7 results about "Reproductive cells" patented technology

The invention provides a construction method of a viral reproductive dysfunction model based on spermatogonial stem cells and application of the model, and belongs to the cross technical field of biotechnology and veterinary medicine. The construction method comprises the following steps: placing spermatogonial stem cells in a spermatogonial stem cell culture medium for culture, and infecting the spermatogonial stem cells with bovine viral diarrhea viruses to obtain the viral reproductive function disorder model based on the spermatogonial stem cells. In order to solve the problems that in the prior art, a BVDV reproductive injury mechanism is unknown, and an effective research model is lacked, the reproductive stem cells are used for simulating direct injury of BVDV to a male reproductive system for the first time, and the defect that a traditional model (such as MDBK cells) cannot reflect specific biological reaction of reproductive cells is overcome; meanwhile, experiments show that the model constructed by the invention can support standardized processes of virus strain typing and drug screening, the research and development efficiency is remarkably improved, and the experiment cost is reduced.

The application belongs to the technical field of medicine, and discloses application of uridine-5' monophosphate and a compound thereof in preparation of a medicine for enhancing the proliferation ability of male reproductive cells. It is found for the first time that the uridine-5' monophosphate has the proliferation ability on the male reproductive cells, and the proliferation effect is best when the concentration of the uridine-5' monophosphate is 10 μM, the proliferation effect on the mouse TM3 cells is increased by 52.87% compared with a control, and the proliferation effect on the mouse TM4 cells is increased by 53.57%. In addition, the uridine-5' monophosphate has a synergistic ability when being compounded with xylonic acid lactone, and further improves the proliferation effect on the TM3 cells, thereby laying a foundation for treatment of male reproductive system diseases in the future.

A contraceptive device is provided including an occluding component for deployment in a tubal lumen of a reproductive organ. The device includes an expandable frame reversibly expandable from a collapsed state to an expanded state and a barrier supported by the expandable frame. In the expanded state the barrier is disposed between the expandable frame and walls of the lumen to inhibit in-growth of cellular material into and onto the occluding component, and additionally the barrier is urged against walls of the tubal lumen to define at least two tube lumen sealing areas, disposed perpendicular to a longitudinal axis of the walls of the tube lumen such that (i) the expandable frame is disposed between the two tubal lumen sealing surface, and (ii) passage of reproductive cells through the expandable frame, and through the tube lumen is blocked, therefore, creating a the complete blockage for liquids from a first part of the tube to a second part of the tube, downstream the first part. Other applications are also described.

The invention discloses a low-temperature preservation and resuscitation device for reproductive medicine, and belongs to the technical field of low-temperature preservation and resuscitation. The resuscitation assembly is connected with the top of the base so as to resuscitate the sample test tube filled with the germ cells; the low-temperature preservation assembly is detachably arranged at the top of the resuscitation assembly and is communicated with the resuscitation assembly so as to perform low-temperature preservation on the sample test tube filled with the germ cells; wherein a switch assembly is arranged at the communicating position of the low-temperature preservation assembly and the recovery assembly, and opening and closing of the communicating position are achieved; and the driving assembly is connected to the low-temperature preservation assembly, so that the sample test tubes placed on the driving assembly are fed into the resuscitation assembly. Through integrated and automatic design, seamless transfer of the sample between preservation and recovery is realized, temperature fluctuation and pollution risks are reduced, the operation efficiency, the standardization degree and the cell survival rate are improved, and meanwhile, the cost is reduced.

This invention discloses an antibody for prolonging ovarian function, a culture medium, a method for inhibiting primordial follicle activation, and its application. The antibody is a CD138 antibody. In the technical solution provided by this invention, the CD138 antibody can precisely regulate the transition rhythm of primordial follicles from a quiescent state to a developing state by targeting and inhibiting the primordial follicle activation signaling pathway. This regulatory mechanism can reduce the ineffective activation and loss of follicles in early reproductive years, allowing more primordial follicles to remain dormant, thereby prolonging the physiological lifespan of the follicle pool. This provides biological protection for the even distribution of reproductive cell resources throughout the reproductive cycle in female individuals, effectively avoiding the risk of infertility or premature ovarian failure caused by premature follicle depletion.

The application discloses a RNAi interference fragment targeting a donkey Zfy gene, a carrier and a preparation method and application thereof, and relates to the technical field of animal molecular genetics, wherein the RNAi interference fragment comprises RNAi-3-F and RNAi-3-R, and the nucleotide sequences are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The RNAi interference fragment provided by the application can target and silence Y chromosome sex determination related genes in male donkey Y spermatogenesis stage key reproductive cells, hinder and interfere with the normal development and physiological functions of Y sperm, regulate the key genes of Y sperm development through specific silencing, realize sex regulation before fertilization, greatly improve the proportion of female individuals of offspring donkeys, and the scheme does not damage target sperm cells and does not reduce sperm activity, and meanwhile, the scheme has the advantages of simple operation and low production cost.

Provided are a cryopreservation liquid for bovine reproductive cells such as bovine sperms and a cryopreservation method thereof. Adopted is a cryopreservation liquid comprising: 0.3 to 0.9 w / w % of an amphoteric polyelectrolyte (an antifreeze polyamino acid), which is ε-poly-L-lysine wherein 50 to 99 mol % of amino groups of the ε-poly-L-lysine are blocked as carboxylated by having been reacted with the succinic anhydride; and glycerol, as dissolved in a physiological solution.