1051 results about "Urine sample" patented technology

The invention relates to a composition for identifying bladder cancer based on urine sample detection and application thereof, and belongs to the technical field of molecular biology. According to the composition disclosed by the invention, the bladder cancer is identified by detecting the expression of one or more of hsa-miR-423-5p, hsa-miR-155-5p and hsa-miR-590-5p in a urine sample; the composition comprises a specific reverse transcription stem-loop primer, a specific detection forward primer, a specific detection probe and a universal reverse primer. According to the composition disclosed by the invention, the expression of hsa-miR-423-5p, hsa-miR-155-5p and hsa-miR-590-5p in a urine sample is detected, and a binomial logistic regression model is combined, so that auxiliary judgment is performed on the bladder cancer, the sensitivity and the specificity are relatively high, and the accuracy of identifying the bladder cancer can be improved. The invention further provides a kit containing the composition, the detection object of the kit is a urine sample, and the kit has the advantages of being small in sample dosage, noninvasive in sampling, convenient, rapid, small in risk and the like.

The present invention provides a method for evaluating urinary system diseases based on urine detection, which belongs to the technical field of urology. The method for evaluating urinary system diseases based on urine detection comprises: adding corresponding reagents to three urine samples in sequence according to a preset time point sequence, and obtaining urine sample images after each addition of the reagents; performing image preprocessing on each acquired urine sample image, extracting image features including color changes, sediment morphology and distribution; establishing a time series feature data set, arranging and combining the image features corresponding to each time point in time sequence; using a pre-trained image recognition model to analyze the image features in the time series feature data set, and obtaining feature vectors; the method can solve the problems of high examination cost, great trauma, long examination time, limited diagnostic accuracy, etc. that the existing means of diagnosing urinary system diseases generally have.

The invention discloses a screening method of metabolic markers for distinguishing non-colorectal cancer diseases and colorectal cancer and application of the screening method. After a urine sample is treated, high-flux gas chromatography-mass spectrometry (GC-MS) derivatization polar metabolite analysis and liquid chromatography-mass spectrometry (LC-MS) polar metabolite detection are respectively carried out. The method comprises the following steps: sequentially carrying out homogenization treatment on obtained metabonomics data, screening 14 metabolic markers from the metabonomics data by adopting a univariate and multivariate statistical analysis method, and selecting 10, 8 and 4 metabolic markers from the 14 metabolic markers, so that the method also has a relatively good distinguishing effect on non-colorectal cancer diseases and colorectal cancer diseases. The invention discloses a non-invasive, high-accuracy and non-invasive urine metabolite marker combination, which improves the diagnosis and evaluation capability of early patients with colorectal cancer so as to realize early discovery, early diagnosis and early intervention clinically, thereby reducing the morbidity and mortality of the patients with colorectal cancer.

The invention discloses a detection device for the endocrinology department, and relates to the technical field of urine sugar detection.The detection device comprises a conveying belt, a first side plate is arranged on one side of the conveying belt, a second side plate is arranged on the other side of the conveying belt, a movable groove body is arranged above the conveying belt, and a plurality of limiting cylinders allowing urine bottles to be inserted therein are arranged in the movable groove body; two clamping blocks are arranged on the inner wall of the limiting cylinder, a plurality of clamping parts used for clamping test paper are arranged above the conveying belt, each clamping part comprises a first clamping handle and a second clamping handle, a first sliding plug is arranged above the first clamping handle and the second clamping handle, a fixing cylinder is arranged on the outer wall of the first sliding plug, a first spring is arranged outside the fixing cylinder, and a second sliding plug is arranged above the second clamping handle. A fixing disc is arranged on the outer wall of the fixing cylinder, a detection unit for pushing the first sliding plug to slide downwards in the fixing cylinder is arranged on one side of the fixing disc, in the device, manual detection of medical staff is not needed, automatic detection of multiple urine samples is achieved, and the risk of infection of the medical staff is reduced.

The invention discloses application of small extracellular endovesicular protein as a prostatic cancer diagnostic marker, and is characterized in that the small extracellular endovesicular protein is plasma sample sEV protein LAMB1 and / or urine sample sEV protein Histone H4, and the plasma sample sEV protein LAMB1 is applied as a metastatic prostatic cancer diagnostic marker. The sEV protein LAMB1 of the plasma sample is combined with a prostate specific antigen to serve as metastatic prostate cancer; the application of the sEV protein Histone H4 in the urine sample as a prostate cancer grading diagnosis marker, the application of the sEV protein Histone H4 in the urine sample as a high-risk prostate cancer diagnosis marker and the application of the combination of the sEV protein Histone H4 in the urine sample and a prostate specific antigen as the high-risk prostate cancer diagnosis marker have the advantages of high sensitivity and strong specificity.

The ureter and urine bag comprises a urine bag body, a spiral pipe, a connecting pipe, a threaded connector pipe and a urine guiding pipe, the threaded connector pipe comprises a pipe body and an insertion pipe, internal threads with the thread pitch being 1.8-2.2 mm are machined on the inner wall of the pipe body, external threads matched with the internal threads are machined on the outer wall of the insertion pipe, and the spiral pipe is connected with the connecting pipe. Two groups of wing plates are symmetrically arranged on the outer wall of the cannula to provide rotating acting points, and a collecting assembly is integrated on the side wall of the urine bag and used for aseptically collecting urine samples. Traditional plug-pull type connection is replaced by a precise thread fit structure, a clear rotating force application point is provided for medical staff, operation is labor-saving and convenient, connector pollution is avoided, the urinary tract infection risk is effectively reduced, and the nursing comfort of a patient is improved.

The invention discloses a nucleic acid aptamer for accurate molecular typing of multi-parameter bladder cancer cells and application of the nucleic acid aptamer, and belongs to the technical field of biology. The technical problem to be solved in the invention is how to realize early accurate diagnosis of bladder cancer and molecular typing of bladder cancer in clinic. In order to solve the technical problem, the invention comprises nucleic acid aptamers including nucleic acid aptamers Seq12a, BCfsw1-3a, BCfsw1-4a, BCfsw1-10a, BCfsw2-19a and BCfsw2-19a, and a composition composed of the nucleic acid aptamers. According to the application, nucleic acid aptamer screening is carried out aiming at different levels of bladder cancer cells and bladder cancer clinical urine samples to obtain nucleic acid aptamer groups covering information of different types of bladder cancer, and cell molecular typing is realized on bladder cancer cells and normal cell lines; and the kit is preliminarily applied to clinical urine sample molecular typing and bladder cancer recurrence course monitoring.

The invention discloses a method for detecting the contents of VMA, HVA and 5-HIAA in urine by using liquid chromatography-tandem mass spectrometry. The method comprises the following steps: S1, removing VMA, HVA and 5-HIAA in urine to obtain a blank urine matrix; s2, weighing VMA, HVA and 5-HIAA solid powder, fixing the volume with methanol, mixing the dissolved liquids, diluting with methanol-water to obtain a VMA, HVA and 5-HIAA working solution, and finally diluting step by step with a blank urine matrix to obtain a calibration product; s3, respectively taking a urine sample to be detected, the calibration substance and the quality control substance, adding the mixed internal standard working solution of VMA, HVA and 5-HIAA isotopes, uniformly mixing, centrifuging, and taking supernate; s4, separating the supernate by using liquid chromatography; s5, the separated supernate enters a mass spectrum to be detected, and the content of VMA, HVA and 5-HIAA in the urine sample to be detected is obtained. The detection method disclosed by the invention is simple and rapid in pretreatment, high in specificity, low in reagent consumption cost, free of environmental pollution, small in required sample size, easy in high-throughput operation and suitable for being developed into a kit for conventional clinical detection.

The invention discloses a method for rapidly detecting common candida of urinary tract infection based on a nucleic acid mass spectrometry technology. The method comprises the following steps: extracting total nucleic acid from a urine sample of a patient; amplifying the extracted total nucleic acid by adopting a PCR (Polymerase Chain Reaction) technology to obtain an amplification product containing a target sequence; analyzing a characteristic peak map of the amplification product containing the target sequence by using a single-base extension primer and a matrix-assisted laser desorption / ionization time flight mass spectrometry technology; and determining whether specific candida exists in the sample according to the characteristic peak map. According to the invention, a set of detection system suitable for common candida is obtained through sequence screening and optimization of the amplification primers and the single-base extension primers, five common candida of UTI are detected based on the system, and a nucleic acid mass spectrometry technology is combined, so that the result can be clearly distinguished, a complex traditional detection means is avoided, and the detection efficiency is improved.

The invention provides a diabetic nephropathy early warning method, system, equipment and medium, and relates to the technical field of medical data analysis and prediction.The method comprises the steps that multiple urine samples of a user are collected, target urine protein detection is conducted, and multiple urine protein concentrations are obtained; each urine protein concentration is combined with a normal urine protein concentration set to be input into an abnormal data recognition tree, the number of layers of multiple recognition nodes is obtained, and the detection confidence coefficient is obtained through calculation; configuring an abnormal proportion according to the detection confidence coefficient, inputting a plurality of urine protein concentration combination normal urine protein concentration sets into an abnormal data identification tree, deleting urine protein concentrations divided into abnormal data in the abnormal proportion before, and obtaining screened urine protein concentrations; according to the screened urine protein concentration, an auxiliary early warning coefficient is obtained through calculation, and early warning is carried out. The technical problem of low early warning accuracy of diabetic nephropathy in the prior art is solved.

Disclosed here are kits comprising pre-packed stabilizing solutions for stabilizing combinations of biomarkers demonstrating sufficient accuracy and specificity for identifying kidney injuries. Such kits can be better adapted for sample collection at a subject's dwelling, thus easing the burdensome requirement of continuous monitoring for kidney injury.

The invention discloses a monitoring method and system for prevention and treatment of renal fibrosis. The monitoring method comprises the following steps: collecting biomarker data in a urine sample of a patient in real time through a biosensing detector; transmitting the acquired biomarker data to a data processing center, and establishing a renal fibrosis risk assessment model of the patient; according to the risk assessment result, making a personalized prevention and treatment scheme, and monitoring the execution condition of the prevention and treatment scheme in real time; periodically detecting blood sample indexes of a patient, and combining with the biomarker data to obtain a comprehensive evaluation effect of renal fibrosis prevention and treatment; and feeding back the comprehensive evaluation result to a patient and medical staff, and adjusting the personalized prevention and treatment scheme according to the comprehensive evaluation result. According to the scheme, accurate and dynamic monitoring on prevention and treatment of the renal fibrosis can be achieved, the prevention and treatment effect of the renal fibrosis can be improved, and the life quality and prognosis of a patient are improved.

The invention discloses a urine sampling device for the endocrinology department and a sampling method, and belongs to the technical field of medical analysis. Comprising the following modules: an automatic sampling control module for realizing automatic control of the whole urine sampling process, ensuring that sampling accords with a standard process, and supporting automatic sub-packaging and storage management of samples; the sample quality control module is used for monitoring the physical characteristics of the samples in real time, automatically evaluating the effectiveness of the samples in combination with environmental parameter monitoring, and identifying and marking abnormal samples; the data acquisition and processing module is used for comprehensively acquiring physiological data, nutrition metabolism data, operation process data and sample tracing information, and performing cleaning, standardization and fusion processing to generate a standardized data set; the intelligent diagnosis and analysis module is used for intelligently detecting abnormal physiological indexes and carrying out disease risk prediction and early warning; and the man-machine interaction module provides a visual operation interface and integrates multi-level user authority management, automatic report generation and remote monitoring.

Doubly labeled water with enhanced protocol. One embodiment is a method including providing, to a user, a doubly labeled water (DLW) dose for the user to ingest, the DLW dose including deuterium and oxygen-18, wherein an amount of the deuterium is less than 0.12 grams per kilogram (g / kg) of body water of the user, and wherein an amount of the oxygen-18 is less than 0.18 g / kg of body water of the user. The method also includes receiving, from the user, a non-cooled shipment of urine samples collected in connection with ingestion of the DLW dose, wherein the urine samples remain uncooled after collection and during transit for a period of up to 24 days. The method further includes processing the urine samples with a liquid water isotope analyzer to determine one or more metabolic parameters of the user.

The invention relates to methods, devices, and software for distinguishing hemoglobin (Hb) from myoglobin (Mb) in urine samples using peroxidase-reactive test strips. A time-resolved video of the color change on the test pad is recorded, and hue values are extracted and analyzed over time. Diagnostic differentiation between Hb and Mb is achieved by evaluating the hue-time curve, in particular via endpoint hue values and the ratio of integrated hue values in distinct time intervals. The invention further comprises a standardized optical setup for recording, a software-based evaluation algorithm, and a refillable cartridge for in-situ urine analysis devices containing appropriate test fields. The invention enables combined testing with additional urine parameters and is suitable for medical and non-clinical applications such as sport and home diagnostics.

The invention relates to the field of environmental chemistry and biological sample analysis, in particular to an analysis method for detecting oxidative stress biomarkers in urine. The analysis method capable of simultaneously detecting dityrosine, 8-hydroxyguanosine, 8-hydroxydeoxyguanosine and 4-hydroxynonenal mercaptouric acid in human urine is provided on the basis of liquid chromatography-tandem mass spectrometry, effective purification of a urine sample is achieved through specific pretreatment, the matrix effect is reduced, and the detection sensitivity is improved. And specific liquid chromatogram parameter conditions are combined, so that simultaneous detection of the four oxidative stress biomarkers is realized. The method is good in selectivity, high in sensitivity and high in detection speed, the problem that polar and non-polar components cannot be measured at the same time at present is solved, the contained oxidative stress biomarkers are more comprehensive and more reasonable, and the oxidative stress damage condition of the organism can be better reflected.

There is a provided a urine collection device for collecting an uncontaminated urine sample from a pre-continent or an incontinent user, the urine collection device comprising: a receptacle configured to be placed within a nappy or an incontinence pad of the user to receive and retain a urine sample collected from the user in use, wherein the receptacle comprises: a receiving portion for receiving the urine sample, the receiving portion comprising an opening configured to engage with a human body portion surrounding external genitalia of the user, thereby enabling urine exiting the body of the user to be captured directly in use; a reservoir for retaining the received urine sample in use; and an outlet for draining the retained urine sample in use, wherein the opening is larger than the outlet.

An intermittent catheter package including a plurality of components and a catheterization tray configured to facilitate a catheterization procedure. The plurality of components include a urinary catheter fluidly connected to a urine-drainage bag and a sampling-port access device configured to fluidly connect to a urine-sampling port of the urinary catheter or the urine-drainage bag for aseptic collection of one or more urine samples.The catheterization tray includes at least a first compartment, a second compartment, a third compartment, and a fourth compartment. The second compartment is connected to the third compartment Step-by-step surface instructions for performing the catheterization procedure are incorporated into the catheterization tray.

The utility model relates to the field of treatment equipment, in particular to blood and urine sample pretreatment equipment. The technical problems that blood and urine sample pretreatment is divided into a step-by-step process flow, a plurality of sample tubes are sequentially treated and operated, professional personnel need to carefully observe during each operation, the condition of sample pollution is easy to occur, and the overall treatment effect is reduced are solved. According to the technical scheme, the blood and urine sample pretreatment equipment comprises a vortex uniform mixing instrument, a climbing adjusting module, a filling treatment assembly and a balance input assembly; the reagent solution is put into the vortex mixing instrument through lifting operation, taking and putting operation is carried out, manual touch is not needed, the filling treatment assembly carries out sliding and climbing adjustment operation along the climbing adjustment module, a corresponding reagent solution is sucked in through a dripping pipe, a centrifugal pipe is directly filled with the reagent solution, and meanwhile extraction operation is carried out after centrifugation. An operator is prevented from touching the centrifugal tank, and the sample is prevented from being polluted.

The invention relates to the field of medical treatment, in particular to application, a model and a method of a protein fragment of urine regulatory protein in preparation of a kidney disease early detection product, and the amino acid sequence of the protein fragment of the urine regulatory protein is any shown in SEQ ID NO.1-8. The model constructed according to the protein fragment of the urine regulatory protein can be used as a non-invasive tool for early detection and monitoring of kidney diseases, and by analyzing the unique mode of the protein fragment existing in a urine sample, the model which indicates renal insufficiency, renal function and renal function can be identified before the traditional clinical biomarker is abnormal. Therefore, intervention can be carried out earlier.

The present invention provides a kit and a detection method for synchronously detecting catecholamines and their metabolites, which relates to the technical field of analytical detection. The kit contains a diluent, a solid-phase extraction reagent, an LC-MS / MS detection reagent, a solid-phase extraction device, and a solid-phase extraction packing material. Among them, the solid-phase extraction packing material creatively adopts zwitterionic exchange microspheres containing both carboxyl and quaternary ammonium groups. The kit can not only synchronously and efficiently separate and detect 8 kinds of catecholamines and their metabolites in urine samples, but also separate and detect 6 kinds of amino-containing catecholamines and their metabolites in blood samples. When the above kit is used to separate and detect catecholamines and their metabolites, the extraction recovery rate is relatively high, and the linear range covers the reference value range of normal detection, which can significantly shorten the detection time of urine samples, improve the detection efficiency of mass spectrometry, and is conducive to popularization and application in clinical detection.

The invention discloses a urine sampling device, and belongs to the field of medical examination appliances, the urine sampling device comprises a sampling driver and a disposable sampling assembly mounted on the sampling driver, the sampling driver comprises a case and an adjustable constant-speed moving mechanism, the disposable sampling assembly comprises a disposable urine storage device and a urine receiving device, and the adjustable constant-speed moving mechanism is arranged on the case. A tube head fixing frame is arranged on the adjustable constant-speed moving mechanism, a transverse sliding hole is formed in the side wall of the machine box, the tube head fixing frame penetrates through the transverse sliding hole in a sliding mode and is arranged outside the machine box, an installation clamping frame is arranged on the side wall of the machine box, and the disposable urine storage device is arranged in the installation clamping frame in a clamping mode. The pipe head fixing frame is arranged above the disposable urine storage device in a sliding mode, the bottom end of the urine receiving device is connected with a corrugated pipe, and a liquid discharging pipe head is arranged at the end, away from the urine receiving device, of the corrugated pipe and installed on the pipe head fixing frame. According to the urine sampling device provided by the invention, midstream urine can be accurately sampled according to the urine volume of a patient, and enough sampling amount is ensured.

A disposable diaper product useful for collecting a urine sample from an infant is disclosed. In one example the product may have a liquid control structure overlying a liquid impermeable backsheet, with a plan surface area defining a volume coextensive therewith, wherein a portion of the volume defined by at least 50 percent of the plan surface area contains no more than 50 percent by weight absorbent material. In another example the product may have a liquid control structure overlying a liquid impermeable backsheet, and have an average Liquid Release Ratio of at least 3 percent. A method for collecting a urine sample from an infant is also disclosed, in which a diaper is used for collection.

1. Name of the design product: Women's urine sampling container. 2. Use of the design product: For women's urine sampling. 3. Design key points of the design product: Lies in the shape. 4. Picture or photo that best shows the design key points: Perspective view.

The invention discloses a urine collection and storage device, and relates to the technical field of urine collection devices, the urine collection and storage device comprises a base frame for receiving urine and a liquid guide channel fixedly arranged on the base frame, the base frame is provided with a floating collection mechanism for quantitatively collecting urine at the middle end, and the floating collection mechanism comprises a horizontal base fixedly connected to the inner wall of the base frame; the horizontal base is provided with a horizontal assembly for adjusting the relative position of the transverse base and the liquid guide channel, the two horizontal sides of the middle clamping base are provided with a main inner cavity column and a slave inner cavity column respectively, and the partition frame is provided with a blocking assembly for partitioning and sealing the internal space of the main inner cavity column and the slave inner cavity column. By arranging the floating collection mechanism, midstream urine of a patient can be accurately collected, and meanwhile, tissues and impurities deposited at the bottom of a urine bag are abandoned, so that the purity and representativeness of a urine sample are effectively improved, the accuracy of a detection result is ensured, and the misdiagnosis risk caused by sample pollution or impurity mixing is reduced.

The invention discloses a culture method of high-activity human urine-derived stem cells, which relates to the technical field of biological medicine, and is technically characterized by comprising the following steps: collecting urine samples of pregnant and lying-in women, coating a culture plate with gelatin, inducing adherence, performing passage amplification, performing immunofluorescence identification, performing function detection and other optimized conditions, and separating and culturing the stem cells. Compared with urine-derived stem cells of normal adults, the stem cells derived from pregnant women have stronger activity, higher dryness and faster multiplication capacity. The invention provides a novel and superior cell source for tissue engineering and regenerative medicine.

The present invention belongs to the technical field of urine detection sampling equipment, and discloses a urology urine detection sampling equipment, including a support bin, and also including a rear-segment sampling structure, which is arranged in the inner cavity of the support bin and is used to sample the rear-segment urine, a quantity control structure is arranged in the inner cavity of the support bin and is used to control the sampling amount, a discharge structure is arranged in the inner cavity of the support bin and is used to supplement the sample, and a closing structure is arranged to be slidably connected above the support bin and is used to close the support bin, wherein the rear-segment sampling structure includes a front-segment collecting bin, and the front-segment collecting bin is fixedly connected to the inner cavity of the support bin. The present invention collects the front-segment urine by the front-segment collecting bin, and collects and samples the rear-segment urine by the rear-segment sampling bin, thereby avoiding the bacteria at the user's urethral opening from mixing in the front-segment urine, causing the entire urine sample to be contaminated, affecting the accuracy of the detection, and at the same time, the user does not need to change the urination site, thereby improving the convenience of collection.

The invention discloses a urine sampling device for a clinical laboratory, relates to the technical field of urine sampling and detection, and aims to solve the technical problem that middle-section urine samples are difficult to collect due to physiological degeneration of old people, and the urine sampling device comprises a receiving cup, a collecting and filtering mechanism, a cutting assembly, an extrusion unit and a backflow unit. In the aspect of urine filtration, the collecting and filtering mechanism works cooperatively by means of the bearing cylinder, the circulating cylinder and other components, particles in urine are filtered accurately, and the particles are prevented from interfering with medical urine detection; the front section of urine is accurately intercepted and impurities are removed through the intercepting assembly; when the urine volume is insufficient, the extrusion unit and the backflow unit are tacitly matched to automatically supplement the urine sample volume, in addition, the urine flowing form can be optimized by arranging a cone in the circulation channel, all the assemblies are easy, convenient and flexible to operate, the overall structure is stable and good in sealing performance, and various medical urine detection requirements can be met; a high-quality urine sample is provided, and clinical examination work is powerfully supported.

The invention discloses a metabolite detection method for diagnosing lithangiuria, and relates to the technical field of metabolite detection. The method comprises the following steps: collecting and preprocessing a urine sample, and generating a composite fluorescence signal based on the preprocessed urine sample so as to obtain a composite function; constructing an orbital function system, and performing projection processing on a composite function to obtain initial orbital energy; based on the initial orbital energy, a mutual exclusion function between metabolites is introduced, an effective energy contribution value is calculated, and a decoupled energy response amount is obtained; converting the decoupled energy response quantity into a concentration estimation value, and generating a rotation response value based on the concentration estimation value; generating a coupling threshold based on the concentration estimation value and the rotation response value; and carrying out risk scoring based on the coupling threshold value to obtain an individual risk score value of the urinary calculi. The invention solves the problems of inaccurate concentration estimation of the metabolite and low metabolite detection precision caused by inaccurate analysis of the metabolite under complex interference conditions in the existing metabolite detection method.

A method for characterizing disease activity in a systemic lupus erythematosus patient (SLE), comprising obtaining a dataset associated with a blood, serum, plasma or urine sample from the patient, assessing the dataset for a presence or an amount of protein expression of at least one innate serum or plasma mediator, assessing the dataset for a presence or an amount of protein expression of at least one adaptive serum or plasma mediator biomarker, assessing the dataset for a presence or an amount of at least one chemokine / adhesion molecule biomarker, assessing the dataset for a presence or an amount of at least one soluble TNF superfamily biomarker, assessing the dataset for a presence or an amount of at least one inflammatory mediator biomarker, assessing the dataset for a presence or an amount at least one SLE-associated autoantibody specificity biomarker and calculating a Lupus Disease Activity Immune Index (LDAII) score.