531 results about "Protein solution" patented technology

The invention belongs to the technical field of biological medicines, and particularly discloses an epidermal growth factor-linker polypeptide-collagen, and the linker polypeptide has an amino acid sequence as shown in SEQ ID No.1. The linker polypeptide capable of connecting the EGF and the collagen is designed and synthesized, the N end of the linker polypeptide simulates an EGF receptor structure and can be combined with the EGF, and the linker polypeptide is connected with the EGF through non-covalent Van der Waals force, so that the structure of the EGF cannot be damaged; the C terminal of the linker polypeptide is rich in lysine residues, and can be covalently crosslinked to collagen through glutamine transaminase to form EGF-linker polypeptide-collagen. The protein solution taking EGF-linker polypeptide-collagen as active ingredients is low in cytotoxicity and high in biological safety, and meanwhile, the action time of the epidermal growth factor can be remarkably prolonged. In addition, a stable repairing microenvironment can be formed on the surface of the endometrium, and the endometrium injury repairing effect is remarkably improved.

The invention provides an easy-to-swallow nutrition-enriched plant-based protein food and a preparation method thereof, the plant-based protein food comprises a mixed protein solution and edible oil, and the mixed protein solution comprises walnut protein, soybean protein isolate, alfalfa protein and egg white protein. The preparation method of the plant-based protein food comprises the following steps: preparing a high-protein emulsion from a mixed protein solution and edible oil and fat, preparing emulsion gel from the high-protein emulsion, and performing 3D printing by adopting the emulsion gel. According to the easy-to-swallow type nutrition-enriched plant-based protein food disclosed by the invention, four proteins are compounded, the nutritional value of the food is improved through the synergistic effect, the protein content is increased, amino acid compounding is realized, and meanwhile, the rheological property and extrusion property of 3D printing after protein compounding are improved, so that the printed food can be suitable for 3D printing and also can be used for 3D printing. The food can also be applied to old people with dysphagia, and meets the requirements of daily nutrition intake and easy swallowing.

The invention belongs to the technical field of deep processing of agricultural products, and provides royal jelly protein zymolyte with melanin inhibition activity and preparation and application of the royal jelly protein zymolyte. Performing enzymolysis on the soluble royal jelly protein solution with pepsin to obtain enzymatic hydrolysate; and carrying out ultrafiltration separation on the enzymatic hydrolysate to obtain a polypeptide solution with the molecular weight of less than 3KDa, namely royal jelly protein hydrolysate. The royal jelly is used as a raw material, the royal jelly protein hydrolysate with low molecular weight is prepared by extracting royal jelly protein and utilizing a protease enzymolysis technology and an ultrafiltration separation technology, and the royal jelly protein hydrolysate has good melanogenesis inhibition activity and can reduce generation of mouse melanoma cells and zebra fish melanin. The inhibitor is derived from natural food royal jelly, is green and safe, is controllable in conditions in the preparation process, is convenient to extract, does not need artificial synthesis, and is expected to be applied to food, medicines or cosmetics with a whitening effect.

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

A preparation method of the blended modified antifibrillated lyocell fiber comprises three steps as follows: S1, a fibroin raw material and a cellulose raw material are dissolved in different NMMO / water mixed solvents respectively, and a fibroin solution and a cellulose solution are obtained; s2, transferring the fibroin solution and the cellulose solution into a reaction kettle, adding a modifier, vacuumizing to remove oxygen, heating to 50-85 DEG C, and stirring to react to obtain a composite spinning solution; the modifier is a small molecular compound containing an epoxy group or an oxygen-containing heterocyclic ring; s3, the composite spinning solution is extruded through a spinneret plate, fiber forming is conducted through a coagulating bath, drafting and winding are conducted, and the blending modified antifibrillated lyocell fiber is prepared. According to the invention, through in-situ modification of the lyocell fiber spinning solution, the prepared lyocell fiber has the intrinsic antifibrillation capability, the addition of an after-finishing process for treatment is not needed, meanwhile, the mechanical property of the fiber is improved, and the fiber is endowed with better skin-friendly property and wearing comfort.

The present invention discloses a method for improving the properties of composite meat protein gel based on modified pea protein, which belongs to the technical field of food protein processing. The present invention fully dissolves natural pea protein or pH-shifted modified pea protein to obtain a protein solution; then adds a disulfide bond cleaving agent to the protein solution, dissolves and mixes the solution to obtain modified pea protein. The present invention is based on the disulfide bond cleavage treatment of pea protein to dissociate acidic and basic subunits and expose more internal groups, thereby achieving the improvement of the solubility and functionality of pea protein itself. In addition, both natural pea protein and modified pea protein with disulfide bond cleavage can enhance the structural properties of low-salt meat protein gel under the action of cross-linking enzymes, develop low-salt meat protein gel with good gelation and network structure, and provide solutions and technical support for the quality deterioration of meat products under various circumstances.

The invention belongs to the technical field of biological medicines, and particularly discloses an insulin-like growth factor-1-linker polypeptide-collagen, and the linker polypeptide has an amino acid sequence as shown in SEQ ID No.1. The N end of the designed and synthesized linker polypeptide simulates an IGF-1 receptor structure and can be combined with IGF-1, the C end of the linker polypeptide is rich in lysine residues and can be covalently cross-linked to collagen through glutamine transaminase, the attachment time of the IGF-1 in endometrium is prolonged, and endometrium thickening and endometrium injury repair are effectively promoted. The protein solution for promoting endometrial injury repair provided by the invention can form a stable repair microenvironment on the surface of the endometrial, promote the number recovery of endometrial glands with intrauterine adhesion, reduce the fibrosis ratio and promote the thickening of thin endometrial, has the advantages of high histocompatibility, simplicity in preparation, obvious improvement effect and the like, and can be used for preparing a medicine for treating endometrial injury. The method has no potential toxic influence on cells and has a good clinical application prospect.

The invention provides a high-modulus strong-adhesion injectable hydrogel, and belongs to the field of biological materials, the hydrogel is the injectable hydrogel with the highest modulus at present, the modulus is up to 10.7 MPa, the modulus is adjustable, the adhesion strength also reaches 160kPa, and the hydrogel can well simulate tissues with different moduli such as skin and cartilage; meanwhile, the hydrogel can also resist swelling and lubricate. The invention provides a preparation method of the hydrogel, which comprises the following steps: mixing a silk protein solution of the wild silkworm silk and the modified polysaccharide in proportion, pre-crosslinking, and further crosslinking by utilizing temperature, so as to obtain the hydrogel with high modulus and strong adhesion. According to the hydrogel, firstly, fibroin and polysaccharide are mixed to form water-insoluble fluid gel, the water-insoluble fluid gel can be used for underwater injection in arthroscopic surgery, and then body temperature triggers assembly to form tough hydrogel in situ. In addition, the invention also proves that the hydrogel can be used for suture-free skin repair and can effectively avoid wound disintegration.

The present invention relates to an improved process for producing a product comprising a protein of interest (TP) capable of crystallizing under salt soluble conditions by crystallization of said TP. The present invention uses at least two different TP-containing protein feeds, which are mixed to produce a supersaturated protein solution in which TP crystallizes. One of the protein feeds must have a pH at least plus 0.1 to the pH (pHMCY, TP) providing the maximum crystallization yield of the TP, and one of the protein feeds must have a pH at most minus 0.1 to the pHMCY, TP.

The invention discloses a sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity and a preparation method and application thereof, belongs to the technical field of functional protein peptides, and particularly relates to a preparation method of the sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity, which comprises the following steps: heating a sea cucumber flower protein solution; the preparation method comprises the following steps: mixing sea cucumber with simulated gastric juice, reacting for 30-150 minutes, then adding simulated intestinal juice, reacting for 20-120 minutes, carrying out enzyme deactivation treatment, centrifuging, taking supernate, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; and mixing the sea cucumber flower self-assembled polypeptide with deionized water to prepare a sea cucumber flower self-assembled polypeptide solution, mixing the sea cucumber flower self-assembled polypeptide solution with a sodium selenite solution and a phosphate buffer solution, reacting for 20-60 minutes, washing with an ethanol solution, centrifugally collecting precipitate, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-selenium nano-carrier. The sea cucumber flower self-assembled polypeptide-selenium nano-carrier prepared by the invention has good antioxidant activity and potential of effectively delivering selenium to an organism.

The present invention discloses a livestock and poultry manure biochar-based fertilizer, comprising the following raw materials in parts by weight: 10-42% of dilute protein solution and collagenase-modified livestock and poultry manure biochar, 20-40% of urea, 7-25% of diammonium phosphate, 7-24% of potassium chloride or potassium sulfate, 0.5-1% of modified starch, and 6-23% of bentonite. The livestock and poultry manure is selected from one of chicken manure, cow manure, and pig manure. After the biochar is modified with collagenase, the surface of the biochar has more phenolic hydroxyl groups, carboxyl groups, aldehyde groups, and hydroxyl functional groups, which are effective for NH4 + , PO4 3‑ , K + , has a stronger adsorption effect, collagenase modification makes the protein distribution more uniform, and the generated groups form a stable chemical bond with the biochar surface: it improves the dispersibility of the modified biochar in aqueous solution and reduces agglomeration; it has better chemical stability in acidic and alkaline environments.

The invention aims to provide a method, equipment, medium and program product for determining acidity coefficient, the method comprises the following steps: constructing a simulation box corresponding to protein, the simulation box comprising the protein, a corresponding solvent and a buffer solution; performing molecular dynamics simulation on the simulation box by utilizing a trained machine learning force field to obtain corresponding track file information; and determining an acidity coefficient corresponding to each drippable site in the protein based on the track file information. A dynamic process of protein in a solution is accurately and efficiently simulated by utilizing a machine learning force field, and an accurate acidity coefficient is calculated based on a state of a protein solution system in simulation.

The invention relates to an industrial method, in particular to a method for preparing a universal functional protein nano-film on the surface of a base material, which comprises the following steps: (1) mixing a protein solution, an optional functional monomer and a photoinitiator solution to prepare a mixed solution; (2) soaking a base material in the mixed solution; and (3) irradiating with ultraviolet rays to obtain the protein nano-film on the surface of the base material. According to the universal method for continuously preparing the functional protein nano-film on the surface of the base material, rapid self-assembly aggregation and monomer polymerization of the protein can be carried out at the same time under ultraviolet induction, and the protein-functional polymer two-dimensional nano-film prepared on the surface of the base material is compact, low in roughness, high in transparency and good in application prospect. The biocompatibility is good.

The invention discloses a preparation method of soybean protein-phycocyanin double-protein oil gel type plant-based cheese, and belongs to the technical field of food processing. The method comprises the following steps: (1) preparing soybean protein-phycocyanin double proteins; (2) ultrasonic preparation of a double-protein solution; (3) compounding plant-based cheese raw materials; (4) stirring and homogenizing the compound liquid; and (5) heating, cooling and forming. The gel type plant-based cheese adopts a compounding mode of two plant proteins, integrates the nutritional advantages of the two proteins, can play a role of a cheese substitute, is green, safe, natural and healthy, reduces the occurrence risk of hypertension, hyperlipidemia, diabetes and other diseases caused by animal fat and the like, and promotes the development and application of plant-based food.

The invention provides a process for preparing a high-purity human blood coagulation factor IX from plasma. The process comprises the following steps: acquiring plasma; adding anion gel, adsorbing, and collecting an eluted protein solution; adding heparin sodium, filtering, ultra-filtering and dialyzing to obtain dialyzed protein liquid; adding an S / D solution; adding anion gel for secondary adsorption, and collecting eluted protein liquid II; performing filtration, ultrafiltration and dialysis, and collecting eluted protein liquid after dialysis; filtering is performed; carrying out affinity gel chromatography purification, collecting eluent, and carrying out ultrafiltration and dialysis; filtering, and adding a dialysate containing glycine for dialysis to obtain a human blood coagulation factor IX stock solution; adjusting the titer, and adding heparin sodium to obtain a diluted solution; pre-filtering the diluted liquid to obtain a semi-finished product of the human blood coagulation factor IX; sterilizing, filtering, sub-packaging, carrying out vacuum freeze drying, carrying out dry heat virus inactivation, inspecting and packaging. According to the invention, the production process is simplified, the cost is reduced, the activity of the finally obtained human blood coagulation factor IX is greater than 50IU / ml, and the specific activity is higher than 180IU / mg.

The invention discloses an amyloid-like protein mediated long-acting metal organic framework antifouling agent as well as a preparation method and application thereof. Belongs to the field of marine antifouling material preparation. The anti-fouling agent is based on MOF with an anti-fouling function, and amyloid-like protein is self-assembled on the surface of the MOF to form a compact protective shell layer. According to the preparation method, metal ions and organic ligands react and are connected through coordinate bonds to form an MOF structure, and MOF particles are obtained through centrifugation, collection, precipitation, washing and drying; under the action of a water-soluble organic reducing substance, the protein completely opens a disulfide bond to form an unfolded protein chain, and the protein chain is self-assembled to obtain an amyloid-like protein solution; and placing the MOF particles in an amyloid-like protein solution, and carrying out simple one-step incubation to obtain the amyloid-like protein mediated long-acting metal organic framework antifouling agent. The antifouling agent is prepared by adopting a novel green, high-stability, rapid and efficient mild modification method, and has the advantages of stability, durability, universality and high efficiency.

The application belongs to the technical field of food processing, and particularly relates to an oat protein-pectin-proanthocyanidin compound and a preparation method thereof. The oat protein is mixed with water, and an alkali liquor is added to adjust the pH to obtain a protein material solution for medium grinding. Then, pectin is added to the ground protein material solution, and proanthocyanidin powder is added after the pH is adjusted, and secondary medium grinding is performed to obtain an oat protein-pectin-proanthocyanidin solution. Then, hydrochloric acid is slowly added to adjust the pH to 6.8-7.0. Finally, the protein solution is centrifuged, and the supernatant is spray dried to obtain the oat protein-pectin-proanthocyanidin compound. The oat protein-pectin-proanthocyanidin compound prepared by the method is a brand-new protein system, and solves the problems of poor dispersibility, nutritional defects and poor emulsifying property of oat protein, and has a wide application prospect.

The invention belongs to the field of food, and particularly relates to a preparation method of hydrolyzed whey protein. The preparation method of the hydrolyzed whey protein provided by the invention comprises the following steps: performing high-pressure homogenization on a whey protein aqueous solution, hydrolyzing the whey protein aqueous solution subjected to high-pressure homogenization in the presence of an enzyme, and performing enzyme deactivation, wherein in the step of performing high-pressure homogenization on the whey protein aqueous solution, the pressure of high-pressure homogenization is 150 to 450 MPa, and the time of high-pressure homogenization is 20 to 35 minutes. According to the method, protein feed liquid containing whey protein is subjected to high-pressure homogenization treatment before hydrolysis, so that alpha-helix, beta-fold and other protein secondary structures can be fully changed, the hydrolysis reaction time is greatly shortened, the bitter taste of the whey protein hydrolysate can be improved, and the characteristics of a whey protein hydrolysate downstream product can be improved; the osmotic pressure of the formula milk is reduced, and the reconstituability of the formula milk powder is improved.

The invention discloses a fluorescence sensor for detecting thromboregulatory protein as well as a preparation method and a use method of the fluorescence sensor. The preparation method comprises the following steps: S1, adding a capture antibody of the thromboregulatory protein into an elisa plate; s2, adding the prepared bovine serum albumin; s3, adding prepared thrombus regulatory protein solutions with different concentrations; s4, adding a prepared detection antibody modified with alkaline phosphatase and used for detecting thromboregulatory protein; s5, adding a p-nitrophenyl disodium phosphate solution, and fully reacting; s6, a NO2-Eu-MOF nanosheet solution is prepared; s7, preparing a 1, 4-benzene diphosphonic acid solution; s8, adding a 1, 4-benzene diphosphonic acid solution into the NO2-Eu-MOF nanosheet solution, and detecting a fluorescence signal; and S9, adding the solution obtained in the step S8 into the solution obtained in the step S5, detecting the fluorescence intensity, and constructing a fluorescence intensity-concentration working curve. According to the invention, the concentration of thromboregulatory protein in the sample can be quantitatively analyzed.

The invention discloses composite hydrogel for co-embedding myricetin and epicatechin and a preparation method of the composite hydrogel, and belongs to the technical field of hydrogel microballoons.The preparation method comprises the steps that an azolla filliculoidas protein solution and a myricetin solution are self-assembled to form azolla filliculoidas protein-myricetin hydrogel; then adding an epicatechin solution into the prepared composite hydrogel, so as to form rumex hanus protein-myricetin-epicatechin composite hydrogel; and finally, adding the prepared composite hydrogel into the oxidized long-shaped rice starch solution to obtain the composite hydrogel for co-embedding the myricetin and the epicatechin. The formed composite hydrogel is relatively small in particle size and relatively high in stability, the core material embedding effect is good, and the requirement of common application of myricetin and epicatechin is met; hyaluronic acid in the dispersing agent enables particles to keep a proper distance through electrostatic repulsion, and a polylactic acid chain segment improves the steric hindrance effect, so that micelle agglomeration and precipitation are prevented, and the storage life is prolonged.

The invention provides a magnetic bead for IgG antibody biotin labeling and purification and an application method, and the magnetic bead is prepared by the following steps: 1) preparing rProtein A and rTurbo ID into a protein solution; (2) mixing the protein solution with the carboxyl magnetic beads to react, so that the protein is coupled to the carboxyl magnetic beads to obtain rProtein A and rTurbo ID protein coated magnetic beads; according to the magnetic bead disclosed by the invention, biotin labeling of an antibody can be realized by applying Turbo ID enzyme, only ATP and common biotin molecules need to be used, and the cost is very low; and the biotin labeling reaction is carried out on the magnetic beads, so that the purification and biotin labeling operation is simplified, and the operation is simple and convenient.

The present invention discloses a preparation method of antioxidant sturgeon protein peptides, comprising the following steps: S1, extraction of crude sturgeon skin protein solution; S2, extraction of crude sturgeon swim bladder protein; S3, extraction of crude sturgeon meat protein solution; S4, mixed enzymatic hydrolysis; S5, subjecting the obtained enzymatic hydrolysis product to enzyme inactivation treatment first to obtain an enzymatic hydrolysis solution, and then sequentially ultrafiltering and vacuum drying the enzymatic hydrolysis solution to obtain antioxidant sturgeon protein peptides. The present invention mixes the proteins extracted from different parts of sturgeon in a specific ratio, and then performs enzymatic hydrolysis. The neutral protease and papain added can act synergistically to more comprehensively hydrolyze proteins, generating small peptides with specific functions. At the same time, glutathione added during enzymatic hydrolysis can protect the sulfhydryl groups of the enzyme and maintain the enzyme activity, so that the finally obtained sturgeon protein peptides have good antioxidant properties.

The invention discloses a preparation method and application of phycocyanin-fucoxanthin composite nanoparticles, and is characterized by comprising the following steps: mixing a phycocyanin aqueous solution with the concentration of 2mg / mL and a fucoxanthin ethanol solution with the concentration of 10mg / mL according to the volume ratio of (200-20): 1, stirring for 20-40 minutes at the speed of 600-1,200 rpm, filtering, washing, and drying to obtain the phycocyanin-fucoxanthin composite nanoparticles. The phycocyanin-fucoxanthin composite nanoparticles have the advantages that the stability and the biological activity of fucoxanthin and phycocyanin can be improved at the same time, and the phycocyanin-fucoxanthin composite nanoparticles can be used for preparing inflammation inhibition drugs.

The application belongs to the technical field of dechlorination carrier and specifically provides a preparation method of a dechlorination active carrier, which comprises the following steps: 1) polyoxyethylene-polyoxypropylene block copolymer and modified protein solution are dissolved in deionized water, then cobalt nitrate, aluminum lactate and 2-aldehyde benzene boronic acid are added and stirred uniformly, reaction is carried out at 110-130 DEG C, centrifugation and drying are carried out to obtain a precursor; 2) tetrabutylphosphonium hydroxide and sodium aluminate are added into deionized water, TEOS and the precursor are added after mixing, stirring is carried out, crystallization is carried out at 165-175 DEG C, centrifugation and washing are carried out, and the obtained solid is dried and calcined to obtain the dechlorination active carrier. The carrier prepared by the application has the advantage of good dechlorination effect.

The invention discloses a method for extracting phycocyanin from spirulina, and belongs to the field of phycocyanin preparation. The method comprises the following steps: taking spirulina, adding water to obtain a spirulina suspension, performing freeze thawing, intermittent ultrasonic treatment and centrifugation, and collecting supernate to obtain a crude extract of phycocyanin; adding a (NH4) 2SO4 solution into the protein crude extract, standing, and centrifuging to obtain a salting-out precipitate; adding the salting-out precipitate into a mixture of ethylene glycol and inorganic salt, magnetically stirring and layering to obtain a protein solution; and carrying out ultrafiltration, centrifugation and freeze drying on the protein solution to obtain the spirulina phycocyanin. The purity of the phycocyanin can reach 1.0, the extraction rate can reach 69.87%, and the purity and the extraction rate of the phycocyanin extracted through the method are both improved. The extraction method provided by the invention has the advantages of simple process flow, low cost, high efficiency and strong practicability, and is suitable for large-scale industrial production of high-purity phycocyanin.

The invention relates to the technical field of protein glycosylation modification, in particular to a method for assisting protein glycosylation modification based on a static magnetic field. Comprising the following steps: preparing a protein solution with the concentration of 3-8%, and adjusting the pH value of the protein solution to 8-12; placing the protein solution in a 2.5-10mT static magnetic field to assist the protein in hydration reaction; after hydration is completed, carbohydrates are added and fully mixed for 20-40 min under the condition of magnetic stirring, and a protein-carbohydrate mixed solution is prepared; adjusting the pH of the protein-saccharide mixed solution to be the same as that in the first step; carrying out constant-temperature magnetic stirring on the protein-saccharide mixed solution under the conditions that the temperature is 75-85 DEG C and the rotating speed is 150-250 rpm for reaction, and keeping the pH value constant in the reaction process; and after the reaction is finished, cooling in an ice bath and freeze-drying. The method has the advantages that the grafting rate of the Maillard product is remarkably increased, the browning process can be inhibited, and the color deterioration of the glycosylation product is avoided.

The invention relates to the technical field of biological engineering, in particular to a coconut protein cavitation jet-enzymolysis combined extraction device which comprises a mixing hopper, a first tank body and a second tank body, a high-pressure pump and a first pump body are installed on the first tank body and the second tank body respectively, and a protein solution and an enzyme solution are contained in the first tank body and the second tank body respectively. The second pump body guides enzyme liquid into the mixing hopper through a pipeline, an impurity removal mechanism used for purifying mixed liquid generated by the protein solution and the enzyme liquid is arranged on the outer side of the mixing hopper, a mixing mechanism used for guiding nitrogen into the mixed liquid is further arranged on the mixing hopper, multiple layers of ultrafiltration membranes are arranged in the frame body, and a transmission assembly used for shaking the ultrafiltration membranes is arranged on the frame body. A protein solution driven by the high-pressure pump and an enzyme solution conveyed by the first pump body are combined to form a cavitation jet flow effect, high-speed jet flow can generate strong shearing force and turbulent flow, protein particles are broken, contact of enzyme and a substrate is accelerated, the enzymolysis reaction rate and uniformity are remarkably improved, and the extraction time is shortened.

The invention discloses genipin modified ginkgo protein and whey protein composite gel and a preparation method thereof, and belongs to the field of functional food. The method comprises the following steps: (1) uniformly mixing a prepared ginkgo protein solution and a prepared whey protein solution, and adjusting the pH value to obtain composite sol; (2) adding a genipin reagent into the composite sol, carrying out a cross-linking reaction, and after the reaction is completed, adjusting the pH value again to obtain a genipin-treated composite sol; and (3) carrying out thermal induction gel formation on the genipin-treated composite sol, taking out and cooling to obtain the genipin-modified ginkgo protein and whey protein composite gel. Animal protein and plant protein are combined through the cross-linking agent genipin, so that the prepared composite gel is more nutritional, pollution to the environment is reduced, application in food production is facilitated, meanwhile, the composite gel treated with the cross-linking agent genipin has more diversified functional characteristics, and the composite gel is suitable for being applied to food production. And the food can be provided for people with different requirements.

The invention discloses a purification method for removing endotoxin in prokaryotic expression recombinant protein, which belongs to the technical field of bioengineering, and comprises the following steps: adding 0.1% Triton X-114 in an ultrasonication stage, carrying out ice-bath ultrasonication, after the recombinant protein is combined with a Ni-NTA affinity column, washing with 50 column volumes of a combination buffer solution containing 0.1% Triton X-114, and carrying out elution by using an elution buffer solution containing 0.1% Triton X-114 in the presence of an elution buffer solution containing 0.1% Triton X-114 in the presence of an elution buffer solution containing 0.1% Triton X-114 in the presence of an elution buffer solution containing 0.1% Triton X-114; and washing with a 4-DEG C binding buffer solution with 20 column volumes, and subsequently eluting the target protein with imidazole with different gradients. Then, regulating the pH value of the purified recombinant protein solution to 2-4 or more than 10 through 100mM hydrochloric acid or sodium hydroxide, dissociating and decomposing endotoxin, carrying out oscillation treatment at room temperature for 2-4 hours, then removing the endotoxin in the recombinant protein through ultrafiltration, retaining an endotube protein solution, and detecting the content of protein and the endotoxin in the recombinant protein; the method is easy and convenient to operate and low in cost, the endotoxin content in the recombinant protein prepared through prokaryotic expression can be reduced by 400 times on the premise that immune recognition of the recombinant protein and serum is not affected, and the phenomenon that strong cross reaction occurs between immune serum and escherichia coli due to high endotoxin residues in the recombinant protein is reduced.

The invention relates to the technical field of peanut functional protein structure application, in particular to a peanut protein amyloid fiber and a preparation method thereof. The method specifically comprises the following steps: (1) mixing peanut meal with an organic solvent, degreasing to obtain degreased peanut powder, and preparing the degreased peanut powder into a degreased peanut powder solution; (2) centrifuging the degreased peanut powder solution for the first time, taking a supernatant, adjusting the pH value, standing, centrifuging for the second time, taking a precipitate, dialyzing, and freeze-drying to obtain peanut protein isolate; (3) hydrating the peanut protein isolate, centrifuging for the third time, taking supernatant to obtain a peanut protein isolate solution, adjusting the pH value, centrifuging for the fourth time, taking supernatant, and filtering to obtain a protein solution; and (4) carrying out fibrosis treatment on the protein solution to obtain the peanut protein amyloid fiber. The preparation method provided by the invention can improve the generation amount of the peanut protein amyloid fiber and increase the length of the fiber, and provides a theoretical basis for the application of the peanut protein amyloid fiber in the food industry.