191 results about "Protein.monoclonal" patented technology

The invention is applicable to the technical field of C-reactive protein detection, and provides an anti-C-reactive protein monoclonal antibody idiotype antibody, a preparation method and application, and the anti-C-reactive protein monoclonal antibody idiotype antibody is secreted by a hybridoma cell strain; the hybridoma cell strain is a 7C4 hybridoma cell strain; the preparation method of the anti-C-reactive protein monoclonal antibody idiotype antibody comprises the following steps: S1, obtaining hybridoma cells: preparing an anti-C-reactive protein monoclonal antibody Fab fragment, then carrying out animal immunization, then carrying out cell fusion, and finally screening and cloning the hybridoma cells; and S2, preparing and screening an anti-C reactive protein monoclonal antibody idiotype antibody. The anti-C-reactive-protein monoclonal antibody idiotype antibody is used for replacing C-reactive protein to serve as a labeled antibody, the labeling process is simple, the labeling efficiency is high, and meanwhile the situation that a final product is affected due to instability of the C-reactive protein is avoided. Experimental results show that the detection method is good in specificity and wide in linear range.

The invention provides monoclonal antibodies of H protein and N protein of canine distemper virus and application thereof in preparation of a fluorescent microsphere antigen detection test strip. The monoclonal antibody disclosed by the invention is characterized in that (1) the monoclonal antibody contains a heavy chain variable region CDV-N-3A11-VH and a light chain variable region CDV-N-3A11-VL; the amino acid sequence of the CDV-N-3A11-VH is as shown in the 1st to 116th sites of SEQ ID No.1 in a sequence table; the amino acid sequence of the CDV-N-3A11-VL is as shown in the 1st site to the 113th site of SEQ ID No.2 in a sequence table. (2) a heavy chain variable region CDV-H-1G5-VH and a light chain variable region CDV-H-1G5-VL are contained; the amino acid sequence of the CDV-H-1G5-VH is as shown in the first site to the 114 site of SEQ ID No.3 in a sequence table; the amino acid sequence of the CDV-H-1G5-VL is as shown in the 1st site to the 1st site and the 1st site to the 1st site of SEQ ID No.4 in a sequence table. The canine distemper virus fluorescent microsphere antigen test strip composed of the two antibodies is suitable for detecting canine distemper viruses in samples such as eye and nose swabs and virus cultures, is high in sensitivity and good in specificity, and can be used for early diagnosis of canine distemper virus infection.

The invention discloses an antigen detection kit developed based on a human metapneumovirus F protein monoclonal antibody and application of the antigen detection kit, and belongs to the technical field of antibodies. The monoclonal antibody is a monoclonal antibody 1 and / or a monoclonal antibody 2; the amino acid sequence of a light chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.6, and the amino acid sequence of a heavy chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.8; the amino acid sequence of the light chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.2, and the amino acid sequence of the heavy chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.4. The kit containing the two monoclonal antibodies is used for detecting the metapneumovirus, has the characteristics of high sensitivity and high specificity, and can realize rapid detection and auxiliary diagnosis of HMPV infection. Therefore, the monoclonal antibody for resisting the F protein of the HMPV and the detection kit of the monoclonal antibody have wide application prospects and auxiliary diagnosis values.

The invention relates to the technical field of antibodies, in particular to an anti-African swine fever virus p30 protein monoclonal antibody and application thereof, and the anti-African swine fever virus p30 protein monoclonal antibody can be specifically combined with African swine fever virus p30 protein. The anti-ASFV p30 protein monoclonal antibody is prepared by immunizing a BALB / c mouse through an immunological method, and the antibody is high in specificity, titer and affinity and can be applied to immunological detection or antibody detection kits.

The invention discloses a hybridoma cell strain secreting an anti-micro-nano plastic monoclonal antibody and application thereof.The hybridoma cell strain comprises a hybridoma cell strain MP-4 and a hybridoma cell strain MP-9, the hybridoma cell strain MP-4 and the hybridoma cell strain MP-9 are obtained through self screening of the inventor, the preservation number of the hybridoma cell strain MP-4 is CCTCC NO: C2024262, the preservation number of the hybridoma cell strain MP-9 is CCTCC NO: C2024262, and the preservation number of the hybridoma cell strain MP-9 is CCTCC NO: C2024262. The preservation number of the hybridoma cell strain MP-9 is CCTCC NO: C2024261. The passage cell strain of the hybridoma cell provided by the invention can stably secrete an anti-broad-spectrum micro-nano plastic protein monoclonal antibody, the secreted antibody has high titer and good sensitivity, can simultaneously recognize PS, PVC, PP, PE and PET, has good broad-spectrum recognizability, provides a core raw material for establishing a broad-spectrum immunodetection method of micro-plastics, and has good application prospects. Wide application prospects are realized.

The invention discloses a porcine delta coronavirus spike protein monoclonal antibody, an antigen epitope peptide and application, and belongs to the technical field of biology. The antibody comprises a light chain variable region and a heavy chain variable region, the amino acid sequence of the light chain variable region is as shown in SEQ ID No.1, and the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No.3. According to the invention, a highly conservative linear B cell epitope (the amino acid sequence is DFGEARLD) of a PDCoV spike protein receptor binding domain (S-RBD) and a neutralizing monoclonal antibody capable of being specifically bound to the epitope are identified for the first time. The epitope peptide and the monoclonal antibody provided by the invention can be used for immunological detection and serological investigation of the PDCoV.

The invention discloses a porcine epidemic diarrhea virus S protein monoclonal antibody and application thereof. Specifically, two monoclonal antibodies GC42G2-1 and GC33D8-1 which have broad-spectrum binding characteristics with the porcine epidemic diarrhea virus S protein are obtained firstly, the GC42G2-1 antibody has good universality and can have strong affinity with different strains, and the GC33D8-1 antibody can be used for binding the porcine epidemic diarrhea virus S protein in a broad-spectrum manner. The GC42G2-1 can be independently used, and also can be matched with a polyclonal antibody or GC33D8-1 to be used for sandwich ELISA (enzyme-linked immunosorbent assay) so as to detect and quantify PEDV (porcine epidemic diarrhea virus). Secondly, the two monoclonal antibodies are respectively used as a coating antibody and a detection antibody, a double-antibody sandwich ELISA method is established, and the double-antibody sandwich ELISA method is applied to PEDV vaccine S protein quantification, has better parallelism compared with other antibodies, and can be widely applied to detection of different strains. The method disclosed by the invention has the advantages of high sensitivity, high accuracy, high repeatability and high flux, and can be used for the development of blocking ELISA (Enzyme-Linked Immunosorbent Assay), capturing ELISA, indirect ELISA, competitive ELISA and detection of porcine epidemic diarrhea viruses by colloidal gold test strips.

The invention discloses a monoclonal antibody aiming at humanized cellular prion protein and application of the monoclonal antibody. The invention relates to the technical field of biology, in particular to a monoclonal antibody aiming at humanized cellular prion protein and application of the monoclonal antibody. The antibody is a monoclonal antibody or an antigen binding fragment thereof, and comprises a heavy chain variable region and a light chain variable region, amino acid sequences of LCDR1, LCDR2 and LCDR3 in a light chain variable region of the antibody are sequentially shown as the 24 to 40 , the 56 to 62 and the 95 to 103 of SEQ ID No.1; amino acid sequences of HCDR1, HCDR2 and HCDR3 in a heavy chain variable region of the antibody are sequentially shown as the 26th site to the 32nd site, the 52nd site to the 57th site and the 99th site to the 103th site of SEQ ID No.2. The antibody disclosed by the invention has relatively high affinity with the cellular prion protein, and can be applied to detection or diagnosis of tumors expressing the cellular prion protein.

The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody as well as a hybridoma cell strain and application thereof, and belongs to the technical field of immune globulin. The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody and also provides a hybridoma cell strain OTI10F11, the hybridoma cell strain OTI10F11 is preserved in the China General Microbiological Culture Collection Center on April 26, 2025, and the preservation number of the hybridoma cell strain OTI10F11 is No.46356. The invention further provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody. The hybridoma cell strain OTI10F11 provided by the invention can stably secrete an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody, and can be specifically combined with H < + > / K < + > ATPase beta protein, so that the specificity, accuracy and reliability of immunodetection are remarkably improved, and the hybridoma cell strain OTI10F11 can be suitable for marking H < + > / K < + > ATPase beta protein in tissue cells.

The invention belongs to the technical field of biology, and discloses a hybridoma cell strain 2F8 capable of secreting a calmodulin 2 monoclonal antibody, a specific monoclonal antibody, and preparation and application of the specific monoclonal antibody. The hybridoma cell strain secreting the calmodulin 2 monoclonal antibody, provided by the invention, is classified and named as 2F8, and is preserved in the China General Microbiological Culture Collection Center on April 9, 2025, and the preservation number is CGMCC No.46343. The invention also provides a calmodulin 2 monoclonal antibody secreted by the calmodulin 2 monoclonal antibody and polynucleotide for coding the calmodulin 2 monoclonal antibody. The antibody disclosed by the invention has specific recognition capability on calmodulin 2, and is suitable for immunological detection, especially Western-blot and immunohistochemical detection. The method can be used for preparing reagents, kits or other immunological detection products for detecting calmodulin 2, and has practical application value.

The invention provides an influenza B virus NP protein monoclonal antibody 23E2 and application thereof, the sequences of heavy chain variable regions CDR1, CDR2 and CDR3 of the influenza B virus NP protein monoclonal antibody 23E2 are respectively shown as SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8; the sequences of the light chain variable regions CDR1, CDR2 and CDR3 are respectively as shown in SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12. The antibody is high in affinity and good in specificity.

The invention discloses an anti-rabies virus N protein monoclonal antibody, a detection reagent and application of the anti-rabies virus N protein monoclonal antibody. The monoclonal antibody for resisting the rabies virus N protein comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody with relatively high affinity and detection sensitivity for resisting rabies virus N protein lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

The present invention provides a monoclonal antibody against the p-Tau217 protein and its applications. Specifically, the present invention provides an antibody targeting the p-Tau217 protein or an antigen-binding fragment thereof. The antibody provided by the present invention can specifically recognize and bind to the p-Tau217 protein, and does not recognize p-Tau181 and non-phosphorylated Tau protein, and can be used to prepare detection preparations or kits for diagnosing p-Tau217 protein-related diseases, such as Alzheimer's disease.

The invention discloses an antibody for detecting porcine acute diarrhea syndrome coronavirus, a fluorescence immunochromatography test strip and application of the antibody and the fluorescence immunochromatography test strip, and aims to overcome the defects that an existing SADS-CoV detection technology is low in sensitivity, complex to operate and incapable of being applied on site. The method comprises the following steps: screening high-specificity anti-S1 protein monoclonal antibodies (such as 1B8 and 3C9); a quantum dot labeled antibody is combined with a chromatographic structure containing a T line (S1 protein) and a C line (SPA protein); the kit provided by the invention has the advantages that by optimizing the formula of the buffer solution, the technical effects of high-sensitivity detection (100 TCID50 / mL), on-site rapid visual interpretation and zero cross reaction are realized, the technical bottleneck of on-site rapid and accurate diagnosis is overcome, the operation is simple and convenient, and the kit is suitable for on-site screening of clinical samples.

The application belongs to the technical field of biology and particularly relates to an African swine fever virus pNP868R protein monoclonal antibody and a preparation method and application thereof. The application takes the pNP868R protein prepared by eukaryotic expression as an antigen, immunizes BALB / c mice, uses hybridoma technology, and obtains a hybridoma cell line capable of stably secreting the anti-African swine fever virus pNP868R protein monoclonal antibody through cell fusion, indirect ELISA method screening, limited dilution method subcloning and hybridoma continuous passage culture. The application also obtains an African swine fever virus pNP868R protein monoclonal antibody. The monoclonal antibody has high affinity with the pNP868R protein and can be applied in Western-Blot experiments, indirect immunofluorescence experiments, immunoprecipitation experiments and the capture of proteins interacting with pNP868R, and can be used for the diagnosis, prevention or treatment of African swine fever.

The present invention relates to methods, uses, and compositions for the treatment of cancer (e.g., a breast cancer or a pancreatic cancer). More specifically, the invention concerns the treatment of patients having cancer for the therapeutic inhibition of cancer cell growth and metastasis with an anti-Cadherin 11 monoclonal antibody with specific monoclonal antibody clones 23C6 or 3H10.

The invention provides a preparation method of a monoclonal antibody of coccidian oocyst protein, and belongs to the technical field of antibodies. The method comprises the following steps: carrying out flow sorting on single antigen-specific B lymphocytes, carrying out stimulated differentiation culture in a specific culture medium by taking EL4B5 as feeder layer cells, cracking obtained plasma cells, carrying out PCR (Polymerase Chain Reaction) amplification to obtain a variable region sequence of a monoclonal antibody, and carrying out recombinant expression to obtain the coccidian oocyst protein monoclonal antibody. The monoclonal antibody of the coccidian oocyst protein provided by the invention comprises at least one of coccidian monoclonal antibodies 6B10, 5C8 and 5D5. According to the present invention, the antigen affinity verification is performed on the monoclonal antibodies obtained through the recombinant expression, and the results show that the three monoclonal antibodies have good antigen affinity and good detection specificity, such that the effective tool is provided for the subsequent diagnosis and detection of the porcine coccidiosis and the porcine coccidiosis infection diseases.

The present disclosure relates to human antibodies specific to dermatomyositis antigen peptides, preparation methods and uses. Specifically, the present disclosure relates to a monoclonal antibody that specifically binds to MDA5 antigen protein, a preparation method of the monoclonal antibody and a use of the aforementioned monoclonal antibody in the preparation of a drug for the diagnosis, prevention or treatment of inflammatory myopathy or its complications. The antibody or antigen-binding fragment against MDA5 screened by the present disclosure specifically binds to MDA5 antigen, can be used as a reference standard for qualitative detection of MDA5 positivity, realizes qualitative or quantitative detection of the level of anti-MDA5 autoantibody in patients with inflammatory myopathy or its complications, and has important significance for the clinical diagnosis, disease monitoring and treatment of patients with DM, CAMD, DM-ILD, CADM-ILD and the like.

The application relates to a monoclonal antibody capable of identifying a human TROP2 antigen, a preparation method thereof and the use thereof in immunodetection. The application provides an anti-TROP2 protein rabbit monoclonal antibody, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is an amino acid sequence shown in SEQ ID NO. 1, and the amino acid sequence of a light chain variable region of the monoclonal antibody is an amino acid sequence shown in SEQ ID NO. 2. The antibody has high specificity and sensitivity, can specifically identify cells expressing TROP2 protein, and is suitable for immunological detection, in particular, immunohistochemical detection.

The invention relates to preparation, epitope identification and application of a PRV TK protein monoclonal antibody. The amino acid sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO.2, and the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.3. Meanwhile, the invention provides the PRV TK protein antigen epitope peptide, and the specific amino acid sequence of the PRV TK protein antigen epitope peptide is as shown in SEQ ID NO. 4. According to the research, on the basis that the PRV strain TK protein is successfully expressed, the PRV strain TK protein is purified to serve as an antigen to immunize a mouse, the specific monoclonal antibody aiming at the PRV strain TK protein is prepared by utilizing a hybridoma technology, and an important tool is provided for PRV epidemic strain TK protein structure and function research and subsequent establishment of a PRV specific diagnosis method and related research.

The invention discloses a yellow fever virus envelope protein monoclonal antibody and application thereof, and belongs to the technical field of medicines. Yellow fever virus envelope protein E expressed by human embryo kidney 293 cells is used as an antigen to immunize a rabbit, B cells capable of being specifically combined with the yellow fever virus envelope protein E are screened from rabbit spleen cells through flow sorting, and signal peptide and variable region gene fragments of an antibody are cloned through reverse transcription-polymerase chain reaction. According to the present invention, the monoclonal antibody with high neutralizing activity and capable of completely protecting mice from yellow fever virus lethal attack is obtained by using the monoclonal antibody as a template, connecting the monoclonal antibody and the constant region gene to an expression vector, carrying out mammalian cell expression and purification, and carrying out enzyme-linked immunosorbent assay, in-vitro virus neutralization and mouse virus attack experiment, such that the monoclonal antibody has high neutralizing activity and can completely protect mice from yellow fever virus lethal attack; the monoclonal antibody has application value in prevention and treatment of yellow fever.

The present application belongs to the technical field of antibody preparation, and belongs to a human chitinase-3-like protein 1 monoclonal antibody 1A9, a recombinant carrier, a recombinant cell and a method and application. The present application provides a monoclonal antibody 1A9 capable of specifically recognizing CHI3L1, wherein the monoclonal antibody 1A9 comprises a heavy chain and a light chain; a variable region of the heavy chain comprises three heavy chain complementarity determining regions, and amino acid sequences of the three heavy chain complementarity determining regions are shown as SEQ ID NO. 1 to SEQ ID NO. 3; a light chain variable region of the monoclonal antibody 1A9 comprises three light chain complementarity determining regions, and amino acid sequences are shown as SEQ ID NO. 4 to SEQ ID NO. 6. The monoclonal antibody has good titer, and the expression carrier and the recombinant cell for preparing the monoclonal antibody are easy to store and modify.

The invention discloses a feline leukemia virus p27 protein monoclonal antibody, a hybridoma cell strain and application thereof, and relates to the technical field of biology, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.1 in a sequence table, and the amino acid sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO.2 in the sequence table. According to the invention, a cell fusion technology is utilized to establish a hybridoma cell strain p27-3A5 capable of secreting a p27 protein monoclonal antibody, and a specific monoclonal antibody 3A5 is obtained. And the minimum linear epitope identified by the antibody is identified, so that the establishment of subsequent preparation of a diagnostic kit such as a colloidal gold test strip is facilitated.

The present invention belongs to the field of antibody technology, and in particular relates to monoclonal antibodies, antibody conjugates and applications thereof against p21 proteins. The amino acid sequences of the complementary determining regions CDR1-3 on the light chain variable region of the monoclonal antibody are shown as SEQ ID NO.3-5, respectively; the amino acid sequences of the complementary determining regions CDR1-3 on the heavy chain variable region are shown as SEQ ID NO.8-10, respectively. The monoclonal antibody provided by the present invention can specifically recognize p21 proteins expressed by human cells and tissues, has good affinity for binding to p21 proteins, high recognition sensitivity, and strong anti-interference ability, is suitable for immunodiagnostic analysis of p21 proteins in tissues / cells, and has broad application prospects and good market prospects in clinical diagnosis and scientific research detection. In addition, the antibody has cross-reactivity to homologous p21 proteins of humans, rats and mice, and has certain applicability to the detection of rat and mouse p21 proteins.

An anti-CD79B protein monoclonal antibody and related products and uses thereof are disclosed. The anti-CD79B protein monoclonal antibody comprises a heavy chain and a light chain, wherein the heavy chain includes a heavy chain variable region, and the light chain includes a light chain variable region. The heavy chain variable region includes complementary-determining regions CDR1, CDR2, and CDR3 with amino acid sequences as shown in SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3, respectively. The light chain variable region comprises complementary-determining regions CDR1, CDR2, and CDR3 with amino acid sequences as shown in SEQ ID NO:9, SEQ ID NO:10, and SEQ ID NO:11, respectively. The anti-CD79B protein monoclonal antibody exhibits high specificity and sensitivity, enabling specific recognition of cells expressing CD79B protein. It is suitable for immunological detection, and effectively prevents false-positive and false-negative results in immunological assays, thereby further improving the accuracy of detection and diagnostic outcomes.

The present invention discloses a porcine delta-coronavirus double-antibody sandwich ELISA detection kit based on S protein monoclonal antibody, its use method and application. The PDCoV S protein was expressed using the ExpiCHO expression system, and then the purified S protein was used as an immunogen to immunize mice to obtain monoclonal antibodies. Then, a double-antibody sandwich ELISA kit was established using monoclonal antibody 3D7 and HRP-labeled MAb 9G4 as capture and detection antibodies, respectively. The kit has good sensitivity, with a detection limit of 0.12 ng / mL for purified S protein and a detection limit of 1.96×103 copies / μL for PDCoV. Specificity analysis showed that the kit had no cross-reactivity with other porcine enteric coronaviruses PEDV, TGEV and PoRV that are similar to PDCoV in clinical symptoms, and the consistency with the RT-PCR method reached 91.03%, with a Kappa value of 0.814, indicating the reliability of the kit for clinical sample detection. In addition, the kit can also quantitatively evaluate the content of S protein in PDCoV inactivated vaccine and subunit vaccine, providing a powerful tool for evaluating these vaccines.

The application provides an influenza B virus NP protein monoclonal antibody 10G11 and application thereof, wherein the heavy chain variable region CDR1, CDR2 and CDR3 of the influenza B virus NP protein monoclonal antibody 10G11 have sequences as shown in SEQ ID NO:6, SEQ ID NO:7 and SEQ ID NO:8 respectively; and the light chain variable region CDR1, CDR2 and CDR3 have sequences as shown in SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:12 respectively. The antibody has high affinity and good specificity.

The invention provides an anti-PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) Nsp4 protein monoclonal antibody with an inhibition effect and application thereof, a hybridoma cell strain capable of stably secreting the anti-Nsp4 protein monoclonal antibody is prepared, and a new antigen epitope is identified in nsp4 through expressed overlapping truncated protein. Wherein the 123ITEAGELV130 identified by the monoclonal antibody 1C11 shows the potential of universally identifying different types of PRRSV (Porcine Reproductive and Respiratory Syndrome Virus). Meanwhile, expression of Nsp4 protein can be detected in the early stage of PRRSV infection by utilizing the monoclonal antibody 1C11, the monoclonal antibody has a blocking effect on PRRSV positive serum, and it is found that the monoclonal antibody 1C11 can inhibit proliferation of PRRSV in vitro. Research results increase the understanding of PRRSV Nsp4 protein structure and antigen characteristics, and provide a basis for PRRSV early antigen detection.

The present application relates to a kind of monoclonal antibody that can identify human Topo II alpha antigen, secretes cell strain, its preparation method and its use in immunodetection.The above technical solution selects the amino acid of 1301-1531 of Topo II alpha protein as antigen peptide, codon optimization becomes the gene fragment suitable for expression in escherichia coli BL21, and finally the recombinant protein includes Topo II alpha protein fragment and histidine protein label.The recombinant protein is immunized to mouse, is fused by cell, screening and subcloning, and obtains mouse hybridoma cell strain, and the anti Topo II alpha protein monoclonal antibody secreted by the cell strain is obtained.The antibody obtained by the present application has high specificity, sensitivity, and can specifically recognize the cell expressing Topo II alpha protein, and is suitable for immunological detection, especially immunohistochemical detection.

The present invention provides monoclonal antibodies against the cat allergen FeI d 1 protein and their applications. First, the present invention obtained two hybridoma cell lines, 2A7 and 6B1, that stably secrete FeI d 1 monoclonal antibodies. Furthermore, a double-antibody sandwich ELISA method was established using 2A7 as the capture antibody and biotin-labeled 6B1 as the detection antibody. This method can be used to monitor FeI d 1 protein antigen levels, replacing existing indirect ELISA detection methods. It exhibits high specificity and sensitivity, is simple to operate, and produces clear results.