716 results about "Urine sample" patented technology

The present invention provides a method for evaluating urinary system diseases based on urine detection, which belongs to the technical field of urology. The method for evaluating urinary system diseases based on urine detection comprises: adding corresponding reagents to three urine samples in sequence according to a preset time point sequence, and obtaining urine sample images after each addition of the reagents; performing image preprocessing on each acquired urine sample image, extracting image features including color changes, sediment morphology and distribution; establishing a time series feature data set, arranging and combining the image features corresponding to each time point in time sequence; using a pre-trained image recognition model to analyze the image features in the time series feature data set, and obtaining feature vectors; the method can solve the problems of high examination cost, great trauma, long examination time, limited diagnostic accuracy, etc. that the existing means of diagnosing urinary system diseases generally have.

The ureter and urine bag comprises a urine bag body, a spiral pipe, a connecting pipe, a threaded connector pipe and a urine guiding pipe, the threaded connector pipe comprises a pipe body and an insertion pipe, internal threads with the thread pitch being 1.8-2.2 mm are machined on the inner wall of the pipe body, external threads matched with the internal threads are machined on the outer wall of the insertion pipe, and the spiral pipe is connected with the connecting pipe. Two groups of wing plates are symmetrically arranged on the outer wall of the cannula to provide rotating acting points, and a collecting assembly is integrated on the side wall of the urine bag and used for aseptically collecting urine samples. Traditional plug-pull type connection is replaced by a precise thread fit structure, a clear rotating force application point is provided for medical staff, operation is labor-saving and convenient, connector pollution is avoided, the urinary tract infection risk is effectively reduced, and the nursing comfort of a patient is improved.

The invention discloses a nucleic acid aptamer for accurate molecular typing of multi-parameter bladder cancer cells and application of the nucleic acid aptamer, and belongs to the technical field of biology. The technical problem to be solved in the invention is how to realize early accurate diagnosis of bladder cancer and molecular typing of bladder cancer in clinic. In order to solve the technical problem, the invention comprises nucleic acid aptamers including nucleic acid aptamers Seq12a, BCfsw1-3a, BCfsw1-4a, BCfsw1-10a, BCfsw2-19a and BCfsw2-19a, and a composition composed of the nucleic acid aptamers. According to the application, nucleic acid aptamer screening is carried out aiming at different levels of bladder cancer cells and bladder cancer clinical urine samples to obtain nucleic acid aptamer groups covering information of different types of bladder cancer, and cell molecular typing is realized on bladder cancer cells and normal cell lines; and the kit is preliminarily applied to clinical urine sample molecular typing and bladder cancer recurrence course monitoring.

The invention discloses a method for detecting the contents of VMA, HVA and 5-HIAA in urine by using liquid chromatography-tandem mass spectrometry. The method comprises the following steps: S1, removing VMA, HVA and 5-HIAA in urine to obtain a blank urine matrix; s2, weighing VMA, HVA and 5-HIAA solid powder, fixing the volume with methanol, mixing the dissolved liquids, diluting with methanol-water to obtain a VMA, HVA and 5-HIAA working solution, and finally diluting step by step with a blank urine matrix to obtain a calibration product; s3, respectively taking a urine sample to be detected, the calibration substance and the quality control substance, adding the mixed internal standard working solution of VMA, HVA and 5-HIAA isotopes, uniformly mixing, centrifuging, and taking supernate; s4, separating the supernate by using liquid chromatography; s5, the separated supernate enters a mass spectrum to be detected, and the content of VMA, HVA and 5-HIAA in the urine sample to be detected is obtained. The detection method disclosed by the invention is simple and rapid in pretreatment, high in specificity, low in reagent consumption cost, free of environmental pollution, small in required sample size, easy in high-throughput operation and suitable for being developed into a kit for conventional clinical detection.

The invention discloses a method for rapidly detecting common candida of urinary tract infection based on a nucleic acid mass spectrometry technology. The method comprises the following steps: extracting total nucleic acid from a urine sample of a patient; amplifying the extracted total nucleic acid by adopting a PCR (Polymerase Chain Reaction) technology to obtain an amplification product containing a target sequence; analyzing a characteristic peak map of the amplification product containing the target sequence by using a single-base extension primer and a matrix-assisted laser desorption / ionization time flight mass spectrometry technology; and determining whether specific candida exists in the sample according to the characteristic peak map. According to the invention, a set of detection system suitable for common candida is obtained through sequence screening and optimization of the amplification primers and the single-base extension primers, five common candida of UTI are detected based on the system, and a nucleic acid mass spectrometry technology is combined, so that the result can be clearly distinguished, a complex traditional detection means is avoided, and the detection efficiency is improved.

The invention provides a diabetic nephropathy early warning method, system, equipment and medium, and relates to the technical field of medical data analysis and prediction.The method comprises the steps that multiple urine samples of a user are collected, target urine protein detection is conducted, and multiple urine protein concentrations are obtained; each urine protein concentration is combined with a normal urine protein concentration set to be input into an abnormal data recognition tree, the number of layers of multiple recognition nodes is obtained, and the detection confidence coefficient is obtained through calculation; configuring an abnormal proportion according to the detection confidence coefficient, inputting a plurality of urine protein concentration combination normal urine protein concentration sets into an abnormal data identification tree, deleting urine protein concentrations divided into abnormal data in the abnormal proportion before, and obtaining screened urine protein concentrations; according to the screened urine protein concentration, an auxiliary early warning coefficient is obtained through calculation, and early warning is carried out. The technical problem of low early warning accuracy of diabetic nephropathy in the prior art is solved.

Disclosed here are kits comprising pre-packed stabilizing solutions for stabilizing combinations of biomarkers demonstrating sufficient accuracy and specificity for identifying kidney injuries. Such kits can be better adapted for sample collection at a subject's dwelling, thus easing the burdensome requirement of continuous monitoring for kidney injury.

The invention discloses a urine sampling device for the endocrinology department and a sampling method, and belongs to the technical field of medical analysis. Comprising the following modules: an automatic sampling control module for realizing automatic control of the whole urine sampling process, ensuring that sampling accords with a standard process, and supporting automatic sub-packaging and storage management of samples; the sample quality control module is used for monitoring the physical characteristics of the samples in real time, automatically evaluating the effectiveness of the samples in combination with environmental parameter monitoring, and identifying and marking abnormal samples; the data acquisition and processing module is used for comprehensively acquiring physiological data, nutrition metabolism data, operation process data and sample tracing information, and performing cleaning, standardization and fusion processing to generate a standardized data set; the intelligent diagnosis and analysis module is used for intelligently detecting abnormal physiological indexes and carrying out disease risk prediction and early warning; and the man-machine interaction module provides a visual operation interface and integrates multi-level user authority management, automatic report generation and remote monitoring.

Doubly labeled water with enhanced protocol. One embodiment is a method including providing, to a user, a doubly labeled water (DLW) dose for the user to ingest, the DLW dose including deuterium and oxygen-18, wherein an amount of the deuterium is less than 0.12 grams per kilogram (g / kg) of body water of the user, and wherein an amount of the oxygen-18 is less than 0.18 g / kg of body water of the user. The method also includes receiving, from the user, a non-cooled shipment of urine samples collected in connection with ingestion of the DLW dose, wherein the urine samples remain uncooled after collection and during transit for a period of up to 24 days. The method further includes processing the urine samples with a liquid water isotope analyzer to determine one or more metabolic parameters of the user.

The invention relates to methods, devices, and software for distinguishing hemoglobin (Hb) from myoglobin (Mb) in urine samples using peroxidase-reactive test strips. A time-resolved video of the color change on the test pad is recorded, and hue values are extracted and analyzed over time. Diagnostic differentiation between Hb and Mb is achieved by evaluating the hue-time curve, in particular via endpoint hue values and the ratio of integrated hue values in distinct time intervals. The invention further comprises a standardized optical setup for recording, a software-based evaluation algorithm, and a refillable cartridge for in-situ urine analysis devices containing appropriate test fields. The invention enables combined testing with additional urine parameters and is suitable for medical and non-clinical applications such as sport and home diagnostics.

The invention relates to the field of environmental chemistry and biological sample analysis, in particular to an analysis method for detecting oxidative stress biomarkers in urine. The analysis method capable of simultaneously detecting dityrosine, 8-hydroxyguanosine, 8-hydroxydeoxyguanosine and 4-hydroxynonenal mercaptouric acid in human urine is provided on the basis of liquid chromatography-tandem mass spectrometry, effective purification of a urine sample is achieved through specific pretreatment, the matrix effect is reduced, and the detection sensitivity is improved. And specific liquid chromatogram parameter conditions are combined, so that simultaneous detection of the four oxidative stress biomarkers is realized. The method is good in selectivity, high in sensitivity and high in detection speed, the problem that polar and non-polar components cannot be measured at the same time at present is solved, the contained oxidative stress biomarkers are more comprehensive and more reasonable, and the oxidative stress damage condition of the organism can be better reflected.

There is a provided a urine collection device for collecting an uncontaminated urine sample from a pre-continent or an incontinent user, the urine collection device comprising: a receptacle configured to be placed within a nappy or an incontinence pad of the user to receive and retain a urine sample collected from the user in use, wherein the receptacle comprises: a receiving portion for receiving the urine sample, the receiving portion comprising an opening configured to engage with a human body portion surrounding external genitalia of the user, thereby enabling urine exiting the body of the user to be captured directly in use; a reservoir for retaining the received urine sample in use; and an outlet for draining the retained urine sample in use, wherein the opening is larger than the outlet.

An intermittent catheter package including a plurality of components and a catheterization tray configured to facilitate a catheterization procedure. The plurality of components include a urinary catheter fluidly connected to a urine-drainage bag and a sampling-port access device configured to fluidly connect to a urine-sampling port of the urinary catheter or the urine-drainage bag for aseptic collection of one or more urine samples.The catheterization tray includes at least a first compartment, a second compartment, a third compartment, and a fourth compartment. The second compartment is connected to the third compartment Step-by-step surface instructions for performing the catheterization procedure are incorporated into the catheterization tray.

The invention relates to the field of medical treatment, in particular to application, a model and a method of a protein fragment of urine regulatory protein in preparation of a kidney disease early detection product, and the amino acid sequence of the protein fragment of the urine regulatory protein is any shown in SEQ ID NO.1-8. The model constructed according to the protein fragment of the urine regulatory protein can be used as a non-invasive tool for early detection and monitoring of kidney diseases, and by analyzing the unique mode of the protein fragment existing in a urine sample, the model which indicates renal insufficiency, renal function and renal function can be identified before the traditional clinical biomarker is abnormal. Therefore, intervention can be carried out earlier.

The present invention provides a kit and a detection method for synchronously detecting catecholamines and their metabolites, which relates to the technical field of analytical detection. The kit contains a diluent, a solid-phase extraction reagent, an LC-MS / MS detection reagent, a solid-phase extraction device, and a solid-phase extraction packing material. Among them, the solid-phase extraction packing material creatively adopts zwitterionic exchange microspheres containing both carboxyl and quaternary ammonium groups. The kit can not only synchronously and efficiently separate and detect 8 kinds of catecholamines and their metabolites in urine samples, but also separate and detect 6 kinds of amino-containing catecholamines and their metabolites in blood samples. When the above kit is used to separate and detect catecholamines and their metabolites, the extraction recovery rate is relatively high, and the linear range covers the reference value range of normal detection, which can significantly shorten the detection time of urine samples, improve the detection efficiency of mass spectrometry, and is conducive to popularization and application in clinical detection.

A disposable diaper product useful for collecting a urine sample from an infant is disclosed. In one example the product may have a liquid control structure overlying a liquid impermeable backsheet, with a plan surface area defining a volume coextensive therewith, wherein a portion of the volume defined by at least 50 percent of the plan surface area contains no more than 50 percent by weight absorbent material. In another example the product may have a liquid control structure overlying a liquid impermeable backsheet, and have an average Liquid Release Ratio of at least 3 percent. A method for collecting a urine sample from an infant is also disclosed, in which a diaper is used for collection.

1. Name of the design product: Women's urine sampling container. 2. Use of the design product: For women's urine sampling. 3. Design key points of the design product: Lies in the shape. 4. Picture or photo that best shows the design key points: Perspective view.

The invention discloses a urine collection and storage device, and relates to the technical field of urine collection devices, the urine collection and storage device comprises a base frame for receiving urine and a liquid guide channel fixedly arranged on the base frame, the base frame is provided with a floating collection mechanism for quantitatively collecting urine at the middle end, and the floating collection mechanism comprises a horizontal base fixedly connected to the inner wall of the base frame; the horizontal base is provided with a horizontal assembly for adjusting the relative position of the transverse base and the liquid guide channel, the two horizontal sides of the middle clamping base are provided with a main inner cavity column and a slave inner cavity column respectively, and the partition frame is provided with a blocking assembly for partitioning and sealing the internal space of the main inner cavity column and the slave inner cavity column. By arranging the floating collection mechanism, midstream urine of a patient can be accurately collected, and meanwhile, tissues and impurities deposited at the bottom of a urine bag are abandoned, so that the purity and representativeness of a urine sample are effectively improved, the accuracy of a detection result is ensured, and the misdiagnosis risk caused by sample pollution or impurity mixing is reduced.

The invention discloses a culture method of high-activity human urine-derived stem cells, which relates to the technical field of biological medicine, and is technically characterized by comprising the following steps: collecting urine samples of pregnant and lying-in women, coating a culture plate with gelatin, inducing adherence, performing passage amplification, performing immunofluorescence identification, performing function detection and other optimized conditions, and separating and culturing the stem cells. Compared with urine-derived stem cells of normal adults, the stem cells derived from pregnant women have stronger activity, higher dryness and faster multiplication capacity. The invention provides a novel and superior cell source for tissue engineering and regenerative medicine.

The invention discloses a urine sampling device for a clinical laboratory, relates to the technical field of urine sampling and detection, and aims to solve the technical problem that middle-section urine samples are difficult to collect due to physiological degeneration of old people, and the urine sampling device comprises a receiving cup, a collecting and filtering mechanism, a cutting assembly, an extrusion unit and a backflow unit. In the aspect of urine filtration, the collecting and filtering mechanism works cooperatively by means of the bearing cylinder, the circulating cylinder and other components, particles in urine are filtered accurately, and the particles are prevented from interfering with medical urine detection; the front section of urine is accurately intercepted and impurities are removed through the intercepting assembly; when the urine volume is insufficient, the extrusion unit and the backflow unit are tacitly matched to automatically supplement the urine sample volume, in addition, the urine flowing form can be optimized by arranging a cone in the circulation channel, all the assemblies are easy, convenient and flexible to operate, the overall structure is stable and good in sealing performance, and various medical urine detection requirements can be met; a high-quality urine sample is provided, and clinical examination work is powerfully supported.

The invention discloses a metabolite detection method for diagnosing lithangiuria, and relates to the technical field of metabolite detection. The method comprises the following steps: collecting and preprocessing a urine sample, and generating a composite fluorescence signal based on the preprocessed urine sample so as to obtain a composite function; constructing an orbital function system, and performing projection processing on a composite function to obtain initial orbital energy; based on the initial orbital energy, a mutual exclusion function between metabolites is introduced, an effective energy contribution value is calculated, and a decoupled energy response amount is obtained; converting the decoupled energy response quantity into a concentration estimation value, and generating a rotation response value based on the concentration estimation value; generating a coupling threshold based on the concentration estimation value and the rotation response value; and carrying out risk scoring based on the coupling threshold value to obtain an individual risk score value of the urinary calculi. The invention solves the problems of inaccurate concentration estimation of the metabolite and low metabolite detection precision caused by inaccurate analysis of the metabolite under complex interference conditions in the existing metabolite detection method.

A method for characterizing disease activity in a systemic lupus erythematosus patient (SLE), comprising obtaining a dataset associated with a blood, serum, plasma or urine sample from the patient, assessing the dataset for a presence or an amount of protein expression of at least one innate serum or plasma mediator, assessing the dataset for a presence or an amount of protein expression of at least one adaptive serum or plasma mediator biomarker, assessing the dataset for a presence or an amount of at least one chemokine / adhesion molecule biomarker, assessing the dataset for a presence or an amount of at least one soluble TNF superfamily biomarker, assessing the dataset for a presence or an amount of at least one inflammatory mediator biomarker, assessing the dataset for a presence or an amount at least one SLE-associated autoantibody specificity biomarker and calculating a Lupus Disease Activity Immune Index (LDAII) score.

According to an aspect of the present invention, there is provided there is provided a dog pee pad, comprising: two or more layers; pulp dispersed between the layers; a pH indicator; and a polyethylene bottom. According to another aspect of the present invention, there is provided a method, comprising: of assessing the health of a dog, comprising: training a dog to pee on a pee pad; obtaining a sample of dog urine on the pee pad; waiting for the reaction of the sample with compounds in the pee pad; comparing the color indicated by the reaction with a color scale; and correlating the color scale to a chart of indicated health conditions.

The invention relates to the technical field of medical detection, in particular to an endocrine hypertension screening method based on immunological analysis, which comprises the following steps: collecting a random urine sample, removing interferents by using a composite nano adsorbent, and synchronously detecting hormones such as aldosterone, methoxyadrenaline and cortisol and metabolites thereof by using a high-throughput immunity analyzer; integrating detection data based on a dynamic threshold algorithm to generate a screening report, carrying out weighted scoring on positive samples in combination with clinical features, screening high-risk candidate samples, carrying out 24-hour urine retest on the high-risk samples, and if a retest result is still positive, carrying out referral treatment to a specialized department for disease cause definite diagnosis; according to the method, the technical problems of complex sample treatment, low multi-step efficiency and high false positive rate in the prior art are solved, efficient and accurate screening of endocrine hypertension is realized, and reliable support is provided for early diagnosis and targeted treatment.

Disclosed herein are methods, compositions, and devices for use in early detection of cancer. The claimed methods include the detection of minimal residual disease (MRD) by analysis of urine samples. The method may involve the use of methyl binding domain proteins or by direct sequencing and may involve detection of somatic variants or detection of methylated cytosines. Also claimed is a method which determines a cancer status by generating an input vector based on quantitative measures created from urine sequence reads and analysing the input vector with a machine learning based model.

The invention provides a kit for synchronously detecting catecholamine and metabolites thereof and a detection method, and relates to the technical field of analysis and detection. The kit comprises a diluent, a solid-phase extraction reagent, an LC-MS / MS detection reagent, a solid-phase extraction device and a solid-phase extraction filler. Wherein the solid-phase extraction filler creatively adopts an amphiphilic ion exchange microsphere containing two functional groups, namely carboxyl and quaternary ammonium at the same time. The kit can be used for synchronously and efficiently separating and detecting eight catecholamines and metabolites thereof in a urine sample; and 6 kinds of catecholamines containing amino groups and metabolites thereof in a blood sample can be separated and detected. When the kit is used for separating and detecting catecholamine and metabolites thereof, the extraction and recovery rate is high, the linear range covers the reference value range of normal detection, the detection time of a urine sample can be remarkably shortened, the detection efficiency of mass spectrum is improved, and popularization and application in clinical detection are facilitated.

Device and methods for detection of kidney cancer using urine samples are disclosed herein. The device and methods enable isolation and quantification of kidney tumor cells in urine samples from a subject in need thereof.

In the urine bottom visible component parameter and detection and identification AI training method, a urine sample is preprocessed to obtain a microscopic examination sample; precipitating visible components to the bottom layer; shooting the tiled microscopic examination sample image; identifying and marking the sediment visible components in the urine sediment visible components, obtaining a marked picture, carrying out AI training, and obtaining an AI feature data set A of the urine sediment visible components; the identification and labeling comprise unknown categories under the secondary classification and the primary classification; the unknown categories under the primary classification comprise unknown category casts, unknown category crystals and unknown category cells. Identifying bottom visible components in urine in the selected area S1 of the image by using an AI identification algorithm; the identified bottom visible components comprise unknown categories under the second-level classification and the first-level classification. According to the method, secondary classification recognition is carried out on recognizable visible components sinking to the bottom, primary classification of unknown categories is provided for some visible components which cannot be subjected to secondary classification recognition, the real situation can be reflected more accurately, and the utilization efficiency of subsequent data is improved.

The invention provides a urine HCG full-automatic detection and urine routine combined detection system, which comprises a host shell, an interactive interface, a sample interface, a USB interface, a sampling and sample introduction module, a urine detection module, a urine detection module, a urine detection module and a urine routine detection module, the microfluidic and reagent module is used for automatically distributing and reacting urine samples; the multifunctional automatic detection module is used for performing optical detection and electrochemical detection and completing combined detection of HCG and urine routine; the data processing module is used for collecting original signals and carrying out data processing and calculation to obtain HCG concentration, urine routine multi-parameter results and a detection report; and the intelligent diagnosis support module is used for performing medical correlation analysis and medical record intelligent diagnosis. Through combined detection of HCG and urine routine, HCG and urine routine results can be obtained at the same time, the detection efficiency is improved, and the detection accuracy and sensitivity are enhanced through optical detection and electrochemical detection processes.

A urine collecting and analyzing apparatus for a toilet. the apparatus including a housing with a seat riser that mounts to the toilet rim and a measurement chamber that extends downwardly into the bowl. a urine collecting basin. a flushing system to clean the apparatus. and a controller for data processing and transmission. The basin has a bowl shape to collect urine for testing and is composed of two side panels or two side panels and a front panel. The panels are moved between a storage position along the housing and a collecting position forming the basin by a motorized mechanism. A transfer tube with a flow rate sensor and pump connects the basin to the measurement chamber. The flushing system feeds water through a flushing tube into the basin through an array of nozzles and cleans the entire surface of the basin.