4 results about "PROTEASE M" patented technology

The invention discloses hydrolyzed protein for improving intestinal barrier and preventing diarrhea and a preparation method thereof, and belongs to the technical field of microorganisms and enzymes. According to the preparation method, a whey protein solution is subjected to enzymolysis through trypsin, then cross-linking is conducted through TG enzyme, secondary hydrolysis is conducted through natural field protease M, and finally the obtained hydrolyzed protein has the diarrhea preventing effect; furthermore, the peptide fragment screened from the hydrolyzed protein also has the function of improving the intestinal barrier. The protein peptide prepared by the method can improve the intestinal barrier function of a body and reduce the change of osmotic pressure so as to achieve the effect of preventing diarrhea.

The invention provides a compound capable of inhibiting the activity of SARS-CoV-2 main protease Mpro as well as a preparation method and application of the compound. The compound is of a structure as shown in a general formula (I) and has main protease Mpro inhibitory activity, preferably, the compound can interfere with replication of coronavirus SARS-CoV-2 in cells, and it is prompted that the compound has the effect of treating coronavirus infection.

This invention provides a drug for combating SARS-CoV-2 and antibacterial agents, and a method for preparing the same. The method involves reacting L-cysteine ​​and copper sulfate under alkaline conditions to prepare negatively charged nano-Cu2S. This nano-Cu2S reacts with the main protease M of SARS-CoV-2. pro and papain PL pro The nano-Cu2S binds to the main protease M of the SARS-CoV-2 virus to inhibit viral replication. pro and papain PL pro The dissociation constants between the components are all less than 0.001 nM. Simultaneously, nano-Cu2S exhibits antibacterial activity, inhibiting the proliferation of various bacteria. The synthesis process of the drugs for combating COVID-19 and antibacterial purposes according to this invention is simple and suitable for large-scale production. Nano-Cu2S has low toxicity and good biosafety, and can be widely used in the coatings and disinfection fields to inhibit the spread of viruses and bacteria.

The application discloses a hydrolyzed protein for improving intestinal barrier and preventing diarrhea and a preparation method thereof, and belongs to the technical field of microorganisms and enzymes. The whey protein solution is first subjected to trypsin enzymolysis, then subjected to TG enzyme crosslinking, and finally subjected to secondary hydrolysis by using Tenyo protease M, so that the prepared hydrolyzed protein has the effect of preventing diarrhea; further, the peptide screened from the hydrolyzed protein also has the function of improving intestinal barrier. The protein peptide prepared by using the method can improve the intestinal barrier function of the organism, reduce the change of osmotic pressure, and achieve the effect of preventing diarrhea.