38 results about "Ischemia-modified albumin" patented technology

The invention relates to the technical field of detection of ischemia modified albumin content, and in particular to a detection kit for ischemia modified albumin. The kit includes a reagent 1 and a reagent 2. The reagent 1 contains a buffer, cobalt chloride, a stabilizer and a preservative; and the reagent 2 contains a buffer, dithiothreitol, a stabilizer, a reducing protective agent and a preservative. The kit can accelerate the binding efficiency of cobalt ions with normal albumin, and the cobalt ions fully integrate with normal albumin in a certain period of time, so as to ensure the accuracy of the detection result on a sample by the reagent; the kit protects the stability of dithiothreitol in the solution, and does not affect the binding of dithiothreitol with cobalt ions, so as to ensure that an open reagent bottle can be stably kept in a dark place for 30 days at room temperature and a closed reagent bottle can be stably kept at 2-8 DEG C for 12 months, and fully meet the needs of clinical laboratory; and the kit accelerates the combination of dithiothreitol with cobalt ions, so that the reagents reach reaction endpoints as soon as possible, thereby ensuring the efficient detection effect of reagents and significantly improving the accuracy of reagent detection.

The invention discloses a preparation method of quality control serum for ischemia-modified albumin; the preparation method comprises the following steps of: (1) adopting the blood serum of a healthy person, and adjusting a pH value to be 7.0-7.5; (2) adding 0.1-100mm of metal ionic salt with a variable valence center or a metal organic composite with molecule planarity to the blood serum, and then adding 0.1-100mm of hydrogen peroxide; (3) reacting for 10-30min under the 35-40 DEG C illumination; (4) dialyzing a solution obtained through the reaction in the step (3) in 0.1-1000mm of a dialysis buffer solution with pH of 7.5-8.0 under the 20-25 DEG C temperature for 12-24h; and (5) adding a preservative according to a conventional technology, subpackaging and determining after mixing uniformly. The preparation method has the advantages of adequate the source of raw materials, easily obtaining of the raw materials, simple preparation technology, few amount of additives, low production cost, small non-specificity interference, no needing for composite dissolving, good homogeneity and small difference between bottles.

The invention discloses a chip for rapidly detecting ischemia modified albumin in blood. The chip comprises a non-absorbent bottom plate and a non-absorbent cover plate, wherein a sample tank, a mixing tank and a detection tank are arranged on the bottom plate in sequence; micro-pipelines are arranged between the sample tank and the mixing tank and between the mixing tank and the detection tank; the sample tank is internally filled with a sample pad; the sample pad is coated with a cobalt standard product; the detection tank is internally filled with a detection pad; the detection pad is coated with a cobalt indicator; the cover plate is fixedly arranged above the bottom plate; and the cover plate is located above the sample tank and is provided with a sample feeding hole. The chip disclosed by the invention is simple in detection method, and a result is easy to judge; and the chip is suitable for being used in medical units including primary-level hospitals, emergency departments and the like or on bedsides of hospitalized patients, so that medical workers can rapidly judge dangers of the patients by layers and medical resources and health cost are saved.

A method for assessing liver function in an individual, which method comprises determining the level of methylarginine(s) (such as ADMA and / or SDMA) and the ratio of ischemia modified albumin (IMA): albumin ratio (IMAR) in the individual, thereby to assess liver function in the individual.

The invention discloses a stable ischemia modified albumin testing kit which comprises a cobalt ion reagent and a dithiothreitol (DTT) reagent. A small amount of nonionic surfactant is added in the dithiothreitol reagent, thus the combination of DTT in the reagent and free oxygen is greatly reduced, the oxidation speed of DTT is reduced, and the stability of the reagent is remarkably improved. Simultaneously, the concentration of DTT is increased, thus the stability of the kit is improved to a certain extent. The stable ischemia modified albumin testing kit disclosed by the invention can be stored stably in an opened bottle in a dark place at the room temperature for 30 days, and also can be stored stably in a closed bottle at the temperature of 2 to 8 DEG C for 12 months, and can totally meet the requirements for clinical examinations.

The invention discloses an ischemia modified albumin liquid stabilization kit. The kit is a liquid dual reagent of a reagent 1 and a reagent 2, wherein the reagent 1 comprises a buffer solution of which the pH is 7.5 to 8.5 and the concentration is 0.05 to 0.5M, cobalt chloride at the concentration of 20 to 200mg / L, an interference removing agent at the concentration of 0.1 to 10g / L, a stabilizing agent at the concentration of 0.1 to 10g / L, and preservative at the concentration of 0.1 to 10g / L; and the reagent 2 comprises a buffer solution of which the pH is 7.0-8.0 and the concentration is 0.5 to 10M, dithiothreitol at the concentration of 0.5 to 50g / L, a stabilizing agent at the concentration of 0.1 to 10g / L, and preservative at the concentration of 0.1 to 10g / L. The kit has the advantages of fully meeting the clinical inspection requirement, along with high accuracy and stability, and can be stabilized for 12 months when stored at the temperature of between 2 and 8 DEG C in a dark place.

The invention relates to the field of biotechnologies, and discloses a detection kit for ischemia modified albumin and a preparation method of the detection kit. The kit comprises a reagent R1and a reagent R2, wherein stabilizing agents of the R2 comprise ethanolamine, a nonionic surfactant and 2,2'-diethylamino dithio dihydrochloride, and the massic volume of ethanolamine is 20%-80%. According to the preparation method, by adding ethanolamine, the nonionic surfactant and 2,2'-diethylamino dithio dihydrochloride into the reagent R2, dithiothreitol (DTT) in the reagent R2 of the kit can not be easily oxidized and is long in half-life period, therefore, the detection kit for the ischemia modified albumin is high in stability, good in sample correlation and high in anti-jamming capability.

The invention provides a method for detecting ischemia modified albumin. According to the method, the characteristic that the binding capacity of the ischemic modified albumin and the cobalt is weakened is utilized, free cobalt in a reaction system is detected by using chemical reagents, the change of the content of the free cobalt is expressed through the change of absorbance, the concentration of the ischemia modified albumin is indirectly expressed, and a color developing agent used in the detection is 2,4-dimercaptopyrimidine and is used for detecting the free cobalt in the reaction system. The method has the advantages that the stability of the color developing agent of the 2,4-dimercaptopyrimidine is higher, the analysis sensitivity is higher, the price is lower, the detection resultis reported based on the concentration of the free cobalt, and the problem that the absorbance of an IMA (ischemia modified albumin) detection result is unstable due to the fact that the performanceof instruments or reagents is different and unstable when the absorbance is adopted for report can be solved. The invention further provides a detection kit for the ischemia modified albumin. The detection kit comprises the reagents used in the method.