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Kit for detecting content of heart-type fatty acid binding protein by latex enhanced immunoturbidimetric assay

A fatty acid binding and immunoturbidimetric technology, applied in the biological field, can solve the problems of self-coagulation, poor storage stability of reagents, and low detection accuracy.

Pending Publication Date: 2019-12-20
SHANGHAI JIEMEN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing latex-enhanced immunoturbidimetric method also has huge technical difficulties, for example, low detection sensitivity; poor storage stability of reagents, self-coagulation phenomenon; low detection accuracy and other shortcomings

Method used

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  • Kit for detecting content of heart-type fatty acid binding protein by latex enhanced immunoturbidimetric assay
  • Kit for detecting content of heart-type fatty acid binding protein by latex enhanced immunoturbidimetric assay
  • Kit for detecting content of heart-type fatty acid binding protein by latex enhanced immunoturbidimetric assay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] The kit of the present invention is liquid dual reagents, which are respectively reagent R1 and reagent R2, wherein the ratio of reagent R1 to reagent R2 is 1:1. in,

[0105] The formula of reagent R1 is as follows:

[0106]

[0107] Reagent R2 contains the following components:

[0108]

[0109] Preparation of reagents:

[0110] 1. The preparation of R1: Dissolve each component in purified water according to the content, mix well, and constant volume.

[0111] 2. Preparation of the heart-type fatty acid binding protein monoclonal antibody labeled with horseradish peroxidase:

[0112] (1) Dissolve 5mg of horseradish peroxidase (HRP) in 0.5ml of distilled water, add freshly prepared 0.06mol / L sodium periodate (NaIO 4 ) solution 0.5ml, mix well and place in the refrigerator to avoid light for 30min.

[0113] (2) Take it out, add 0.5ml of 0.16mol / L ethylene glycol aqueous solution, and let it stand at room temperature for 30min.

[0114] (3) Add 1ml of 5mg / mL h...

Embodiment 2

[0130] The kit of the present invention is liquid dual reagents, which are respectively reagent R1 and reagent R2, wherein the ratio of reagent R1 to reagent R2 is 1:1. in,

[0131] The formula of reagent R1 is as follows:

[0132]

[0133] Reagent R2 contains the following components:

[0134]

[0135]

[0136] Preparation of reagents:

[0137] 1. The preparation of R1: Dissolve each component in purified water according to the content, mix well, and constant volume.

[0138] 2. Preparation of the heart-type fatty acid binding protein monoclonal antibody labeled with horseradish peroxidase:

[0139] (1) Dissolve 5mg of horseradish peroxidase (HRP) in 0.5ml of distilled water, add freshly prepared 0.06mol / L sodium periodate (NaIO 4 ) solution 0.5ml, mix well and place in the refrigerator to avoid light for 30min.

[0140] (2) Take it out, add 0.5ml of 0.16mol / L ethylene glycol aqueous solution, and let it stand at room temperature for 30min.

[0141] (3) Add 1ml...

Embodiment 3

[0152] The kit of the present invention is liquid dual reagents, which are respectively reagent R1 and reagent R2, wherein the ratio of reagent R1 to reagent R2 is 1:1. in,

[0153] The formula of reagent R1 is as follows:

[0154]

[0155] Reagent R2 contains the following components:

[0156]

[0157] Preparation of reagents:

[0158] 1. The preparation of R1: Dissolve each component in purified water according to the content, mix well, and constant volume.

[0159] 2. Preparation of the heart-type fatty acid binding protein monoclonal antibody labeled with horseradish peroxidase:

[0160] (1) Dissolve 5mg of horseradish peroxidase (HRP) in 0.5ml of distilled water, add freshly prepared 0.06mol / L sodium periodate (NaIO 4 ) solution 0.5ml, mix well and place in the refrigerator to avoid light for 30min.

[0161] (2) Take it out, add 0.5ml of 0.16mol / L ethylene glycol aqueous solution, and let it stand at room temperature for 30min.

[0162] (3) Add 1ml of 5mg / mL h...

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Abstract

The invention relates to a kit for detecting content of heart-type fatty acid binding proteins by latex enhanced immunoturbidimetric assay. Specifically, the kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains a buffer solution, an electrolyte, a turbidity increasing agent and a preservative; and the reagent R2 contains horse radish peroxidase labeled heart-type fatty acidbinding protein monoclonal antibody latex particles, a stabilizer, a buffer solution, an electrolyte, a protective agent and a preservative. The kit disclosed by the invention has the advantages of high sensitivity, good stability and high precision, and can be applied to various biochemical analysis instruments.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for detecting the content of heart-type fatty acid-binding protein by latex-enhanced immune turbidimetry, and more particularly to a method for detecting human serum heart-type fatty acid-binding protein by using latex-enhanced immune turbidimetry The kit of content and its preparation method and application. Background technique [0002] Heart-type fatty acid binding protein (h-FABP) is a new type of acidic small cytoplasmic protein with an isoelectric point (PI) of 5.1, which is abundant in the heart. It consists of 132 amino acids with a molecular weight of 15kDa. It is highly cardiac-specific, mainly present in cardiomyocytes, but also expressed at low concentrations in tissues other than the heart, such as trace amounts in skeletal muscle. h-FABP can combine with long-chain fatty acids in the myocardium, transport them to the mitochondria, and finally oxidize and decompo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/535G01N33/543G01N33/577G01N33/58
CPCG01N33/6887G01N33/535G01N33/577G01N33/581G01N33/54313
Inventor 王丽娇
Owner SHANGHAI JIEMEN BIO TECH
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