The invention relates to a fast drug sensitivity detection kit for vibrio, which can be applied to an aquiculture site. According to the invention, the detection kit comprises a detection plate, a thallus collection tool, bacterium enrichment liquid, a drug sensitivity detection culture medium, a turbidimetry tube, an inoculation burette and a colorimetric card, wherein the detection plate comprises inspection holes enveloping drugs and color indicators for drug sensitivity detection, and the color indicators are Alamar Blue. According to the invention, fish peptone, Ca2+ and Mg2+ are added based on the MH culture medium and the concentration of NaCl is adjusted, and the culture medium is used as the bacterium enrichment liquid of vibrio, which can effectively promote the growth of the vibrio and enables the bacterium enrichment liquid to reach the required concentration in a short time, so as to shorten the time of drug sensitivity detection. At the same time, a blue dye, namely the Alamar Blue, which is safe and nontoxic to the cells is used; the Alamar Blue has no toxic effect on cells, and can be enveloped in the micropores of the drug sensitivity detection plate directly without influencing the growth of the bacteria, so that the detection operation is simpler and more convenient and quick; and the proliferation dynamic of the bacteria can be continuously monitored.