29 results about "Protein.monoclonal" patented technology

The application belongs to the technical field of biology and particularly relates to an African swine fever virus pNP868R protein monoclonal antibody and a preparation method and application thereof. The application takes the pNP868R protein prepared by eukaryotic expression as an antigen, immunizes BALB / c mice, uses hybridoma technology, and obtains a hybridoma cell line capable of stably secreting the anti-African swine fever virus pNP868R protein monoclonal antibody through cell fusion, indirect ELISA method screening, limited dilution method subcloning and hybridoma continuous passage culture. The application also obtains an African swine fever virus pNP868R protein monoclonal antibody. The monoclonal antibody has high affinity with the pNP868R protein and can be applied in Western-Blot experiments, indirect immunofluorescence experiments, immunoprecipitation experiments and the capture of proteins interacting with pNP868R, and can be used for the diagnosis, prevention or treatment of African swine fever.

The utility model relates to the technical field of chemiluminescence detection kit, specifically to the chemiluminescence detection kit of Seneca virus VP1 protein monoclonal antibody, including protection subassembly, the inside of the placing box is provided with clamping assembly, the clamping assembly includes the installation strip, the installation strip side surface is provided with sliding slot, the sliding slot quantity is two and the position corresponds, the sliding groove inner wall sliding connection has the slide, the slide quantity is ten and the adjacent two slides are a group, and the fixed connection has the spring between every group two slides, the slide side surface fixed connection has semicircle clamping plate, the semicircle clamping plate quantity is several and the position corresponds to adjacent two semicircle clamping plates, the installation strip side surface fixed connection is in the placing box inner wall, through the elastic clamping adaptation different specifications sampling tube, prevent it from shaking and falling off in the operation, reduce sample spilling, the protection box body can also block spilled sample when accidentally, guarantee detection.

Provided are a monoclonal antibody against a p-Tau217 protein and the use thereof. Specifically, provided is an antibody targeting a p-Tau217 protein or an antigen-binding fragment thereof. The provided antibody can specifically recognize and bind to a p-Tau217 protein without recognizing p-Tau181 and non-phosphorylated Tau proteins, and can be used in the preparation of a detection preparation or kit for diagnosing diseases associated with the p-Tau217 protein, such as Alzheimer's disease.

The present application belongs to the technical field of bioengineering. The present application relates to a recombinant protein, which comprises two dominant antigen epitopes of feline calicivirus (FCV) protein. In order to improve the yield of the recombinant protein in a prokaryotic expression system, the amino acid sequence of the recombinant protein is converted into a corresponding nucleotide sequence by using E. coli preferred codons, the nucleotide sequence is chemically synthesized, and a recombinant expression vector is constructed. The present application also relates to the establishment of a phage library by immunizing mice with the recombinant protein, the screening of a single-chain antibody (scfv) sequence corresponding to the feline calicivirus recombinant protein by panning, the construction of a complete mouse IgG1 antibody sequence expression vector from the obtained scfv sequence, the expression of a monoclonal antibody by instantaneously transferring HEK293F cells, the purification of the monoclonal antibody, and the labeling of europium ions (Eu 3+ ) in the monoclonal antibody, respectively. The optimal monoclonal antibody pairing combination is determined by orthogonal experiments, and the monoclonal antibody can be used for the early diagnosis of upper respiratory tract infection in cats.

The present application relates to the technical field of biology, and particularly discloses a kind of tyrosine iodination modified PP2A protein monoclonal antibody, preparation method and application.The present application is prepared by tyrosine iodination modified PP2A protein antigen peptide, rabbit is immunized to prepare monoclonal antibody, and is selected by phage display technology, expression vector amplification and ELISA screening, finally obtains a strain of monoclonal antibody R004 that can effectively recognize target.The antibody shows good sensitivity and specificity in immunohistochemistry, cell immunofluorescence and protein immunoblotting.The monoclonal antibody screened in the present application can be used in the detection of tyrosine iodination modification of PP2A protein catalytic subunit 307, and provides support for tumor diagnosis, anti-tumor and neurological disease drug research and development.

This application discloses an anti-human IL-17A protein monoclonal antibody, its preparation method, and its application. The anti-human IL-17A protein monoclonal antibody is mouse-derived and has a heavy chain complementarity-determining region as shown in SEQ ID NO. 1-3 and a light chain complementarity-determining region as shown in SEQ ID NO. 4-6. As a labeling antibody, this anti-human IL-17A protein monoclonal antibody can pair with monoclonal antibody 1E9 as a coating antibody to detect human IL-17A. It has high detection sensitivity and specificity and does not cross-react with other cytokine antigens, providing a raw material guarantee for the downstream development of high-quality diagnostic reagents.

The application belongs to the technical field of biology, and discloses a hybridoma cell strain 2F8 secreting a calmodulin 2 monoclonal antibody, a specific monoclonal antibody, and preparation and application. The hybridoma cell strain 2F8 secreting the calmodulin 2 monoclonal antibody is classified and named as 2F8, and is preserved in the China General Microbiological Culture Collection Center on April 9, 2025, with a preservation number of CGMCC No.46343. The application also provides the calmodulin 2 monoclonal antibody secreted by the hybridoma cell strain 2F8 and a polynucleotide encoding the calmodulin 2 monoclonal antibody. The antibody has specific recognition ability for the calmodulin 2, and is suitable for immunological detection, in particular, Western-blot and immunohistochemical detection. The antibody can be used for preparing a reagent, a kit or other immunological detection product for detecting the calmodulin 2, and has practical application value.

The application relates to preparation, epitope identification and application of a PRV TK protein monoclonal antibody, the amino acid sequence of the light chain variable region of the monoclonal antibody is shown as SEQ ID NO. 2, and the amino acid sequence of the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO. 3. Meanwhile, the application provides a PRV TK protein antigen epitope peptide, and the specific amino acid sequence of the PRV TK protein antigen epitope peptide is shown as SEQ ID NO. 4. On the basis of successfully expressing a PRV strain TK protein, the PRV strain TK protein is purified and used as an antigen to immunize mice, and a hybridoma technology is used to prepare a specific monoclonal antibody for the PRV strain TK protein, so as to provide an important tool for TK protein structure and function research of a PRV epidemic strain, and subsequent establishment of a PRV specific diagnosis method and related research.

The application discloses a pancreatic cancer diagnosis marker, and the marker is FAM83A. The application also discloses a kit for detecting FAM83A protein, which comprises effective FAM83A protein, two effective anti-FAM83A protein monoclonal antibodies and matched detection reagents, the two anti-FAM83A protein monoclonal antibodies are monoclonal antibody 1 and monoclonal antibody 2, the heavy chain variable region and light chain variable region sequences of the monoclonal antibody 1 are shown as SEQ ID NO. 1 and SEQ ID NO. 2, and the heavy chain variable region and light chain variable region sequences of the monoclonal antibody 2 are shown as SEQ ID NO. 3 and SEQ ID NO. 4. The kit has the advantages of wide linear range and fast detection speed.

The application discloses an anti-novel coronavirus N protein monoclonal antibody 31A8 and products and applications thereof, and belongs to the fields of cell biology technology and immunology. The light chain of the anti-novel coronavirus N protein monoclonal antibody 31A8 belongs to kappa, the heavy chain belongs to IgG1, the amino acid sequences of three complementarity determining regions (LCDR1-3) of the light chain variable region are respectively shown as SEQ ID No. 1-3, the amino acid sequences of HCDR1-3 of the heavy chain variable region are respectively shown as SEQ ID No. 10-13, and the amino acid sequences of four framework regions (LFR1-4) of the light chain variable region and four framework regions (HFR1-4) of the heavy chain variable region are disclosed. Experiments prove that the monoclonal antibody 31A8 provided in the application has good binding activity with the novel coronavirus, is specific, and can be used for clinically detecting the novel coronavirus SARS-COV-2.

The application discloses preparation and application of anti-Fusobacterium nucleatum PknK and PknL protein monoclonal antibodies and belongs to the technical field of biotechnology. The application takes two virulence proteins PknK and PknL of the Fusobacterium nucleatum as immunogens, successfully prepares monoclonal antibodies against the serine / threonine protein kinases PknK and PknL of the Fusobacterium nucleatum through hybridoma cell fusion technology, and the two monoclonal antibodies against the PknK and PknL proteins of the Fusobacterium nucleatum have good safety, thereby providing a basis for clinical drug safety. More importantly, the antibodies can not only efficiently neutralize bacterial toxins and directly eliminate the pathogenicity of the Fusobacterium nucleatum, but also can be directly combined on the bacterial surface to enhance the recognition and phagocytosis efficiency of immune cells to the bacteria, thereby clearing pathogenic bacteria, and is expected to be developed into a drug for resisting the Fusobacterium nucleatum infection and has a very good application prospect.

This invention discloses an antioxidant low-density lipoprotein monoclonal antibody and its applications, belonging to the field of antibody technology. The antioxidant low-density lipoprotein monoclonal antibody of this invention possesses high specificity, high affinity, and high sensitivity. An immunoturbidimetric assay kit prepared based on the antioxidant low-density lipoprotein monoclonal antibody of this invention can quantitatively detect oxidized low-density lipoprotein in peripheral blood samples, and can have a good diagnostic effect on cardiovascular diseases caused by atherosclerosis (AS).

The application discloses a kind of anti-porcine delta coronavirus (PDCoV) N protein monoclonal antibody and application.The heavy chain variable region of the monoclonal antibody includes CDR1-3 shown in SEQ ID No.1-3;Light chain variable region includes CDR1-3 shown in SEQ ID No.4-6;The monoclonal antibody prepared in the application can recognize PDCoV, and the conservation is good, provides material for the research of PDCoV pathogenesis and for the development of PDCoV diagnostic kit;And can specifically recognize and combine the region of N protein for 313-327aa, provides reliable research tool for exploring the function of N protein;Meanwhile, it can be obtained using conventional genetic engineering or protein engineering method, avoids the loss of antibody in long-term frozen hybridoma cell, also be conducive to antibody optimization on gene and protein level, to improve the specificity and affinity of antibody.

The application provides an anti-human IL-17A protein monoclonal antibody and a preparation method and application thereof. The anti-human IL-17A protein monoclonal antibody is of a murine origin, has a heavy chain complementarity determining region shown as SEQ ID NO. 1-3, and has a light chain complementarity determining region shown as SEQ ID NO. 4-6. The anti-human IL-17A protein monoclonal antibody can be paired with a monoclonal antibody 1C2 as a labeled antibody to detect human IL-17A as a coating antibody, has high detection sensitivity and specificity, does not produce cross-reactions with other cytokine antigens, and provides raw material guarantee for the development of high-quality diagnostic reagents in the downstream.

The application discloses monoclonal antibodies of myxovirus resistance protein A and application thereof, and belongs to the technical field of biology. The application provides monoclonal antibodies MxA-1# and MxA-25# of myxovirus resistance protein A, the monoclonal antibody MxA-1# is produced by a hybridoma cell strain with a preservation number of CGMCC No.46772, the amino acid sequence of a light chain variable region is shown as SEQ ID NO.12, and the amino acid sequence of a heavy chain variable region is shown as SEQ ID NO.17; the monoclonal antibody MxA-25# is produced by a hybridoma cell strain with a preservation number of CGMCC No.46774, the amino acid sequence of a light chain variable region is shown as SEQ ID NO.22, and the amino acid sequence of a heavy chain variable region is shown as SEQ ID NO.26. Both the two antibodies have strong binding capacity with the MxA protein, and lay a foundation for preparing a myxovirus resistance protein A detection kit.

This invention discloses a method for specifically recognizing antigen epitopes. 16 AQTGYAPV 23 This invention relates to a monoclonal antibody D3-mAb against porcine rotavirus and its applications, belonging to the field of antibody bioengineering technology. The monoclonal antibody D3-mAb specifically binds to the VP8 protein of porcine rotavirus and exhibits neutralizing activity; its amino acid sequence is shown in SEQ ID NO. 8. This invention screened a monoclonal antibody D3 with neutralizing activity against the VP8 protein, and subsequently identified the neutralizing antigenic epitope. 16 AQTGYAPV 23 Recognized by D3-mAb, sequence comparison analysis of currently circulating PoRVA strains revealed that this antigenic epitope is absolutely conserved. Furthermore... 16 AQTGYAPV 23 The epitope, located on the surface of the VP8 protein, interacts with the antibody's variable region via six hydrogen bonds. This invention provides a novel approach for designing novel epitope vaccines and antiviral strategies, and the provided monoclonal antibody holds promise for development into a biotherapeutic agent and diagnostic agent for porcine rotavirus infection.

The application provides a kind of anti-RSV N protein monoclonal antibody and its preparation method and application, belong to monoclonal antibody technical field.The heavy chain variable region amino acid sequence of the monoclonal antibody described in the application is as shown in SEQ ID NO.2, the light chain variable region amino acid sequence is as shown in SEQ ID NO.4, is secreted by hybridoma cell strain 1A2, subtype is IgG1, Kappa type, has high affinity and strong specificity to RSV N protein.The antibody can specifically recognize RSV N protein and inactivated RSV A type virus, has high titer, good stability, and can be used for preparing respiratory syncytial virus detection reagent, especially suitable for double antibody sandwich ELISA detection kit, provides core material for early rapid detection of RSV infection, has important clinical application value.

The application discloses a chicken MTRF1L protein monoclonal antibody and application thereof, and belongs to the technical field of biotechnology.The MTRF1L recombinant protein is used for immunizing animals, a sensitized B cell with the ability of secreting specific antibodies and a myeloma cell with unlimited reproduction capacity are fused into a B cell hybridoma through a hybridoma technology, and a chicken MTRF1L protein monoclonal antibody is obtained through screening.The application screens a high-quality nucleotide coding sequence of the MTRF1L gene through bioinformatics analysis, and uses the nucleotide coding sequence as an antigen to immunize BABE mice, a sensitized B cell with the ability of secreting specific MTRF1L antibodies and a myeloma cell with unlimited reproduction capacity are fused into a B cell hybridoma through a hybridoma technology, and a positive hybridoma cell is obtained through screening, and the cell supernatant is used for Western Blot and indirect immunofluorescence detection, and it is found that the monoclonal antibody has good specificity and high sensitivity, which lays a foundation for detection of the MTRF1L protein and research on pathological mechanisms of virus infection diseases.

The application discloses a varicella-zoster virus gL protein monoclonal antibody and application thereof, and relates to the technical field of biological immunity. The varicella-zoster virus gL protein monoclonal antibody comprises CDR-H1 with an amino acid sequence as shown in SEQ ID NO. 9, CDR-H2 with an amino acid sequence as shown in SEQ ID NO. 11, CDR-H3 with an amino acid sequence as shown in SEQ ID NO. 13, CDR-L1 with an amino acid sequence as shown in SEQ ID NO. 16, CDR-L2 with an amino acid sequence of LVS and CDR-L3 with an amino acid sequence as shown in SEQ ID NO. 19. The monoclonal antibody provided by the application can be specifically combined with the varicella-zoster virus gL protein, can be applied to the preparation of products for detecting the varicella-zoster virus gL protein or the varicella-zoster virus gL antibody, and has important application value.

The application discloses a kind of anti-porcine delta coronavirus (PDCoV) N protein monoclonal antibody and application.The heavy chain variable region of the monoclonal antibody includes CDR1-3 shown in SEQ ID No.1-3;Light chain variable region includes CDR1-3 shown in SEQ ID No.4-6;The monoclonal antibody prepared in the application can recognize PDCoV, and the conservation is good, provides material for the research of PDCoV pathogenesis and for the development of PDCoV diagnostic kit;And can specifically recognize and combine the region of N protein 294-307aa, provides reliable research tool for exploring the function of N protein;Meanwhile, it can be obtained using conventional genetic engineering or protein engineering method, avoids the loss of antibody in long-term frozen hybridoma cell, also be conducive to antibody optimization on gene and protein level, to improve the specificity and affinity of antibody.

This invention provides a monoclonal antibody against the Agrin protein and its applications, belonging to the field of immunoassay technology. The monoclonal antibody is secreted by a hybridoma cell line with accession number CCTCC NO: C2025396 and specifically recognizes a key epitope in the Z8 region of the human Agrin protein. Based on this monoclonal antibody and / or the Agrin Z8 peptide, this invention constructs a kit for detecting anti-Agrin Z8 antibodies in the serum of patients with myasthenia gravis. The Agrin monoclonal antibody is used as a positive control or quantitative standard in this kit. Furthermore, this Agrin monoclonal antibody can be used to construct animal models of myasthenia gravis to study the pathogenesis of the disease. The Agrin monoclonal antibody provided by this invention has high specificity and high affinity, providing a key tool for the standardized diagnosis of myasthenia gravis and an effective means for studying its pathogenesis.

This invention belongs to the field of biomedical technology, specifically relating to a kit for detecting brucellosis and its application. The kit includes an enzyme-linked immunosorbent assay (ELISA) plate coated with a capture antibody, HRP-labeled antibody, antigen standards, washing buffer, chromogenic solution, and stop solution. The heavy chain amino acid sequence of the anti-CTSC protein monoclonal antibody CTSC-1 in the capture antibody is shown in SEQ ID NO.1, and the light chain amino acid sequence is shown in SEQ ID NO.2. The heavy chain amino acid sequence of the anti-CTSC protein monoclonal antibody CTSC-2 in the HRP-labeled antibody is shown in SEQ ID NO.3, and the light chain amino acid sequence is shown in SEQ ID NO.4. The kit for detecting brucellosis provided by this invention has high specificity, can specifically detect the content of CTSC protein in urine, has high sensitivity, and has good application prospects.

This invention relates to a humanized Helicobacter pylori cytotoxin-associated protein A monoclonal antibody and its application. The humanized Helicobacter pylori cytotoxin-associated protein A monoclonal antibody includes a heavy chain variable region and a light chain variable region. The nucleic acid sequence of the heavy chain variable region includes CDR1 shown in SEQ ID No. 1 or SEQ ID No. 2, CDR2 shown in SEQ ID No. 3 or SEQ ID No. 4, and CDR3 shown in SEQ ID No. 5 or SEQ ID No. 6. The nucleic acid sequence of the light chain variable region includes CDR1 shown in SEQ ID No. 7 or SEQ ID No. 8, CDR2 with the sequence LVS or KVS, and CDR3 shown in SEQ ID No. 9 or SEQ ID No. 10.

The present application relates to the field of monoclonal antibodies and in vitro diagnostic technology, and in particular to a novel coronavirus N protein monoclonal antibody and a preparation method and application thereof.The present application provides a novel coronavirus N protein monoclonal antibody, which is applied to colloidal gold immunochromatography, can be used for rapid detection of novel coronavirus antigen, has high sensitivity, and the sensitivity can reach 100 pg / ml.

The invention discloses a ferritin antibody pair and application thereof. The ferritin antibody pair comprises a first antibody 7A8 and a second antibody 3H4, amino acid sequences of CDR1, CDR2 and CDR3 of a heavy chain variable region of the first antibody are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; amino acid sequences of CDR1, CDR2 and CDR3 of a light chain variable region of the first antibody are respectively shown as SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6; the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain variable region of the second antibody are respectively as shown in SEQ ID NO: 11, SEQ ID NO: 12 and SEQ ID NO: 13; and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain variable region of the second antibody are respectively shown as SEQ ID NO: 14, SEQ ID NO: 15 and SEQ ID NO: 16. A sandwich method immunofluorescence detection reagent prepared based on the antibody pair shows high sensitivity, wide linear range and high clinical conformity during detection, can meet clinical requirements of primary screening of iron-deficiency anemia, auxiliary diagnosis of tumors and the like, and is suitable for being popularized and used in primary medical treatment and family self-test.

The present application relates to a kind of monoclonal antibody that can identify human PSA antigen, secreting cell strain, its preparation method and its use in immunodetection.The above technical scheme selects the amino acid of 31-124 of PSA protein as antigen peptide, is codon optimized, becomes the gene fragment suitable for expression in escherichia coli BL21, and finally the recombinant protein includes TRX protein label, PSA protein fragment and histidine protein label.The recombinant protein is immunized to mouse, is fused by cell, screening and subcloning, obtains the mouse hybridoma cell strain 16F1 of high-efficiency secreted anti-PSA protein monoclonal antibody and the anti-PSA protein monoclonal antibody secreted by the cell strain.The antibody obtained in the present application has high specificity, sensitivity, and can specifically recognize the cell expressing PSA protein, is suitable for immunological detection, especially immunohistochemical detection.

This invention discloses a monoclonal paired antibody against cardiac myosin-binding protein C and its application. The specific antibody comprises a first antibody and / or a second antibody; the first antibody and the second antibody bind to different epitopes of cardiac myosin-binding protein C, and the similarity between the heavy chain CDR3 sequence of the first antibody and the heavy chain CDR3 sequence of the second antibody is <70%, and the similarity between the light chain CDR3 sequence of the first antibody and the light chain CDR3 sequence of the second antibody is <70%. The monoclonal paired antibody against cardiac myosin-binding protein C of this invention exhibits high specificity and high affinity, with a cross-reactivity rate of <1% with skeletal muscle myosin-binding protein. The kit of this invention has a detection sensitivity ≤5 pg / mL and a detection time of only 10-15 min, making it suitable for the ultra-early diagnosis of acute myocardial infarction.

The application belongs to the technical field of biological detection, and discloses an anti-SpCas9 protein monoclonal antibody, a detection kit and application, wherein the heavy chain variable region amino acid sequence of the anti-SpCas9 protein monoclonal antibody is shown as SEQ ID NO. 1, and the light chain variable region amino acid sequence is shown as SEQ ID NO. 2; and a double-antibody sandwich ELISA detection kit is constructed by taking the monoclonal antibody as a capture antibody and an anti-SpCas9 protein polyclonal antibody as a detection antibody. The ELISA detection kit has the advantages of high sensitivity, strong specificity, good repeatability, high-throughput and rapid detection, can accurately quantify the expression and residual amount of SpCas9 protein in a gene editing biological sample, and provides strong support for the application and supervision of CRISPR / Cas9 gene editing technology.

The application discloses an anti-UreB protein monoclonal antibody and a preparation method and application thereof, and relates to the technical field of monoclonal antibodies.The anti-UreB protein monoclonal antibody comprises a heavy chain variable region and a light chain variable region, wherein the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO.1, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO.5.The anti-UreB protein monoclonal antibody has excellent affinity effect on UreB protein, is used in combination with an anti-UreB protein antibody (8-B5-E6 antibody), can specifically recognize non-overlapping epitopes of the UreB protein, has no steric hindrance, and has a binding activity reaching an ng / mL level, can be used for double-antibody sandwich method detection, and has an excellent application prospect in the field of Helicobacter pylori detection.