43 results about "Reproductive cells" patented technology

Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian, avian, or piscian reproductive sperm cells. The compositions comprise a suitable reproductive cell media and a transforming growth factor, an insulin-like growth factor, or zinc, and, optionally, inositol, transferrin, or fructose, or combinations thereof.

The present invention relates to a method and device for male contraception. In one aspect, the method and device provide temporal and reversible infertility of mammal males without suppressing spermatogenesis. In an aspect, a method of contraception for use with reproductive cells that pass through a tube in a body is described in which energy is applied through the tube wall to some of the reproductive cells that pass through the tube to reduce fertilization ability of the some of the reproductive cells. In another aspect, the device is adapted for use with reproductive cells that pass through a tube in a body, and includes an energy transmitter that delivers energy to the reproductive cells that pass through the tube in the body.

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer; and cryopreservation.

The rape transgenic method of the present invention adopts the in-situ reproduction organ as transgenic receptor. On the basis of cytological observation of pollinated rape style and ovary, the transforming time is determined. The transformation includes cutting off the style head, dropping exogenous DNA solution, transforming the reproductive cell with the gene carrying exogenous gene and glufosinate resistance marker gene to obtain transgenic T1 generation zygocyte in the transformation period; and screening the T1 generation seedling with low concentration glufosinate solution to obtain transgenic positive rape plant. The present invention has transgenic efficiency of 2-10 %, high transformation flux, low cost, less work, no genotype dependence and other advantages.

Methods are provided for the ex vivo manipulation of cells, stem cells and other reproductive cells, by manipulating the cells in a container or device comprising an elastic substrate, wherein the substrate has an elasticity that mimics the elasticity of a native microenvironment of the cell.

The invention provides a testicle cryopreservation agent for marine fishes and a preparation method of spermatogonium. The preparation method of spermatogonium comprises the following main steps: preparing a cryopreservation agent formula suitable for sciaenops ocellatus; establishing a testicle cryopreservation and unfreezing method; preparing the transplantable spermatogonium. The invention establishes a cryopreservation method for the spermatogonium of the sciaenops ocellatus and technical guarantees are provided for a reproductive cell transplanting technology. According to the testicle cryopreservation agent for the marine fishes and the preparation method of the spermatogonium, a donor source for transplanting reproductive cells is expanded; a testicle is cryopreserved for a long period and can be used according to requirements; guarantees are provided for the donor source for transplanting the reproductive cells and an application range of transplanting of the reproductive cells of fishes is greatly widened.

The invention discloses a slide climbing method of PGCs (primordial germ cells) and belongs to the biotechnology field. The slide climbing method comprises the steps as follows: purifying the PGCs; washing the purified PGCs with a PBS (phosphate buffer solution) for three times, and centrifugally collecting the cells; selecting and putting 100-200 cells in PBS droplets on a glass slide under a microscope for washing for one time; selecting and putting the cells in a paraformaldehyde solution with the concentration of 4% on the glass slide for washing for one time; selecting and putting the cells on paraformaldehyde droplets with the concentration of 4% on the glass slide for fixation for 10-30 min; and removing the paraformaldehyde with the concentration of 4% through adsorption, and finishing slide climbing of the cells while fixing the cells. According to the slide climbing method, the problem that the purified PGCs are difficult to climb the glass slide is solved, and the time and the cost are saved.

The invention discloses a method to reprogram fibroblasts into Sertoli cells in vitro and application of the method. The method comprises the steps of introducing into fibroblasts, a fluorescent protein reporter system that specifically expresses in Sertoli cells; introducing a substance that increases NR5A1 protein expression and/or activity; adding G418, and culturing for 3-7 days; discarding anaqueous phase, adding a medium to culture fibroblasts, and continuing to culture for 3-7 days; isolating Sertoli cells from the culture system. Experiments prove that the cells prepared by the abovemethod fully attain the characteristics of Sertoli cells, such as ability to attract endothelial cells, ability to form lipid droplets, ability to interact with reproductive cells, ability to inhibitthe growth of lymphocytes and occurrence of interleukin, and ability of immune privilege and the like. The method has important applicable value in the fields, such as treatment of infertility, allograft, and cell therapy.

The invention belongs to the technical field of cell culture, particularly discloses an automatic cell culture device capable of simulating internal environments of organisms. The automatic cell culture device comprises a laminar flow sterile sealed chamber. An internal environment simulation tissue and organ culture device and a target cell induction culture device are arranged in the sealed chamber. The automatic cell culture device can simulate the internal environments including a low-oxygen low-permeability environment for culturing embryonic stem cells, a testicular internal environment during maturation of germ cells, a thymus environment for inducing maturation of T cells, a cerebrospinal fluid environment for inducing differentiation of neural stem cells and tissue and organ environments such as livers, pancreas, bone marrow and tumors for inducing differentiation and maturation of corresponding stem cells, and cultures specific high-quality cells in an induction mode to meet needs of cell basic researches and applications. Besides, the simulated internal environments provide a basis for researching an organism cell growth, differentiation and maturation mechanisms. Therefore, the improvement of cell culture quality is facilitated, repeatability of cytology researches is remarkably increased, cost of cell culture is saved, clinic transformation and application of the cell culture technology are quickened, and a good experiment platform is provided for scientific research basic workers.

The invention mainly relates to the field of aquaculture technologies, and discloses a method for artificially breeding parabramis pekinensis. The method includes selecting parent fish; cultivating the parent fish; artificially inducing spawning; carrying out artificial insemination; carrying out artificial hatching. The method has the advantages that the method is simple, the reproduction periods of the parabramis pekinensis can be brought forward by 12-15 days, the reproductive capacity of the parabramis pekinensis can be improved and can reach to 8021 roes, and economic income can be increased by 17.3%; the parent fish of the parabramis pekinensis is cultivated in indoor environments, special parent fish feeding agents are splashed, growth conditions required by the female and male parent fish can be regulated, water can be purified, gonad development can be promoted, the disease resistance of the parent fish and the viability of reproductive cells can be enhanced, the fertilization rate can be increased and is 92%, debonding is carried out after fertilization is carried out, debonded liquid is extracted and then is fermented by lactobacillus acidophilus, has appropriate pH (potential of hydrogen), is antibacterial and insecticidal, contains abundant nutrition, is safe and healthful and is debonded quickly, the viability of cells of fertilized roes can be enhanced, and the hatching rate can reach to 91%.

The invention relates to external physical therapy liquid for treating thyrophyma. The external physical therapy liquid comprises the following raw materials in parts by weight: 25-35 parts of ternate buttercup root, 15-20 parts of stiff silkworm, 10-15 parts of semen brassicae, 8-10 parts of gamboge, 5-10 parts of ephedra, 8-10 parts of rhizoma polygonati, 20-24 parts of thunberg fritillary bulb, 10-13 parts of kelp, 15-25 parts of platycodon grandiflorum, 8-10 parts of selfheal, 12-18 parts of glossy privet fruit, 10-15 parts of caulis spatholobi, 5-7 parts of eclipta alba, 8-15 parts of sculellaria barbata and 12-18 parts of white paeony root. The several traditional Chinese medicines in compatibility have obvious curative effects of thyrophyma, and the effective rate is 92%. Meanwhile, the traditional Chinese medicines have obvious curative effects for treating scrofula and goiter. The external physical therapy liquid disclosed by the invention has effects of inhibiting tumor cell growth, proliferation and metastasis, killing cancer cells, repairing damaged normal cells and new reproductive cells, easing pain, relieving depression, obviously relieving pain, improving unhealthy emotions and alleviating uncomfortable symptoms of the whole body and also has the effects of promoting appetite, improving sleep, balancing nutrition and invigorating health effectively.

The invention relates to an anti-seasonal reproduction method of crucian carp. The reproduction of crucian carp in winter includes the following steps: (1) disinfecting a temporary culture pond and ahatching tool; (2) preparing a parent fish culture pond; (3) parent fish and breeding thereof; (4) putting parent fish in the pond for temporary culture according to a male-to-female ratio and corresponding parent fish screening criteria; (5) spawning induction; and (6) fertilization and hatching. The reproduction of crucian carp in summer includes the following steps: (1) disinfecting a temporaryculture pond and a hatching tool; (2) parent fish and breeding thereof; (3) putting parent fish in the pond according to a male-to-female ratio; (4) spawning induction; and (5) fertilization and hatching. Different reproduction methods are set for different seasons, the water temperature is adjusted reasonably, and the proportion of oxytocic is set creatively. Thus, the parent fish are in a physiological state of normal ovulation or spermiation, and the vitality of reproductive cells is improved. Through the innovation of the breeding mode and the screening criteria, the problem of egg agingof female fish is solved.