117 results about "Genetic Materials" patented technology

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

This invention discloses the application of the apple MdWRI1 gene in regulating the wax content of apple fruits and leaves, belonging to the field of plant genetic engineering technology. This invention isolates an MdWRI1 gene with high expression levels in apple varieties with high wax content, its nucleotide sequence of which is shown in SEQ ID NO.1. Subcellular localization revealed that the transcription factor expressing the MdWRI1 gene is located in the cell nucleus. Experimental results show that overexpression of the MdWRI1 gene can significantly increase the wax content of apple fruits and leaves by promoting wax biosynthesis, indicating that the MdWRI1 gene plays a key role in regulating the wax content of apple fruits and leaves. This invention provides an efficient and rapid approach for apple breeding, provides genetic material for improving apple quality, and has broad application prospects in improving the economic and ecological benefits of apple cultivation.

A duchenne muscular dystrophy-related exonic splicing enhancer, sgRNA and gene editing tool can be applied in the preparation of drugs for treating duchenne muscular dystrophy. The gene editing tool designed on the basis of cytosine deaminase AID mutants and Cas9 mutants can perform site-specific modification on a mammalian genome by using an adeno-associated virus (AAV) as a vector. By optimizing an encoding nucleic acid sequence and an element composition structure of the editing tool, site-specific targeted modification of mammalian genetic material DNA can be efficiently achieved; and by performing targeted genetic manipulation on the nucleic acid sequence carrying disease mutations, a pathogenic mutation cannot be retained in a mature protein amino acid sequence or the pathogenic mutation cannot perform its function, so that the purpose of treating various gene mutation type genetic rare diseases is achieved, and the advantages of high efficiency, safety and stability are achieved.

The invention relates to a hybrid nanocarrier system comprising engineered metal nanoparticles (MeNP), a flavonoid (Fl), a Raman-active molecule (RAM), poly(allylamine hydrochloride) (PAH), genetic material (GM), and poly(styrene sulfonate) (PSS), designed for use in fields such as gene therapy, cancer treatment, chemotherapy, treatment of genetic diseases, innovative vaccine technologies, the production of innovative antibiotics for antibacterial and antimicrobial therapies, drug delivery, the dietary supplement market, Raman imaging systems, biotechnological applications, and the agrochemical industry.

The invention discloses a PDCoV-S1 recombinant protein antigen as well as a preparation method and a vaccine thereof, and belongs to the technical field of recombinant protein vaccines. The amino acid sequence of the PDCoV-S1 recombinant protein antigen disclosed by the invention is as shown in SEQ ID NO: 1; the nucleotide sequence of the PDCoV-S1 recombinant protein antigen is as shown in SEQ ID NO: 2. The vaccine provided by the invention only contains recombinant PDCoV-S1 protein and does not contain genetic materials of viruses, so that all risks of virus replication, virulence reversion or gene recombination are fundamentally avoided. Live viruses do not need to be operated in the production process, so that the biological safety risk and the requirement on the production environment are greatly reduced. The constructed stable CHO engineering cell strain is combined with a serum-free suspension culture technology, so that high-density, large-scale and standardized production of the PDCoV-S1 protein can be realized in a bioreactor. The production process is stable and reliable, the batch-to-batch consistency is good, and the uncertainty and volatility caused by the virus culture link of the traditional vaccine are overcome.

The invention discloses a SbASR4 gene for regulating and controlling plant salt stress tolerance as well as an encoding protein and application thereof. The SbASR4 gene is derived from Sorghum baicola, and the encoded protein is simultaneously positioned in a cell membrane and a cell nucleus, does not have transcriptional activation activity, and is a negative regulation factor of plant salt stress response. The overexpression of the gene can significantly increase the sensitivity of the plant to salt and ABA, and the silence or knockout of the gene can greatly improve the salt tolerance of the plant. Mechanism research shows that the SbASR4 protein directly interacts with an SOS signal channel core kinase SOS2 in a plant body, and the SOS channel is negatively regulated through the interaction, so that the expression of a downstream ion transport gene is inhibited. The invention provides a method for improving the salt tolerance of plants by reducing the expression of SbASR4 or destroying the interaction of SbASR4 and SOS2, related genetic materials and products, and a brand new target and an effective strategy are provided for salt-resistant breeding of crops.

The utility model belongs to the technical field of genetic material extraction appliance, concretely relates to special centrifugal tube suit of south American white prawn nucleic acid extraction, including the pipe body, the top threaded connection of pipe body has the pipe cap, the position department of pipe body outer wall is close to the fixed setting of mounting ring of top, the outer wall of pipe cap is below the sliding ring of mounting ring and is connected with the damping sliding, the position department of mounting ring bottom close to the outside edge is hinged with four upper articulated rods at equal intervals, the top of sliding ring is hinged with the lower articulated rod of corresponding position of upper articulated rod, and the corresponding upper articulated rod is hinged with the lower articulated rod between setting articulated assembly. The utility model places the pipe body on the test tube rack through the amplitude of the lower articulated rod and the upper articulated rod protruding from the outside of the pipe body to support the pipe body, so that the pipe body can be adapted to different diameter types of test tube racks, increase the types of centrifugal tubes that can be adapted to test tube racks, facilitate the use of centrifugal tubes, and at the same time, reduce the types of test tube racks that need to be purchased, and reduce the cost of purchasing test tube racks.

The invention discloses a recombinant duck plague virus protein combination, a subunit vaccine as well as a preparation method and application of the subunit vaccine, and belongs to the technical field of vaccine preparation. According to the technical scheme, the recombinant duck plague virus protein combination comprises recombinant gE protein and recombinant gI protein; the amino acid sequence of the recombinant gE protein is as shown in SEQ ID NO: 10, and the amino acid sequence of the recombinant gI protein is as shown in SEQ ID NO: 13. The nucleotide sequence of the nucleic acid molecule for coding the recombinant gE protein is as shown in SEQ ID NO: 12; the nucleotide sequence of the nucleic acid molecule for coding the recombinant gI protein is as shown in SEQ ID NO: 15. The duck plague subunit vaccine provided by the invention adopts gE and gI co-expression, only contains part of antigens of DPV, does not contain genetic materials, does not have toxin expelling and dispersing risks, is good in safety, can enable the spatial conformation of gE protein to be close to that of natural protein by co-expression of gE and gI, can realize 10 / 10 protection of challenge, has the total protein expression amount of about 4000 [mu] g / ml, and can be used for preparing a vaccine for the duck plague. The culture medium can be diluted by 40 times for seedling production, so that the production cost is greatly reduced.

To provide a method for rapidly and accurately quantifying bacteria in a biological sample from a subject.SOLUTION: A method of determining an amount or concentration of bacteria in a sample, the method comprising: (a) amplifying a target nucleic acid of the bacteria from genetic material obtained from the sample to form an amplification product, wherein: (b) measuring the amount or concentration of the amplification product; (c) calculating the amount or concentration of the target nucleic acids in the sample by comparing the amount or concentration of the amplification product to a reference level thereof; and (d) determining the copy number of the bacterial 16SrRNA genes in the sample from the amount or concentration of the target nucleic acids in the sample, wherein: 16SrRNA, wherein said copy number is a function of or correlates with the amount of said bacteria in said sample.SELECTED DRAWING: None

A method for simultaneously detecting two or more target genetic materials present in a biological sample, wherein the method comprises: adding two or more Crispr-Cas systems capable of respectively targeting two or more target genetic materials and two or more probe molecules corresponding to the Crispr-Cas systems into the biological sample; when any of the Crispr-Cas systems encounters the target genetic material it targets, the Cas enzyme in the Crispr-Cas system can be activated by the target genetic material it targets to trans-cleave the probe molecule corresponding to it; detecting the fluorescence signal emitted by the trans-cleaved probe molecule to determine whether the target genetic material is present in the biological sample. Also related are the corresponding kits.

The invention relates to diagnostic methods, and particularly, although not exclusively, to the use of oligomeric protein traps in sample diagnostics. The invention extends to the use of oligomeric protein traps as binding agents, for example in methods for enriching samples of low-concentration proteins and / or genetic material, and methods for detecting the same in samples. The invention also encompasses high-affinity protein traps and their application in environmental monitoring, diagnosis and therapy.

The present invention relates to a method for analyzing genetic material in a subject. More specifically, it relates to a method for analyzing genetic material using unphased genotypic information of polymorphic variants from one parent and one parent, in combination with the allele frequency of these variants in the subject's genetic material. Furthermore, the method of the present invention is particularly useful for analyzing isolated genetic material from a sample with a low amount of genetic material and / or for detecting low-level chromosomal mosaicism.

A system, method, and apparatus for delivering genetic material to a shoot apical meristem. The delivery system may have a biolistic particle delivery assembly having a gas acceleration tube and a bombardment chamber. The bombardment chamber has a microcarrier launch assembly comprising an opening adapted to receive gas for acceleration of the microcarrier system and to direct gas to a target. The delivery system may have a source of pressurized carrier gas operatively connected to a first end of the gas acceleration tube; and an extender. The extender has a channel configured to fit through the opening of the microcarrier launch assembly. The length of the extender facilitates the penetration of the microcarrier system to an L2 layer of the shoot apical meristem, conferring germline transformation and / or modification.

This invention discloses a culture medium, preparation method, and culture method for the industrial-scale preparation of mesenchymal stem cells, belonging to the field of cell therapy technology. The culture medium comprises a basal medium, cell growth factors, cell proliferation promoters, a vitamin mixture, a nucleoside mixture, and an amino acid mixture; wherein the cell growth factors are selected from one or more of TGF-β, bFGF, VEGF, PDGF, EGF, and IGF. Through the synergistic effect of the composite nutrient system, this invention significantly improves the proliferation rate and cell viability of mesenchymal stem cells, maintains stem cell stemness, trilineage differentiation ability, and secretion levels of key cytokines (VEGF, HGF, TGF-β, IL-10, etc.); it can be stably and continuously passaged up to the 15th generation without abnormal changes in genetic material. This culture medium contains no animal-derived components, has high batch-to-batch stability, and is cost-controllable, making it suitable for GMP-level large-scale industrial preparation of mesenchymal stem cells from various sources such as umbilical cord, bone marrow, adipose tissue, and placenta.

This invention belongs to the field of radiation mutagenesis technology, and relates to a method for mutagenesis of lavender seeds, employing... 60 Lavender seeds are treated with Co-γ rays; the radiation dose is 50-500 Gy, and the dose rate is 1-3 Gy / min. This invention employs... 60 Radiation with Co-γ rays can efficiently induce mutations in the genetic material of lavender seeds, effectively breaking gene linkage and significantly increasing the mutation frequency. This provides a rich material basis for screening lavender mutants, allowing for the selection of seed half-lethal doses, and obtaining plants with variations in leaf color, leaf shape, plant height, and other traits. This provides technical support for lavender breeding using radiation mutagenesis technology.

This invention discloses a penicillinase mutant, its recombinant strain, and its applications, relating to the field of antibiotic wastewater treatment. Using the wild-type penicillinase of SEQ ID NO:1 as a template, this invention obtains penicillinase mutants of SEQ ID NO:2-7 through site-directed combination mutagenesis, significantly improving wide pH tolerance. While the wild-type enzyme experiences a sharp drop in activity at pH 9-10, the mutant of this invention maintains high enzyme activity at pH 5-10, reaching over 90% of the highest wild-type activity at pH 9-10. It can be directly adapted to the highly alkaline environment of penicillin G production wastewater without significant pH adjustment. This invention also provides the recombinant genetic material encoding the mutant, the recombinant strain, and its application in wastewater purification, exhibiting advantages such as high degradation efficiency, low operating costs, stable process, and environmental friendliness, possessing significant engineering application value in the treatment of penicillin antibiotic wastewater.

The present disclosure provides technologies for genetic modification that use a helicase beta-wing element (HbW element). Provided technologies enable genetic modification without a need for introduction of one or more breaks into any genetic material being modified.

A method of treating a subject having been diagnosed as having cancer with an immunotherapy is described. The method comprises targeting one or more clonal neoantigens selected using a method comprising determining whether a tumour-specific mutation is likely to be clonal in a subject. The method comprises providing sequence data from one or more samples from the subject comprising tumour genetic material, the sequence data comprising for each of the one or more samples, and determining the likelihood that the tumour-specific mutation is clonal as a posterior probability depending on: a prior probability of the mutation being clonal, and the probabilities of observing the sequence data if the tumour-specific mutation is (i) clonal and (ii) non-clonal, in view of a tumour fraction for each of the one or more samples and one or more candidate joint genotypes.

Mice, tissues, cells, and genetic material are provided that comprise a humanized heavy chain immunoglobulin locus, a humanized light chain locus that expresses a universal light chain, and a gene encoding an ADAM6 or ortholog or homolog or functional fragment thereof. Mice are provided that express humanized heavy chains comprising human variable domains, and that express humanized light chains comprising human variable domains wherein the light chains are derived from no more than one, or no more than two, light chain V and J or rearranged V / J sequences. Fertile male mice that express antibodies with universal light chains and humanized heavy chains are provided. Methods and compositions for making bispecific binding proteins are provided.

The invention discloses application of a premna microphylla turcz diene oxygenase gene NtPO, the nucleotide sequence of the premna microphylla turcz diene oxygenase gene NtPO is shown as SEQ ID NO.1, the premna microphylla turcz diene oxygenase gene NtPO contains 450 basic groups, the gene is derived from tobacco, and the premna microphylla turcz diene oxygenase gene NtPO is obtained by editing the NtPO gene. The topped plant height, stem girth, waist leaf length, waist leaf width and leaf area of the gene editing plant in the mature period are all higher than those of a control plant. In a word, according to the application, the NtPO gene is knocked out by utilizing a CRISPR / Cas9 mediated gene editing technology to obtain a gene editing plant which affects the growth and development of the plant, and a genetic material and a theoretical basis are provided for tobacco premna microphylla diene oxygenase gene research and tobacco growth and development research.

This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are a method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

Methods, systems, and devices for encoding data for storage in genetic material are disclosed. For example, a computing system can segment user data into a plurality of data blocks and generate seed data characterizing a plurality of fountain code seeds. Additionally, the computing system can, for each data block, implement a set of operations to generate one or more data packets. In some instances, the set of operations can include, for each of the plurality of fountain code seeds, determining a bit value and a corresponding meta code value, and determining which of the fountain code seeds has a meta code value for the bit value that matches a value for the bit position identified in the metadata. Furthermore, the computing system can, for each data packet, cause implementation of a second set of operations to synthesize a polynucleotide strand from at least the bit values of the corresponding data packet.

The application discloses a method for knocking out a tobacco NtLNP1 gene to obtain tobacco with changed leaf shape and low nicotine content and application thereof. A gene related to tobacco nicotine synthesis metabolism and leaf shape regulation is an NtLNP1 gene. Through a CRISPR / Cas9-mediated gene editing technology, a CRISPR / Cas9 editing vector for knocking out the NtLNP1 gene is constructed, and after editing material creation and molecular detection and identification, an editing plant with the NtLNP1 gene knocked out is obtained. The editing plant has an oval leaf shape, which is obviously changed compared with a control leaf shape. It is found through gas chromatography-mass spectrometry detection that the nicotine content of the leaf of the editing plant with the NtLNP1 gene knocked out at a budding stage is significantly lower than that of the control plant. The application provides genetic materials and a theoretical basis for research on functions of tobacco nicotine metabolism genes and directional improvement of new tobacco varieties with controllable nicotine content.

Non-viral gene therapy treatments have a number of advantages over viral vectors including typical non-antigenicity, low manufacturing cost, simplicity, and efficiency of delivering genetic cargo into the cytoplasm. The potency of non-viral gene therapy systems can be enhanced by providing them with means to more efficiently transport the delivered DNA from the cytoplasm to the nucleus. Provided herein, at least in part, are means of enhancing potency of non-viral gene therapies by including novel nuclear translocation enhancing sequences and compositions that can result in optimized nuclear translocation as well as related compositions and methods.

Reagents useful for immunotherapy and prevention of human cytomegalovirus (hCMV) infection are provided. The reagents are variant gB polypeptides (or genetic material encoding said polypeptides) wherein disulfide bridges are introduced into the amino acid sequence of gB to stabilize the pre-fusion conformation of said protein. The disulfide bridges are introduced via double substitution as shown in SEQ ID NO:1 and are selected from the group consisting of: 1) I103C and V645C; 2) A97C and A538C; and 3) G543C and K617C. Vaccine compositions and methods of using them are also disclosed.

Provided are tropic modified recombinant viral vectors and compositions comprising same that are useful for targeted transfer of genetic material into cells and / or tissues. [Solution] The present invention provides a recombinant viral capsid protein comprising an epitope, the epitope being heterologous to the capsid protein, the heterologous epitope or a portion thereof specifically binding to an antibody paratope, and the viral capsid protein forming a recombinant viral capsid with reduced or abolished native tropism. In one embodiment, the recombinant viral capsid protein comprises a substitution, insertion, or deletion at an amino acid position responsible for the native tropism of the viral capsid, such that the recombinant viral capsid protein forms a viral capsid with reduced or abolished native tropism.

The invention relates to the technical field of biological materials, in particular to a preparation method and application of a nucleus pulposus acellular matrix hydrogel material. The method comprises the following steps: taking a proper amount of lumbar nucleus pulposus tissues of pigs in a slaughter house, cleaning the lumbar nucleus pulposus tissues with PBS for several times, then taking part of the lumbar nucleus pulposus tissues for treatment, and subpackaging the residual nucleus pulposus tissues at-20 DEG C Preparing a decellularized liquid working solution; preparing an acellular nucleus pulposus matrix; cleaning the acellular nucleus pulposus matrix; evaluating acellular nucleus pulposus matrix genetic material residues and extracellular matrix preservation conditions; preparing a powdery nucleus pulposus acellular matrix; preparing a methacrylated acellular nucleus pulposus matrix; detecting the cross-linking performance of the methacrylated acellular nucleus pulposus matrix; through the mode, the efficient and complete nucleus pulposus acellular matrix can be obtained, and the nucleus pulposus acellular matrix hydrogel material which has good activity, proper mechanical property, quick crosslinking, injectability and strong applicability can be obtained.

The invention provides a method for verifying gene functions based on combination of agrobacterium rhizogenes and a movable element. The provided method comprises the following steps: (1) constructing an expression vector fused by a target gene and a movable element, wherein the target gene is a functional gene to be verified of the plant; (2) transferring the expression vector into agrobacterium rhizogenes to obtain transformed agrobacterium rhizogenes; (3) infecting a plant by utilizing the transformed agrobacterium rhizogenes to obtain a positive plant with hairy roots; (4) determining the function of the target gene in the plant through the expression of the target gene in the upper end tissue of the positive plant and the phenotypic difference between the target gene and the control plant; wherein the movable element is a genetic material capable of transferring the target gene from the hairy root of the positive plant to the upper tissue. The method provided by the invention is simple to operate, high in transformation efficiency and stable and reliable in phenotype, and can be widely applied to plant gene function research.

The invention relates to a breast milk cold sterilization and freeze-drying method for full-closed treatment and application thereof, and is characterized in that the method comprises the following steps: injecting breast milk into a collection bag, carrying out vacuum sealing treatment, carrying out sterilization treatment by adopting cold sterilization, and carrying out freeze-drying to obtain freeze-dried breast milk; the cold sterilization comprises the steps of putting the collection bag into a closed pressure treatment cabin, and carrying out pressure treatment twice. According to the method disclosed by the invention, cold sterilization is adopted to uniformly transfer pressure to microbial cells in breast milk by utilizing the incompressible characteristic of a food-grade hydraulic medium on the premise of not depending on thermal action and avoiding damage of heat to nutritional ingredients, and efficient sterilization is realized by destroying cell membrane structures, enzyme active sites and genetic materials of microorganisms.

This disclosure describes techniques for detecting genetic material in a sample using a fluidic reactor device. The fluidic reactor device may receive a fluid containing genetic material to be processed through a fdter component. The fluidic reactor device may react an enzyme with the fluid at a first component to expel genetic material from the filter component. The fluidic reactor device may also react another enzyme with the genetic material in order to generate extracellular genetic material, which may be amplified. CRISPR-Casl3 techniques may be performed by the fluidic reactor device on the amplified genetic material in order to generate fluorescence based on the presence of the amplified extracellular genetic material.