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A technology for detecting kits and albumin, applied in biological testing, measuring devices, material inspection products, etc., to achieve the effects of ensuring high-efficiency detection, promoting binding speed, and protecting stability
Active Publication Date: 2014-04-30
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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[0014] In order to solve the problems of low detection accuracy, poor stability and low detection efficiency of IMA existing in the prior art, the present invention provides a high detection accuracy, stable Ischemia-modified albumin detection kit with good performance and high detection efficiency
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Embodiment 1
[0048] The components and concentrations of reagent 1 are:
[0049]Tris buffer (pH 7.6) 0.1mol / L,
[0050] Cobalt chloride 20mmol / L,
[0051] PC-300 1g / L,
[0052] The components and concentrations of reagent 2 are:
[0053] Imidazole buffer (pH 7.0) 1mol / L,
[0054] Dithiothreitol 20mmol / L,
[0055] PC-300 1g / L.
[0056] The formula in Example 1 is a formula approved by the FDA for routine use in the market, and is used as a theoretical comparison.
[0057] The preparation method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and mixed well.
[0058] The test conditions for the IMA in the test kit of the present invention to measure the sample are as follows:
[0059] Temperature: 37 °C; the light path of the cuvette is 1.0 cm. Detection wavelength 510 nm. (Now clinical automatic biochemical analysis instruments can meet this requirement)
[0060] The method for applying the IMA as...
Embodiment 2
[0066] The components and concentrations of reagent 1 are:
[0067] Tris buffer (pH 7.6) 0.1mol / L
[0068] Cobalt chloride 20mmol / L
[0069] PC-300 1g / L
[0070] The components and concentrations of reagent 2 are:
[0071] Imidazole buffer (pH 7.0) 1mol / L
[0072] Dithiothreitol 20mmol / L
[0073] Mercaptoethanol 6mol / L
[0074] PC-300 1g / L
Embodiment 3
[0077] The components and concentrations of reagent 1 are:
[0078] Tris buffer (pH 7.6) 0.1mol / L
[0079] Cobalt chloride 20mmol / L
[0080] PC-300 1g / L
[0081] The components and concentrations of reagent 2 are:
[0082] Imidazole buffer (pH 7.0) 1mol / L
[0083] Dithiothreitol 20mmol / L
[0084] Sodium bisulfite 6mol / L
[0085] PC-300 1g / L
[0086] The preparation method of the kit in Example 3 is the same as in Example 1, and the detection method is the same as in Example 1.
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Abstract
The invention relates to the technical field of detection of ischemia modified albumin content, and in particular to a detection kit for ischemia modified albumin. The kit includes a reagent 1 and a reagent 2. The reagent 1 contains a buffer, cobalt chloride, a stabilizer and a preservative; and the reagent 2 contains a buffer, dithiothreitol, a stabilizer, a reducing protective agent and a preservative. The kit can accelerate the binding efficiency of cobalt ions with normal albumin, and the cobalt ions fully integrate with normal albumin in a certain period of time, so as to ensure the accuracy of the detection result on a sample by the reagent; the kit protects the stability of dithiothreitol in the solution, and does not affect the binding of dithiothreitol with cobalt ions, so as to ensure that an open reagent bottle can be stably kept in a dark place for 30 days at room temperature and a closed reagent bottle can be stably kept at 2-8 DEG C for 12 months, and fully meet the needs of clinical laboratory; and the kit accelerates the combination of dithiothreitol with cobalt ions, so that the reagents reach reaction endpoints as soon as possible, thereby ensuring the efficient detection effect of reagents and significantly improving the accuracy of reagent detection.
Description
technical field [0001] The invention relates to the technical field of detection of ischemic modified albumin (IMA), in particular to a detection kit for ischemic modified albumin. Background technique [0002] IMA is a sensitive indicator of early myocardial ischemia, combined with other blood biochemical indicators can improve sensitivity and specificity, mainly used for early diagnosis of ACS patients, also used for PCI, pacemaker and defibrillator implantation, expansion Evaluation of the prognosis of patients with acute cardiomyopathy and acute myocardial ischemia. [0003] Complete albumin is synthesized in the liver, consisting of 585 amino acid residues, with a relative molecular weight of 66.5 KD, a blood concentration of 0.63 mmol / L, and a half-life of 19-20 days. Its amino terminal is a sequence unique to human beings, which can combine with transition metals such as cobalt, copper, nickel, etc. under normal physiological conditions. [0004] During ischemia a...
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