316 results about "Transferrin" patented technology

The invention discloses a serum-free adipogenic differentiation method for porcine adipose precursor cells, and relates to the technical field of mammalian animal cell differentiation.In the adipogenic differentiation process, an induced differentiation culture medium and a maintained differentiation culture medium are sequentially used, and the induced differentiation culture medium is composed of a basic culture medium and cell culture auxiliary factors; the cell culture auxiliary factor is composed of 66nmol / L insulin, 33 [mu] mol / L biotin, 17 [mu] mol / L pantothenic acid, 0.1 [mu] mol / L dexamethasone, 0.25 mmol / L IBMX, 1 [mu] mol / L rosiglitazone, 150 [mu] mol / L sodium oleate and 10 [mu] g / mL transferrin; the maintenance differential medium removes IBMX and rosiglitazone on the basis of inducing the differential medium. The serum-free adipogenic differentiation method for the porcine fat precursor cells, provided by the invention, is good in differentiation effect and high in repeatability.

The present disclosure is directed to bifunctional small molecules which contain a circulating protein binding moiety (CPBM) linked through a linker group to a cellular receptor binding moiety (CRBM) which is a membrane receptor of degrading cell such as a hepatocyte or other degrading cell. In certain embodiments, the (CRBM) is a moiety which binds to asialoglycoprotein receptor (an asialoglycoprotein receptor binding moiety, or ASGPRBM) of a hepatocyte. In additional embodiments, the (CRBM) is a moiety which binds to a receptor of other cells which can degrade proteins, such as a LRP1, LDLR, FcγRI, FcRN, Transferrin or Macrophage Scavenger receptor.

The invention discloses a serum-free culture medium for in-vitro proliferation of poultry muscle stem cells and application of the serum-free culture medium, and belongs to the technical field of cell engineering, a basic serum-free culture medium is DMEM-F12, a complete serum culture medium is a mixture of DMEM-F12 added with 10% of fetal calf serum and 1% of mycillin, and the volume ratio of a culture medium supplement to the basic culture medium is 1: 1000; the serum-free culture medium contains a supplement; the supplement comprises the following compositions: insulin, transferrin, fibroblast growth factor 2, epidermal growth factor, catalase, ethanolamine, vitamin B12, linoleic acid, linolenic acid, retinol acetate, tocopherol, L-alanine, L-proline, sodium selenite, sodium pyruvate, reduced glutathione, berberine hydrochloride and analogues thereof.

The present invention discloses a complete medium for culturing DF-1 cells, including DMEM basal medium 13.7g / L, insulin, non-essential amino acids, transferrin, glutathione, ascorbyl phosphate, sodium selenite, ethanolamine, hydrocortisone, and newborn calf serum. Wherein non-essential amino acids include glycine, alanine, proline, serine, and asparagine. A complete medium for culturing DF-1 cells provided by the present invention can well promote the proliferation of DF-1 cells, can achieve the same proliferation effect as high-concentration fetal bovine serum, and is suitable for the adherent culture of DF-1 cells; using low-concentration newborn calf serum, it is possible to greatly avoid the batch differences, unstable culture results, cytotoxicity, and high risk of carrying pathogens brought by the culture medium with high-concentration serum, narrow application range, expensive fetal bovine serum, and high culture cost; the components are simple, the culture medium preparation process is short in time consumption, the culture efficiency is high, the repeatability is good, and it is suitable for batch production.

To provide polypeptides, such as fibronectin type III (FN3) domains that can bind CD71, their conjugates, isolated nucleotides encoding the molecules, vectors, host-cells, and further to provide methods of making and using the same.SOLUTION: Provided are: polypeptides including specific sequences or an amino acid sequence that is at least 90% identical to an amino acid sequence of any combination thereof; or a polypeptide including specific sequences or an amino acid sequence of any combination thereof. In some embodiments, an FN3 domain does not naturally exist. In some embodiments, the FN3 domain binds to human CD71 at a site on CD71 that does not compete with transferrin binding to CD71.SELECTED DRAWING: None

The invention provides a transferrin modified targeted lipid nano bubble as well as a preparation method and application thereof, and relates to the technical field of synthesis of ultrasonic medical imaging contrast agents. The transferrin modified targeted lipid nano bubble provided by the invention has a bubble-like structure; the shell of the transferrin modified targeted lipid nano bubble is made of single-layer phospholipid; the surface of a shell of the transferrin modified targeted lipid nano bubble is coupled with biotinylated TF through streptavidin; and perfluoropropane gas is wrapped in the transferrin modified targeted lipid nano bubble. The transferrin modified targeting lipid nano bubble provided by the invention has good stability and strong targeting property, and can target ferroptosis cells.

The invention relates to the technical field of cell culture. The invention provides a mesenchymal stem cell culture medium without animal-derived components and application of the mesenchymal stem cell culture medium. The culture medium comprises a basic culture medium and an additive, the additives comprise FGF-2, IGF-1, glutamine, folic acid, serotonin, cholesterol, arachidonic acid, an HEPES buffer solution, sodium bicarbonate, SCF, TGF-beta, PDGF, ascorbic acid and transferrin. The mesenchymal stem cell culture medium disclosed by the invention is completely free of animal-derived components, and meets the requirements of FDA / EMA clinical-grade cell therapy products. The culture medium can support high-density amplification of mesenchymal stem cells, the activity of passage cells is effectively improved, and the amplification time is shortened. The method has remarkable significance in improving the amplification efficiency of the mesenchymal stem cells and promoting and applying factory growth.

The invention discloses a kit for detecting transferrin and application of the kit, and belongs to the technical field of transferrin content detection. The kit comprises a PBA-SiO2 (at) Fe3O4 magnetic affinity probe and an Ab-Au, Pt, Cu (at) PDA nano enzyme immune probe, and when the transferrin exists, the PBA-SiO2 (at) Fe3O4 magnetic affinity probe and the Ab-Au, Pt, Cu (at) PDA nano enzyme immune probe are combined with the transferrin to form a sandwich structure similar to a sandwich. A substrate generates an oxidation product 3, 3 ', 5, 5'-tetramethyl benzidine diimine with color development capability by utilizing the catalytic performance of Ab-Au, Pt, Cu (at) PDA nano-enzyme and the Raman enhanced activity of Au, Pt and Cu metals, and a novel transferrin multi-mode sensing platform which is rapid, sensitive, high in selectivity and high in reliability is established; and in combination with visual sensing analysis, the transferrin in a sample can be rapidly and semi-quantitatively detected on site. The method is expected to be applied to on-site rapid detection of low-concentration transferrin in samples such as sweat, blood and urine.

The invention provides a skin care composition containing stem cell extract as well as a preparation method and application of the skin care composition, and belongs to the technical field of cosmetics. The method comprises the following steps: mixing and culturing the extracted urine-derived stem cells and pilose antler stem cells in a DMEM culture medium added with epidermal growth factors, cholera toxin, penicillin, streptomycin, transferrin, IL-2 and IL-15, then separating a culture solution to obtain mixed exosomes, mixed stem cells and extracellular matrixes, preparing the mixed stem cells into stem cell lipidosome, and preparing the stem cell lipidosome. Embedding the stem cell extract and the mixed exosome into titanium dioxide / silicon dioxide microspheres, adding into an extracellular matrix, and uniformly dispersing to obtain the skin care composition containing the stem cell extract. The skin care composition containing the stem cell extract prepared by the invention can obviously prolong the preservation period of stem cells and exosomes and improve the repair and improvement of the stem cells and the exosomes on skin, and the prepared composition has better effects of resisting aging, whitening, promoting skin elasticity, resisting wrinkles and the like, and has a wide application prospect.

The invention discloses a pharmaceutical composition for delaying oocyte senescence and application thereof, and relates to the technical field of biological medicine, and the pharmaceutical composition comprises alpha-ketoglutaric acid, a bicarbonate buffer solution, an energy metabolism substrate, polyvinyl alcohol, hormone, a growth factor and insulin-transferrin-selenium. The pharmaceutical composition for delaying oocyte aging disclosed by the invention is beneficial to improving the maturation rate of oocytes, reducing the cytoplasm fragmentation rate and the cleavage fragmentation rate, and improving the cleavage rate and the blastocyst rate; according to the invention, the content of beta-galactosidase is reduced, the function of mitochondria in the aging process after ovulation of the mature oocyte is improved, the membrane potential of the mitochondria in the aging process after ovulation of the mature oocyte is stabilized, the activity of the mitochondria is improved, and the normalization of RNA methylation modification in the aging process after ovulation of the mature oocyte is promoted.

The present invention discloses a low - serum medium and its application in the preparation of cultured meat from Larimichthys crocea cells, which is also applicable to other marine or freshwater fish, belonging to the field of bioengineering technology. The low - serum medium comprises a basal medium, fetal bovine serum accounting for 30 - 80 μL / mL, and exogenous additives. The exogenous additives include human serum albumin, ascorbic acid, basic fibroblast growth factor polypeptide, human holo - transferrin, lysophosphatidic acid, and human recombinant IGF - 1. Based on the DMEM / F12 medium, the low - serum medium provided by the present invention adds six substances that promote cell growth, reduces the dependence of satellite cells on fetal bovine serum, effectively reduces the usage amount of fetal bovine serum, and saves the culture cost. Compared with the general - type medium (DMEM high - glucose, adding 10% FBS), the low - serum medium provided by the present invention has an equivalent effect on the maintenance and proliferation ability of cell stem cells.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium which is composed of a basic culture medium and an additive, and the basic culture medium is DMEM / F12. The additive is prepared from albumin, transferrin, insulin, a fibroblast growth factor (FGF), an epidermal growth factor (EGF), dexamethasone, glutamine, ascorbic acid, recombinant human III type collagen and recombinant human XVII type collagen. According to the UCMSCs obtained by culturing the umbilical cord mesenchymal stem cells by using the serum-free culture medium, the cellular morphology and surface marker expression are maintained; the umbilical cord mesenchymal stem cell serum-free culture medium has the advantages that the cell proliferation capacity, the monoclonal performance capacity and the three-line differentiation capacity are enhanced, the umbilical cord mesenchymal stem cell serum-free culture medium only contains 12 raw materials, the compounding process is simple, the cost is low, the raw materials of the umbilical cord mesenchymal stem cell serum-free culture medium are clear in quality, and the umbilical cord mesenchymal stem cell serum-free culture medium can be used for culturing clinical-grade UCMSCs.

The invention provides a special culture medium for breast cancer organoid and a culture method thereof, and belongs to the technical field of primary cell culture. The special culture medium for the breast cancer organoid is composed of a basic culture medium and a culture additive, and the basic culture medium is a DMEM (Dulbecco Modified Eagle Medium) / F12 culture medium. The culture additive is prepared from an epidermal growth factor, a basic fibroblast growth factor, a keratinocyte growth factor, a fibroblast growth factor 10, insulin, transferrin, sodium selenite, A83-01, SB202190, an insulin-like growth factor 1, B27, nicotinamide and N-acetylcysteine. According to the culture medium, the culture cost is reduced, and meanwhile, the stability of tumor cell genomes in the culture process is improved.

The invention relates to the technical field of animal vaccines, in particular to a recombinant porcine epidemic diarrhea virus S1 protein and transferrin fusion protein as well as a preparation method and application thereof. According to the present invention, the SpyTag sequence is inserted into the S1 gene sequence, the eukaryotic cell HEK293 is adopted to express the protein, such that the obtained recombinant S1 protein has sufficient glycosylation modification, and when the recombinant S1 protein is mixed with the recombinant transferrin containing the SpyCatcher sequence, the K31 on the SpyTag and the N117 on the SpyCatcher are subjected to spontaneous condensation to form the isomeric peptide so as to display the antigen on the transferrin 24 polymer surface; the technical system not only maintains the natural conformation of the virus antigen protein, but also has the advantage of stimulating the innate and adaptive immune response functions of the host, and has a better protection effect on the host.

The present invention relates to the field of cell culture, and particularly to a serum-free induction adipogenic differentiation medium for fish stem cells and its application. It is composed of a basal medium and a combination of cell culture cofactors; the basal medium is one of DMEM and DMEM / F12 medium; the combination of cell culture cofactors consists of oleic acid, soybean lecithin, vitamin C phosphate, β-cyclodextrin, transferrin, saikosaponin A, bakuchiol, dehydroabietic acid, and phenyl lactic acid. By adding the combination of cell culture cofactors, the present invention replaces the serum in the traditional adipogenic induction differentiation medium for preadipocytes and the core component dexamethasone IBMX in the traditional adipogenic differentiation "cocktail", and obtains a medium suitable for serum-free induction adipogenic differentiation of fish stem cells, which not only solves the bottleneck problems of the prior art, but also provides reliable technical support for the large-scale production of cell-cultured fish meat.

The invention provides a recombinant pichia pastoris engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of biochemistry and genetic engineering, the recombinant pichia pastoris engineering bacterium is a pichia pastoris strain in which an FDH1 gene as shown in SEQ ID NO.1 is subjected to targeted knockout, and a DAK2 gene as shown in SEQ ID NO.3, an SHM2 gene as shown in SEQ ID NO.5, an FBA1 gene as shown in SEQ ID NO.7 and an MTD1 gene as shown in SEQ ID NO.9 are inserted; the recombinant pichia pastoris engineering bacterium provided by the invention can obviously improve the yield of recombinant protein, and particularly, the yield of proteins such as recombinant human transferrin, recombinant human albumin, recombinant human basic fibroblast growth factors and the like is greatly improved.

The invention relates to the field of cell culture, in particular to a serum-free induced adipogenic differentiation culture medium for fish stem cells and application of the serum-free induced adipogenic differentiation culture medium. The culture medium consists of a basic culture medium and a cell culture auxiliary factor combination, the basic culture medium is one of a DMEM (Dulbecco's Modified Eagle Medium) and a DMEM / F12 culture medium; the cell culture auxiliary factor combination is prepared from oleic acid, soya bean lecithin, vitamin C phosphate, beta cyclodextrin, transferrin, saikoside A, psoralen flavanone methyl ether, dehydroabietic acid and phenyllactic acid. The culture medium suitable for serum-free induced adipogenic differentiation of fish stem cells is obtained by adding a cell culture auxiliary factor combination to replace serum in a traditional adipose precursor cell adipogenic induced differentiation culture medium and dexamethasone IBMX serving as a core component of traditional adipogenic differentiation cocktail, the bottleneck problem in the prior art is solved, and the culture medium is suitable for serum-free induced adipogenic differentiation of fish stem cells. And reliable technical support is also provided for large-scale production of cell culture fish meat.

The invention provides an NK (Natural Killer) cell culture medium, which is prepared by adding the following components into a 1640 culture medium: 8 to 11 percent v / v fetal calf serum, 18 to 22 mg / mL of recombinant human transferrin, 40 to 60 mu M of 1, 3-dicaffeoylquinic acid, 25 to 35 mu M of aceglutamide, 900 to 1100 IU / mL of human recombinant IL-2, 45 to 55 IU / mL of human recombinant IL-15, 45 to 55 IU / mL of human recombinant IL-21, 50 to 200 U / mL of penicillin, 50 to 150 mu g / mL of streptomycin and 1 to 2 mg / mL of more than 95 percent of polyporus gossypii polysaccharide. The NK cell culture medium provided by the invention can effectively improve the proliferation speed of NK cells and the killing effect on various tumor cells.

The invention discloses a culture medium and a culture method of appendix mucinous tumor organoid. The culture medium of the appendix mucinous tumor organoid is prepared by adding the following components with final concentration into a basic culture medium: 2-8nM of NRG1 and 1-2mu g / ml of R-Spondin1, 90 to 110 [mu] g / ml of Noggin; 70 to 80 [mu] g / ml of Wnt-3a; eGF with a concentration of 45 to 55 ng / ml; the concentration of the FGF-10 is 90 to 110 ng / ml; 1X B to 27TM; 4 to 6 mM of nicotinamide; 4 to 6 nM of prostaglandin E2; 9 [mu] g / ml to 11 [mu] g / ml of Transferrin human; 4-6 [mu] g / ml of insulin; 4 to 6 nM of gastrin I; 9 to 11 [mu] M of Y27632; 1 to 2 [mu] M of A83-01; 4 to 6 [mu] M of SB202190; and 2 to 3 [mu] M of CHIR999021. The culture medium is high in induction rate.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium, which is composed of a basic culture medium and additive components, and the additive components comprise, by final concentration, 5-20 [mu] g / mL of astragalus polysaccharide, 0.5-2.0 [mu] g / mL of salidroside, 0.1-0.5 [mu] g / mL of tanshinone IIA, 0.1-0.3 mg / mL of sodium pyruvate, 5-15 mM of D-ribose, 3-6 mg / mL of recombinant human albumin, and 5-10 [mu] g / mL of recombinant transferrin; the invention also discloses a method for culturing umbilical cord mesenchymal stem cells under the hypoxia condition by using the serum-free culture medium. The method comprises the steps of cell inoculation, culture under the hypoxia condition, subculture, cell passage and the like. The astragalus polysaccharide, the salidroside, the tanshinone IIA, the sodium pyruvate, the recombinant human albumin and the recombinant transferrin are added into the serum-free culture medium, so that nutrition supply and function maintenance of cells under a low-oxygen condition are guaranteed, the proliferation rate and the differentiation capacity of the cultured cells are improved, the apoptosis rate of the cells is reduced, and the survival rate of the cells is increased. Wide application prospects are realized in the fields of cell culture, regenerative medicine and the like.

Provided is a serum-free medium that is composed of a fewer number of constituent components and is less expensive.　The serum-free medium comprises Dulbecco's modified Eagle's medium (DMEM), albumin (BSA), and insulin-transferrin-selenium-ethanolamine (ITS-X). The serum-free medium is suitable for the culturing of mouse ES cells that are naïve pluripotent stem cells, and bears comparison with the most superior medium on the market.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

This invention discloses a NK cell culture kit, its culture method, and its application. The kit includes an amplification medium, a high-efficiency induction medium, an NK-A coating solution, an NK-B mixture, and an NK-C mixture. The amplification medium contains basal medium, NAD+, human serum albumin, transferrin, β-glucan, glutathione, β-mercaptoethanol, and linoleic acid. The high-efficiency induction medium, in addition to the amplification medium components, contains soybean peptides, nicotinamide, inulin, and N-acetyl-L-cysteine. The NK-A coating solution contains heparin sodium, monoclonal antibodies, and antibodies. The NK-B mixture contains Inbakicept, IL-2, and IL-15. The NK-C mixture contains linolenic acid, IL-2, and IL-18. The Inbakicept factor in the kit can activate NK cells and enhance their cytotoxicity; linolenic acid can inhibit T cell proliferation, increase NK cell purity, and enhance cell efficacy.

The invention belongs to the technical field of molecular biology, and relates to an application of transferrin (TF) as an internal reference protein in preparation of a reagent for detecting exosome proteins of neurodegenerative diseases. The TF can maintain a stable expression level in nerve cells, brain tissues, blood plasma and serum-derived exosomes of a subject suffering from neurodegenerative diseases, including Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease, and also including neurodegenerative diseases such as Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease. The TF expression variation coefficient is obviously lower than that of a common exosome marker, and the expression level of TF has stronger correlation with the total protein amount of the exosome and is not influenced by external factors such as cell inflammation stress. The TF as the internal reference protein has more excellent performance, can help to more accurately reflect the total loading amount of the sample, and provides a more stable and reliable standardized tool for the field of exosome research.

The present disclosure relates to bifunctional small molecules comprising a circulating protein binding moiety (CPBM) linked to a cell receptor binding moiety (CRBM) via a linker group, said (CRBM) being a membrane receptor of a degrading cell, such as a hepatocyte or other degrading cell. In certain embodiments, the (CRBM) is a moiety that binds to an asialoglycoprotein receptor of a hepatocyte (an asialoglycoprotein receptor binding moiety, or an ASGPRBM). In further embodiments, the (CRBM) is a moiety that binds to a receptor of another cell capable of degrading a protein such as LRP1, LDLR, Fc [gamma] RI, FcRN, transferrin or macrophage scavenger receptor.

The invention discloses an NK cell serum-free medium, the serum-free medium comprises a basic medium and an additive, the additive comprises the following components: recombinant human transferrin, recombinant human insulin, human serum albumin, growth factors, glucose, trace elements, tacrolimus and mevalonic acid, the serum-free culture medium comprises the following components in percentage by weight: 85% of a basic culture medium, 3% of recombinant human transferrin, 3% of recombinant human insulin, 2% of human serum albumin, 2% of growth factors, 2% of glucose, 1.5% of trace elements, 1% of tacrolimus and 0.5% of mevalonic acid; according to the serum-free culture medium for the NK cells, growth and differentiation of the NK cells can be adjusted by adding a proper amount of tacrolimus, meanwhile, a proper amount of mevalonic acid is matched, raw materials of active substances can be provided for the cultured NK cells, growth and development of the NK cells or expression of specific functions are facilitated, and therefore the survival rate and the amplification number of the NK cells can be increased.

The invention discloses a duck skeletal muscle satellite cell serum-free myoblast induced differentiation culture medium and an induction method and application, and relates to the technical field of animal cell differentiation. The cell culture auxiliary factor is composed of 0.1 [mu] mol / L of insulin, 1 [mu] mol / L of dexamethasone, 1 [mu] g / mL of linoleic acid, 10 [mu] g / mL of transferrin and a 1 * GlutaMAX supplement. The duck skeletal muscle satellite cell serum-free myoblast induced differentiation culture medium provided by the invention does not contain any serum component, has definite chemical components and good differentiation effect, and is convenient for promoting the industrialization process of cell culture meat.

The invention relates to the technical field of cell culture media, in particular to a sheep testis somatic cell culture medium and a culture method.The sheep testis somatic cell culture medium is prepared from, by weight, 10-15 parts of MEMS alpha, 4-7 parts of KSR, 2-4 parts of sodium chloride, 3-5 parts of recombinant human transferrin, 1-3 parts of ferric citrate, 2-4 parts of L-glutamine, 5-10 parts of water and 4-7 parts of hydroxyapatite. According to the cell culture medium, MEM and KSR are adopted as matrix raw materials, sodium chloride and other auxiliaries are added, the hydroxyapatite agent is matched, the performance change of the cell culture medium on the survival rate and the survival amount of testicular somatic cells is enhanced, meanwhile, hydroxyapatite serves as a matrix of the hydroxyapatite agent, the performance of the cell culture medium is improved through a specific modification solution, and the cell culture medium is prepared. And the function of hydroxyapatite blended kaolin in the system is optimized, so that the functional effect of the cell culture medium is enhanced, and the performance of the product is further improved.

The present disclosure relates to a method for assessing the effectiveness of a treatment with lanifibranor in a patient with a liver disease, the method comprising: a) in vitro measuring levels of a combination of biomarkers consisting of adiponectin, ferritin, MMP9 and transferrin in a biological sample from the patient; b) assessing the effectiveness of the treatment with lanifibranor as a function of the levels measured in step a).