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Primers, probes, identification method and kit for identifying fetal chromosome sex in early pregnancy

A chromosome and pregnancy identification technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of cumbersome operation, template amplification, low detection throughput, etc., and prolong the detection time. , to ensure the accuracy, the effect of high detection throughput

Active Publication Date: 2019-12-24
THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the existing method of fetal chromosome sex identification through fetal cell-free DNA in maternal plasma after 6 weeks of pregnancy, there are low detection sensitivity, easy failure of template amplification, high identification cost, low detection throughput of traditional fluorescent quantitative PCR method, and difficulty in setting internal reference , cumbersome operation, etc., the present invention provides a primer and probe, identification method and kit for identifying fetal chromosome sex

Method used

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  • Primers, probes, identification method and kit for identifying fetal chromosome sex in early pregnancy
  • Primers, probes, identification method and kit for identifying fetal chromosome sex in early pregnancy
  • Primers, probes, identification method and kit for identifying fetal chromosome sex in early pregnancy

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Embodiment 1

[0088] A primer and probe for identifying the chromosomal sex of a fetus in the first trimester, comprising

[0089] Design upstream primer SEQ ID NO:1, downstream primer SEQ ID NO:2 and probe SEQ ID NO:3 according to male sex determination gene SRY;

[0090] The upstream primer SEQ ID NO: 4, the downstream primer SEQ ID NO: 5 and the probe SEQ ID NO: 6 designed according to the a region in the Y chromosome AZF gene sequence;

[0091] The upstream primer SEQ ID NO: 7, the downstream primer SEQ ID NO: 8 and the probe SEQ ID NO: 9 designed according to the b region in the Y chromosome AZF gene sequence;

[0092] The upstream primer SEQ ID NO: 10, the downstream primer SEQ ID NO: 11 and the probe SEQ ID NO: 12 designed according to the c region in the Y chromosome AZF gene sequence;

[0093] Wherein the fluorescent group carried by SEQ ID NO:3 is selected from FAM, the fluorescent group carried by SEQ ID NO:6 is selected from VIC, the fluorescent group carried by SEQ ID NO:9 is ...

Embodiment 2

[0134] A primer and probe for identifying the sex of a fetus in the first trimester, comprising

[0135] The upstream primer SEQ ID NO:1, the downstream primer SEQ ID NO:2 and the probe SEQ ID NO:3 designed according to the male sex determining gene SRY;

[0136] The upstream primer SEQ ID NO: 4, the downstream primer SEQ ID NO: 5 and the probe SEQ ID NO: 6 designed according to the a region in the Y chromosome AZF gene sequence;

[0137] The upstream primer SEQ ID NO: 7, the downstream primer SEQ ID NO: 8 and the probe SEQ ID NO: 9 designed according to the b region in the Y chromosome AZF gene sequence;

[0138] The upstream primer SEQ ID NO: 10, the downstream primer SEQ ID NO: 11 and the probe SEQ ID NO: 12 designed according to the c region in the Y chromosome AZF gene sequence;

[0139] Wherein the fluorescent group carried by SEQ ID NO:3 is FAM, the fluorescent group carried by SEQ ID NO:6 is VIC, the fluorescent group carried by SEQ ID NO:9 is ROX, and the fluorescent...

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Abstract

The present invention relates to the technical field of gene identification and specifically discloses primers, probes, an identification method and a kit for identifying fetal chromosome sex. The primers and probes for identifying the fetal sex are primers and probes designed according to a male sex determining gene SRY, and three pairs of the primers and probes designed according to gene sequences of a, b, and c regions in an AZF region of Y chromosome, respectively. The plurality of pairs of the primers and corresponding probes are designed and a four-channel fluorescence detection method is used to determine existence of the SRYgene and the Y chromosome in a detection process, and the method has characteristics of high accuracy, high detection flux and high sensitivity.

Description

technical field [0001] The invention relates to the technical field of gene identification, in particular to primers and probes, an identification method and a kit for identifying the chromosome sex of a fetus. Background technique [0002] In order to reduce the incidence of sex-linked genetic diseases and improve the genetic quality of the population and the quality of the birth population, fetal sex identification technology during pregnancy has become one of the necessary technical means for risk assessment. [0003] At present, the prenatal identification of fetal sex is divided into invasive and non-invasive. Invasive prenatal diagnosis is mainly amniocentesis, which has certain risks to the fetus and pregnant women, and can easily lead to intrauterine infection and miscarriage. , fetal death and other adverse reactions are not easily accepted by pregnant women and their families; non-invasive prenatal diagnosis is mainly ultrasonic imaging diagnostic technology, this ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6879C12Q1/6851C12Q1/6888C12N15/11
CPCC12Q1/6851C12Q1/6879C12Q1/6888C12Q2600/16C12Q2531/113C12Q2563/107C12Q2537/143
Inventor 周英杰刘鹏刘学军刘慈
Owner THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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