315 results about "Genetic Materials" patented technology

The invention relates to the technical field of crop genetic breeding and plant molecular design breeding, in particular to a pakchoi whole genome liquid phase chip and application. The pakchoi breeding gene chip disclosed by the invention comprises a target capture probe which is designed by taking 1000 core SNP (Single Nucleotide Polymorphism) molecular markers positioned on 40KSNP sites on a pakchoi reference genome version V4.0 as templates. The pakchoi whole genome liquid phase chip provided by the invention comprises a 40K site targeted capture probe mixed solution and a hybridization capture reagent. The pakchoi 40KSNP targeted capture probe can rapidly and effectively track genetic materials of pakchoi, and is suitable for different application scenes such as accurate identification of pakchoi germplasm resources, genetic relationship analysis between materials, variety authenticity identification, QTL gene positioning and mining, molecular marker-assisted breeding, variety improvement and whole genome selective breeding application. Therefore, the method has good application prospect and important social value.

The invention belongs to the technical field of gene engineering, and particularly relates to a CaNAC02 gene for regulating and controlling synthesis of carotenoid in pepper fruits and application, and the amino acid sequence of protein encoded by the CaNAC02 gene is as shown in SEQ ID NO.1. The CaNAC02 gene disclosed by the invention has the effects of promoting synthesis of carotenoid compounds, influencing quality formation of the pepper fruits and the like in the pepper fruits. The CaNAC02 gene provided by the invention is an NAC family transcription factor, can provide a target gene for cultivation of a new pepper variety, and provides a genetic material and a theoretical basis for genetic improvement of the pepper variety.

The invention discloses a corn whole genome SNP (Single Nucleotide Polymorphism) site combination, a probe, a liquid chip and application thereof, the corn 10K whole genome liquid chip is developed based on a genome of a corn inbred line T32, SNP sites targeted by the corn 10K whole genome liquid chip uniformly cover a chromosome group, and key functional molecular marker SNP site markers of most important characters are more comprehensively included; compared with the prior art, the method has the advantages that more sites are provided, the detection range is more comprehensive, the detection rate is higher, and the repeatability is better. The target capture probe involved based on 10k SNP site information can rapidly and effectively track genetic materials of corn, the corn breeding process is accelerated, and the new variety breeding efficiency is improved.

The invention provides a chicken infectious laryngotracheitis subunit vaccine and application thereof, and belongs to the technical field of vaccine preparation. The chicken infectious laryngotracheitis subunit vaccine provided by the invention is prepared from chicken infectious laryngotracheitis virus gB-A463P protein coded by a gB-A463P gene of which the 463th alanine of an optimized chicken infectious laryngotracheitis gB gene is further mutated into proline, and the amino acid sequence of the gB-A463P protein is shown as SEQ ID NO: 5. The subunit vaccine is prepared by adopting a genetic engineering means, only contains part of antigens of the ILTV and does not contain genetic materials, so that the risks of toxin expelling and toxin dispersing do not exist, and the safety can be effectively improved.

The present invention relates to the field of biomedical materials, and in particular to a method for preparing a decellularized cartilage matrix material derived from pig rib cartilage, comprising the following steps: (1) freezing and thawing the pig rib cartilage for 5 times; (2) soaking the pig rib cartilage frozen and thawed in step (1) in a sodium borohydride solution; (3) soaking the pig rib cartilage treated with sodium borohydride in step (2) in a sodium dodecyl sulfate solution; and (4) enzymolyzing the pig rib cartilage obtained in step (3) with a mixed solution of DNase and RNase. The present invention is suitable for decellularization of cartilage tissue with a thickness of 300 microns, and utilizes the spontaneous generation of hydrogen by the sodium borohydride solution to effectively expand the cartilage tissue, and then the surfactant SDS fully destroys the cell membrane, effectively removes the genetic material, and retains the effective components or extracellular matrix of the natural cartilage to the greatest extent.

The present invention generally relates to systems (100) and methods for generating, managing, and displaying non-fungible digital representations (102) of living, biological, and / or eukaryotic organisms (104). More specifically, the systems (100) and methods for generating, managing, and displaying non-fungible digital representations (102) include genetic material (106), such as single nucleotide polymorphisms. The systems (100) and methods can record genetic data from the genetic material (106) on a blockchain (114) and can generate and display the non-fungible digital representation (102) of the living, biological, and / or eukaryotic organisms (104) that are based on the genetic data on the blockchain (114).

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The invention provides a discharging device and sterilization equipment, the discharging device comprises a first voltage part and a second voltage part, an interval channel is arranged between the first voltage part and the second voltage part, the first voltage part is provided with a plurality of air inlets penetrating through the two sides of the first voltage part, and the second voltage part is provided with a plurality of air outlets penetrating through the two sides of the second voltage part; air enters the interval channel from one side of the first voltage piece through the air inlet hole, is ionized and then flows out through the air outlet hole. Air is ionized in the interval channel between the first voltage part and the second voltage part to form gas-phase active particles and free radicals with strong oxidizing property, and the gas-phase active particles and free radicals can destroy cell membranes, enzyme systems and genetic materials of microorganisms, so that the microorganisms in the space are efficiently killed, and the safety of the space is improved. Therefore, a relatively strong bacteriostatic and bactericidal effect is achieved; meanwhile, in the sterilization and bacteriostasis process of the discharging device, toxic substances harmful to the human body cannot be generated, the green and harmless sterilization effect is achieved, and man-machine coexistence is achieved.

The invention discloses a deep-sea parallel microorganism in-situ pressure-maintaining filtering device and method. The deep-sea parallel microorganism in-situ pressure-maintaining filtering device comprises a reversing module, a liquid pumping monitoring module and at least one acquisition monitoring module, the reversing module comprises a silt filter, an RNAlater flexible bag and an electromagnetic reversing valve; the liquid pumping monitoring module comprises a first three-way connector, a differential pressure sensor, a pump, an overflow valve and a turbine flowmeter. In the acquisition monitoring module, an inlet of a one-way valve is connected with an outlet of a turbine flowmeter, and an outlet of the one-way valve is connected with an inlet of an intermediate plate on the side surface of the microbial filter through a first stop valve; the microbial filter is provided with a pressure sensor and a piston pressure supplementing device; the inlet end of the piston pressure supplementing device is connected with a second stop valve; bottom and top outlets of the microbial filter are connected with a second three-way connector, and the other end of the second three-way connector is connected with an electromagnetic one-way valve through a third stop valve. By utilizing the method, genetic materials and cell structures of microorganisms can be prevented from being damaged to the maximum extent in the recycling and transferring process.

The invention belongs to the field of cell culture, and relates to an MRC-5 cell serum-free medium with definite components and application thereof. Nutrient substances of the serum-free culture medium comprise amino acids, vitamins, inorganic salts, trace elements, proteins, other substances and supplements. According to the MRC-5 cell serum-free culture medium, the concentration of the amino acid, the inorganic salt, the vitamin and the serum substitute is adjusted, and meanwhile, the Chinese yam glycopeptide is added, so that adherent growth of MRC-5 cells can be effectively promoted, and the technical problem that a new variety of MRC-5 cell serum-free culture medium is lacked in the prior art is solved; the requirements of nutrient substances, carbon sources and genetic material nutrient substances required by MRC-5 cell in-vitro culture can be met.

The invention provides a preparation method of a recombinant cell, the recombinant cell and application of the recombinant cell, and relates to the technical field of biology. A target protein expressed by the recombinant cell contains one, two, three or four polypeptide chains; the preparation method comprises the following steps: integrating exogenous polynucleotides for coding a target protein into a genetic material of a starting cell in a site-specific manner, wherein each exogenous polynucleotide for site-specific integration contains coding genes of all polypeptide chains of the target protein; each exogenous polynucleotide contains at least four expression cassettes, and each polypeptide chain is encoded by at least one expression cassette. The preparation method is simple to operate, coding genes of all polypeptide chains can be integrated into genetic materials of starting cells at a time, and the expression quantity of foreign proteins of the recombinant cells obtained through the preparation method is high.

Provided herein is a system for delivering genetical material, preferably subcutaneously, and its various elements. Also provided are related compositions and methods.

The invention discloses an implantable acellular matrix material and a preparation method thereof, and belongs to the field of biomedical materials. According to the method, natural animal tissues (such as pericardium, achilles tendon or small intestine submucosa) are used as raw materials, a low-temperature chemical combined decellularization treatment technology is adopted, and the method comprises the multi-step synergistic process of pre-cooling soaking balancing, low-temperature enzymolysis, low-concentration surfactant gradient permeation cleaning, low-temperature nuclease degradation, non-crosslinking sterilization and the like. Under the condition that the treatment temperature is strictly kept at 0-8 DEG C, cell components and genetic materials (the residual DNA content is 1t, and the dry weight is 8 ng / mg) are efficiently removed, and meanwhile the natural three-dimensional fine structure of an extracellular matrix (ECM), the integrity of collagen fibrils and key bioactive components (such as glycosaminoglycans (GAGs)) are reserved to the maximum extent. The host immunological rejection reaction of the obtained material is remarkably reduced, host cell ingrowth, vascularization and tissue function reconstruction can be effectively promoted after implantation, and the material is suitable for high-end implantation scenes such as soft tissue repair and regenerative medical stents.

Artificial intelligence driven enhancement of motion blurred sequencing images enables enhanced sequencing that determines a sequence of bases in genetic material with any one or more of: improved performance, improved accuracy, and / or reduced cost. A training set of images taken after unreduced and reduced movement settling times during sequencing is used to train a neural network to enable the neural network to recover enhanced images, as if taken after the unreduced movement settling time, from unenhanced images taken after the reduced movement settling time.

This invention discloses the application of the apple MdWRI1 gene in regulating the wax content of apple fruits and leaves, belonging to the field of plant genetic engineering technology. This invention isolates an MdWRI1 gene with high expression levels in apple varieties with high wax content, its nucleotide sequence of which is shown in SEQ ID NO.1. Subcellular localization revealed that the transcription factor expressing the MdWRI1 gene is located in the cell nucleus. Experimental results show that overexpression of the MdWRI1 gene can significantly increase the wax content of apple fruits and leaves by promoting wax biosynthesis, indicating that the MdWRI1 gene plays a key role in regulating the wax content of apple fruits and leaves. This invention provides an efficient and rapid approach for apple breeding, provides genetic material for improving apple quality, and has broad application prospects in improving the economic and ecological benefits of apple cultivation.

The invention relates to a vessel for the long-term storage of DNA in space at a constant temperature of -196 °C. The aim is to protect biological samples – especially DNA – for decades to centuries from the extreme environmental conditions of space, including vacuum, intense radiation, extreme temperature fluctuations and mechanical stresses. The vessel has a three-layered structure: 1. Inner chamber made of boron nitrogen nanotubes (BNNTs) that offer outstanding protection against cosmic radiation, high mechanical strength, chemical inertness and temperature resistance. 2. Middle insulating layer made of aerogel that minimizes heat exchange and keeps the cryogenic temperature constant. 3. Outer protective layer made of titanium, which protects against micrometeorites, It protects against physical impacts and corrosion. Technical advantages: • Long-term stability of DNA through consistently low temperatures. • Effective radiation protection against cosmic and solar radiation. • Excellent thermal insulation thanks to aerogel. • Mechanical robustness due to titanium. Areas of application: • Securing and archiving genetic material for research, space travel and long-term preservation. • Provision of DNA for future scientific or biological restoration projects. Special feature: The capsule is compact, space-compatible, and can be used as part of space missions, space stations, or interplanetary projects.

The invention discloses a water pollution source identification and source tracing method based on an environmental DNA macro bar code, which comprises the following steps: S1: eDNA sample collection: collecting a water sample through an alcohol preservation sample method, and collecting genetic materials in an aquatic environment by collecting the water sample and filtering instead of sampling an organism; s2, eDNA extraction: extracting eDNA by a CTAB (cetyltrimethyl ammonium bromide) method or a kit. According to the water pollution source identification and traceability method based on the environmental DNA macro bar code, through an eDNA macro bar code detection method, the specialty level requirement for species classification of researchers is not high; especially for plankton, part of benthic organisms, larvae and other objects which are difficult to identify by non-professionals, the eDNA macro bar code detection method has relatively high operability; for rare and endangered organisms with a small number of individuals, the eDNA macro bar code detection method does not need to catch target organisms, so that the operation process is greatly simplified.

A duchenne muscular dystrophy-related exonic splicing enhancer, sgRNA and gene editing tool can be applied in the preparation of drugs for treating duchenne muscular dystrophy. The gene editing tool designed on the basis of cytosine deaminase AID mutants and Cas9 mutants can perform site-specific modification on a mammalian genome by using an adeno-associated virus (AAV) as a vector. By optimizing an encoding nucleic acid sequence and an element composition structure of the editing tool, site-specific targeted modification of mammalian genetic material DNA can be efficiently achieved; and by performing targeted genetic manipulation on the nucleic acid sequence carrying disease mutations, a pathogenic mutation cannot be retained in a mature protein amino acid sequence or the pathogenic mutation cannot perform its function, so that the purpose of treating various gene mutation type genetic rare diseases is achieved, and the advantages of high efficiency, safety and stability are achieved.

Quick, user-centric, equipment-independent, and cost-efficient nucleic acid processing systems, compositions and devices, as well as methods of use thereof, for isothermal nucleotide amplification of single-stranded amplicons and double-stranded ampliconsand inhibition of non¬ specific binding are described herein. The nucleic acid processing device is proficient in the seamless extraction, amplification, and detection of genetic material from biological or environmental samples. The methods, compositions, and systems herein do not require thermal melting and may be used in a point-of-need setting. The methods feature the amplification of a target region and the production of single -stranded amplicons including the target region, bound by an in-reaction probe.

The invention relates to a hybrid nanocarrier system comprising engineered metal nanoparticles (MeNP), a flavonoid (Fl), a Raman-active molecule (RAM), poly(allylamine hydrochloride) (PAH), genetic material (GM), and poly(styrene sulfonate) (PSS), designed for use in fields such as gene therapy, cancer treatment, chemotherapy, treatment of genetic diseases, innovative vaccine technologies, the production of innovative antibiotics for antibacterial and antimicrobial therapies, drug delivery, the dietary supplement market, Raman imaging systems, biotechnological applications, and the agrochemical industry.

To provide a humanized rodent that expresses a heavy chain containing a VL domain. [Solution] A non-human animal, tissue, cell, and genetic material is provided which includes a modification of an endogenous non-human heavy chain immunoglobulin sequence and contains functional ADAM6 activity in a rodent (e.g., mouse), wherein the non-human animal rearranges the human immunoglobulin light chain gene segment in relation to the heavy chain constant domain and expresses an immunoglobulin-like molecule which contains a human immunoglobulin light chain variable domain fused to the heavy chain constant domain and is a cognitive of a human immunoglobulin light chain variable domain fused to the light chain constant domain.

Methods, systems, and apparatuses to encode data for storage in genetic materials. For example, a computing system may segment user data into a plurality of data blocks and generate seed data characterizing a plurality of fountain code seeds. Additionally, the computing system may, for each data block, implement a set of operations that generate one or more data packets. In some instances, the set of operations may include, for each of the plurality of fountain code seeds, determining a bit value and corresponding metaCode value and determining which of the fountain code seeds has a metaCode value of the bit value that matches a value of the bit position identified in the metadata. Moreover, the computing system may, for each data packet, cause an implementation of a second set of operations that synthesize a polynucleotide strand in accordance with at least bit values of the corresponding data packet.

The invention belongs to the related technical field of digital microfluidics, and discloses a digital microfluidics MALBAC device based on temperature measurement and control, which comprises a digital microfluidics chip 10 and a temperature measurement and control system 20, the digital micro-fluidic chip 10 adopts a closed structure and comprises an upper cover plate 111 and a lower chip 112 which are arranged in parallel relatively, and the temperature measurement and control system 20 comprises a temperature conduction module 21 and a temperature sensing module 22; according to the device, a totally-closed operation environment is provided for the MALBAC process, the risk of cross contamination is effectively avoided, the device can create a plurality of independent temperature areas on a single chip, the thermal cycle efficiency in the MALBAC technology is optimized, it is ensured that the temperature requirement of each stage in the amplification process is accurately met, and the amplification efficiency is improved. The integration level, the operation flexibility and the expandability of the device are greatly improved, so that the device can adapt to library construction requirements of various genetic material samples.

The invention discloses a PDCoV-S1 recombinant protein antigen as well as a preparation method and a vaccine thereof, and belongs to the technical field of recombinant protein vaccines. The amino acid sequence of the PDCoV-S1 recombinant protein antigen disclosed by the invention is as shown in SEQ ID NO: 1; the nucleotide sequence of the PDCoV-S1 recombinant protein antigen is as shown in SEQ ID NO: 2. The vaccine provided by the invention only contains recombinant PDCoV-S1 protein and does not contain genetic materials of viruses, so that all risks of virus replication, virulence reversion or gene recombination are fundamentally avoided. Live viruses do not need to be operated in the production process, so that the biological safety risk and the requirement on the production environment are greatly reduced. The constructed stable CHO engineering cell strain is combined with a serum-free suspension culture technology, so that high-density, large-scale and standardized production of the PDCoV-S1 protein can be realized in a bioreactor. The production process is stable and reliable, the batch-to-batch consistency is good, and the uncertainty and volatility caused by the virus culture link of the traditional vaccine are overcome.

The invention discloses a SbASR4 gene for regulating and controlling plant salt stress tolerance as well as an encoding protein and application thereof. The SbASR4 gene is derived from Sorghum baicola, and the encoded protein is simultaneously positioned in a cell membrane and a cell nucleus, does not have transcriptional activation activity, and is a negative regulation factor of plant salt stress response. The overexpression of the gene can significantly increase the sensitivity of the plant to salt and ABA, and the silence or knockout of the gene can greatly improve the salt tolerance of the plant. Mechanism research shows that the SbASR4 protein directly interacts with an SOS signal channel core kinase SOS2 in a plant body, and the SOS channel is negatively regulated through the interaction, so that the expression of a downstream ion transport gene is inhibited. The invention provides a method for improving the salt tolerance of plants by reducing the expression of SbASR4 or destroying the interaction of SbASR4 and SOS2, related genetic materials and products, and a brand new target and an effective strategy are provided for salt-resistant breeding of crops.

Reserves of immortalized genetic material are stored in a bank for providing a resource (e.g., artificial gametes) for couples (e.g., same sex couples) to produce biologically-related children. The reserves provide the ability to derive sperm from induced pluripotent stem cells (iPSC's) of one partner and / or eggs from iPSC's of the other partner. For example, a biological sample is stored as iPSC's that can be used to generate an unlimited supply of genetic material (e.g., artificial gametes for conception) when needed by a user (e.g., a year or more after the sample is provided, e.g., 5 years or more after the sample is provided). Such a bank is helpful for an individual in a same sex (or infertile) couple who desires to have biological children at some point during his / her lifetime, but does not plan to have children in the immediate timeframe, for example.

The utility model belongs to the technical field of genetic material extraction appliance, concretely relates to special centrifugal tube suit of south American white prawn nucleic acid extraction, including the pipe body, the top threaded connection of pipe body has the pipe cap, the position department of pipe body outer wall is close to the fixed setting of mounting ring of top, the outer wall of pipe cap is below the sliding ring of mounting ring and is connected with the damping sliding, the position department of mounting ring bottom close to the outside edge is hinged with four upper articulated rods at equal intervals, the top of sliding ring is hinged with the lower articulated rod of corresponding position of upper articulated rod, and the corresponding upper articulated rod is hinged with the lower articulated rod between setting articulated assembly. The utility model places the pipe body on the test tube rack through the amplitude of the lower articulated rod and the upper articulated rod protruding from the outside of the pipe body to support the pipe body, so that the pipe body can be adapted to different diameter types of test tube racks, increase the types of centrifugal tubes that can be adapted to test tube racks, facilitate the use of centrifugal tubes, and at the same time, reduce the types of test tube racks that need to be purchased, and reduce the cost of purchasing test tube racks.

The present application relates to a method for identifying a sex antagonistic hotspot region on a pig autosomal chromosome and application thereof. The method provided by the present application comprises the following specific steps: constructing a hybrid population F0, F1, F2 three generations, genome sequencing and SNP identification, transmission tracking of genetic material in the hybrid population pedigree, and identifying a sex antagonistic hotspot region. The method of the present application identifies that the Chr3:121M window is a sex ratio directional regulation molecular marker that is beneficial to the generation of male offspring in the Large White / Mini pig genotype individual. According to the method of the present application, the pig population sex can be predicted and regulated, and by screening and optimal mating of the genotype of the parent before mating, a plurality of generations is obtained by continuous cross without introducing exogenous blood relationship, so that the maximum value of the proportion of the genotype beneficial to the male or female is achieved under the condition that all genotypes of each generation are retained at the population level, and then the dynamic regulation of the sex ratio of the offspring pig population is achieved.

The present invention relates to a composition for delivering functional substances and uses thereof. The composition for delivering functional substances using non-vesicular extracellular particles according to the present invention can effectively load genetic materials such as proteins, mRNA, and / or pDNA and efficiently deliver same to cells, and thus can be used as a pharmaceutical composition or a functional cosmetic composition for treating diseases.