34 results about "Fetal sex" patented technology

The invention discloses a method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of a pregnant woman. The method comprises the following steps: 1, preprocessing fresh pregnant woman maternal peripheral blood: separating and enriching monocytes containing fetal nucleated red blood cells from maternal peripheral whole blood through a density gradient centrifugation technology; 2, capturing cells: adding a separated monocyte suspension to a substrate chip modified with an antibody, and carrying out standing capturing; and 3, identifying the sex of a fetus: identifying whether the nucleated red blood cells of the captured fetus contain Y chromosome or not by using an FISH probe technology (CSP-X / Y). The method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of the pregnant woman can realize capture and identification of the fetal nucleated red blood cells from the maternal peripheral circulating blood, and allows the FISH analysis to be directly carried out in order to realize noninvasive antenatal diagnosis of the sex or 21-Trisomy syndrome and other chromosome diseases of the fetus.

Methods, apparatuses, and system are provided for analyzing a maternal sample to determine whether a male fetus of a pregnant female has inherited an X-linked mutation from the mother. A percentage of fetal DNA in the sample is obtained, and cutoff values for the two possibilities (fetus inherits mutant or normal allele) are determined. A proportion of mutant alleles relative to a normal allele on the X-chromosome can then be compared to the cutoff values to make a classification of which allele is inherited. Alternatively, a number of alleles from a target region on the X-chromosome can be compared to a number of alleles from a reference region on the X-chromosome to identify a deletion or amplification. The fetal DNA percentage can be computed by counting reactions with a fetal-specific allele, and correcting the number to account for a statistical distribution among the reactions.

Provided herein are devices, systems, kits and methods for predicting or determining the gender of a fetus using cell free fetal nucleic acids in a small amount of maternal biological sample. Devices can be used at point of need during early stages of pregnancy and are compatible with communication devices.

The present invention provides methods for non-invasive determination of sex in a fetus or of Y chromosomal frequency abnormalities—indicative of aneuploidy or sex mosaicisms in a fetus—by detecting and determining the relative contribution genetic sequences from the Y chromosome in view of the percent fetal contribution in a maternal mixed sample.

The present invention provides methods for non-invasive determination of sex in a fetus or of Y chromosomal frequency abnormalities—indicative of aneuploidy or sex mosaicisms in a fetus—by detecting and determining the relative contribution genetic sequences from the Y chromosome in view of the percent fetal contribution in a maternal mixed sample.

The present invention relates to the technical field of gene identification and specifically discloses primers, probes, an identification method and a kit for identifying fetal chromosome sex. The primers and probes for identifying the fetal sex are primers and probes designed according to a male sex determining gene SRY, and three pairs of the primers and probes designed according to gene sequences of a, b, and c regions in an AZF region of Y chromosome, respectively. The plurality of pairs of the primers and corresponding probes are designed and a four-channel fluorescence detection method is used to determine existence of the SRYgene and the Y chromosome in a detection process, and the method has characteristics of high accuracy, high detection flux and high sensitivity.

A computer implemented method of early prediction of risk of pre-eclampsia in a pregnant obese woman is described. The method comprises the steps of inputting abundance values for a panel of obese pregnancy specific metabolite biomarkers obtained from an assayed biological sample into a computational model, in which the biological sample is obtained from an obese pregnant woman at 8 to 24 weeks of pregnancy, and inputting a patient parameter for the pregnant obese woman into the computational model selected from at least one of ethnicity, risk of gestational diabetes, fetal sex, number of pregnancies and level of obesity. The computational model is configured to select a subset comprising at least two of the obese pregnancy specific metabolite biomarkers based on the patient parameter input, correlate abundance values for the subset of obese pregnancy specific metabolite biomarkers with risk of pre-eclampsia, and output a predicted risk of pre-eclampsia for the pregnant obese woman.

The present invention relates to a method capable of, in order to diagnose fetal sex chromosome aneuploidy, differentiating Kleinfeiter's syndrome (XXY), triple X syndrome (XXX), and Turner's syndrome (monosomy X, XO) as well as male (XY) and female (XX) by using copy number variation (CNV). The differentiation method according to the present invention has significantly high sensitivity and accuracy since the reference line is evenly adjusted by performing normalization regardless of the kinds of platform and data. The present invention is useful in diagnosing the sex chromosome abnormality at an early stage through easy diagnosis of sex chromosomes X and Y, which are hard to diagnose, since an analysis is possible even with a small amount of fetal chromosomes, which corresponds to an advantage of noninvasive prenatal diagnosis, and copies are redundant.