The invention discloses a sealing adhesive for a cable joint box and a preparation method thereof, and belongs to the technical field of preparation of the sealing adhesive. The sealing adhesive comprises organic silicone rubber, high-molecular weight polyisobutene, medium-molecular weight polyisobutene, low-molecular weight polyisobutylene, inorganic filler, an antioxidant, carbon black as a coloring agent and a mothproofing agent. The preparation method comprises the following steps of: S1. weighing: weighing raw materials; S2. kneading: adding the raw materials for kneading; S3. blending: blending the kneaded sizing materials; S4. extruding: extruding the blended sizing materials, and then cooling and cutting to prepare the sealing adhesive for the cable joint box. The sealing adhesive disclosed by the invention has the advantages of excellent binding property, filling property, sealing property, water resistance, high-low temperature resistance, acid resistance, alkali resistance, salt resistance and dielectric property. The preparation method disclosed by the invention has the characteristics of health, environmental friendliness, simple production process, no solvent, time and energy conservation, low cost and suitability for industrial large-scale production.