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998 results about "Water in oil emulsion" patented technology

Method for preparing molecular cloning chip for high-throughput cloning of nucleic acid molecule

The invention provides a method for preparing a molecular cloning chip by cloning high flux nucleic acid molecule. The method comprises the following steps: a. a cloning nucleic acid molecule template is mixed with a nucleic acid amplification reaction system reagent to obtain an amplification mixture solution; b. the amplification mixture solution is added with a high molecular compound; c. the solution is added with oil phase, of which the volume is two times of the volume of the solution for emulsification to obtain water-in-oil emulsion; d. the emulsion is placed in a PCR meter or a thermostat to undergo a nucleic acid amplification reaction; e. after the nucleic acid amplification reaction, the emulsion is cooled down to form nucleic acid molecule cloning grains; f. the nucleic acid molecule cloning grains are separated; and g. the nucleic acid molecule cloning grains are randomly dispersed on a solid phase carrier to fix the nucleic acid amplification products in the solution on the solid phase carrier and form a nucleic acid molecule cloning array on the surface of the solid phase carrier and thus the cloning chip is obtained. The method has the advantages of improving preparation sequencing template, ensuring high flux of sequencing and lowering sequencing cost.
Owner:SOUTHEAST UNIV
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