The invention discloses a
probiotic preservation
activity detection method and
system, and relates to the technical field of
probiotic activity detection. According to the method, three-positive
cell populations are screened by constructing three-dimensional feature vectors, and a
cell bank with potential activity is delineated to eliminate interference of dead cells and impurities; the suspected dormant state
probiotic population is accurately marked through a three-dimensional
scoring system, and misjudgment of the suspected dormant state probiotic
population in the initial stage is avoided; the
resuscitation function activity rate is calculated through
resuscitation culture in combination with a three-dimensional
verification model, and dormant viable
bacteria and dead
bacteria can be clearly distinguished; finally, based on a clinical probiotic value weighting
algorithm, data of initial complete functional viable
bacteria and resuscitated complete functional viable bacteria are fused to calculate an actual
activity level value, so that accurate detection of the
resuscitation capacity of the probiotics in the dormant state in the simulated physiological environment is realized, and underestimation of the overall
activity level caused by misjudgment of dead cells in the dormant state is avoided. Meanwhile, the relevance between the detection result and the clinical probiotic value is guaranteed, and the detection accuracy is improved.