9 results about "Fatty acid binding" patented technology

The invention discloses a method for simply and efficiently promoting mycelial growth and non-co-spore production of AM (arbuscular mycorrhizal) fungi and application of the method. According to the method, a dual-cultured AM fungus fungicide is refrigerated and preserved for a long time, and the preservation time is 3-38 months. The spores obtained after long-term preservation are inoculated on a culture medium under a non-symbiotic condition, and C14 fatty acid is added into the culture medium. After C14 fatty acid is exogenously added into the spores refrigerated for a long time, the amount of hyphae and the production amount of the spores formed by AM fungus non-symbiosis can be remarkably increased, and the density of the hyphae is 7.3 times that of the hyphae without the fatty acid; each mother spore can form about 54 spores, and the yield of the spores is about 10 times that of the non-symbiotic spores treated by adding C14 fatty acid in combination with an exogenous stimulant. According to the method, the AM fungus fungicide can be propagated under the non-symbiotic condition, the propagation efficiency is high, the non-symbiotic sporulation quantity of AM fungi can be remarkably increased, operation is easy and convenient, the cost is low, the period is short, and wide application and popularization prospects are achieved.

Cosmetic coated particles having, on at least a portion of the surface of particles (B), a coating layer comprising an ester compound (A) and a powder of leaves of at least one plant selected from the genera Camellia, Olive, Diospyros, Curcuma, Quercus, Luffa, Perilla, Ginkgo, Equisetum, Artemisia, Cycad, Mulberry, and Eucommia, wherein the ester compound (A) is an ester compound of a C8-28 fatty acid and a sugar compound formed by combining two or more monosaccharides.

This invention discloses a method for determining the distribution of fatty acids at the sn-2 position in oils rich in long-chain polyunsaturated fatty acids, belonging to the fields of analytical chemistry and food science. The method introduces a quality control oil sample containing a correction fatty acid, which is bound to any acyl position in the triglyceride molecule. A correction factor is calculated from the measured data of the quality control oil sample, and this correction factor is used to correct for enzymatic hydrolysis efficiency and analytical system bias. This invention innovatively introduces a quality control sample to monitor and correct enzymatic hydrolysis efficiency and separation recovery rate, eliminating systematic errors caused by factors such as enzyme activity fluctuations and operational differences, and greatly improving the reproducibility of the method across different times and operators.

The present application relates to a kind of synthetic polyunsaturated fatty acid binding phospholipid yeast engineering bacteria and construction method and application, belong to microbial engineering technical field, the engineering bacteria is with Yarrowia lipolytica as bottom cell, knock out the Mhy1, Iro1, IDH1, CEX1 gene in its genome for generating mycelium, synthesis of triglyceride, citric acid metabolism, citric acid efflux;Phospholipid synthesis, pyruvate synthesis, polyunsaturated fatty acid synthesis and acyltransferase CDS1, OPI3, Mce2, LplA, △9Elo, △8Des, △5Des, △17Des, △5Elo, △15Des, LPCAT3 gene are sequentially connected together, as an expression module is integrated in the URA site of genome.The yeast engineering bacteria of the present application obtains polyunsaturated fatty acid binding phospholipid in one step efficiently, and phospholipid yield is 151.19 mg / g, and in binding fatty acid, EPA content is 13.2%.

The present invention relates to MC4R (Melanocortin-4 Receptor) agonist compounds with fatty acid binding technology optimized for improved in vivo stability, and pharmaceutical compositions, cosmetic compositions, and health functional foods containing the same.

The invention discloses a preparation method of Korean pine kernel oil sterol with a blood fat reducing function. The preparation method sequentially comprises the steps of enzymolysis, fatty acid combination and separation, light-wave-assisted extraction and purification. The method comprises the following steps: firstly, carrying out enzymolysis on triglyceride in the red pine nut oil by adopting composite lipase to hydrolyze the triglyceride into fatty acid and glycerol; then adding a mixed alkali solution to be combined with fatty acid to form water-soluble salt, and creating conditions for sterol extraction; a light wave-assisted extraction technology is adopted, an n-hexane and limonene composite solvent is adopted for extraction, a polyethylene glycol aqueous solution is adopted for washing, and the solubility and the extraction rate of sterol are effectively improved; and finally purifying through macroporous resin combined magnetic field assisted solvent crystallization. The method maximally retains the natural activity of sterol, has the advantages of high extraction rate, mild conditions and good product purity, and ensures the functional activity of lowering blood fat.

Disclosed herein is a meat analogue composition comprising from 2% to 81% by weight of a fat composition; from 1% to 30% by weight of a non-animal protein; and from 18% to 70% by weight of water; wherein the fat composition comprises from 10% to 50% by weight of saturated fatty acid residues; from 5% to 50% by weight of stearic acid residues (C18:0); from 5 to 35% by weight of linoleic acid residues (C18:2); and from 1 to 15% by weight of a-linolenic acid residues (C18:3); wherein said percentages of fatty acid residues refers to fatty acids bound as acyl groups in glycerides in the fat composition and being based on the total weight of C6 to C24 fatty acid residues bound as acyl groups present in the fat composition.

An oil composition comprises from 1% to 20% by weight of caproic acid residues (C6:0); at least 55% by weight of C16 to C18 unsaturated fatty acid resides; and less than 40% by weight of C8 to C24 saturated fatty acid residues; wherein said percentages of fatty acid residues refers to fatty acids bound as acyl groups in glycerides in the oil composition and being based on the total weight of saturated and unsaturated C4 to C24 fatty acid residues bound as acyl groups present in the oil composition; and wherein at least 50% of the caproic acid residues present in the oil composition are present in a triglyceride comprising at least one caproic acid residue and at least one fatty acid residue comprising from 16 to 18 carbon atoms.

The application belongs to the field of light industry grease, and discloses a method for detecting and analyzing the content of positional isomer of same-acid glyceride by high performance liquid chromatography tandem mass spectrometry. Based on the derivatization treatment of same-acid glyceride, the hydroxyl group in the glyceride not combined with the fatty acid is connected with a new group (isocyanate), and the corresponding 1,2-DAG isocyanate derivative or 1,3-DAG isocyanate derivative is generated. The same-acid glyceride derivative is analyzed by using a liquid chromatograph-mass spectrometer, and the content of the positional isomer of same-acid glyceride is quantitatively analyzed by the relative content difference of two daughter ions generated under collision-induced dissociation of the derivative. The method can realize the quantification of the positional isomer of glyceride without separating the positional isomer of glyceride by chromatography, and only the conventional C18 chromatography is used, so that the development of special chiral chromatographic column or specific chromatographic separation technology is reduced.