than 2.5 hours. This invention has the advantages of extremely fast detection speed, wide sample applicability, high operational stability, and ease of automation.

The application discloses a method and a kit for immunodetection of target substances by using arterial blood, and belongs to the technical field of blood detection. The kit comprises at least: a first component containing luminescent microspheres combined with detection antibody 1 and a luminescent microsphere diluent, wherein the luminescent microsphere diluent contains 2-morpholinoethanesulfonic acid, KCl and NaHCO3; a second component containing biotin-labeled detection antibody 2 and a biotin diluent, wherein the biotin diluent contains dithiothreitol, CaCl2, adenine nucleoside and mouse anti-human hemoglobin antibody; and a third component containing photosensitive microspheres combined with streptomycin. The scheme can be used for immunodetection of target substances by using arterial blood, has excellent functional sensitivity and detection range, is not interfered by other substances in arterial blood, realizes rapid detection of all blood gas indexes and immunological indexes by using one tube of arterial blood, and has important significance for clinical blood detection.

The invention discloses a multi-target synergistic anti-wrinkle firming beauty makeup composition as well as a preparation method and application of the multi-target synergistic anti-wrinkle firming beauty makeup composition. The technical defects that an existing polypeptide product is single in effect, low in permeability and poor in stability are effectively overcome. The composition takes acetyl hexapeptide-8, palmitoyl tripeptide-1 and decapeptide-4 as core active components, is compounded with auxiliary materials such as a humectant, a penetration enhancer, a stabilizer and a preservative, and is prepared by compounding according to a specific mass percent. The action mechanisms of'inhibiting dynamic wrinkles, promoting collagen synthesis and protecting a collagen structure 'of the three polypeptides are complementary, so that multi-target synergistic anti-wrinkle is realized; the skin permeability is improved by means of plant-derived ceramide NP, and the polypeptide stability is enhanced through dithiothreitol and pH regulation. The invention can be widely applied to beauty makeup products such as essence, face cream, eye cream and the like.

The invention discloses grape pomace pretreatment liquid and a method for preparing microcrystalline cellulose from grape pomace. The grape pomace pretreatment liquid is prepared from laccase, polygalacturonase, acetosyringone, sodium dihydrogen phosphate, disodium hydrogen phosphate, dithiothreitol, fatty alcohol-polyoxyethylene ether, sorbitol and the balance of water. The method for preparing the microcrystalline cellulose from the grape pomace comprises the following steps: S1, cleaning the grape pomace with normal hexane, then cleaning with water, drying and crushing; s2, carrying out mixed reaction with the pretreatment liquid, and then separating out pretreatment residues; s3, adding a sodium carbonate solution for premixing, then adding a tetramethylammonium hydroxide solution and a sodium borohydride solution, and filtering after reaction to obtain filter residues; s4, adding a citric acid solution, adding carboxymethyl chitosan, adsorbing, and filtering to obtain cellulose residues; and S5, hydrolyzing and depolymerizing by using dilute acid, neutralizing, separating and washing an acidolysis product, and drying to obtain the microcrystalline cellulose. According to the method, impurity components which are difficult to treat in the grape pomace can be overcome, impurity removal and purification are completed, cellulose is reserved to the maximum extent, loss is reduced, and the yield is remarkably increased while high-purity microcrystalline cellulose is obtained.

Presented herein are methods for making, isolating, and purifying N-acetylcysteine amide, (2R,2R′)-3,3′-disulfanediyl bis(2-acetamidopropanamide, diNACA), intermediates and derivatives thereof comprising: alternatively contacting cystine with methanol and a chlorinating reagent to form an organic solution containing L-cystine dimethylester dihydrochloride; combining dried or undried L-cystine dimethylester dihydrochloride with a triethylamine, an acetic anhydride, and an acetonitrile to form a di-N-acetylcystine dimethylester; mixing dried di-N-acetylcystine dimethylester with ammonium hydroxide to form a di-N-acetylcystine amide (diNACA); and separating dried di-N-acetylcystine dimethylester into N-acetylcysteine amide with dithiothreitol, triethylamine, and an alcohol.

The invention relates to a hair lysate and application thereof. The hair lysate is prepared from the following components in percentage by volume: 0.05 to 1.0 percent of ethyl phenyl polyethylene glycol, 2 to 18 mg / mL of dithiothreitol and 2 to 20 U / mL of protease. The NP-40 is a surfactant, the dithiothreitol is a reducing agent, and the NP-40 and the dithiothreitol jointly act with the protease, so that drug components possibly existing in the hair can be quickly released, ultrasonic or freezing grinding is not needed, and the operation is simple and convenient. The hair lysate can meet the requirement of qualitative detection only after being treated for 10 minutes, the best effect can be obtained if the treatment time is as long as 30 minutes, the release efficiency is superior to that of a traditional method, and whether a hair sample contains trace drugs or not can be accurately detected by matching with subsequent liquid chromatography-tandem mass spectrometry analysis.

This invention belongs to the field of surgical wound repair technology, and provides a multifunctional composite hydrogel, its preparation method, and its applications. The invention involves mixing fibrinogen, mussel byssal protein, calcium chloride, methacrylated dextran (Dextran-MA), thrombin, dithiothreitol, and a dispersing solvent. The resulting mixture is then gelled to obtain a multifunctional composite hydrogel. Thrombin specifically promotes the cross-linking of fibrinogen to form a biomolecular network; dithiothreitol efficiently mediates the formation of an organic macromolecular network through a Michael addition reaction of Dextran-MA; the introduction of Mfp5, utilizing its abundant catechol groups to form multiple coordination and covalent cross-links with organic / biomolecular macromolecules, significantly enhances the interfacial adhesion strength of the multifunctional composite hydrogel; simultaneously maintaining the biocompatibility, biodegradability, hemostatic properties, anti-inflammatory effects, and healing-promoting capabilities of the multifunctional composite hydrogel.

The invention relates to hydrogel microparticles, compositions and uses thereof. The present invention provides spherical or elliptical non-alginate hydrogel microparticles for local delivery and sustained release of therapeutic cells and / or tissues at an implantation site, the microparticles comprising a 3-dimensional substrate of a covalently crosslinked non-alginate polymer compound and a therapeutically effective amount of cells and / or tissues embedded therein, wherein the cells have a viability of at least 50%, the microparticles have a size of greater than 30 [mu] m, the non-alginate hydrogel microparticles are formed without using an oil emulsion, the polymer compound is polyethylene glycol vinyl sulfone or acrylated hyaluronic acid, the matrix further includes a cross-linking agent selected from the group consisting of dithiothreitol, polyethylene glycol dithiol, and ethylene glycol bis-mercaptoacetate, cross-linked with the non-alginate polymer compound. Compositions comprising such hydrogel microparticles and their use for the preparation of medicaments are also described.

The present invention belongs to the technical field of preparing functional materials for ion recognition, adsorption, and separation, and discloses a method for preparing a functionalized light-driven nanomotor adsorbent and its adsorption application. The present invention uses a solvent method to load gold nanoparticles (Au NPs) inside HNTs to produce a good photothermal effect. The surface is then covered with a layer of polydopamine (PDA) and dithiothreitol succinate (DMSA), thereby improving the adsorption performance of the adsorbent. Experiments have shown that Au@DHNTs-DMSA has good photothermal conversion efficiency, biocompatibility, and biosafety, and an in vitro blood lead removal rate of up to 91.25%, making it effective in treating lead poisoning.

The application belongs to the field of modified organosilicon preparation, and discloses a preparation method and application of an in-situ hydroxylated organosilicon composite film, which comprises the following steps: (1) dissolving ethylene bridge organosilicon precursor (BTESEthy), dithiothreitol (DTT) and a photoinitiator in a small amount of anhydrous ethanol under a nitrogen atmosphere, and irradiating the mixture with ultraviolet light in a light-shielded environment to initiate a click reaction, thereby obtaining in-situ hydroxylated organosilicon precursor; (2) coating the obtained in-situ hydroxylated organosilicon sol on a PVDF film containing GO nanosheet layers on the surface, and finally obtaining the in-situ hydroxylated organosilicon composite film. The in-situ hydroxylated organosilicon composite film provided by the application is applied to the field of membrane separation, and effectively improves the separation and permeation performance of organic matter and water.

The invention discloses a method for detecting a membranous nephropathy target antigen, which comprises the following steps: adding a protein extracting solution into a glomerular tissue to be detected, and incubating; adding a mixed enzyme of a LysC enzyme and a Trypsin enzyme, and incubating; adding a dithiothreitol solution, and incubating; adding an iodo-acetamide solution, and incubating at room temperature in a dark place; adding a precipitating agent, performing ultrasonic treatment and centrifugation, taking supernate, loading the supernate to a C18 small column, eluting the small column, and collecting eluent; freezing the eluent, blow-drying the eluent, and redissolving the eluent with a loading solution to obtain a test solution; and carrying out liquid chromatography-tandem mass spectrometry detection on the test solution. By adopting the detection method disclosed by the invention, the target antigen of membranous nephropathy can be accurately detected when the sampling volume is obviously reduced, so that the sampling difficulty is greatly reduced, and the application of the laser microdissection and liquid chromatography-mass spectrometry combined technology in the detection of the target antigen of membranous nephropathy is greatly promoted. The method is simple in process and short in detection time.

A composition for improving the detection performance of fluorescent quantitative PCR. The composition comprises bovine serum albumin, sorbitol, ammonium sulfate, formamide, tetramethylammonium chloride, and at least one of dithiothreitol and betaine. The present invention further relates to a qPCR reaction liquid containing the composition and a preparation method therefor. The composition can improve the sensitivity, specificity, and interference resistance of real-time fluorescent quantitative PCR.

The invention belongs to the technical field of agricultural biology, and discloses a non-tissue culture dependent transformation method for promoting effective transformation of potatoes by using growth factors. The invention discloses a reagent composition. The reagent composition comprises an infection solution and a bud inducing screening solution, wherein the infection liquid is prepared from acetosyringone, dithiothreitol and plant hormones; the bud inducing screening liquid comprises plant hormones, glufosinate-methyl and regeneration factors. The invention provides a reagent composition. The reagent composition can be used for non-tissue culture dependent genetic transformation of potatoes. By using the reagent composition, the non-tissue culture dependent genetic transformation step of the potatoes can be simplified, the conditions of chimera and false positive of transformants are reduced, and the transformation efficiency is remarkably improved.

The invention discloses application of a low-preference polymerase chain reaction buffer solution and a product, and relates to the technical field of molecular biology. The low-preference polymerase chain reaction buffer solution is prepared from 20 to 35 mM of Tris-HCl, 15 to 30 mM of K < + >, 1.5 to 2.2 mM of Mg < 2 + >, 0.6 to 1.0 M of betaine, 2.5 to 4.0 percent of dimethyl sulfoxide, 0.3 to 0.8 mM of ferrous sulfate heptahydrate, 0.2 to 0.6 mM of trehalose, 0.5 to 1.5 percent of NP-40, 0.2 to 0.5 mg / mL of recombinant escherichia coli single-chain binding protein and 0.5 to 1.0 mM of dithiothreitol; on the premise that the amplification yield is not reduced, the human whole genome GC preference coefficient is reduced to be smaller than or equal to 0.1, the coverage uniformity CV is smaller than or equal to 0.18, 0.1-50 ng of initial DNA is compatible, and the chromosome abnormality detection accuracy is improved.

This invention discloses a neutral dissociation agent and its preparation method for detecting the content of 25-hydroxyvitamin D in human blood samples, belonging to the field of biomedical detection technology. The dissociation agent is composed of disodium EDTA, sodium dodecyl sulfonate, dithiothreitol, hexadecyltrimethylammonium bromide, and hydroxypropyl-β-cyclodextrin dissolved in 50 mM tris(hydroxymethyl)aminomethane buffer, with the mass-volume ratios of each component being 0.5%-1%, 0.5%-1%, 0.2%-0.5%, 0.2%-0.5%, and 0.2%-0.5%, respectively. During preparation, each component is dissolved according to the steps, and the pH is adjusted to 7.4. This dissociation agent exhibits mild dissociation conditions, does not damage protein activity, has high dissociation efficiency, and is non-corrosive and non-toxic. The detection results show high specificity and sensitivity, with a coefficient of variation ≤6%. It can be directly integrated into automated detection processes and is suitable for large-scale clinical and research applications.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to an indometacin liniment for external use and a preparation process thereof, the indometacin liniment is prepared by compounding indometacin, a composite solubilizer, a composite stabilizer, a penetration enhancer, a humectant, a pH regulator and a solvent; wherein the composite solubilizer is a compound system of ethanol, N-sodium methyl taurate, lauroyl lysine and propylene glycol, the composite stabilizer is a compound system of ethylene diamine tetraacetic acid, acetylcysteine and dithiothreitol, the penetration enhancer is peppermint oil, the humectant is glycerol, the pH regulator is triethanolamine / citric acid, and the solvent is purified water. Through the specific compounding design of the components and the precise control of the preparation process, the obvious improvement of the water solubility of the indometacin and the effective guarantee of the content stability of the preparation are synchronously realized.

The application discloses a clinical biochemical composite quality control product, which comprises detection item raw materials, a matrix liquid and a protective agent; the detection item raw materials are alkaline phosphatase, amylase, sodium glycocholate, cholinesterase, creatinine, leucine aminopeptidase, lactate dehydrogenase, lipase, triglyceride, cholesterol, uric acid, urea, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, glucose, alpha-hydroxybutyric acid dehydrogenase, sodium hydrogen phosphate, magnesium chloride, calcium chloride, ferrous chloride, bilirubin, pancreatic amylase and sodium bicarbonate; the matrix liquid is bovine serum albumin and human serum albumin; and the protective agent is dithiothreitol (DTT), mannitol, trehalose, sodium chloride, a composite enzyme stabilizer AES and sodium azide. The quality control product can realize the detection of 27 clinical biochemical items, and the freeze-drying process is adopted, so that the freeze-dried composite quality control product has low viscosity, is easy to be re-dissolved and mixed, has high stability and has small matrix effect.

The invention discloses a PCR (Polymerase Chain Reaction) buffer solution, a kit, a fully premixed storage solution and application. The PCR buffer solution comprises the following components: a buffer agent, potassium salt, magnesium salt, ammonium sulfate, dNTP (T), dNTP (U), Triton X-100, dimethyl sulfoxide, tetramethylammonium chloride, glycerol, dithiothreitol and bovine serum albumin. According to the PCR buffer solution provided by the invention, the amplification efficiency of a high-GC-content template is remarkably improved through a synergistic effect generated by all the components in a specific ratio, the premixing performance is effectively enhanced, and the reagent stability after the PCR buffer solution is premixed with enzyme, primers and probes is remarkably improved.

The present application relates to a kind of low sensitization casein preparation method and low sensitization casein obtained using the preparation method, belong to food biotechnology field;The inventor of the present application accidentally found that when using the combination of "sodium dodecyl sulfate and dithiothreitol" specific proportion is handled to casein, the sensitization of linear casein after development is significantly reduced;Specifically, after treatment, the globular protein structure of casein is not only developed into linear protein structure, but also unexpectedly found that aggregation exists between protein molecules, and the effects of development and aggregation together lead to most of the sensitization sites on casein being covered, thereby reducing the sensitization of casein;The method of the present application is simple and low in cost, can significantly reduce the sensitization of casein without destroying the nutritional quality of casein itself.

The invention discloses a multi-volume and multi-form STR (short tandem repeat) multiplex fluorescence multiplex amplification detection freeze-drying reagent as well as a preparation method and application thereof. The freeze-drying reagent consists of an STR multiplex fluorescence multiplex amplification system and a freeze-drying protective agent system, the STR multiplex fluorescent multiplex amplification system is composed of a 5X amplification buffer, a 5X primer mixture and Taq DNA polymerase; the 5X amplification buffer is prepared from Tris-HCl, ammonium sulfate, 20% Tween, magnesium chloride, bovine serum albumin, potassium chloride, ethylenediamine tetraacetic acid and dithiothreitol; the 5X primer mixture is a mixture of primers in a DNATperTM 30 kit of the Ministry of Public Security Identification Center; the freeze-drying protective agent system is composed of cane sugar, trehalose, polyethylene glycol 8000 and D-mannitol. The raw materials are freeze-dried in an in-situ freeze-drying or microsphere freeze-drying mode. According to the invention, the technical problems that the stability of the STR multiple fluorescent multiplex amplification detection freeze-drying reagent is poor and the detection accuracy is influenced by a freeze-drying protective agent due to the incoordination of an STR multiple fluorescent multiplex amplification system and a freeze-drying protection system can be solved.