14 results about "Y chromosome" patented technology

The invention discloses a dairy cow early embryo sex identification method, and belongs to the technical field of dairy cow breeding and breeding. The identification method comprises the following steps: (1) culturing a dairy cow embryo to an early blastocyst stage, starting to perform single-embryo single-droplet culture, and after culturing until a blastocyst cavity is formed, punching a blastocyst zona pellucida to promote the blastocyst to shrink and release blastocyst cavity liquid; (2) collecting blastocyst cavity liquid, and extracting embryo free DNA in the blastocyst cavity liquid; (3) by taking the free DNA as a template, designing a primer to amplify a specific repetitive sequence ChrY fragment of the bull Y chromosome; if the amplification product exists, the embryo is identified as a bull, and if the amplification product does not exist, the embryo is identified as a cow. The method can ensure that the embryo free DNA in the obtained blastocyst cavity fluid is only derived from the embryo, and can effectively avoid embryo injury. According to the method disclosed by the invention, the copy number of the amplified target area in the bull Y chromosome is more than 2000 times, a detection signal can be amplified, and the detection accuracy is improved.

The invention provides a sex-linked molecular marker-assisted all-male silurus asotus breeding method, which belongs to the technical field of silurus asotus sex breeding, and comprises the following steps: step 1, screening a molecular marker SSR-Y1 linked with silurus asotus Y chromosome, and the gene base sequence of the molecular marker SSR-Y1 is as shown in SEQ ID NO: 1; 2, designing a specific primer pair SSR-Y1: F: 5 '-ATGTCGATCCGAGACCT-3' and a specific primer pair SSR-Y1: R: 5 '-TGCAGATCGTCGATAGG-3', and carrying out amplification on the specific primer pair SSR-Y1: F: 5 '-ATGTCGATCGAGACCT-3'; 3, extracting the genome DNA of an individual to be detected, and amplifying the SSR-Y1 marker through PCR (Polymerase Chain Reaction); 4, judging the gender according to an amplification product, wherein male individuals show 350bp bands, and female individuals do not have bands; and 5, selecting a male parent with the genetic sex of XY, and hybridizing the male parent with the common female XX to obtain an all-male offspring XY. According to the invention, the SSR-Y1 marker is adopted, and the SSR-Y1 marker has the advantages of strong specificity, high accuracy, no consumption of a large amount of manpower and low cost; the genetic sex of the catfish is rapidly identified through PCR amplification, the production process of unisexual fish fries of the catfish is simplified by means of a molecular marker-assisted breeding technology, the breeding speed is increased, and the cost is saved.

This invention provides a method for identifying catfish ( Silurus asotus This invention discloses a specific molecular marker for sex identification. The molecular marker is a DNA fragment located on the Y chromosome of catfish, and its nucleotide sequence is shown in SEQ ID No. 3. A DNA fragment partially homologous to this fragment is located on chromosome 8, and its nucleotide sequence is shown in SEQ ID No. 4. This invention is the first to screen male-specific fragments from catfish genome information and design specific primers for genetic sex identification of catfish. The genetic sex PCR identification method based on this molecular marker is applicable to genetic sex identification and screening of catfish at different growth stages and in different populations in different watersheds in the field, in laboratories, or in aquaculture enterprises. Since female catfish grow faster than males, applying this method to assist in the production of all-female fry helps improve the efficiency of aquaculture.

A sex chromosome typing method and apparatus, a device, a medium, and a product. The method comprises: first, on the basis of a Y chromosome concentration of a nucleic acid sample to be tested in a cfDNA sample to be tested, determining whether the nucleic acid sample to be tested carries a Y chromosome; in a branch of not carrying a Y chromosome, on the basis of an X chromosome concentration and an X chromosome concentration range, determining a sex chromosome type of the nucleic acid sample to be tested; and in a branch of carrying a Y chromosome, using the ratio of the X chromosome concentration to the Y chromosome concentration as a concentration ratio to be tested, and, on the basis of the concentration ratio to be tested and Cauchy distribution functions respectively corresponding to at least two preset sex chromosome types, determining the sex chromosome type of the nucleic acid sample to be tested, thereby solving the problem that conventional typing methods rely on a sample set, and reducing testing costs and maintenance costs while ensuring the accuracy of sex chromosome typing.

The invention belongs to the technical field of molecular biology. More specifically, the invention relates to a specific sequence of a humulus lupulus Y chromosome and application of the specific sequence in sex determination. The invention provides a humulus lupulus Y chromosome specific sequence and a matched primer pair thereof. The humulus lupulus Y chromosome specific sequence and the matched primer pair can be used for carrying out accurate, efficient and stable molecular identification on the sex of humulus lupulus in a seedling stage. The primer pair Y1F / Y1R designed by aiming at the sequence shows highly consistent amplification effects in a plurality of cultivated varieties and wild materials from different ecological regions, all male samples can be stably amplified to form clear bands, and female samples do not have any non-specific amplification, so that the judgment accuracy rate reaches 100%. In conclusion, the invention provides a simple, convenient, rapid and stable technical means for sex determination of humulus lupulus, and has important application value in the aspects of breeding population construction, cross parent screening, fundamental research and the like.

The present application belongs to the technical field of molecular biology, and particularly relates to a gender identification molecular marker based on Y chromosome specific deletion of Micropterus salmoides, a primer pair and a gender identification method. The marker disclosed by the present application is a 147 bp deletion sequence (SEQ ID NO. 1) on the Y chromosome of male individuals, and has a complete genetic relationship with gender, wherein the XX genotype corresponds to female individuals, the XY genotype corresponds to male individuals, and the YY genotype corresponds to super-male individuals. Meanwhile, a primer pair for specifically amplifying the marker is provided. When detection is performed, only the genomic DNA of fin tissue of an individual to be detected needs to be extracted, PCR amplification is performed by using the primer pair, and the amplified bands are analyzed by agarose gel electrophoresis. Then, the genotype can be accurately determined according to the number of bands. The method has the remarkable advantages of simple operation, low cost and reliable results.

This invention belongs to the field of plant sex identification technology, and particularly relates to the application of a molecular marker in identifying the sex of *Zephyranthes* plants. The molecular marker is linked to the Y chromosome of *Zephyranthes* plants and comprises a sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with SEQ ID NO:1. The molecular marker provided by this invention can be tightly linked to the Y chromosome of *Zephyranthes* plants, thereby achieving the purpose of early and rapid sex detection. This has significant practical implications for sex selection and proportioning of ornamental garden plants, understanding and protecting endangered *Zephyranthes* germplasm resources, and improving the effectiveness of ex-situ and in-situ conservation management.

The invention belongs to the technical field of molecular biology, and particularly relates to a sex identification molecular marker based on micropterus salmoides Y chromosome specific deletion, a primer pair and a sex identification method. The marker disclosed by the invention is a 147bp deletion sequence (SEQ ID NO.1) on a Y chromosome of a male individual, and is in a complete linkage genetic relationship with sex, an XX genotype corresponds to a female individual, an XY genotype corresponds to a male individual, a YY genotype corresponds to a super-male individual, meanwhile, a primer pair for specifically amplifying the marker is provided, and the primer pair is used for detecting sex of the male individual. During detection, the genotype can be accurately judged according to the number of bands only by extracting the genome DNA of the fin ray tissue of an individual to be detected, performing PCR amplification by using the primer pair and analyzing amplified bands through agarose gel electrophoresis, and the method has the remarkable advantages of simplicity and convenience in operation, low cost and reliable result.

The application discloses a Trichiurus lepturus gender-specific molecular marker and a primer pair thereof. The application first excavates a DNA fragment with stable difference existing in the genomes of male and female Trichiurus lepturus, i.e., the sequence of the molecular marker is shown as SEQ ID NO:1, which exists on the genetic sex X chromosome of the Trichiurus lepturus and is absent on the Y chromosome. The sequences of the primer pair are shown as SEQ ID NO:2 and 3. A genetic sex identification method of the Trichiurus lepturus is established, and the genetic sex of the Trichiurus lepturus can be accurately identified, which lays a foundation for related genetic basic research. The application has the characteristics of wide application range, simple operation, high accuracy and the like, and has important application value in Trichiurus lepturus gender proportion monitoring and resource investigation.

The invention discloses a Pelteobagrus vachelli sex identification molecular marker, a development method and application, the Pelteobagrus vachelli sex identification molecular marker is located on a No.3 chromosome, the Pelteobagrus vachelli sex identification molecular marker is a deletion specific marker having a segment of 52bp compared with a Pelteobagrus vachelli X chromosome, and the nucleotide sequence of the deletion specific marker is as shown in SEQ ID NO: 1. By constructing a full-sib family group with a clear genetic background and combining grading comparison and genomics analysis, a stable and reliable pelteobagrus vachelli sex specificity marker is screened out, rapid and accurate sex identification in the fry stage is achieved, the method has great significance in reducing the breed conservation cost and improving the breed production efficiency, and the method is suitable for popularization and application of the pelteobagrus vachelli sex specificity marker and the pelteobagrus vachelli sex specificity marker. And key technical support is provided for sustainable development of the pelteobagrus fulvidraco breeding industry.

The present invention relates to an antibody or antigen-binding fragment thereof that specifically binds to porcine Y sperm, a composition comprising same, and use thereof. It was confirmed that treatment with the antibody or an antigen-binding fragment thereof, of the present invention, induced an aggregation reaction of Y chromosome sperm, thereby enabling easy distinction between X chromosome sperm and the Y chromosome sperm. Thus, pigs having a specific sex can be mass-produced, and pigs having a desired sex can be selectively produced, thereby contributing to planned breeding, breeding improvement, and management efficiency.

This application discloses a method, device, equipment, and medium for sex chromosome detection based on a vector space projection model, relating to the fields of bioinformatics and gene detection. The method includes: aligning high-throughput sequencing data of the sample to be tested to a reference genome for copy number analysis to obtain the overall copy number of the X and Y chromosomes; if there is an abnormality in the overall copy number of the X / Y chromosome, mapping the sample to be tested to a position point in a two-dimensional vector space based on the overall copy number of the X and Y chromosomes, and calculating the perpendicular distances from the sample to the multiple reference evolution vectors in the two-dimensional vector space, taking the sex chromosome variation path represented by the reference evolution vector with the smallest distance as the sex chromosome variation path of the sample to be tested; comparing and analyzing the overall copy number of the X / Y chromosome with the judgment threshold corresponding to the sex chromosome variation path, and outputting the sex chromosome variation detection result.

The invention provides a male fetus NIPT optimal time point determination and female fetus abnormity judgment method and system, and relates to the technical field of biomedicine. Preprocessing the NIPT detection data of the pregnant woman; extracting male fetus detection data in the pre-processed data, dividing the number of gestational weeks into a plurality of stages, respectively calculating the correlation between the Y chromosome concentration and the number of gestational weeks and between the Y chromosome concentration and the BMI of the pregnant woman in each stage, and constructing a relation model to obtain a correlation result between the Y chromosome concentration and the number of gestational weeks and between the Y chromosome concentration and the BMI of the pregnant woman; dividing the BMI of the male fetus and the pregnant woman into a plurality of groups, obtaining detection related indexes of each group under different gestational weeks through a survival analysis method by combining an association result and fusing multiple factors, constructing a multi-target planning model, and solving an optimal NIPT time point of each group of pregnant women; and extracting female fetus detection data in the preprocessed data, obtaining influence features through feature statistical analysis and significance screening, constructing a logistic regression model, and judging whether the female fetus is abnormal or not according to a model output result. The detection accuracy and the clinical auxiliary intervention timeliness are realized.

The application discloses a RNAi interference fragment targeting a donkey Zfy gene, a carrier and a preparation method and application thereof, and relates to the technical field of animal molecular genetics, wherein the RNAi interference fragment comprises RNAi-3-F and RNAi-3-R, and the nucleotide sequences are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The RNAi interference fragment provided by the application can target and silence Y chromosome sex determination related genes in male donkey Y spermatogenesis stage key reproductive cells, hinder and interfere with the normal development and physiological functions of Y sperm, regulate the key genes of Y sperm development through specific silencing, realize sex regulation before fertilization, greatly improve the proportion of female individuals of offspring donkeys, and the scheme does not damage target sperm cells and does not reduce sperm activity, and meanwhile, the scheme has the advantages of simple operation and low production cost.