268 results about "Protein solution" patented technology

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

The invention discloses a sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity and a preparation method and application thereof, belongs to the technical field of functional protein peptides, and particularly relates to a preparation method of the sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity, which comprises the following steps: heating a sea cucumber flower protein solution; the preparation method comprises the following steps: mixing sea cucumber with simulated gastric juice, reacting for 30-150 minutes, then adding simulated intestinal juice, reacting for 20-120 minutes, carrying out enzyme deactivation treatment, centrifuging, taking supernate, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; and mixing the sea cucumber flower self-assembled polypeptide with deionized water to prepare a sea cucumber flower self-assembled polypeptide solution, mixing the sea cucumber flower self-assembled polypeptide solution with a sodium selenite solution and a phosphate buffer solution, reacting for 20-60 minutes, washing with an ethanol solution, centrifugally collecting precipitate, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-selenium nano-carrier. The sea cucumber flower self-assembled polypeptide-selenium nano-carrier prepared by the invention has good antioxidant activity and potential of effectively delivering selenium to an organism.

The invention aims to provide a method, equipment, medium and program product for determining acidity coefficient, the method comprises the following steps: constructing a simulation box corresponding to protein, the simulation box comprising the protein, a corresponding solvent and a buffer solution; performing molecular dynamics simulation on the simulation box by utilizing a trained machine learning force field to obtain corresponding track file information; and determining an acidity coefficient corresponding to each drippable site in the protein based on the track file information. A dynamic process of protein in a solution is accurately and efficiently simulated by utilizing a machine learning force field, and an accurate acidity coefficient is calculated based on a state of a protein solution system in simulation.

The invention discloses an amyloid-like protein mediated long-acting metal organic framework antifouling agent as well as a preparation method and application thereof. Belongs to the field of marine antifouling material preparation. The anti-fouling agent is based on MOF with an anti-fouling function, and amyloid-like protein is self-assembled on the surface of the MOF to form a compact protective shell layer. According to the preparation method, metal ions and organic ligands react and are connected through coordinate bonds to form an MOF structure, and MOF particles are obtained through centrifugation, collection, precipitation, washing and drying; under the action of a water-soluble organic reducing substance, the protein completely opens a disulfide bond to form an unfolded protein chain, and the protein chain is self-assembled to obtain an amyloid-like protein solution; and placing the MOF particles in an amyloid-like protein solution, and carrying out simple one-step incubation to obtain the amyloid-like protein mediated long-acting metal organic framework antifouling agent. The antifouling agent is prepared by adopting a novel green, high-stability, rapid and efficient mild modification method, and has the advantages of stability, durability, universality and high efficiency.

The application belongs to the technical field of food processing, and particularly relates to an oat protein-pectin-proanthocyanidin compound and a preparation method thereof. The oat protein is mixed with water, and an alkali liquor is added to adjust the pH to obtain a protein material solution for medium grinding. Then, pectin is added to the ground protein material solution, and proanthocyanidin powder is added after the pH is adjusted, and secondary medium grinding is performed to obtain an oat protein-pectin-proanthocyanidin solution. Then, hydrochloric acid is slowly added to adjust the pH to 6.8-7.0. Finally, the protein solution is centrifuged, and the supernatant is spray dried to obtain the oat protein-pectin-proanthocyanidin compound. The oat protein-pectin-proanthocyanidin compound prepared by the method is a brand-new protein system, and solves the problems of poor dispersibility, nutritional defects and poor emulsifying property of oat protein, and has a wide application prospect.

The invention belongs to the field of food, and particularly relates to a preparation method of hydrolyzed whey protein. The preparation method of the hydrolyzed whey protein provided by the invention comprises the following steps: performing high-pressure homogenization on a whey protein aqueous solution, hydrolyzing the whey protein aqueous solution subjected to high-pressure homogenization in the presence of an enzyme, and performing enzyme deactivation, wherein in the step of performing high-pressure homogenization on the whey protein aqueous solution, the pressure of high-pressure homogenization is 150 to 450 MPa, and the time of high-pressure homogenization is 20 to 35 minutes. According to the method, protein feed liquid containing whey protein is subjected to high-pressure homogenization treatment before hydrolysis, so that alpha-helix, beta-fold and other protein secondary structures can be fully changed, the hydrolysis reaction time is greatly shortened, the bitter taste of the whey protein hydrolysate can be improved, and the characteristics of a whey protein hydrolysate downstream product can be improved; the osmotic pressure of the formula milk is reduced, and the reconstituability of the formula milk powder is improved.

The invention provides a magnetic bead for IgG antibody biotin labeling and purification and an application method, and the magnetic bead is prepared by the following steps: 1) preparing rProtein A and rTurbo ID into a protein solution; (2) mixing the protein solution with the carboxyl magnetic beads to react, so that the protein is coupled to the carboxyl magnetic beads to obtain rProtein A and rTurbo ID protein coated magnetic beads; according to the magnetic bead disclosed by the invention, biotin labeling of an antibody can be realized by applying Turbo ID enzyme, only ATP and common biotin molecules need to be used, and the cost is very low; and the biotin labeling reaction is carried out on the magnetic beads, so that the purification and biotin labeling operation is simplified, and the operation is simple and convenient.

The application belongs to the technical field of dechlorination carrier and specifically provides a preparation method of a dechlorination active carrier, which comprises the following steps: 1) polyoxyethylene-polyoxypropylene block copolymer and modified protein solution are dissolved in deionized water, then cobalt nitrate, aluminum lactate and 2-aldehyde benzene boronic acid are added and stirred uniformly, reaction is carried out at 110-130 DEG C, centrifugation and drying are carried out to obtain a precursor; 2) tetrabutylphosphonium hydroxide and sodium aluminate are added into deionized water, TEOS and the precursor are added after mixing, stirring is carried out, crystallization is carried out at 165-175 DEG C, centrifugation and washing are carried out, and the obtained solid is dried and calcined to obtain the dechlorination active carrier. The carrier prepared by the application has the advantage of good dechlorination effect.

The invention discloses a preparation method of a white kidney bean protein-propylene glycol alginate composite carrier steady-state emulsion for enhancing alpha-amylase inhibitory activity, and belongs to the technical field of emulsion preparation. The method comprises the following steps: S1, mixing white kidney bean protein with water to prepare a protein solution; s2, mixing propylene glycol alginate with water to prepare a propylene glycol alginate solution, and then mixing the propylene glycol alginate solution with the protein solution prepared in the step S1 to prepare a water phase; s3, fat-soluble active substances are added into edible oil, and an oil phase is prepared; and S4, mixing the water phase and the oil phase, and carrying out high-speed dispersion and high-pressure homogenization to prepare the steady-state emulsion. The preparation method is simple and convenient to operate, and the prepared emulsion has good milk stability, storage stability, high active substance protection capacity and high alpha-amylase inhibitor activity.

The application discloses a preparation method of a high internal phase emulsion stabilized only by proteins, which mixes a completely hydrated protein solution as a water phase with an oil phase, and controls the hydrodynamic characteristics of a shearing emulsification process to obtain the high internal phase emulsion stabilized only by proteins. The preparation method of the application can obtain the high internal phase emulsion stabilized only by food-grade proteins through one-step shearing emulsification without other measures. The prepared high internal phase emulsion has good gel characteristics, stability and high safety, and can be used as a substitute for partially hydrogenated vegetable oil and saturated fatty acids. Meanwhile, the method is simple in operation, low in preparation cost and suitable for large-scale industrial promotion.

The invention discloses spirulina protein-zein composite nanoparticles and a preparation method and application thereof.The preparation method comprises the steps that a zein solution is added into a spirulina protein solution, ethanol in the solution is removed through evaporation, insoluble substances are removed through centrifugation, and spirulina protein-zein composite nanoparticle dispersion liquid is obtained; and freeze-drying to obtain spirulina protein-zein composite nanoparticle powder. The preparation method of the spirulina protein-zein composite nano-particles is simple, low in cost, green and safe, and the obtained nano-particles can be kept stable in a neutral pH environment, have good biocompatibility, can be applied to efficient embedding of bioactive components, and have good application prospects. The light, heat and storage stability of the bioactive components are remarkably improved; and the oxidation resistance, tyrosinase inhibition and in-vitro release performance of the bioactive components are improved.

This disclosure provides anti-VEGF antibodies formulated as high-concentration aqueous pharmaceutical compositions suitable for injection, preferably intravitreal injection. Aqueous pharmaceutical compositions are useful for delivering a high concentration of the antibody active ingredient to a patient without high levels of antibody aggregation and without a high level of sub-visible particulate matter. An aqueous composition of the disclosure comprises an antibody having a concentration of at least 50 mg / mL. An aqueous pharmaceutical composition of the disclosure includes a sugar, a buffering agent, and a surfactant.

The invention belongs to the field of preparation of Pickering emulsions, and particularly relates to a method for preparing a Pickering emulsifier from peanut protein. The preparation method of the emulsifier comprises the following steps: 1, preparing a peanut protein solution from peanut protein, and adjusting the pH value of the peanut protein solution to be neutral; step 2, heating the neutral peanut protein solution obtained in the step 1 at 90-100 DEG C; in order to further improve the stability of the Pickering emulsion in which the emulsifier participates in preparation, the preparation method of the emulsifier further comprises the following steps: step 3, heating, cooling to room temperature, and freeze-drying; carrying out superfine grinding on the freeze-dried peanut protein, redissolving and centrifuging to obtain soluble aggregate and insoluble aggregate; and 4, mixing the soluble aggregate and the insoluble aggregate according to a weight ratio of (0-1): 2. Wherein when the insoluble aggregate is completely used as the emulsifier to prepare the Pickering emulsion, the stability of the Pickering emulsion is optimal.

The invention discloses a modified cake meal protein-based degradable membrane material as well as a preparation method and application thereof. The membrane material is prepared from the following raw materials: a modifier and a cake meal protein solution, the modifier is prepared from the following raw materials: aryl diazacyclohexane and a three-arm star s-P (NIPAM-co-DMAM); the modifying agent and the cake protein form a three-dimensional cross-linked network structure through an electric field auxiliary curing technology, the prepared membrane material has excellent mechanical properties and controllable degradation characteristics, meanwhile, low-temperature efficient forming is achieved, production energy consumption is remarkably reduced, and an innovative solution is provided for high-value utilization of agricultural waste.

The application relates to the technical field of fabrics, in particular to a fabric based on protein fiber combined with natural fiber and a preparation method thereof, which is prepared by wet core-sheath composite spinning of a down protein sheath layer and a natural cellulose core layer; the core layer is natural cellulose fiber, and the total mass of the functional fabric is 70-90%; the balance is the sheath layer formed by cross-linking and solidification of a down protein solution; and the sheath layer is loaded with nano titanium dioxide; the introduced natural cellulose core layer is the main skeleton component of the fabric, selects natural fibers such as bamboo pulp cellulose and cotton linter pulp, and provides basic mechanical support for the fabric. The abandoned down resource is used to prepare protein fiber, which is combined with natural cellulose fiber to construct a composite fiber with a functional sheath layer and a mechanical core layer. The technical scheme solves the problems of insufficient sun protection, insufficient antibacterial performance and single mechanical performance of traditional natural fiber fabrics.

The application provides a high-stability salt-free meat protein beverage and a preparation method thereof, and relates to the field of food. The method comprises the following steps: extracting myofibrillar protein in chicken, washing the myofibrillar protein with water to remove salt in the myofibrillar protein, and obtaining salt-free myofibrillar protein; dissolving the salt-free myofibrillar protein in water to obtain a salt-free myofibrillar protein solution; and dissolving high-charge-density sodium carboxymethyl cellulose in the salt-free myofibrillar protein solution and uniformly mixing. The high-charge-density sodium carboxymethyl cellulose is combined with meat protein in a non-covalent form to form a complex, the charge property of the meat protein is changed, the self-assembly behavior of the myofibril is hindered, and the aggregation is inhibited, so that the water solubility of the meat protein is improved; the steric hindrance effect and the electrostatic repulsion of the system are greatly improved, so that the thermal stability of the meat protein is greatly improved, and the instability effect of the meat protein in the heat processing process is effectively avoided. The clear and transparent salt-free beverage product with good stability can be processed by using the method.

The application discloses an injectable microsphere gel of controllable performance silk protein and a preparation method thereof, and belongs to the field of biomaterials.The preparation method comprises the following steps: preparing a regenerated silk protein solution, including degumming, dissolving and purifying of mulberry silk, and obtaining a regenerated silk protein solution with a target molecular weight through molecular weight screening; preparing a silk protein microsphere aqueous solution by adding ethanol to the regenerated silk protein solution and performing freezing and thawing; obtaining a silk protein microsphere redispersible powder by drying two solutions prepared in the above steps; and preparing a silk protein microsphere gel by dispersing the silk protein microsphere redispersible powder in a solvent and adding a gel inducer.The whole preparation process does not involve toxic chemical reagents, and thus the excellent mechanical properties, ideal biocompatibility and biodegradability of the material are ensured; the silk protein microsphere gel has thixotropy, can be injected into subcutaneous tissue through a syringe, has a certain self-supporting property after injection, has controllable mechanical properties due to the molecular weight screening, and can be used in different application scenarios.

The present application mainly relates to a kind of preparation of sulfur-rich amino acid protein CMd-copper nanoclusters and its detection mercury ion method, comprising: a) by prokaryotic expression obtains CMd protein, and the protein concentration of 5 mg / mL-25 mg / mL CMd protein solution is obtained by purification.b) CMd is reacted with reducing agent TCEP after opening protein disulfide bond.c) the protein solution after reduction is reacted with anhydrous copper sulfate, and preparation high fluorescence CMd-copper nanocluster.d) CMd-copper nanocluster is applied to detect heavy metal mercury ion.The application method can be synthesized under mild aqueous conditions by "one-step method" structure controllable, stable CMd-copper nanocluster, widens the raw material of copper nanocluster synthesized with protein as template, avoids the use of organic solvent and strong reducing agent, and reduces the cost and environmental burden brought by noble metal raw material, and also has higher selectivity to mercury ion.

The invention provides a hermetia illucens protein-EGCG compound and a preparation method and application thereof, and belongs to the technical field of functional food, cosmetics or pharmaceutical preparations, and the preparation method comprises the following steps: mixing hermetia illucens larva powder with a sodium chloride solution, and stirring to extract protein; adjusting the pH value to 4.2-4.5 to precipitate protein, and cleaning and drying to obtain hermetia illucens protein powder; the method comprises the following steps: dissolving hermetia illucens protein powder in a PBS buffer solution to obtain a hermetia illucens protein solution, mixing the hermetia illucens protein solution with an EGCG solution, adjusting the pH value to 9, carrying out a stirring reaction, and carrying out dialysis and freeze-drying to obtain a hermetia illucens protein-EGCG covalent complex; and mixing the hermetia illucens protein solution with the EGCG solution, stirring for reaction, dialyzing, and freeze-drying to obtain the hermetia illucens protein-EGCG non-covalent complex. According to the invention, the EGCG is used for modifying the hermetia illucens protein, and the prepared emulsion stabilizer can improve the stability and oxidation resistance of the emulsion at the same time.

The invention discloses anthocyanin-protein composite powder with high stability as well as a preparation method and application of the anthocyanin-protein composite powder, and belongs to the technical field of biological food pigments. The preparation method comprises the following steps: mixing a black rice anthocyanin solution and a protein solution, stirring in a dark place, and freeze-drying to form the anthocyanin-protein composite powder. The anthocyanin-protein composite powder prepared by the method disclosed by the invention can be applied to the preparation of colored cold noodles, steamed buns and noodles, the stability of the black rice anthocyanin obtained by the method disclosed by the invention in tap water is improved, and the retention rate of the black rice anthocyanin in deionized water is also obviously increased; the starch is inhibited from aging to a certain extent, and the storage stability is improved. The anthocyanin in the prepared anthocyanin-protein composite powder is high in stability, the investment cost is low, and the anthocyanin-protein composite powder can be widely applied to the technical field of food processing and has wide application prospects in flour products.

The invention discloses a preparation method of low-sugar paper food, which is characterized in that sorghum is subjected to curing (baking) and enzymolysis to obtain low-sugar carbon water liquid; performing enzymolysis on soybean protein powder, sodium caseinate and papain, and performing sieving and colloid milling to obtain protein liquid; and adding the protein liquid, the low-sugar carbon water liquid, polyethylene glycol and medium-chain triglyceride, stirring, homogenizing and drying to obtain the paper food with the thickness of 0.1-3mm. The paper sheet food is thin in appearance, good in dissolubility, capable of being dissolved in oral saliva, fine and smooth, capable of being eaten without chewing and low in sugar content.

The invention discloses a crispness increasing method for crispy flour products, and belongs to the field of crispy products. According to the invention, the casein solution and the wheat protein solution are firstly prepared and are used as dispersion phases to prepare the gel-like emulsion with the oil phase of 60-80%, so that the freezing resistance of the gel-like emulsion is improved, and then the gel-like emulsion is used as a pore-forming agent to be added into the paste, so that a new way for preparing the crispy flour product is developed. The gel-like emulsion is applied to the crispy flour product to replace a traditional leavening agent, so that the greenness of the manufacturing method of the crispy flour product can be realized, the oil content and the oil oxidation degree of the crispy flour product are reduced, the moisture and network integrity of dough is better maintained, the operation efficiency and convenience are improved, and the production cost is reduced. The color and luster of the crispy flour product can be effectively regulated and controlled, and technical innovation and quality improvement in the field of the crispy flour product are expected to be promoted.

Provided is a method for deactivating or removing viruses in a protein solution, the method including a step in which radiation treatment and virus removal membrane treatment or SD treatment are integrated and the conditions of these treatments are optimized to be mutually complementary. More specifically, the method includes: (a) a step in which a protein solution is treated by being irradiated with radiation so that the removal coefficient (LRV) for viruses with a virus particle diameter of less than 33 nm in the protein solution is at least 1.00; and (b1) a step in which viruses are removed from the treated protein solution by a virus removal membrane with an LRV of at least 4.00 for bacteriophage PP7, or (b2) a step in which the treated protein solution is brought into contact with a liquid mixture (SD liquid mixture) of an organic solvent and a surfactant and viruses are deactivated by organic solvent / surfactant treatment (SD treatment) so that the LRV for envelope viruses in the protein solution is at least 2.00. Also provided is a method for manufacturing a protein solution in / from which viruses have been deactivated / removed, the method including the abovementioned steps.

This invention discloses a protein solution filter. The housing has an inlet and a outlet. A filter plate is disposed inside the housing to filter the protein solution, and the outlet is located on one side of the filter plate. A receiving box is disposed at the bottom of the housing to collect the protein solution falling from the filter plate. A pushing device is disposed inside the housing and can move along the surface of the filter plate to push the filtered material on the filter plate to the outlet. The pushing device is equipped with a cleaning component. After pushing the filtered material on the filter plate, the pushing device reciprocates on the filter plate, and the cleaning component contacts the surface of the filter plate and cleans the mesh on the filter plate. This application has automatic pushing and mesh cleaning functions, and has the advantages of high-efficiency filtration, convenient cleaning, and resource recycling.

In one embodiment, the protein crystal growth monitoring device may comprise: a crystal growth module for growing protein crystals; and a monitoring module for monitoring the protein crystals growing in the crystal growth module. The crystal growth module may comprise: a reservoir for accommodating a precipitant; and a plurality of capillaries for accommodating a protein solution, each capillary having one end positioned inside the reservoir. The monitoring module may comprise: a light-emitting module located on one side of the crystal growth module and configured to emit light toward the plurality of capillaries; and a detection module located on the other side of the crystal growth module and configured to detect light emitted from the light-emitting module.

This application discloses an anti-inflammatory and anti-protein adhesion metal polyphenol protein bilayer coating, its preparation method, and its application. The preparation method includes the following steps: preparing an inner layer protein solution with deionized water and an inner layer polyphenol solution with ethanol, then mixing the inner layer protein solution and the inner layer polyphenol solution to obtain an inner layer coating mixture solution; preparing an outer layer protein solution, an outer layer polyphenol solution, and a metal ion solution with deionized water, then mixing the outer layer protein solution, outer layer polyphenol solution, and metal ion solution to obtain an outer layer coating mixture solution; completely immersing the pretreated substrate in the inner layer coating mixture solution and irradiating it with a UV lamp to obtain a modified substrate; placing the modified substrate in the outer layer coating mixture solution and continuing to irradiate it with a UV lamp to obtain a metal polyphenol protein bilayer coating. This application utilizes the outer layer for rapid antibacterial, anti-inflammatory, and anti-tear protein deposition, and the inner layer for deep ROS removal, oxygen production, and sustained anti-inflammatory effects.

This invention belongs to the field of food processing technology, specifically providing a method for preparing antioxidant zinc chelate peptides, comprising the following steps: inoculating a protein solution with a compound microbial agent for fermentation; the compound microbial agent includes *Lactobacillus plantarum* and *Saccharomyces cerevisiae*; after enzymatic hydrolysis and debittering of the fermentation broth, the antioxidant peptides are separated and dried to obtain the antioxidant peptides; the antioxidant peptides are dissolved in water to prepare a peptide solution, and a zinc source is added to the peptide solution to carry out a chelation reaction; the supernatant is collected, and the precipitate is collected by alcohol precipitation to obtain the antioxidant zinc chelate peptides. This method uses low molecular weight peptides prepared by dual-microbial synergistic multi-enzyme hydrolysis as chelating ligands, which chelate with zinc ions under mild conditions to maximize the preservation of biological activity, achieving a dual enhancement of chelation capacity and antioxidant activity, and achieving a unity of high chelation rate, high antioxidant activity, high stability and good flavor. The process is green, safe, and leaves no organic solvent residue, making it suitable for large-scale industrial production.

Large scale produced water-soluble legume proteins and obtained by: Crushing legume seeds, if necessary, degreasing the crushed legume seeds; Mixing the crushed legume seeds with water to produce a legume slurry; Adjusting the pH value of the legume slurry to a pH value between 6.8 and 7.5, preferably between 7.0 and 7.4; Separating starch and fibers by centrifugation or filtration to produce an aqueous protein solution as supernatant; Adjusting the pH value of the separated protein solution to a pH value between 7.2 and 8, Ultrafiltrating the pH-adjusted protein solution; Diafiltrating the ultrafiltration retentate with water with a pH value of 7.5-8.2 to a conductivity of the diafiltrate of no more than 30% of the conductivity of the permeate without diafiltration, i.e., to a conductivity of 1-3 mS / cm; Obtaining the diafiltered ultrafiltration protein retentate; and drying, cooling or freezing the ultrafiltration retentate, and a method for its production.

A composition and method for perfusing tissue. A perfusate is composed of an endothelial cell stabilizer. thrombocyte concentrate (TC). The perfusate may further comprise a protein solution that may be an albumin-based solution or fresh frozen plasma (FFP). The perfusate composition may further comprise red blood cells. The perfusate composition may be perfused through an organ that has been removed from an organ donor and is being prepared for transport and transplant into a recipient. The perfusate composition may be perfused through an organ using a machine perfusion device.

A method for preparing a composite plant protein, including: S1: mixing at least two kinds of plant proteins with water, adding compound alkali powder to a mixed solution and stirring, so as to obtain a protein stock solution with a pH of 10-10.5; the two kinds of plant proteins being rice protein and pea protein, or the rice protein and soy protein; and the compound alkali powder is sodium hydroxide and calcium hydroxide; S2: obtaining a protein solution with a pH of 6.8-7.0 by performing a high-pressure jet mill-ultrasonic treatment on the protein stock solution obtained from step S1, and adding citric acid to a treated protein stock solution; and S3: obtaining the composite plant protein by spray-drying the protein solution obtained from step S2.