4results about How to "Sensitive high" patented technology

The invention relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disruption activity in sewage and application. A Y-type polypeptide containing an anchoring region, a hinge region and an antifouling region is creatively designed as a structural skeleton, and the tail ends of a main chain and a branch chain are respectively subjected to directional modification to prepare the multifunctional ligand-polypeptide-dye conjugate; an optical biosensing chip, namely a ligand-polypeptide-dye conjugate functionalized biosensing chip which is high in reaction efficiency, strong in anti-pollution characteristic and capable of being stably regenerated, is constructed by adopting a covalent coupling method, and then the quantum dot-nuclear receptor fluorescent probe which is high in sensitivity and adjustable in emission spectrum is prepared. The ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is provided, rapid quantitative detection of the endocrine disruptors in sewage is achieved, and the endocrine disruptors have broad-spectrum specific recognition capacity on a certain type of endocrine disruptors.

The present application relates to a multi-band radio frequency communication signal quality dynamic optimization method and an intelligent water meter. In view of the problem that the risk of water meter communication frequency band interruption is difficult to predict in advance in complex environment, a high sensitivity MEMS accelerometer integrated with a master control chip is used to collect three-dimensional micro-vibration signals of the water meter shell through low sampling rate, combined with time-frequency joint analysis to extract vibration modal characteristics, a graph neural network model is used to associate vibration state with radio frequency channel quality index, and real-time prediction of deterioration risk probability is realized. According to the environmental dynamic disturbance factor, the threshold value is adjusted and determined, combined with the radio frequency index step deterioration monitoring, the intelligent communication frequency band switching and context caching are triggered. The newly added vibration and radio frequency data are used for model adaptive optimization. The scheme can predict the channel interruption risk in advance, effectively improve the communication stability and adaptability of the intelligent water meter.

The present application relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disrupting activity in sewage and application. The present application creatively designs a Y-shaped polypeptide containing an anchoring region, a hinge region and an anti-fouling region as a structural framework, and performs directional modification at the ends of the main chain and the branch chain to prepare a multifunctional ligand-polypeptide-dye conjugate; a covalent coupling method is used to construct a high-response-efficiency, strong-anti-pollution-characteristic and stable-regeneration optical biosensor chip, i.e. a ligand-polypeptide-dye conjugate functionalized biosensor chip, then a quantum dot-nuclear receptor fluorescence probe with high sensitivity and adjustable emission spectrum is prepared, a ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is proposed, and rapid quantitative detection of endocrine disruptors in sewage is realized, so that the present application has broad-spectrum specific recognition ability for certain endocrine disruptors.

This invention discloses a visual detection method and application of biogenic amines based on a carbon dot / quantum dot ratio fluorescent probe, belonging to the field of food safety detection technology. Addressing the problems of existing biogenic amine detection methods, such as complex operation, difficulty in rapid on-site screening, and susceptibility to interference from single fluorescent signals, this invention prepares a ratio fluorescent probe based on graphene carbon dots (G-CDs) and near-infrared quantum dots (QD700). This probe requires no chemical modification and achieves a synergistic response of G-CDs fluorescence enhancement and QD700 fluorescence quenching through the Spe-induced aggregation effect, achieving a detection limit of 0.0097 μM and a response time faster than 2 seconds. Combined with the DEIM intelligent model, it can accurately distinguish spermine, putrescine, cadaverine, histamine, and tyramine. Integrated into a paper-based smartphone platform, it enables visual identification of shrimp freshness (red for fresh, green for spoiled), showing a high correlation with the TVB-N standard method (R²=0.95), suitable for rapid on-site monitoring.