8 results about "Y chromosome" patented technology

The invention discloses a dairy cow early embryo sex identification method, and belongs to the technical field of dairy cow breeding and breeding. The identification method comprises the following steps: (1) culturing a dairy cow embryo to an early blastocyst stage, starting to perform single-embryo single-droplet culture, and after culturing until a blastocyst cavity is formed, punching a blastocyst zona pellucida to promote the blastocyst to shrink and release blastocyst cavity liquid; (2) collecting blastocyst cavity liquid, and extracting embryo free DNA in the blastocyst cavity liquid; (3) by taking the free DNA as a template, designing a primer to amplify a specific repetitive sequence ChrY fragment of the bull Y chromosome; if the amplification product exists, the embryo is identified as a bull, and if the amplification product does not exist, the embryo is identified as a cow. The method can ensure that the embryo free DNA in the obtained blastocyst cavity fluid is only derived from the embryo, and can effectively avoid embryo injury. According to the method disclosed by the invention, the copy number of the amplified target area in the bull Y chromosome is more than 2000 times, a detection signal can be amplified, and the detection accuracy is improved.

A sex chromosome typing method and apparatus, a device, a medium, and a product. The method comprises: first, on the basis of a Y chromosome concentration of a nucleic acid sample to be tested in a cfDNA sample to be tested, determining whether the nucleic acid sample to be tested carries a Y chromosome; in a branch of not carrying a Y chromosome, on the basis of an X chromosome concentration and an X chromosome concentration range, determining a sex chromosome type of the nucleic acid sample to be tested; and in a branch of carrying a Y chromosome, using the ratio of the X chromosome concentration to the Y chromosome concentration as a concentration ratio to be tested, and, on the basis of the concentration ratio to be tested and Cauchy distribution functions respectively corresponding to at least two preset sex chromosome types, determining the sex chromosome type of the nucleic acid sample to be tested, thereby solving the problem that conventional typing methods rely on a sample set, and reducing testing costs and maintenance costs while ensuring the accuracy of sex chromosome typing.

The invention belongs to the technical field of molecular biology. More specifically, the invention relates to a specific sequence of a humulus lupulus Y chromosome and application of the specific sequence in sex determination. The invention provides a humulus lupulus Y chromosome specific sequence and a matched primer pair thereof. The humulus lupulus Y chromosome specific sequence and the matched primer pair can be used for carrying out accurate, efficient and stable molecular identification on the sex of humulus lupulus in a seedling stage. The primer pair Y1F / Y1R designed by aiming at the sequence shows highly consistent amplification effects in a plurality of cultivated varieties and wild materials from different ecological regions, all male samples can be stably amplified to form clear bands, and female samples do not have any non-specific amplification, so that the judgment accuracy rate reaches 100%. In conclusion, the invention provides a simple, convenient, rapid and stable technical means for sex determination of humulus lupulus, and has important application value in the aspects of breeding population construction, cross parent screening, fundamental research and the like.

This invention belongs to the field of plant sex identification technology, and particularly relates to the application of a molecular marker in identifying the sex of *Zephyranthes* plants. The molecular marker is linked to the Y chromosome of *Zephyranthes* plants and comprises a sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with SEQ ID NO:1. The molecular marker provided by this invention can be tightly linked to the Y chromosome of *Zephyranthes* plants, thereby achieving the purpose of early and rapid sex detection. This has significant practical implications for sex selection and proportioning of ornamental garden plants, understanding and protecting endangered *Zephyranthes* germplasm resources, and improving the effectiveness of ex-situ and in-situ conservation management.

The invention discloses a Pelteobagrus vachelli sex identification molecular marker, a development method and application, the Pelteobagrus vachelli sex identification molecular marker is located on a No.3 chromosome, the Pelteobagrus vachelli sex identification molecular marker is a deletion specific marker having a segment of 52bp compared with a Pelteobagrus vachelli X chromosome, and the nucleotide sequence of the deletion specific marker is as shown in SEQ ID NO: 1. By constructing a full-sib family group with a clear genetic background and combining grading comparison and genomics analysis, a stable and reliable pelteobagrus vachelli sex specificity marker is screened out, rapid and accurate sex identification in the fry stage is achieved, the method has great significance in reducing the breed conservation cost and improving the breed production efficiency, and the method is suitable for popularization and application of the pelteobagrus vachelli sex specificity marker and the pelteobagrus vachelli sex specificity marker. And key technical support is provided for sustainable development of the pelteobagrus fulvidraco breeding industry.

The present invention relates to an antibody or antigen-binding fragment thereof that specifically binds to porcine Y sperm, a composition comprising same, and use thereof. It was confirmed that treatment with the antibody or an antigen-binding fragment thereof, of the present invention, induced an aggregation reaction of Y chromosome sperm, thereby enabling easy distinction between X chromosome sperm and the Y chromosome sperm. Thus, pigs having a specific sex can be mass-produced, and pigs having a desired sex can be selectively produced, thereby contributing to planned breeding, breeding improvement, and management efficiency.

This application discloses a method, device, equipment, and medium for sex chromosome detection based on a vector space projection model, relating to the fields of bioinformatics and gene detection. The method includes: aligning high-throughput sequencing data of the sample to be tested to a reference genome for copy number analysis to obtain the overall copy number of the X and Y chromosomes; if there is an abnormality in the overall copy number of the X / Y chromosome, mapping the sample to be tested to a position point in a two-dimensional vector space based on the overall copy number of the X and Y chromosomes, and calculating the perpendicular distances from the sample to the multiple reference evolution vectors in the two-dimensional vector space, taking the sex chromosome variation path represented by the reference evolution vector with the smallest distance as the sex chromosome variation path of the sample to be tested; comparing and analyzing the overall copy number of the X / Y chromosome with the judgment threshold corresponding to the sex chromosome variation path, and outputting the sex chromosome variation detection result.

The application discloses a RNAi interference fragment targeting a donkey Zfy gene, a carrier and a preparation method and application thereof, and relates to the technical field of animal molecular genetics, wherein the RNAi interference fragment comprises RNAi-3-F and RNAi-3-R, and the nucleotide sequences are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The RNAi interference fragment provided by the application can target and silence Y chromosome sex determination related genes in male donkey Y spermatogenesis stage key reproductive cells, hinder and interfere with the normal development and physiological functions of Y sperm, regulate the key genes of Y sperm development through specific silencing, realize sex regulation before fertilization, greatly improve the proportion of female individuals of offspring donkeys, and the scheme does not damage target sperm cells and does not reduce sperm activity, and meanwhile, the scheme has the advantages of simple operation and low production cost.