4 results about "Reproductive cells" patented technology

The application belongs to the technical field of medicine, and discloses application of uridine-5' monophosphate and a compound thereof in preparation of a medicine for enhancing the proliferation ability of male reproductive cells. It is found for the first time that the uridine-5' monophosphate has the proliferation ability on the male reproductive cells, and the proliferation effect is best when the concentration of the uridine-5' monophosphate is 10 μM, the proliferation effect on the mouse TM3 cells is increased by 52.87% compared with a control, and the proliferation effect on the mouse TM4 cells is increased by 53.57%. In addition, the uridine-5' monophosphate has a synergistic ability when being compounded with xylonic acid lactone, and further improves the proliferation effect on the TM3 cells, thereby laying a foundation for treatment of male reproductive system diseases in the future.

The invention discloses a low-temperature preservation and resuscitation device for reproductive medicine, and belongs to the technical field of low-temperature preservation and resuscitation. The resuscitation assembly is connected with the top of the base so as to resuscitate the sample test tube filled with the germ cells; the low-temperature preservation assembly is detachably arranged at the top of the resuscitation assembly and is communicated with the resuscitation assembly so as to perform low-temperature preservation on the sample test tube filled with the germ cells; wherein a switch assembly is arranged at the communicating position of the low-temperature preservation assembly and the recovery assembly, and opening and closing of the communicating position are achieved; and the driving assembly is connected to the low-temperature preservation assembly, so that the sample test tubes placed on the driving assembly are fed into the resuscitation assembly. Through integrated and automatic design, seamless transfer of the sample between preservation and recovery is realized, temperature fluctuation and pollution risks are reduced, the operation efficiency, the standardization degree and the cell survival rate are improved, and meanwhile, the cost is reduced.

This invention discloses an antibody for prolonging ovarian function, a culture medium, a method for inhibiting primordial follicle activation, and its application. The antibody is a CD138 antibody. In the technical solution provided by this invention, the CD138 antibody can precisely regulate the transition rhythm of primordial follicles from a quiescent state to a developing state by targeting and inhibiting the primordial follicle activation signaling pathway. This regulatory mechanism can reduce the ineffective activation and loss of follicles in early reproductive years, allowing more primordial follicles to remain dormant, thereby prolonging the physiological lifespan of the follicle pool. This provides biological protection for the even distribution of reproductive cell resources throughout the reproductive cycle in female individuals, effectively avoiding the risk of infertility or premature ovarian failure caused by premature follicle depletion.

The application discloses a RNAi interference fragment targeting a donkey Zfy gene, a carrier and a preparation method and application thereof, and relates to the technical field of animal molecular genetics, wherein the RNAi interference fragment comprises RNAi-3-F and RNAi-3-R, and the nucleotide sequences are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The RNAi interference fragment provided by the application can target and silence Y chromosome sex determination related genes in male donkey Y spermatogenesis stage key reproductive cells, hinder and interfere with the normal development and physiological functions of Y sperm, regulate the key genes of Y sperm development through specific silencing, realize sex regulation before fertilization, greatly improve the proportion of female individuals of offspring donkeys, and the scheme does not damage target sperm cells and does not reduce sperm activity, and meanwhile, the scheme has the advantages of simple operation and low production cost.