793 results about "Polymerase chain reaction" patented technology

The invention discloses a tryptophan synthase mutant as well as a coding gene, a vector, recombinant bacteria and application thereof. Comprising the following steps: carrying out error-prone PCR (Polymerase Chain Reaction) on a coding gene trpBA of TrpS from EscherichiaoliK12MG1655, screening mutants by combining high-throughput screening, and finding that the affinity of the TrpS to a substrate indole can be effectively improved by mutating 114-site Gln of a beta subunit of the TrpS into Met and mutating 84-site Gly into Ser, so that the synthesis of L-tryptophan is promoted. The efficiency of the TrpS for converting indole and L-serine into L-tryptophan is higher due to the improvement of the affinity of indole. A new idea is provided for efficient production of L-tryptophan in the fermentation industry.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmL424. The molecular marker is YTU024-L10; the nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; a marker primer of the molecular marker YTU024-L10 is used for carrying out PCR (Polymerase Chain Reaction) amplification on wheat genome DNA (Deoxyribose Nucleic Acid) to be detected to obtain a corresponding amplification product with the molecular weight of 238 bp, namely the molecular marker closely linked with the wheat powdery mildew resistance gene PmL424. The molecular marker provided by the invention can more accurately and efficiently detect a genetic mapping population of PmL424, and map-based cloning and fine positioning of PmL424 are facilitated; and secondly, the marker is used for molecular marker-assisted selection of PmL424, so that the wheat breeding cycle can be shortened, and the breeding efficiency can be improved.

The invention discloses a primer group and a kit for simultaneously detecting nucleic acids of various respiratory pathogens based on twenty-six PCR (Polymerase Chain Reaction). The kit comprises primer pool freeze-dried powder of influenza A virus (FluA), influenza B virus (FluB), respiratory syncytial virus (RSV), human parainfluenza virus (hPIV), mycoplasma pneumoniae (MP), novel coronavirus (SARS-CoV-2), human respiratory adenovirus (HADV), human respiratory nose / enterovirus (HRV / HEV), bordetella pertussis (BP), human coronavirus (HCoV), human bocavirus (HBoV) and chlamydia pneumoniae (CP), PCR (polymerase chain reaction) freeze-dried powder and a buffer solution. According to the kit, primer system optimization is carried out on various pathogens, brand new primers and probe pools are designed, clinical sensitivity and specificity can be achieved, and meanwhile, the kit is matched with a fully-closed full-automatic detection card box integrating sample treatment and amplification and is suitable for rapid detection of respiratory tract pathogens.

The invention discloses a verification method for regulating and controlling proliferation and differentiation of bovine adipocytes by gene interference, which comprises the following steps: cutting bovine adipocyte tissues into small blocks, directly pasting the small blocks in a culture dish for culture, and promoting cell growth and proliferation by using nutrition and growth factors of the tissues; a ZNF423 gene is targeted by adopting an siRNA technology, siRNA is transfected into bovine fat cells by utilizing a transfection reagent, and the siRNA is an interference fragment containing si-ZNF423; the method comprises the following steps: respectively extracting total RNA (Ribonucleic Acid) and total protein of bovine adipocytes, and detecting by utilizing qPCR (Quantitative Polymerase Chain Reaction) quantitative analysis, a WB (White Batch) technology, EdU dyeing, flow cytometry detection, oil red O, BODIPY (Boron Dipyrromethene), Nile Red dyeing, TG (Triglyceride) content determination and the like. According to the invention, ZNF423 gene expression is interfered by the interference fragment containing si-ZNF423, and the effect of inhibiting proliferation and differentiation of bovine adipocytes is detected by various methods, so that the effect of the interference fragment in the bovine adipocytes is known conveniently, and regulation and control of proliferation and differentiation of the bovine adipocytes are realized by interfering gene expression by the interference fragment. The beef quality can be improved; the beef cattle breeding can be optimized.

The invention discloses application of iron ruthenium nano-enzyme in preparation of nucleic acid detection products, and belongs to the technical field of biological detection and nano-materials. The RIN has stable peroxidase-like (POD) activity, and can catalyze a chromogenic reaction between 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) and hydrogen peroxide (H2O2). The catalytic activity of the nano-enzyme can be selectively inhibited by pyrophosphate (PPi) generated in a nucleic acid amplification reaction, so that visual colorimetric analysis on whether target nucleic acid exists or not is realized. In combination with a polymerase chain reaction (PCR) system, the invention constructs a new nucleic acid detection strategy which does not need fluorescence labeling, is simple and convenient to operate and has high sensitivity. Through verification, the system can be widely applied to rapid on-site screening and visual interpretation of related nucleic acid of drug-resistant pathogens (such as ESKAPE flora) and the like, and has a good application prospect.

The invention discloses a construction method and application of an NMD escape model of an Alport syndrome mouse. A non-human animal model carrying Col4a5 gene c.4432delG frame shift mutation is prepared on the basis of a CRISPR / Cas9 gene editing technology. The method comprises the following steps: co-injecting gRNA of a 49 exon of a targeted Col4a5 gene, homologous recombinant donor oligonucleotide containing c.4432delG mutation and Cas9 nuclease into a mouse fertilized egg, and carrying out embryo transplantation to obtain an F0-generation mutant mouse; a mutation site is verified by combining PCR (Polymerase Chain Reaction) with sequencing, and a stably inherited mutation line is established through two generations of breeding. Through verification, the model accords with pathological characteristics of the Alport syndrome, can stably simulate typical clinical manifestation and pathological characteristics of the human X-linked Alport syndrome, and can be used as an important tool for research of the Alport syndrome.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (Polymerase Chain Reaction) kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.21. The invention also discloses a kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8. According to the invention, after a single system of an African horse pestivirus VP7 gene, an African horse pestivirus-NS2 gene, a west nile virus-NS5 gene, a west nile virus-NS2a gene, an equine influenza H3N8-HA gene and an equine influenza H3N8-NA gene is tested, a seven-fold digital PCR (Polymerase Chain Reaction) system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The invention discloses a chitinase mutant obtained based on random mutation as well as a preparation method and application of the chitinase mutant. On the basis of an error-prone PCR (Polymerase Chain Reaction) technology, the mutant ChiA-7 (T165I / C253Y / A391T / N480S / T508S / K547T / I550V / N607T) of the chitinase ChiA of the bacillus thuringiensis is obtained by the invention. Compared with ChiA, the ChiA-7 has better stability, and the enzyme activity of the ChiA-7 is about 90% of the initial enzyme activity after the ChiA-7 is subjected to heat preservation for 3 hours at 40 DEG C in a phosphate buffer solution with the optimal catalysis condition of 40 DEG C and the pH value of 4.0, while the enzyme activity of the ChiA-7 is attenuated to 75% of the initial enzyme activity. The chiA-7 is connected with a carrier pET20b (+) and is transferred into escherichia coli Escherichia coli BL21 (DE3), and the extracellular enzyme activity of a recombinant bacterium reaches 10.23 U / mL, which is improved by 13.41% compared with the enzyme activity of a control bacterium for expressing chiA. The chitinase mutant obtained by the preparation method disclosed by the invention has good thermal stability and enzymatic activity, and can be applied to hydrolysis of chitin and inhibition of mould.

The present disclosure provides compositions, apparatus and methods for conducting separate sequencing batches on a support having nucleic acid template molecules immobilized thereon, where the separate sequencing batches can be conducted using any massively parallel sequencing technology. In some embodiments, a plurality of sub-populations of nucleic acid template molecules are immobilized to the support including at least a first and second sub-population. In some embodiments, the first sub-population of template molecules undergo first batch sequencing reactions and a region of the support is imaged to detect the first sequencing reactions, wherein the second sub-population of template molecules do not undergo sequencing reactions. In some embodiments, the second sub-population of template molecules undergo second batch sequencing and the same region of the support is imaged to detect the second sequencing reactions, wherein the first sub-population of template molecules do not undergo sequencing reactions.

The invention provides a diagnostic marker for systemic lupus erythematosus and application of the diagnostic marker, and belongs to the technical field of biological medicine. The diagnostic marker is at least one protein of PDGFA (Platelet Derivative Growth Factor Amplification), APOD (Adenosine Polymerase Chain Reaction), CFHR5, AMDHD1 and SCGB3A1; the amino acid sequence of the PDGFA is as shown in SED ID NO. 1; the amino acid sequence of the APOD is as shown in SED ID NO. 2; the amino acid sequence of the CFHR5 is as shown in SED ID NO.3; the amino acid sequence of AMDHD1 is as shown in SED ID NO. 4; and the amino acid sequence of the SCGB3A1 is as shown in SED ID (Sequence of Identification) NO.5. According to the present invention, the five proteins are adopted as the SLE disease diagnosis biomarker independently or in the combination manner, such that advantages of high accuracy, high sensitivity and high specificity are provided, and the new target spot is provided for the diagnosis, the intervention improvement and the like of the SLE patient.

The invention discloses an InDel molecular marker related to wheat yield traits and application of the InDel molecular marker, and belongs to the technical field of molecular genetics. The InDel molecular marker is a molecular marker InDel 509; the nucleotide sequence of the molecular marker InDel 509 is as shown in SEQ ID No. 1. A wheat TaPRR95-5A gene functional marker InDel 509 is used for carrying out PCR (Polymerase Chain Reaction) amplification, polyacrylamide gel electrophoresis detection is carried out, and a germplasm material carrying a Hap II type is screened out according to the size of a target band. The functional marker can provide an effective marker resource for wheat genetic improvement and molecular marker-assisted breeding.

The invention belongs to the field of composite materials, and discloses a nano injection molding polyethylene glycol terephthalate composite material capable of being used for aluminum alloy and application of the nano injection molding polyethylene glycol terephthalate composite material. The PET composite material comprises the following components in percentage by weight: 23-83% of PCR PET (Polymerase Chain Reaction Polyethylene Terephthalate); 3%-7% of PBT; 3%-10% of polyarylsulfone; 0.8%-10% of a reactive toughening agent; 0-1% of an antioxidant; 0-1% of a release agent; and 10%-50% of glass fiber. The PET composite material provided by the invention can realize high PCR content, has high aluminum alloy binding force, and is smooth in demolding and stable in process. The problem that the existing nano injection molding material cannot realize high PCR content is solved.

The invention provides a single-molecule gene sequencing method based on an MOSFET device, and belongs to the technical field of single-molecule gene sequencing. A grid electrode and a source and drain electrode area of the MOSFET device are coated with a chromium surface and a gold surface in sequence, after the grid electrode is coated with a PMMA coating, a hole only capable of containing single polymerase is developed through high-precision electron beam exposure, and the single polymerase is formed in the hole; the PDMS micro-channel is attached to the periphery of the grid electrode to form a micro reaction chamber, and electronegative polymerase is driven into a high-potential PMMA hole by utilizing a negative pressure circulating scanning method and forms gold-sulfur bond connection with a bottom grid electrode gold atom, so that modification of a single polymerase on the grid electrode is completed. By further upgrading and improving a product-grade MOSFET device, a sequencing technology which takes a single polymerase chain reaction as a sequence information feedback source and realizes high-precision reading of a sequence by utilizing an intrinsic physiological function of biomolecules is formed, and an effective signal analysis method is constructed, so that the information reading effectiveness is improved, and the accuracy of information reading is improved. The accuracy and the high efficiency of a single molecule sequencing technology are improved.

The invention discloses a method for producing 5-aminolevulinic acid by inhibiting expression of a global transcriptional regulatory factor gene mcbR in corynebacterium glutamicum. The method comprises the following steps: (1) constructing a pCRISPRi-mid-McbR plasmid and a pCRISPRi-high-McbR plasmid; (2) respectively introducing the plasmids into corynebacterium glutamicum Cgh3 through electrotransfection, verifying by using a bacterial colony PCR (Polymerase Chain Reaction) mode to obtain an expression strain Cgh3-m-McbR or Cgh3-h-McbR for inhibiting a global transcriptional regulation factor gene mcbR in the corynebacterium glutamicum, and fermenting to obtain 5-aminolevulinic acid; experiments prove that compared with a control strain Cgh3-pXi, the strain for inhibiting the expression of the global transcriptional regulation factor gene mcbR in corynebacterium glutamicum has the advantage that the yield of 5-aminolevulinic acid is increased by 1.27 times or more after fermentation.

The invention relates to the technical field of medical instruments, in particular to a sample collecting device for PCR detection, which comprises a sampling tube, a sampling mechanism and a protective cover, the sampling tube comprises a tube body, and the bottom of the tube body is provided with an opening; the sampling mechanism comprises a sampling rod, the sampling rod is located in the pipe body, sampling cotton is arranged at the end of the sampling rod, a driving handle is fixedly connected to the upper end of the sampling rod, the pipe body is sleeved with the driving handle, and the driving handle is in threaded fit with the pipe body. The protective cover comprises a shell, the shell is arranged at the bottom of the pipe body in a sleeving mode, and a protective opening is formed in the bottom of the shell. The protective cover further comprises a sliding plate, the sliding plate can horizontally slide in the shell, and a connecting piece is arranged between the sliding plate and the pipe body. A driving plate is rotationally arranged on the driving handle and can be inserted into the shell and drive the sliding plate to move in the horizontal direction, the protective opening is opened while the oral cavity of the human body is opened, meanwhile, the connecting piece can be cut off, and separation of the protective cover and the sampling tube is achieved.

The invention provides a 2-succinyl-5-enol acetonyl-6-hydroxy-3-cyclohexene-1-carboxylic acid (SEPHCHC) synthase mutant, which is characterized in that on the basis of a bacillus subtilis SEPHCHC synthase gene MenD, 12 SEPHCHC synthase mutants are obtained by virtue of a site-specific mutagenesis PCR (Polymerase Chain Reaction) technology, and the SEPHCHC synthase gene MenD is used as a base. Recombinant bacteria of bacillus subtilis or bacillus natto or bacillus licheniformis or bacillus amyloliquefaciens for expressing the mutant are respectively constructed, and the yield of heptaene menadione fermented by the recombinant bacteria is 74%-203% of the yield of heptaene menadione fermented by the recombinant bacteria of bacillus subtilis for expressing wild type SEPHCHC synthase MenD.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmCWI16926. The molecular marker is YTU2BS-P12, and the molecular marker is a molecular marker PmCWI16926. The nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; the molecular marker is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid) to obtain a corresponding amplification product with the molecular weight According to the molecular marker YTU2BS-P12 closely linked with the wheat powdery mildew resistance gene PmCWI16926, provided by the invention, a genetic mapping group of the PmCWI16926 can be more efficiently detected, and map-based cloning of the PmCWI16926 is facilitated; when the marker is used for molecular marker-assisted selection of PmCWI16926, the breeding period can be shortened, the breeding efficiency can be improved, and the marker can be better applied to wheat breeding for disease resistance.

The invention provides a reagent and a method for enhancing the tolerance of a PCR (Polymerase Chain Reaction) inhibitor and application. The reagent comprises arginine and sodium lauroyl sarcosinate, the method comprises the step of adding arginine and sodium lauroyl sarcosinate into a reagent for PCR (Polymerase Chain Reaction) amplification. The reagent disclosed by the invention has relatively strong tolerance to interference of inhibitors such as non-target nucleic acid and the like. The method is simple to operate, low in cost and high in nucleic acid detection sensitivity. The reagent and the method have good application prospects in detection of nucleic acid extracted from samples such as whole blood, urine, sputum and the like.

The invention discloses a variable region amino acid sequence of a monoclonal antibody of a West Nile virus non-structural protein NS1 and application of the variable region amino acid sequence, and belongs to the technical field of medicines. According to the invention, West Nile virus non-structural protein NS1 expressed by human embryo kidney 293 cells is used as an antigen to immunize a rabbit, B cells capable of being specifically combined with the West Nile virus non-structural protein NS1 are screened from rabbit spleen cells through flow sorting, and a signal peptide and a variable region gene fragment of an antibody are cloned through reverse transcription-polymerase chain reaction; according to the present invention, the non-structural protein NS1 of flaviviridae flaviviridae virus is taken as a template, and is connected with a constant region gene to an expression vector, and after mammalian cell expression and purification, the monoclonal antibody which has high affinity and is not combined with the non-structural protein NS1 of other eight viruses of flaviviridae flaviviridae virus is obtained through enzyme-linked immunosorbent assay; the monoclonal antibody has application value in diagnosis and prevention and treatment of West Nile virus infection.

The invention relates to a framework based on a miR-30 framework, a construction method of the framework and application of the framework in RNAi (Ribonucleic Acid Interference). The skeleton construction method comprises the following steps: taking an existing shRNAmiR30 vector as a PCR (Polymerase Chain Reaction) template; the method comprises the following steps: designing two pairs of complementary overlapping primers of siRNA containing a target gene, namely primers F1 and R1 and primers F2 and R2, respectively performing amplification, and performing OE-PCR by taking the obtained P1 and P2 as templates and the primers F1 and R2 as upstream and downstream primers to obtain miR-OE. Furthermore, according to the construction method disclosed by the invention, reverse mutation of a stem-loop structure in a miR-OE skeleton and an EcoRI restriction enzyme cutting site is also carried out. The primers required to be synthesized in the miR-OE skeleton and miR-OE-EL construction method are short chains with the length of less than 60nt, the synthesis difficulty and cost are effectively reduced, and the primer design is more flexible; moreover, the interference efficiency of the miR-OE-EL skeleton on the target gene is obviously improved, and the miR-OE-EL skeleton can be well applied to preparation of shRNA drugs.

The invention discloses an application of a rice OsRNU6 gene. The login number of the nucleotide sequence of the OsRNU6 gene in Genbank is LOC4332833, and the login number of the nucleotide sequence of the OsRNU6 gene in Genbank is LOC4332833. RNA-seq is utilized to discover that the OsRNU6 shows response to the external nitrogen supply level, and fluorescent quantitative PCR (Polymerase Chain Reaction) proves that the OsRNU6 is induced by low nitrogen. The OsRNU6 is knocked out, so that the nitrogen absorption rate, plant height, tillering and yield of the rice are reduced, and the comprehensive performance is that the utilization efficiency of a nitrogen fertilizer is reduced. The function of the OsRNU6 gene for positively regulating the utilization efficiency of the rice nitrogen fertilizer is defined, and a technical route for improving the utilization efficiency of the rice nitrogen fertilizer by increasing the expression of the OsRNU6 in rice is provided.

The invention relates to the technical field of biology, and particularly discloses a kit for detecting haemophilus influenzae based on PCR (Polymerase Chain Reaction) / RAA-CRISPR-Cas13a. The invention discloses a kit for detecting a haemophilus influenzae gene on the basis of PCR (Polymerase Chain Reaction) / RAA-CRISPR-Cas13a. The kit comprises a primer pair for specifically amplifying haemophilus influenzae genes and crRNA, the sequence of the crRNA is composed of an anchoring sequence used for being combined with Cas13a protein and a guide sequence of a target sequence of the haemophilus influenzae genes, and the guide sequence is shown as the 39-66th site of SEQ ID No.28. The invention constructs a method for detecting haemophilus influenzae genes by combining a PCR or RAA technology with a CRISPR-Cas13a technology, the method is simple, convenient and rapid, has higher sensitivity and specificity, can reach a single copy level in sensitivity, and can be used for clinically and rapidly identifying whether the haemophilus influenzae is infected or not.

The invention discloses a method for constructing an Asiatic corn borer GBP gene homozygous mutant, which comprises the following steps: amplifying and cloning an Asiatic corn borer GBP gene sequence by using RACE technology, and the nucleotide sequence of the Asiatic corn borer GBP gene is as shown in SEQ ID No.1; two sgRNA target sequences are designed on a second exon of a GBP gene sequence of the ostrinia furnacalis, target sgRNA is synthesized through in-vitro transcription, the target sgRNA and Cas9 protein are fused in vitro to form an RNP mixture, and the RNP mixture is injected into ovum of the ostrinia furnacalis; the method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification on a target spot by using a target spot detection primer, sequencing to confirm a mutant individual, hybridizing and selfing to obtain a GBP homozygous mutant population. The Asiatic corn borer GBP gene homozygous mutant is constructed, so that the reproduction and immune functions of the mutant are remarkably reduced, and reference and research basis are provided for later development and formation of efficient pest biopesticide.

The invention discloses a larimichthys crocea IL-22 recombinant protein which is a larimichthys crocea IL-22 mature peptide sequence, and the amino acid sequence of the larimichthys crocea IL-22 recombinant protein is as shown in SEQ ID NO. 2. The recombinant protein is obtained by amplifying and coding gene segments of amino acids from 31st to 190th positions of large yellow croaker IL-22 mature peptide through PCR (Polymerase Chain Reaction), and transforming escherichia coli for heterologous expression. The larimichthys crocea IL-22 recombinant protein can activate a transcription factor STAT3 in a larimichthys crocea head kidney cell line (LYCK) and also can induce expression of inflammatory factors (IL-1beta, IL-6 and IL-8) in immune organs of larimichthys crocea, so that the larimichthys crocea IL-22 recombinant protein has an obvious immunoregulation effect and shows a good application prospect in research and development of aquatic immunomodulators.

The invention discloses a multiple qPCR (quantitative polymerase chain reaction) primer probe group for drug-resistant gene mutation detection of eleusine indica and application of the multiple qPCR primer probe group. The multiple qPCR primer probe group comprises ALS-F, ALS-R, ALS-P, GS1-1-GF2, GS1-1-GB2, GS1-1-R, GS1-1-P, EPSP-F, EPSP-R, EPSP-P2, 102I-T, 102-B, 106-P and 106-B. The multiple qPCR primer probe group is used for detecting drug-resistant gene mutation of eleusine indica. The invention also discloses a kit comprising the primer probe group and application of the primer probe group and the kit in detection of drug-resistant gene mutation of eleusine indica. The method has the advantages of high throughput, simplicity and convenience in operation, visual result, more time saving and the like.

The invention provides a qPCR (quantitative polymerase chain reaction) method for detecting baculovirus plasmid recombination rate. The qPCR method comprises the following steps: designing a total baculovirus primer; designing a recombinant stem grain primer; the qPCR detection method comprises the following steps: adopting a fluorescent dye qPCR method, taking the extracted stem grain DNA as a template, respectively detecting the same template by using the total stem grain primer pair and the recombinant stem grain primer pair to obtain the Ct total value of the total stem grain and the Ct weight value of the recombinant stem grain, obtaining the difference value delta Ct = Ct total-Ct weight, and calculating the recombination ratio% = 2 delta Ct * 100%. The invention provides a novel method for detecting the proportion of recombinant rods, the total rod primer pairs Bacmid-F and Bacmid-R can specifically amplify non-recombinant rods and recombinant rods, and the recombinant rod primer pairs Tn7L-F and M13-R can only amplify a sample containing the recombinant rods, so that the proportion of the recombinant rods in the sample can be detected by adopting a qPCR (quantitative polymerase chain reaction) method; the recombination proportion can be used for guiding subsequent experiments.

The invention discloses a primer group and a kit for high-throughput sequencing of targeted genital tract infection pathogens and application of the primer group and the kit, microorganisms and related drug-resistant genes in a detection range are identified by combining multiple PCR (polymerase chain reaction) with high-throughput sequencing, and the sensitivity is high. The detection technology is applied to identification of genital tract infection pathogens and has important clinical significance, the operation steps are simplified, the detection cost is reduced, and the detection efficiency is improved.

The invention belongs to the technical field of genetic engineering, and relates to the field of wheat genetic engineering, in particular to a gene TaABCG42 for regulating and controlling cadmium accumulation of wheat as well as an encoding protein and application of the gene TaABCG42. According to the invention, a TaABCG42 gene is knocked out by using CRISPR / Cas9, a TaABCG42 gene overexpression plant is constructed at the same time, and a homozygous mutant is obtained by using a Hi-Tom sequencing technology and PCR (Polymerase Chain Reaction) successive generation screening. A wild type TaABCG42 homozygous overexpression and knockout plant treated with a 40 [mu] mol / L Cd solution for 5 days is subjected to analysis such as metal content determination, subcellular component metal determination, Cd chemical form determination and Cd ion flow determination, and the Cd absorption and transport approach of TaABCG42 is analyzed, so that the physiological and molecular mechanism of the TaABCG42 metal transporter for absorbing and transporting Cd is explored, and the application prospect is wide. The understanding on the wheat ABC metal transporter is enriched, and a theoretical basis is provided for cultivating high-quality wheat with low Cd accumulation.

The utility model discloses a detachable PCR (Polymerase Chain Reaction) reaction tube bracket, aiming at solving the problems of complicated operation, low efficiency and insufficient flexibility of the PCR reaction tube bracket in the prior art. The support comprises a plurality of eight-connecting-pipe supports, a vertical supporting strip seat and a transverse supporting seat, and the eight-connecting-pipe supports are fixedly connected with one another through a plurality of notches and easy-to-break connecting points to form an eight-connecting-pipe support set. According to the utility model, the bracket can be used for full-page computer experiment, and can also be disassembled into 1-12 eight-connecting-pipe brackets which are flexibly combined for use, so that the convenience and the efficiency of experiment operation are remarkably improved. Through the detachable design, a PCR experiment can be more efficiently carried out in a laboratory, different experiment requirements are met, and resource waste is avoided.