300 results about "Protein.monoclonal" patented technology

The invention relates to an HPV16 type E6 oncoprotein monoclonal antibody as well as a preparation method and application thereof. The monoclonal antibody specifically bound with the HPV16E6 recombinant antigen is obtained through screening, the monoclonal antibody is applied to a biotin-avidin amplification ELISA system, the E6 oncoprotein of HPV16 can be specifically detected, the sensitivity is 100 pg / mL, the monoclonal antibody does not intersect with oncoprotein of other high-risk HPV, and the E6 oncoprotein of HPV16 can be specifically detected. High specificity and sensitivity are shown for cervical exfoliated cell sample detection of normal people, CIN3 people and cervical cancer people, and the method can be applied to early screening of cervical cancer in the future; compared with traditional exfoliative cytology examination, the method is faster and more accurate, meanwhile, the risk of false positive and misdiagnosis caused by too high sensitivity of nucleic acid detection can be avoided, and the method has very high application value.

The invention discloses a monoclonal antibody for distinguishing a hog cholera lapinized virus vaccine strain and a hog cholera lapinized virus epidemic strain and application of the monoclonal antibody, and belongs to the field of immune globulin. The E2 protein monoclonal antibody or the antigen binding fragment thereof comprises a heavy chain variable region (SEQ ID No.1) and a light chain variable region (SEQ ID No.3). The invention also discloses an ELISA method for identifying and distinguishing hog cholera lapinized virus vaccine strains and epidemic strains by using the E2 protein monoclonal antibody. The monoclonal antibody disclosed by the invention has a specific reaction with the swine fever virus epidemic strain and does not react with the swine fever lapinized virus vaccine strain, and the swine fever lapinized virus vaccine strain and the epidemic strain can be accurately identified and distinguished by utilizing an ELISA competition method established by the monoclonal antibody. The monoclonal antibody provided by the invention can be used in the fields of CSFV immunization, serological diagnosis of wild viruses, immune effect evaluation, related experiments and the like, and provides antibody resources for effective detection and purification of CSFV.

The invention belongs to biotechnology, and particularly relates to an African swine fever virus p30 protein monoclonal antibody as well as a preparation method and application thereof. The main technology comprises the following steps: immunizing a BALB / c mouse by using a recombinant p30 protein prepared by prokaryotic expression as an antigen, and carrying out cell fusion, indirect ELISA method screening, limited dilution method subcloning and hybridoma continuous subculture by using a hybridoma technology to obtain a hybridoma cell line capable of stably secreting an anti-African swine fever virus p30 protein monoclonal antibody. The invention also discloses a sequence of an immunogen of the prepared monoclonal antibody, a subtype of the monoclonal antibody, a CDR sequence, a preservation number of a hybridoma cell strain and application of the monoclonal antibody in a Western Blot experiment, an indirect immunofluorescence experiment, a neutralization experiment and a blocking ELISA method for detecting the African swine fever virus antibody in swine serum.

The invention provides a monoclonal antibody of a p-Tau217 protein and an application of the monoclonal antibody. In particular, the present invention provides an antibody or an antigen binding fragment thereof targeting a p-Tau217 protein. The antibody provided by the invention can specifically recognize and bind the p-Tau217 protein, does not recognize p-Tau181 and non-phosphorylated Tau protein, and can be used for preparing a detection preparation or a kit for diagnosing diseases related to the p-Tau217 protein, such as Alzheimer's disease.

The invention discloses monoclonal antibodies NP5 and NP8 for resisting influenza A virus NP protein and application of the monoclonal antibodies NP5 and NP8, and belongs to the technical field of monoclonal antibodies. According to the invention, 19 influenza A virus NP protein monoclonal antibodies are prepared, and the monoclonal antibodies NP5 and NP8 are obtained by screening through ELISA (enzyme-linked immuno sorbent assay) detection and sequencing analysis. The NP5 comprises a light chain CDR2 of which the amino acid sequence is RTS, light chains CDR1 and CDR3 of which the amino acid sequences are shown as SEQ ID NO.3-4, and heavy chains CDR1-3 of which the amino acid sequences are shown as SEQ ID NO.7-9; the NP8 comprises a light chain CDR2 of which the amino acid sequence is LVS, light chains CDR1 and CDR3 of which the amino acid sequences are as shown in SEQ ID NO.12 to SEQ ID NO.13, and heavy chains CDR1-3 of which the amino acid sequences are as shown in SEQ ID NO.16 to SEQ ID NO.18. The invention lays a foundation for preparing an influenza A virus detection kit.

The present invention belongs to the field of biological detection technology, and specifically relates to an anti-monkey C-reactive protein monoclonal antibody, an enzyme-linked immunosorbent assay kit and its application. Named CRP-13, CRP-13 includes a heavy chain variable region and a light chain variable region; the heavy chain variable region has three complementary determining regions CDR-H1 to CDR-H3, and the amino acid sequences are shown in SEQ ID NO.6-SEQ ID NO.8; the light chain variable region has three complementary determining regions CDR-L1 to CDR-L3, and the amino acid sequences are shown in SEQ ID NO.9-SEQ ID NO.11. The monoclonal antibody of the present invention can specifically bind to monkey C-reactive protein with high specificity. The above monoclonal antibody can be used to screen a paired monoclonal antibody, and a monkey C-reactive protein enzyme-linked immunosorbent assay kit is obtained based on the combination of the monoclonal antibodies, which can detect the content of monkey C-reactive protein in monkey serum, plasma or tissue fluid.

The invention discloses an anti-norovirus monoclonal antibody and application thereof, and discloses an anti-VP1 protein monoclonal antibody, complementarity determining regions HCDR1, HCDR2 and HCDR3 of a heavy chain variable region of the monoclonal antibody are as shown in SEQ ID NO: 1-3, complementarity determining regions LCDR1 and LCDR3 of a light chain variable region of the monoclonal antibody are as shown in SEQ ID NO: 4-5, and the amino acid sequence of the complementarity determining region LCDR2 of the light chain variable region is NAK. The anti-norovirus monoclonal antibody disclosed by the invention has strong affinity and high binding activity with norovirus type 1 VP1 protein, and is an excellent monoclonal antibody for detecting and treating norovirus.

The invention belongs to the technical field of biology, and particularly relates to a latex duplex immunochromatography test strip for rapidly identifying a porcine transmissible gastroenteritis virus (TGEV) and a porcine epidemic diarrhea virus (PEDV) and a preparation method of the latex duplex immunochromatography test strip. Soluble TGEV N protein and PEDV N protein with immunogenicity and reactivity are respectively obtained by utilizing a prokaryotic expression system, the soluble TGEV N protein and the soluble PEDV N protein are used as immunogenicity to immunize mice, and specific monoclonal antibodies of the TGEV N protein and the PEDV N protein are respectively obtained by utilizing cell fusion and subcloning screening technologies. The antibody variable region gene sequence is obtained by using a nested PCR amplification technology, and can be subsequently obtained by using a gene engineering or protein engineering method. Then, the latex duplex immunochromatography test strip for detecting TGEV and PEDV, which is convenient, rapid and high in sensitivity, and the preparation method are researched and developed on the basis of specific monoclonal antibodies of TGEV N protein and PEDV N protein.

The invention is applicable to the technical field of C-reactive protein detection, and provides an anti-C-reactive protein monoclonal antibody idiotype antibody, a preparation method and application, and the anti-C-reactive protein monoclonal antibody idiotype antibody is secreted by a hybridoma cell strain; the hybridoma cell strain is a 7C4 hybridoma cell strain; the preparation method of the anti-C-reactive protein monoclonal antibody idiotype antibody comprises the following steps: S1, obtaining hybridoma cells: preparing an anti-C-reactive protein monoclonal antibody Fab fragment, then carrying out animal immunization, then carrying out cell fusion, and finally screening and cloning the hybridoma cells; and S2, preparing and screening an anti-C reactive protein monoclonal antibody idiotype antibody. The anti-C-reactive-protein monoclonal antibody idiotype antibody is used for replacing C-reactive protein to serve as a labeled antibody, the labeling process is simple, the labeling efficiency is high, and meanwhile the situation that a final product is affected due to instability of the C-reactive protein is avoided. Experimental results show that the detection method is good in specificity and wide in linear range.

The invention belongs to the technical field of biological medicines, and particularly relates to application of umbilical cord mesenchymal stem cells and monoclonal antibodies in combined treatment of cancers. The monoclonal antibody is a monoclonal antibody specifically aiming at human PAFAH1B3 protein, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO: 1, and the amino acid sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO: 2. The PAFAH1B3 protein monoclonal antibody can significantly inhibit the proliferation capacity of pancreatic cancer cells, and after the umbilical cord mesenchymal stem cells and the PAFAH1B3 protein monoclonal antibody are combined for use, the inhibition effect on the proliferation capacity of the pancreatic cancer cells is better, and the umbilical cord mesenchymal stem cells can be used for preparing anti-cancer drugs and have good application prospects.

The invention provides monoclonal antibodies of H protein and N protein of canine distemper virus and application thereof in preparation of a fluorescent microsphere antigen detection test strip. The monoclonal antibody disclosed by the invention is characterized in that (1) the monoclonal antibody contains a heavy chain variable region CDV-N-3A11-VH and a light chain variable region CDV-N-3A11-VL; the amino acid sequence of the CDV-N-3A11-VH is as shown in the 1st to 116th sites of SEQ ID No.1 in a sequence table; the amino acid sequence of the CDV-N-3A11-VL is as shown in the 1st site to the 113th site of SEQ ID No.2 in a sequence table. (2) a heavy chain variable region CDV-H-1G5-VH and a light chain variable region CDV-H-1G5-VL are contained; the amino acid sequence of the CDV-H-1G5-VH is as shown in the first site to the 114 site of SEQ ID No.3 in a sequence table; the amino acid sequence of the CDV-H-1G5-VL is as shown in the 1st site to the 1st site and the 1st site to the 1st site of SEQ ID No.4 in a sequence table. The canine distemper virus fluorescent microsphere antigen test strip composed of the two antibodies is suitable for detecting canine distemper viruses in samples such as eye and nose swabs and virus cultures, is high in sensitivity and good in specificity, and can be used for early diagnosis of canine distemper virus infection.

The invention discloses a hybridoma cell strain capable of recognizing 188th amino acid of a highly pathogenic serum type 4 fowl adenovirus Hexon protein, a monoclonal antibody and application of the hybridoma cell strain. The preservation number of the cell strain 2C5 is CGMCC NO.45829, and the monoclonal antibody 2C5 capable of specifically recognizing the highly pathogenic serum type 4 fowl adenovirus hexon Hexon protein is secreted. The amino acid sequence of the antigen epitope recognized by the gene is Hexon protein GPGRNP, wherein the R188 amino acid in the Hexon protein is the key amino acid recognized by the gene. The monoclonal antibody 2C5 prepared by the invention has the specificity of high-pathogenicity FAdV-4, can specifically recognize the high-pathogenicity FAdV-4, has no cross reaction with non-pathogenicity FAdV-4 and FAdV-4 of other serotypes, and can be used as a diagnostic reagent for distinguishing the high-pathogenicity FAdV-4 and the non-pathogenicity FAdV-4.

The invention discloses an antigen detection kit developed based on a human metapneumovirus F protein monoclonal antibody and application of the antigen detection kit, and belongs to the technical field of antibodies. The monoclonal antibody is a monoclonal antibody 1 and / or a monoclonal antibody 2; the amino acid sequence of a light chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.6, and the amino acid sequence of a heavy chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.8; the amino acid sequence of the light chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.2, and the amino acid sequence of the heavy chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.4. The kit containing the two monoclonal antibodies is used for detecting the metapneumovirus, has the characteristics of high sensitivity and high specificity, and can realize rapid detection and auxiliary diagnosis of HMPV infection. Therefore, the monoclonal antibody for resisting the F protein of the HMPV and the detection kit of the monoclonal antibody have wide application prospects and auxiliary diagnosis values.

The invention belongs to the technical field of biology, and relates to a latex immunochromatography test strip for rapidly detecting porcine epidemic diarrhea virus (PEDV) and a preparation method of the latex immunochromatography test strip. The test strip comprises a PVC (polyvinyl chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, a quality control line C and a detection line T are arranged on the nitrocellulose membrane. A latex microsphere labeled PEDV N protein monoclonal capture antibody is sprayed on the combination pad, a detection line T is coated with a PEDV N protein monoclonal detection antibody 1E1, and a quality control line C is coated with goat anti-mouse IgG. The test strip can specifically recognize PEDV, and does not react with other porcine intestinal coronaviruses with similar clinical symptoms. The test strip is good in specificity, relatively high in sensitivity and simple to operate, a result is interpreted within 10 minutes, and compared with a traditional colloidal gold detection technology, the stability and the color development effect are obviously improved.

The invention provides a hybridoma cell strain secreting a grass carp reovirus VP4 protein monoclonal antibody as well as the monoclonal antibody and application thereof, and belongs to the technical field of aquatic reovirus detection. The hybridoma cell strain is named as GCRV-VP4-6E7B12, and is preserved in the China Center for Type Culture Collection on December 10, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2024160. The grass carp reovirus VP4 protein monoclonal antibody 6E7B12 secreted by the hybridoma cell strain GCRV-VP4-6E7B12 has the advantage of being specifically combined with an antigen. The enzyme-labeled monoclonal antibody is used as a detection antibody, the VP4 protein polyclonal antibody is used as a capture antibody, the content of the grass carp reovirus VP4 protein in a sample to be detected can be quantitatively detected by adopting a double-antibody sandwich ELISA method, the detection range is 1-32 ng / mL, and the lower detection limit is 1 ng / mL. The detection method has the advantages of high sensitivity and wide detection range.

The invention discloses a type 2 GAstV capsid protein monoclonal antibody and an antigen epitope peptide of the type 2 GAstV capsid protein monoclonal antibody. The monoclonal antibody is prepared by immunizing a mouse with capsid protein GAstV Cap of the type 2 goose astrovirus as an antigen, and is named as 1F1; the monoclonal antibody 1F1 comprises a heavy chain variable region and a light chain variable region, and the amino acid sequences of the heavy chain variable region and the light chain variable region are sequentially shown as SEQ ID No.3 and SEQ ID No.5. In order to determine the B cell antigen epitope of the screened monoclonal antibody, the invention also constructs a GAstV Cap truncated fusion protein, and the antigen recognition epitope is determined through indirect ELISA and Western blotting tests, so that screening is carried out step by step until the shortest linear epitope recognized by the monoclonal antibody is determined, and the amino acid sequence of the shortest linear epitope is shown as SEQ ID No.10. Therefore, the monoclonal antibody and the antigen epitope of the monoclonal antibody can be applied to research on structures and functions of GAstV capsid proteins and preparation of serological diagnostic tools and epitope vaccines.

The invention relates to the technical field of antibodies, in particular to an anti-African swine fever virus p30 protein monoclonal antibody and application thereof, and the anti-African swine fever virus p30 protein monoclonal antibody can be specifically combined with African swine fever virus p30 protein. The anti-ASFV p30 protein monoclonal antibody is prepared by immunizing a BALB / c mouse through an immunological method, and the antibody is high in specificity, titer and affinity and can be applied to immunological detection or antibody detection kits.

The invention discloses a hybridoma cell strain secreting an anti-micro-nano plastic monoclonal antibody and application thereof.The hybridoma cell strain comprises a hybridoma cell strain MP-4 and a hybridoma cell strain MP-9, the hybridoma cell strain MP-4 and the hybridoma cell strain MP-9 are obtained through self screening of the inventor, the preservation number of the hybridoma cell strain MP-4 is CCTCC NO: C2024262, the preservation number of the hybridoma cell strain MP-9 is CCTCC NO: C2024262, and the preservation number of the hybridoma cell strain MP-9 is CCTCC NO: C2024262. The preservation number of the hybridoma cell strain MP-9 is CCTCC NO: C2024261. The passage cell strain of the hybridoma cell provided by the invention can stably secrete an anti-broad-spectrum micro-nano plastic protein monoclonal antibody, the secreted antibody has high titer and good sensitivity, can simultaneously recognize PS, PVC, PP, PE and PET, has good broad-spectrum recognizability, provides a core raw material for establishing a broad-spectrum immunodetection method of micro-plastics, and has good application prospects. Wide application prospects are realized.

The invention provides a monoclonal antibody of a p-Tau181 protein and an application of the monoclonal antibody. Specifically, the invention provides an antibody targeting p-Tau181 protein or an antigen binding fragment of the antibody. The antibody provided by the invention can specifically recognize and bind the p-Tau181 protein, does not recognize p-Tau217 and non-phosphorylated Tau protein, and can be used for preparing a detection preparation or a kit for diagnosing diseases related to the p-Tau181 protein, such as Alzheimer's disease.

The monoclonal antibody of the glycoprotein Gn of the severe fever with thrombocytopenia syndrome virus is screened on the basis of immune repertoire sequencing, and the biological characteristics of the monoclonal antibody are identified. The method comprises the following steps: taking purified SFTSV-Gn as bait, taking virus specific B cells from an immunized female BALB / c mouse B cell bank, selecting an antibody sequence with potential binding activity by utilizing a 10 * immune repertoire sequencing method in combination with bioinformatics analysis, constructing the antibody sequence to an eukaryotic expression vector pcDNA3.4, expressing and purifying a monoclonal antibody, and identifying the biological activity of the monoclonal antibody. 14 antibodies are selected from 3544 captured B cells, functional verification shows that 12 antibodies can be combined with SFTSV-Gn glycoprotein, and the Gn-5 antibody and the Gn-7 antibody have high combining capacity; western blot, indirect immunofluorescence test and flow cytometry prove that the antibody can specifically recognize the SFTSV, the effective concentration is as low as 2 mu g / mL, and a foundation is laid for development of a rapid diagnosis reagent for the SFTSV.

The prealbumin and C-reactive protein combined detection kit comprises a detection card, an IC card, a sample adding hole, a card reading window and a sample diluent, the detection card comprises a prealbumin detection reagent strip, a C-reactive protein detection reagent strip and a card shell for loading the prealbumin detection reagent strip and the C-reactive protein detection reagent strip; each of the prealbumin detection reagent strip and the C-reactive protein detection reagent strip comprises a reaction bottom plate, a sample pad, a combination pad, a chromatography membrane and absorbent paper, and a prealbumin monoclonal antibody marked by fluorescent microspheres or a C-reactive protein monoclonal antibody marked by fluorescent microspheres and DNP-BSA marked by fluorescent microspheres are correspondingly adopted; a quality control line and a detection line are arranged on the chromatography membrane; the sample adding holes comprise a prealbumin sample adding hole and a C reactive protein sample adding hole; the sample diluent is used for diluting a whole blood or serum sample and comprises a prealbumin sample diluent and a C-reactive protein sample diluent. Corresponding methods of preparation and methods of use are also disclosed.

The invention discloses a porcine delta-coronavirus double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) detection kit based on an S protein monoclonal antibody as well as a use method and application of the porcine delta-coronavirus double-antibody sandwich ELISA detection kit. A PDCoV S protein is expressed by adopting an ExpiCHO expression system, then the purified S protein is used as an immunogen to immunize a mouse, and the monoclonal antibody is obtained. And then, respectively using the monoclonal antibody 3D7 and the MAb 9G4 labeled by the HRP as capture and detection antibodies, and establishing the double-antibody sandwich ELISA kit. The kit has good sensitivity, the detection limit of purified S protein is 0.12 ng / mL, and the detection limit of PDCoV is 1.96 * 10 < 3 > copies / [mu] L. Specificity analysis shows that the kit has no cross reactivity with other porcine intestinal coronaviruses PEDV, TGEV and PoRV with clinical symptoms similar to PDCoV, the consistency with an RT-PCR (reverse transcription-polymerase chain reaction) method reaches 91.03%, and the Kappa value is 0.814, so that the reliability of the kit for clinical sample detection is shown. In addition, the kit can also quantitatively evaluate the content of S protein in PDCoV inactivated vaccines and subunit vaccines, and provides a powerful tool for evaluating the vaccines.

The invention belongs to the technical field of antibodies, and discloses a monoclonal antibody for recognizing EB (Epstein-Barr) virus gp350 protein and application of the monoclonal antibody. The invention provides an anti-EB virus gp350 protein monoclonal antibody or an antigen binding fragment thereof, the anti-EB virus gp350 protein monoclonal antibody comprises 1A12 and 2E4, the 1A12 has high affinity for the gp350 protein (KD (M) = 3.415 E-10), the 2E4 has high affinity for the gp350 protein (KD (M) = 4.851 E-9), the two monoclonal antibodies or the antigen binding fragments thereof can significantly inhibit infection of EBV on epithelial cells and B cells, and the anti-EB virus gp350 protein monoclonal antibody or the antigen binding fragment thereof can be used for preparing anti-EB virus gp350 protein anti-EB virus gp350 protein anti-EB virus gp350 protein anti-EB virus gp350 protein anti-EB virus gp350 protein anti-EB virus. The kit can be used for detecting the existence or level of the gp350 protein in a sample, detecting EB virus, diagnosing diseases caused by EB virus infection, preventing EB virus infection and / or treating and / or preventing diseases caused by EB virus infection.

The invention discloses a porcine delta coronavirus spike protein monoclonal antibody, an antigen epitope peptide and application, and belongs to the technical field of biology. The antibody comprises a light chain variable region and a heavy chain variable region, the amino acid sequence of the light chain variable region is as shown in SEQ ID No.1, and the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No.3. According to the invention, a highly conservative linear B cell epitope (the amino acid sequence is DFGEARLD) of a PDCoV spike protein receptor binding domain (S-RBD) and a neutralizing monoclonal antibody capable of being specifically bound to the epitope are identified for the first time. The epitope peptide and the monoclonal antibody provided by the invention can be used for immunological detection and serological investigation of the PDCoV.

The invention relates to the technical field of immune globulin, in particular to an anti-human BCOR protein monoclonal antibody and a hybridoma cell strain and application thereof. The invention provides an anti-human BCOR protein monoclonal antibody which is secreted by a hybridoma cell strain OTI3D11, the hybridoma cell strain OTI3D11 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 28, 2024, and the preservation number of the hybridoma cell strain OTI3D11 is CGMCC No.46244. The anti-human BCOR protein monoclonal antibody can be used for preparing anti-human BCOR protein. The anti-human BCOR protein monoclonal antibody secreted by the hybridoma cell strain OTI3D11 disclosed by the invention has very high specificity and sensitivity. Therefore, the anti-BCOR monoclonal antibody secreted by the hybridoma cell strain OTI3D11 can be used for immunohistochemical detection so as to detect the expression condition of related tumor BCOR, and a basis is provided for diagnosis of patients by clinical doctors.

On one hand, the invention discloses the monoclonal antibody for resisting the porcine circovirus type 3 Cap protein, the monoclonal antibody for resisting the porcine circovirus type 3 Cap protein is a monoclonal antibody P3, and amino acid sequences of a heavy chain variable region and a light chain variable region of the monoclonal antibody P3 are respectively shown as SEQ ID NO.3 and SEQ ID NO.4. The invention also discloses a preparation method of the monoclonal antibody for resisting the porcine circovirus type 3 Cap protein. The invention also discloses a porcine circovirus type 3 antibody detection kit. The kit comprises the monoclonal antibody P3, the porcine circovirus type 3 Cap protein and a matched detection reagent. The monoclonal antibody P3 has the advantages of good specificity and good affinity, and can be used for detection of the porcine circovirus type 3 antibody and development of related detection products. Meanwhile, the kit provided by the invention has good specificity and sensitivity, and is suitable for large-scale detection of the porcine circovirus type 3 antibody.

The invention discloses a porcine epidemic diarrhea virus S protein monoclonal antibody and application thereof. Specifically, two monoclonal antibodies GC42G2-1 and GC33D8-1 which have broad-spectrum binding characteristics with the porcine epidemic diarrhea virus S protein are obtained firstly, the GC42G2-1 antibody has good universality and can have strong affinity with different strains, and the GC33D8-1 antibody can be used for binding the porcine epidemic diarrhea virus S protein in a broad-spectrum manner. The GC42G2-1 can be independently used, and also can be matched with a polyclonal antibody or GC33D8-1 to be used for sandwich ELISA (enzyme-linked immunosorbent assay) so as to detect and quantify PEDV (porcine epidemic diarrhea virus). Secondly, the two monoclonal antibodies are respectively used as a coating antibody and a detection antibody, a double-antibody sandwich ELISA method is established, and the double-antibody sandwich ELISA method is applied to PEDV vaccine S protein quantification, has better parallelism compared with other antibodies, and can be widely applied to detection of different strains. The method disclosed by the invention has the advantages of high sensitivity, high accuracy, high repeatability and high flux, and can be used for the development of blocking ELISA (Enzyme-Linked Immunosorbent Assay), capturing ELISA, indirect ELISA, competitive ELISA and detection of porcine epidemic diarrhea viruses by colloidal gold test strips.

The invention discloses a monoclonal antibody aiming at humanized cellular prion protein and application of the monoclonal antibody. The invention relates to the technical field of biology, in particular to a monoclonal antibody aiming at humanized cellular prion protein and application of the monoclonal antibody. The antibody is a monoclonal antibody or an antigen binding fragment thereof, and comprises a heavy chain variable region and a light chain variable region, amino acid sequences of LCDR1, LCDR2 and LCDR3 in a light chain variable region of the antibody are sequentially shown as the 24 to 40 , the 56 to 62 and the 95 to 103 of SEQ ID No.1; amino acid sequences of HCDR1, HCDR2 and HCDR3 in a heavy chain variable region of the antibody are sequentially shown as the 26th site to the 32nd site, the 52nd site to the 57th site and the 99th site to the 103th site of SEQ ID No.2. The antibody disclosed by the invention has relatively high affinity with the cellular prion protein, and can be applied to detection or diagnosis of tumors expressing the cellular prion protein.

The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody as well as a hybridoma cell strain and application thereof, and belongs to the technical field of immune globulin. The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody and also provides a hybridoma cell strain OTI10F11, the hybridoma cell strain OTI10F11 is preserved in the China General Microbiological Culture Collection Center on April 26, 2025, and the preservation number of the hybridoma cell strain OTI10F11 is No.46356. The invention further provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody. The hybridoma cell strain OTI10F11 provided by the invention can stably secrete an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody, and can be specifically combined with H < + > / K < + > ATPase beta protein, so that the specificity, accuracy and reliability of immunodetection are remarkably improved, and the hybridoma cell strain OTI10F11 can be suitable for marking H < + > / K < + > ATPase beta protein in tissue cells.

The invention belongs to the technical field of biology, and discloses a hybridoma cell strain 2F8 capable of secreting a calmodulin 2 monoclonal antibody, a specific monoclonal antibody, and preparation and application of the specific monoclonal antibody. The hybridoma cell strain secreting the calmodulin 2 monoclonal antibody, provided by the invention, is classified and named as 2F8, and is preserved in the China General Microbiological Culture Collection Center on April 9, 2025, and the preservation number is CGMCC No.46343. The invention also provides a calmodulin 2 monoclonal antibody secreted by the calmodulin 2 monoclonal antibody and polynucleotide for coding the calmodulin 2 monoclonal antibody. The antibody disclosed by the invention has specific recognition capability on calmodulin 2, and is suitable for immunological detection, especially Western-blot and immunohistochemical detection. The method can be used for preparing reagents, kits or other immunological detection products for detecting calmodulin 2, and has practical application value.