Kit for simultaneously detecting retinol-binding protein (RBP) in urine sample and serum sample

Abstract

The invention provides a kit for simultaneously detecting RBP in a urine sample and a serum sample. The kit comprises a reagent R1 and a reagent R2, the reagent R1 is a polymer buffer solution having an agglomeration promotion effect, the reagent R2 is a buffer solution containing a latex-antibody crosslink, and the latex-antibody crosslink comprises anti-RBP polyclonal antibody marked large latex particles and anti-RBP monoclonal antibody marked small latex particles. The kit which adopts a composite monoclonal and polyclonal antibody sensitized latex enhanced immune detection method has the advantages of simultaneous satisfying of the requirements comprising good specificity, high sensitivity and wide linear range, small antibody application amount, and cost reduction, and can be simultaneously used for the clinic detection of the RBP in human blood and urine.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a kit capable of simultaneously detecting retinol-binding protein in urine and serum samples. Background technique [0002] Retinol-Binding protein (RBP) is a transporter of retinol (vitamin A) in the blood, involved in the transport of retinol / acid in serum and cells, it is mainly synthesized in the liver, and It is released into the blood and enters various tissues. By interacting with retinol, prealbumin and cell surface receptors, it plays an important role in the storage, metabolism and transport of vitamin A to surrounding target organs. In the blood, RBP mainly exists in the form of complexes bound by retinol and prealbumin. When retinol in the complex binds to target cells, RBP is separated from prealbumin and filtered out from the glomerulus. End renal tubular epithelial cells absorb and degrade. RBP dysfunction will lead to abnormal storage, transport, distributio...

AI Technical Summary

Problems solved by technology

However, due to the use of a single polyclonal antibody, the common immunoturbidimetric method has the characteristics of good specificity but poor sensitivity or good sensitivity but poor specificity, which cannot meet the requirements of specificity and sensitivity at the same time.

Among them, the website of the State Intellectual Property Office of China discloses a double-antibody composite retinol-binding protein detection kit, which uses a mixture of monoclonal antibodies and polyclonal antibodies for common immunoturbidimetric methods, which can meet the requirements of specificity and sensitivity at the same time. However, the general immunoturbidimetric method requires a large amount of antibodies, and the cost of monoclonal antibodies is high, which limits its clinical application.

The website of the State Intellectual Property Office of China also discloses a detection kit based on latex particle-coated retinol-binding protein, which adopts the polyclonal antibody latex enhancement method, which greatly reduces the amount of antibody and reduces the cost, but its detection linear range is relatively low. Narrow, cannot be used for the determination of RBP in serum and RBP in urine at the same time

A double-antibody latex-enhanced retinol-binding protein detection kit is also disclosed on the website of the State Intellectual Property Office of China. Two monoclonal antibody latex-enhanced methods are used. The linear range is wide, but the sensitivity is low, and it still cannot be used simultaneously. Determination of RBP in serum and RBP in urine

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