48 results about "Transferrin" patented technology

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

This invention discloses a NK cell culture kit, its culture method, and its application. The kit includes an amplification medium, a high-efficiency induction medium, an NK-A coating solution, an NK-B mixture, and an NK-C mixture. The amplification medium contains basal medium, NAD+, human serum albumin, transferrin, β-glucan, glutathione, β-mercaptoethanol, and linoleic acid. The high-efficiency induction medium, in addition to the amplification medium components, contains soybean peptides, nicotinamide, inulin, and N-acetyl-L-cysteine. The NK-A coating solution contains heparin sodium, monoclonal antibodies, and antibodies. The NK-B mixture contains Inbakicept, IL-2, and IL-15. The NK-C mixture contains linolenic acid, IL-2, and IL-18. The Inbakicept factor in the kit can activate NK cells and enhance their cytotoxicity; linolenic acid can inhibit T cell proliferation, increase NK cell purity, and enhance cell efficacy.

The invention belongs to the technical field of molecular biology, and relates to an application of transferrin (TF) as an internal reference protein in preparation of a reagent for detecting exosome proteins of neurodegenerative diseases. The TF can maintain a stable expression level in nerve cells, brain tissues, blood plasma and serum-derived exosomes of a subject suffering from neurodegenerative diseases, including Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease, and also including neurodegenerative diseases such as Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease. The TF expression variation coefficient is obviously lower than that of a common exosome marker, and the expression level of TF has stronger correlation with the total protein amount of the exosome and is not influenced by external factors such as cell inflammation stress. The TF as the internal reference protein has more excellent performance, can help to more accurately reflect the total loading amount of the sample, and provides a more stable and reliable standardized tool for the field of exosome research.

The application discloses a kind of R8 and Tf co-modified mebendazole liposome targeted preparation and its preparation method, application, belong to the field of biological medicine. In view of the poor solubility of mebendazole in water, difficult to penetrate blood-brain barrier, the problem of insufficient tumor targeting in brain glioma treatment, the application adopts film hydration-step modification process, and octaarginine (R8) and targeting ligand transferrin (Tf) are simultaneously modified on the surface of mebendazole-loaded liposome, and the preparation prepared by the application has an encapsulation efficiency of not less than 95%. The preparation can break through blood-brain barrier and precisely enrich in brain glioma lesions, and the in-vivo tumor inhibition rate is more than 90%, with low toxicity and side effects. The preparation can be used for preparing brain glioma therapeutic drugs, and provides a new drug for targeted treatment of brain glioma.

The invention provides an induced pluripotent stem cell culture medium and a culture method of induced pluripotent stem cells. The induced pluripotent stem cell culture medium comprises a basic culture medium, a composite additive A and a composite additive B, the composite additive A comprises 5-30 [mu] g / mL of recombinant human insulin growth factors, 1-5 ng / mL of recombinant human transforming growth factors, 50-200 ng / mL of recombinant human basic fibroblast growth factors, 7-20 [mu] g / mL of sodium selenite, 0.5-2 mg / mL of albumin, 50-70 [mu] g / mL of ascorbic acid and 5-20 [mu] g / mL of transferrin; the composite additive B is prepared from 50 to 300mg / mL of a Stock B solution, 0.5 to 5 percent (v / v) of a non-essential amino acid solution, 50 to 200ng / mL of DL-piperidinecarboxylic acid, 50 to 200mu g / mL of gamma-aminobutyric acid, 0.1 to 1 percent (v / v) of a lipid concentrated solution, 0.5 to 5 percent (v / v) of an L-alanyl-L-glutamine solution and 0.1 to 0.1 percent of mercaptoethanol. The culture medium for the induced pluripotent stem cells provided by the invention can ensure the self-renewal capability of the iPSCs in a long-term passage process; and the genetic stability of the iPSCs cells is optimized according to the genome integrity and pluripotent state.

Provided are transferrin receptor binding VHH domains as well as delivery complexes and methods for targeting cells of interest for delivery of an active cargo such as an oligomeric agent. In particular, the delivery complex may comprise modified Fc domain and a transferrin receptor binding VHH domain.

The invention belongs to the technical field of biology, and discloses a serum-free culture medium and application thereof in obtaining nerve cells through embryo fibroblast transdifferentiation. The serum-free culture medium consists of a basic culture medium, a protein additive and cell culture auxiliary components, the protein additive consists of transferrin, a leukemia inhibition factor and a basic fibroblast growth factor; the final concentration of the transferrin is 5 ng / mL-30 ng / mL, the final concentration of the leukemia inhibition factor is 50 ng / mL-150 ng / mL, and the final concentration of the basic fibroblast growth factor is 10 ng / mL-25 ng / mL. The serum-free culture medium can promote the conversion speed of transdifferentiation of fibroblasts into nerve cells, can significantly improve the transdifferentiation efficiency, and can improve the cell survival rate of transdifferentiation of mouse embryo fibroblasts into nerve cells.

The invention provides a biotinylated Angiopep-2 modified targeting nano material as well as a preparation method and application of the biotinylated Angiopep-2 modified targeting nano material. According to the preparation method of the biotinylated Angiopep-2 modified targeting nano-material, Angiopep-2 is directionally anchored through a high-affinity biotin-streptavidin system, ligand conformation inactivation caused by chemical coupling is avoided, targeting activity is maintained, the used Angiopep-2 can help nanoparticles to be specifically recognized and combined and mediate the nanoparticles to cross a blood brain barrier to enter the brain, and the targeting nano-material can be used for preparing the targeted nano-material. The drug concentration in brain tissues is increased, so that the cure rate of brain diseases is improved, the brain targeting efficiency is far higher than that of transferrin and lactoferrin, and the application value is great; by entrapping curcumin and quercetin, a hydrophilic shell is provided, so that the water solubility of curcumin and quercetin is improved, and the bioavailability of curcumin and quercetin is improved.

The invention belongs to the technical field of cell therapy, and relates to a serum-free and heterologous component-free NK cell culture medium as well as a preparation method and application thereof. The culture medium comprises a classical culture medium, a proliferation promoting component, vitamins, trace elements, lipids, transferrin, an energy substance and beta-mercaptoethanol, the proliferation promoting component is prepared from human albumin, resveratrol, insulin and hydroxyproline. On the basis of a serum-free and heterologous component-free NK cell culture medium, IL-12, rapamycin, polyinosinic-polycytidylic acid and human AB plasma are additionally added to obtain an activated culture medium, the activated culture medium and an amplification culture medium are jointly used, the culture effect is better, and amplification components IL-2 and human AB plasma are additionally added to obtain an amplification culture medium. Through precise component design, the NK cell in-vitro proliferation efficiency, motility, purity and killing ability are remarkably improved, and key problems existing in a traditional culture medium are efficiently solved.

A hematopoietic stem cell (HSC) proliferation medium includes a basal medium, 0.1 ng / mL to 200.0 ng / mL of thrombopoietin (TPO), 0.1 ng / mL to 200.0 ng / mL of stem cell factor (SCF), 0.1 ng / mL to 20.0 ng / mL of interleukin-3 (IL-3), 0.1 ng / mL to 100.0 ng / mL of interleukin-6 (IL-6), 0.1 ng / mL to 200.0 ng / mL of Flt-3 ligand (FL), 0.1 μg / mL to 20.0 μg / mL of insulin, 0.1 μg / mL to 120.0 μg / mL of transferrin, 0.1 g / L to 3.0 g / L of human serum albumin (HSA), 0.1 μM to 4.0 μM of pyrimido-indole derivative UM729, and 0.1 μM to 2.0 μM of aryl hydrocarbon receptor (AhR) antagonist StemRegenin 1 (SR1). Methods for in vitro proliferation of hematopoietic stem cells (HSCs) and in vitro production of hematopoietic progenitor cells (HPCs) using the HSC proliferation medium are also provided.

Disclosed are novel vaccine compositions comprising modulated Transferrin Binding Protein B (TbpB) polypeptides or immunogenic portions thereof. Notably, the TbpB polypeptides are modulated with respect to domains known as loop domains located in the C-terminal lobe of TbpB polypeptides. The vaccine compositions may be used to ameliorate, treat or prevent pathogenic infections in humans caused by pathogenic H. influenzae strains. Related methods and uses are also disclosed.

The invention discloses a skincare stem cell exosome separation and extraction method, and relates to the technical field of biological medicine, the skincare stem cell exosome separation and extraction method comprises the following steps: S1, culture of mixed stem cells: unfreezing urine-derived stem cells and pilose antler stem cells, resuspending in an improved DMEM culture medium, and carrying out mixed culture to prepare a mixed culture solution; epidermal growth factors, cholera toxin, penicillin, streptomycin, transferrin, IL-2 and IL-15 are added into the improved DMEM culture medium; s2, pretreatment of the mixed culture solution: performing centrifugal treatment on the mixed culture solution, and separating to obtain a precipitate and a supernatant, the precipitate being mixed stem cells. The urine-derived stem cells and the pilose antler stem cells are subjected to mixed culture and matched with the improved culture medium containing IL-2, IL-15 and other components, so that the yield and biological activity of the exosome are remarkably improved, high purity and low impurity protein residue of the exosome are achieved by means of a complete purification process of multi-step centrifugation, ultrafiltration concentration and filter membrane filtration, and the yield of the exosome is improved. The skin allergy stimulation risk is effectively reduced.

The invention discloses a preparation method and application of autonomic ganglion organoid, and relates to the technical field of biology. The method comprises the following steps: 1) providing neural crest stem cells; 2) differentiating the neural crest stem cells into autonomic ganglion-like organs through the combined action of added external factors and internal factors; wherein the external factors comprise a bFGF (basic fibroblast growth factor), an IGF-1 (insulin-like growth factor), a BDNF (brain-derived neurotrophic factor), a GDNF (brain-derived neurotrophic factor), an NGF (nerve growth factor), an NT-3, an EGF (epidermal growth factor), Forskolin, a DAPT (diaminopropyltriethoxysilane), an insulin, a Transferrin, an Activin A and a BMP4 (bone morphogenetic protein 4); the addition of the intrinsic factors is that the following four transcription factors, namely Ascl1, Hand2, Phox2a and Phox2b, are overexpressed in the neural crest stem cells at the same time. The organoid prepared by the scheme contains various neurons similar to the autonomic ganglion in vivo, Schwann glial cells and satellite glial cells. The prepared autonomic ganglion organoid can be applied to research on development regulation of autonomic ganglions and occurrence and development mechanisms of various human diseases, development of therapeutic drugs and the like.

The invention provides a serum-free medium for maintaining dryness of mesenchymal stem cells as well as a preparation method and application of the serum-free medium, and belongs to the technical field of biological medicines. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, the basic culture medium is a DMEM / F12 culture medium, and the exogenous additive components comprise bFGF, EGF, PDGF-BB, recombinant human fibronectin, laminin, insulin, transferrin, sodium selenate, beta-mercaptoethanol and ascorbic acid. The serum-free culture medium provided by the invention is a special culture medium for the mesenchymal stem cells, which is completely free of animal-derived components, is suitable for large-scale amplification of clinical-grade cell treatment products, is relatively low in preparation cost and high in stem differentiation capability, and can remarkably improve the proliferation efficiency of the cultured mesenchymal stem cells.

The invention belongs to the technical field of cell culture, and particularly relates to a method for inducing differentiation of human pluripotent stem cells into myocardial cells, glycidyl methacrylate is found and applied as a key induction factor for the first time, and the optimal concentration of glycidyl methacrylate in a differential culture medium is 0.5-5.1% by volume. According to the method, human pluripotent stem cells can be efficiently induced into cardiac muscle cells with an autonomous pulsation function within 7-10 days by utilizing a chemical component determination system containing specific concentration of transferrin, L-ascorbic acid and other additives through a definite induced differentiation stage and a culture maintenance stage, and the proportion of cTnT positive cells is stably higher than 80% within 9 days. According to the scheme, the components are clear, the batch stability is good, the differentiation efficiency is remarkably superior to that of a traditional method, and a high-quality cell source is provided for heart disease research, drug screening and cell therapy.

This invention belongs to the field of stem cell culture medium technology, and provides a cell culture medium for enriching extracellular vesicles derived from stem cells and its preparation method. This invention disperses sodium hyaluronate or sodium hyaluronate-chondroitin sulfate sodium on a defined basic culture medium and grafts porous calcium alginate-gelatin microcarriers onto its surface, while limiting the content of recombinant human insulin, recombinant human transferrin, sodium selenite, and recombinant human serum albumin. This forms a culture system that combines a cell interaction interface with separable microcarriers, improving the enrichment stability of extracellular vesicles derived from stem cells, reducing the risk of solid-liquid separation residues and coupling agent residues, and solving the problem of balancing enrichment capacity with solid-liquid separation and grafting stability with biosafety. It is suitable for in vitro stem cell culture and extracellular vesicle preparation.

The application discloses a kind of serum-free epidermal melanocyte culture medium and its application, method includes by dissolving adenine, hepatocyte growth factor (HGF), glutamine, retinoic acid, vitamin E, nicotinic acid, sodium selenite, sodium hyaluronate and recombination human type XVII collagen are configured into initial culture medium;Preparation of melanocyte stimulating hormone, endothelin-3, transferrin, triiodothyronine, cholesterol, neuroregulin 1 (NRG1), 3-isobutyl-1-methyl purine (IBMX), dibutyryl cyclic adenosine monophosphate (db-cAMP), cholera toxin, hydrocortisone and recombination human insulin mother liquor;Step three: according to culture and concentration, a certain amount of mother liquor of step two is added to initial culture medium.The culture medium can effectively promote human epidermal melanocyte proliferation, melanin accumulation and help to promote human umbilical cord mesenchymal stem cells into human epidermal melanocyte, provide key advantage for obtaining functional melanocyte with high activity and therapeutic potential.

The present disclosure relates to the use of ferritin in lieu of transferrin in culture medium and other compositions or products, such as biomass compositions or cultivated meat compositions. Such compositions or products are suitable for human and / or pet consumption. The invented culture medium is not only able to support long-term growth and culture of animal cells ex vivo but, unlike transferrin-containing culture media, is affordable for both pharmaceutical and food production. The present disclosure also encompasses methods and processes in making and / or manufacturing such cell culture media and / or compositions, as well as their use in products for human and / or pet consumption. The products include for example, food products, feed products, nutraceutical products, and pharmaceutical products.

Disclosed are novel vaccine compositions comprising modulated Transferrin Binding Protein B (TbpB) polypeptides or immunogenic portions thereof. The vaccine compositions may be used to prevent, treat, or ameliorate pathogenic infections in humans caused by pathogenic H. influenzae strains. Related methods and uses are also disclosed.

Disclosed herein are compositions comprising a population of silk fibroin nanoparticles having a polydispersity index (PDI) of 0.5 or less, including but not limited to, a PDI of 0.450 or less, 0.40 or less, 0.35 or less, 0.325 or less, 0.30 or less, 0.275 or less, 0.250 or less, 0.225 or less, or 0.200 or less, wherein a glucagon-like peptide-1 (GLP-1) compound or other protein drug or peptide drug including but not limited to insulin, monoclonal antibodies, and related structures is embedded within the population of silk fibroin nanoparticles, wherein the population of silk fibroin nanoparticles is optionally surface treated with at least one of polyethylene glycol, human transferrin protein, the Fc receptor of IgG, or other novel surface modifiers including but not limited to peptides, proteins, or nanobodies. Methods of making and administering the compositions are described herein.

This disclosure belongs to the field of cell culture technology, and specifically relates to a serum-free culture medium for MSCs and its preparation method. The culture medium contains a fixed ratio of basic nutrients and six key regulatory factors, namely transferrin, ascorbic acid, human serum albumin, human fibroblast growth factor rhFGF, platelet-derived growth factor PDGF-BB, and transforming growth factor TGF-β1. By optimizing the combination and concentration of these six key regulatory factors, the proliferation rate and activity of MSCs are significantly improved. It also provides four suitable formulations: high proliferation, high activity, balanced, and economical. The culture effect is stable and reproducible, with high safety and a simple process, making it suitable for laboratory research and large-scale clinical-grade MSC culture.

The invention relates to the technical field of cell biomedicine, in particular to umbilical cord mesenchymal stem cells with high PD-L1 expression, a culture method and application of the umbilical cord mesenchymal stem cells and a method for promoting PD-L1 expression through in-vitro energization. The umbilical cord mesenchymal stem cell in-vitro energizing inducer is based on a DMEM / F12 culture medium containing 3%-7% of human platelet lysis buffer, and further comprises the following components: a tumor necrosis factor alpha, interferon gamma, human serum albumin, transferrin, insulin, riboflavin, N-acetylcysteine and a vascular endothelial growth factor. The in-vitro energizing inducer reduces the content of a stimulating factor tumor necrosis factor alpha and interferon gamma, reduces immunogenicity abnormity caused by TNF-alpha, remarkably improves PDL1 expression and provides a basis for inflammation treatment. The influence of long-time stimulation on cell functions and activity is remarkably reduced through short-time factor-induced stimulation.

The utility model provides a hemoglobin and transferrin joint detection test strip and a reagent plate, and belongs to the technical field of biological detection. The colloidal gold pad is coated with a conjugate of an anti-human hemoglobin monoclonal antibody and colloidal gold and a conjugate of an anti-human serum transferrin monoclonal antibody and colloidal gold; the first detection line T1 is coated with at least one of an anti-human hemoglobin specific antibody or an anti-human serum transferrin specific antibody; the hemoglobin and transferrin combined detection test strip is coated with at least one second detection line T2 line of an anti-human hemoglobin specific antibody or an anti-human serum transferrin specific antibody, and is coated with an anti-mouse IgG antibody quality control region C line; the detection of serum protein and transferrin can be rapidly realized at one time, the use amount of detection materials in actual combats is saved, and the kit conforms to the operation habits of public security actual combats and is more suitable for difficult bloodstain detection.

The invention discloses a culture medium for suspension domestication culture of fibroblast stem cells and a domestication method. The culture medium comprises a low-serum culture medium and a serum-free culture medium; the low-serum culture medium comprises amino acid, vitamins, trace elements, serum and proliferation promoting factors; the serum-free culture medium comprises amino acid, trace elements, growth factors, hydrolysates, lipids and proliferation promoting factors; the proliferation promoting factor comprises any one or a combination of at least two of insulin, ethanolamine or transferrin. By means of the autonomous domestication method, the suspension cell domestication efficiency is improved, and the suspension cells obtained through domestication have the advantages of being high in density, good in stability, high in dispersity, high in multiplication speed and the like.

The invention provides a lipid composition, a culture medium and a method for multidirectionally differentiating human induced pluripotent stem cells to three germ layers and application of the lipid composition. The culture medium comprises a basic culture medium as well as a lipid combination, a cell protective agent, an antioxidant, a cell nutrition supplement, a non-essential amino acid supplement, an insulin-transferrin-selenium-ethanolamine supplement, a reducing agent, an anti-apoptosis agent and the like which are added into the basic culture medium, the method sequentially comprises the steps of embryoid body formation, multidirectional differentiation and re-paving and adherent culture. According to the lipid composition, the culture medium and the method, the embryoid body with regular form and high yield can be efficiently and stably formed, so that the differentiation of the induced pluripotent stem cells to the trigerm cells is promoted, and the expression level of the trigerm characteristic markers is remarkably improved. The method shows good repeatability and applicability in different induced pluripotent stem cell lines, and provides a reliable tool for standardized evaluation and downstream application of stem cell multidirectional differentiation potential.

This invention discloses a highly active duck embryo liver primary cell and its low-cost preparation method and applications. The preparation method includes: obtaining and pretreatment of duck embryo liver tissue, separation by complex enzyme digestion, preparation of cell suspension, primary cell culture, and maintenance of cell viability. The core of this method lies in the gentle digestion using a complex digestion solution composed of collagenase I, trypsin, and DNase I in specific weight ratios, followed by culture in a well-defined, non-commercial growth factor-free complete culture medium. This complete culture medium contains synergistic components such as DMEM / F12, fetal bovine serum, epidermal growth factor, hepatocyte growth factor, and an insulin-transferrin-selenium mixture. The duck embryo liver primary cells prepared by this invention exhibit high yield, high viability, and excellent liver-specific function, and can maintain their activity and function well after passage and cryopreservation.

The application relates to the technical field of stem cells, in particular to a mesenchymal stem cell culture system without heterogeneity and application thereof. The mesenchymal stem cell culture system without heterogeneity comprises a basic culture medium and an additive component, wherein the basic culture medium is DMEM / F12, and the additive component comprises: recombinant human transferrin, recombinant human epidermal growth factor, recombinant human basic fibroblast growth factor, recombinant human insulin, recombinant human interferon gamma, hydrocortisone, vitamin A, vitamin D2, vitamin B, human blood albumin, CTS KnockOut SR XenoFree, glutamine and dextran. The mesenchymal stem cell culture system without heterogeneity provided by the application improves the proliferation efficiency of primary culture and in-vitro expansion culture of mesenchymal stem cells, is high in safety, good in stability and suitable for large-scale popularization and application.

The invention belongs to the field of organic matter preparation, and particularly relates to a two-photon fluorescent nanoprobe for detecting superoxide anions in glioma as well as a preparation method and application of the two-photon fluorescent nanoprobe. A compound containing a naphthalene ring, a trifluoromethanesulfonate group and a cyanovinyl group is prepared as a nanoprobe, and then the nanoprobe is compounded with transferrin to form a delivery platform. The two-photon fluorescent nanoprobe for detecting superoxide anions in glioma and a delivery platform prepared from the probe have strong lysosome targeting imaging property and have an effect of crossing a blood brain barrier, and precise boundary description beyond traditional anatomical imaging is realized.

The invention relates to a serum-free culture medium and a preparation method and application thereof in the technical field of cell culture. The serum-free culture medium comprises a basic culture medium and the following components in final concentration: 5 to 15 ng / mL of dexamethasone, 200 to 600 ng / mL of hydrocortisone, 20 to 200 [mu] g / mL of transferrin, 0.2 to 1 mg / mL of human serum albumin, 40 to 70 [mu] M of beta-mercaptoethanol, 5 to 12 [mu] M of glutathione, 10 to 65 mg / L of ascorbic acid, 1 to 10 ng / mL of EGF, 1 to 20 ng / mL of IGF-1, 1 to 10 ng / mL of bFGF and 1 to 10 ng / mL of PDGF-BB. The basic culture medium is IMDM. According to the serum-free culture medium, an excellent culture effect can still be obtained by adding fewer culture medium components, the industrial production complexity is greatly reduced, the industrial production cost is reduced, and large-scale clinical requirements can be better met.

The application belongs to the technical field of culture medium, and particularly relates to a neural stem cell induction and differentiation culture medium, a preparation method and application thereof. The neural stem cell induction and differentiation culture medium comprises a basic culture medium and the following ingredients added in the basic culture medium: N-2 additive, transferrin, sodium selenite, iron ilex acid and fluridone. The neural stem cell induction and differentiation culture medium can significantly improve the effect of differentiating pluripotent stem cells into neural stem cells, and the culture medium does not add fetal bovine serum, thereby avoiding the risk of introducing heterologous serum carrying viruses and improving the safety of clinical application, and has a wide application prospect.