Reagent and kit for detecting sexual accompanying hereditary disease of fetus

A detection kit and technology for genetic diseases, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of unfavorable pregnancy, improvement, and unfavorable detection of fetal sex-linked genetic diseases, etc., to achieve The effect of low cost and high detection accuracy

Pending Publication Date: 2022-03-01
深圳知因细胞生物科技有限公司
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Problems solved by technology

[0003] However, the traditional method of fetal gender detection is mainly through four-dimensional or color Doppler ultrasound. Clinically, the fetus needs to grow to a certain extent before it can be accurately judged, which is extremely unfavorable to pregnant women.
Moreover, the accuracy rate of judging the sex of the fetus by four-dimensional or color Doppler ultrasound is only about 85%, which is not conducive to the detection of fetal sex-linked genetic diseases
[0004] In addition, invasive testing methods such as amniocentesis can also be used to detect fetal g

Method used

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Embodiment

[0030] 1. Design of primers and probes

[0031] Specific detection primers and probes were designed for the Y chromosome and the housekeeping gene. The specific sequences are shown in Table 1. All primers and probes were synthesized by Shanghai Sangong.

[0032] Table 1 Primers and Probes

[0033] Numbering Sequence (5'→3') Seq ID No. Y-F AGACCAAGGAATGACCA 1 Y-R CAGGATTGCTGAGGTG 2 Y-P 5'FAM-CATGATGCCCCACGGC-3'BHQ2 3 G-F ACGACCACTTTGTCAA 4 G-R GCCAGACCCTGCACTTT 5 G-P 5'HEX-GGCTGGGGCCAGAGACT-3'BHQ2 6

[0034] In Table 1, those starting with "Y" are Y chromosome-specific primers and Y chromosome-specific probes, and those starting with "G" are housekeeping gene-specific primers and housekeeping gene-specific probes. "F" indicates an upstream primer, "R" indicates a downstream primer, and "P" indicates a probe. The fluorescent group of the Y chromosome specific probe is FAM, the quenching group is BHQ2, the fluores...

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Abstract

The invention discloses a reagent and a kit for detecting sex-associated hereditary diseases of fetuses. The reagent for detecting the sex-associated hereditary disease of the fetus comprises a Y chromosome specific primer and a Y chromosome specific probe, an upstream primer of the Y chromosome specific primer is Seq ID No.1, a downstream primer of the Y chromosome specific primer is a sequence shown as Seq ID No.2, and the Y chromosome specific probe is a probe with a fluorescent group at the 5'end and a quenching group at the 3 'end of a sequence shown as Seq ID No.3. According to the reagent for detecting the sex-associated hereditary disease of the fetus, the sex of the fetus can be judged by detecting blood of a pregnant woman, and the sex-associated hereditary disease is detected; the sex-associated hereditary disease related to the gender of the fetus can be found as soon as possible in the early pregnancy stage, and reference is provided for related treatment.

Description

technical field [0001] The invention relates to the field of early fetal detection, in particular to a reagent and kit for detecting fetal sex-associated genetic diseases. Background technique [0002] Fetal sex-linked genetic diseases refer to genetic diseases related to fetal sex, such as congenital agammaglobulinemia, X-linked cerebral edema, or mental retardation. Among them, X-chromosome recessive inheritance and Y-chromosome inheritance can be directly judged by detecting the sex of the fetus. [0003] However, the traditional method of fetal gender detection is mainly through four-dimensional or color Doppler ultrasound. Clinically, the fetus needs to grow to a certain extent before it can be accurately judged, which is extremely unfavorable to pregnant women. Moreover, the accuracy rate of judging the sex of the fetus by four-dimensional or color Doppler ultrasound is only about 85%, which is not conducive to the detection of fetal sex-linked genetic diseases. [0...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6879C12Q1/6851C12N15/11
CPCC12Q1/6883C12Q1/6879C12Q1/6851C12Q2600/166C12Q2531/113C12Q2545/101C12Q2561/101
Inventor 徐驰陶勇赵金凤李稳王美锦
Owner 深圳知因细胞生物科技有限公司
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