1673 results about "Protein solution" patented technology

A soy protein product, which may be an isolate, produces transparent heat-stable solutions at low pH values and is useful for the fortification of soft drinks and sports drinks without precipitation of protein. The soy protein product is obtained by extracting a soy protein source material with an aqueous calcium salt solution to form an aqueous soy protein solution, separating the aqueous soy protein solution from residual soy protein source, adjusting the pH of the aqueous soy protein solution to a pH of about 1.5 to about 4.4 to produce an acidified clear soy protein solution, which may be dried, following optional concentration and diafiltration, to provide the soy protein product.

A method for specifically removing or isolating plasmin(ogen) or plasmin in presence of fibrinogen from a mixture containing plasmin(ogen) or plasmin by contacting the mixture with a rigid amino acid wherein the amino group of the amino acid and the carboxylic group of the amino acid are about 6–8 Angstroms, preferably about 7 Angstroms apart and the rigid amino acid is covalently bound to the support via the amino group of the amino acid.

Protein crystallization screening and optimization droplet actuators, systems and methods are provided. According to one embodiment, a screening droplet actuator is provided and includes: (a) a port for introduction of one or more crystallization reagents and/or one or more protein solutions; and (b) a substrate including: (i) an array of two or more mixing wells; and (ii) electric field mediated microfluidics for moving droplets comprising the crystallization reagents and protein solutions into the mixing wells. Optimization droplet actuators, systems including screening droplet actuators, methods of screening protein crystallization conditions, and methods of testing conditions for growing a crystal are also provided.

A delivery device applies a biocompatible and biodegradable barrier material to a tissue region, e.g., to seal a vascular puncture site. The material comprises two liquid components, which are pre-packaged in individual dispensers. Upon mixing, the liquid components cross-link to create a barrier matrix. A holder attaches to the delivery device. The holder mutually supports the first and second dispensers while the protein solution and polymer solution are conveyed from the dispensers into a fluid delivery channel. The protein and polymer solutions mix as a result of flow through the channel.

A method of preparing a macromolecule deterrent surface on a pharmaceutical package. In particular, the present invention relates to a method of preparing a protein deterrent surface on a pharmaceutical package by applying a coating or coatings directly to the pharmaceutical package that reduces the adsorption of proteins onto pharmaceutical packaging while not affecting the activity of the protein solution contained.

A process is provided for selectively removing protein aggregates from a protein solution in a normal flow (NFF) filtration process. Preferably, it relates to a process for selectively removing protein aggregates from a protein solution in a normal flow (NFF) filtration process and virus particles from a protein solution in a two-step filtration process. In a first step, a protein solution is filtered through one or more layers of adsorptive depth filters, charged or surface modified microporous membranes or a small bed of chromatography media in a normal flow filtration mode of operation, to produce a protein aggregate free stream. The aggregate free protein stream can then be filtered through one or more ultrafiltration membranes to retain virus particles at a retention level of at least 3 LRV and to allow passage therethrough of an aggregate free and virus free protein solution.

The present disclosure relates to an aqueous process for the preparation of a protein isolate and a hydrolyzed protein concentrate from an oilseed meal, optionally comprising:

• • mixing an oilseed meal with an aqueous solvent to form a slurry;

optionally treating the slurry with phytase y;

separating the slurry with a solid/liquid separation to form:

• a liquid phase, comprising the aqueous solvent, soluble protein and oil; and
• a solid phase comprising insoluble protein;

separating the liquid phase to form:

• an oil phase; and
• an aqueous protein phase;

subjecting the aqueous protein phase to membrane filtration to obtain a protein solution; and drying the protein solution to obtain the protein isolate

subjecting the insoluble protein to enzymatic hydrolysis, and

subjecting the hydrolyzed protein to membrane filtration to obtain an amino acid and peptide solution; and drying the amino acid and peptide solution to obtain the hydrolyzed protein concentrate.

A soy protein product, which may be an isolate, produces transparent heat-stable solutions at low pH values and is useful for the fortification of soft drinks and sports drinks without precipitation of protein. The soy protein product is obtained by extracting a soy protein source material with an aqueous calcium salt solution to form an aqueous soy protein solution, separating the aqueous soy protein solution from residual soy protein source, adjusting the pH of the aqueous soy protein solution to a pH of about 1.5 to about 4.4 to produce an acidified clear soy protein solution, which may be dried, following optional concentration and diafiltration, to provide the soy protein product.

The invention discloses a method for preparing fibroin protein hydrogel, comprising the following steps: obtaining fibroin protein solution with mass concentration of 1-30% by degumming, dissolving and dialyzing domestic silkworm silk; carrying out ultrasonic oscillation treatment on the fibroin protein solution at the temperature of 0-60 DEG C with ultrasonic power being 10-2000 watt and treatment time being 30-600 seconds; carrying out standing for 0-6 hours, thus obtaining the fibroin protein hydrogel. With the method of ultrasonic oscillation treatment adopted, no chemical cross linking agent is added and the fibroin protein solution can be turned into the hydrogel in a short time, thus greatly improving work efficiency; meanwhile, the obtained fibroin protein hydrogel features good biocompatibility and adaptability to biological and medical fields such as artificial skin, artificial cartilage, cell culture brackets, bioreactors, enzyme immobilization materials and the like.

The invention relates to a stabilizing agent for protein solutions, in particular to a long-acting protein solution stabilizing agent. The agent comprises 0.1-5g protein, 0.1-10g sugar, 0.1-10g organic matter, 0.01-0.1g preservative, and 10-200mmol buffer solution salt per 100ml water, and a pH (Potential of Hydrogen) value is 7.2-7.4. The agent has simple constituents, protects the stability of the protein, prevents the protein from degeneration, does not interfere with target reaction, and has general applicability to the most protein solutions. The agent has obvious effects, and can keep the effectiveness of the protein solutions for a long time, and the protein solutions are to be used for the fields of reagents for clinical diagnosis, life science research and the like.

The invention discloses a preparation method of a protein molecule engram film, comprising the following steps: 1. pretreatment of carrier sheet glass used for preparing a protein molecule engram film: putting a cover glass into a beaker, adding cleaning agent and deionized water, and processing by ultrasound; 2. preparation of the solution of template protein, functional monomer and crosslinking monomer: preparing template protein solution by phosphate buffer, adding water-solubility functional monomer, crosslinking monomer and initiator, and evenly mixing; 3. carrying out polymerization between sheet glasses to form a polymer film; 4. elution of template protein molecule: putting the molecule engram film into a beaker, adsorbing eluent in the beaker, removing the template protein molecule, and obtaining an engram hole matched with the template protein molecule. In the engram process of the invention, protein has small possibility of denaturation, and polymerization condition is simple and moderate. The prepared molecule engram film can be developed into a developing chip capable of detecting protein.

The invention relates to a fast preparation method of a biological affinity copper nanometer cluster, is applied to the detection of Hg<2+> content in a water sample, and belongs to the technical field of nanometer material preparation. A copper source solution and a protein solution are uniformly mixed; the pH of the solution is regulated to the alkalinity by alkali; oxidizing agents are dropped; heating incubation is carried out for a period of time; the copper nanometer cluster is prepared; the copper nanometer cluster is used for detecting the fluorescence intensity of an Hg<2+> standard solution or an Hg<2+> sample solution; the fluorescence intensity is substituted into a linear regression equation, and the Hg<2+> content in the sample is calculated. Due to strong oxidizing property and coordinating capability of oxidizing agents, the secondary structure in protein can be changed, copper-protein-oxidant complexes can be formed, the reducing capacity of the protein is enhanced, and the formation of the copper nanometer cluster is obviously accelerated. The copper nanometer cluster is used for the Hg<2+> detection in the water sample, and the method is obviously superior to the prior art in the aspects of analysis time, sensitivity, selectivity and cost.

Methods of stimulating collagen production, including stimulation of chondrocyte production, at the site of a defect. Methods include administering to the site of a defect at least two proteins from the group IL-1ra, sTNF-RI, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-β1, and sIL-1RII.

A method of screening protein crystal growth conditions on a nano or meso scale comprises providing a micro-electromechanical chip with a plurality of micro-chambers in the micro-electromechanical chip. A protein solution is placed into the micro-chambers by an automated jet type dispensing means with nano or meso scale precision. The protein crystal growth conditions of each of the micro-chambers is adjusted so that the protein crystal growth conditions of each of the micro-chambers differs. Crystallization of the protein solution in the micro-chambers is effected. Protein crystal growth in the micro-chambers is then observed.

The invention belongs to the technical field of emulsion preparation, and discloses a stable-protein high-internal-phase oil-in-water emulsion and a preparation method thereof. The method comprises the following steps: a protein is dispersed into water, after the protein is fully hydrated, an aqueous phase protein solution is obtained, an oil phase is added, emulsification is performed, and therefore the stable-protein high-internal-phase oil-in-water emulsion is obtained. The method disclosed by the invention does not need any coagulants or surfactants, and the obtained high-internal-phase oil-in-water emulsion has good viscoelasticity, high heat stability and storage stability, and an excellent protection or controlled release effect on the oil phase; and the emulsion has good gelling performance, strong plasticity and remoldability, facilitates further processing, and has good application prospects in the fields of chemical products, daily chemicals, pharmaceuticals, food and healthcare products.

The invention relates to a method for preparing fish scale collagen. The method comprises the following steps: fresh fish scales are taken as a material, undergo cleaning, drying and crushing, are soaked in NaOH solution for degreasing, and soaked in hydrochloric acid solution for deashing; the fresh fish scale is added with water of which the weight is 5 to 10 times of that of the fish scales, the pH value is adjusted to between 2 and 4 by acid solution, pepsin of which the weight accounting for the weight of the mixing solution is between 1 and 5 percent is added, the mixing solution undergoes enzymolysis at a low temperature for 4 to 10 hours, and enzyme is killed; enzymatic liquid is filtered twice to obtain extracting solutions, the extracting solutions are mixed, the mixing solution is decolored, sodium chloride of which the weight accounting for the weight of the mixing solution is between 5 and 15 percent is added into the mixing solution for overnight salting out, deposit is separated and collected, and acetic acid of which the weight accounting for the weight of the mixing solution is between 5 and 20 percent is added into the deposit for dissolution to obtain protein solution; the protein solution is dialyzed and desalted to obtain purified collagen liquid; and the collagen liquid is subjected to freeze-drying or spray-drying and micro-crushing to obtain solid particle. The method has the advantages of reasonable process, simple operation, safety, low cost, little loss of nutrient matters of the product, low ash content and high yield and purity of the collagen.

The invention discloses a method for continuously extracting and preparing concentrated protein, separated protein and proteolysis products from almond meal. The method comprises the following steps of: degreasing and debitterizing residual oil meal obtained after almond oil is mechanically and coldly pressed, and then extracting with a low-concentration NaCl phosphate buffer solution to obtain the concentrated protein; preparing almond separated protein from the concentrated protein solution by applying an ultrafiltration membrane technology; preparing a proteolysis-almond polypeptide mixed solution from the separated protein solution by applying an enzymolysis technology; and ultrafiltering the polypeptide mixed solution by applying a membrane technology to obtain multifunctional active almond peptide with different class level molecular weights in sequence. The method has the advantages of simple process, strong operability and easiness in control of extracting conditions and can finish the extraction and preparation of the almond concentrated protein, the separated protein and the proteolysis products in sequence; and the obtained products have high purity and wide application and can be directly used as food additives, nutrition reinforcers, health care products, medicaments, and the like needed by the protein foods and different processing foods.

The invention discloses a method for extracting selenium-containing protein from selenium-enriched yeast, which is characterized by comprising the following steps: (1) choosing the selenium-enriched yeast as raw materials, using a sodium hydroxide or potassium hydroxide solution with potential of hydrogen (pH) of 11 to 14 to dissolve the selenium-enriched yeast to obtain mixed liquid A; (2) placing the mixed liquid in a vibration machine of 250 r/minute at the temperature of 35 DEG C to be vibrated for 8 hours to 12 hours, and performing ultrasound on the mixed liquid for 30 minutes for twice in vibration processes; and (3) arranging the mixed liquid in a centrifugal machine of 4,000 to 20,000 r/min for 30 minutes after vibration is finished to extract supernatant, and adjusting pH of the supernatant to 7 to obtain a selenium-containing protein solution. The method improves wall broken rate and protein dissolving rate of yeast cells, extraction rate of selenium in the selenium-enriched protein solution is more than 70% when the selenium-enriched solution is not filtered and purified, and selenium-containing protein with organic selenium content more than 95 % can be obtained after the selenium-enriched solution is filtered and purified.

A meat or fish composition which retains moisture during cooking is provided. A concentrated aqueous acidic protein solution derived from animal muscle tissue is added to the meat or fish prior to cooking. The concentrated aqueous acidic protein solution comprises myofibrillar proteins and sarcoplasmic proteins substantially free of myofibrils and sarcomeres.

The invention relates to a preparation method of a high-gelation soybean protein, and belongs to the technical field of soybean protein processing. The method comprises the following steps: (1) mixing soybean protein isolate and water to obtain a protein solution, and performing ultrasound preprocessing on the protein solution; and (2) adjusting the pH and temperature of the protein solution subjected to ultrasound preprocessing in the step (1), adding transglutaminase for cross linking reaction, cooling the product after cross linkage to room temperature, and then concentrating and freeze drying to obtain the high-gelation soybean protein. The method has the beneficial effects that the needed equipment is simple and safe to operate; the enzyme needed in production is small in amount, short in enzymolysis time, low in cost and environmentally-friendly; the obtained soybean protein is high in gel strength, and meanwhile the gel stability is good; and the application range of the soybean protein in foodstuff is expanded.