Urinary transferrin immuno-turbidimetry reagent and preparation method thereof

Abstract

The invention discloses a urinary tranferrin immuno-turbidimetry kit. The kit comprises a freeze-dried R1 reagent and a freeze-dried R2 reagent, which are fixed in a same container. The freeze-dried R1 reagent and freeze-dried R2 reagent are prepared by adding an excipient and a protective agent into a liquid R1 reagent and a liquid R2 reagent, and then freeze-drying the reagents. The liquid R1 reagent is a buffer solution containing PEG. The liquid R2 reagent is anti-human transferrin polyclonal or matched monoclonal antibody couple latex, or anti-human transferrin polyclonal antibody or matched monoclonal antibody coupled colloidal gold. The detection operation is convenient, the time is saved, at the same time, the transportation and preservation are convenient, the reagent can be transported in cold areas, and the reagent stability is improved.

Description

technical field [0001] The invention relates to the field of immunoturbidimetry, and specifically relates to a protein detection kit and a preparation process thereof. Background technique [0002] Transferrin (Transferrin, Tf) is a metal-binding glycoprotein synthesized by the liver. It is classified as β1-globulin by electrophoresis. Its main physiological function is to transport iron, and it belongs to the acute phase reflection protein. Under normal circumstances, Tf cannot pass through the glomerular filtration membrane. In early renal injury, the content of heparan sulfate glycoprotein in the inner and outer loose layers of the glomerular basement membrane is reduced, which significantly reduces the negative charge sites and makes the negatively charged cells (PI=5.7) The circulating molecular protein Tf is filtered from the glomerular filtration membrane and appears in the urine. Clinical significance of urine Tf detection: It is one of the indicators of early glome...

AI Technical Summary

Problems solved by technology

[0004] At present, most of the reagent kits used for turbidimetric detection in the domestic and foreign markets are liquid double reagents; since freezing and thawing will affect the performance of R2 in the reagents and inactivate the product, there are no freeze-dried reagents. Therefore, there are big problems in the transportation or storage of reagents in northern regions in winter; at the same time, more and more professional departments of hospitals or primary hospitals hope that manufacturers can provide POCT reagents and instruments, so that detection data can be obtained as soon as possible, and patients can be diagnosed and treated in time. Among them, the ready-to-use POCT reagents are especially favored by doctors, but the ready-to-use immunoturbidimetric reagents occupy a large space, and the laboratory cannot provide enough refrigerated space to store the reagents. Transportation and storage of turbid reagents

[0005] At the same time, due to the mutual interference between reagents, double reagents have been used clinically, and the problem of single reagent has not been solved; single reagent will make the operation steps simpler and reduce time

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