548 results about "Polymerase chain reaction" patented technology

The invention discloses application of iron ruthenium nano-enzyme in preparation of nucleic acid detection products, and belongs to the technical field of biological detection and nano-materials. The RIN has stable peroxidase-like (POD) activity, and can catalyze a chromogenic reaction between 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) and hydrogen peroxide (H2O2). The catalytic activity of the nano-enzyme can be selectively inhibited by pyrophosphate (PPi) generated in a nucleic acid amplification reaction, so that visual colorimetric analysis on whether target nucleic acid exists or not is realized. In combination with a polymerase chain reaction (PCR) system, the invention constructs a new nucleic acid detection strategy which does not need fluorescence labeling, is simple and convenient to operate and has high sensitivity. Through verification, the system can be widely applied to rapid on-site screening and visual interpretation of related nucleic acid of drug-resistant pathogens (such as ESKAPE flora) and the like, and has a good application prospect.

The invention discloses a construction method and application of an NMD escape model of an Alport syndrome mouse. A non-human animal model carrying Col4a5 gene c.4432delG frame shift mutation is prepared on the basis of a CRISPR / Cas9 gene editing technology. The method comprises the following steps: co-injecting gRNA of a 49 exon of a targeted Col4a5 gene, homologous recombinant donor oligonucleotide containing c.4432delG mutation and Cas9 nuclease into a mouse fertilized egg, and carrying out embryo transplantation to obtain an F0-generation mutant mouse; a mutation site is verified by combining PCR (Polymerase Chain Reaction) with sequencing, and a stably inherited mutation line is established through two generations of breeding. Through verification, the model accords with pathological characteristics of the Alport syndrome, can stably simulate typical clinical manifestation and pathological characteristics of the human X-linked Alport syndrome, and can be used as an important tool for research of the Alport syndrome.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (Polymerase Chain Reaction) kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.21. The invention also discloses a kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8. According to the invention, after a single system of an African horse pestivirus VP7 gene, an African horse pestivirus-NS2 gene, a west nile virus-NS5 gene, a west nile virus-NS2a gene, an equine influenza H3N8-HA gene and an equine influenza H3N8-NA gene is tested, a seven-fold digital PCR (Polymerase Chain Reaction) system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The invention discloses a chitinase mutant obtained based on random mutation as well as a preparation method and application of the chitinase mutant. On the basis of an error-prone PCR (Polymerase Chain Reaction) technology, the mutant ChiA-7 (T165I / C253Y / A391T / N480S / T508S / K547T / I550V / N607T) of the chitinase ChiA of the bacillus thuringiensis is obtained by the invention. Compared with ChiA, the ChiA-7 has better stability, and the enzyme activity of the ChiA-7 is about 90% of the initial enzyme activity after the ChiA-7 is subjected to heat preservation for 3 hours at 40 DEG C in a phosphate buffer solution with the optimal catalysis condition of 40 DEG C and the pH value of 4.0, while the enzyme activity of the ChiA-7 is attenuated to 75% of the initial enzyme activity. The chiA-7 is connected with a carrier pET20b (+) and is transferred into escherichia coli Escherichia coli BL21 (DE3), and the extracellular enzyme activity of a recombinant bacterium reaches 10.23 U / mL, which is improved by 13.41% compared with the enzyme activity of a control bacterium for expressing chiA. The chitinase mutant obtained by the preparation method disclosed by the invention has good thermal stability and enzymatic activity, and can be applied to hydrolysis of chitin and inhibition of mould.

The present disclosure provides compositions, apparatus and methods for conducting separate sequencing batches on a support having nucleic acid template molecules immobilized thereon, where the separate sequencing batches can be conducted using any massively parallel sequencing technology. In some embodiments, a plurality of sub-populations of nucleic acid template molecules are immobilized to the support including at least a first and second sub-population. In some embodiments, the first sub-population of template molecules undergo first batch sequencing reactions and a region of the support is imaged to detect the first sequencing reactions, wherein the second sub-population of template molecules do not undergo sequencing reactions. In some embodiments, the second sub-population of template molecules undergo second batch sequencing and the same region of the support is imaged to detect the second sequencing reactions, wherein the first sub-population of template molecules do not undergo sequencing reactions.

The invention provides a diagnostic marker for systemic lupus erythematosus and application of the diagnostic marker, and belongs to the technical field of biological medicine. The diagnostic marker is at least one protein of PDGFA (Platelet Derivative Growth Factor Amplification), APOD (Adenosine Polymerase Chain Reaction), CFHR5, AMDHD1 and SCGB3A1; the amino acid sequence of the PDGFA is as shown in SED ID NO. 1; the amino acid sequence of the APOD is as shown in SED ID NO. 2; the amino acid sequence of the CFHR5 is as shown in SED ID NO.3; the amino acid sequence of AMDHD1 is as shown in SED ID NO. 4; and the amino acid sequence of the SCGB3A1 is as shown in SED ID (Sequence of Identification) NO.5. According to the present invention, the five proteins are adopted as the SLE disease diagnosis biomarker independently or in the combination manner, such that advantages of high accuracy, high sensitivity and high specificity are provided, and the new target spot is provided for the diagnosis, the intervention improvement and the like of the SLE patient.

The invention provides a 2-succinyl-5-enol acetonyl-6-hydroxy-3-cyclohexene-1-carboxylic acid (SEPHCHC) synthase mutant, which is characterized in that on the basis of a bacillus subtilis SEPHCHC synthase gene MenD, 12 SEPHCHC synthase mutants are obtained by virtue of a site-specific mutagenesis PCR (Polymerase Chain Reaction) technology, and the SEPHCHC synthase gene MenD is used as a base. Recombinant bacteria of bacillus subtilis or bacillus natto or bacillus licheniformis or bacillus amyloliquefaciens for expressing the mutant are respectively constructed, and the yield of heptaene menadione fermented by the recombinant bacteria is 74%-203% of the yield of heptaene menadione fermented by the recombinant bacteria of bacillus subtilis for expressing wild type SEPHCHC synthase MenD.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmCWI16926. The molecular marker is YTU2BS-P12, and the molecular marker is a molecular marker PmCWI16926. The nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; the molecular marker is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid) to obtain a corresponding amplification product with the molecular weight According to the molecular marker YTU2BS-P12 closely linked with the wheat powdery mildew resistance gene PmCWI16926, provided by the invention, a genetic mapping group of the PmCWI16926 can be more efficiently detected, and map-based cloning of the PmCWI16926 is facilitated; when the marker is used for molecular marker-assisted selection of PmCWI16926, the breeding period can be shortened, the breeding efficiency can be improved, and the marker can be better applied to wheat breeding for disease resistance.

The invention discloses a variable region amino acid sequence of a monoclonal antibody of a West Nile virus non-structural protein NS1 and application of the variable region amino acid sequence, and belongs to the technical field of medicines. According to the invention, West Nile virus non-structural protein NS1 expressed by human embryo kidney 293 cells is used as an antigen to immunize a rabbit, B cells capable of being specifically combined with the West Nile virus non-structural protein NS1 are screened from rabbit spleen cells through flow sorting, and a signal peptide and a variable region gene fragment of an antibody are cloned through reverse transcription-polymerase chain reaction; according to the present invention, the non-structural protein NS1 of flaviviridae flaviviridae virus is taken as a template, and is connected with a constant region gene to an expression vector, and after mammalian cell expression and purification, the monoclonal antibody which has high affinity and is not combined with the non-structural protein NS1 of other eight viruses of flaviviridae flaviviridae virus is obtained through enzyme-linked immunosorbent assay; the monoclonal antibody has application value in diagnosis and prevention and treatment of West Nile virus infection.

The invention relates to the technical field of biology, and particularly discloses a kit for detecting haemophilus influenzae based on PCR (Polymerase Chain Reaction) / RAA-CRISPR-Cas13a. The invention discloses a kit for detecting a haemophilus influenzae gene on the basis of PCR (Polymerase Chain Reaction) / RAA-CRISPR-Cas13a. The kit comprises a primer pair for specifically amplifying haemophilus influenzae genes and crRNA, the sequence of the crRNA is composed of an anchoring sequence used for being combined with Cas13a protein and a guide sequence of a target sequence of the haemophilus influenzae genes, and the guide sequence is shown as the 39-66th site of SEQ ID No.28. The invention constructs a method for detecting haemophilus influenzae genes by combining a PCR or RAA technology with a CRISPR-Cas13a technology, the method is simple, convenient and rapid, has higher sensitivity and specificity, can reach a single copy level in sensitivity, and can be used for clinically and rapidly identifying whether the haemophilus influenzae is infected or not.

The invention belongs to the technical field of genetic engineering, and relates to the field of wheat genetic engineering, in particular to a gene TaABCG42 for regulating and controlling cadmium accumulation of wheat as well as an encoding protein and application of the gene TaABCG42. According to the invention, a TaABCG42 gene is knocked out by using CRISPR / Cas9, a TaABCG42 gene overexpression plant is constructed at the same time, and a homozygous mutant is obtained by using a Hi-Tom sequencing technology and PCR (Polymerase Chain Reaction) successive generation screening. A wild type TaABCG42 homozygous overexpression and knockout plant treated with a 40 [mu] mol / L Cd solution for 5 days is subjected to analysis such as metal content determination, subcellular component metal determination, Cd chemical form determination and Cd ion flow determination, and the Cd absorption and transport approach of TaABCG42 is analyzed, so that the physiological and molecular mechanism of the TaABCG42 metal transporter for absorbing and transporting Cd is explored, and the application prospect is wide. The understanding on the wheat ABC metal transporter is enriched, and a theoretical basis is provided for cultivating high-quality wheat with low Cd accumulation.

Nucleic acid error suppression is provided. In various embodiments, DNA is extracted from a collection of plasma samples. A sequence library with duplex adapters is prepared by ligating a duplex adapter having a Unique Molecule Identifier (UMI) to an end of each of a plurality of strands of the extracted DNA and amplifying the extracted DNA with a first polymerase chain reaction (PCR). A subset of the whole genome library is selected and amplified with a second PCR to increase an amount of PCR duplicates. A plurality of duplex reads is sequenced from the amplified subset aligned to a host genome and denoised based on said alignment. A variant presence is detected in at least one of the plurality of duplex reads. A signature of the variant is determined, which is compared to a collection of disease-specific variant signatures. A disease type is determined based on the comparison.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (polymerase chain reaction) kit for simultaneously detecting African swine fever virus, porcine reproductive and respiratory syndrome virus and swine fever virus, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.24. After a single system of an African swine fever virus-VP72 gene, an African swine fever virus-CD2V gene, an African swine fever virus-14L gene, a porcine reproductive and respiratory syndrome virus-RdRP gene, a porcine reproductive and respiratory syndrome virus-European strain, a porcine reproductive and respiratory syndrome virus-American strain, a swine fever virus-vaccine strain and a swine fever virus-wild strain is tested, a nine-fold digital PCR system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

The invention discloses a primer and a method for quantitatively monitoring the biomass of sargassum hemiphyllum based on an environmental DNA technology, and belongs to the technical field of molecular ecology. The primer probe group comprises an upstream primer, a downstream primer and a fluorescent probe which are specifically targeted to the sargassum hemiphyllum mitochondria COX1 gene, and the sequence is shown as SEQ ID NO.1-3. The kit comprises the primer probe group, a qPCR (quantitative polymerase chain reaction) premixed solution, nuclease-free water and a sargassum hemiphyllum plasmid positive control. The method comprises the following steps: collecting a water sample, enriching eDNA, performing qPCR detection by using the primer probe group after extraction and purification, and realizing qualitative detection and quantitative evaluation of sargassum hemiphyllum through a Cq value or a standard curve. The method disclosed by the invention has the advantages of high sensitivity, strong specificity, no damage to the environment and target organisms, capability of realizing large-scale rapid general survey and the like, and is suitable for early warning of gulfweed blooms, investigation of population distribution and evaluation of ecological influence.

The invention discloses a method for detecting high sensitivity of monkey pox virus by a one-pot method RPA-CRISPR based on temperature control, and the method comprises the following steps: establishing a temperature-controlled RPA and CRISPR-Cas12b combined detection method, which is called as CRATS for short, by utilizing the reaction temperature difference between RPA amplification and CRISPR-Cas12b cutting; the reaction process in a tube system is regulated and controlled by stages through a temperature switching strategy: target amplification is completed at 37 DEG C firstly, then the temperature is raised to 60 DEG C, Cas12b cutting is started, so that sequential separation of amplification and cutting reactions is realized, and finally detection is realized through a fluorescence signal released by molecular beacon breakage. The problem that Cas enzyme cutting activity interferes with the amplification process in a traditional one-pot method reaction is solved, the detection sensitivity and specificity are improved, and a new technical means is provided for on-site rapid detection of the monkey pox virus.

The invention relates to the technical field of bioengineering, in particular to a penicillin G acylase mutant, polynucleotide, an expression vector and application. The penicillin G acylase mutant is obtained by carrying out site-directed mutagenesis on a wild type penicillin G acylase gene of parent Escherichia coli, and carrying out site-directed mutagenesis on the wild type penicillin G acylase gene to obtain the penicillin G acylase mutant. Phenylalanine (F) at the 24th site of an alpha chain, proline (P) at the 383rd site of a beta chain, threonine (T) at the 384th site of the beta chain, glutamic acid (G) at the 385th site of the beta chain and serine (S) at the 386th site of the beta chain of the penicillin G acylase are introduced and mutated by a whole plasmid PCR (Polymerase Chain Reaction) technology to obtain mutants. When the penicillin G acylase mutant obtained by the invention is used for catalyzing methyl mandelate and 3-(1-methyl-1H-tetrazole-5-yl) thiomethyl-7-aminocephalosporanic acid to synthesize cefamandole, the synthesis activity is improved, and meanwhile, the side reaction rate is greatly reduced.

The invention discloses a porcine beta-defensin-3 gene core promoter as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The sequence of the core promoter is as shown in SEQ ID No.1, and the core promoter has remarkable promoter activity in porcine small intestine epithelial cells and can be regulated and controlled by nutrient substances sodium butyrate and glutamine. The invention also discloses a construction method for specifically amplifying the core promoter, which comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification by taking porcine small intestine epithelial cell DNA (Deoxyribonucleic Acid) as a template, constructing a pMD-18T-pBD-3-P recombinant plasmid by taking upstream and downstream primers as shown in SEQ No.2-SEQ No.3, and amplifying by taking the recombinant plasmid as a template to obtain a core promoter fragment. The core promoter disclosed by the invention provides an excellent experimental system for researching a transcription regulation mechanism of the porcine beta-defensin-3 gene, the nutritional response characteristic of the core promoter provides a new platform for researching nutrition-immune interaction, and the core promoter has a wide application prospect in the field of healthy breeding of livestock and poultry.

The invention discloses a protein quantitative detection method which comprises the following steps: respectively constructing a capture probe for capturing antibody coupling magnetic beads and oligonucleotides and a detection probe for detecting antibody coupling oligonucleotides through a biorthogonal chemical coupling method; then, target protein is captured by using a biorthogonal chemical coupling and magnetic bead sandwich method comprising the steps of capture probe combination, closing, washing, detection probe combination and the like, and accurate quantification of low-abundance protein in a complex sample is realized by combining a subsequent digital PCR (Polymerase Chain Reaction) technology.

The invention discloses a gene OsHAK11 molecular marker for improving the salt tolerance of rice as well as a detection and identification method and application of the gene OsHAK11 molecular marker, and belongs to the technical field of plant molecular breeding. The molecular marker is composed of three primers of 52390-WF1 (SEQ ID NO.6), 52390-MF1 (SEQ ID NO.7) and 52390-R1 (SEQ ID NO.8), and the wild type, the heterozygous type and the homozygous mutant type of the OsHAK11 gene can be quickly and accurately distinguished through PCR (Polymerase Chain Reaction) amplification and electrophoretic analysis. The invention also provides a method for carrying out genotype detection by using the marker and an application scheme of the marker for breeding a salt-tolerant rice strain in backcross breeding. The marking system has the advantages of accuracy in detection, simplicity and convenience in operation, high breeding efficiency and the like, is suitable for large-scale popularization and application, and has important significance in accelerating salt-tolerant rice variety breeding and improving saline-alkali soil rice production capacity.

The invention provides a primer group, a kit and a method for simultaneously detecting 11 microorganisms and diagnosing chronic endometritis intrauterine infection. The primer group is used for detecting the existence of 11 chronic endometritis pathogens: chlamydia trachomatis, enterococcus faecalis, escherichia coli, trichomonas vaginalis, klebsiella pneumoniae, mycoplasma hominis, neisseria gonorrhoeae, staphylococcus, streptococcus, reproductive mycoplasma and ureaplasma urealyticum. The primer group is used for performing multiple joint detection on chronic endometritis related pathogens, has high specificity and sensitivity, and is adaptive to an endometrial tissue sample. The invention provides a method for simultaneously detecting 11 microorganisms for diagnosing chronic endometritis intrauterine infection, which comprises the following steps of: collecting an endometrial sample by adopting a disposable sterile endometrial sampler, detecting 11 microorganisms in the endometrial sample by adopting a multiplex PCR (Polymerase Chain Reaction) technology, and accurately detecting the intrauterine microbial infection condition. Meanwhile, more accurate technical support is provided for clinically identifying whether pathogenic bacteria causing chronic endometritis exist or not, and clinical doctors can comprehensively consider medication guidance conveniently.

The embodiment of the invention discloses a preparation method of a kit applied to endometrial cancer microsatellite state detection. The preparation method comprises the following steps: taking a first preset volume of polymerase chain reaction amplification liquid; taking a polymerase chain reaction primer mixed solution with a second preset volume; and combining the weighed polymerase chain reaction amplification liquid and polymerase chain reaction primer mixed liquid into the kit applied to endometrial cancer microsatellite state detection. According to the embodiment, the accuracy and the stability of microsatellite state detection of the endometrial cancer can be improved, so that the kit consumed by repeatedly detecting the microsatellite state in the endometrial cancer is reduced.

The invention relates to the field of molecular diagnosis and tumor risk assessment, in particular to a marker combination and a kit for assessing the lymphatic metastasis risk of cervical cancer and application of the marker combination and the kit. The marker comprises a group of gene markers and a group of microbial markers, the gene markers comprise CARD9, MNX1AS2, MRAS, OLFML2A and RPS28, preferably, the gene markers further comprise at least one of CFL1P1 and GRASLND, and the microbial markers comprise microorganisms of the genus Dialister, the genus Catonella and the genus Campylobacter. The marker provided by the invention can be used for constructing an accurate risk assessment model, and can be used for developing a corresponding PCR (Polymerase Chain Reaction) kit, a mass spectrum or a chromatographic analysis system to realize metastasis risk prediction, curative effect assessment and new drug screening of cervical cancer patients.

The invention relates to the technical field of molecular biology, in particular to a multiplex PCR (polymerase chain reaction) molecular marker amplification system for identifying Waxy mutant genes of wheat, a product and application of the multiplex PCR molecular marker amplification system. The multiple PCR molecular marker amplification system developed by the invention has the advantages of stable amplification and good specificity, realizes simultaneous genotype identification of three waxy genes Wx-A1, Wx-B1 and Wx-D1 through one-time PCR, is simple, convenient and rapid in detection process and high in flux, can accurately and efficiently screen out waxy wheat materials containing Waxy mutant genes, and has good application prospects. The waxy wheat breeding molecular marker-assisted selection efficiency and accuracy can be improved, the breeding process is shortened, and technical support can be provided for waxy wheat breeding work.

The PCR plate according to the present invention includes: a body portion having one or more reaction wells; an insertion portion extending from the body portion, inserted into a nucleic acid extraction cartridge, and having an injection port into which a nucleic acid solution is injected; a flow path portion allowing the nucleic acid solution to flow from the injection port to the reaction wells; and a blocking portion attached to the body portion for blocking backflow of the nucleic acid solution from the reaction wells to the flow path portion side.

The invention provides a computer-implemented method and system for non-invasive and step-by-step prediction of cancer risks and a storage medium. The method comprises the following steps: (1) determining a cancer signal score based on the level of a biomarker in a blood sample of a subject; (2) comparing the cancer signal score with a preset threshold value, and determining a primary screening positive subject; (3) further performing cancer risk determination and cancer species prediction by adopting an NGS method on the basis of the primarily screened positive subjects; wherein the biomarker is selected from at least one of AFP (Alpha Fetal Protein), CA125, CA15-3, CA19-9, CA72-4, CEA (Carcino Embryonic Acid), CYFRA (Cytochrome Factor Receptor Amplification) 21-1, ProGRP (Proso-Growth Radical Polymerase Chain Reaction), SCCA (Sequence

The invention provides a tumor patient enteral nutritional complication monitoring method and system based on data processing, and relates to the technical field of enteral nutritional complication monitoring. The method comprises the following steps: acquiring a blood detection result based on a continuous time period of a target tumor patient, and generating a blood detection result of the target tumor patient in a future time period by using a variational auto-encoder according to a polymerase chain reaction detection result of the continuous time period of the target tumor patient, detecting a polymerase chain reaction detection result of the target tumor patient in a future time period; determining the probability of pathogen infection of the target tumor patient at a future preset time point by using a graph convolutional network; and sending the probability of pathogen infection of the target tumor patient at the preset time point in the future to a hospital terminal. The method can quickly and accurately predict the probability of pathogen infection of the tumor patient in the future.

The invention provides a digital PCR (Polymerase Chain Reaction) microdroplet generation device, and relates to the field of microdroplet generation. The digital PCR microdroplet generation device comprises a liquid storage bin and a microflow plate which are communicated, the microflow plate is in an annular shape, a microflow channel is arranged in the microflow plate and used for circulating PCR reaction liquid, a cavity is further formed in the microflow plate, a microgroove is communicated between the cavity and the microflow channel, inert gas is injected into the cavity, and the inert gas is intermittently discharged from the microgroove; according to the digital PCR microdroplet generation device, the shearing rhythm of PCR is controlled through intermittent exhaust of inert gas, and the microgroove and the microfluidic channel are intersected at an acute angle, so that the size uniformity of the microdroplet is improved; a gas separation layer can be formed to prevent droplet fusion; the method is low in cost, easy to obtain and high in adaptability; after the inert gas cuts the PCR reaction liquid into the micro-droplets, the inert gas can separate the micro-droplets and continuously restrain the spatial arrangement of the micro-droplets, so that the micro-droplets are prevented from being extruded mutually during flowing, the retention and fracture of the micro-droplets are reduced, the integrity of the micro-droplets is improved, and the effective recovery rate of the micro-droplets is increased.

The utility model discloses a PCR (Polymerase Chain Reaction) micro-fluidic chip, which belongs to the technical field of biological detection and comprises a chip body and a shell, the chip body is placed in the shell, a cover plate is fixedly connected outside the shell through a hinge, buffer devices used for protecting the chip body are fixedly connected in the cover plate and the shell, and the buffer devices are fixedly connected in the shell. And the shell and the cover plate are externally and fixedly connected with the same locking device for limiting the shell and the cover plate. According to the utility model, when the chip body needs to be transferred, the chip body is firstly placed in the shell, then the cover plate is fixed on the shell through the hinge, at the moment, the locking rod is pushed to slide along the sliding sleeve, the locking rod can be accurately clamped in the locking sleeve, the cover plate is ensured to be stably closed, and when the outside is impacted or vibrated, the chip body can be conveniently transferred. When the chip body is damaged, a buffer spring in the internal buffer system can apply a counter-acting force to a buffer plate, so that external force transmission is effectively slowed down, the stability of the chip body in the shell is kept, and the chip is prevented from being damaged unnecessarily.

The utility model discloses a PCR (Polymerase Chain Reaction) eight-joint pipe cover opening and closing device which comprises a bottom plate, a lower comb plate, a vertical plate, a linear guide rail, an upper comb plate mounting plate, an upper comb plate and a quick clamping device, the lower comb plate and the vertical plate are mounted on the bottom plate in a front-back manner, and a plurality of holes for placing and fixing PCR eight-joint pipe bodies are formed in the lower comb plate; the linear guide rail is vertically arranged on the vertical plate, the upper comb plate installation plate is movably arranged on the linear guide rail through a sliding block, the upper comb plate is installed on the upper comb plate installation plate and corresponds to the lower comb plate in an up-down mode, and the upper comb plate is provided with an open groove capable of clamping a PCR eight-connection pipe cover. The rapid clamping device is installed on the vertical plate through a rapid clamping device installation plate, a handle is arranged on the upper portion of the rapid clamping device, a transmission shaft is arranged on the lower portion of the rapid clamping device, and the transmission shaft of the rapid clamping device is connected with the upper comb plate installation plate through an adjusting nut. The reaction kettle is convenient to use, time-saving and labor-saving, and can avoid the problems that the reaction liquid is polluted and the tube cover is not tightly pressed.