412 results about "Protein solution" patented technology

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

A preparation method of the blended modified antifibrillated lyocell fiber comprises three steps as follows: S1, a fibroin raw material and a cellulose raw material are dissolved in different NMMO / water mixed solvents respectively, and a fibroin solution and a cellulose solution are obtained; s2, transferring the fibroin solution and the cellulose solution into a reaction kettle, adding a modifier, vacuumizing to remove oxygen, heating to 50-85 DEG C, and stirring to react to obtain a composite spinning solution; the modifier is a small molecular compound containing an epoxy group or an oxygen-containing heterocyclic ring; s3, the composite spinning solution is extruded through a spinneret plate, fiber forming is conducted through a coagulating bath, drafting and winding are conducted, and the blending modified antifibrillated lyocell fiber is prepared. According to the invention, through in-situ modification of the lyocell fiber spinning solution, the prepared lyocell fiber has the intrinsic antifibrillation capability, the addition of an after-finishing process for treatment is not needed, meanwhile, the mechanical property of the fiber is improved, and the fiber is endowed with better skin-friendly property and wearing comfort.

The present invention discloses a method for improving the properties of composite meat protein gel based on modified pea protein, which belongs to the technical field of food protein processing. The present invention fully dissolves natural pea protein or pH-shifted modified pea protein to obtain a protein solution; then adds a disulfide bond cleaving agent to the protein solution, dissolves and mixes the solution to obtain modified pea protein. The present invention is based on the disulfide bond cleavage treatment of pea protein to dissociate acidic and basic subunits and expose more internal groups, thereby achieving the improvement of the solubility and functionality of pea protein itself. In addition, both natural pea protein and modified pea protein with disulfide bond cleavage can enhance the structural properties of low-salt meat protein gel under the action of cross-linking enzymes, develop low-salt meat protein gel with good gelation and network structure, and provide solutions and technical support for the quality deterioration of meat products under various circumstances.

The invention discloses a sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity and a preparation method and application thereof, belongs to the technical field of functional protein peptides, and particularly relates to a preparation method of the sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity, which comprises the following steps: heating a sea cucumber flower protein solution; the preparation method comprises the following steps: mixing sea cucumber with simulated gastric juice, reacting for 30-150 minutes, then adding simulated intestinal juice, reacting for 20-120 minutes, carrying out enzyme deactivation treatment, centrifuging, taking supernate, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; and mixing the sea cucumber flower self-assembled polypeptide with deionized water to prepare a sea cucumber flower self-assembled polypeptide solution, mixing the sea cucumber flower self-assembled polypeptide solution with a sodium selenite solution and a phosphate buffer solution, reacting for 20-60 minutes, washing with an ethanol solution, centrifugally collecting precipitate, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-selenium nano-carrier. The sea cucumber flower self-assembled polypeptide-selenium nano-carrier prepared by the invention has good antioxidant activity and potential of effectively delivering selenium to an organism.

The present invention discloses a livestock and poultry manure biochar-based fertilizer, comprising the following raw materials in parts by weight: 10-42% of dilute protein solution and collagenase-modified livestock and poultry manure biochar, 20-40% of urea, 7-25% of diammonium phosphate, 7-24% of potassium chloride or potassium sulfate, 0.5-1% of modified starch, and 6-23% of bentonite. The livestock and poultry manure is selected from one of chicken manure, cow manure, and pig manure. After the biochar is modified with collagenase, the surface of the biochar has more phenolic hydroxyl groups, carboxyl groups, aldehyde groups, and hydroxyl functional groups, which are effective for NH4 + , PO4 3‑ , K + , has a stronger adsorption effect, collagenase modification makes the protein distribution more uniform, and the generated groups form a stable chemical bond with the biochar surface: it improves the dispersibility of the modified biochar in aqueous solution and reduces agglomeration; it has better chemical stability in acidic and alkaline environments.

The invention aims to provide a method, equipment, medium and program product for determining acidity coefficient, the method comprises the following steps: constructing a simulation box corresponding to protein, the simulation box comprising the protein, a corresponding solvent and a buffer solution; performing molecular dynamics simulation on the simulation box by utilizing a trained machine learning force field to obtain corresponding track file information; and determining an acidity coefficient corresponding to each drippable site in the protein based on the track file information. A dynamic process of protein in a solution is accurately and efficiently simulated by utilizing a machine learning force field, and an accurate acidity coefficient is calculated based on a state of a protein solution system in simulation.

The invention relates to an industrial method, in particular to a method for preparing a universal functional protein nano-film on the surface of a base material, which comprises the following steps: (1) mixing a protein solution, an optional functional monomer and a photoinitiator solution to prepare a mixed solution; (2) soaking a base material in the mixed solution; and (3) irradiating with ultraviolet rays to obtain the protein nano-film on the surface of the base material. According to the universal method for continuously preparing the functional protein nano-film on the surface of the base material, rapid self-assembly aggregation and monomer polymerization of the protein can be carried out at the same time under ultraviolet induction, and the protein-functional polymer two-dimensional nano-film prepared on the surface of the base material is compact, low in roughness, high in transparency and good in application prospect. The biocompatibility is good.

The invention discloses an amyloid-like protein mediated long-acting metal organic framework antifouling agent as well as a preparation method and application thereof. Belongs to the field of marine antifouling material preparation. The anti-fouling agent is based on MOF with an anti-fouling function, and amyloid-like protein is self-assembled on the surface of the MOF to form a compact protective shell layer. According to the preparation method, metal ions and organic ligands react and are connected through coordinate bonds to form an MOF structure, and MOF particles are obtained through centrifugation, collection, precipitation, washing and drying; under the action of a water-soluble organic reducing substance, the protein completely opens a disulfide bond to form an unfolded protein chain, and the protein chain is self-assembled to obtain an amyloid-like protein solution; and placing the MOF particles in an amyloid-like protein solution, and carrying out simple one-step incubation to obtain the amyloid-like protein mediated long-acting metal organic framework antifouling agent. The antifouling agent is prepared by adopting a novel green, high-stability, rapid and efficient mild modification method, and has the advantages of stability, durability, universality and high efficiency.

The application belongs to the technical field of food processing, and particularly relates to an oat protein-pectin-proanthocyanidin compound and a preparation method thereof. The oat protein is mixed with water, and an alkali liquor is added to adjust the pH to obtain a protein material solution for medium grinding. Then, pectin is added to the ground protein material solution, and proanthocyanidin powder is added after the pH is adjusted, and secondary medium grinding is performed to obtain an oat protein-pectin-proanthocyanidin solution. Then, hydrochloric acid is slowly added to adjust the pH to 6.8-7.0. Finally, the protein solution is centrifuged, and the supernatant is spray dried to obtain the oat protein-pectin-proanthocyanidin compound. The oat protein-pectin-proanthocyanidin compound prepared by the method is a brand-new protein system, and solves the problems of poor dispersibility, nutritional defects and poor emulsifying property of oat protein, and has a wide application prospect.

The invention belongs to the field of food, and particularly relates to a preparation method of hydrolyzed whey protein. The preparation method of the hydrolyzed whey protein provided by the invention comprises the following steps: performing high-pressure homogenization on a whey protein aqueous solution, hydrolyzing the whey protein aqueous solution subjected to high-pressure homogenization in the presence of an enzyme, and performing enzyme deactivation, wherein in the step of performing high-pressure homogenization on the whey protein aqueous solution, the pressure of high-pressure homogenization is 150 to 450 MPa, and the time of high-pressure homogenization is 20 to 35 minutes. According to the method, protein feed liquid containing whey protein is subjected to high-pressure homogenization treatment before hydrolysis, so that alpha-helix, beta-fold and other protein secondary structures can be fully changed, the hydrolysis reaction time is greatly shortened, the bitter taste of the whey protein hydrolysate can be improved, and the characteristics of a whey protein hydrolysate downstream product can be improved; the osmotic pressure of the formula milk is reduced, and the reconstituability of the formula milk powder is improved.

The invention provides a magnetic bead for IgG antibody biotin labeling and purification and an application method, and the magnetic bead is prepared by the following steps: 1) preparing rProtein A and rTurbo ID into a protein solution; (2) mixing the protein solution with the carboxyl magnetic beads to react, so that the protein is coupled to the carboxyl magnetic beads to obtain rProtein A and rTurbo ID protein coated magnetic beads; according to the magnetic bead disclosed by the invention, biotin labeling of an antibody can be realized by applying Turbo ID enzyme, only ATP and common biotin molecules need to be used, and the cost is very low; and the biotin labeling reaction is carried out on the magnetic beads, so that the purification and biotin labeling operation is simplified, and the operation is simple and convenient.

The present invention discloses a preparation method of antioxidant sturgeon protein peptides, comprising the following steps: S1, extraction of crude sturgeon skin protein solution; S2, extraction of crude sturgeon swim bladder protein; S3, extraction of crude sturgeon meat protein solution; S4, mixed enzymatic hydrolysis; S5, subjecting the obtained enzymatic hydrolysis product to enzyme inactivation treatment first to obtain an enzymatic hydrolysis solution, and then sequentially ultrafiltering and vacuum drying the enzymatic hydrolysis solution to obtain antioxidant sturgeon protein peptides. The present invention mixes the proteins extracted from different parts of sturgeon in a specific ratio, and then performs enzymatic hydrolysis. The neutral protease and papain added can act synergistically to more comprehensively hydrolyze proteins, generating small peptides with specific functions. At the same time, glutathione added during enzymatic hydrolysis can protect the sulfhydryl groups of the enzyme and maintain the enzyme activity, so that the finally obtained sturgeon protein peptides have good antioxidant properties.

The application belongs to the technical field of dechlorination carrier and specifically provides a preparation method of a dechlorination active carrier, which comprises the following steps: 1) polyoxyethylene-polyoxypropylene block copolymer and modified protein solution are dissolved in deionized water, then cobalt nitrate, aluminum lactate and 2-aldehyde benzene boronic acid are added and stirred uniformly, reaction is carried out at 110-130 DEG C, centrifugation and drying are carried out to obtain a precursor; 2) tetrabutylphosphonium hydroxide and sodium aluminate are added into deionized water, TEOS and the precursor are added after mixing, stirring is carried out, crystallization is carried out at 165-175 DEG C, centrifugation and washing are carried out, and the obtained solid is dried and calcined to obtain the dechlorination active carrier. The carrier prepared by the application has the advantage of good dechlorination effect.

The invention discloses a method for extracting phycocyanin from spirulina, and belongs to the field of phycocyanin preparation. The method comprises the following steps: taking spirulina, adding water to obtain a spirulina suspension, performing freeze thawing, intermittent ultrasonic treatment and centrifugation, and collecting supernate to obtain a crude extract of phycocyanin; adding a (NH4) 2SO4 solution into the protein crude extract, standing, and centrifuging to obtain a salting-out precipitate; adding the salting-out precipitate into a mixture of ethylene glycol and inorganic salt, magnetically stirring and layering to obtain a protein solution; and carrying out ultrafiltration, centrifugation and freeze drying on the protein solution to obtain the spirulina phycocyanin. The purity of the phycocyanin can reach 1.0, the extraction rate can reach 69.87%, and the purity and the extraction rate of the phycocyanin extracted through the method are both improved. The extraction method provided by the invention has the advantages of simple process flow, low cost, high efficiency and strong practicability, and is suitable for large-scale industrial production of high-purity phycocyanin.

The invention relates to the technical field of biological engineering, in particular to a coconut protein cavitation jet-enzymolysis combined extraction device which comprises a mixing hopper, a first tank body and a second tank body, a high-pressure pump and a first pump body are installed on the first tank body and the second tank body respectively, and a protein solution and an enzyme solution are contained in the first tank body and the second tank body respectively. The second pump body guides enzyme liquid into the mixing hopper through a pipeline, an impurity removal mechanism used for purifying mixed liquid generated by the protein solution and the enzyme liquid is arranged on the outer side of the mixing hopper, a mixing mechanism used for guiding nitrogen into the mixed liquid is further arranged on the mixing hopper, multiple layers of ultrafiltration membranes are arranged in the frame body, and a transmission assembly used for shaking the ultrafiltration membranes is arranged on the frame body. A protein solution driven by the high-pressure pump and an enzyme solution conveyed by the first pump body are combined to form a cavitation jet flow effect, high-speed jet flow can generate strong shearing force and turbulent flow, protein particles are broken, contact of enzyme and a substrate is accelerated, the enzymolysis reaction rate and uniformity are remarkably improved, and the extraction time is shortened.

The invention discloses a purification method for removing endotoxin in prokaryotic expression recombinant protein, which belongs to the technical field of bioengineering, and comprises the following steps: adding 0.1% Triton X-114 in an ultrasonication stage, carrying out ice-bath ultrasonication, after the recombinant protein is combined with a Ni-NTA affinity column, washing with 50 column volumes of a combination buffer solution containing 0.1% Triton X-114, and carrying out elution by using an elution buffer solution containing 0.1% Triton X-114 in the presence of an elution buffer solution containing 0.1% Triton X-114 in the presence of an elution buffer solution containing 0.1% Triton X-114 in the presence of an elution buffer solution containing 0.1% Triton X-114; and washing with a 4-DEG C binding buffer solution with 20 column volumes, and subsequently eluting the target protein with imidazole with different gradients. Then, regulating the pH value of the purified recombinant protein solution to 2-4 or more than 10 through 100mM hydrochloric acid or sodium hydroxide, dissociating and decomposing endotoxin, carrying out oscillation treatment at room temperature for 2-4 hours, then removing the endotoxin in the recombinant protein through ultrafiltration, retaining an endotube protein solution, and detecting the content of protein and the endotoxin in the recombinant protein; the method is easy and convenient to operate and low in cost, the endotoxin content in the recombinant protein prepared through prokaryotic expression can be reduced by 400 times on the premise that immune recognition of the recombinant protein and serum is not affected, and the phenomenon that strong cross reaction occurs between immune serum and escherichia coli due to high endotoxin residues in the recombinant protein is reduced.

The invention discloses a preparation method of a white kidney bean protein-propylene glycol alginate composite carrier steady-state emulsion for enhancing alpha-amylase inhibitory activity, and belongs to the technical field of emulsion preparation. The method comprises the following steps: S1, mixing white kidney bean protein with water to prepare a protein solution; s2, mixing propylene glycol alginate with water to prepare a propylene glycol alginate solution, and then mixing the propylene glycol alginate solution with the protein solution prepared in the step S1 to prepare a water phase; s3, fat-soluble active substances are added into edible oil, and an oil phase is prepared; and S4, mixing the water phase and the oil phase, and carrying out high-speed dispersion and high-pressure homogenization to prepare the steady-state emulsion. The preparation method is simple and convenient to operate, and the prepared emulsion has good milk stability, storage stability, high active substance protection capacity and high alpha-amylase inhibitor activity.

The application discloses a preparation method of a high internal phase emulsion stabilized only by proteins, which mixes a completely hydrated protein solution as a water phase with an oil phase, and controls the hydrodynamic characteristics of a shearing emulsification process to obtain the high internal phase emulsion stabilized only by proteins. The preparation method of the application can obtain the high internal phase emulsion stabilized only by food-grade proteins through one-step shearing emulsification without other measures. The prepared high internal phase emulsion has good gel characteristics, stability and high safety, and can be used as a substitute for partially hydrogenated vegetable oil and saturated fatty acids. Meanwhile, the method is simple in operation, low in preparation cost and suitable for large-scale industrial promotion.

The invention relates to the technical field of 3D printing of easy-to-swallow food, in particular to a protein-based nanoparticle gel double-nozzle 3D printing method and equipment, aiming at the nutritional requirements of old people with dysphagia, procyanidine is loaded on protein-based nanoparticles, corn starch, gelatin and the like are combined to construct a composite gel system as a printing wall material, and the printing wall material is used as a printing wall material. Protein, citrus juice and peony seed oil emulsion are used as core materials, and a double-nozzle 3D printing technology is utilized to realize accurate texture regulation and control and personalized nutrition design; the method comprises the steps of protein solution preparation, composite nanoparticle synthesis, procyanidine loading, wall material and core material preparation, double-nozzle printing and the like, the technology breaks through the bottleneck that traditional swallowing food is insufficient in protein strengthening and lacks active ingredients, and the antioxidant functional components and the 3D printing technology are integrated; innovative food with swallowing safety and nutrition targeting is provided for the elderly, and application of protein-based materials in intelligent food manufacturing is promoted.

The invention belongs to the technical field of biological medicine, and particularly relates to a screening method of a recombinant protein for inhibiting the phagocytic function of macrophages on myelin sheath protein and a hydrogel preparation. The invention firstly provides a screening method of the recombinant protein, which comprises the following steps: co-culturing a recombinant protein solution and macrophages in an environment rich in myelin protein, and detecting various indexes to screen out the recombinant protein capable of remarkably inhibiting the phagocytic function of the macrophages on the myelin protein. The invention discloses regulation and control of the macrophage phenotypic change and the relationship between the macrophage phenotypic change and the MD phagocytosis function of the specific recombinant protein for the first time, and provides a new thought for screening the recombinant keratin with a specific function.

The invention discloses spirulina protein-zein composite nanoparticles and a preparation method and application thereof.The preparation method comprises the steps that a zein solution is added into a spirulina protein solution, ethanol in the solution is removed through evaporation, insoluble substances are removed through centrifugation, and spirulina protein-zein composite nanoparticle dispersion liquid is obtained; and freeze-drying to obtain spirulina protein-zein composite nanoparticle powder. The preparation method of the spirulina protein-zein composite nano-particles is simple, low in cost, green and safe, and the obtained nano-particles can be kept stable in a neutral pH environment, have good biocompatibility, can be applied to efficient embedding of bioactive components, and have good application prospects. The light, heat and storage stability of the bioactive components are remarkably improved; and the oxidation resistance, tyrosinase inhibition and in-vitro release performance of the bioactive components are improved.

This disclosure provides anti-VEGF antibodies formulated as high-concentration aqueous pharmaceutical compositions suitable for injection, preferably intravitreal injection. Aqueous pharmaceutical compositions are useful for delivering a high concentration of the antibody active ingredient to a patient without high levels of antibody aggregation and without a high level of sub-visible particulate matter. An aqueous composition of the disclosure comprises an antibody having a concentration of at least 50 mg / mL. An aqueous pharmaceutical composition of the disclosure includes a sugar, a buffering agent, and a surfactant.

The invention belongs to the field of preparation of Pickering emulsions, and particularly relates to a method for preparing a Pickering emulsifier from peanut protein. The preparation method of the emulsifier comprises the following steps: 1, preparing a peanut protein solution from peanut protein, and adjusting the pH value of the peanut protein solution to be neutral; step 2, heating the neutral peanut protein solution obtained in the step 1 at 90-100 DEG C; in order to further improve the stability of the Pickering emulsion in which the emulsifier participates in preparation, the preparation method of the emulsifier further comprises the following steps: step 3, heating, cooling to room temperature, and freeze-drying; carrying out superfine grinding on the freeze-dried peanut protein, redissolving and centrifuging to obtain soluble aggregate and insoluble aggregate; and 4, mixing the soluble aggregate and the insoluble aggregate according to a weight ratio of (0-1): 2. Wherein when the insoluble aggregate is completely used as the emulsifier to prepare the Pickering emulsion, the stability of the Pickering emulsion is optimal.

The invention discloses a modified cake meal protein-based degradable membrane material as well as a preparation method and application thereof. The membrane material is prepared from the following raw materials: a modifier and a cake meal protein solution, the modifier is prepared from the following raw materials: aryl diazacyclohexane and a three-arm star s-P (NIPAM-co-DMAM); the modifying agent and the cake protein form a three-dimensional cross-linked network structure through an electric field auxiliary curing technology, the prepared membrane material has excellent mechanical properties and controllable degradation characteristics, meanwhile, low-temperature efficient forming is achieved, production energy consumption is remarkably reduced, and an innovative solution is provided for high-value utilization of agricultural waste.

The application relates to the technical field of fabrics, in particular to a fabric based on protein fiber combined with natural fiber and a preparation method thereof, which is prepared by wet core-sheath composite spinning of a down protein sheath layer and a natural cellulose core layer; the core layer is natural cellulose fiber, and the total mass of the functional fabric is 70-90%; the balance is the sheath layer formed by cross-linking and solidification of a down protein solution; and the sheath layer is loaded with nano titanium dioxide; the introduced natural cellulose core layer is the main skeleton component of the fabric, selects natural fibers such as bamboo pulp cellulose and cotton linter pulp, and provides basic mechanical support for the fabric. The abandoned down resource is used to prepare protein fiber, which is combined with natural cellulose fiber to construct a composite fiber with a functional sheath layer and a mechanical core layer. The technical scheme solves the problems of insufficient sun protection, insufficient antibacterial performance and single mechanical performance of traditional natural fiber fabrics.

The application provides a high-stability salt-free meat protein beverage and a preparation method thereof, and relates to the field of food. The method comprises the following steps: extracting myofibrillar protein in chicken, washing the myofibrillar protein with water to remove salt in the myofibrillar protein, and obtaining salt-free myofibrillar protein; dissolving the salt-free myofibrillar protein in water to obtain a salt-free myofibrillar protein solution; and dissolving high-charge-density sodium carboxymethyl cellulose in the salt-free myofibrillar protein solution and uniformly mixing. The high-charge-density sodium carboxymethyl cellulose is combined with meat protein in a non-covalent form to form a complex, the charge property of the meat protein is changed, the self-assembly behavior of the myofibril is hindered, and the aggregation is inhibited, so that the water solubility of the meat protein is improved; the steric hindrance effect and the electrostatic repulsion of the system are greatly improved, so that the thermal stability of the meat protein is greatly improved, and the instability effect of the meat protein in the heat processing process is effectively avoided. The clear and transparent salt-free beverage product with good stability can be processed by using the method.

The application discloses an injectable microsphere gel of controllable performance silk protein and a preparation method thereof, and belongs to the field of biomaterials.The preparation method comprises the following steps: preparing a regenerated silk protein solution, including degumming, dissolving and purifying of mulberry silk, and obtaining a regenerated silk protein solution with a target molecular weight through molecular weight screening; preparing a silk protein microsphere aqueous solution by adding ethanol to the regenerated silk protein solution and performing freezing and thawing; obtaining a silk protein microsphere redispersible powder by drying two solutions prepared in the above steps; and preparing a silk protein microsphere gel by dispersing the silk protein microsphere redispersible powder in a solvent and adding a gel inducer.The whole preparation process does not involve toxic chemical reagents, and thus the excellent mechanical properties, ideal biocompatibility and biodegradability of the material are ensured; the silk protein microsphere gel has thixotropy, can be injected into subcutaneous tissue through a syringe, has a certain self-supporting property after injection, has controllable mechanical properties due to the molecular weight screening, and can be used in different application scenarios.

The present application mainly relates to a kind of preparation of sulfur-rich amino acid protein CMd-copper nanoclusters and its detection mercury ion method, comprising: a) by prokaryotic expression obtains CMd protein, and the protein concentration of 5 mg / mL-25 mg / mL CMd protein solution is obtained by purification.b) CMd is reacted with reducing agent TCEP after opening protein disulfide bond.c) the protein solution after reduction is reacted with anhydrous copper sulfate, and preparation high fluorescence CMd-copper nanocluster.d) CMd-copper nanocluster is applied to detect heavy metal mercury ion.The application method can be synthesized under mild aqueous conditions by "one-step method" structure controllable, stable CMd-copper nanocluster, widens the raw material of copper nanocluster synthesized with protein as template, avoids the use of organic solvent and strong reducing agent, and reduces the cost and environmental burden brought by noble metal raw material, and also has higher selectivity to mercury ion.

The invention provides a hermetia illucens protein-EGCG compound and a preparation method and application thereof, and belongs to the technical field of functional food, cosmetics or pharmaceutical preparations, and the preparation method comprises the following steps: mixing hermetia illucens larva powder with a sodium chloride solution, and stirring to extract protein; adjusting the pH value to 4.2-4.5 to precipitate protein, and cleaning and drying to obtain hermetia illucens protein powder; the method comprises the following steps: dissolving hermetia illucens protein powder in a PBS buffer solution to obtain a hermetia illucens protein solution, mixing the hermetia illucens protein solution with an EGCG solution, adjusting the pH value to 9, carrying out a stirring reaction, and carrying out dialysis and freeze-drying to obtain a hermetia illucens protein-EGCG covalent complex; and mixing the hermetia illucens protein solution with the EGCG solution, stirring for reaction, dialyzing, and freeze-drying to obtain the hermetia illucens protein-EGCG non-covalent complex. According to the invention, the EGCG is used for modifying the hermetia illucens protein, and the prepared emulsion stabilizer can improve the stability and oxidation resistance of the emulsion at the same time.

The invention discloses anthocyanin-protein composite powder with high stability as well as a preparation method and application of the anthocyanin-protein composite powder, and belongs to the technical field of biological food pigments. The preparation method comprises the following steps: mixing a black rice anthocyanin solution and a protein solution, stirring in a dark place, and freeze-drying to form the anthocyanin-protein composite powder. The anthocyanin-protein composite powder prepared by the method disclosed by the invention can be applied to the preparation of colored cold noodles, steamed buns and noodles, the stability of the black rice anthocyanin obtained by the method disclosed by the invention in tap water is improved, and the retention rate of the black rice anthocyanin in deionized water is also obviously increased; the starch is inhibited from aging to a certain extent, and the storage stability is improved. The anthocyanin in the prepared anthocyanin-protein composite powder is high in stability, the investment cost is low, and the anthocyanin-protein composite powder can be widely applied to the technical field of food processing and has wide application prospects in flour products.