42 results about "Y chromosome" patented technology

The invention relates to a method for efficiently producing YY transformed females of Nile tilapia with a high reproductive rate. Firstly, the XY transformed females are mated with XY males or YY super males, and the offspring are simultaneously fertilized 5 days after fertilization. Soak in estrogen and androgen synthase inhibitors until 10 days after insemination; then feed high-dose hormone-supplemented feed for feminization for 3 months after juveniles open, and use Y chromosome-specific molecular markers to screen out sex chromosomes The transformed female fish with genotype YY were fed with normal feed until 6 months old; then at the age of 6-7 months, the YY transformed female fish were treated with low-dose hormone-supplemented feed, and were raised normally until sexual maturity; finally, the fertile YY transformed female fish were screened out . Fertile YY transformed females are mated with YY super males to establish YY maintenance lines and realize large-scale production of genetic all-male fry, which can significantly increase aquaculture production, reduce aquaculture costs, and reduce the use of hormones. In areas where tilapias invade and flood A large number of YY super male fish can be released for biological control.

The invention relates to an amplification composition for detecting abnormal number of chromosomal aneuploid and a rapid detection kit, and belongs to the technical field of biology. The amplification composition can be used for amplification detection of STR sites related to 8 21# chromosomes, 6 18# chromosomes, 6 13# chromosomes and 3 X chromosomes and amplification detection of four amelo; all sites can be amplified by a quantitative fluorescent PCR (QF-PCR) technology, so as to detect the abnormal number of 21, 18, 13, X and Y chromosomal aneuploid. According to the kit, detection cycle is short, so the result can be obtained within 5 hours after obtaining the sample; the sensitivity is high; DNA at nanogram level, which is equal to DNA contained in 100 cells, is needed for each detection, so few samples are needed; 1 to 2ml of amniotic fluid can be collected to meet the demand; due to high sensitivity, a chimera containing more than 10% of trisomies can be detected.

The present invention provides methods for non-invasive determination of sex in a fetus or of Y chromosomal frequency abnormalities—indicative of aneuploidy or sex mosaicisms in a fetus—by detecting and determining the relative contribution genetic sequences from the Y chromosome in view of the percent fetal contribution in a maternal mixed sample.