43 results about "Reproductive cells" patented technology

Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian, avian, or piscian reproductive sperm cells. The compositions comprise a suitable reproductive cell media and a transforming growth factor, an insulin-like growth factor, or zinc, and, optionally, inositol, transferrin, or fructose, or combinations thereof.

A device for occluding a body lumen, and particularly contraceptive or sterilization device for occluding a reproductive tract or lumen to prevent the passage of reproductive cells through the tract or lumen, generally comprising a tubular member, and a mesh member, transversely disposed on the tubular member lumen. The mesh member is permeable to allow for tissue ingrowth, which produces a tissue impregnated mesh occluding the body lumen. The occluding device of the invention can be used in the fallopian tubes of a female patient, the vas deferens of a male patient, or other body lumen.

The present invention relates to a method and device for male contraception. In one aspect, the method and device provide temporal and reversible infertility of mammal males without suppressing spermatogenesis. In an aspect, a method of contraception for use with reproductive cells that pass through a tube in a body is described in which energy is applied through the tube wall to some of the reproductive cells that pass through the tube to reduce fertilization ability of the some of the reproductive cells. In another aspect, the device is adapted for use with reproductive cells that pass through a tube in a body, and includes an energy transmitter that delivers energy to the reproductive cells that pass through the tube in the body.

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer; and cryopreservation.

Extracts obtained from the genus Hippophae or other plant sources or compositions produced by chemical or molecular biological techniques each having in common certain moieties in amounts effective when combined with reproductive cells to reduce the loss of function.

The rape transgenic method of the present invention adopts the in-situ reproduction organ as transgenic receptor. On the basis of cytological observation of pollinated rape style and ovary, the transforming time is determined. The transformation includes cutting off the style head, dropping exogenous DNA solution, transforming the reproductive cell with the gene carrying exogenous gene and glufosinate resistance marker gene to obtain transgenic T1 generation zygocyte in the transformation period; and screening the T1 generation seedling with low concentration glufosinate solution to obtain transgenic positive rape plant. The present invention has transgenic efficiency of 2-10 %, high transformation flux, low cost, less work, no genotype dependence and other advantages.

A device and method of using the device for contraception or sterilization and particularly for reversible contraception by occluding a reproductive lumen to prevent the passage of reproductive cells through the lumen for a desired period of time until the patient wishes to become fertile again and then be reopened. The occluding member preferably comprises a tubular framework formed from a shape memory material configured to be implanted in a reproductive lumen. The occluding member is implanted within a body lumen, secured to the wall of the reproductive lumen and then collapsed to collapse the wall and occlude the lumen. Alternatively, the occluding member may be collapsed upon a solid plug. The closure of the reproductive lumen may be reversed by introducing a balloon catheter and by a series of inflations of the balloon reexpanding the collapsed occluding member or by removing the plug. The occluding member and the plug may be configured to facilitate endothelialization, to provoke an inflammatory responses or to deliver a drug.

Methods are provided for the ex vivo manipulation of cells, stem cells and other reproductive cells, by manipulating the cells in a container or device comprising an elastic substrate, wherein the substrate has an elasticity that mimics the elasticity of a native microenvironment of the cell.

The invention discloses a new method for improving transgenic efficiency of animals. In the transgenic method, the sperm cells and the oocytes are used as vectors, through improvement of the method, the exogenous genes are respectively led to the sperm cells and the oocytes and the exogenous genes carried by the sperm cells and the oocytes are further integrated in the embryos through fertilization process, thus improving the efficiency of leading the exogenous genes to the nuclei and then realizing high efficiency expression. The method is characterized by respectively transfecting female and male reproductive cell systems by adopting a point injection method before the female and male mice mate with each other and then selecting the male mice 1-5 days and 35-40 days after surgery and the female mice 1-7 days after surgery to mate with each other to obtain the transgenic mouse offspring. By adopting the method, transgenosis of sheep is explored, the transgenic efficiency of big mammals can be improved and a new approach is provided for breeding in the animal husbandry. In the method, a transgenic system adopting female and male reproductive cells for joint mediation is explored.

A method of localising reproduction assisting hyaluronic acid to reproductive cells surfaces by covalently linking it to lipids is disclosed.

The invention discloses a method for rapid separation and line establishment of chicken gonad primordial germ cells. The method comprises: separating and purifying primordial germ cells from the gonadof a 7-day-old chick embryo, and amplifying the separated primordial germ cells within 15 days by using a culture insert matched feeder layer method through an optimized culture system to obtain morethan one million cells so as to achieve rapid amplification and line establishment. According to the present invention, the method has characteristics of high success rate, short time, stability andhigh efficiency, can obtain more than 10<6> cells through the amplifying within in 15 d, and can establish the foundation for the poultry germplasm source conservation and preparation of transgenic chicken.

The invention belongs to the field of biological engineering and provides a method for separating human spermatogonial stem cells. The method comprises the following steps of: mechanically separating a testicular tissue; processing the testicular tissue into a semi-liquid state; adding digestive enzyme solution I into the liquid testicular tissue in the semi-liquid state; digesting the testiculartissue into a single convoluted tubule; adding digestive enzyme solution II in the convoluted tubule; digesting the convoluted tubule into a single cell; separating reproductive cells from supportingcells in the single cell; separating G protein-coupled receptor 125 (GPR125) positive spermatogonial stem cells from glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 1 (GFRA1)positive spermatogonial stem cells by adopting an immunomagnetic bead cell sorting method; and identifying the separated GPR125 positive spermatogonial stem cells and the GFRA1 positive spermatogonial stem cells by adopting an immune cell chemical method. By the method, a large number of the high-purity human spermatogonial stem cells are obtained, and sufficient cell sources are provided for theresearch of the human spermatogonial stem cells and the application of the human spermatogonial stem cells in regenerative medicine and reproductive medicine.

The invention provides a testicle cryopreservation agent for marine fishes and a preparation method of spermatogonium. The preparation method of spermatogonium comprises the following main steps: preparing a cryopreservation agent formula suitable for sciaenops ocellatus; establishing a testicle cryopreservation and unfreezing method; preparing the transplantable spermatogonium. The invention establishes a cryopreservation method for the spermatogonium of the sciaenops ocellatus and technical guarantees are provided for a reproductive cell transplanting technology. According to the testicle cryopreservation agent for the marine fishes and the preparation method of the spermatogonium, a donor source for transplanting reproductive cells is expanded; a testicle is cryopreserved for a long period and can be used according to requirements; guarantees are provided for the donor source for transplanting the reproductive cells and an application range of transplanting of the reproductive cells of fishes is greatly widened.

The invention relates to a magnetic nano-gene vector for transporting a gene, and a preparation technology and application thereof. The invention also provides a method for transforming animal and plant cells by a foreign gene loaded by the magnetic nano-gene vector. In the method, the magnetic nano-gene vector is nano Fe3O4 / PEI particles, and enters the animal and plant cells and / or generative cell with the foreign gene under drive of an external magnetic field; genetic transformation and expression are realized; and a new variety with excellent performance can be obtained through transgenosis cultivation. The preparation method and the transformation method disclosed by the invention are simple to operate, easy to control, and convenient to popularize. The transformation method disclosed by the invention is not limited by species; the receptor cell specificity is not required; and the transformation method can be widely applied to a transgenic technology of animals and plants. In the transformation method disclosed by the invention, the transformation efficiency of the gene can be significantly improved; and the copy number of the transferred foreign gene can be effectively controlled by controlling the quantity of the foreign genes combined with the magnetic nano-gene vector.

The transplant fish production method includes selecting a donor fish species, selecting first and second fish species which are a combination that could be sterile, producing a hybrid fish species of the selected first fish species and second fish species, and transplanting reproductive cells of the donor fish species into the produced hybrid fish species.

The invention discloses a slide climbing method of PGCs (primordial germ cells) and belongs to the biotechnology field. The slide climbing method comprises the steps as follows: purifying the PGCs; washing the purified PGCs with a PBS (phosphate buffer solution) for three times, and centrifugally collecting the cells; selecting and putting 100-200 cells in PBS droplets on a glass slide under a microscope for washing for one time; selecting and putting the cells in a paraformaldehyde solution with the concentration of 4% on the glass slide for washing for one time; selecting and putting the cells on paraformaldehyde droplets with the concentration of 4% on the glass slide for fixation for 10-30 min; and removing the paraformaldehyde with the concentration of 4% through adsorption, and finishing slide climbing of the cells while fixing the cells. According to the slide climbing method, the problem that the purified PGCs are difficult to climb the glass slide is solved, and the time and the cost are saved.

Compositions or extracts (2)(15)(24)(25)(16)(23)(31)(36)(41) obtained from the fruit (4) or leaves (26) of plants of the genus Hippophea (1) and methods of using such compositions or extracts to reduce loss of reproductive cell function.

The invention discloses a method to reprogram fibroblasts into Sertoli cells in vitro and application of the method. The method comprises the steps of introducing into fibroblasts, a fluorescent protein reporter system that specifically expresses in Sertoli cells; introducing a substance that increases NR5A1 protein expression and / or activity; adding G418, and culturing for 3-7 days; discarding anaqueous phase, adding a medium to culture fibroblasts, and continuing to culture for 3-7 days; isolating Sertoli cells from the culture system. Experiments prove that the cells prepared by the abovemethod fully attain the characteristics of Sertoli cells, such as ability to attract endothelial cells, ability to form lipid droplets, ability to interact with reproductive cells, ability to inhibitthe growth of lymphocytes and occurrence of interleukin, and ability of immune privilege and the like. The method has important applicable value in the fields, such as treatment of infertility, allograft, and cell therapy.

Provided are a vehicle body assembling method and a vehicle body assembling apparatus which allow a simple configuration in the vicinity of the connecting portion between an upper jig and a lower jig and allow an increase in the efficiency of assembling work (welding work). A vehicle body assembling apparatus is equipped with a jig for supporting vehicle body components in a preassembled position, the jig comprising an upper jig and a lower jig which are connected to each other in at least two places. Each of the connection places is provided with a connecting means for fixing a three-dimensional coordinate position while allowing uniaxial turning. The vehicle body assembling apparatus is also equipped with a conveying means for conveying the upper jig which supports the vehicle body components, and reduces the load applied to the lower jig from the upper jig when connecting the upper jig to the lower jig.

The present invention provides a liquid for cryopreservation of bovine reproductive cells such as bovine sperms and a cryopreservation method therefor. Use is made of a cryopreservation liquid wherein0.3-0.9 w / w% of an amphoteric polyelectrolyte (an antifreeze polyamino acid), said amphoteric polyelectrolyte being prepared by, relative to 50-99 mol% of amino groups in [epsilon]-poly-L-lysine having a number-average molecular weight of 1,000-20,000, reacting succinic anhydride and thus introducing an appropriate amount of carboxyl groups thereinto, and 2-4 w / w% of glycerol are dissolved in a physiological solution. A preferred embodiment of the cryopreservation method comprises: a first dilution step for diluting bovine semen 2.5-10 fold with a physiological solution and maintaining at 2-8DEG C; and a second dilution step for adding dropwise a physiological solution containing the amphoteric polyelectrolyte (antifreeze polyamino acid) and glycerol to the suspension obtained in the first dilution step while maintaining at 2-8 DEG C so as to further dilute the bovine semen 5-20 fold.

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer, and cryopreservation.

The invention belongs to the technical field of cell culture, particularly discloses an automatic cell culture device capable of simulating internal environments of organisms. The automatic cell culture device comprises a laminar flow sterile sealed chamber. An internal environment simulation tissue and organ culture device and a target cell induction culture device are arranged in the sealed chamber. The automatic cell culture device can simulate the internal environments including a low-oxygen low-permeability environment for culturing embryonic stem cells, a testicular internal environment during maturation of germ cells, a thymus environment for inducing maturation of T cells, a cerebrospinal fluid environment for inducing differentiation of neural stem cells and tissue and organ environments such as livers, pancreas, bone marrow and tumors for inducing differentiation and maturation of corresponding stem cells, and cultures specific high-quality cells in an induction mode to meet needs of cell basic researches and applications. Besides, the simulated internal environments provide a basis for researching an organism cell growth, differentiation and maturation mechanisms. Therefore, the improvement of cell culture quality is facilitated, repeatability of cytology researches is remarkably increased, cost of cell culture is saved, clinic transformation and application of the cell culture technology are quickened, and a good experiment platform is provided for scientific research basic workers.

The invention mainly relates to the field of aquaculture technologies, and discloses a method for artificially breeding parabramis pekinensis. The method includes selecting parent fish; cultivating the parent fish; artificially inducing spawning; carrying out artificial insemination; carrying out artificial hatching. The method has the advantages that the method is simple, the reproduction periods of the parabramis pekinensis can be brought forward by 12-15 days, the reproductive capacity of the parabramis pekinensis can be improved and can reach to 8021 roes, and economic income can be increased by 17.3%; the parent fish of the parabramis pekinensis is cultivated in indoor environments, special parent fish feeding agents are splashed, growth conditions required by the female and male parent fish can be regulated, water can be purified, gonad development can be promoted, the disease resistance of the parent fish and the viability of reproductive cells can be enhanced, the fertilization rate can be increased and is 92%, debonding is carried out after fertilization is carried out, debonded liquid is extracted and then is fermented by lactobacillus acidophilus, has appropriate pH (potential of hydrogen), is antibacterial and insecticidal, contains abundant nutrition, is safe and healthful and is debonded quickly, the viability of cells of fertilized roes can be enhanced, and the hatching rate can reach to 91%.

Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian or avian reproductive sperm cells. The compositions comprise suitable reproductive cell media and antioxidant bioflavonoids. These may be comprised of Oligomeric Proanthocyanidins (OPC) which include the specific molecules of OPCs, that are namely; (epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGCG)).

The invention relates to external physical therapy liquid for treating thyrophyma. The external physical therapy liquid comprises the following raw materials in parts by weight: 25-35 parts of ternate buttercup root, 15-20 parts of stiff silkworm, 10-15 parts of semen brassicae, 8-10 parts of gamboge, 5-10 parts of ephedra, 8-10 parts of rhizoma polygonati, 20-24 parts of thunberg fritillary bulb, 10-13 parts of kelp, 15-25 parts of platycodon grandiflorum, 8-10 parts of selfheal, 12-18 parts of glossy privet fruit, 10-15 parts of caulis spatholobi, 5-7 parts of eclipta alba, 8-15 parts of sculellaria barbata and 12-18 parts of white paeony root. The several traditional Chinese medicines in compatibility have obvious curative effects of thyrophyma, and the effective rate is 92%. Meanwhile, the traditional Chinese medicines have obvious curative effects for treating scrofula and goiter. The external physical therapy liquid disclosed by the invention has effects of inhibiting tumor cell growth, proliferation and metastasis, killing cancer cells, repairing damaged normal cells and new reproductive cells, easing pain, relieving depression, obviously relieving pain, improving unhealthy emotions and alleviating uncomfortable symptoms of the whole body and also has the effects of promoting appetite, improving sleep, balancing nutrition and invigorating health effectively.

The invention relates to an anti-seasonal reproduction method of crucian carp. The reproduction of crucian carp in winter includes the following steps: (1) disinfecting a temporary culture pond and ahatching tool; (2) preparing a parent fish culture pond; (3) parent fish and breeding thereof; (4) putting parent fish in the pond for temporary culture according to a male-to-female ratio and corresponding parent fish screening criteria; (5) spawning induction; and (6) fertilization and hatching. The reproduction of crucian carp in summer includes the following steps: (1) disinfecting a temporaryculture pond and a hatching tool; (2) parent fish and breeding thereof; (3) putting parent fish in the pond according to a male-to-female ratio; (4) spawning induction; and (5) fertilization and hatching. Different reproduction methods are set for different seasons, the water temperature is adjusted reasonably, and the proportion of oxytocic is set creatively. Thus, the parent fish are in a physiological state of normal ovulation or spermiation, and the vitality of reproductive cells is improved. Through the innovation of the breeding mode and the screening criteria, the problem of egg agingof female fish is solved.

The invention discloses a 3D culture method of chicken embryo primordial germ cells. The 3D culture method comprises the following steps: 1), placing collected 14HH chicken embryo blood in a cPGCs complete medium for culturing, and purifying cells; 2), placing the purified chicken embryo primordial germ cells into the cPGCs complete medium again for in-vitro culture amplification, taking subcultured second-generation cells, and centrifuging to obtain a chicken embryo primordial germ cell precipitate; 3), adding a sodium alginate solution to the precipitate; 4), sucking a cell suspension by using a short-needle insulin syringe, dropwise adding into a culture dish containing a CaCl2 solution to form sodium alginate hydrogel spheres, standing for 15min and then culturing. Through introductionof sodium alginate into a culture condition, the in-vivo growth environment of the chicken embryo primordial germ cells can be simulated very well in a 3D cell culture mode, so that obtainment of a large number of chicken embryo primordial germ cells is facilitated, and thus application of the chicken embryo primordial germ cells to research and production is facilitated.