152 results about "Protein solution" patented technology

The invention aims to provide a method, equipment, medium and program product for determining acidity coefficient, the method comprises the following steps: constructing a simulation box corresponding to protein, the simulation box comprising the protein, a corresponding solvent and a buffer solution; performing molecular dynamics simulation on the simulation box by utilizing a trained machine learning force field to obtain corresponding track file information; and determining an acidity coefficient corresponding to each drippable site in the protein based on the track file information. A dynamic process of protein in a solution is accurately and efficiently simulated by utilizing a machine learning force field, and an accurate acidity coefficient is calculated based on a state of a protein solution system in simulation.

The application belongs to the technical field of dechlorination carrier and specifically provides a preparation method of a dechlorination active carrier, which comprises the following steps: 1) polyoxyethylene-polyoxypropylene block copolymer and modified protein solution are dissolved in deionized water, then cobalt nitrate, aluminum lactate and 2-aldehyde benzene boronic acid are added and stirred uniformly, reaction is carried out at 110-130 DEG C, centrifugation and drying are carried out to obtain a precursor; 2) tetrabutylphosphonium hydroxide and sodium aluminate are added into deionized water, TEOS and the precursor are added after mixing, stirring is carried out, crystallization is carried out at 165-175 DEG C, centrifugation and washing are carried out, and the obtained solid is dried and calcined to obtain the dechlorination active carrier. The carrier prepared by the application has the advantage of good dechlorination effect.

The invention discloses spirulina protein-zein composite nanoparticles and a preparation method and application thereof.The preparation method comprises the steps that a zein solution is added into a spirulina protein solution, ethanol in the solution is removed through evaporation, insoluble substances are removed through centrifugation, and spirulina protein-zein composite nanoparticle dispersion liquid is obtained; and freeze-drying to obtain spirulina protein-zein composite nanoparticle powder. The preparation method of the spirulina protein-zein composite nano-particles is simple, low in cost, green and safe, and the obtained nano-particles can be kept stable in a neutral pH environment, have good biocompatibility, can be applied to efficient embedding of bioactive components, and have good application prospects. The light, heat and storage stability of the bioactive components are remarkably improved; and the oxidation resistance, tyrosinase inhibition and in-vitro release performance of the bioactive components are improved.

The application provides a high-stability salt-free meat protein beverage and a preparation method thereof, and relates to the field of food. The method comprises the following steps: extracting myofibrillar protein in chicken, washing the myofibrillar protein with water to remove salt in the myofibrillar protein, and obtaining salt-free myofibrillar protein; dissolving the salt-free myofibrillar protein in water to obtain a salt-free myofibrillar protein solution; and dissolving high-charge-density sodium carboxymethyl cellulose in the salt-free myofibrillar protein solution and uniformly mixing. The high-charge-density sodium carboxymethyl cellulose is combined with meat protein in a non-covalent form to form a complex, the charge property of the meat protein is changed, the self-assembly behavior of the myofibril is hindered, and the aggregation is inhibited, so that the water solubility of the meat protein is improved; the steric hindrance effect and the electrostatic repulsion of the system are greatly improved, so that the thermal stability of the meat protein is greatly improved, and the instability effect of the meat protein in the heat processing process is effectively avoided. The clear and transparent salt-free beverage product with good stability can be processed by using the method.

The invention provides a hermetia illucens protein-EGCG compound and a preparation method and application thereof, and belongs to the technical field of functional food, cosmetics or pharmaceutical preparations, and the preparation method comprises the following steps: mixing hermetia illucens larva powder with a sodium chloride solution, and stirring to extract protein; adjusting the pH value to 4.2-4.5 to precipitate protein, and cleaning and drying to obtain hermetia illucens protein powder; the method comprises the following steps: dissolving hermetia illucens protein powder in a PBS buffer solution to obtain a hermetia illucens protein solution, mixing the hermetia illucens protein solution with an EGCG solution, adjusting the pH value to 9, carrying out a stirring reaction, and carrying out dialysis and freeze-drying to obtain a hermetia illucens protein-EGCG covalent complex; and mixing the hermetia illucens protein solution with the EGCG solution, stirring for reaction, dialyzing, and freeze-drying to obtain the hermetia illucens protein-EGCG non-covalent complex. According to the invention, the EGCG is used for modifying the hermetia illucens protein, and the prepared emulsion stabilizer can improve the stability and oxidation resistance of the emulsion at the same time.

The invention discloses anthocyanin-protein composite powder with high stability as well as a preparation method and application of the anthocyanin-protein composite powder, and belongs to the technical field of biological food pigments. The preparation method comprises the following steps: mixing a black rice anthocyanin solution and a protein solution, stirring in a dark place, and freeze-drying to form the anthocyanin-protein composite powder. The anthocyanin-protein composite powder prepared by the method disclosed by the invention can be applied to the preparation of colored cold noodles, steamed buns and noodles, the stability of the black rice anthocyanin obtained by the method disclosed by the invention in tap water is improved, and the retention rate of the black rice anthocyanin in deionized water is also obviously increased; the starch is inhibited from aging to a certain extent, and the storage stability is improved. The anthocyanin in the prepared anthocyanin-protein composite powder is high in stability, the investment cost is low, and the anthocyanin-protein composite powder can be widely applied to the technical field of food processing and has wide application prospects in flour products.

The invention discloses a crispness increasing method for crispy flour products, and belongs to the field of crispy products. According to the invention, the casein solution and the wheat protein solution are firstly prepared and are used as dispersion phases to prepare the gel-like emulsion with the oil phase of 60-80%, so that the freezing resistance of the gel-like emulsion is improved, and then the gel-like emulsion is used as a pore-forming agent to be added into the paste, so that a new way for preparing the crispy flour product is developed. The gel-like emulsion is applied to the crispy flour product to replace a traditional leavening agent, so that the greenness of the manufacturing method of the crispy flour product can be realized, the oil content and the oil oxidation degree of the crispy flour product are reduced, the moisture and network integrity of dough is better maintained, the operation efficiency and convenience are improved, and the production cost is reduced. The color and luster of the crispy flour product can be effectively regulated and controlled, and technical innovation and quality improvement in the field of the crispy flour product are expected to be promoted.

Provided is a method for deactivating or removing viruses in a protein solution, the method including a step in which radiation treatment and virus removal membrane treatment or SD treatment are integrated and the conditions of these treatments are optimized to be mutually complementary. More specifically, the method includes: (a) a step in which a protein solution is treated by being irradiated with radiation so that the removal coefficient (LRV) for viruses with a virus particle diameter of less than 33 nm in the protein solution is at least 1.00; and (b1) a step in which viruses are removed from the treated protein solution by a virus removal membrane with an LRV of at least 4.00 for bacteriophage PP7, or (b2) a step in which the treated protein solution is brought into contact with a liquid mixture (SD liquid mixture) of an organic solvent and a surfactant and viruses are deactivated by organic solvent / surfactant treatment (SD treatment) so that the LRV for envelope viruses in the protein solution is at least 2.00. Also provided is a method for manufacturing a protein solution in / from which viruses have been deactivated / removed, the method including the abovementioned steps.

This invention discloses a protein solution filter. The housing has an inlet and a outlet. A filter plate is disposed inside the housing to filter the protein solution, and the outlet is located on one side of the filter plate. A receiving box is disposed at the bottom of the housing to collect the protein solution falling from the filter plate. A pushing device is disposed inside the housing and can move along the surface of the filter plate to push the filtered material on the filter plate to the outlet. The pushing device is equipped with a cleaning component. After pushing the filtered material on the filter plate, the pushing device reciprocates on the filter plate, and the cleaning component contacts the surface of the filter plate and cleans the mesh on the filter plate. This application has automatic pushing and mesh cleaning functions, and has the advantages of high-efficiency filtration, convenient cleaning, and resource recycling.

In one embodiment, the protein crystal growth monitoring device may comprise: a crystal growth module for growing protein crystals; and a monitoring module for monitoring the protein crystals growing in the crystal growth module. The crystal growth module may comprise: a reservoir for accommodating a precipitant; and a plurality of capillaries for accommodating a protein solution, each capillary having one end positioned inside the reservoir. The monitoring module may comprise: a light-emitting module located on one side of the crystal growth module and configured to emit light toward the plurality of capillaries; and a detection module located on the other side of the crystal growth module and configured to detect light emitted from the light-emitting module.

This application discloses an anti-inflammatory and anti-protein adhesion metal polyphenol protein bilayer coating, its preparation method, and its application. The preparation method includes the following steps: preparing an inner layer protein solution with deionized water and an inner layer polyphenol solution with ethanol, then mixing the inner layer protein solution and the inner layer polyphenol solution to obtain an inner layer coating mixture solution; preparing an outer layer protein solution, an outer layer polyphenol solution, and a metal ion solution with deionized water, then mixing the outer layer protein solution, outer layer polyphenol solution, and metal ion solution to obtain an outer layer coating mixture solution; completely immersing the pretreated substrate in the inner layer coating mixture solution and irradiating it with a UV lamp to obtain a modified substrate; placing the modified substrate in the outer layer coating mixture solution and continuing to irradiate it with a UV lamp to obtain a metal polyphenol protein bilayer coating. This application utilizes the outer layer for rapid antibacterial, anti-inflammatory, and anti-tear protein deposition, and the inner layer for deep ROS removal, oxygen production, and sustained anti-inflammatory effects.

This invention belongs to the field of food processing technology, specifically providing a method for preparing antioxidant zinc chelate peptides, comprising the following steps: inoculating a protein solution with a compound microbial agent for fermentation; the compound microbial agent includes *Lactobacillus plantarum* and *Saccharomyces cerevisiae*; after enzymatic hydrolysis and debittering of the fermentation broth, the antioxidant peptides are separated and dried to obtain the antioxidant peptides; the antioxidant peptides are dissolved in water to prepare a peptide solution, and a zinc source is added to the peptide solution to carry out a chelation reaction; the supernatant is collected, and the precipitate is collected by alcohol precipitation to obtain the antioxidant zinc chelate peptides. This method uses low molecular weight peptides prepared by dual-microbial synergistic multi-enzyme hydrolysis as chelating ligands, which chelate with zinc ions under mild conditions to maximize the preservation of biological activity, achieving a dual enhancement of chelation capacity and antioxidant activity, and achieving a unity of high chelation rate, high antioxidant activity, high stability and good flavor. The process is green, safe, and leaves no organic solvent residue, making it suitable for large-scale industrial production.

A composition and method for perfusing tissue. A perfusate is composed of an endothelial cell stabilizer. thrombocyte concentrate (TC). The perfusate may further comprise a protein solution that may be an albumin-based solution or fresh frozen plasma (FFP). The perfusate composition may further comprise red blood cells. The perfusate composition may be perfused through an organ that has been removed from an organ donor and is being prepared for transport and transplant into a recipient. The perfusate composition may be perfused through an organ using a machine perfusion device.

A method for preparing a composite plant protein, including: S1: mixing at least two kinds of plant proteins with water, adding compound alkali powder to a mixed solution and stirring, so as to obtain a protein stock solution with a pH of 10-10.5; the two kinds of plant proteins being rice protein and pea protein, or the rice protein and soy protein; and the compound alkali powder is sodium hydroxide and calcium hydroxide; S2: obtaining a protein solution with a pH of 6.8-7.0 by performing a high-pressure jet mill-ultrasonic treatment on the protein stock solution obtained from step S1, and adding citric acid to a treated protein stock solution; and S3: obtaining the composite plant protein by spray-drying the protein solution obtained from step S2.

The present disclosure provides a method of administering a low dose soluble protein to a subject in need thereof, wherein the method comprises (a) adding an intravenous solution stabilizer ("IVSS") to an isotonic aqueous solution in a container to form a pretreated container, (b) adding an amount from a protein stock solution to the pretreated container of step (a) to form a low dose protein solution, and (c) administering the low dose protein solution of step (b) to the subject. Optionally, the dose of the soluble protein to be administered to the subject is an ultra-low dose protein, wherein the low dose protein solution undergoes a further dilution step. In particular, the method of administering an ultra-low dose soluble protein comprises: (a) adding an IVSS to an isotonic aqueous solution in a first container to form a first pretreated container, (b) adding an amount from the protein stock solution to the first pretreated container to form a low dose protein solution; (c) adding an IVSS to an isotonic aqueous solution in a second container to form a second pretreated container; and (d) adding an amount of the low dose protein solution in step (b) to the second pretreated container of step (c) to form an ultra-low dose protein solution, and (e) administering the low dose protein solution of step (b) to an individual in need thereof.

The invention discloses a method for resisting freezing and thawing deterioration of glutinous quality of potatoes based on antifreeze protein, and belongs to the technical field of food processing and storage. The method comprises the following steps: firstly, cleaning fresh potatoes, and cutting into blocks; soaking the raw potato blocks or the cooked potato blocks in an antifreeze protein solution, and performing permeation treatment under a vacuum condition; and then taking out, draining and cryopreserving. According to the method disclosed by the invention, the combination of the anti-freezing protein and a vacuum infiltration technology is proposed for the first time, and the anti-freezing protein is effectively introduced into potato tissues through vacuum infiltration, so that the potato products can still maintain relatively high waxiness, elasticity and adhesiveness in multiple freezing and thawing cycles. The processing system provided by the invention can directionally solve the problem that the waxy quality is reduced after the potatoes are frozen and thawed, and a new technical path is provided for the field of potato freezing processing.

The invention discloses a walnut source DPP-IV inhibitory peptide as well as a preparation and screening method and application thereof. The walnut source DPP-IV inhibitory peptide comprises FPAG and / or LPSYQPTP, the preparation and screening method comprises the following steps: (1) adding walnut protein powder into deionized water, and fully dissolving to obtain a walnut protein solution; (2) adding hydrolyzed compound protease into the walnut protein solution for enzymolysis to obtain walnut protein hydrolysate freeze-dried powder; (3) re-suspending the walnut protein hydrolysate freeze-dried powder in deionized water, and performing ultrafiltration separation by using an ultrafiltration membrane with the molecular weight cutoff of 1kDa to obtain a component F1; step (4), performing peptide fragment sequence identification on the obtained F1 component through peptidomics; and step (5), screening from peptide fragment sequences obtained by identification through a bioinformatics technology. The walnut source DPP-IV inhibitory peptide screened by the invention can be used for preparing a medicine or food with a DPP-IV inhibition function, so that the problems that the existing walnut source DPP-IV inhibitor has many side effects and the like are solved.

To provide a method for adsorbing dropped protein to a base material having a titanium oxide layer on the surface at a high rate.SOLUTION: A method for adsorbing a protein to a substrate having a titanium oxide layer on a surface thereof, the method comprising: a protein solution dropping step of dropping a protein solution containing a protein onto the titanium oxide layer of the substrate; and a protein adsorption step of adsorbing the protein contained in the dropped protein solution onto the titanium oxide layer, wherein the protein solution contains 0.1 mg to 5 mg / mL of bovine serum albumin, and the substrate onto which the protein solution has been dropped is held for 85 minutes to 480 minutes in the protein adsorption step.SELECTED DRAWING: Figure 2

The invention relates to the technical field of active peptide chelate preparation, and discloses a high-chelation-rate colla corii asini peptide-metal ion chelate preparation method, which comprises: preparing a colla corii asini protein solution; adding a metal ion solution into the colla corii asini protein solution to carry out pre-reaction so as to construct a metal ion template; then adding a core function protease with a self-speed-limiting characteristic on a product, and carrying out synchronous enzymolysis-chelation reaction under the guidance of a template; and finally terminating the reaction to obtain a product. According to the method, oriented cutting of protease hydrolysis sites is realized by pre-constructing a metal ion template, and excessive hydrolysis of the product is avoided by utilizing the self-speed-limiting characteristic of the core function protease. Enzymolysis and chelation reaction are carried out synchronously, so that the process flow is obviously simplified, and the loss of intermediate products is avoided. The colla corii asini peptide-metal ion chelate prepared by the method has the technical effects of high chelation rate, concentrated molecular weight distribution and high utilization rate of raw material protein.

The invention discloses a recovery treatment method of biological agent sewage, and belongs to the technical field of water treatment. The recovery treatment method of the biological agent sewage comprises the following steps: pre-treating the sewage to remove large suspended particles and grease in the sewage; the pretreated sewage is fed into an electrophoresis device for electrophoresis treatment, protein in the sewage is separated through the electrophoresis device, and a protein-rich solution obtained after separation is collected; the protein-rich solution is fed into an ultrafiltration membrane device for ultrafiltration, and protein is intercepted and recycled; and feeding the sewage solution subjected to protein separation into a denitrification device for denitrification treatment. By adopting the method for recycling and treating the biological agent sewage, the problems that protein cannot be effectively recycled from the existing biological sewage and the ammonia nitrogen treatment effect is poor can be solved.

The invention discloses a preparation method of an antibacterial and mildew-proof coating for the inner wall of a sterile bottle, which comprises the following steps: thoroughly cleaning and disinfecting the sterile bottle through ultrasonic cleaning and high-temperature steam sterilization to ensure that no microbial residue exists in the bottle; activating the inner wall of the bottle by adopting a plasma cleaning machine; then, polylactic acid modified ovalbumin is dissolved in water, a glutamine transaminase cross-linking agent and a chitosan polymer are sequentially added to prepare a modified protein solution, and the mixture not only has excellent biocompatibility and antibacterial performance, but also can effectively prevent bacteria and mould from breeding; filtering the prepared solution, injecting the filtered solution into an activated sterile bottle, uniformly covering the inner wall of the bottle with the solution by using a spin coater, and carrying out curing treatment in a constant-temperature oven to finally form an antibacterial and mildew-proof coating; according to the method, the toxicity problem caused by chemical additives is avoided, meanwhile, the stability and durability of the coating are improved, the service life of the sterile bottle is greatly prolonged, and the protection performance of the sterile bottle is enhanced.

The invention discloses a soybean protein-phospholipid biomimetic membrane as well as a preparation method and application thereof. The biomimetic membrane is formed by three raw materials of soybean protein, soybean lecithin and a charged polymer through electrostatic interaction and a physical blending method. The soybean 11s protein solution, the lipidosome dispersion liquid and the charged polymer solution are mixed to obtain the mosaic structure biomimetic membrane which is formed by combining various chemical bond crosslinking and electrostatic interaction, and the process conditions are simple, efficient, convenient and environmentally friendly. The biomimetic membrane provided by the invention has a mosaic structure and the characteristics of the raw materials, so that the biomimetic membrane has high ion passing rate, strong ion selection capability, excellent monovalent cation and divalent cation separation capability and certain biodegradability. When the biomimetic membrane is applied to ion separation, the separation capacity of ions with different valence states can be effectively improved, the separation effect of sodium ions and magnesium ions is optimal, and the separation factor is as high as 21.28 and is 54.6 times that of a biomimetic membrane without charges.

The application discloses a kind of amyloid-like protein-mediated long-acting metal organic framework antifouling agent and preparation method and application thereof, and belongs to the field of marine antifouling agent material preparation.The antifouling agent is based on MOF with antifouling function, and amyloid-like protein self-assembles to form a dense protective shell layer on the surface of MOF.In the preparation method, metal ions and organic ligands are connected by coordination bond to form MOF structure, and MOF particles are obtained by centrifugation, collection, precipitation, washing and drying;under the action of water-soluble organic reducing substance, the disulfide bond of protein is completely opened to form unfolded protein chain, and amyloid-like protein solution is obtained by self-assembly;MOF particles are incubated in amyloid-like protein solution by simple one-step method to obtain amyloid-like protein-mediated long-acting metal organic framework antifouling agent.The application uses a new method of green, high stability, fast and efficient mild modification to prepare antifouling agent, which has stability, durability, universality and high efficiency.

The invention relates to the field of biological materials, in particular to a preparation method of needle-shaped silicon dioxide coated with protein on the surface, which comprises the following steps: S1, treating artificially synthesized needle-shaped silicon dioxide by using a sodium hydroxide aqueous solution; then modifying the amount of the needle-shaped silicon dioxide by using a 3-aminopropyl methyl diethoxy silane modifier; s2, preparing a protein solution; s3, coating the surface of the needle-shaped silicon dioxide with protein by using EDC and NHS; and S4, centrifuging, washing and drying. The method has the beneficial effects that the operation is simple and convenient, the reaction condition is mild (protein denaturation is avoided), the biocompatibility is excellent (non-toxic crosslinking agent residues are left), and the method is suitable for industrial production of biomedical carriers, cosmetic active ingredient loading and tissue engineering materials.

The invention discloses a preparation method of a protein-based micro-capsule coated emulsified feed, and relates to the technical field of feeds. The method comprises the following steps: S1, adding a functional core material into a protein solution, emulsifying and cross-linking to obtain a protein-based microcapsule suspension; s2, performing low-temperature pre-drying on the protein-based microcapsule suspension to obtain a pre-dried material, and performing pulse vacuum drying to obtain protein-based microcapsule particles; s3, uniformly mixing the protein-based microcapsule particles with corn flour and bean foil powder to obtain the protein-based microcapsule coated emulsified feed. According to the method, an optimized drying process of low-temperature pre-drying and pulse vacuum drying is adopted, premature deformation of protein is avoided in the low-temperature pre-drying stage, and due to the alternating action of pulse vacuum, moisture in a material is periodically and rapidly volatilized, shrinkage and breakage of a micro-capsule structure are reduced, and the embedding rate is effectively increased. The method can shorten the feed preparation period and is suitable for large-scale production.

The invention relates to a wet spinning preparation process of protein modified polyacrylonitrile fibers, and relates to the field of wet spinning preparation processes, the wet spinning preparation process comprises the following steps: S1, heating and softening a spinning stock solution, dropwise adding a protein solution, and fully dissolving to obtain a clear and transparent protein modified spinning solution; s2, the protein modified spinning solution is sequentially subjected to filtering, defoaming, spinning, coagulating bath and drafting, and then the protein modified polyacrylonitrile fiber is obtained; the spinning stock solution is an acrylonitrile-methyl acrylate-itaconic acid ternary copolymerized stock solution. The acrylonitrile-methyl acrylate-itaconic acid ternary copolymerized stock solution is adopted as a spinning matrix, the compatibility with natural protein is improved by introducing a polar group, and the protein is uniformly dispersed at a molecular level in cooperation with a mode of dropwise adding the protein solution after heating and softening, so that the protein agglomeration phenomenon is remarkably reduced, and the uniformity of the internal structure of the fiber is improved.

The application discloses a silk protein-based hydrogel optical fiber and a preparation method and application thereof, and belongs to the technical field of hydrogel optical fibers.The silk protein-based hydrogel optical fiber with a chemical-physical double crosslinking structure is constructed through a synergistic strategy of enzymatic crosslinking and ethanol solution post-processing; firstly, a degumming treatment is performed by using a sodium carbonate solution to prepare a silk protein solution; then, a core liquid and a coating liquid are respectively prepared by using the silk protein solution, a horseradish peroxidase solution and an H2O2 solution; the core liquid is injected into a gel, and then, an ethanol solution is used to strengthen the mechanical property of the core liquid; finally, the coating liquid is coated, and the silk protein-based hydrogel optical fiber with excellent mechanical property, low optical loss and good biocompatibility is obtained.

The invention discloses a sequential enzymatic-ionic synergistic soybean protein isolate modification method, which comprises the following steps: dispersing soybean protein isolate in water to prepare a protein solution, adding glutamine transaminase, carrying out heating reaction to inactivate enzyme to obtain an enzyme cross-linked protein solution, adding MgCl2 into the enzyme cross-linked protein solution, and carrying out heating reaction to induce protein aggregation to obtain a modified soybean protein isolate solution; and cooling to room temperature after the reaction is finished. The invention also discloses an application of the soybean protein product obtained by the sequential enzymatic-ionic synergistic soybean protein isolate modification method as a food additive. According to the method, the sensitization of the soybean protein can be remarkably reduced, meanwhile, the unique stomach resistance-intestinal efficient release dynamic characteristic is shown, and the small intestine peptide fragment release amount is promoted to be increased.

This invention provides a whey protein isolate-polyphenol complex, its preparation method, and its application, relating to the food industry. The preparation method includes the following steps: S1, weighing whey protein isolate, dissolving it in phosphate buffer solution, and fully hydrating it to obtain a protein solution; S2, adding H2O2 solution and ascorbic acid to the protein solution, mixing thoroughly, and adding polyphenols to perform a grafting reaction to obtain a product solution; S3, dialyzing the product solution to remove unreacted polyphenols, and freeze-drying the retained solution to obtain the whey protein isolate-polyphenol complex. Compared with natural whey protein isolate, the whey protein isolate-polyphenol complex exhibits significantly enhanced ABTS free radical scavenging ability, iron reducing power, emulsifying activity index, emulsifying stability index, foaming ability, and foam stability, indicating that the covalent grafting of polyphenols successfully endows the whey protein isolate with superior antioxidant properties, emulsifying properties, and foaming properties.