The invention relates to the technical field of
microorganism application, in particular to low-nucleic-acid
yeast protein as well as a preparation method and application thereof. The preparation method of the low-nucleic-acid
yeast protein comprises the following steps: (1) carrying out inactivation treatment on high-
protein yeast; (2) carrying out enzymolysis on the inactivated yeast by adopting
neutral protease,
glucanase, mannase and first
nuclease, and after
enzyme deactivation, separating and taking a heavy phase to obtain crude yeast protein; (3) performing high-pressure homogenization treatment on the crude yeast protein; and (4) carrying out enzymolysis on the crude yeast protein subjected to high-pressure homogenization treatment by adopting second
nuclease and
lipase, and after
enzyme deactivation, separating and taking a heavy phase to obtain the low-nucleic-acid yeast protein. The yeast protein with the
nucleic acid content of 1.0% or below is obtained by taking the high-protein yeast as a
raw material through inactivation, compound
enzyme enzymolysis, high-pressure homogenization treatment and secondary enzymolysis, is neutral and pure in taste, and can be widely applied to the fields of
nutrition, health and food such as
protein supplementation and protein substitution.