37 results about "Y chromosome" patented technology

The invention belongs to the medical detection field, and discloses a method and a system for noninvasive detection of fetus chromosome aneuploid. The disclosed detection method and system also relate to a method and a system for elimination of sequencing GC preference in chromosomes and among chromosomes and a method and a system used for the relation model of the Z values of X and Y chromosomes in a normal male fetus. Through elimination of influences of sequencing GC preference in chromosomes and among chromosomes, the relation model of the Z values of X and Y chromosomes in a normal male fetus is built, and the determination threshold of difference between the theoretical value and the actual value of the Z value of the X chromosome is built. The accurate detection of fetus chromosome aneuploid, especially sex chromosome aneuploid is achieved.

The invention relates to an amplification composition for detecting abnormal number of chromosomal aneuploid and a rapid detection kit, and belongs to the technical field of biology. The amplification composition can be used for amplification detection of STR sites related to 8 21# chromosomes, 6 18# chromosomes, 6 13# chromosomes and 3 X chromosomes and amplification detection of four amelo; all sites can be amplified by a quantitative fluorescent PCR (QF-PCR) technology, so as to detect the abnormal number of 21, 18, 13, X and Y chromosomal aneuploid. According to the kit, detection cycle is short, so the result can be obtained within 5 hours after obtaining the sample; the sensitivity is high; DNA at nanogram level, which is equal to DNA contained in 100 cells, is needed for each detection, so few samples are needed; 1 to 2ml of amniotic fluid can be collected to meet the demand; due to high sensitivity, a chimera containing more than 10% of trisomies can be detected.

A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXa for the X chromosome, AluSTYa for the Y chromosome, or both AluSTXa and AluSTYa, to reduce the possibility of error to a negligible quantity. The inserted chromosome yields a large fragment when the homologous region is amplified. The males are distinguished as having two DNA amplicons present, while females have only a single amplicon. The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction regents.

The invention provides a primer for detecting the X and Y sperm separation purity. Aiming at a sex-determining gene Sry on a bull Y chromosome, the primer is designed through the PCR mismatching technology. The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result. The invention provides the technology used for identifying the bull X and Y sperm separation purity with low cost and simple, rapid, and accurate operation, and provides reliable technical support for the popularization and the application of the subsequent sexing semen and the optimization of a sperm separation method.

Technologies for assessing, quantifying and isolating sperm cell populations and/or subpopulations having specific genetic signatures are provided, as well as methods and systems to assess the efficacy of chromosomal differentiation processes. Compositions for identification and differentiation of X-chromosomes and Y-chromosomes in DNA are also provided.

The present invention relates to a method for detecting abnormal numbers of five chromosomes, wherein the method is provided for concurrently detecting aneuploidy of numbers of five chromosomes such as chromosome 21, chromosome 13, chromosome 18, chromosome X and chromosome Y, and comprises: (1) respectively selecting 7 genes from the five chromosomes, and designing 38 pairs of primers based on the selected genes; (2) adopting combinations of the primers to carry out multiplex PCR and capillary electrophoresis; and (3) adopting a software to analyze electrophoresis results.

The present invention relates to DNA sequences, probes and primers specific to the Y chromosome of Equus caballus. The present invention also relates to methods for determining the sex of horses, fetal horses, horse embryos or horse cells. The present invention further relates to methods for isolating Y chromosome DNA sequences. .

The invention discloses a multiple relative real-time fluorescent quantitative PCR detection kit for rapidly detecting the number of human chromosomes. The kit comprises two reaction tubes of a group A reaction system and a group B reaction system, wherein the group A reaction system comprises two pairs of primers and four Taqman probes which are used for detecting repetitive sequences between chromosomes 21 and 18 and repetitive sequences between chromosomes 13 and 1, and are mainly used for detecting the number of chromosomes 13, 18 and 21; the group B reaction system comprises two pairs of primers and four Taqman probes which are used for detecting repetitive sequences between chromosomes 16 and X and repetitive sequences between chromosomes 1 and Y, and are mainly used for detecting the number of chromosomes X and Y. The kit disclosed by the invention is capable of respectively detecting the number of the chromosomes 13, 18, 21, X and Y simultaneously and independently; the results are accurate and reliable; the kit has high sensitivity and specificity.

Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.

The invention provides a multi-PCR amplification system and detection kit for human Y-chromosomal microdeletion. The system comprises amplification primers comprising following loci with the corresponding sequences from SEQ ID No.1 to SEQ ID No.40, wherein the loci comprise 15 Y-chromosomal microdeletion loci (4 in the AZFa region, 4 in the AZFb region, 5 in the AZFc region and 2 in AZFd region),3 sample molecular tag loci (two X-STR loci and one autosomal locus) and 2 gender quality control loci (SRY, ZFX/Y). The 15 Y-chromosomal microdeletion loci use sequence tag loci (STS) specific primers, the 3 sample molecular tag loci use length polymorphism primers, and the 2 gender quality control loci use STS specific primers (EAA/EMQN Y-chromosomal microdeletion related aboratory optimal handbook recommendation detection loci, 2013). The primers are compatible with one another and can react in the same PCR amplification system. In addition, the invention further provides the detection kitcontaining an amplification primer composition container, a PCR reaction mother liquor container and a PCR auxiliary liquid container and application of the detection kit in human Y-chromosomal microdeletion non-diagnostic detection. After DNA extraction, target fragments of 20 genetic marker loci are amplified through a PCR reaction, the cost, labor and time can be remarkably saved, and the workefficiency is improved.

Methods and compositions are provided for generating F0 fertile XY female animals. The methods and compositions involve making XY pluripotent or totipotent animal cells, in vitro cell cultures, or embryos that are capable of producing a fertile female XY animal in an F0 generation. Such cells, embryos, and animals can be made by silencing a region of the Y chromosome. Optionally, the cells can also be cultured in feminizing medium such as a low-osmolality medium and/or can be modified to decrease the level and/or activity of a Sry protein. Methods and compositions are also provided for silencing a region of the Y chromosome in an XY pluripotent or totipotent animal cell, or in vitro cell cultures, embryos, or animals derived therefrom, by maintaining an XY pluripotent or totipotent animalcell in a feminizing medium. Methods and compositions are also provided for maintaining a population of XY pluripotent or totipotent animal cells in a feminizing medium and selecting cells or clones having increased capabilities for producing a fertile female XY animal in an F0 generation. Methods and compositions are also provided for screening for compounds with feminizing activity or for optimizing concentrations of components in feminizing media.

The invention discloses a multiplex amplification kit for cattle paternity identification and individual identification and an application thereof. The kit comprises 21 pairs of specific primers for amplifying 20 cattle STR sites and one Amel sex site, the sequences of the specific primers are shown as SEQ ID NO: 1-SEQ ID NO: 42 in a sequence table, and 20 cattle STR sites and one Amel sex site can be amplified at the same time. The invention provides the multiplex amplification kit for cattle paternity identification and individual identification and the application thereof. The kit comprisesan autosomal STR, a Y chromosome STR and an Amel sex site at the same time, the kit is the kit with the most detection sites in the same type of products, can simultaneously amplify and detect 20 cattle STR sites and one cattle sex site in a single tube, has the characteristics of strong specificity, high resolution, accurate typing, high sensitivity and the like, and can completely meet the requirements of cattle paternity identification, individual identification, sex discrimination and the like.

The invention discloses a Y-chromosome modification method and an application thereof. The invention discloses an animal Y-chromosome modification method which comprises performing specific suicide component modification on the Y-chromosome of an isolated animal body cell by use of a TALEN method. The Y-chromosome modification method lays a foundation for selectively breeding animals of specific gender and improving the breeding efficiency.

The invention relates to the technical and medical fields of molecular biology genes, in particular to a Y-chromosomal microdeletion detection method and primer group. The nucleotide sequence of sevensequence tag sites of AZFasY84, AZFasY86, AZFasY127, AZFasY134, AZFasY254, AZFasY255 and SRYsY14 is analyzed, corresponding primers are designed, multiple PCR (polymerase chain reaction) amplification on specific sites of genomic DNA in the sample is performed, and finally detection is carried out with the Ion Torrent semiconductor chip sequencing technology. The detection method has the advantages of large flux, high specificity and sensitivity, stable results, good repeatability, fast detection speed and the like; a fast, reliable and accurate new approach is provided for detecting, testing, analyzing and evaluating the genetic aspects of male infertility, and theoretical basis is provided for clinical diagnosis of the diseases.

The invention discloses a reagent and a kit for detecting sex-associated hereditary diseases of fetuses. The reagent for detecting the sex-associated hereditary disease of the fetus comprises a Y chromosome specific primer and a Y chromosome specific probe, an upstream primer of the Y chromosome specific primer is Seq ID No.1, a downstream primer of the Y chromosome specific primer is a sequence shown as Seq ID No.2, and the Y chromosome specific probe is a probe with a fluorescent group at the 5'end and a quenching group at the 3 'end of a sequence shown as Seq ID No.3. According to the reagent for detecting the sex-associated hereditary disease of the fetus, the sex of the fetus can be judged by detecting blood of a pregnant woman, and the sex-associated hereditary disease is detected; the sex-associated hereditary disease related to the gender of the fetus can be found as soon as possible in the early pregnancy stage, and reference is provided for related treatment.

The invention discloses a molecular marker for identifying genetic sex of micropterus salmoides and a primer pair thereof. The sequence of the molecular marker is shown as SEQ ID NO: 1, and the molecular marker exists on a genetic sex X chromosome of micropterus salmoides and is deleted on a Y chromosome. The sequences of the primer pair are shown as SEQ ID NO: 2 and SEQ ID NO: 3. The primer pair can be used for simply, quickly and stably identifying the genetic sex of different individuals in each population of the micropterus salmoides, including embryos, larvae and adults of the micropterus salmoides. And the accuracy reaches 100%.

The invention discloses a sex identification method of pike vibrio molitor, and claims to protect a specific molecular marker of genetic sex of the pike vibrio molitor, the specific molecular marker has difference between X chromosome and Y chromosome of the pike vibrio molitor, and the difference can be used for identifying the genetic sex of the pike vibrio molitor. The invention overcomes the defects in the prior art, provides a specific molecular marker based on gender linkage, can simply and quickly realize genetic gender, and greatly saves manpower and material resources.

The invention relates to a mouse in-vitro fertilization breeding sex selection method, which comprises the following steps: inserting a luciferase gene into a Y chromosome of a male mouse by utilizing a gene editing technology, and carrying out embryo injection and transplantation to obtain a Lu-Y male mouse through gene identification; adding a substrate of luciferase into a sperm preserving fluid of a Lucy-Y male mouse, where Lucy-Y sperms emit fluorescence with the wavelength of 560 nm, and normal X sperms do not have fluorescence; sorting lucc-Y sperms and normal X sperms through a flow cytometry sorter; observing the sorted luc-Y sperms and normal X sperms by using a fluorescence microscope; judging the proportion of Luc-Y sperms in normal X sperms through fluorescence; and if the proportion exceeds the standard, putting the normal X sperms into the flow cytometry sorter again for sorting until the purity of the normal X sperms reaches the standard. According to the invention, the flow sorting result can be conveniently and intuitively judged through naked eyes, and the screening precision of the X sperms and the Y sperms of the mice is effectively improved.